RLG00000015602

Bifunctional protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003056
Physical Location & Seq
Forward (+)
86766 .. 89106
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015602

Sequence Viewer

Length: 1002 bp
ATGGGGGTCTGGCGCAGATTGGGTACAAAAGCTGAAGTCTTGTATTCGACGACGTTCTCATCGTCATGGTCGACCCCTCTCGGTCTCAGTCGCTCGCTGTCTTCTGCCAGTGGTAATGGGCAGGCTGAAATAATGAGCGGAAAGCCAATCGCAAAAGATATAAGGTCCAGAGTAGCTGCTGAAATATGCAGAATGAAGGCTGCCACAGGAAGACTGCCTGGATTGGCTGTTGTTTTGGTGGGTAACAGAAAGGATTCACACACTTATGTCCATCTCAAGTTAAAAGCTTGCAATCAAGTCGGAATAGAGACTTCTATTGTTCAACTGCCCCAAGACTGTACTGAAGCTCGACTTATTGATGTTGTTTCATCTTTTAACCGGAACCCGTCGGTGCACGGTATAATTGTGCAGCTCCCTCTTCCACGGCATTTAGACGAGGAAAAGATTATAAATTTTGTTAGTCCAGAAAAAGATGTGGATGGCTTTCATCCCCTCAACATGGGGAATCTTGCCATGCGGGGAAGGGAACCCTTATTTATCCCTTGTGCCCCTAAGGCTTGCATTGAATTGTTGCTCCGATATGGGGTGGAGATTATTGGGAAGAATGCAGTGGTTATAGGAAGAAGCAAGATTGTTGGGTTATCCACTTCTTTGCTATTGCAGAGGCACCACGCGACTGTTAGCACCTTGCATTCATTCACCAAGAACCCAGAAGAATTGACTCGTCGGGCTGATGTTGTCATCTCAGATGTTGGGACTCCAAACTTAGTTCAATGGGACTGGCTGAAGCCAGGGGCAGTTGTAGTTGATATGGGGACGAATCCAGTTAAGGATCCCAGTAGCCGTCATGGTTTTCGTATTACAGGAGATGTTTGCTACTCAGAGGCAATCAAGGTGGTGTCAGCCATAACTCCTGTTCCAGGAGGAGTAGGACCTGTTGTAATCTCAATGCTTCTCTCTAACACCCTTGACTCTGCCAAAAGAGCTTTCGGATTAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

334

Amino Acids

35.98

Weight (kDa)

