Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0008670

Physical Location & Seq

Genomic Coordinates Reverse (-)

22570421 .. 22577027

Gene Structure 6607 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00018350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1995 bp

ATGTCGGGATTACTTAATTCTTCTCTAAACGGATCAGCCTCAAATCTTCCAGATAGTAGTGGACGTTTTGCCACATCTTTCTCTGGTCAATCAGGTGCTGCCTCTCCTGTATTTCATCACGCAGGATCTATTCAAGGATTGCATAACCTTCATGGGAGCTTCAATGTTCCTAACATGCCTGGTACATTAACTTCAAGAAACTCAACATTAACTAATGTTCCTTCTGGTGGAGTCCAACAACCAACTGGAAGCCTTTCTGGTGGACGATTTTCATCAAATAATCTTCCTGTTGCTCTGTCTCAGTTATCTCATGGAAGCTCTCATGGACATTCAGGAGTCACTAATAGAGGAGGTATAAGTGTAGTTGGAAACCCTGGATTTAGTAGTAGCACAAATGGAATTGGCGGTTCTATTCCTGGGATTCTCCCTACCTCTGCTGCAATTGGTAACCGTAATGCTGTTCCCGGATTGGGTGTAGGCCAAATTTTGGGAAATGCAGGTCCACGAATTACAAGTTCCATGGGAAACATGGTTGGTGGGGGCAACATTGGGAGGAGTATTAGTTCTGGTGGAGGCTTGTCGGTACCTGGTCTTACTTCCCGTCTAAATTTAAGTGGAAATAGTGGATCGGGTAGCTTAACTGTCCAAGGGCAGAATCGCTTGATGGGTGGTGTGCTTCCACAAGGGTCCCCGCAGGTTATTTCAATGTTGGGAAATTCTTATCCAAGTGCTGGAGGTCCACTTTCTCAAAGCCATGTCCAAGTGAATAATTTGAGCTCTATGGGAATGTTGAATGATGTAAATTCTAATGACAGTTCTCCTTTTGACATCAATGATTTCCCTCAATTAACAAGCCGGCCAAGTTCTGCGGGAGGCCCTCAAGGGCAATTGGGTTCATTGCGAAAACAAGGTCTTGGTGTTAGTCCAATTGTTCAACAAAACCAAGAGTTCAGCATCCAAAATGAAGATTTTCCGGCTTTGCCTGGATTTAAAGGTGGTAATTCTGATTATCCAATGGATATGCACCAGAAAGAACAACTTCATGACAATACCGTGTCAATGATGCAATCTCAGCACTTCTCAATGGGAAGATCTGCCGGATTTAACTTGGGAGGGACTTATTCATCTCATCGTCCACAGCAGCAACAACAACATGCTCCATCAGTCAGCAATAGTGGTGTCTCATTTTCTCAAGTAAACAATCAGGATCTTCTCCATTTGCATGGCTCAGATATGTTCCCATCATCGCATTCCACCTATCATTCACAGACAAGTGGACCTCCTGGCATTGGATTGAGACCTTTAAATTCGGCCAATACAGTTTCTGGCATGGGTTCTTATGACCAGCTTATCCAGCAGTATCAACAACACCAAAATCAGTCACAGTTTCGTCTGCAACAGATGTCACCGGTCAATCAATCGTTTAGGGATCAGGGAATTAAGTCGATGCAGACCTCGCAATCTGCTCCAGACCCATTTGGTTTGCTTGGTTTGTTAAGTGTCATAAGGATGAGTGATCCTGATTTAACATCTCTTGCACTTGGAATTGATTTGACAACGCTTGGCTTAAACTTGAATTCAACAGAAAATCTGCACAAGACTTTTGGTTCCCCATGGTCTGACGAGCCAGCTAAGGGTGATCCAGAGTTTAGTGTTCCCCAATGTTATTATGCTAAACAACCACCCGCACTACATCAAGGATATTTTTCAAAGTTCTCGGTGGAAACATTATTTTACATATTTTACAGCATGCCTAAAGATGAAGCTCAATTACATGCTGCATATGAACTTTATGACAGGGGCTGGTTCTATCACAAGGAGCACCATCTATGGATTGCAAGAGTCCCCAGTATGGAGCCACTTGTCAAGACAAACTCATATGAGAGAGGGTCTTACCACTGTTTTGATCCCAACACATTTGAAATAGTTCGAAAGGAAAATTTTGTTGTGCATTATGAAATGCTGGATAAGAGACCGTCCTTACCGCAGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000288 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006355 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0015074 GO:0016070 GO:0016071 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0030014 GO:0030015 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903506 GO:1990904 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

