Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0008670

Physical Location & Seq

Genomic Coordinates Forward (+)

69698204 .. 69705080

Gene Structure 6877 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG437100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGCCTGGTACATTAACTTCAAGAAACTCAACATTAACTAATGTTCCTTCTGGTGGAGTCCAACAACCAACTGGAAGCCTTTCTGGTGGACGATTTTCATCAAATAATCTTCCTGTTGCTCTTTCTCAGTTATCTCATGGAAGCTCTCATGGACATTCAGGAGTTACTAATAGAGGAGGTATAAGTGTAGTTGGAAACCCTGGATTTAGTAGTAGCACAAATGGAATTGGCGGTTCTATTCCTGGGATTCTCCCTACCTCTGCTGCAATTGGTAACCGTAATGCTGTTCCCGGATTGGGTGTAGGCCAAATTTTGGGAAATGCAGGTCCACGAATTACAAGTTCCATGGGAAACATGGTTGGTGGGGGCAACATTGGGAGGAGTATTAGTTCTGGTGGAGGCTTGTCGGTGCCTGGTCTTACTTCCCGTCTAAATTTAAGTGGAAATAGTGGATCTGGTAGCTTAACTGTCCAAGGGCAGAATCGCTTGATGGGTGGTGTGCTTCCACAAGGGTCCCCGCAGGTTATTTCAATGTTGGGAAATTCTTATCCAAGTGCTGGAGGTCCACTTTCTCAAAGCCACGTCCAAGTGAATAATTTGAGCTCTATGGGAATGTTGAATGATGTAAATTCTAATGACAGTTCTCCTTTTGACATGAATGATTTCCCTCAATTAACAAGCCGGCCAAGTTCTGCAGGAGGCCCTCAAGGGCAATTGGGTTCATTGCGAAAACAAGGTCTTGGTGTTAGTCCTATTGTTCAACAAAACCAAGAGTTCAGCATCCAAAATGAAGATTTTCCGGCTTTGCCTGGATTTAAAGGTGGTAATTCTGATTATCCAATGGATATGCACCAGAAAGAACAACTCCATGACAATACCGTGTCAATGATGCAATCTCAGCACTTCTCAATGGGAAGATCTGCCGGATTTAACTTGGGAGGGACTTATTCATCTCATCGTCCACAGCAGCAGCAACAACATGCTCCATCAGTCAGCAATAGTGGTGTCTCATTTTCTCAAGTAAACAATCAGGATCTTCTCCATTTGCATGGCTCAGATATGTTCCCATCATCACATTCAACCTATCATTCACAGACAAGTGGACCTCCTGGCATTGGATTGAGACCTTTAAATTCGGCCAATACAGTTTCTGGCATGGGTTCTTATGACCAGCTTATCCAGCAGTATCAACAACACCAAAATCAGTCACAGTTTCGTCTGCAACAGATGTCACCGGTCAATCAATCATTTAGGGATCAGGGAATTAAGTCAATGCAGACCTCGCAATCTGCTCCAGACCCATTTGGTTTGCTTGGTTTGTTAAGTGTTATAAGGATGAGTGATCCTGATTTAACATCTCTGGCACTTGGAATTGATTTGACAACGCTTGGCTTAAACTTGAATTCAACAGAAAATCTGCACAAGACTTTTGGTTCCCCATGGTCTGACGAGCCAGCTAAGGGTGATCCAGAGTTTAGTGTTCCCCAATGTTATTATGCTAAACAACCACCTGCACTACATCAAGGATATTTTTCAAAGTTCTCGGTGGAAACATTATTTTACATATTTTACAGCATGCCTAAAGATGAAGCTCAATTACATGCTGCAAATGAACTTTATAACAAGGGCTGGTTATATCACAAGGAGCACCATCTATGGATTGCAAGAGTCCCCAACATGGAGCCACTTGTCAAGACAAACACATATGAGAGAGGGTCTTATCACTGTTTTGATCCCAACACATTTGAAATAGTTCGAAAGGAAAATTTTGTTGTGCATTATGATGTGCTGGATAAGAGACCGTCCTTACCGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000288 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006355 GO:0006401 GO:0006402 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0015074 GO:0016070 GO:0016071 GO:0017148 GO:0019219 GO:0019222 GO:0019439 GO:0030014 GO:0030015 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032268 GO:0032269 GO:0032991 GO:0034248 GO:0034249 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1903506 GO:1990904 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

