Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0010089

Physical Location & Seq

Genomic Coordinates Reverse (-)

13919681 .. 13926212

Gene Structure 6532 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1147600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1533 bp

ATGCACAAGCCTGATGCATTAACAGTCGCCAAGGTTTTCAGTACATTTCGTCAACTTGCTAAGGAATCTGGGAAGGATAGTCAGGAGAGGAAAAAAAACCATATCAAGGCACTTCTTGTTGCTGCTACTGACCGTGAACCCTTATATCTGATCCGTTTGCTTCAGACAAAGTTGCGAATTGGATTGGCAGAGCAGACTCTTTTAGCTGCATTGGGACAAGCTGCAGTGTATACTGAAAAAAATTCTACACCACCTCCTCATATTCAGAGTCCTCTAGAAGAGGCTGCAAAAATAGTTAAGCAAGTGTACTCTCTGCTTCCTGATTACGATAAAATCGTCCCTGCCCTTTTAAGTGGTGGCGTGTGGAATCTTCCAGAGATATGCAACTTTACTTTGGGTGTCCCAGTTGGACCTATGCTAGCAAAACCAACTAAAGGGGTATCTGAGATTGTACAGAAGTTTCAGGATACAGAATACACATGTGAATACAAGTATGATGGAGAACGTGCCCAGATACATTTCATGGAAAATGGTTCGGTTGAGATATACAGTCGAAATGCTGAAAGAAACACCGGAAAGTACCCTGATGTTGTTGTTGCAGTGTCGAGGTTAAAGAAGTCATCTGTAACATCATTTGTTTTGGATTGCGAAATTGTTGCTTACGACCGTGAAAAACAGAAAATTCTGCCCTTCCAGGTACTCAGCACTAGACCTCGTAAAAATGTGAGCGTGAGTGATATCAAGGTTGAAGTCTGCATATTTTCTTTTGATATGTTGTATCTTAATGGCCGACCACTTATTCAGGAACAACTGAAAGTTCGTAGAGAGTGTCTGTATGACTCTTTTGTGGAAGAAGCTGGATTTTTTCAGTTTGCCACAGCCTTAACATCAAATGATATAGATGAAATACAAAAGTTTCTTGATGCTGCTGTCAGTTCAAGTTCCGAGGGTTTAATTATTAAAACATTGAACAAAGATGCTACCTACGAGCCTTCAAAGCGGTCACTTAACTGGCTGAAATTGAAGAAAGATTACATGGACAGTATTGGGGACTCACTAGACTTGGTACCTATTGCTGCTTTCCATGGCCGGGGAAAACGTACAGGTGTCTATGGTGCATTCCTCCTCGCTTGTTATGATAGCAATAATGAAGAGTTTCAAAGCATTTGTAAAGTAGGCACTGGACTCACCGAAGCAATGCTTGAAGAACGCTCTGCCAGTCTCCTTACGAAAATGATACCCAAACCTAAGCCATATTATAGGTATTCTGATTCATTGAATCCAGATGTATGGTTTGATCCCTCAGAGGTTTGGGAGGTGAAAGCTGCGGACCTGACTATTAGCCCCGTTCATCGTGCTGCAGTTGGCATAGTGGATGCTAATAAGGGTATCTCTCTCCGCTTTCCACGTCTAATTCGTGTTCGGGAAGATAAACCTCCAGAGCAAGCCACATCGTCTGAACAGGTAGCCGAGTTGTATAATGCTCAAGCCATCAACCATTCAAACAATCAAAATGACAACGGAGATGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000012 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006304 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016874 GO:0016886 GO:0022616 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0034645 GO:0035510 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044728 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0070013 GO:0070988 GO:0071704 GO:0080111 GO:0090304 GO:0140097 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03030 ko03410 ko03420 ko03430 map03030 map03410 map03420 map03430

Pfam Domains

Protein Families

DNA_ligase_A_C DNA_ligase_A_M DNA_ligase_A_N

Protein Analysis

511

Amino Acids

57.18

Weight (kDa)