9.44

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THF_DHG_CYH PF00763 45 - 160 3.7e-40 Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C PF02882 181 - 329 4.4e-50 Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 449
AccB1I GGYRCC 1 cut(s) 666
AccBSI CCGCTC 1 cut(s) 138
AccI GTMKAC 1 cut(s) 71
AccII CGCG 1 cut(s) 674
AciI CCGC 2 cut(s) 138, 517
AclWI GGATC 2 cut(s) 827, 840
AcsI RAATTY 1 cut(s) 451
AcuI CTGAAG 3 cut(s) 54, 363, 806
AfaI GTAC 2 cut(s) 25, 340
AfiI CCNNNNNNNGG 3 cut(s) 499, 583, 920
AgsI TTSAA 3 cut(s) 323, 566, 773
AjnI CCWGG 3 cut(s) 217, 790, 919
AjuI GAANNNNNNNTTGG 1 cut(s) 971
AluBI AGCT 6 cut(s) 32, 176, 287, 347, 412, 986
AluI AGCT 6 cut(s) 32, 176, 287, 347, 412, 986
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 2 cut(s) 89, 302
Alw44I GTGCAC 1 cut(s) 392
AlwI GGATC 2 cut(s) 827, 840
ApaLI GTGCAC 1 cut(s) 392
ApeKI GCWGC 3 cut(s) 176, 200, 409
ApoI RAATTY 1 cut(s) 451
Asp700I GAANNNNTTC 1 cut(s) 253
AspLEI GCGC 1 cut(s) 15
AspS9I GGNCC 2 cut(s) 165, 932
AsuHPI GGTGA 1 cut(s) 691
AvaII GGWCC 2 cut(s) 165, 932
AxyI CCTNAGG 1 cut(s) 552
BaeGI GKGCMC 2 cut(s) 396, 550
BamHI GGATCC 1 cut(s) 832
BanI GGYRCC 1 cut(s) 666
BbsI GAAGAC 2 cut(s) 93, 217
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 3 cut(s) 163, 187, 421
BccI CCATC 2 cut(s) 279, 473
BceAI ACGGC 2 cut(s) 440, 828
BcgI CGANNNNNNTGC 2 cut(s) 280, 314
BciT130I CCWGG 3 cut(s) 219, 792, 921
BcoDI GTCTC 2 cut(s) 89, 302
BglI GCCNNNNNGGC 1 cut(s) 554
BisI GCNGC 3 cut(s) 177, 201, 410
BlsI GCNGC 3 cut(s) 178, 202, 411
Bme1390I CCNGG 3 cut(s) 219, 792, 921
Bme18I GGWCC 2 cut(s) 165, 932
BmgT120I GGNCC 2 cut(s) 165, 932
BmiI GGNNCC 4 cut(s) 383, 528, 668, 834
BmrFI CCNGG 3 cut(s) 219, 792, 921
BmrI ACTGGG 1 cut(s) 831
BmuI ACTGGG 1 cut(s) 831
BpiI GAAGAC 2 cut(s) 93, 217
BpuEI CTTGAG 1 cut(s) 260
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 422, 791
BsaWI WCCGGW 1 cut(s) 378
Bsc4I CCNNNNNNNGG 3 cut(s) 499, 583, 920
Bse1I ACTGG 4 cut(s) 108, 785, 824, 837
Bse21I CCTNAGG 1 cut(s) 552
BseBI CCWGG 3 cut(s) 219, 792, 921
BseDI CCNNGG 2 cut(s) 422, 791
BseGI GGATG 2 cut(s) 484, 487
BseLI CCNNNNNNNGG 3 cut(s) 499, 583, 920
BseMII CTCAG 3 cut(s) 100, 759, 894
BseNI ACTGG 4 cut(s) 108, 785, 824, 837
BseRI GAGGAG 1 cut(s) 939
BseSI GKGCMC 2 cut(s) 396, 550
BseXI GCAGC 3 cut(s) 163, 187, 421
BsgI GTGCAG 1 cut(s) 428
Bsh1236I CGCG 1 cut(s) 674
BshNI GGYRCC 1 cut(s) 666
BsiHKAI GWGCWC 1 cut(s) 396
BsiSI CCGG 1 cut(s) 379
BslFI GGGAC 3 cut(s) 769, 791, 829
BslI CCNNNNNNNGG 3 cut(s) 499, 583, 920
BsmAI GTCTC 2 cut(s) 89, 302
BsmFI GGGAC 3 cut(s) 769, 791, 829
BsmI GAATGC 2 cut(s) 610, 691
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 2 cut(s) 396, 550
Bsp143I GATC 1 cut(s) 832
BspACI CCGC 2 cut(s) 138, 517