NOT2_3_5

Protein Analysis

605

Amino Acids

64.62

Weight (kDa)

6.91

Isoelectric Point (pI)

52.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NOT2_3_5_C	PF04153	474 - 597	4.9e-40	NOT2/NOT3/NOT5 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008670)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07705 AT1G07705
fragaria_vesca	FvH4_3g34620 FvH4_3g34620 FvH4_3g34620
malus_domestica	MD03G1103300.v1.1 MD11G1117100.v1.1
prunus_persica	Prupe.6G087300_v2.0.a1
pyrus_communis	pycom03g08390 pycom11g09830
rosa_chinensis	RchiOBHm_Chr5g0062331
rosa_laevigata	RLG00000035550
rosa_multiflora	Rmu_sc0006295.1_g000002 Rmu_ssc0000409.1_g000034
rosa_roxburghii	Rroxscaffold_1G00018350
rosa_rugosa	Rorug05G0348500 Rorug05G0348600 Rorug05G0348700
rosa_samantha	Rh5AG408400 Rh5BG422100 Rh5CG446100 Rh5DG437100
rosa_wichuraiana	Rw5G038480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1331, 1620
AarI	CACCTGC	1 cut(s)	1519
Acc36I	ACCTGC	3 cut(s)	314, 511, 1519
AccB1I	GGYRCC	1 cut(s)	409
AccB7I	CCANNNNNTGG	2 cut(s)	313, 557
AciI	CCGC	3 cut(s)	231, 518, 1811
AclWI	GGATC	6 cut(s)	460, 1041, 1263, 1337, 1460, 1727
AcoI	YGGCCR	2 cut(s)	683, 1137
AcsI	RAATTY	7 cut(s)	309, 433, 541, 628, 1132, 1402, 1765
AfaI	GTAC	1 cut(s)	10
AfiI	CCNNNNNNNGG	7 cut(s)	53, 296, 313, 557, 1115, 1460, 1678
AgeI	ACCGGT	1 cut(s)	1234
AgsI	TTSAA	9 cut(s)	21, 531, 619, 761, 1080, 1402, 1407, 1536, 1748
AjiI	CACGTC	1 cut(s)	583
AjnI	CCWGG	6 cut(s)	4, 199, 241, 412, 808, 1108
AloI	GAACNNNNNNTCC	2 cut(s)	373, 405
AluBI	AGCT	6 cut(s)	144, 462, 603, 1174, 1457, 1592
AluI	AGCT	6 cut(s)	144, 462, 603, 1174, 1457, 1592
Alw21I	GWGCWC	2 cut(s)	605, 1650
Alw26I	GTCTC	3 cut(s)	1012, 1117, 1792
AlwI	GGATC	6 cut(s)	460, 1041, 1263, 1337, 1460, 1727
AlwNI	CAGNNNCTG	1 cut(s)	1151
AoxI	GGCC	4 cut(s)	304, 683, 700, 1137
ApeKI	GCWGC	4 cut(s)	263, 967, 970, 1604
ApoI	RAATTY	7 cut(s)	309, 433, 541, 628, 1132, 1402, 1765
ArsI	GACNNNNNNTTYG	2 cut(s)	721, 753
AsiGI	ACCGGT	1 cut(s)	1234
Asp700I	GAANNNNTTC	4 cut(s)	79, 662, 795, 1752
AspS9I	GGNCC	5 cut(s)	326, 513, 563, 701, 1103
AsuC2I	CCSGG	1 cut(s)	291
AsuHPI	GGTGA	2 cut(s)	1224, 1475
AsuII	TTCGAA	1 cut(s)	1756
AvaII	GGWCC	4 cut(s)	326, 513, 563, 1103
BanI	GGYRCC	1 cut(s)	409
BanII	GRGCYC	1 cut(s)	605
BarI	GAAGNNNNNNTAC	3 cut(s)	33, 1581, 1613
Bbv12I	GWGCWC	2 cut(s)	605, 1650
