Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008670

Physical Location & Seq

Genomic Coordinates Reverse (-)

8829392 .. 8835962

Gene Structure 6571 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1103300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1995 bp

ATGTCGGGTTTACTTAATTCTTCTCTAAACGGATCAGCCTCAAATATTCCAGACAATAGTGGGCGATTTGCGTCATCTTTCTCTGGTCAATCTGGTGCAGCTTCTCCTGTTTTTCATCACTCAGGAAGTATTCAGGGATTGCATAACATTCATGGGAGCTTCAGTGTTCCCAATATGCCAGGTACTTTAACATCAAGAAGTTCAACATTAAATAATGTTCCTTCCGGTGGAGTACAACAACCAACTGGAAGCCTCTCTGGCGGACGATTCACATCAAATAACCTTCCTGTTGCTTTGTCTCAGTTATCTCATGGAAGCTCCCATGGACATTCAGGAATCACTAATAGAGGAGGTATAAGTGTAGTAGGAAACCCTGGATTTAGTAGTAGCACAAATGGAATTGGCGGTTCTATTCCTGGGATTCTCCCTACATCTGCTGCAATTGGTAACCGGAATGCTGTTTCAGGGTTGGGCGTGTCCCCAATTTTGGGAAATGCTGGTCCTCGGATTACAAGTTCCATGGGAAACATGGTTGGTGGGGGCAACATTGGGAGGAGTATTAGCTCTGGTGGAGGTTTGTCAGTGCCTGGTCTTGCTTCTCGTCTAAATTTAAGTGCAAATAGTGGATCTGGAAGCTTAACTGTCCAAGGGCAAAATCGCTTGATGAGTGGTGTGCTACCACAAGGTTCTCCGCAAGTAATTTCGATGCTTGGAAATTCTTATCCTAATCCTGGAGGTCCACTTTCTCAAAGCCATGTCCAAGTGAATAACTTGAGCTCTATGGGAATAATGAATGATGTGAATTCTAATGACAGTTCTCCTTTTGACATCAATGATTTCCCTTCATTAACAAGTCGTCCTAGTTCTGCGGGAGGGCCCCAGGGGCAATTGGGTTCACTACGAAAACAAGGCCTTGGCGTTAGTCCTATTGTTCAACAGAACCAAGAGTTCAGCATCCAAAATGAGGACTTTCCAGCTTTACCAGGATTTAAAGGTGGTAATGCTGAATATGGGATTGATATGCACCAGAAAGAACAACTCCATGACAATGCTGTGTCAATGATGCAATCTCAGCACTTCTCAATGGGAAGGTCCTCAGGATTTAACTTGGGGGGTGCTTATTCTTCTCATAGGCCACAACAACAGCAACAACATGCTCCATCAGTCAGCAGTAGTGGTGTCTCGTTTTCACAAGTAAACAATCAGGATTTTCTCCATATGCATGGTTCAGACATATTCCCATCATCACATTCAACATATCATTCACAGACTAGTGGAGGACCTCCTGGCATTGGATTAAGACCTCTGAATTCTGCCAATTCAGTTTCTGGTATGGGTTCATATGACCAGCTTATTCAGCAGTATCAACAACAAAATCAATCCCAGTTTCGCCTGCAACAGATGTCAGCTGGAAATCAATCATTTAGGGATCAGGGAATGAAATCAATGCAGTCAGCGCAATCCGCTCCAGACCTATTTGGTTTGCTTGGATTGTTAAGTGTCATAAGGATGAGCGATCCTGATCTTACATCGCTTGCACTTGGAATTGATTTGACAACACTGGGATTAAACTTGAATTCAACAGAAAATCTTCACAAGACTTTTGGTTCCCCGTGGTCTGACGAGCCAGCTAAGGGTGATCCAGAGTTTGGTGTTCCTCAATGTTACTATACTAAACAACCGCCTGTACTACATCAAGGGTATTTTTCAAAGTTTTCTGTGGAAACGTTGTTCTATATCTTTTACAGCATGCCCAAAGACGAAGCCCAATTATATGCTGCCTATGAACTTAATAATAGAGGCTGGTTTTATCACAAGGAGCACCGTCTATGGTTCATAAGGGTACCGAACATGGAGCCACTTGTGAAGACCAACGCGTACGAGAAAGGATCTTATCACTGCTTTGATCCCAATACATTTGAAACAATCCGAAAGGATAATTTTGTGGTGCATTATGAGTTGTTGGAGAAGAGACCGACGTTACCACAGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000288 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006355 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0015074 GO:0016070 GO:0016071 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0030014 GO:0030015 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903506 GO:1990904 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