NOT2_3_5

Protein Analysis

664

Amino Acids

70.65

Weight (kDa)

6.83

Isoelectric Point (pI)

52.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NOT2_3_5_C	PF04153	532 - 655	1.3e-39	NOT2/NOT3/NOT5 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008670)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07705 AT1G07705
fragaria_vesca	FvH4_3g34620 FvH4_3g34620 FvH4_3g34620
malus_domestica	MD03G1103300.v1.1 MD11G1117100.v1.1
prunus_persica	Prupe.6G087300_v2.0.a1
pyrus_communis	pycom03g08390 pycom11g09830
rosa_chinensis	RchiOBHm_Chr5g0062331
rosa_laevigata	RLG00000035550
rosa_multiflora	Rmu_sc0006295.1_g000002 Rmu_ssc0000409.1_g000034
rosa_roxburghii	Rroxscaffold_1G00018350
rosa_rugosa	Rorug05G0348500 Rorug05G0348600 Rorug05G0348700
rosa_samantha	Rh5AG408400 Rh5BG422100 Rh5CG446100 Rh5DG437100
rosa_wichuraiana	Rw5G038480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	488, 685
Acc65I	GGTACC	1 cut(s)	583
AccB1I	GGYRCC	1 cut(s)	583
AccB7I	CCANNNNNTGG	2 cut(s)	487, 731
AciI	CCGC	5 cut(s)	405, 692, 869, 1686, 1985
AclWI	GGATC	8 cut(s)	40, 133, 634, 1215, 1437, 1511, 1634, 1901
AcoI	YGGCCR	2 cut(s)	857, 1311
AcsI	RAATTY	7 cut(s)	483, 607, 715, 802, 1306, 1576, 1939
AfaI	GTAC	2 cut(s)	184, 585
AfiI	CCNNNNNNNGG	7 cut(s)	227, 470, 487, 731, 1289, 1634, 1852
AgeI	ACCGGT	1 cut(s)	1408
AjnI	CCWGG	6 cut(s)	178, 373, 415, 586, 982, 1282
AloI	GAACNNNNNNTCC	2 cut(s)	547, 579
AluBI	AGCT	7 cut(s)	159, 318, 636, 777, 1348, 1631, 1766
AluI	AGCT	7 cut(s)	159, 318, 636, 777, 1348, 1631, 1766
Alw21I	GWGCWC	2 cut(s)	779, 1824
Alw26I	GTCTC	4 cut(s)	303, 1186, 1291, 1966
AlwI	GGATC	8 cut(s)	40, 133, 634, 1215, 1437, 1511, 1634, 1901
AlwNI	CAGNNNCTG	3 cut(s)	98, 1325, 1803
AoxI	GGCC	4 cut(s)	478, 857, 874, 1311
ApeKI	GCWGC	5 cut(s)	98, 437, 1141, 1778, 1987
ApoI	RAATTY	7 cut(s)	483, 607, 715, 802, 1306, 1576, 1939
ArsI	GACNNNNNNTTYG	2 cut(s)	895, 927
AsiGI	ACCGGT	1 cut(s)	1408
Asp700I	GAANNNNTTC	4 cut(s)	253, 969, 1038, 1926
Asp718I	GGTACC	1 cut(s)	583
AspS9I	GGNCC	5 cut(s)	500, 687, 737, 875, 1277
