Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0003056

Physical Location & Seq

Genomic Coordinates Forward (+)

61503 .. 63838

Gene Structure 2336 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG000400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1005 bp

ATGGGGGTCTGGCGCAGATTGGGTACAAAAGCTGAAGTCTTGTATTCGACGACGTTCTCATCGTCATGGTCGACCCCTCTCGGTCTCAGTCGCCCGCTGTCTTCTGCAGCCAGTGGTAATGGGCAGGCTGAAATAATGAGCGGAAAGCCAATCGCAAAAGACATAAGGTCCAGAGTAGCTGCTGAAATATGCAGAATGAAGGCTGCCACAGGAAGACTGCCTGGATTGGCTGTTGTTTTGGTGGGTAACAGAAAGGATTCACACACTTATGTCCATCTCAAGTTAAAAGCTTGCAATCAAGTCGGAATAGTGACTTCTATTGTTCAACTGCCCCAAGACTGTACTGAAGCTCGACTTATTGATATTGTTTCATCTTTTAACCGGAACCCGTCGGTGCACGGTATAATTGTGCAGCTCCCTCTTCCACAGCATTTAGACGAGGAAAAGATTATAAATTTTGTTAGTCCAGAAAAAGATGTGGATGGCTTTCATCCCCTCAACATGGGGAATCTTGCCATGCGGGGAAGGGAACCCTTATTTATCCCTTGTGCCCCTAAGGCTTGCATTGAATTGTTGCTCCGATATGGGGTGGAGATTATTGGGAAGAATGCAGTGGTTATAGGAAGAAGCAAGATTGTTGGGTTATCCACTTCTTTGCTATTGCAGAGGCACCACGCGACTGTTAGCACCTTGCATTCATTAACCAAGAACCCAGAAGAATTGACTCGGCGCGCTGATGTTGTCATCTCAGATGTTGGGACTCCAAACTTTGTTCAATGGGACTGGCTGAAGCCAGGGGCAGCTGTAGTTGATATGGGGACGAATCCAGTTAAGGATCCCAGTAGCCGTCATGGTTTTCGTATTACAGGAGATGTTTGCTACTCGGAGGCAATCAAGGTGGTGTCAGCCATAACTCCTGTTCCAGGAGGAGTAGGACCTGTTGTAATCTCAATGCTCCTCTCTAACACCCTTGACTCTGCTAAAAGAGCTATCGGATTAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00670 ko01100 map00670 map01100

Pfam Domains

Protein Families

THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

334

Amino Acids

35.96

Weight (kDa)

9.44

Isoelectric Point (pI)

