MD11G1143300.v1.1

UDP-glucuronic acid decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001856
Physical Location & Seq
Forward (+)
13346998 .. 13351426
4429 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1143300.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGCAAACAATTCTACCAATGGGGAGCACCAGACCACAACAAAGCCTCCTCCTTTGCCATCCCCTCTGCGGTTTTCCAAGTTTTTTCAGTCTAATATGAGAATTTTGGTTACTGGAGGAGCTGGATTCATCGGTTCTCACCTGGTTGACAGGTTAATGGAAAATGAAAAGAATGAGGTTATTGTCGTTGATAACTACTTCACTGGCTCGAAGGACAATCTGAAAAAGTGGATTGGTCATCCCAGATTTGAGCTTATTCGTCATGATGTCACAGAGACATTGTTGGTCGAGGTTGATCGGATTTACCATCTTGCTTGCCCAGCTTCTCCAATTTTTTACAAATACAATCCTGTAAAGACGATAAAAACAAACGTGATTGGCACGCTGAACATGCTCGGACTTGCAAAGCGAGTTGGAGCAAGGATTCTGCTGACATCCACTTCAGAGGTATATGGAGATCCTCTTGTGCATCCACAACCTGAAAGCTACTGGGGTAATGTTAACCCAATTGGAGTAAGGAGCTGCTATGATGAGGGCAAGCGCGTTGCTGAGACCTTGATGTTCGACTATCATAGGCAGCATGGCATCGAAATACGTATTGCCAGAATATTCAATACATACGGCCCTCGCATGAATATTGATGATGGGCGCGTCGTCAGCAATTTTATAGCTCAAGCACTTCGTGATGAGCCTTTGACAGTTCAAAATCCCGGGACTCAAACTCGCAGTTTCTGCTACGTCTCTGACCTGGTTGATGGCCTCATTCGTCTGATGGAAGGAGAGCACACTGGACCCATAAACCTCGGAAACCCTGGTGAATTTACAATGCTTGAACTTGCAGAGACAGTGAAGGAGCTCATCAACCCTGAGGTGGAGATCAAGAGGGTGGAGAACACTCCAGACGATCCTAGACAGAGAAAACCCGACATCACAAAGGCAAAAGAATTGCTGGGATGGGAGCCGAAGATCAAGTTGCGCGAAGGCCTACCTCTCATGGAGGAGGATTTCCGATTGAGGCTCGGAGCAAACAAAAAGAAATGA

Protein Analysis

347

Amino Acids

39.26

Weight (kDa)

7.74

Isoelectric Point (pI)