BspCNI CTCAG 3 cut(s) 99, 758, 893
BspFNI CGCG 1 cut(s) 674
BspLI GGNNCC 4 cut(s) 383, 528, 668, 834
BspPI GGATC 2 cut(s) 827, 840
BspT107I GGYRCC 1 cut(s) 666
BspTNI GGTCTC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 138
BsrI ACTGG 4 cut(s) 108, 785, 824, 837
BssECI CCNNGG 2 cut(s) 422, 791
BssMI GATC 1 cut(s) 832
Bst2UI CCWGG 3 cut(s) 219, 792, 921
Bst4CI ACNGT 3 cut(s) 338, 398, 679
Bst6I CTCTTC 1 cut(s) 423
BstC8I GCNNGC 4 cut(s) 95, 123, 289, 559
BstDEI CTNAG 5 cut(s) 86, 552, 745, 766, 880
BstDSI CCRYGG 1 cut(s) 422
BstENI CCTNNNNNAGG 1 cut(s) 918
BstF5I GGATG 2 cut(s) 484, 487
BstFNI CGCG 1 cut(s) 674
BstHHI GCGC 1 cut(s) 15
BstKTI GATC 1 cut(s) 835
BstMAI GTCTC 2 cut(s) 89, 302
BstMBI GATC 1 cut(s) 832
BstMWI GCNNNNNNNGC 2 cut(s) 554, 983
BstNI CCWGG 3 cut(s) 219, 792, 921
BstSCI CCNGG 3 cut(s) 217, 790, 919
BstSLI GKGCMC 2 cut(s) 396, 550
BstUI CGCG 1 cut(s) 674
BstV1I GCAGC 3 cut(s) 163, 187, 421
BstV2I GAAGAC 2 cut(s) 93, 217
BstX2I RGATCY 1 cut(s) 832
BstYI RGATCY 1 cut(s) 832
Bsu36I CCTNAGG 1 cut(s) 552
BtgI CCRYGG 1 cut(s) 422
BtsCI GGATG 2 cut(s) 484, 487
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 2 cut(s) 115, 615
Cac8I GCNNGC 4 cut(s) 95, 123, 289, 559
CfoI GCGC 1 cut(s) 15
Cfr13I GGNCC 2 cut(s) 165, 932
Csp6I GTAC 2 cut(s) 24, 339
CspCI CAANNNNNGTGG 2 cut(s) 876, 911
CviAII CATG 4 cut(s) 66, 499, 514, 848
CviQI GTAC 2 cut(s) 24, 339
DdeI CTNAG 5 cut(s) 86, 552, 745, 766, 880
DpnI GATC 1 cut(s) 834
DpnII GATC 1 cut(s) 832
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
Eco31I GGTCTC 1 cut(s) 89
Eco47I GGWCC 2 cut(s) 165, 932
Eco57I CTGAAG 3 cut(s) 54, 363, 806
Eco81I CCTNAGG 1 cut(s) 552
EcoNI CCTNNNNNAGG 1 cut(s) 918
EcoO109I RGGNCCY 1 cut(s) 932
EcoRII CCWGG 3 cut(s) 217, 790, 919
FaeI CATG 4 cut(s) 69, 502, 517, 851
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 3 cut(s) 769, 791, 829
FatI CATG 4 cut(s) 65, 498, 513, 847
FauI CCCGC 1 cut(s) 510
FblI GTMKAC 1 cut(s) 71
Fnu4HI GCNGC 3 cut(s) 177, 201, 410
FokI GGATG 2 cut(s) 474, 491
Fsp4HI GCNGC 3 cut(s) 177, 201, 410
GlaI GCGC 1 cut(s) 14
GluI GCNGC 3 cut(s) 177, 201, 410
HapII CCGG 1 cut(s) 379
HhaI GCGC 1 cut(s) 15
Hin1II CATG 4 cut(s) 69, 502, 517, 851
Hin6I GCGC 1 cut(s) 13
HinP1I GCGC 1 cut(s) 13
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HindIII AAGCTT 1 cut(s) 285
HinfI GANTC 6 cut(s) 254, 505, 721, 757, 820, 971
HpaII CCGG 1 cut(s) 379
HphI GGTGA 1 cut(s) 691
Hpy166II GTNNAC 2 cut(s) 72, 394
Hpy188I TCNGA 5 cut(s) 302, 578, 748, 883, 992
Hpy188III TCNNGA 2 cut(s) 168, 464
Hpy8I GTNNAC 2 cut(s) 72, 394
Hpy99I CGWCG 4 cut(s) 52, 55, 391, 729
HpyAV CCTTC 2 cut(s) 190, 516
HpyCH4III ACNGT 3 cut(s) 338, 398, 679
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 8 cut(s) 189, 291, 394, 409, 561, 608, 661, 691