AsuC2I	CCSGG	1 cut(s)	465
AsuHPI	GGTGA	2 cut(s)	1398, 1649
AsuII	TTCGAA	1 cut(s)	1930
AvaII	GGWCC	4 cut(s)	500, 687, 737, 1277
BanI	GGYRCC	1 cut(s)	583
BanII	GRGCYC	1 cut(s)	779
BarI	GAAGNNNNNNTAC	4 cut(s)	175, 207, 1755, 1787
Bbv12I	GWGCWC	2 cut(s)	779, 1824
BbvI	GCAGC	4 cut(s)	85, 424, 1153, 1765
BccI	CCATC	4 cut(s)	658, 1168, 1249, 1833
BciT130I	CCWGG	6 cut(s)	180, 375, 417, 588, 984, 1284
BcnI	CCSGG	1 cut(s)	465
BcoDI	GTCTC	4 cut(s)	303, 1186, 1291, 1966
BfuAI	ACCTGC	2 cut(s)	488, 685
BglII	AGATCT	1 cut(s)	1091
BisI	GCNGC	5 cut(s)	99, 438, 1142, 1779, 1988
BlsI	GCNGC	5 cut(s)	100, 439, 1143, 1780, 1989
Bme1390I	CCNGG	7 cut(s)	180, 375, 417, 465, 588, 984, 1284
Bme18I	GGWCC	4 cut(s)	500, 687, 737, 1277
BmgT120I	GGNCC	5 cut(s)	500, 687, 737, 875, 1277
BmiI	GGNNCC	5 cut(s)	585, 688, 689, 1609, 1857
BmrFI	CCNGG	7 cut(s)	180, 375, 417, 465, 588, 984, 1284
BmrI	ACTGGG	1 cut(s)	1842
BmsI	GCATC	3 cut(s)	963, 1053, 1437
BmuI	ACTGGG	1 cut(s)	1842
BpmI	CTGGAG	2 cut(s)	753, 1452
Bpu10I	CCTNAGC	1 cut(s)	1632
Bpu14I	TTCGAA	1 cut(s)	1930
BpuEI	CTTGAG	2 cut(s)	864, 1176
BpuMI	CCSGG	1 cut(s)	465
BsaBI	GATNNNNATC	1 cut(s)	271
BsaI	GGTCTC	2 cut(s)	1291, 1966
BsaJI	CCNNGG	5 cut(s)	373, 416, 519, 646, 1613
BsaWI	WCCGGW	1 cut(s)	1408
BsaXI	ACNNNNNCTCC	2 cut(s)	547, 577
Bsc4I	CCNNNNNNNGG	7 cut(s)	227, 470, 487, 731, 1289, 1634, 1852
Bse118I	RCCGGY	2 cut(s)	855, 1408
Bse1I	ACTGG	2 cut(s)	250, 1848
Bse3DI	GCAATG	1 cut(s)	896
Bse8I	GATNNNNATC	1 cut(s)	271
BseBI	CCWGG	6 cut(s)	180, 375, 417, 588, 984, 1284
BseDI	CCNNGG	5 cut(s)	373, 416, 519, 646, 1613
BseGI	GGATG	2 cut(s)	954, 1515
BseJI	GATNNNNATC	1 cut(s)	271
BseLI	CCNNNNNNNGG	7 cut(s)	227, 470, 487, 731, 1289, 1634, 1852
BseMI	GCAATG	1 cut(s)	896
BseMII	CTCAG	3 cut(s)	314, 1085, 1242
BseNI	ACTGG	2 cut(s)	250, 1848
BseRI	GAGGAG	2 cut(s)	363, 568
BseXI	GCAGC	4 cut(s)	85, 424, 1153, 1765
BsgI	GTGCAG	1 cut(s)	1577
BshFI	GGCC	4 cut(s)	480, 859, 876, 1313
BshNI	GGYRCC	1 cut(s)	583
BshTI	ACCGGT	1 cut(s)	1408
BsiHKAI	GWGCWC	2 cut(s)	779, 1824