NOT2_3_5

Protein Analysis

665

Amino Acids

70.51

Weight (kDa)

7.13

Isoelectric Point (pI)

54.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NOT2_3_5_C	PF04153	532 - 655	9.8e-40	NOT2/NOT3/NOT5 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008670)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07705 AT1G07705
fragaria_vesca	FvH4_3g34620 FvH4_3g34620 FvH4_3g34620
malus_domestica	MD03G1103300.v1.1 MD11G1117100.v1.1
prunus_persica	Prupe.6G087300_v2.0.a1
pyrus_communis	pycom03g08390 pycom11g09830
rosa_chinensis	RchiOBHm_Chr5g0062331
rosa_laevigata	RLG00000035550
rosa_multiflora	Rmu_sc0006295.1_g000002 Rmu_ssc0000409.1_g000034
rosa_roxburghii	Rroxscaffold_1G00018350
rosa_rugosa	Rorug05G0348500 Rorug05G0348600 Rorug05G0348700
rosa_samantha	Rh5AG408400 Rh5BG422100 Rh5CG446100 Rh5DG437100
rosa_wichuraiana	Rw5G038480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1843
AccB1I	GGYRCC	1 cut(s)	1843
AccB7I	CCANNNNNTGG	1 cut(s)	1649
AccBSI	CCGCTC	1 cut(s)	1466
AccII	CGCG	1 cut(s)	1877
AciI	CCGC	6 cut(s)	261, 405, 692, 869, 1464, 1682
AclI	AACGTT	1 cut(s)	1727
AclWI	GGATC	7 cut(s)	40, 634, 1437, 1511, 1634, 1897, 1901
AcsI	RAATTY	5 cut(s)	607, 715, 802, 1309, 1576
AcuI	CTGAAG	1 cut(s)	145
AfaI	GTAC	5 cut(s)	184, 234, 1689, 1845, 1880
AfiI	CCNNNNNNNGG	8 cut(s)	227, 487, 488, 731, 964, 1292, 1634, 1649
AflIII	ACRYGT	1 cut(s)	1875
AgsI	TTSAA	7 cut(s)	204, 935, 1254, 1576, 1581, 1710, 1922
AhlI	ACTAGT	1 cut(s)	1271
AjnI	CCWGG	8 cut(s)	178, 373, 415, 586, 730, 879, 982, 1285
AloI	GAACNNNNNNTCC	4 cut(s)	499, 531, 1715, 1747
Alw21I	GWGCWC	2 cut(s)	779, 1824
Alw26I	GTCTC	3 cut(s)	303, 1186, 1966
AlwI	GGATC	7 cut(s)	40, 634, 1437, 1511, 1634, 1897, 1901
AlwNI	CAGNNNCTG	2 cut(s)	587, 1328
AoxI	GGCC	3 cut(s)	875, 910, 1133
ApaI	GGGCCC	1 cut(s)	879
ApeKI	GCWGC	3 cut(s)	98, 437, 1778
ApoI	RAATTY	5 cut(s)	607, 715, 802, 1309, 1576
Asp700I	GAANNNNTTC	1 cut(s)	1590
Asp718I	GGTACC	1 cut(s)	1843
AspLEI	GCGC	1 cut(s)	1459
AspS9I	GGNCC	6 cut(s)	500, 737, 875, 876, 1092, 1280
AsuHPI	GGTGA	1 cut(s)	1649