BbvI	GCAGC	4 cut(s)	250, 979, 982, 1591
BccI	CCATC	4 cut(s)	484, 994, 1075, 1659
BciT130I	CCWGG	6 cut(s)	6, 201, 243, 414, 810, 1110
BcnI	CCSGG	1 cut(s)	291
BcoDI	GTCTC	3 cut(s)	1012, 1117, 1792
BfmI	CTRYAG	1 cut(s)	693
BfuAI	ACCTGC	3 cut(s)	314, 511, 1519
BglII	AGATCT	1 cut(s)	917
BisI	GCNGC	4 cut(s)	264, 968, 971, 1605
BlsI	GCNGC	4 cut(s)	265, 969, 972, 1606
Bme1390I	CCNGG	7 cut(s)	6, 201, 243, 291, 414, 810, 1110
Bme18I	GGWCC	4 cut(s)	326, 513, 563, 1103
BmgBI	CACGTC	1 cut(s)	583
BmgT120I	GGNCC	5 cut(s)	326, 513, 563, 701, 1103
BmiI	GGNNCC	5 cut(s)	411, 514, 515, 1435, 1683
BmrFI	CCNGG	7 cut(s)	6, 201, 243, 291, 414, 810, 1110
BmsI	GCATC	2 cut(s)	789, 879
BpmI	CTGGAG	2 cut(s)	579, 1278
Bpu10I	CCTNAGC	1 cut(s)	1458
Bpu14I	TTCGAA	1 cut(s)	1756
BpuEI	CTTGAG	2 cut(s)	690, 1002
BpuMI	CCSGG	1 cut(s)	291
BsaBI	GATNNNNATC	1 cut(s)	97
BsaI	GGTCTC	2 cut(s)	1117, 1792
BsaJI	CCNNGG	5 cut(s)	199, 242, 345, 472, 1439
BsaWI	WCCGGW	1 cut(s)	1234
BsaXI	ACNNNNNCTCC	2 cut(s)	373, 403
Bsc4I	CCNNNNNNNGG	7 cut(s)	53, 296, 313, 557, 1115, 1460, 1678
Bse118I	RCCGGY	2 cut(s)	681, 1234
Bse1I	ACTGG	1 cut(s)	76
Bse3DI	GCAATG	1 cut(s)	722
Bse8I	GATNNNNATC	1 cut(s)	97
BseBI	CCWGG	6 cut(s)	6, 201, 243, 414, 810, 1110
BseDI	CCNNGG	5 cut(s)	199, 242, 345, 472, 1439
BseGI	GGATG	2 cut(s)	780, 1341
BseJI	GATNNNNATC	1 cut(s)	97
BseLI	CCNNNNNNNGG	7 cut(s)	53, 296, 313, 557, 1115, 1460, 1678
BseMI	GCAATG	1 cut(s)	722
BseMII	CTCAG	3 cut(s)	140, 911, 1068
BseNI	ACTGG	1 cut(s)	76
BseRI	GAGGAG	2 cut(s)	189, 394
BseXI	GCAGC	4 cut(s)	250, 979, 982, 1591
BsgI	GTGCAG	2 cut(s)	1403, 1497
BshFI	GGCC	4 cut(s)	306, 685, 702, 1139
BshNI	GGYRCC	1 cut(s)	409
BshTI	ACCGGT	1 cut(s)	1234
BsiHKAI	GWGCWC	2 cut(s)	605, 1650
BsiSI	CCGG	5 cut(s)	291, 682, 800, 924, 1235
BslFI	GGGAC	3 cut(s)	499, 955, 1655
BslI	CCNNNNNNNGG	7 cut(s)	53, 296, 313, 557, 1115, 1460, 1678
BsmAI	GTCTC	3 cut(s)	1012, 1117, 1792
BsmFI	GGGAC	3 cut(s)	499, 955, 1655
BsnI	GGCC	4 cut(s)	306, 685, 702, 1139
Bso31I	GGTCTC	2 cut(s)	1117, 1792
Bsp119I	TTCGAA	1 cut(s)	1756
Bsp1286I	GDGCHC	2 cut(s)	605, 1650
Bsp143I	GATC	7 cut(s)	452, 917, 1033, 1255, 1342, 1465, 1732
Bsp19I	CCATGG	2 cut(s)	345, 1439
BspACI	CCGC	3 cut(s)	231, 518, 1811
BspANI	GGCC	4 cut(s)	306, 685, 702, 1139
BspCNI	CTCAG	3 cut(s)	139, 910, 1067
BspLI	GGNNCC	5 cut(s)	411, 514, 515, 1435, 1683
