Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003056

Physical Location & Seq

Genomic Coordinates Forward (+)

14771215 .. 14774729

Gene Structure 3515 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1140500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1038 bp

ATGGTGGGCGTCTGCCGCAGATTTCGTACCAAACCCAGAGCCTTGTTTTCAGCGAAGCTCTCACCGTCGTGTTCAAGCCCTCTCATTCTCCCCCCCGTCCGGATACCCACCCAAACCGCCCACCGTCTCCTCTCCAGCGGCGTCCACCGTACGAGAAGTGCTGAAATTATGAGTGGAAGGCCGATTGCAAAGGACATAATATCAAAAGTAGCTTCTGAAATAAGTAGAATGAAAGCTGCCATTGGAAGCGTTCCTGGGTTGGCTGTGGTTTTGGTGGGCAACAGAGAGGACTCCCAAGCTTTTGTCAATATAAAGTTAAAGGCTTGTAATGAAGTTGGAATTGAAACTTCCATCGTGCGGTTGCCCCAAGATTGTACTCAAAATCGGCTTATTGATGTAGTTTCGGGTTTTAACAAGAACCCGTCTGTGCACGGTATAATTGTTCAACTCCCTCTGCCACAGCATTTGGACGAGGAAAGGGTTATCAATTTCGTTAGTCCGGAAAAAGATGTGGATGGCTTTCATCCCCTTAATATGGGGAACCTTGCATTGCGGGGAAAGGAACCGTTATTCATTCCTTGTGCTCCTAAGGCTTGCATAGAGTTGTTGCTCCGGTATGGAGTTGAGATCGTTGGGAAGAATGCGGTGGTAATTGGGAGGAGCAAGATTGCGGGATTATCCACTTCATTGCTATTGCAGAGGCACCATGCAACCGTTTGCACTGTTCATTCGTTCACTAACAACCCAGAACAAATTACTCGTCGAGCTGATATTGTTGTCTCAGATGTTGGCATTCCAAATATAGTCGGATGCGATTGGCTGATGCCAGGGGCAGTTGTAGTCGATATGGGGACAAATTCAGTAAAGGATCCTAGCAGCAGGAGAGGTTTCCGTATCACTGGAGATGTGTGCTATGAAGAGGCAGTTAAAGTGGTGTCGGCCATAACACCCGTGCCAGGAGGGGTCGGACCTGTTGTACTCTCAATGCTCCTCTCCAACATCCTTGACTCCGCAAAGCGAGCTTTTGGATTTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00670 ko01100 map00670 map01100

Pfam Domains

Protein Families

THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

346

Amino Acids

37.14

Weight (kDa)

9.48

Isoelectric Point (pI)