34.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THF_DHG_CYH	PF00763	46 - 161	1.3e-39	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C	PF02882	182 - 329	3.3e-49	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003056)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020 FvH4_1g00020
malus_domestica	MD03G1140500.v1.1
prunus_persica	Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1 Prupe.6G126200_v2.0.a1
pyrus_communis	pycom03g09560
rosa_chinensis	RchiOBHm_Chr2g0084241
rosa_laevigata	RLG00000015602
rosa_multiflora	Rmu_sc0003129.1_g000019
rosa_roxburghii	Rroxscaffold_2G00156220
rosa_rugosa	Rorug01G0452000
rosa_samantha	Rh2AG000400 Rh2BG000300 Rh2CG000200 Rh2DG000400
rosa_wichuraiana	Rw2G000020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	452
AccB1I	GGYRCC	1 cut(s)	669
AccBSI	CCGCTC	1 cut(s)	141
AccI	GTMKAC	1 cut(s)	71
AccII	CGCG	2 cut(s)	677, 732
AciI	CCGC	3 cut(s)	95, 141, 520
AclWI	GGATC	2 cut(s)	830, 843
AcsI	RAATTY	1 cut(s)	454
AcuI	CTGAAG	3 cut(s)	54, 366, 809
AfaI	GTAC	2 cut(s)	25, 343
AfiI	CCNNNNNNNGG	3 cut(s)	502, 586, 923
AgsI	TTSAA	3 cut(s)	326, 569, 776
AhdI	GACNNNNNGTC	1 cut(s)	166
AjnI	CCWGG	3 cut(s)	220, 793, 922
AluBI	AGCT	7 cut(s)	32, 179, 290, 350, 415, 803, 989
AluI	AGCT	7 cut(s)	32, 179, 290, 350, 415, 803, 989
Alw21I	GWGCWC	1 cut(s)	399
Alw26I	GTCTC	1 cut(s)	89
Alw44I	GTGCAC	1 cut(s)	395
AlwI	GGATC	2 cut(s)	830, 843
ApaLI	GTGCAC	1 cut(s)	395
ApeKI	GCWGC	5 cut(s)	107, 179, 203, 412, 800
ApoI	RAATTY	1 cut(s)	454
Asp700I	GAANNNNTTC	1 cut(s)	256
AspLEI	GCGC	3 cut(s)	15, 732, 734
AspS9I	GGNCC	2 cut(s)	168, 935
AvaII	GGWCC	2 cut(s)	168, 935
AxyI	CCTNAGG	1 cut(s)	555
BaeGI	GKGCMC	2 cut(s)	399, 553
BamHI	GGATCC	1 cut(s)	835
BanI	GGYRCC	1 cut(s)	669
BbsI	GAAGAC	2 cut(s)	93, 220
Bbv12I	GWGCWC	1 cut(s)	399
BbvI	GCAGC	5 cut(s)	119, 166, 190, 424, 812
BccI	CCATC	2 cut(s)	282, 476
BceAI	ACGGC	1 cut(s)	831
BcgI	CGANNNNNNTGC	2 cut(s)	283, 317
BciT130I	CCWGG	3 cut(s)	222, 795, 924
BcoDI	GTCTC	1 cut(s)	89
BfmI	CTRYAG	2 cut(s)	105, 804
BglI	GCCNNNNNGGC	1 cut(s)	557
BisI	GCNGC	5 cut(s)	108, 180, 204, 413, 801
BlsI	GCNGC	5 cut(s)	109, 181, 205, 414, 802
Bme1390I	CCNGG	3 cut(s)	222, 795, 924
Bme18I	GGWCC	2 cut(s)	168, 935
BmeRI	GACNNNNNGTC	1 cut(s)	166
BmgT120I	GGNCC	2 cut(s)	168, 935
BmiI	GGNNCC	4 cut(s)	386, 531, 671, 837
BmrFI	CCNGG	3 cut(s)	222, 795, 924
BmrI	ACTGGG	1 cut(s)	834