AvaII	GGWCC	4 cut(s)	500, 737, 1092, 1280
AxyI	CCTNAGG	1 cut(s)	1096
BaeGI	GKGCMC	1 cut(s)	879
BanI	GGYRCC	1 cut(s)	1843
BanII	GRGCYC	2 cut(s)	779, 879
BbsI	GAAGAC	1 cut(s)	1874
Bbv12I	GWGCWC	2 cut(s)	779, 1824
BbvI	GCAGC	3 cut(s)	110, 424, 1765
BccI	CCATC	2 cut(s)	1168, 1249
BciT130I	CCWGG	8 cut(s)	180, 375, 417, 588, 732, 881, 984, 1287
BcoDI	GTCTC	3 cut(s)	303, 1186, 1966
BcuI	ACTAGT	1 cut(s)	1271
BfaI	CTAG	2 cut(s)	861, 1272
BglI	GCCNNNNNGGC	2 cut(s)	258, 883
BisI	GCNGC	3 cut(s)	99, 438, 1779
BlsI	GCNGC	3 cut(s)	100, 439, 1780
Bme1390I	CCNGG	8 cut(s)	180, 375, 417, 588, 732, 881, 984, 1287
Bme18I	GGWCC	4 cut(s)	500, 737, 1092, 1280
BmgT120I	GGNCC	6 cut(s)	500, 737, 875, 876, 1092, 1280
BmiI	GGNNCC	5 cut(s)	877, 878, 1609, 1845, 1857
BmrFI	CCNGG	8 cut(s)	180, 375, 417, 588, 732, 881, 984, 1287
BmrI	ACTGGG	2 cut(s)	1378, 1571
BmsI	GCATC	3 cut(s)	696, 963, 1053
BmuI	ACTGGG	2 cut(s)	1378, 1571
BpiI	GAAGAC	1 cut(s)	1874
BpmI	CTGGAG	2 cut(s)	753, 1452
Bpu10I	CCTNAGC	1 cut(s)	1632
BpuEI	CTTGAG	1 cut(s)	793
BsaBI	GATNNNNATC	2 cut(s)	271, 1521
BsaI	GGTCTC	1 cut(s)	1966
BsaWI	WCCGGW	2 cut(s)	224, 450
BsaXI	ACNNNNNCTCC	2 cut(s)	148, 178
Bsc4I	CCNNNNNNNGG	8 cut(s)	227, 487, 488, 731, 964, 1292, 1634, 1649
Bse1I	ACTGG	3 cut(s)	250, 1384, 1566
Bse21I	CCTNAGG	1 cut(s)	1096
Bse8I	GATNNNNATC	2 cut(s)	271, 1521
BseBI	CCWGG	8 cut(s)	180, 375, 417, 588, 732, 881, 984, 1287
BseGI	GGATG	2 cut(s)	954, 1515
BseJI	GATNNNNATC	2 cut(s)	271, 1521
BseLI	CCNNNNNNNGG	8 cut(s)	227, 487, 488, 731, 964, 1292, 1634, 1649
BseMII	CTCAG	4 cut(s)	135, 314, 1085, 1110
BseNI	ACTGG	3 cut(s)	250, 1384, 1566
BseRI	GAGGAG	2 cut(s)	363, 568
BseSI	GKGCMC	1 cut(s)	879
BseXI	GCAGC	3 cut(s)	110, 424, 1765
BsgI	GTGCAG	1 cut(s)	117
Bsh1236I	CGCG	1 cut(s)	1877
BshFI	GGCC	3 cut(s)	877, 912, 1135
BshNI	GGYRCC	1 cut(s)	1843
BsiHKAI	GWGCWC	2 cut(s)	779, 1824
BsiSI	CCGG	2 cut(s)	225, 451
BsiWI	CGTACG	1 cut(s)	1878
BslFI	GGGAC	1 cut(s)	463
BslI	CCNNNNNNNGG	8 cut(s)	227, 487, 488, 731, 964, 1292, 1634, 1649
BsmAI	GTCTC	3 cut(s)	303, 1186, 1966