29.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 33 - 291 1.2e-08 RmlD substrate binding domain
Epimerase PF01370 35 - 269 4.9e-47 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 35 - 144 2.3e-08 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 36 - 330 1.3e-50 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 36 - 187 3.9e-12 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 89 - 205 8.2e-07 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 543, 651, 978
AciI CCGC 1 cut(s) 70
AclWI GGATC 2 cut(s) 452, 899
AcsI RAATTY 2 cut(s) 102, 818
AcuI CTGAAG 1 cut(s) 426
AdeI CACNNNGTG 1 cut(s) 683
AfiI CCNNNNNNNGG 2 cut(s) 69, 871
AgsI TTSAA 3 cut(s) 613, 704, 833
AjnI CCWGG 3 cut(s) 141, 747, 811
AluBI AGCT 7 cut(s) 122, 253, 323, 486, 522, 671, 856
AluI AGCT 7 cut(s) 122, 253, 323, 486, 522, 671, 856
Alw21I GWGCWC 3 cut(s) 30, 786, 858
Alw26I GTCTC 4 cut(s) 269, 545, 745, 836
AlwI GGATC 2 cut(s) 452, 899
AlwNI CAGNNNCTG 1 cut(s) 732
Ama87I CYCGRG 1 cut(s) 710
AoxI GGCC 3 cut(s) 622, 757, 982
ApeKI GCWGC 2 cut(s) 522, 577
ApoI RAATTY 2 cut(s) 102, 818
AspLEI GCGC 3 cut(s) 543, 651, 978
AspS9I GGNCC 2 cut(s) 623, 791
AsuC2I CCSGG 2 cut(s) 711, 712
AsuHPI GGTGA 2 cut(s) 131, 827
AvaI CYCGRG 1 cut(s) 710
AvaII GGWCC 1 cut(s) 791
AxyI CCTNAGG 1 cut(s) 867
BanII GRGCYC 1 cut(s) 858
Bbv12I GWGCWC 3 cut(s) 30, 786, 858
BbvI GCAGC 2 cut(s) 509, 589
BccI CCATC 6 cut(s) 67, 315, 638, 749, 766, 948
BceAI ACGGC 1 cut(s) 637
BciT130I CCWGG 3 cut(s) 143, 749, 813
BcnI CCSGG 2 cut(s) 711, 712
BcoDI GTCTC 4 cut(s) 269, 545, 745, 836
BfaI CTAG 1 cut(s) 909
BisI GCNGC 2 cut(s) 523, 578
BlsI GCNGC 2 cut(s) 524, 579
Bme1390I CCNGG 5 cut(s) 143, 711, 712, 749, 813
Bme18I GGWCC 1 cut(s) 791
BmeT110I CYCGRG 1 cut(s) 710
BmgT120I GGNCC 2 cut(s) 623, 791
BmiI GGNNCC 2 cut(s) 793, 960
BmrFI CCNGG 5 cut(s) 143, 711, 712, 749, 813
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 2 cut(s) 478, 594
BmuI ACTGGG 1 cut(s) 499
BpmI CTGGAG 2 cut(s) 135, 882
BpuEI CTTGAG 1 cut(s) 657
BpuMI CCSGG 2 cut(s) 711, 712
BsaAI YACGTR 1 cut(s) 596
BsaI GGTCTC 1 cut(s) 545
BsaJI CCNNGG 3 cut(s) 710, 802, 811
BsaXI ACNNNNNCTCC 2 cut(s) 31, 61
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 871
Bse1I ACTGG 4 cut(s) 118, 208, 494, 793
Bse21I CCTNAGG 1 cut(s) 867
BseBI CCWGG 3 cut(s) 143, 749, 813
BseDI CCNNGG 3 cut(s) 710, 802, 811
BseGI GGATG 5 cut(s) 59, 238, 434, 469, 959
BseLI CCNNNNNNNGG 2 cut(s) 69, 871
BseMII CTCAG 2 cut(s) 540, 858
BseNI ACTGG 4 cut(s) 118, 208, 494, 793
BseRI GAGGAG 3 cut(s) 39, 132, 1013
BseXI GCAGC 2 cut(s) 509, 589
BseYI CCCAGC 2 cut(s) 319, 949
Bsh1236I CGCG 3 cut(s) 543, 651, 978
BshFI GGCC 3 cut(s) 624, 759, 984
BsiHKAI GWGCWC 3 cut(s) 30, 786, 858
BsiHKCI CYCGRG 1 cut(s) 710
BsiSI CCGG 1 cut(s) 711
BslFI GGGAC 1 cut(s) 727
BslI CCNNNNNNNGG 2 cut(s) 69, 871
BsmAI GTCTC 4 cut(s) 269, 545, 745, 836
BsmBI CGTCTC 1 cut(s) 745
BsmFI GGGAC 1 cut(s) 727
BsnI GGCC 3 cut(s) 624, 759, 984
Bso31I GGTCTC 1 cut(s) 545
BsoBI CYCGRG 1 cut(s) 710
Bsp1286I GDGCHC 3 cut(s) 30, 786, 858
Bsp143I GATC 5 cut(s) 295, 457, 876, 904, 966
BspACI CCGC 1 cut(s) 70