BmuI	ACTGGG	1 cut(s)	834
BpiI	GAAGAC	2 cut(s)	93, 220
BpuEI	CTTGAG	1 cut(s)	263
BsaI	GGTCTC	1 cut(s)	89
BsaJI	CCNNGG	1 cut(s)	794
BsaWI	WCCGGW	1 cut(s)	381
Bsc4I	CCNNNNNNNGG	3 cut(s)	502, 586, 923
Bse1I	ACTGG	4 cut(s)	111, 788, 827, 840
Bse21I	CCTNAGG	1 cut(s)	555
BseBI	CCWGG	3 cut(s)	222, 795, 924
BseDI	CCNNGG	1 cut(s)	794
BseGI	GGATG	2 cut(s)	487, 490
BseLI	CCNNNNNNNGG	3 cut(s)	502, 586, 923
BseMII	CTCAG	2 cut(s)	100, 762
BseNI	ACTGG	4 cut(s)	111, 788, 827, 840
BsePI	GCGCGC	1 cut(s)	730
BseRI	GAGGAG	2 cut(s)	942, 947
BseSI	GKGCMC	2 cut(s)	399, 553
BseXI	GCAGC	5 cut(s)	119, 166, 190, 424, 812
BsgI	GTGCAG	1 cut(s)	431
Bsh1236I	CGCG	2 cut(s)	677, 732
BshNI	GGYRCC	1 cut(s)	669
BsiHKAI	GWGCWC	1 cut(s)	399
BsiSI	CCGG	1 cut(s)	382
BslFI	GGGAC	3 cut(s)	772, 794, 832
BslI	CCNNNNNNNGG	3 cut(s)	502, 586, 923
BsmAI	GTCTC	1 cut(s)	89
BsmFI	GGGAC	3 cut(s)	772, 794, 832
BsmI	GAATGC	2 cut(s)	613, 694
Bso31I	GGTCTC	1 cut(s)	89
Bsp1286I	GDGCHC	2 cut(s)	399, 553
Bsp143I	GATC	1 cut(s)	835
BspACI	CCGC	3 cut(s)	95, 141, 520
BspCNI	CTCAG	2 cut(s)	99, 761
BspFNI	CGCG	2 cut(s)	677, 732
BspLI	GGNNCC	4 cut(s)	386, 531, 671, 837
BspMAI	CTGCAG	1 cut(s)	109
BspPI	GGATC	2 cut(s)	830, 843
BspT107I	GGYRCC	1 cut(s)	669
BspTNI	GGTCTC	1 cut(s)	89
BsrBI	CCGCTC	1 cut(s)	141
BsrI	ACTGG	4 cut(s)	111, 788, 827, 840
BssECI	CCNNGG	1 cut(s)	794
BssHII	GCGCGC	1 cut(s)	730
BssMI	GATC	1 cut(s)	835
Bst2UI	CCWGG	3 cut(s)	222, 795, 924
Bst4CI	ACNGT	3 cut(s)	341, 401, 682
Bst6I	CTCTTC	1 cut(s)	426
BstC8I	GCNNGC	5 cut(s)	95, 126, 292, 562, 732
BstDEI	CTNAG	3 cut(s)	86, 555, 748
BstENI	CCTNNNNNAGG	1 cut(s)	921
BstF5I	GGATG	2 cut(s)	487, 490
BstFNI	CGCG	2 cut(s)	677, 732
BstHHI	GCGC	3 cut(s)	15, 732, 734
BstKTI	GATC	1 cut(s)	838
BstMAI	GTCTC	1 cut(s)	89
BstMBI	GATC	1 cut(s)	835
BstMWI	GCNNNNNNNGC	2 cut(s)	557, 986
BstNI	CCWGG	3 cut(s)	222, 795, 924
BstSCI	CCNGG	3 cut(s)	220, 793, 922
BstSFI	CTRYAG	2 cut(s)	105, 804
BstSLI	GKGCMC	2 cut(s)	399, 553
BstUI	CGCG	2 cut(s)	677, 732
BstV1I	GCAGC	5 cut(s)	119, 166, 190, 424, 812
BstV2I	GAAGAC	2 cut(s)	93, 220
BstX2I	RGATCY	1 cut(s)	835
BstYI	RGATCY	1 cut(s)	835
Bsu36I	CCTNAGG	1 cut(s)	555
BtsCI	GGATG	2 cut(s)	487, 490
BtsI	GCAGTG	1 cut(s)	618
BtsIMutI	CAGTG	2 cut(s)	118, 618
Cac8I	GCNNGC	5 cut(s)	95, 126, 292, 562, 732
CfoI	GCGC	3 cut(s)	15, 732, 734
Cfr13I	GGNCC	2 cut(s)	168, 935
Csp6I	GTAC	2 cut(s)	24, 342
