Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010089

Physical Location & Seq

Genomic Coordinates Reverse (-)

12188360 .. 12196972

Gene Structure 8613 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1127000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2400 bp

ATGCTCTCACGATCACTCTGTTCATGCCATTGTCTACTTCTCCCCTCTCTCCGCCGTCTCCACACCCCCATCTCCCTCTTCCGCCCTAAACCCTCTTCTCTTCCTCTCATTTCCCTCTTACCCCAAAACCCTAATCTTCGCGCCATGTCCTCCCGCCCCTCCGCCTTCGACGCCCTCATGTCCGGCGCCCGCGCCGCCGCCGCCAAGAAAAAGCCCCAGCCTTCTCCCTCCAAGAAGCGCAAAGCCCTAGACTCCACTTACCCTATCAAAAAACCCCAATTGCTGCCTACGCCCGAAAGCGCCTGCGCAACCCAAGACCCAGAATCCGTGGAAGTCAAAGCTCCGGAATCCAAGGAAATTAAAGAAGCTCAAGAAAGTTCTGGCAATGGCGGTGAGGTGGTTCCGGCAGGGAAGACAGTGTGCGTGGCGGCGGGTGCTAAGGAGCGGACTGAGGAATTGAAGAGCCGAATGCCGCTACTGAAGAAGAAAGCCGGGGAATTTGACCCAAAATTGGTGGCGAATTGGGGCCAGGGCGAGAGGGTTCCTTTTGTGTTTGTTTGTTTAGTTTTCGATTTGATTGACAAAGAGACGGGTCGAATTGTGATCACGGATATTGTTTGTAACATGTTGAGGACTGTAATGTACACCACGCCTGAGGATTTGGTGCCTGTGATTTATCTTGCGGCGAATAGGATTGCTCCTGCGCATGAAGGTTTGGAGCTTGGAATTGGGGATGCTTCGATTGTTAAGGCCCTTGCAGAGGCTTGTGGGAGGACTGAAGCGCATGTGAAGAAGCAATACAAGGAGCTGGGAGATCTTGGTCATGTTGCAAAGGAAAGCCGTTCTTCTCAATCTATGATGCGCAAGCCTGATGCATTAACTGTCACCAAGGTTTTTAGTACATTTCGTCTACTAGCTAAGGGACCTGGTGAATCTGGGAAGGATAGTCAGGAGAAGAAAAAGAACCATATCAAGGCACTTCTTGTTGCTGCCACTGACTGTGAACCTCTATATCTGATCCGTTTGCTTCAGACAAAGTTGCGAATTGGATTGGCAGAGCAGACTCTATTAACTGCATTGGGACAAGCTGCAGTGTATACTGAAAAACATTCGACACCACCTTCTCATATTCAGAGTCCTCTAGAAGAGGCAGCAAAGATTGTTAAGCAAGTGTACTCTCTGCTTCCTGATTACGATAAAATCATCCCTGCCCTTTTAAGTGGAGGTGTATGGAATCTTCCAGAGATATGCAACTTTACTTTGGGCGTCCCAGTTGGGCCTATGCTAGCAAAACCAACTAAAGGGGTATCTGAGATTGTACAGAAGTTTCAGGATACAGAATACACGTGTGAATACAAGTACGATGGAGAACGTGCCCAGATACATTTCATGGAGAATGGTTCTGTTGAGATATACAGTCGAAATGCTGAACGAAACACTGGAAAGTATCCTGATGTGGTTGCTGCAGTGTCGAGGTTAAAGAAGTCATCTGTAACATCATTCGTTTTGGATTGTGAAATTGTTGCTTATGACCGTGAAAAACAAAAAATTCTGCCCTTCCAGGTACTCAGCACTAGAGCGCGTAAAAATGTGAGCGTGAGTGAGATCAAGGTTGAAGTCTGCATATTTGCTTTTGATATGTTGTACCTTAATGGCCAACCACTTATTCAGGAACAACTGAAAGTTCGTAGAGAGTGTCTGTATGACTCTTTTGTGGAAGAAGCTGGGTTTTTTCAGTTTGCCACAGCCTTAACATCAAATGATATTGATGAAATACAAAAGTTTCTTGATGCTGCTGTCAGTTCAAGTTCTGAGGGTTTAATTATTAAAACATTGACCAAAGATGCTACTTACGAGCCTTCAAAGCGGTCACTTAACTGGCTGAAATTGAAGAAAGATTACATGGACAGTATTGGGGACTCACTAGACTTGGTACCTATTGCTGCTTTCCATGGCCGGGGAAAACGTACAGGTGTCTATGGTGCTTTCCTCCTCGCTTGTTATGATAGCAATAATGAAGAGTTTCAAAGCATTTGTAAAGTAGGCACTGGACTCACTGAAGCAATGCTTGAAGAACGTTCTGCCAGTCTCCGTACTAAAGTGATACCCAAACCTAAGTCATACTATAGGTATCATGATACAATGAATCCAGATGTATGGTTTGAACCCTCAGAGGTTTGGGAGGTGAAAGCTGCGGACCTGACTATTAGCCCCGTTCATCGTGCTGCAGTTGGCATAGTGGATGCTAATAAGGGTATTTCTCTCCGGTTTCCACGTCTTATTCGAGTTCGGGAAGACAAACCTCCGGAGCAAGCCACATCATCTGAGCAGGTAGCCGAGTTGTATAATGCTCAAGCGATCAACCATTCAAACAATCAAAATGACAACGAAGATGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000012 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016874 GO:0016886 GO:0022616 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0140097 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430