BspANI GGCC 3 cut(s) 624, 759, 984
BspCNI CTCAG 2 cut(s) 541, 859
BspFNI CGCG 3 cut(s) 543, 651, 978
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 793, 960
BspPI GGATC 2 cut(s) 452, 899
BspTNI GGTCTC 1 cut(s) 545
BsrI ACTGG 4 cut(s) 118, 208, 494, 793
BssECI CCNNGG 3 cut(s) 710, 802, 811
BssMI GATC 5 cut(s) 295, 457, 876, 904, 966
Bst2UI CCWGG 3 cut(s) 143, 749, 813
Bst4CI ACNGT 2 cut(s) 700, 847
BstAPI GCANNNNNTGC 1 cut(s) 732
BstBAI YACGTR 1 cut(s) 596
BstC8I GCNNGC 3 cut(s) 316, 383, 539
BstDEI CTNAG 2 cut(s) 549, 867
BstF5I GGATG 5 cut(s) 59, 238, 434, 469, 959
BstFNI CGCG 3 cut(s) 543, 651, 978
BstHHI GCGC 3 cut(s) 543, 651, 978
BstKTI GATC 5 cut(s) 298, 460, 879, 907, 969
BstMAI GTCTC 4 cut(s) 269, 545, 745, 836
BstMBI GATC 5 cut(s) 295, 457, 876, 904, 966
BstMWI GCNNNNNNNGC 4 cut(s) 320, 391, 657, 732
BstNI CCWGG 3 cut(s) 143, 749, 813
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 5 cut(s) 141, 709, 710, 747, 811
BstSNI TACGTA 1 cut(s) 596
BstUI CGCG 3 cut(s) 543, 651, 978
BstV1I GCAGC 2 cut(s) 509, 589
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
Bsu36I CCTNAGG 1 cut(s) 867
BsuRI GGCC 3 cut(s) 624, 759, 984
BtsCI GGATG 5 cut(s) 59, 238, 434, 469, 959
BtsIMutI CAGTG 3 cut(s) 201, 786, 852
Cac8I GCNNGC 3 cut(s) 316, 383, 539
CaiI CAGNNNCTG 1 cut(s) 732
CciI TCATGA 1 cut(s) 262
CfoI GCGC 3 cut(s) 543, 651, 978
Cfr13I GGNCC 2 cut(s) 623, 791
Cfr9I CCCGGG 1 cut(s) 710
CseI GACGC 1 cut(s) 640
CsiI ACCWGGT 2 cut(s) 141, 747
CviAII CATG 5 cut(s) 263, 391, 581, 631, 994
DdeI CTNAG 2 cut(s) 549, 867
DpnI GATC 5 cut(s) 297, 459, 878, 906, 968
DpnII GATC 5 cut(s) 295, 457, 876, 904, 966
DraIII CACNNNGTG 1 cut(s) 683
Ecl136II GAGCTC 1 cut(s) 856
Eco105I TACGTA 1 cut(s) 596
Eco147I AGGCCT 1 cut(s) 984
Eco24I GRGCYC 1 cut(s) 858
Eco31I GGTCTC 1 cut(s) 545
Eco47I GGWCC 1 cut(s) 791
Eco53kI GAGCTC 1 cut(s) 856
Eco57I CTGAAG 1 cut(s) 426
Eco81I CCTNAGG 1 cut(s) 867
Eco88I CYCGRG 1 cut(s) 710
EcoICRI GAGCTC 1 cut(s) 856
EcoRII CCWGG 3 cut(s) 141, 747, 811
EcoT38I GRGCYC 1 cut(s) 858
Esp3I CGTCTC 1 cut(s) 745
FaeI CATG 5 cut(s) 266, 394, 584, 634, 997
FaqI GGGAC 1 cut(s) 727
FatI CATG 5 cut(s) 262, 390, 580, 630, 993
Fnu4HI GCNGC 2 cut(s) 523, 578
FokI GGATG 5 cut(s) 46, 225, 421, 456, 966
FriOI GRGCYC 1 cut(s) 858
Fsp4HI GCNGC 2 cut(s) 523, 578
FspBI CTAG 1 cut(s) 909
GlaI GCGC 3 cut(s) 542, 650, 977
GluI GCNGC 2 cut(s) 523, 578
GsaI CCCAGC 2 cut(s) 323, 953
GsuI CTGGAG 2 cut(s) 135, 882
HaeIII GGCC 3 cut(s) 624, 759, 984
HapII CCGG 1 cut(s) 711
HgaI GACGC 1 cut(s) 640
HhaI GCGC 3 cut(s) 543, 651, 978
Hin1II CATG 5 cut(s) 266, 394, 584, 634, 997
Hin6I GCGC 3 cut(s) 541, 649, 976
HinP1I GCGC 3 cut(s) 541, 649, 976
HincII GTYRAC 2 cut(s) 148, 502
HindII GTYRAC 2 cut(s) 148, 502
HinfI GANTC 3 cut(s) 126, 424, 715
HpaI GTTAAC 1 cut(s) 502
HpaII CCGG 1 cut(s) 711
HphI GGTGA 2 cut(s) 131, 827
Hpy166II GTNNAC 2 cut(s) 148, 502
Hpy188I TCNGA 9 cut(s) 222, 300, 398, 445, 745, 771, 806, 1010, 1022
Hpy188III TCNNGA 4 cut(s) 263, 683, 880, 899
Hpy8I GTNNAC 2 cut(s) 148, 502
Hpy99I CGWCG 1 cut(s) 656
HpyAV CCTTC 4 cut(s) 205, 770, 844, 974
HpyCH4III ACNGT 2 cut(s) 700, 847