CspCI	CAANNNNNGTGG	2 cut(s)	879, 914
CviAII	CATG	4 cut(s)	66, 502, 517, 851
CviQI	GTAC	2 cut(s)	24, 342
DdeI	CTNAG	3 cut(s)	86, 555, 748
DpnI	GATC	1 cut(s)	837
DpnII	GATC	1 cut(s)	835
DriI	GACNNNNNGTC	1 cut(s)	166
Eam1104I	CTCTTC	1 cut(s)	426
Eam1105I	GACNNNNNGTC	1 cut(s)	166
EarI	CTCTTC	1 cut(s)	426
Eco31I	GGTCTC	1 cut(s)	89
Eco47I	GGWCC	2 cut(s)	168, 935
Eco57I	CTGAAG	3 cut(s)	54, 366, 809
Eco81I	CCTNAGG	1 cut(s)	555
EcoNI	CCTNNNNNAGG	1 cut(s)	921
EcoO109I	RGGNCCY	1 cut(s)	935
EcoRII	CCWGG	3 cut(s)	220, 793, 922
FaeI	CATG	4 cut(s)	69, 505, 520, 854
FalI	AAGNNNNNCTT	2 cut(s)	339, 371
FaqI	GGGAC	3 cut(s)	772, 794, 832
FatI	CATG	4 cut(s)	65, 501, 516, 850
FauI	CCCGC	2 cut(s)	102, 513
FblI	GTMKAC	1 cut(s)	71
Fnu4HI	GCNGC	5 cut(s)	108, 180, 204, 413, 801
FokI	GGATG	2 cut(s)	477, 494
Fsp4HI	GCNGC	5 cut(s)	108, 180, 204, 413, 801
GlaI	GCGC	3 cut(s)	14, 731, 733
GluI	GCNGC	5 cut(s)	108, 180, 204, 413, 801
HapII	CCGG	1 cut(s)	382
HhaI	GCGC	3 cut(s)	15, 732, 734
Hin1II	CATG	4 cut(s)	69, 505, 520, 854
Hin6I	GCGC	3 cut(s)	13, 730, 732
HinP1I	GCGC	3 cut(s)	13, 730, 732
HincII	GTYRAC	1 cut(s)	72
HindII	GTYRAC	1 cut(s)	72
HindIII	AAGCTT	1 cut(s)	288
HinfI	GANTC	6 cut(s)	257, 508, 724, 760, 823, 974
HpaII	CCGG	1 cut(s)	382
Hpy166II	GTNNAC	2 cut(s)	72, 397
Hpy188I	TCNGA	5 cut(s)	305, 581, 751, 886, 995
Hpy188III	TCNNGA	2 cut(s)	171, 467
Hpy8I	GTNNAC	2 cut(s)	72, 397
Hpy99I	CGWCG	3 cut(s)	52, 55, 394
HpyAV	CCTTC	2 cut(s)	193, 519
HpyCH4III	ACNGT	3 cut(s)	341, 401, 682
HpyCH4IV	ACGT	1 cut(s)	53
HpyCH4V	TGCA	9 cut(s)	107, 192, 294, 397, 412, 564, 611, 664, 694
HpyF10VI	GCNNNNNNNGC	2 cut(s)	557, 986
HpyF3I	CTNAG	3 cut(s)	86, 555, 748
HpySE526I	ACGT	1 cut(s)	53
Hsp92II	CATG	4 cut(s)	69, 505, 520, 854
HspAI	GCGC	3 cut(s)	13, 730, 732
Kzo9I	GATC	1 cut(s)	835
LmnI	GCTCC	3 cut(s)	420, 582, 960
Lsp1109I	GCAGC	5 cut(s)	119, 166, 190, 424, 812
MaeII	ACGT	1 cut(s)	53
MaeIII	GTNAC	2 cut(s)	245, 310
MalI	GATC	1 cut(s)	837
MbiI	CCGCTC	1 cut(s)	141
MboI	GATC	1 cut(s)	835
MboII	GAAGA	6 cut(s)	93, 225, 413, 616, 636, 728
MflI	RGATCY	1 cut(s)	835
MhlI	GDGCHC	2 cut(s)	399, 553
MluCI	AATT	4 cut(s)	405, 454, 569, 719
MlyI	GAGTC	3 cut(s)	718, 754, 968
MmeI	TCCRAC	1 cut(s)	283
MnlI	CCTC	8 cut(s)	87, 429, 433, 506, 660, 880, 920, 968
MroXI	GAANNNNTTC	1 cut(s)	256
MseI	TTAA	5 cut(s)	284, 378, 701, 831, 998
MslI	CAYNNNNRTG	2 cut(s)	64, 267