BsiSI	CCGG	5 cut(s)	465, 856, 974, 1098, 1409
BslFI	GGGAC	3 cut(s)	673, 1129, 1829
BslI	CCNNNNNNNGG	7 cut(s)	227, 470, 487, 731, 1289, 1634, 1852
BsmAI	GTCTC	4 cut(s)	303, 1186, 1291, 1966
BsmFI	GGGAC	3 cut(s)	673, 1129, 1829
BsmI	GAATGC	1 cut(s)	1249
BsnI	GGCC	4 cut(s)	480, 859, 876, 1313
Bso31I	GGTCTC	2 cut(s)	1291, 1966
Bsp119I	TTCGAA	1 cut(s)	1930
Bsp1286I	GDGCHC	2 cut(s)	779, 1824
Bsp143I	GATC	9 cut(s)	32, 125, 626, 1091, 1207, 1429, 1516, 1639, 1906
Bsp19I	CCATGG	2 cut(s)	519, 1613
BspACI	CCGC	5 cut(s)	405, 692, 869, 1686, 1985
BspANI	GGCC	4 cut(s)	480, 859, 876, 1313
BspCNI	CTCAG	3 cut(s)	313, 1084, 1241
BspHI	TCATGA	1 cut(s)	1042
BspLI	GGNNCC	5 cut(s)	585, 688, 689, 1609, 1857
BspMI	ACCTGC	2 cut(s)	488, 685
BspPI	GGATC	8 cut(s)	40, 133, 634, 1215, 1437, 1511, 1634, 1901
BspT104I	TTCGAA	1 cut(s)	1930
BspT107I	GGYRCC	1 cut(s)	583
BspTNI	GGTCTC	2 cut(s)	1291, 1966
BsrDI	GCAATG	1 cut(s)	896
BsrFI	RCCGGY	2 cut(s)	855, 1408
BsrI	ACTGG	2 cut(s)	250, 1848
BssAI	RCCGGY	2 cut(s)	855, 1408
BssECI	CCNNGG	5 cut(s)	373, 416, 519, 646, 1613
BssMI	GATC	9 cut(s)	32, 125, 626, 1091, 1207, 1429, 1516, 1639, 1906
BssT1I	CCWWGG	3 cut(s)	519, 646, 1613
Bst2UI	CCWGG	6 cut(s)	180, 375, 417, 588, 984, 1284
Bst4CI	ACNGT	8 cut(s)	452, 643, 815, 1054, 1321, 1386, 1901, 1977
BstBI	TTCGAA	1 cut(s)	1930
BstC8I	GCNNGC	3 cut(s)	857, 1629, 1751
BstDEI	CTNAG	4 cut(s)	300, 1071, 1228, 1632
BstDSI	CCRYGG	2 cut(s)	519, 1613
BstEII	GGTNACC	1 cut(s)	446
BstF5I	GGATG	2 cut(s)	954, 1515
BstKTI	GATC	9 cut(s)	35, 128, 629, 1094, 1210, 1432, 1519, 1642, 1909
BstMAI	GTCTC	4 cut(s)	303, 1186, 1291, 1966
BstMBI	GATC	9 cut(s)	32, 125, 626, 1091, 1207, 1429, 1516, 1639, 1906
BstMWI	GCNNNNNNNGC	3 cut(s)	1072, 1354, 1456
BstNI	CCWGG	6 cut(s)	180, 375, 417, 588, 984, 1284
BstNSI	RCATGY	4 cut(s)	178, 1157, 1753, 1778
BstPI	GGTNACC	1 cut(s)	446
BstSCI	CCNGG	7 cut(s)	178, 373, 415, 463, 586, 982, 1282
BstV1I	GCAGC	4 cut(s)	85, 424, 1153, 1765
BstX2I	RGATCY	3 cut(s)	125, 1091, 1207
BstXI	CCANNNNNNTGG	1 cut(s)	1223