Pfam Domains

Protein Families

DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N

Protein Analysis

800

Amino Acids

88.43

Weight (kDa)

8.37

Isoelectric Point (pI)

47.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_ligase_A_N	PF04675	182 - 360	1.6e-48	DNA ligase N terminus
DNA_ligase_A_M	PF01068	427 - 631	1.3e-68	ATP dependent DNA ligase domain
DNA_ligase_A_C	PF04679	656 - 767	4.5e-22	ATP dependent DNA ligase C terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08130
fragaria_vesca	FvH4_3g30730 FvH4_3g30731 FvH4_3g30740
malus_domestica	MD03G1127000.v1.1 MD11G1147600.v1.1
prunus_persica	Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0056931
rosa_laevigata	RLG00000035140
rosa_multiflora	Rmu_sc0002035.1_g000008
rosa_roxburghii	Rroxscaffold_1G00023260 Rroxscaffold_1G00059860
rosa_rugosa	Rorug05G0304400
rosa_samantha	Rh5AG373800 Rh5BG384800 Rh5CG408100
rosa_wichuraiana	Rw5G035180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	3 cut(s)	307, 705, 863
Acc36I	ACCTGC	1 cut(s)	2320
Acc65I	GGTACC	1 cut(s)	1933
AccB1I	GGYRCC	3 cut(s)	185, 664, 1933
AccBSI	CCGCTC	1 cut(s)	445
AccI	GTMKAC	3 cut(s)	34, 910, 1097
AccII	CGCG	3 cut(s)	141, 192, 1582
AccIII	TCCGGA	2 cut(s)	343, 2305
AclI	AACGTT	1 cut(s)	2077
AclWI	GGATC	1 cut(s)	1012
AcoI	YGGCCR	2 cut(s)	1654, 1954
AcsI	RAATTY	2 cut(s)	497, 1548
AcuI	CTGAAG	4 cut(s)	500, 798, 1013, 2079
AcvI	CACGTG	1 cut(s)	1347
AcyI	GRCGYC	3 cut(s)	171, 186, 1266
AfiI	CCNNNNNNNGG	5 cut(s)	511, 760, 973, 1301, 1957
AflIII	ACRYGT	3 cut(s)	624, 1344, 1346
AgsI	TTSAA	9 cut(s)	460, 1616, 1806, 1863, 1891, 2027, 2072, 2165, 2370
AjiI	CACGTC	1 cut(s)	2276
AjnI	CCWGG	3 cut(s)	528, 925, 1560
AjuI	GAANNNNNNNTTGG	2 cut(s)	1650, 1682
AluBI	AGCT	8 cut(s)	341, 368, 721, 808, 917, 1088, 1724, 2192
AluI	AGCT	8 cut(s)	341, 368, 721, 808, 917, 1088, 1724, 2192
Alw26I	GTCTC	3 cut(s)	62, 581, 2093
AlwI	GGATC	1 cut(s)	1012
Aor13HI	TCCGGA	2 cut(s)	343, 2305
AoxI	GGCC	5 cut(s)	526, 750, 1277, 1654, 1954