6.26

Isoelectric Point (pI)

43.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_ligase_A_N	PF04675	3 - 71	6e-18	DNA ligase N terminus
DNA_ligase_A_M	PF01068	138 - 342	3e-67	ATP dependent DNA ligase domain
DNA_ligase_A_C	PF04679	367 - 478	1.3e-21	ATP dependent DNA ligase C terminal region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08130
fragaria_vesca	FvH4_3g30730 FvH4_3g30731 FvH4_3g30740
malus_domestica	MD03G1127000.v1.1 MD11G1147600.v1.1
prunus_persica	Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1 Prupe.6G112300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0056931
rosa_laevigata	RLG00000035140
rosa_multiflora	Rmu_sc0002035.1_g000008
rosa_roxburghii	Rroxscaffold_1G00023260 Rroxscaffold_1G00059860
rosa_rugosa	Rorug05G0304400
rosa_samantha	Rh5AG373800 Rh5BG384800 Rh5CG408100
rosa_wichuraiana	Rw5G035180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1066
AccB1I	GGYRCC	1 cut(s)	1066
AccI	GTMKAC	1 cut(s)	230
AciI	CCGC	3 cut(s)	1000, 1328, 1399
AclWI	GGATC	2 cut(s)	145, 1292
AcoI	YGGCCR	2 cut(s)	787, 1087
AcsI	RAATTY	2 cut(s)	241, 681
AcuI	CTGAAG	1 cut(s)	146
AfaI	GTAC	7 cut(s)	43, 308, 453, 581, 699, 1068, 1102
AfiI	CCNNNNNNNGG	3 cut(s)	106, 434, 1090
AflIII	ACRYGT	1 cut(s)	479
AgsI	TTSAA	9 cut(s)	749, 939, 970, 996, 1024, 1160, 1205, 1279, 1503
AjiI	CACGTC	1 cut(s)	1409
AjnI	CCWGG	1 cut(s)	693
AluBI	AGCT	4 cut(s)	206, 221, 857, 1325
AluI	AGCT	4 cut(s)	206, 221, 857, 1325
Alw26I	GTCTC	1 cut(s)	1226
AlwI	GGATC	2 cut(s)	145, 1292
AoxI	GGCC	2 cut(s)	787, 1087
ApeKI	GCWGC	8 cut(s)	122, 206, 221, 284, 926, 1076, 1325, 1358
ApoI	RAATTY	2 cut(s)	241, 681
Asp700I	GAANNNNTTC	1 cut(s)	1155
Asp718I	GGTACC	1 cut(s)	1066
AspS9I	GGNCC	2 cut(s)	410, 1330
AsuC2I	CCSGG	1 cut(s)	1091
AsuHPI	GGTGA	2 cut(s)	1180, 1330
AsuNHI	GCTAGC	1 cut(s)	418
AvaII	GGWCC	2 cut(s)	410, 1330
BaeGI	GKGCMC	1 cut(s)	511
BanI	GGYRCC	1 cut(s)	1066
BarI	GAAGNNNNNNTAC	2 cut(s)	1016, 1048
BbvI	GCAGC	8 cut(s)	109, 193, 208, 271, 913, 1063, 1312, 1345
BccI	CCATC	2 cut(s)	491, 1499
BcgI	CGANNNNNNTGC	2 cut(s)	638, 672
BciT130I	CCWGG	1 cut(s)	695