BstYI	RGATCY	3 cut(s)	125, 1091, 1207
BsuRI	GGCC	4 cut(s)	480, 859, 876, 1313
BtgI	CCRYGG	2 cut(s)	519, 1613
BtgZI	GCGATG	1 cut(s)	1230
BtsCI	GGATG	2 cut(s)	954, 1515
BtsIMutI	CAGTG	1 cut(s)	1897
BveI	ACCTGC	2 cut(s)	488, 685
Cac8I	GCNNGC	3 cut(s)	857, 1629, 1751
CaiI	CAGNNNCTG	3 cut(s)	98, 1325, 1803
CciI	TCATGA	1 cut(s)	1042
Cfr10I	RCCGGY	2 cut(s)	855, 1408
Cfr13I	GGNCC	5 cut(s)	500, 687, 737, 875, 1277
CsiI	ACCWGGT	1 cut(s)	586
Csp6I	GTAC	2 cut(s)	183, 584
CspAI	ACCGGT	1 cut(s)	1408
CviQI	GTAC	2 cut(s)	183, 584
DdeI	CTNAG	4 cut(s)	300, 1071, 1228, 1632
DpnI	GATC	9 cut(s)	34, 127, 628, 1093, 1209, 1431, 1518, 1641, 1908
DpnII	GATC	9 cut(s)	32, 125, 626, 1091, 1207, 1429, 1516, 1639, 1906
DraI	TTTAAA	2 cut(s)	991, 1305
EaeI	YGGCCR	2 cut(s)	857, 1311
Ecl136II	GAGCTC	1 cut(s)	777
Eco130I	CCWWGG	3 cut(s)	519, 646, 1613
Eco24I	GRGCYC	1 cut(s)	779
Eco31I	GGTCTC	2 cut(s)	1291, 1966
Eco47I	GGWCC	4 cut(s)	500, 687, 737, 1277
Eco53kI	GAGCTC	1 cut(s)	777
Eco91I	GGTNACC	1 cut(s)	446
EcoICRI	GAGCTC	1 cut(s)	777
EcoO109I	RGGNCCY	2 cut(s)	687, 875
EcoO65I	GGTNACC	1 cut(s)	446
EcoRI	GAATTC	1 cut(s)	1576
EcoRII	CCWGG	6 cut(s)	178, 373, 415, 586, 982, 1282
EcoT14I	CCWWGG	3 cut(s)	519, 646, 1613
EcoT38I	GRGCYC	1 cut(s)	779
ErhI	CCWWGG	3 cut(s)	519, 646, 1613
FalI	AAGNNNNNCTT	2 cut(s)	1023, 1055
FaqI	GGGAC	3 cut(s)	673, 1129, 1829
FauI	CCCGC	3 cut(s)	699, 862, 1693
FauNDI	CATATG	2 cut(s)	1783, 1879
Fnu4HI	GCNGC	5 cut(s)	99, 438, 1142, 1779, 1988
FokI	GGATG	2 cut(s)	941, 1522
FriOI	GRGCYC	1 cut(s)	779
Fsp4HI	GCNGC	5 cut(s)	99, 438, 1142, 1779, 1988
GluI	GCNGC	5 cut(s)	99, 438, 1142, 1779, 1988
GsuI	CTGGAG	2 cut(s)	753, 1452
HaeIII	GGCC	4 cut(s)	480, 859, 876, 1313
HapII	CCGG	5 cut(s)	465, 856, 974, 1098, 1409
HinfI	GANTC	5 cut(s)	231, 336, 421, 655, 1842
HpaII	CCGG	5 cut(s)	465, 856, 974, 1098, 1409
HphI	GGTGA	2 cut(s)	1398, 1649
Hpy166II	GTNNAC	7 cut(s)	62, 263, 503, 740, 1136, 1198, 1277
Hpy188I	TCNGA	3 cut(s)	1006, 1231, 1621
Hpy8I	GTNNAC	7 cut(s)	62, 263, 503, 740, 1136, 1198, 1277