BspMAI	CTGCAG	1 cut(s)	697
BspMI	ACCTGC	3 cut(s)	314, 511, 1519
BspPI	GGATC	6 cut(s)	460, 1041, 1263, 1337, 1460, 1727
BspT104I	TTCGAA	1 cut(s)	1756
BspT107I	GGYRCC	1 cut(s)	409
BspTNI	GGTCTC	2 cut(s)	1117, 1792
BsrDI	GCAATG	1 cut(s)	722
BsrFI	RCCGGY	2 cut(s)	681, 1234
BsrI	ACTGG	1 cut(s)	76
BssAI	RCCGGY	2 cut(s)	681, 1234
BssECI	CCNNGG	5 cut(s)	199, 242, 345, 472, 1439
BssMI	GATC	7 cut(s)	452, 917, 1033, 1255, 1342, 1465, 1732
BssT1I	CCWWGG	3 cut(s)	345, 472, 1439
Bst2UI	CCWGG	6 cut(s)	6, 201, 243, 414, 810, 1110
Bst4CI	ACNGT	8 cut(s)	278, 469, 641, 880, 1147, 1212, 1727, 1803
BstBI	TTCGAA	1 cut(s)	1756
BstC8I	GCNNGC	3 cut(s)	683, 1455, 1577
BstDEI	CTNAG	4 cut(s)	126, 897, 1054, 1458
BstDSI	CCRYGG	2 cut(s)	345, 1439
BstEII	GGTNACC	1 cut(s)	272
BstF5I	GGATG	2 cut(s)	780, 1341
BstKTI	GATC	7 cut(s)	455, 920, 1036, 1258, 1345, 1468, 1735
BstMAI	GTCTC	3 cut(s)	1012, 1117, 1792
BstMBI	GATC	7 cut(s)	452, 917, 1033, 1255, 1342, 1465, 1732
BstMWI	GCNNNNNNNGC	3 cut(s)	898, 1180, 1282
BstNI	CCWGG	6 cut(s)	6, 201, 243, 414, 810, 1110
BstNSI	RCATGY	3 cut(s)	983, 1579, 1604
BstPI	GGTNACC	1 cut(s)	272
BstSCI	CCNGG	7 cut(s)	4, 199, 241, 289, 412, 808, 1108
BstSFI	CTRYAG	1 cut(s)	693
BstV1I	GCAGC	4 cut(s)	250, 979, 982, 1591
BstX2I	RGATCY	3 cut(s)	452, 917, 1033
BstXI	CCANNNNNNTGG	1 cut(s)	1049
BstYI	RGATCY	3 cut(s)	452, 917, 1033
BsuRI	GGCC	4 cut(s)	306, 685, 702, 1139
BtgI	CCRYGG	2 cut(s)	345, 1439
BtrI	CACGTC	1 cut(s)	583
BtsCI	GGATG	2 cut(s)	780, 1341
BtsIMutI	CAGTG	1 cut(s)	1723
BveI	ACCTGC	3 cut(s)	314, 511, 1519
Cac8I	GCNNGC	3 cut(s)	683, 1455, 1577
CaiI	CAGNNNCTG	1 cut(s)	1151
Cfr10I	RCCGGY	2 cut(s)	681, 1234
Cfr13I	GGNCC	5 cut(s)	326, 513, 563, 701, 1103
Csp6I	GTAC	1 cut(s)	9
CspAI	ACCGGT	1 cut(s)	1234
CviQI	GTAC	1 cut(s)	9
DdeI	CTNAG	4 cut(s)	126, 897, 1054, 1458
DpnI	GATC	7 cut(s)	454, 919, 1035, 1257, 1344, 1467, 1734
DpnII	GATC	7 cut(s)	452, 917, 1033, 1255, 1342, 1465, 1732
DraI	TTTAAA	2 cut(s)	817, 1131
EaeI	YGGCCR	2 cut(s)	683, 1137
Ecl136II	GAGCTC	1 cut(s)	603
Eco130I	CCWWGG	3 cut(s)	345, 472, 1439
Eco24I	GRGCYC	1 cut(s)	605
Eco31I	GGTCTC	2 cut(s)	1117, 1792
Eco47I	GGWCC	4 cut(s)	326, 513, 563, 1103
Eco53kI	GAGCTC	1 cut(s)	603
Eco91I	GGTNACC	1 cut(s)	272
EcoICRI	GAGCTC	1 cut(s)	603
EcoO109I	RGGNCCY	2 cut(s)	513, 701
EcoO65I	GGTNACC	1 cut(s)	272
EcoRI	GAATTC	1 cut(s)	1402