ApeKI	GCWGC	9 cut(s)	283, 989, 1088, 1151, 1463, 1793, 1943, 2192, 2225
ApoI	RAATTY	2 cut(s)	497, 1548
Asp700I	GAANNNNTTC	1 cut(s)	2022
Asp718I	GGTACC	1 cut(s)	1933
AspS9I	GGNCC	5 cut(s)	526, 751, 923, 1277, 2197
AsuC2I	CCSGG	2 cut(s)	493, 1958
AsuHPI	GGTGA	4 cut(s)	404, 877, 941, 2197
AsuNHI	GCTAGC	1 cut(s)	1285
AvaII	GGWCC	2 cut(s)	923, 2197
AxyI	CCTNAGG	1 cut(s)	654
BaeGI	GKGCMC	1 cut(s)	1378
BalI	TGGCCA	1 cut(s)	1656
BanI	GGYRCC	3 cut(s)	185, 664, 1933
BarI	GAAGNNNNNNTAC	6 cut(s)	782, 814, 1221, 1253, 1883, 1915
BbrPI	CACGTG	1 cut(s)	1347
BbsI	GAAGAC	2 cut(s)	419, 2301
BbvI	GCAGC	9 cut(s)	270, 976, 1075, 1163, 1450, 1780, 1930, 2179, 2212
BccI	CCATC	2 cut(s)	77, 1358
BceAI	ACGGC	2 cut(s)	39, 825
BciT130I	CCWGG	3 cut(s)	530, 927, 1562
BciVI	GTATCC	2 cut(s)	1327, 1458
BclI	TGATCA	1 cut(s)	603
BcnI	CCSGG	2 cut(s)	493, 1958
BcoDI	GTCTC	3 cut(s)	62, 581, 2093
BfaI	CTAG	6 cut(s)	248, 914, 1142, 1286, 1575, 1925
BfmI	CTRYAG	4 cut(s)	1089, 1464, 2125, 2226
BfoI	RGCGCY	2 cut(s)	189, 303
BfuAI	ACCTGC	1 cut(s)	2320
BfuI	GTATCC	2 cut(s)	1327, 1458
BglII	AGATCT	1 cut(s)	814
Bme1390I	CCNGG	5 cut(s)	493, 530, 927, 1562, 1958
Bme18I	GGWCC	2 cut(s)	923, 2197
BmgBI	CACGTC	1 cut(s)	2276
BmgT120I	GGNCC	5 cut(s)	526, 751, 923, 1277, 2197
BmiI	GGNNCC	7 cut(s)	187, 402, 527, 543, 666, 924, 1935
BmrFI	CCNGG	5 cut(s)	493, 530, 927, 1562, 1958
BmrI	ACTGGG	1 cut(s)	1265
BmsI	GCATC	6 cut(s)	724, 849, 862, 1780, 1834, 2233
BmtI	GCTAGC	1 cut(s)	1289
BmuI	ACTGGG	1 cut(s)	1265
BpiI	GAAGAC	2 cut(s)	419, 2301
Bpu10I	CCTNAGC	2 cut(s)	438, 918
BpuEI	CTTGAG	2 cut(s)	354, 2337
BpuMI	CCSGG	2 cut(s)	493, 1958
BsaAI	YACGTR	1 cut(s)	1347
BsaHI	GRCGYC	3 cut(s)	171, 186, 1266
BsaJI	CCNNGG	7 cut(s)	327, 351, 492, 529, 888, 1951, 1957
BsaWI	WCCGGW	3 cut(s)	343, 2265, 2305
Bsc4I	CCNNNNNNNGG	5 cut(s)	511, 760, 973, 1301, 1957
Bse1I	ACTGG	5 cut(s)	1271, 1444, 1883, 2053, 2085
Bse21I	CCTNAGG	1 cut(s)	654
Bse3DI	GCAATG	2 cut(s)	391, 2070
BseAI	TCCGGA	2 cut(s)	343, 2305