BsmFI	GGGAC	1 cut(s)	463
BsmI	GAATGC	1 cut(s)	460
BsnI	GGCC	3 cut(s)	877, 912, 1135
Bso31I	GGTCTC	1 cut(s)	1966
Bsp120I	GGGCCC	1 cut(s)	875
Bsp1286I	GDGCHC	3 cut(s)	779, 879, 1824
Bsp143I	GATC	8 cut(s)	32, 626, 1429, 1516, 1522, 1639, 1889, 1906
Bsp19I	CCATGG	2 cut(s)	322, 519
BspACI	CCGC	6 cut(s)	261, 405, 692, 869, 1464, 1682
BspANI	GGCC	3 cut(s)	877, 912, 1135
BspCNI	CTCAG	4 cut(s)	134, 313, 1084, 1109
BspFNI	CGCG	1 cut(s)	1877
BspLI	GGNNCC	5 cut(s)	877, 878, 1609, 1845, 1857
BspPI	GGATC	7 cut(s)	40, 634, 1437, 1511, 1634, 1897, 1901
BspT107I	GGYRCC	1 cut(s)	1843
BspTNI	GGTCTC	1 cut(s)	1966
BsrBI	CCGCTC	1 cut(s)	1466
BsrI	ACTGG	3 cut(s)	250, 1384, 1566
BssMI	GATC	8 cut(s)	32, 626, 1429, 1516, 1522, 1639, 1889, 1906
BssT1I	CCWWGG	4 cut(s)	322, 519, 646, 913
Bst2UI	CCWGG	8 cut(s)	180, 375, 417, 588, 732, 881, 984, 1287
Bst4CI	ACNGT	3 cut(s)	643, 815, 1826
Bst6I	CTCTTC	1 cut(s)	1964
BstC8I	GCNNGC	4 cut(s)	1394, 1536, 1629, 1751
BstDEI	CTNAG	5 cut(s)	121, 300, 1071, 1096, 1632
BstDSI	CCRYGG	3 cut(s)	322, 519, 1613
BstEII	GGTNACC	1 cut(s)	446
BstF5I	GGATG	2 cut(s)	954, 1515
BstFNI	CGCG	1 cut(s)	1877
BstHHI	GCGC	1 cut(s)	1459
BstKTI	GATC	8 cut(s)	35, 629, 1432, 1519, 1525, 1642, 1892, 1909
BstMAI	GTCTC	3 cut(s)	303, 1186, 1966
BstMBI	GATC	8 cut(s)	32, 626, 1429, 1516, 1522, 1639, 1889, 1906
BstMWI	GCNNNNNNNGC	6 cut(s)	258, 883, 1072, 1357, 1456, 1463
BstNI	CCWGG	8 cut(s)	180, 375, 417, 588, 732, 881, 984, 1287
BstNSI	RCATGY	2 cut(s)	1157, 1753
BstPI	GGTNACC	1 cut(s)	446
BstSCI	CCNGG	8 cut(s)	178, 373, 415, 586, 730, 879, 982, 1285
BstSLI	GKGCMC	1 cut(s)	879
BstUI	CGCG	1 cut(s)	1877
BstV1I	GCAGC	3 cut(s)	110, 424, 1765
BstV2I	GAAGAC	1 cut(s)	1874
BstX2I	RGATCY	2 cut(s)	626, 1889
BstXI	CCANNNNNNTGG	1 cut(s)	1223
BstYI	RGATCY	2 cut(s)	626, 1889
Bsu36I	CCTNAGG	1 cut(s)	1096
BsuRI	GGCC	3 cut(s)	877, 912, 1135
BtgI	CCRYGG	3 cut(s)	322, 519, 1613
BtgZI	GCGATG	1 cut(s)	1515
BtsCI	GGATG	2 cut(s)	954, 1515
BtsI	GCAGTG	1 cut(s)	1897
BtsIMutI	CAGTG	4 cut(s)	169, 588, 1559, 1897