BciVI	GTATCC	1 cut(s)	460
BcnI	CCSGG	1 cut(s)	1091
BcoDI	GTCTC	1 cut(s)	1226
BfaI	CTAG	4 cut(s)	275, 419, 708, 1058
BfmI	CTRYAG	2 cut(s)	222, 1359
BfuI	GTATCC	1 cut(s)	460
BisI	GCNGC	8 cut(s)	123, 207, 222, 285, 927, 1077, 1326, 1359
BlsI	GCNGC	8 cut(s)	124, 208, 223, 286, 928, 1078, 1327, 1360
Bme1390I	CCNGG	2 cut(s)	695, 1091
Bme18I	GGWCC	2 cut(s)	410, 1330
BmgBI	CACGTC	1 cut(s)	1409
BmgT120I	GGNCC	2 cut(s)	410, 1330
BmiI	GGNNCC	1 cut(s)	1068
BmrFI	CCNGG	2 cut(s)	695, 1091
BmrI	ACTGGG	1 cut(s)	398
BmsI	GCATC	4 cut(s)	4, 913, 967, 1366
BmtI	GCTAGC	1 cut(s)	422
BmuI	ACTGGG	1 cut(s)	398
BpmI	CTGGAG	1 cut(s)	1422
Bpu10I	CCTNAGC	2 cut(s)	60, 1248
BpuEI	CTTGAG	1 cut(s)	1470
BpuMI	CCSGG	1 cut(s)	1091
BsaJI	CCNNGG	4 cut(s)	30, 945, 1084, 1090
BsaWI	WCCGGW	1 cut(s)	572
BsaXI	ACNNNNNCTCC	2 cut(s)	238, 268
Bsc4I	CCNNNNNNNGG	3 cut(s)	106, 434, 1090
Bse1I	ACTGG	4 cut(s)	404, 1016, 1186, 1218
Bse3DI	GCAATG	1 cut(s)	1203
BseBI	CCWGG	1 cut(s)	695
BseDI	CCNNGG	4 cut(s)	30, 945, 1084, 1090
BseGI	GGATG	1 cut(s)	1381
BseLI	CCNNNNNNNGG	3 cut(s)	106, 434, 1090
BseMI	GCAATG	1 cut(s)	1203
BseMII	CTCAG	3 cut(s)	435, 715, 1317
BseNI	ACTGG	4 cut(s)	404, 1016, 1186, 1218
BseRI	GAGGAG	2 cut(s)	246, 1115
BseSI	GKGCMC	1 cut(s)	511
BseXI	GCAGC	8 cut(s)	109, 193, 208, 271, 913, 1063, 1312, 1345
Bsh1285I	CGRYCG	1 cut(s)	667
BshFI	GGCC	2 cut(s)	789, 1089
BshNI	GGYRCC	1 cut(s)	1066
BsiEI	CGRYCG	1 cut(s)	667
BsiSI	CCGG	2 cut(s)	573, 1090
BslFI	GGGAC	4 cut(s)	228, 323, 386, 1064
BslI	CCNNNNNNNGG	3 cut(s)	106, 434, 1090
BsmAI	GTCTC	1 cut(s)	1226
BsmFI	GGGAC	4 cut(s)	228, 323, 386, 1064
BsmI	GAATGC	1 cut(s)	1118
BsnI	GGCC	2 cut(s)	789, 1089
Bsp1286I	GDGCHC	1 cut(s)	511
Bsp1407I	TGTACA	1 cut(s)	451
Bsp143I	GATC	2 cut(s)	150, 1297
Bsp19I	CCATGG	1 cut(s)	1084
BspACI	CCGC	3 cut(s)	1000, 1328, 1399
BspANI	GGCC	2 cut(s)	789, 1089
BspCNI	CTCAG	3 cut(s)	436, 714, 1316
BspLI	GGNNCC	1 cut(s)	1068
BspMAI	CTGCAG	2 cut(s)	226, 1363
BspOI	GCTAGC	1 cut(s)	422
BspPI	GGATC	2 cut(s)	145, 1292
BspT107I	GGYRCC	1 cut(s)	1066
BsrDI	GCAATG	1 cut(s)	1203
BsrGI	TGTACA	1 cut(s)	451