BseBI	CCWGG	3 cut(s)	530, 927, 1562
BseDI	CCNNGG	7 cut(s)	327, 351, 492, 529, 888, 1951, 1957
BseGI	GGATG	3 cut(s)	739, 1203, 2248
BseLI	CCNNNNNNNGG	5 cut(s)	511, 760, 973, 1301, 1957
BseMI	GCAATG	2 cut(s)	391, 2070
BseMII	CTCAG	7 cut(s)	441, 645, 1302, 1582, 1803, 2184, 2316
BseNI	ACTGG	5 cut(s)	1271, 1444, 1883, 2053, 2085
BseRI	GAGGAG	1 cut(s)	1982
BseSI	GKGCMC	1 cut(s)	1378
BseXI	GCAGC	9 cut(s)	270, 976, 1075, 1163, 1450, 1780, 1930, 2179, 2212
BseYI	CCCAGC	3 cut(s)	216, 808, 1724
Bsh1236I	CGCG	3 cut(s)	141, 192, 1582
BshFI	GGCC	5 cut(s)	528, 752, 1279, 1656, 1956
BshNI	GGYRCC	3 cut(s)	185, 664, 1933
BsiSI	CCGG	7 cut(s)	183, 344, 404, 492, 1957, 2266, 2306
BslFI	GGGAC	4 cut(s)	936, 1095, 1253, 1931
BslI	CCNNNNNNNGG	5 cut(s)	511, 760, 973, 1301, 1957
BsmAI	GTCTC	3 cut(s)	62, 581, 2093
BsmBI	CGTCTC	2 cut(s)	62, 581
BsmFI	GGGAC	4 cut(s)	936, 1095, 1253, 1931
BsmI	GAATGC	1 cut(s)	474
BsnI	GGCC	5 cut(s)	528, 752, 1279, 1656, 1956
Bsp1286I	GDGCHC	1 cut(s)	1378
Bsp13I	TCCGGA	2 cut(s)	343, 2305
Bsp1407I	TGTACA	2 cut(s)	642, 1318
Bsp143I	GATC	6 cut(s)	11, 603, 814, 1017, 1605, 2358
Bsp19I	CCATGG	1 cut(s)	1951
BspANI	GGCC	5 cut(s)	528, 752, 1279, 1656, 1956
BspCNI	CTCAG	7 cut(s)	442, 646, 1303, 1581, 1804, 2183, 2317
BspEI	TCCGGA	2 cut(s)	343, 2305
BspFNI	CGCG	3 cut(s)	141, 192, 1582
BspHI	TCATGA	1 cut(s)	2134
BspLI	GGNNCC	7 cut(s)	187, 402, 527, 543, 666, 924, 1935
BspMAI	CTGCAG	3 cut(s)	1093, 1468, 2230
BspMI	ACCTGC	1 cut(s)	2320
BspOI	GCTAGC	1 cut(s)	1289
BspPI	GGATC	1 cut(s)	1012
BspQI	GCTCTTC	1 cut(s)	455
BspT107I	GGYRCC	3 cut(s)	185, 664, 1933
BsrBI	CCGCTC	1 cut(s)	445
BsrDI	GCAATG	2 cut(s)	391, 2070
BsrGI	TGTACA	2 cut(s)	642, 1318
BsrI	ACTGG	5 cut(s)	1271, 1444, 1883, 2053, 2085
BssECI	CCNNGG	7 cut(s)	327, 351, 492, 529, 888, 1951, 1957
BssMI	GATC	6 cut(s)	11, 603, 814, 1017, 1605, 2358
BssNAI	GTATAC	1 cut(s)	1098
BssNI	GRCGYC	3 cut(s)	171, 186, 1266
BssT1I	CCWWGG	3 cut(s)	351, 888, 1951
Bst1107I	GTATAC	1 cut(s)	1098