EcoRII	CCWGG	6 cut(s)	4, 199, 241, 412, 808, 1108
EcoT14I	CCWWGG	3 cut(s)	345, 472, 1439
EcoT38I	GRGCYC	1 cut(s)	605
ErhI	CCWWGG	3 cut(s)	345, 472, 1439
FaqI	GGGAC	3 cut(s)	499, 955, 1655
FauI	CCCGC	1 cut(s)	525
FauNDI	CATATG	1 cut(s)	1705
Fnu4HI	GCNGC	4 cut(s)	264, 968, 971, 1605
FokI	GGATG	2 cut(s)	767, 1348
FriOI	GRGCYC	1 cut(s)	605
Fsp4HI	GCNGC	4 cut(s)	264, 968, 971, 1605
GluI	GCNGC	4 cut(s)	264, 968, 971, 1605
GsuI	CTGGAG	2 cut(s)	579, 1278
HaeIII	GGCC	4 cut(s)	306, 685, 702, 1139
HapII	CCGG	5 cut(s)	291, 682, 800, 924, 1235
HinfI	GANTC	4 cut(s)	57, 247, 481, 1668
HpaII	CCGG	5 cut(s)	291, 682, 800, 924, 1235
HphI	GGTGA	2 cut(s)	1224, 1475
Hpy166II	GTNNAC	6 cut(s)	89, 329, 566, 962, 1024, 1103
Hpy188I	TCNGA	3 cut(s)	832, 1057, 1447
Hpy188III	TCNNGA	7 cut(s)	21, 159, 1031, 1295, 1346, 1469, 1693
Hpy8I	GTNNAC	6 cut(s)	89, 329, 566, 962, 1024, 1103
HpyAV	CCTTC	1 cut(s)	57
HpyCH4III	ACNGT	8 cut(s)	278, 469, 641, 880, 1147, 1212, 1727, 1803
HpyCH4IV	ACGT	1 cut(s)	582
HpyF10VI	GCNNNNNNNGC	3 cut(s)	898, 1180, 1282
HpyF3I	CTNAG	4 cut(s)	126, 897, 1054, 1458
HpySE526I	ACGT	1 cut(s)	582
KflI	GGGWCCC	1 cut(s)	513
KroI	GCCGGC	1 cut(s)	681
KroNI	GCCGGC	1 cut(s)	683
Kzo9I	GATC	7 cut(s)	452, 917, 1033, 1255, 1342, 1465, 1732
LmnI	GCTCC	4 cut(s)	988, 1297, 1645, 1681
Lsp1109I	GCAGC	4 cut(s)	250, 979, 982, 1591
LweI	GCATC	2 cut(s)	789, 879
MaeII	ACGT	1 cut(s)	582
MaeIII	GTNAC	4 cut(s)	163, 272, 1206, 1230
MalI	GATC	7 cut(s)	454, 919, 1035, 1257, 1344, 1467, 1734
MboI	GATC	7 cut(s)	452, 917, 1033, 1255, 1342, 1465, 1732
MboII	GAAGA	4 cut(s)	101, 803, 927, 1028
MfeI	CAATTG	2 cut(s)	267, 713
MflI	RGATCY	3 cut(s)	452, 917, 1033
MhlI	GDGCHC	2 cut(s)	605, 1650
MlyI	GAGTC	2 cut(s)	66, 1677
MmeI	TCCRAC	2 cut(s)	85, 172
MroNI	GCCGGC	1 cut(s)	681
MroXI	GAANNNNTTC	4 cut(s)	79, 662, 795, 1752
MslI	CAYNNNNRTG	2 cut(s)	1047, 1782
MspI	CCGG	5 cut(s)	291, 682, 800, 924, 1235
MspR9I	CCNGG	7 cut(s)	6, 201, 243, 291, 414, 810, 1110
MunI	CAATTG	2 cut(s)	267, 713
MvaI	CCWGG	6 cut(s)	6, 201, 243, 414, 810, 1110
MwoI	GCNNNNNNNGC	3 cut(s)	898, 1180, 1282
NaeI	GCCGGC	1 cut(s)	683
NciI	CCSGG	1 cut(s)	291
NcoI	CCATGG	2 cut(s)	345, 1439
NdeI	CATATG	1 cut(s)	1705
NdeII	GATC	7 cut(s)	452, 917, 1033, 1255, 1342, 1465, 1732
NgoMIV	GCCGGC	1 cut(s)	681
NlaIV	GGNNCC	5 cut(s)	411, 514, 515, 1435, 1683