BsrI	ACTGG	4 cut(s)	404, 1016, 1186, 1218
BssECI	CCNNGG	4 cut(s)	30, 945, 1084, 1090
BssMI	GATC	2 cut(s)	150, 1297
BssNAI	GTATAC	1 cut(s)	231
BssT1I	CCWWGG	2 cut(s)	30, 1084
Bst1107I	GTATAC	1 cut(s)	231
Bst2UI	CCWGG	1 cut(s)	695
Bst4CI	ACNGT	5 cut(s)	25, 134, 551, 668, 1043
Bst6I	CTCTTC	2 cut(s)	273, 1146
BstAUI	TGTACA	1 cut(s)	451
BstC8I	GCNNGC	2 cut(s)	420, 1446
BstDEI	CTNAG	5 cut(s)	60, 444, 701, 1248, 1303
BstDSI	CCRYGG	1 cut(s)	1084
BstF5I	GGATG	1 cut(s)	1381
BstKTI	GATC	2 cut(s)	153, 1300
BstMAI	GTCTC	1 cut(s)	1226
BstMBI	GATC	2 cut(s)	150, 1297
BstMCI	CGRYCG	1 cut(s)	667
BstMWI	GCNNNNNNNGC	1 cut(s)	997
BstNI	CCWGG	1 cut(s)	695
BstNSI	RCATGY	1 cut(s)	483
BstSCI	CCNGG	2 cut(s)	693, 1089
BstSFI	CTRYAG	2 cut(s)	222, 1359
BstSLI	GKGCMC	1 cut(s)	511
BstV1I	GCAGC	8 cut(s)	109, 193, 208, 271, 913, 1063, 1312, 1345
BstXI	CCANNNNNNTGG	1 cut(s)	1290
BstZ17I	GTATAC	1 cut(s)	231
BsuI	GTATCC	1 cut(s)	460
BsuRI	GGCC	2 cut(s)	789, 1089
BtgI	CCRYGG	1 cut(s)	1084
BtrI	CACGTC	1 cut(s)	1409
BtsCI	GGATG	1 cut(s)	1381
BtsI	GCAGTG	2 cut(s)	231, 606
BtsIMutI	CAGTG	3 cut(s)	231, 606, 1179
Cac8I	GCNNGC	2 cut(s)	420, 1446
Cfr13I	GGNCC	2 cut(s)	410, 1330
Csp6I	GTAC	7 cut(s)	42, 307, 452, 580, 698, 1067, 1101
CviAII	CATG	4 cut(s)	480, 523, 1036, 1085
CviQI	GTAC	7 cut(s)	42, 307, 452, 580, 698, 1067, 1101
DdeI	CTNAG	5 cut(s)	60, 444, 701, 1248, 1303
DpnI	GATC	2 cut(s)	152, 1299
DpnII	GATC	2 cut(s)	150, 1297
EaeI	YGGCCR	2 cut(s)	787, 1087
Eam1104I	CTCTTC	2 cut(s)	273, 1146
EarI	CTCTTC	2 cut(s)	273, 1146
Eco130I	CCWWGG	2 cut(s)	30, 1084
Eco32I	GATATC	1 cut(s)	739
Eco47I	GGWCC	2 cut(s)	410, 1330
Eco57I	CTGAAG	1 cut(s)	146
EcoRII	CCWGG	1 cut(s)	693
EcoRV	GATATC	1 cut(s)	739
EcoT14I	CCWWGG	2 cut(s)	30, 1084
EcoT22I	ATGCAT	1 cut(s)	19
ErhI	CCWWGG	2 cut(s)	30, 1084
FaeI	CATG	4 cut(s)	483, 526, 1039, 1088
FalI	AAGNNNNNCTT	2 cut(s)	1185, 1217
FaqI	GGGAC	4 cut(s)	228, 323, 386, 1064
FatI	CATG	4 cut(s)	479, 522, 1035, 1084
FblI	GTMKAC	1 cut(s)	230
Fnu4HI	GCNGC	8 cut(s)	123, 207, 222, 285, 927, 1077, 1326, 1359
FokI	GGATG	1 cut(s)	1388