50.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THF_DHG_CYH	PF00763	57 - 172	2e-38	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C	PF02882	193 - 341	2.2e-47	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003056)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020
malus_domestica	MD03G1140500.v1.1
prunus_persica	Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1
pyrus_communis	pycom03g09560
rosa_chinensis	RchiOBHm_Chr2g0084241
rosa_laevigata	RLG00000015602
rosa_multiflora	Rmu_sc0003129.1_g000019
rosa_roxburghii	Rroxscaffold_2G00156220
rosa_rugosa	Rorug01G0452000
rosa_samantha	Rh2AG000400 Rh2BG000300 Rh2CG000200 Rh2DG000400
rosa_wichuraiana	Rw2G000020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	702
AccIII	TCCGGA	2 cut(s)	99, 499
AciI	CCGC	8 cut(s)	16, 117, 138, 358, 553, 644, 671, 1011
AclWI	GGATC	2 cut(s)	863, 876
AcoI	YGGCCR	1 cut(s)	939
AcsI	RAATTY	1 cut(s)	856
AcyI	GRCGYC	2 cut(s)	9, 141
AfaI	GTAC	4 cut(s)	28, 151, 376, 978
AfiI	CCNNNNNNNGG	4 cut(s)	99, 357, 535, 956
AgsI	TTSAA	3 cut(s)	75, 344, 446
AjnI	CCWGG	3 cut(s)	253, 826, 955
AleI	CACNNNNGTG	1 cut(s)	67
AluBI	AGCT	6 cut(s)	58, 212, 236, 299, 767, 1022
AluI	AGCT	6 cut(s)	58, 212, 236, 299, 767, 1022
Alw21I	GWGCWC	2 cut(s)	432, 586
Alw26I	GTCTC	2 cut(s)	131, 784
Alw44I	GTGCAC	1 cut(s)	428
AlwI	GGATC	2 cut(s)	863, 876
Aor13HI	TCCGGA	2 cut(s)	99, 499
AoxI	GGCC	2 cut(s)	179, 939
ApaLI	GTGCAC	1 cut(s)	428
ApeKI	GCWGC	2 cut(s)	236, 876
ApoI	RAATTY	1 cut(s)	856
ArsI	GACNNNNNNTTYG	2 cut(s)	745, 777
AspS9I	GGNCC	1 cut(s)	968
AsuHPI	GGTGA	1 cut(s)	54
AvaII	GGWCC	1 cut(s)	968
AxyI	CCTNAGG	1 cut(s)	588
BaeGI	GKGCMC	1 cut(s)	432
BamHI	GGATCC	1 cut(s)	868
BanI	GGYRCC	1 cut(s)	702
Bbv12I	GWGCWC	2 cut(s)	432, 586
BbvI	GCAGC	2 cut(s)	223, 888
BccI	CCATC	2 cut(s)	359, 509
BciT130I	CCWGG	3 cut(s)	255, 828, 957
BciVI	GTATCC	1 cut(s)	96
BcoDI	GTCTC	2 cut(s)	131, 784
BfaI	CTAG	1 cut(s)	873
BfuI	GTATCC	1 cut(s)	96
BisI	GCNGC	4 cut(s)	16, 139, 237, 877
BlsI	GCNGC	4 cut(s)	17, 140, 238, 878
Bme1390I	CCNGG	3 cut(s)	255, 828, 957
Bme18I	GGWCC	1 cut(s)	968
BmgT120I	GGNCC	1 cut(s)	968
BmiI	GGNNCC	4 cut(s)	542, 564, 704, 870
BmrFI	CCNGG	3 cut(s)	255, 828, 957
BmsI	GCATC	2 cut(s)	800, 813
BpmI	CTGGAG	2 cut(s)	118, 921
BsaHI	GRCGYC	2 cut(s)	9, 141
BsaJI	CCNNGG	2 cut(s)	254, 827
BsaWI	WCCGGW	3 cut(s)	99, 499, 612
Bsc4I	CCNNNNNNNGG	4 cut(s)	99, 357, 535, 956
Bse1I	ACTGG	1 cut(s)	904
Bse21I	CCTNAGG	1 cut(s)	588
Bse3DI	GCAATG	2 cut(s)	548, 686
BseAI	TCCGGA	2 cut(s)	99, 499
BseBI	CCWGG	3 cut(s)	255, 828, 957
BseDI	CCNNGG	2 cut(s)	254, 827
BseGI	GGATG	4 cut(s)	520, 523, 815, 999
BseLI	CCNNNNNNNGG	4 cut(s)	99, 357, 535, 956
BseMI	GCAATG	2 cut(s)	548, 686
BseMII	CTCAG	1 cut(s)	795
BseNI	ACTGG	1 cut(s)	904
BseRI	GAGGAG	3 cut(s)	119, 673, 980
BseSI	GKGCMC	1 cut(s)	432