Bst2UI	CCWGG	3 cut(s)	530, 927, 1562
Bst4CI	ACNGT	7 cut(s)	418, 637, 883, 1001, 1418, 1535, 1910
Bst6I	CTCTTC	6 cut(s)	83, 100, 105, 455, 1140, 2013
BstACI	GRCGYC	3 cut(s)	171, 186, 1266
BstAUI	TGTACA	2 cut(s)	642, 1318
BstBAI	YACGTR	1 cut(s)	1347
BstC8I	GCNNGC	5 cut(s)	190, 304, 866, 1287, 2313
BstDSI	CCRYGG	2 cut(s)	327, 1951
BstENI	CCTNNNNNAGG	1 cut(s)	758
BstF5I	GGATG	3 cut(s)	739, 1203, 2248
BstFNI	CGCG	3 cut(s)	141, 192, 1582
BstH2I	RGCGCY	2 cut(s)	189, 303
BstKTI	GATC	6 cut(s)	14, 606, 817, 1020, 1608, 2361
BstMAI	GTCTC	3 cut(s)	62, 581, 2093
BstMBI	GATC	6 cut(s)	11, 603, 814, 1017, 1605, 2358
BstMWI	GCNNNNNNNGC	6 cut(s)	170, 194, 200, 289, 434, 1864
BstNI	CCWGG	3 cut(s)	530, 927, 1562
BstNSI	RCATGY	2 cut(s)	628, 788
BstSCI	CCNGG	5 cut(s)	491, 528, 925, 1560, 1956
BstSFI	CTRYAG	4 cut(s)	1089, 1464, 2125, 2226
BstSLI	GKGCMC	1 cut(s)	1378
BstUI	CGCG	3 cut(s)	141, 192, 1582
BstV1I	GCAGC	9 cut(s)	270, 976, 1075, 1163, 1450, 1780, 1930, 2179, 2212
BstV2I	GAAGAC	2 cut(s)	419, 2301
BstX2I	RGATCY	1 cut(s)	814
BstXI	CCANNNNNNTGG	1 cut(s)	2157
BstYI	RGATCY	1 cut(s)	814
BstZ17I	GTATAC	1 cut(s)	1098
Bsu36I	CCTNAGG	1 cut(s)	654
BsuI	GTATCC	2 cut(s)	1327, 1458
BsuRI	GGCC	5 cut(s)	528, 752, 1279, 1656, 1956
BtgI	CCRYGG	2 cut(s)	327, 1951
BtrI	CACGTC	1 cut(s)	2276
BtsCI	GGATG	3 cut(s)	739, 1203, 2248
BtsI	GCAGTG	2 cut(s)	1098, 1473
BtsIMutI	CAGTG	7 cut(s)	423, 993, 1098, 1437, 1473, 2046, 2055
BveI	ACCTGC	1 cut(s)	2320
Cac8I	GCNNGC	5 cut(s)	190, 304, 866, 1287, 2313
CciI	TCATGA	1 cut(s)	2134
Cfr13I	GGNCC	5 cut(s)	526, 751, 923, 1277, 2197
CseI	GACGC	2 cut(s)	179, 1255
CsiI	ACCWGGT	1 cut(s)	925
CspCI	CAANNNNNGTGG	2 cut(s)	495, 530
DinI	GGCGCC	1 cut(s)	187
DpnI	GATC	6 cut(s)	13, 605, 816, 1019, 1607, 2360
DpnII	GATC	6 cut(s)	11, 603, 814, 1017, 1605, 2358
EaeI	YGGCCR	2 cut(s)	1654, 1954
Eam1104I	CTCTTC	6 cut(s)	83, 100, 105, 455, 1140, 2013
EarI	CTCTTC	6 cut(s)	83, 100, 105, 455, 1140, 2013
EciI	GGCGGA	3 cut(s)	41, 71, 151
Eco130I	CCWWGG	3 cut(s)	351, 888, 1951