Cac8I	GCNNGC	4 cut(s)	1394, 1536, 1629, 1751
CaiI	CAGNNNCTG	2 cut(s)	587, 1328
CfoI	GCGC	1 cut(s)	1459
Cfr13I	GGNCC	6 cut(s)	500, 737, 875, 876, 1092, 1280
CseI	GACGC	1 cut(s)	60
Csp6I	GTAC	5 cut(s)	183, 233, 1688, 1844, 1879
CviQI	GTAC	5 cut(s)	183, 233, 1688, 1844, 1879
DdeI	CTNAG	5 cut(s)	121, 300, 1071, 1096, 1632
DpnI	GATC	8 cut(s)	34, 628, 1431, 1518, 1524, 1641, 1891, 1908
DpnII	GATC	8 cut(s)	32, 626, 1429, 1516, 1522, 1639, 1889, 1906
DraI	TTTAAA	1 cut(s)	991
Eam1104I	CTCTTC	1 cut(s)	1964
EarI	CTCTTC	1 cut(s)	1964
EciI	GGCGGA	1 cut(s)	276
Ecl136II	GAGCTC	1 cut(s)	777
Eco130I	CCWWGG	4 cut(s)	322, 519, 646, 913
Eco147I	AGGCCT	1 cut(s)	912
Eco24I	GRGCYC	2 cut(s)	779, 879
Eco31I	GGTCTC	1 cut(s)	1966
Eco47I	GGWCC	4 cut(s)	500, 737, 1092, 1280
Eco53kI	GAGCTC	1 cut(s)	777
Eco57I	CTGAAG	1 cut(s)	145
Eco81I	CCTNAGG	1 cut(s)	1096
Eco91I	GGTNACC	1 cut(s)	446
EcoICRI	GAGCTC	1 cut(s)	777
EcoO109I	RGGNCCY	4 cut(s)	875, 876, 1092, 1280
EcoO65I	GGTNACC	1 cut(s)	446
EcoRI	GAATTC	3 cut(s)	802, 1309, 1576
EcoRII	CCWGG	8 cut(s)	178, 373, 415, 586, 730, 879, 982, 1285
EcoT14I	CCWWGG	4 cut(s)	322, 519, 646, 913
EcoT22I	ATGCAT	1 cut(s)	1224
EcoT38I	GRGCYC	2 cut(s)	779, 879
ErhI	CCWWGG	4 cut(s)	322, 519, 646, 913
FaqI	GGGAC	1 cut(s)	463
FauI	CCCGC	1 cut(s)	862
FauNDI	CATATG	2 cut(s)	1218, 1342
Fnu4HI	GCNGC	3 cut(s)	99, 438, 1779
FokI	GGATG	2 cut(s)	941, 1522
FriOI	GRGCYC	2 cut(s)	779, 879
Fsp4HI	GCNGC	3 cut(s)	99, 438, 1779
FspBI	CTAG	2 cut(s)	861, 1272
GlaI	GCGC	1 cut(s)	1458
GluI	GCNGC	3 cut(s)	99, 438, 1779
GsuI	CTGGAG	2 cut(s)	753, 1452
HaeIII	GGCC	3 cut(s)	877, 912, 1135
HapII	CCGG	2 cut(s)	225, 451
HgaI	GACGC	1 cut(s)	60
HhaI	GCGC	1 cut(s)	1459
Hin6I	GCGC	1 cut(s)	1457
HinP1I	GCGC	1 cut(s)	1457
HindIII	AAGCTT	1 cut(s)	634
HinfI	GANTC	3 cut(s)	267, 336, 421
HpaII	CCGG	2 cut(s)	225, 451
HphI	GGTGA	1 cut(s)	1649
Hpy166II	GTNNAC	4 cut(s)	11, 740, 896, 1198
Hpy188I	TCNGA	5 cut(s)	507, 1231, 1308, 1621, 1931
Hpy8I	GTNNAC	4 cut(s)	11, 740, 896, 1198
Hpy99I	CGWCG	1 cut(s)	1981
HpyAV	CCTTC	4 cut(s)	231, 293, 852, 1083