HpyAV	CCTTC	2 cut(s)	158, 231
HpyCH4III	ACNGT	8 cut(s)	452, 643, 815, 1054, 1321, 1386, 1901, 1977
HpyCH4IV	ACGT	1 cut(s)	64
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1072, 1354, 1456
HpyF3I	CTNAG	4 cut(s)	300, 1071, 1228, 1632
HpySE526I	ACGT	1 cut(s)	64
KflI	GGGWCCC	1 cut(s)	687
KpnI	GGTACC	1 cut(s)	587
KroI	GCCGGC	1 cut(s)	855
KroNI	GCCGGC	1 cut(s)	857
Kzo9I	GATC	9 cut(s)	32, 125, 626, 1091, 1207, 1429, 1516, 1639, 1906
LmnI	GCTCC	5 cut(s)	156, 1162, 1471, 1819, 1855
Lsp1109I	GCAGC	4 cut(s)	85, 424, 1153, 1765
LweI	GCATC	3 cut(s)	963, 1053, 1437
MabI	ACCWGGT	1 cut(s)	586
MaeII	ACGT	1 cut(s)	64
MaeIII	GTNAC	4 cut(s)	337, 446, 1380, 1404
MalI	GATC	9 cut(s)	34, 127, 628, 1093, 1209, 1431, 1518, 1641, 1908
MboI	GATC	9 cut(s)	32, 125, 626, 1091, 1207, 1429, 1516, 1639, 1906
MboII	GAAGA	6 cut(s)	12, 38, 275, 977, 1101, 1202
MfeI	CAATTG	3 cut(s)	441, 887, 927
MflI	RGATCY	3 cut(s)	125, 1091, 1207
MhlI	GDGCHC	2 cut(s)	779, 1824
MlyI	GAGTC	3 cut(s)	240, 345, 1851
MmeI	TCCRAC	2 cut(s)	259, 346
MroNI	GCCGGC	1 cut(s)	855
MroXI	GAANNNNTTC	4 cut(s)	253, 969, 1038, 1926
MslI	CAYNNNNRTG	2 cut(s)	1221, 1508
MspI	CCGG	5 cut(s)	465, 856, 974, 1098, 1409
MspR9I	CCNGG	7 cut(s)	180, 375, 417, 465, 588, 984, 1284
MunI	CAATTG	3 cut(s)	441, 887, 927
Mva1269I	GAATGC	1 cut(s)	1249
MvaI	CCWGG	6 cut(s)	180, 375, 417, 588, 984, 1284
MwoI	GCNNNNNNNGC	3 cut(s)	1072, 1354, 1456
NaeI	GCCGGC	1 cut(s)	857
NciI	CCSGG	1 cut(s)	465
NcoI	CCATGG	2 cut(s)	519, 1613
NdeI	CATATG	2 cut(s)	1783, 1879
NdeII	GATC	9 cut(s)	32, 125, 626, 1091, 1207, 1429, 1516, 1639, 1906
NgoMIV	GCCGGC	1 cut(s)	855
NlaIV	GGNNCC	5 cut(s)	585, 688, 689, 1609, 1857
NmuCI	GTSAC	3 cut(s)	337, 1380, 1404
NspI	RCATGY	4 cut(s)	178, 1157, 1753, 1778
NspV	TTCGAA	1 cut(s)	1930
PaeI	GCATGC	1 cut(s)	1753
PagI	TCATGA	1 cut(s)	1042
PctI	GAATGC	1 cut(s)	1249
PdiI	GCCGGC	1 cut(s)	857
PdmI	GAANNNNTTC	4 cut(s)	253, 969, 1038, 1926
PfeI	GAWTC	2 cut(s)	421, 655
PflMI	CCANNNNNTGG	2 cut(s)	487, 731
PfoI	TCCNGGA	1 cut(s)	463
PinAI	ACCGGT	1 cut(s)	1408