Eco47I	GGWCC	2 cut(s)	923, 2197
Eco57I	CTGAAG	4 cut(s)	500, 798, 1013, 2079
Eco72I	CACGTG	1 cut(s)	1347
Eco81I	CCTNAGG	1 cut(s)	654
EcoNI	CCTNNNNNAGG	1 cut(s)	758
EcoO109I	RGGNCCY	2 cut(s)	751, 923
EcoRII	CCWGG	3 cut(s)	528, 925, 1560
EcoT14I	CCWWGG	3 cut(s)	351, 888, 1951
EcoT22I	ATGCAT	1 cut(s)	877
EgeI	GGCGCC	1 cut(s)	187
EheI	GGCGCC	1 cut(s)	187
ErhI	CCWWGG	3 cut(s)	351, 888, 1951
Esp3I	CGTCTC	2 cut(s)	62, 581
FalI	AAGNNNNNCTT	4 cut(s)	829, 861, 2052, 2084
FaqI	GGGAC	4 cut(s)	936, 1095, 1253, 1931
FauI	CCCGC	3 cut(s)	161, 197, 424
FbaI	TGATCA	1 cut(s)	603
FblI	GTMKAC	3 cut(s)	34, 910, 1097
FokI	GGATG	3 cut(s)	746, 1190, 2255
FspBI	CTAG	6 cut(s)	248, 914, 1142, 1286, 1575, 1925
FspI	TGCGCA	3 cut(s)	307, 705, 863
GsaI	CCCAGC	3 cut(s)	220, 812, 1728
HaeII	RGCGCY	2 cut(s)	189, 303
HaeIII	GGCC	5 cut(s)	528, 752, 1279, 1656, 1956
HapII	CCGG	7 cut(s)	183, 344, 404, 492, 1957, 2266, 2306
HgaI	GACGC	2 cut(s)	179, 1255
Hin1I	GRCGYC	3 cut(s)	171, 186, 1266
HpaII	CCGG	7 cut(s)	183, 344, 404, 492, 1957, 2266, 2306
HphI	GGTGA	4 cut(s)	404, 877, 941, 2197
Hpy166II	GTNNAC	6 cut(s)	35, 645, 911, 1004, 1098, 1174
Hpy188I	TCNGA	7 cut(s)	1017, 1032, 1134, 1312, 1813, 2173, 2326
Hpy8I	GTNNAC	6 cut(s)	35, 645, 911, 1004, 1098, 1174
Hpy99I	CGWCG	1 cut(s)	173
HpyAV	CCTTC	7 cut(s)	175, 231, 704, 934, 1131, 1567, 1869
HpyCH4III	ACNGT	7 cut(s)	418, 637, 883, 1001, 1418, 1535, 1910
HpyCH4IV	ACGT	5 cut(s)	1346, 1372, 1966, 2077, 2275
HpyCH4V	TGCA	9 cut(s)	758, 830, 875, 1076, 1091, 1251, 1466, 1623, 2228
HpyF10VI	GCNNNNNNNGC	6 cut(s)	170, 194, 200, 289, 434, 1864
HpySE526I	ACGT	5 cut(s)	1346, 1372, 1966, 2077, 2275
Hsp92I	GRCGYC	3 cut(s)	171, 186, 1266
KasI	GGCGCC	1 cut(s)	185
Kpn2I	TCCGGA	2 cut(s)	343, 2305
KpnI	GGTACC	1 cut(s)	1937
Ksp22I	TGATCA	1 cut(s)	603
Kzo9I	GATC	6 cut(s)	11, 603, 814, 1017, 1605, 2358
LguI	GCTCTTC	1 cut(s)	455
LmnI	GCTCC	6 cut(s)	346, 442, 703, 718, 805, 2308
Lsp1109I	GCAGC	9 cut(s)	270, 976, 1075, 1163, 1450, 1780, 1930, 2179, 2212