Fsp4HI	GCNGC	8 cut(s)	123, 207, 222, 285, 927, 1077, 1326, 1359
FspBI	CTAG	4 cut(s)	275, 419, 708, 1058
GluI	GCNGC	8 cut(s)	123, 207, 222, 285, 927, 1077, 1326, 1359
GsuI	CTGGAG	1 cut(s)	1422
HaeIII	GGCC	2 cut(s)	789, 1089
HapII	CCGG	2 cut(s)	573, 1090
Hin1II	CATG	4 cut(s)	483, 526, 1039, 1088
HincII	GTYRAC	1 cut(s)	53
HindII	GTYRAC	1 cut(s)	53
HinfI	GANTC	9 cut(s)	65, 196, 268, 367, 839, 1052, 1185, 1271, 1279
HpaII	CCGG	2 cut(s)	573, 1090
HphI	GGTGA	2 cut(s)	1180, 1330
Hpy166II	GTNNAC	4 cut(s)	53, 137, 231, 307
Hpy188I	TCNGA	8 cut(s)	150, 165, 267, 445, 946, 1270, 1306, 1459
Hpy8I	GTNNAC	4 cut(s)	53, 137, 231, 307
HpyAV	CCTTC	3 cut(s)	67, 700, 1002
HpyCH4III	ACNGT	5 cut(s)	25, 134, 551, 668, 1043
HpyCH4IV	ACGT	3 cut(s)	505, 1099, 1408
HpyF10VI	GCNNNNNNNGC	1 cut(s)	997
HpyF3I	CTNAG	5 cut(s)	60, 444, 701, 1248, 1303
HpySE526I	ACGT	3 cut(s)	505, 1099, 1408
Hsp92II	CATG	4 cut(s)	483, 526, 1039, 1088
KpnI	GGTACC	1 cut(s)	1070
Kzo9I	GATC	2 cut(s)	150, 1297
Lsp1109I	GCAGC	8 cut(s)	109, 193, 208, 271, 913, 1063, 1312, 1345
LweI	GCATC	4 cut(s)	4, 913, 967, 1366
MaeI	CTAG	4 cut(s)	275, 419, 708, 1058
MaeII	ACGT	3 cut(s)	505, 1099, 1408
MaeIII	GTNAC	2 cut(s)	625, 1002
MalI	GATC	2 cut(s)	152, 1299
MboI	GATC	2 cut(s)	150, 1297
MboII	GAAGA	7 cut(s)	290, 362, 863, 1036, 1163, 1217, 1439
MhlI	GDGCHC	1 cut(s)	511
MluCI	AATT	7 cut(s)	177, 241, 651, 681, 954, 1019, 1413
MlyI	GAGTC	5 cut(s)	190, 277, 833, 1046, 1179
MmeI	TCCRAC	1 cut(s)	388
Mph1103I	ATGCAT	1 cut(s)	19
MroXI	GAANNNNTTC	1 cut(s)	1155
MseI	TTAA	9 cut(s)	20, 297, 350, 611, 783, 884, 953, 960, 1008
MspI	CCGG	2 cut(s)	573, 1090
MspR9I	CCNGG	2 cut(s)	695, 1091
Mva1269I	GAATGC	1 cut(s)	1118
MvaI	CCWGG	1 cut(s)	695
MwoI	GCNNNNNNNGC	1 cut(s)	997
NciI	CCSGG	1 cut(s)	1091
NcoI	CCATGG	1 cut(s)	1084
NdeII	GATC	2 cut(s)	150, 1297
NheI	GCTAGC	1 cut(s)	418
NlaIII	CATG	4 cut(s)	483, 526, 1039, 1088
NlaIV	GGNNCC	1 cut(s)	1068
NmeAIII	GCCGAG	1 cut(s)	1495
NmuCI	GTSAC	1 cut(s)	1002
NsiI	ATGCAT	1 cut(s)	19
NspI	RCATGY	1 cut(s)	483
PciI	ACATGT	1 cut(s)	479
PcsI	WCGNNNNNNNCGW	2 cut(s)	333, 1414
PctI	GAATGC	1 cut(s)	1118