MspA1I	CMGCKG	2 cut(s)	97, 803
MspI	CCGG	1 cut(s)	382
MspR9I	CCNGG	3 cut(s)	222, 795, 924
Mva1269I	GAATGC	2 cut(s)	613, 694
MvaI	CCWGG	3 cut(s)	222, 795, 924
MvnI	CGCG	2 cut(s)	677, 732
MwoI	GCNNNNNNNGC	2 cut(s)	557, 986
NdeII	GATC	1 cut(s)	835
NlaIII	CATG	4 cut(s)	69, 505, 520, 854
NlaIV	GGNNCC	4 cut(s)	386, 531, 671, 837
NmeAIII	GCCGAG	1 cut(s)	706
NmuCI	GTSAC	1 cut(s)	310
PauI	GCGCGC	1 cut(s)	730
PcsI	WCGNNNNNNNCGW	2 cut(s)	59, 68
PctI	GAATGC	2 cut(s)	613, 694
PdmI	GAANNNNTTC	1 cut(s)	256
PfeI	GAWTC	3 cut(s)	257, 508, 823
PfoI	TCCNGGA	1 cut(s)	922
PkrI	GCNGC	5 cut(s)	109, 181, 205, 414, 802
PleI	GAGTC	3 cut(s)	718, 754, 968
PpsI	GAGTC	3 cut(s)	718, 754, 968
PpuMI	RGGWCCY	1 cut(s)	935
PsiI	TTATAA	1 cut(s)	452
Psp5II	RGGWCCY	1 cut(s)	935
Psp6I	CCWGG	3 cut(s)	220, 793, 922
PspGI	CCWGG	3 cut(s)	220, 793, 922
PspN4I	GGNNCC	4 cut(s)	386, 531, 671, 837
PspPI	GGNCC	2 cut(s)	168, 935
PspPPI	RGGWCCY	1 cut(s)	935
PstI	CTGCAG	1 cut(s)	109
PsuI	RGATCY	1 cut(s)	835
PteI	GCGCGC	1 cut(s)	730
PvuII	CAGCTG	1 cut(s)	803
RsaI	GTAC	2 cut(s)	25, 343
RsaNI	GTAC	2 cut(s)	24, 342
RseI	CAYNNNNRTG	2 cut(s)	64, 267
SalI	GTCGAC	1 cut(s)	70
SaqAI	TTAA	5 cut(s)	284, 378, 701, 831, 998
SatI	GCNGC	5 cut(s)	108, 180, 204, 413, 801
Sau3AI	GATC	1 cut(s)	835
Sau96I	GGNCC	2 cut(s)	168, 935
SchI	GAGTC	3 cut(s)	718, 754, 968
ScrFI	CCNGG	3 cut(s)	222, 795, 924
SduI	GDGCHC	2 cut(s)	399, 553
SfcI	CTRYAG	2 cut(s)	105, 804
SinI	GGWCC	2 cut(s)	168, 935
SmiMI	CAYNNNNRTG	2 cut(s)	64, 267
SmlI	CTYRAG	1 cut(s)	278
SmoI	CTYRAG	1 cut(s)	278
Sse9I	AATT	4 cut(s)	405, 454, 569, 719
SsiI	CCGC	3 cut(s)	95, 141, 520
StyD4I	CCNGG	3 cut(s)	220, 793, 922
TaaI	ACNGT	3 cut(s)	341, 401, 682
TaiI	ACGT	1 cut(s)	56
TaqI	TCGA	3 cut(s)	47, 71, 352
TaqII	GACCGA	1 cut(s)	71
TasI	AATT	4 cut(s)	405, 454, 569, 719
TatI	WGTACW	1 cut(s)	341
TfiI	GAWTC	3 cut(s)	257, 508, 823
Tru1I	TTAA	5 cut(s)	284, 378, 701, 831, 998
Tru9I	TTAA	5 cut(s)	284, 378, 701, 831, 998
TscAI	CASTG	2 cut(s)	118, 618
TseFI	GTSAC	1 cut(s)	310
TseI	GCWGC	5 cut(s)	107, 179, 203, 412, 800
Tsp45I	GTSAC	1 cut(s)	310
TspDTI	ATGAA	4 cut(s)	212, 360, 479, 687
TspRI	CASTG	2 cut(s)	118, 618
VneI	GTGCAC	1 cut(s)	395
VpaK11BI	GGWCC	2 cut(s)	168, 935
XagI	CCTNNNNNAGG	1 cut(s)	921
XapI	RAATTY	1 cut(s)	454
XmiI	GTMKAC	1 cut(s)	71
XmnI	GAANNNNTTC	1 cut(s)	256

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.