LweI	GCATC	6 cut(s)	724, 849, 862, 1780, 1834, 2233
MabI	ACCWGGT	1 cut(s)	925
MaeI	CTAG	6 cut(s)	248, 914, 1142, 1286, 1575, 1925
MaeII	ACGT	5 cut(s)	1346, 1372, 1966, 2077, 2275
MaeIII	GTNAC	4 cut(s)	620, 883, 1492, 1869
MalI	GATC	6 cut(s)	13, 605, 816, 1019, 1607, 2360
MbiI	CCGCTC	1 cut(s)	445
MboI	GATC	6 cut(s)	11, 603, 814, 1017, 1605, 2358
MfeI	CAATTG	1 cut(s)	278
MflI	RGATCY	1 cut(s)	814
MhlI	GDGCHC	1 cut(s)	1378
MlsI	TGGCCA	1 cut(s)	1656
MluNI	TGGCCA	1 cut(s)	1656
Mly113I	GGCGCC	1 cut(s)	186
MlyI	GAGTC	6 cut(s)	245, 1057, 1144, 1700, 1913, 2046
Mox20I	TGGCCA	1 cut(s)	1656
Mph1103I	ATGCAT	1 cut(s)	877
MroI	TCCGGA	2 cut(s)	343, 2305
MroXI	GAANNNNTTC	1 cut(s)	2022
MscI	TGGCCA	1 cut(s)	1656
Msp20I	TGGCCA	1 cut(s)	1656
MspI	CCGG	7 cut(s)	183, 344, 404, 492, 1957, 2266, 2306
MspR9I	CCNGG	5 cut(s)	493, 530, 927, 1562, 1958
MunI	CAATTG	1 cut(s)	278
Mva1269I	GAATGC	1 cut(s)	474
MvaI	CCWGG	3 cut(s)	530, 927, 1562
MvnI	CGCG	3 cut(s)	141, 192, 1582
MwoI	GCNNNNNNNGC	6 cut(s)	170, 194, 200, 289, 434, 1864
NarI	GGCGCC	1 cut(s)	186
NciI	CCSGG	2 cut(s)	493, 1958
NcoI	CCATGG	1 cut(s)	1951
NdeII	GATC	6 cut(s)	11, 603, 814, 1017, 1605, 2358
NheI	GCTAGC	1 cut(s)	1285
NlaIV	GGNNCC	7 cut(s)	187, 402, 527, 543, 666, 924, 1935
NmeAIII	GCCGAG	1 cut(s)	2362
NmuCI	GTSAC	2 cut(s)	883, 1869
NsbI	TGCGCA	3 cut(s)	307, 705, 863
NsiI	ATGCAT	1 cut(s)	877
NspI	RCATGY	2 cut(s)	628, 788
PagI	TCATGA	1 cut(s)	2134
PciI	ACATGT	1 cut(s)	624
PciSI	GCTCTTC	1 cut(s)	455
PcsI	WCGNNNNNNNCGW	1 cut(s)	2281
PctI	GAATGC	1 cut(s)	474
PdmI	GAANNNNTTC	1 cut(s)	2022
PfeI	GAWTC	5 cut(s)	323, 347, 932, 1234, 2146
PleI	GAGTC	6 cut(s)	245, 1057, 1143, 1700, 1913, 2046
PluTI	GGCGCC	1 cut(s)	189
PmaCI	CACGTG	1 cut(s)	1347
PmlI	CACGTG	1 cut(s)	1347
PpsI	GAGTC	6 cut(s)	245, 1057, 1143, 1700, 1913, 2046
Ppu21I	YACGTR	1 cut(s)	1347
PpuMI	RGGWCCY	1 cut(s)	923
PscI	ACATGT	1 cut(s)	624
Psp1406I	AACGTT	1 cut(s)	2077
Psp5II	RGGWCCY	1 cut(s)	923
Psp6I	CCWGG	3 cut(s)	528, 925, 1560