PdmI	GAANNNNTTC	1 cut(s)	1155
PfeI	GAWTC	4 cut(s)	65, 367, 1271, 1279
PkrI	GCNGC	8 cut(s)	124, 208, 223, 286, 928, 1078, 1327, 1360
PleI	GAGTC	5 cut(s)	190, 276, 833, 1046, 1179
PpsI	GAGTC	5 cut(s)	190, 276, 833, 1046, 1179
PscI	ACATGT	1 cut(s)	479
Psp6I	CCWGG	1 cut(s)	693
PspGI	CCWGG	1 cut(s)	693
PspN4I	GGNNCC	1 cut(s)	1068
PspPI	GGNCC	2 cut(s)	410, 1330
PstI	CTGCAG	2 cut(s)	226, 1363
RsaI	GTAC	7 cut(s)	43, 308, 453, 581, 699, 1068, 1102
RsaNI	GTAC	7 cut(s)	42, 307, 452, 580, 698, 1067, 1101
SaqAI	TTAA	9 cut(s)	20, 297, 350, 611, 783, 884, 953, 960, 1008
SatI	GCNGC	8 cut(s)	123, 207, 222, 285, 927, 1077, 1326, 1359
Sau3AI	GATC	2 cut(s)	150, 1297
Sau96I	GGNCC	2 cut(s)	410, 1330
SchI	GAGTC	5 cut(s)	190, 277, 833, 1046, 1179
ScrFI	CCNGG	2 cut(s)	695, 1091
SduI	GDGCHC	1 cut(s)	511
SfaNI	GCATC	4 cut(s)	4, 913, 967, 1366
SfcI	CTRYAG	2 cut(s)	222, 1359
SinI	GGWCC	2 cut(s)	410, 1330
SmlI	CTYRAG	1 cut(s)	1485
SmoI	CTYRAG	1 cut(s)	1485
Sse9I	AATT	7 cut(s)	177, 241, 651, 681, 954, 1019, 1413
SsiI	CCGC	3 cut(s)	1000, 1328, 1399
SspMI	CTAG	4 cut(s)	275, 419, 708, 1058
StyD4I	CCNGG	2 cut(s)	693, 1089
StyI	CCWWGG	2 cut(s)	30, 1084
TaaI	ACNGT	5 cut(s)	25, 134, 551, 668, 1043
TaiI	ACGT	3 cut(s)	508, 1102, 1411
TaqI	TCGA	2 cut(s)	553, 605
TasI	AATT	7 cut(s)	177, 241, 651, 681, 954, 1019, 1413
TatI	WGTACW	3 cut(s)	41, 306, 451
TfiI	GAWTC	4 cut(s)	65, 367, 1271, 1279
Tru1I	TTAA	9 cut(s)	20, 297, 350, 611, 783, 884, 953, 960, 1008
Tru9I	TTAA	9 cut(s)	20, 297, 350, 611, 783, 884, 953, 960, 1008
TscAI	CASTG	3 cut(s)	231, 606, 1186
TseFI	GTSAC	1 cut(s)	1002
TseI	GCWGC	8 cut(s)	122, 206, 221, 284, 926, 1076, 1325, 1358
Tsp45I	GTSAC	1 cut(s)	1002
TspDTI	ATGAA	5 cut(s)	511, 918, 1164, 1263, 1340
TspGWI	ACGGA	1 cut(s)	143
TspRI	CASTG	3 cut(s)	231, 606, 1186
VpaK11BI	GGWCC	2 cut(s)	410, 1330
XapI	RAATTY	2 cut(s)	241, 681
XbaI	TCTAGA	1 cut(s)	274
XceI	RCATGY	1 cut(s)	483
XmiI	GTMKAC	1 cut(s)	230
XmnI	GAANNNNTTC	1 cut(s)	1155
XspI	CTAG	4 cut(s)	275, 419, 708, 1058
Zsp2I	ATGCAT	1 cut(s)	19

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.