HpyCH4III	ACNGT	3 cut(s)	643, 815, 1826
HpyCH4IV	ACGT	2 cut(s)	1727, 1979
HpyF10VI	GCNNNNNNNGC	6 cut(s)	258, 883, 1072, 1357, 1456, 1463
HpyF3I	CTNAG	5 cut(s)	121, 300, 1071, 1096, 1632
HpySE526I	ACGT	2 cut(s)	1727, 1979
HspAI	GCGC	1 cut(s)	1457
KpnI	GGTACC	1 cut(s)	1847
Kzo9I	GATC	8 cut(s)	32, 626, 1429, 1516, 1522, 1639, 1889, 1906
LmnI	GCTCC	6 cut(s)	156, 323, 1162, 1471, 1819, 1855
Lsp1109I	GCAGC	3 cut(s)	110, 424, 1765
LweI	GCATC	3 cut(s)	696, 963, 1053
MaeI	CTAG	2 cut(s)	861, 1272
MaeII	ACGT	2 cut(s)	1727, 1979
MaeIII	GTNAC	3 cut(s)	446, 1664, 1980
MalI	GATC	8 cut(s)	34, 628, 1431, 1518, 1524, 1641, 1891, 1908
MbiI	CCGCTC	1 cut(s)	1466
MboI	GATC	8 cut(s)	32, 626, 1429, 1516, 1522, 1639, 1889, 1906
MboII	GAAGA	5 cut(s)	12, 1116, 1583, 1879, 1981
MfeI	CAATTG	2 cut(s)	441, 887
MflI	RGATCY	2 cut(s)	626, 1889
MhlI	GDGCHC	3 cut(s)	779, 879, 1824
MluI	ACGCGT	1 cut(s)	1875
MmeI	TCCRAC	1 cut(s)	1944
Mph1103I	ATGCAT	1 cut(s)	1224
MroXI	GAANNNNTTC	1 cut(s)	1590
MslI	CAYNNNNRTG	3 cut(s)	1047, 1221, 1508
MspA1I	CMGCKG	1 cut(s)	1409
MspI	CCGG	2 cut(s)	225, 451
MspR9I	CCNGG	8 cut(s)	180, 375, 417, 588, 732, 881, 984, 1287
MunI	CAATTG	2 cut(s)	441, 887
Mva1269I	GAATGC	1 cut(s)	460
MvaI	CCWGG	8 cut(s)	180, 375, 417, 588, 732, 881, 984, 1287
MvnI	CGCG	1 cut(s)	1877
MwoI	GCNNNNNNNGC	6 cut(s)	258, 883, 1072, 1357, 1456, 1463
NcoI	CCATGG	2 cut(s)	322, 519
NdeI	CATATG	2 cut(s)	1218, 1342
NdeII	GATC	8 cut(s)	32, 626, 1429, 1516, 1522, 1639, 1889, 1906
NlaIV	GGNNCC	5 cut(s)	877, 878, 1609, 1845, 1857
NsiI	ATGCAT	1 cut(s)	1224
NspI	RCATGY	2 cut(s)	1157, 1753
PaeI	GCATGC	1 cut(s)	1753
PasI	CCCWGGG	1 cut(s)	880
PceI	AGGCCT	1 cut(s)	912
PctI	GAATGC	1 cut(s)	460
PdmI	GAANNNNTTC	1 cut(s)	1590
PfeI	GAWTC	3 cut(s)	267, 336, 421
Pfl23II	CGTACG	1 cut(s)	1878
PflMI	CCANNNNNTGG	1 cut(s)	1649
PfoI	TCCNGGA	1 cut(s)	730
PkrI	GCNGC	3 cut(s)	100, 439, 1780
PpuMI	RGGWCCY	2 cut(s)	1092, 1280
Psp124BI	GAGCTC	1 cut(s)	779
Psp1406I	AACGTT	1 cut(s)	1727
Psp5II	RGGWCCY	2 cut(s)	1092, 1280
Psp6I	CCWGG	8 cut(s)	178, 373, 415, 586, 730, 879, 982, 1285