NmuCI	GTSAC	2 cut(s)	1206, 1230
NspI	RCATGY	3 cut(s)	983, 1579, 1604
NspV	TTCGAA	1 cut(s)	1756
PaeI	GCATGC	1 cut(s)	1579
PaqCI	CACCTGC	1 cut(s)	1519
PdiI	GCCGGC	1 cut(s)	683
PdmI	GAANNNNTTC	4 cut(s)	79, 662, 795, 1752
PfeI	GAWTC	2 cut(s)	247, 481
PflMI	CCANNNNNTGG	2 cut(s)	313, 557
PfoI	TCCNGGA	1 cut(s)	289
PinAI	ACCGGT	1 cut(s)	1234
PkrI	GCNGC	4 cut(s)	265, 969, 972, 1606
PleI	GAGTC	2 cut(s)	65, 1676
PpsI	GAGTC	2 cut(s)	65, 1676
PpuMI	RGGWCCY	1 cut(s)	513
PsiI	TTATAA	2 cut(s)	1331, 1620
Psp124BI	GAGCTC	1 cut(s)	605
Psp5II	RGGWCCY	1 cut(s)	513
Psp6I	CCWGG	6 cut(s)	4, 199, 241, 412, 808, 1108
PspEI	GGTNACC	1 cut(s)	272
PspGI	CCWGG	6 cut(s)	4, 199, 241, 412, 808, 1108
PspN4I	GGNNCC	5 cut(s)	411, 514, 515, 1435, 1683
PspPI	GGNCC	5 cut(s)	326, 513, 563, 701, 1103
PspPPI	RGGWCCY	1 cut(s)	513
PstI	CTGCAG	1 cut(s)	697
PstNI	CAGNNNCTG	1 cut(s)	1151
PsuI	RGATCY	3 cut(s)	452, 917, 1033
RsaI	GTAC	1 cut(s)	10
RsaNI	GTAC	1 cut(s)	9
RseI	CAYNNNNRTG	2 cut(s)	1047, 1782
SacI	GAGCTC	1 cut(s)	605
SatI	GCNGC	4 cut(s)	264, 968, 971, 1605
Sau3AI	GATC	7 cut(s)	452, 917, 1033, 1255, 1342, 1465, 1732
Sau96I	GGNCC	5 cut(s)	326, 513, 563, 701, 1103
SchI	GAGTC	2 cut(s)	66, 1677
ScrFI	CCNGG	7 cut(s)	6, 201, 243, 291, 414, 810, 1110
SduI	GDGCHC	2 cut(s)	605, 1650
SfaNI	GCATC	2 cut(s)	789, 879
SfcI	CTRYAG	1 cut(s)	693
SfuI	TTCGAA	1 cut(s)	1756
SinI	GGWCC	4 cut(s)	326, 513, 563, 1103
SmiMI	CAYNNNNRTG	2 cut(s)	1047, 1782
SmlI	CTYRAG	2 cut(s)	705, 1017
SmoI	CTYRAG	2 cut(s)	705, 1017
SphI	GCATGC	1 cut(s)	1579
SsiI	CCGC	3 cut(s)	231, 518, 1811
SstI	GAGCTC	1 cut(s)	605
StyD4I	CCNGG	7 cut(s)	4, 199, 241, 289, 412, 808, 1108
StyI	CCWWGG	3 cut(s)	345, 472, 1439
TaaI	ACNGT	8 cut(s)	278, 469, 641, 880, 1147, 1212, 1727, 1803
TaiI	ACGT	1 cut(s)	585
TaqI	TCGA	1 cut(s)	1756
TfiI	GAWTC	2 cut(s)	247, 481
TscAI	CASTG	1 cut(s)	1730
TseFI	GTSAC	2 cut(s)	1206, 1230
TseI	GCWGC	4 cut(s)	263, 967, 970, 1604
Tsp45I	GTSAC	2 cut(s)	1206, 1230
TspDTI	ATGAA	7 cut(s)	87, 671, 711, 804, 939, 1602, 1626
TspRI	CASTG	1 cut(s)	1730
Van91I	CCANNNNNTGG	2 cut(s)	313, 557
VpaK11BI	GGWCC	4 cut(s)	326, 513, 563, 1103
XapI	RAATTY	7 cut(s)	309, 433, 541, 628, 1132, 1402, 1765
XceI	RCATGY	3 cut(s)	983, 1579, 1604
XcmI	CCANNNNNNNNNTGG	2 cut(s)	68, 352
XmnI	GAANNNNTTC	4 cut(s)	79, 662, 795, 1752

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.