BseXI	GCAGC	2 cut(s)	223, 888
BshFI	GGCC	2 cut(s)	181, 941
BshNI	GGYRCC	1 cut(s)	702
BsiHKAI	GWGCWC	2 cut(s)	432, 586
BsiSI	CCGG	3 cut(s)	100, 500, 613
BsiWI	CGTACG	1 cut(s)	149
BslFI	GGGAC	1 cut(s)	865
BslI	CCNNNNNNNGG	4 cut(s)	99, 357, 535, 956
BsmAI	GTCTC	2 cut(s)	131, 784
BsmBI	CGTCTC	1 cut(s)	131
BsmFI	GGGAC	1 cut(s)	865
BsmI	GAATGC	2 cut(s)	646, 792
BsnI	GGCC	2 cut(s)	181, 941
Bsp1286I	GDGCHC	2 cut(s)	432, 586
Bsp13I	TCCGGA	2 cut(s)	99, 499
Bsp143I	GATC	2 cut(s)	627, 868
BspACI	CCGC	8 cut(s)	16, 117, 138, 358, 553, 644, 671, 1011
BspANI	GGCC	2 cut(s)	181, 941
BspCNI	CTCAG	1 cut(s)	794
BspEI	TCCGGA	2 cut(s)	99, 499
BspLI	GGNNCC	4 cut(s)	542, 564, 704, 870
BspPI	GGATC	2 cut(s)	863, 876
BspT107I	GGYRCC	1 cut(s)	702
BsrDI	GCAATG	2 cut(s)	548, 686
BsrI	ACTGG	1 cut(s)	904
BssECI	CCNNGG	2 cut(s)	254, 827
BssMI	GATC	2 cut(s)	627, 868
BssNI	GRCGYC	2 cut(s)	9, 141
Bst2UI	CCWGG	3 cut(s)	255, 828, 957
Bst4CI	ACNGT	7 cut(s)	66, 125, 149, 434, 567, 715, 724
Bst6I	CTCTTC	1 cut(s)	912
BstACI	GRCGYC	2 cut(s)	9, 141
BstC8I	GCNNGC	2 cut(s)	595, 1020
BstDEI	CTNAG	2 cut(s)	588, 781
BstF5I	GGATG	4 cut(s)	520, 523, 815, 999
BstKTI	GATC	2 cut(s)	630, 871
BstMAI	GTCTC	2 cut(s)	131, 784
BstMBI	GATC	2 cut(s)	627, 868
BstMWI	GCNNNNNNNGC	3 cut(s)	15, 590, 1019
BstNI	CCWGG	3 cut(s)	255, 828, 957
BstSCI	CCNGG	3 cut(s)	253, 826, 955
BstSLI	GKGCMC	1 cut(s)	432
BstV1I	GCAGC	2 cut(s)	223, 888
BstX2I	RGATCY	1 cut(s)	868
BstYI	RGATCY	1 cut(s)	868
Bsu36I	CCTNAGG	1 cut(s)	588
BsuI	GTATCC	1 cut(s)	96
BsuRI	GGCC	2 cut(s)	181, 941
BtsCI	GGATG	4 cut(s)	520, 523, 815, 999
BtsIMutI	CAGTG	2 cut(s)	720, 897
Cac8I	GCNNGC	2 cut(s)	595, 1020
Cfr13I	GGNCC	1 cut(s)	968
CseI	GACGC	1 cut(s)	130
Csp6I	GTAC	4 cut(s)	27, 150, 375, 977
CspCI	CAANNNNNGTGG	2 cut(s)	447, 482
CviAII	CATG	1 cut(s)	707
CviQI	GTAC	4 cut(s)	27, 150, 375, 977
DdeI	CTNAG	2 cut(s)	588, 781
DpnI	GATC	2 cut(s)	629, 870
DpnII	GATC	2 cut(s)	627, 868
EaeI	YGGCCR	1 cut(s)	939
Eam1104I	CTCTTC	1 cut(s)	912
EarI	CTCTTC	1 cut(s)	912
Eco47I	GGWCC	1 cut(s)	968
Eco81I	CCTNAGG	1 cut(s)	588
EcoRII	CCWGG	3 cut(s)	253, 826, 955
Esp3I	CGTCTC	1 cut(s)	131
FaeI	CATG	1 cut(s)	710
FaqI	GGGAC	1 cut(s)	865
FatI	CATG	1 cut(s)	706
FauI	CCCGC	2 cut(s)	546, 664
Fnu4HI	GCNGC	4 cut(s)	16, 139, 237, 877
FokI	GGATG	4 cut(s)	510, 527, 822, 986
Fsp4HI	GCNGC	4 cut(s)	16, 139, 237, 877
FspBI	CTAG	1 cut(s)	873
GluI	GCNGC	4 cut(s)	16, 139, 237, 877
GsuI	CTGGAG	2 cut(s)	118, 921
HaeIII	GGCC	2 cut(s)	181, 941
HapII	CCGG	3 cut(s)	100, 500, 613
HgaI	GACGC	1 cut(s)	130
Hin1I	GRCGYC	2 cut(s)	9, 141
Hin1II	CATG	1 cut(s)	710
HindIII	AAGCTT	1 cut(s)	297
HinfI	GANTC	2 cut(s)	290, 1007
HpaII	CCGG	3 cut(s)	100, 500, 613
HphI	GGTGA	1 cut(s)	54
Hpy166II	GTNNAC	3 cut(s)	145, 430, 735
Hpy188I	TCNGA	4 cut(s)	217, 784, 809, 968
Hpy188III	TCNNGA	2 cut(s)	100, 500