PkrI	GCNGC	5 cut(s)	100, 439, 1143, 1780, 1989
PleI	GAGTC	3 cut(s)	239, 344, 1850
PpsI	GAGTC	3 cut(s)	239, 344, 1850
PpuMI	RGGWCCY	1 cut(s)	687
Psp124BI	GAGCTC	1 cut(s)	779
Psp5II	RGGWCCY	1 cut(s)	687
Psp6I	CCWGG	6 cut(s)	178, 373, 415, 586, 982, 1282
PspEI	GGTNACC	1 cut(s)	446
PspGI	CCWGG	6 cut(s)	178, 373, 415, 586, 982, 1282
PspN4I	GGNNCC	5 cut(s)	585, 688, 689, 1609, 1857
PspPI	GGNCC	5 cut(s)	500, 687, 737, 875, 1277
PspPPI	RGGWCCY	1 cut(s)	687
PstNI	CAGNNNCTG	3 cut(s)	98, 1325, 1803
PsuI	RGATCY	3 cut(s)	125, 1091, 1207
RsaI	GTAC	2 cut(s)	184, 585
RsaNI	GTAC	2 cut(s)	183, 584
RseI	CAYNNNNRTG	2 cut(s)	1221, 1508
SacI	GAGCTC	1 cut(s)	779
SatI	GCNGC	5 cut(s)	99, 438, 1142, 1779, 1988
Sau3AI	GATC	9 cut(s)	32, 125, 626, 1091, 1207, 1429, 1516, 1639, 1906
Sau96I	GGNCC	5 cut(s)	500, 687, 737, 875, 1277
SchI	GAGTC	3 cut(s)	240, 345, 1851
ScrFI	CCNGG	7 cut(s)	180, 375, 417, 465, 588, 984, 1284
SduI	GDGCHC	2 cut(s)	779, 1824
SexAI	ACCWGGT	1 cut(s)	586
SfaNI	GCATC	3 cut(s)	963, 1053, 1437
SfuI	TTCGAA	1 cut(s)	1930
SinI	GGWCC	4 cut(s)	500, 687, 737, 1277
SmiMI	CAYNNNNRTG	2 cut(s)	1221, 1508
SmlI	CTYRAG	2 cut(s)	879, 1191
SmoI	CTYRAG	2 cut(s)	879, 1191
SphI	GCATGC	1 cut(s)	1753
SsiI	CCGC	5 cut(s)	405, 692, 869, 1686, 1985
SstI	GAGCTC	1 cut(s)	779
StyD4I	CCNGG	7 cut(s)	178, 373, 415, 463, 586, 982, 1282
StyI	CCWWGG	3 cut(s)	519, 646, 1613
TaaI	ACNGT	8 cut(s)	452, 643, 815, 1054, 1321, 1386, 1901, 1977
TaiI	ACGT	1 cut(s)	67
TaqI	TCGA	2 cut(s)	1445, 1930
TfiI	GAWTC	2 cut(s)	421, 655
TscAI	CASTG	1 cut(s)	1904
TseFI	GTSAC	3 cut(s)	337, 1380, 1404
TseI	GCWGC	5 cut(s)	98, 437, 1141, 1778, 1987
Tsp45I	GTSAC	3 cut(s)	337, 1380, 1404
TspGWI	ACGGA	1 cut(s)	45
TspRI	CASTG	1 cut(s)	1904
Van91I	CCANNNNNTGG	2 cut(s)	487, 731
VpaK11BI	GGWCC	4 cut(s)	500, 687, 737, 1277
XapI	RAATTY	7 cut(s)	483, 607, 715, 802, 1306, 1576, 1939
XceI	RCATGY	4 cut(s)	178, 1157, 1753, 1778
XcmI	CCANNNNNNNNNTGG	2 cut(s)	242, 526
XmnI	GAANNNNTTC	4 cut(s)	253, 969, 1038, 1926

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.