HpyF10VI GCNNNNNNNGC 2 cut(s) 554, 983
HpyF3I CTNAG 5 cut(s) 86, 552, 745, 766, 880
HpySE526I ACGT 1 cut(s) 53
Hsp92II CATG 4 cut(s) 69, 502, 517, 851
HspAI GCGC 1 cut(s) 13
Kzo9I GATC 1 cut(s) 832
LmnI GCTCC 2 cut(s) 417, 579
Lsp1109I GCAGC 3 cut(s) 163, 187, 421
MaeII ACGT 1 cut(s) 53
MaeIII GTNAC 1 cut(s) 242
MalI GATC 1 cut(s) 834
MbiI CCGCTC 1 cut(s) 138
MboI GATC 1 cut(s) 832
MboII GAAGA 6 cut(s) 93, 222, 410, 613, 633, 725
MflI RGATCY 1 cut(s) 832
MhlI GDGCHC 2 cut(s) 396, 550
MluCI AATT 4 cut(s) 402, 451, 566, 716
MlyI GAGTC 3 cut(s) 715, 751, 965
MmeI TCCRAC 1 cut(s) 280
MnlI CCTC 7 cut(s) 87, 426, 430, 503, 657, 877, 917
MroXI GAANNNNTTC 1 cut(s) 253
MseI TTAA 4 cut(s) 281, 375, 828, 995
MslI CAYNNNNRTG 2 cut(s) 64, 264
MspI CCGG 1 cut(s) 379
MspR9I CCNGG 3 cut(s) 219, 792, 921
Mva1269I GAATGC 2 cut(s) 610, 691
MvaI CCWGG 3 cut(s) 219, 792, 921
MvnI CGCG 1 cut(s) 674
MwoI GCNNNNNNNGC 2 cut(s) 554, 983
NdeII GATC 1 cut(s) 832
NlaIII CATG 4 cut(s) 69, 502, 517, 851
NlaIV GGNNCC 4 cut(s) 383, 528, 668, 834
PcsI WCGNNNNNNNCGW 2 cut(s) 59, 68
PctI GAATGC 2 cut(s) 610, 691
PdmI GAANNNNTTC 1 cut(s) 253
PfeI GAWTC 3 cut(s) 254, 505, 820
PfoI TCCNGGA 1 cut(s) 919
PkrI GCNGC 3 cut(s) 178, 202, 411
PleI GAGTC 3 cut(s) 715, 751, 965
PpsI GAGTC 3 cut(s) 715, 751, 965
PpuMI RGGWCCY 1 cut(s) 932
PsiI TTATAA 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 932
Psp6I CCWGG 3 cut(s) 217, 790, 919
PspGI CCWGG 3 cut(s) 217, 790, 919
PspN4I GGNNCC 4 cut(s) 383, 528, 668, 834
PspPI GGNCC 2 cut(s) 165, 932
PspPPI RGGWCCY 1 cut(s) 932
PsuI RGATCY 1 cut(s) 832
RsaI GTAC 2 cut(s) 25, 340
RsaNI GTAC 2 cut(s) 24, 339
RseI CAYNNNNRTG 2 cut(s) 64, 264
SalI GTCGAC 1 cut(s) 70
SaqAI TTAA 4 cut(s) 281, 375, 828, 995
SatI GCNGC 3 cut(s) 177, 201, 410
Sau3AI GATC 1 cut(s) 832
Sau96I GGNCC 2 cut(s) 165, 932
SchI GAGTC 3 cut(s) 715, 751, 965
ScrFI CCNGG 3 cut(s) 219, 792, 921
SduI GDGCHC 2 cut(s) 396, 550
SinI GGWCC 2 cut(s) 165, 932
SmiMI CAYNNNNRTG 2 cut(s) 64, 264
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 4 cut(s) 402, 451, 566, 716
SsiI CCGC 2 cut(s) 138, 517
StyD4I CCNGG 3 cut(s) 217, 790, 919
TaaI ACNGT 3 cut(s) 338, 398, 679
TaiI ACGT 1 cut(s) 56
TaqI TCGA 3 cut(s) 47, 71, 349
TaqII GACCGA 1 cut(s) 71
TasI AATT 4 cut(s) 402, 451, 566, 716
TatI WGTACW 1 cut(s) 338
TfiI GAWTC 3 cut(s) 254, 505, 820
Tru1I TTAA 4 cut(s) 281, 375, 828, 995
Tru9I TTAA 4 cut(s) 281, 375, 828, 995
TscAI CASTG 2 cut(s) 115, 615
TseI GCWGC 3 cut(s) 176, 200, 409
TspDTI ATGAA 4 cut(s) 209, 357, 476, 684
TspRI CASTG 2 cut(s) 115, 615
VneI GTGCAC 1 cut(s) 392
VpaK11BI GGWCC 2 cut(s) 165, 932
XagI CCTNNNNNAGG 1 cut(s) 918
XapI RAATTY 1 cut(s) 451
XmiI GTMKAC 1 cut(s) 71
XmnI GAANNNNTTC 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.