Hpy8I	GTNNAC	3 cut(s)	145, 430, 735
Hpy99I	CGWCG	2 cut(s)	70, 765
HpyAV	CCTTC	1 cut(s)	171
HpyCH4III	ACNGT	7 cut(s)	66, 125, 149, 434, 567, 715, 724
HpyCH4V	TGCA	7 cut(s)	188, 430, 548, 597, 697, 710, 720
HpyF10VI	GCNNNNNNNGC	3 cut(s)	15, 590, 1019
HpyF3I	CTNAG	2 cut(s)	588, 781
Hsp92I	GRCGYC	2 cut(s)	9, 141
Hsp92II	CATG	1 cut(s)	710
Kpn2I	TCCGGA	2 cut(s)	99, 499
Kzo9I	GATC	2 cut(s)	627, 868
LmnI	GCTCC	4 cut(s)	589, 615, 660, 993
Lsp1109I	GCAGC	2 cut(s)	223, 888
LweI	GCATC	2 cut(s)	800, 813
MaeI	CTAG	1 cut(s)	873
MalI	GATC	2 cut(s)	629, 870
MboI	GATC	2 cut(s)	627, 868
MboII	GAAGA	2 cut(s)	649, 929
MflI	RGATCY	1 cut(s)	868
MhlI	GDGCHC	2 cut(s)	432, 586
MluCI	AATT	7 cut(s)	165, 339, 438, 487, 651, 753, 856
MlyI	GAGTC	2 cut(s)	284, 1001
MmeI	TCCRAC	4 cut(s)	316, 787, 946, 1020
MroI	TCCGGA	2 cut(s)	99, 499
MseI	TTAA	4 cut(s)	317, 411, 531, 927
MslI	CAYNNNNRTG	1 cut(s)	67
MspA1I	CMGCKG	1 cut(s)	138
MspI	CCGG	3 cut(s)	100, 500, 613
MspR9I	CCNGG	3 cut(s)	255, 828, 957
Mva1269I	GAATGC	2 cut(s)	646, 792
MvaI	CCWGG	3 cut(s)	255, 828, 957
MwoI	GCNNNNNNNGC	3 cut(s)	15, 590, 1019
NdeII	GATC	2 cut(s)	627, 868
NlaIII	CATG	1 cut(s)	710
NlaIV	GGNNCC	4 cut(s)	542, 564, 704, 870
OliI	CACNNNNGTG	1 cut(s)	67
PctI	GAATGC	2 cut(s)	646, 792
Pfl23II	CGTACG	1 cut(s)	149
PkrI	GCNGC	4 cut(s)	17, 140, 238, 878
PleI	GAGTC	2 cut(s)	284, 1001
PpsI	GAGTC	2 cut(s)	284, 1001
Psp6I	CCWGG	3 cut(s)	253, 826, 955
PspGI	CCWGG	3 cut(s)	253, 826, 955
PspLI	CGTACG	1 cut(s)	149
PspN4I	GGNNCC	4 cut(s)	542, 564, 704, 870
PspPI	GGNCC	1 cut(s)	968
PsuI	RGATCY	1 cut(s)	868
RsaI	GTAC	4 cut(s)	28, 151, 376, 978
RsaNI	GTAC	4 cut(s)	27, 150, 375, 977
RseI	CAYNNNNRTG	1 cut(s)	67
SaqAI	TTAA	4 cut(s)	317, 411, 531, 927
SatI	GCNGC	4 cut(s)	16, 139, 237, 877
Sau3AI	GATC	2 cut(s)	627, 868
Sau96I	GGNCC	1 cut(s)	968
SchI	GAGTC	2 cut(s)	284, 1001
ScrFI	CCNGG	3 cut(s)	255, 828, 957
SduI	GDGCHC	2 cut(s)	432, 586
SetI	ASST	9 cut(s)	60, 214, 238, 301, 546, 769, 889, 973, 1024
SfaNI	GCATC	2 cut(s)	800, 813
SinI	GGWCC	1 cut(s)	968
SmiMI	CAYNNNNRTG	1 cut(s)	67
Sse9I	AATT	7 cut(s)	165, 339, 438, 487, 651, 753, 856
SsiI	CCGC	8 cut(s)	16, 117, 138, 358, 553, 644, 671, 1011
SspMI	CTAG	1 cut(s)	873
StyD4I	CCNGG	3 cut(s)	253, 826, 955
TaaI	ACNGT	7 cut(s)	66, 125, 149, 434, 567, 715, 724
TaqI	TCGA	2 cut(s)	763, 843
TasI	AATT	7 cut(s)	165, 339, 438, 487, 651, 753, 856
TatI	WGTACW	2 cut(s)	374, 976
TauI	GCSGC	2 cut(s)	18, 141
Tru1I	TTAA	4 cut(s)	317, 411, 531, 927
Tru9I	TTAA	4 cut(s)	317, 411, 531, 927
TscAI	CASTG	2 cut(s)	727, 904
TseI	GCWGC	2 cut(s)	236, 876
TspDTI	ATGAA	7 cut(s)	245, 345, 512, 562, 675, 716, 930
TspGWI	ACGGA	1 cut(s)	881
TspRI	CASTG	2 cut(s)	727, 904
VneI	GTGCAC	1 cut(s)	428
VpaK11BI	GGWCC	1 cut(s)	968
XapI	RAATTY	1 cut(s)	856
XspI	CTAG	1 cut(s)	873

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.