PspCI	CACGTG	1 cut(s)	1347
PspFI	CCCAGC	3 cut(s)	216, 808, 1724
PspGI	CCWGG	3 cut(s)	528, 925, 1560
PspN4I	GGNNCC	7 cut(s)	187, 402, 527, 543, 666, 924, 1935
PspPI	GGNCC	5 cut(s)	526, 751, 923, 1277, 2197
PspPPI	RGGWCCY	1 cut(s)	923
PstI	CTGCAG	3 cut(s)	1093, 1468, 2230
PsuI	RGATCY	1 cut(s)	814
SapI	GCTCTTC	1 cut(s)	455
Sau3AI	GATC	6 cut(s)	11, 603, 814, 1017, 1605, 2358
Sau96I	GGNCC	5 cut(s)	526, 751, 923, 1277, 2197
SchI	GAGTC	6 cut(s)	245, 1057, 1144, 1700, 1913, 2046
ScrFI	CCNGG	5 cut(s)	493, 530, 927, 1562, 1958
SduI	GDGCHC	1 cut(s)	1378
SexAI	ACCWGGT	1 cut(s)	925
SfaNI	GCATC	6 cut(s)	724, 849, 862, 1780, 1834, 2233
SfcI	CTRYAG	4 cut(s)	1089, 1464, 2125, 2226
SfoI	GGCGCC	1 cut(s)	187
SinI	GGWCC	2 cut(s)	923, 2197
SmlI	CTYRAG	2 cut(s)	369, 2352
SmoI	CTYRAG	2 cut(s)	369, 2352
SspDI	GGCGCC	1 cut(s)	185
SspMI	CTAG	6 cut(s)	248, 914, 1142, 1286, 1575, 1925
StyD4I	CCNGG	5 cut(s)	491, 528, 925, 1560, 1956
StyI	CCWWGG	3 cut(s)	351, 888, 1951
TaaI	ACNGT	7 cut(s)	418, 637, 883, 1001, 1418, 1535, 1910
TaiI	ACGT	5 cut(s)	1349, 1375, 1969, 2080, 2278
TaqI	TCGA	8 cut(s)	168, 570, 595, 740, 1112, 1420, 1472, 2284
TatI	WGTACW	4 cut(s)	642, 899, 1173, 1318
TauI	GCSGC	6 cut(s)	197, 200, 203, 431, 475, 686
TfiI	GAWTC	5 cut(s)	323, 347, 932, 1234, 2146
TscAI	CASTG	7 cut(s)	423, 1000, 1098, 1444, 1473, 2053, 2062
TseFI	GTSAC	2 cut(s)	883, 1869
TseI	GCWGC	9 cut(s)	283, 989, 1088, 1151, 1463, 1793, 1943, 2192, 2225
Tsp45I	GTSAC	2 cut(s)	883, 1869
TspDTI	ATGAA	7 cut(s)	12, 723, 1378, 1785, 2031, 2159, 2207
TspGWI	ACGGA	4 cut(s)	316, 623, 1010, 2081
TspRI	CASTG	7 cut(s)	423, 1000, 1098, 1444, 1473, 2053, 2062
VpaK11BI	GGWCC	2 cut(s)	923, 2197
XagI	CCTNNNNNAGG	1 cut(s)	758
XapI	RAATTY	2 cut(s)	497, 1548
XbaI	TCTAGA	1 cut(s)	1141
XceI	RCATGY	2 cut(s)	628, 788
XmiI	GTMKAC	3 cut(s)	34, 910, 1097
XmnI	GAANNNNTTC	1 cut(s)	2022
XspI	CTAG	6 cut(s)	248, 914, 1142, 1286, 1575, 1925
Zsp2I	ATGCAT	1 cut(s)	877

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.