PspEI	GGTNACC	1 cut(s)	446
PspGI	CCWGG	8 cut(s)	178, 373, 415, 586, 730, 879, 982, 1285
PspLI	CGTACG	1 cut(s)	1878
PspN4I	GGNNCC	5 cut(s)	877, 878, 1609, 1845, 1857
PspOMI	GGGCCC	1 cut(s)	875
PspPI	GGNCC	6 cut(s)	500, 737, 875, 876, 1092, 1280
PspPPI	RGGWCCY	2 cut(s)	1092, 1280
PstNI	CAGNNNCTG	2 cut(s)	587, 1328
PsuI	RGATCY	2 cut(s)	626, 1889
PvuII	CAGCTG	1 cut(s)	1409
RsaI	GTAC	5 cut(s)	184, 234, 1689, 1845, 1880
RsaNI	GTAC	5 cut(s)	183, 233, 1688, 1844, 1879
RseI	CAYNNNNRTG	3 cut(s)	1047, 1221, 1508
SacI	GAGCTC	1 cut(s)	779
SatI	GCNGC	3 cut(s)	99, 438, 1779
Sau3AI	GATC	8 cut(s)	32, 626, 1429, 1516, 1522, 1639, 1889, 1906
Sau96I	GGNCC	6 cut(s)	500, 737, 875, 876, 1092, 1280
ScrFI	CCNGG	8 cut(s)	180, 375, 417, 588, 732, 881, 984, 1287
SduI	GDGCHC	3 cut(s)	779, 879, 1824
SfaNI	GCATC	3 cut(s)	696, 963, 1053
SinI	GGWCC	4 cut(s)	500, 737, 1092, 1280
SmiMI	CAYNNNNRTG	3 cut(s)	1047, 1221, 1508
SmlI	CTYRAG	1 cut(s)	772
SmoI	CTYRAG	1 cut(s)	772
SpeI	ACTAGT	1 cut(s)	1271
SphI	GCATGC	1 cut(s)	1753
SseBI	AGGCCT	1 cut(s)	912
SsiI	CCGC	6 cut(s)	261, 405, 692, 869, 1464, 1682
SspI	AATATT	1 cut(s)	46
SspMI	CTAG	2 cut(s)	861, 1272
SstI	GAGCTC	1 cut(s)	779
StuI	AGGCCT	1 cut(s)	912
StyD4I	CCNGG	8 cut(s)	178, 373, 415, 586, 730, 879, 982, 1285
StyI	CCWWGG	4 cut(s)	322, 519, 646, 913
TaaI	ACNGT	3 cut(s)	643, 815, 1826
TaiI	ACGT	2 cut(s)	1730, 1982
TaqI	TCGA	1 cut(s)	704
TaqII	GACCGA	1 cut(s)	1990
TatI	WGTACW	2 cut(s)	232, 1687
TfiI	GAWTC	3 cut(s)	267, 336, 421
TscAI	CASTG	4 cut(s)	169, 588, 1566, 1904
TseI	GCWGC	3 cut(s)	98, 437, 1778
TspDTI	ATGAA	8 cut(s)	104, 140, 806, 834, 1329, 1454, 1800, 1825
TspGWI	ACGGA	1 cut(s)	45
TspRI	CASTG	4 cut(s)	169, 588, 1566, 1904
Van91I	CCANNNNNTGG	1 cut(s)	1649
VpaK11BI	GGWCC	4 cut(s)	500, 737, 1092, 1280
XapI	RAATTY	5 cut(s)	607, 715, 802, 1309, 1576
XceI	RCATGY	2 cut(s)	1157, 1753
XcmI	CCANNNNNNNNNTGG	1 cut(s)	526
XmnI	GAANNNNTTC	1 cut(s)	1590
XspI	CTAG	2 cut(s)	861, 1272
Zsp2I	ATGCAT	1 cut(s)	1224

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.