HpyCH4IV ACGT 3 cut(s) 372, 595, 738
HpyCH4V TGCA 3 cut(s) 404, 469, 839
HpyF10VI GCNNNNNNNGC 4 cut(s) 320, 391, 657, 732
HpyF3I CTNAG 2 cut(s) 549, 867
HpySE526I ACGT 3 cut(s) 372, 595, 738
Hsp92II CATG 5 cut(s) 266, 394, 584, 634, 997
HspAI GCGC 3 cut(s) 541, 649, 976
KspAI GTTAAC 1 cut(s) 502
Kzo9I GATC 5 cut(s) 295, 457, 876, 904, 966
LmnI GCTCC 7 cut(s) 25, 119, 416, 519, 853, 958, 1022
Lsp1109I GCAGC 2 cut(s) 509, 589
LweI GCATC 2 cut(s) 478, 594
MabI ACCWGGT 2 cut(s) 141, 747
MaeI CTAG 1 cut(s) 909
MaeII ACGT 3 cut(s) 372, 595, 738
MaeIII GTNAC 2 cut(s) 109, 268
MalI GATC 5 cut(s) 297, 459, 878, 906, 968
MboI GATC 5 cut(s) 295, 457, 876, 904, 966
MboII GAAGA 1 cut(s) 976
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 3 cut(s) 30, 786, 858
MluCI AATT 7 cut(s) 10, 102, 330, 507, 661, 818, 944
MlyI GAGTC 1 cut(s) 709
MmeI TCCRAC 1 cut(s) 394
MseI TTAA 2 cut(s) 155, 501
MspI CCGG 1 cut(s) 711
MspR9I CCNGG 5 cut(s) 143, 711, 712, 749, 813
MunI CAATTG 1 cut(s) 507
MvaI CCWGG 3 cut(s) 143, 749, 813
MvnI CGCG 3 cut(s) 543, 651, 978
MwoI GCNNNNNNNGC 4 cut(s) 320, 391, 657, 732
NciI CCSGG 2 cut(s) 711, 712
NdeII GATC 5 cut(s) 295, 457, 876, 904, 966
NlaIII CATG 5 cut(s) 266, 394, 584, 634, 997
NlaIV GGNNCC 2 cut(s) 793, 960
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 1 cut(s) 394
PagI TCATGA 1 cut(s) 262
PceI AGGCCT 1 cut(s) 984
PfeI GAWTC 2 cut(s) 126, 424
PkrI GCNGC 2 cut(s) 524, 579
PleI GAGTC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 709
Ppu21I YACGTR 1 cut(s) 596
Psp124BI GAGCTC 1 cut(s) 858
Psp6I CCWGG 3 cut(s) 141, 747, 811
PspFI CCCAGC 2 cut(s) 319, 949
PspGI CCWGG 3 cut(s) 141, 747, 811
PspN4I GGNNCC 2 cut(s) 793, 960
PspPI GGNCC 2 cut(s) 623, 791
PstNI CAGNNNCTG 1 cut(s) 732
PsuI RGATCY 1 cut(s) 457
SacI GAGCTC 1 cut(s) 858
SaqAI TTAA 2 cut(s) 155, 501
SatI GCNGC 2 cut(s) 523, 578
Sau3AI GATC 5 cut(s) 295, 457, 876, 904, 966
Sau96I GGNCC 2 cut(s) 623, 791
SchI GAGTC 1 cut(s) 709
ScrFI CCNGG 5 cut(s) 143, 711, 712, 749, 813
SduI GDGCHC 3 cut(s) 30, 786, 858
SexAI ACCWGGT 2 cut(s) 141, 747
SfaNI GCATC 2 cut(s) 478, 594
SinI GGWCC 1 cut(s) 791
SmaI CCCGGG 1 cut(s) 712
SmlI CTYRAG 1 cut(s) 672
SmoI CTYRAG 1 cut(s) 672
SnaBI TACGTA 1 cut(s) 596
Sse9I AATT 7 cut(s) 10, 102, 330, 507, 661, 818, 944
SseBI AGGCCT 1 cut(s) 984
SsiI CCGC 1 cut(s) 70
SspI AATATT 2 cut(s) 609, 637
SspMI CTAG 1 cut(s) 909
SstI GAGCTC 1 cut(s) 858
StuI AGGCCT 1 cut(s) 984
StyD4I CCNGG 5 cut(s) 141, 709, 710, 747, 811
TaaI ACNGT 2 cut(s) 700, 847
TaiI ACGT 3 cut(s) 375, 598, 741
TaqI TCGA 4 cut(s) 209, 288, 564, 588
TasI AATT 7 cut(s) 10, 102, 330, 507, 661, 818, 944
TfiI GAWTC 2 cut(s) 126, 424
Tru1I TTAA 2 cut(s) 155, 501
Tru9I TTAA 2 cut(s) 155, 501
TscAI CASTG 3 cut(s) 208, 793, 852
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 2 cut(s) 522, 577
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 118, 180, 647
TspMI CCCGGG 1 cut(s) 710
TspRI CASTG 3 cut(s) 208, 793, 852
VpaK11BI GGWCC 1 cut(s) 791
XapI RAATTY 2 cut(s) 102, 818
XceI RCATGY 1 cut(s) 394
XmaI CCCGGG 1 cut(s) 710
XspI CTAG 1 cut(s) 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.