Rw2G000020

Bifunctional protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003056
Physical Location & Seq
Forward (+)
65637 .. 67988
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G000020.1

Sequence Viewer

Length: 1029 bp
ATGAAAAAGAAAAACTCGATCGAGTTAATGGGGGTCTGGCGCAGATTGGGTACAAAAGCTGAAGTCTTGTATTCGACGACGTTCTCATCGTCATGGTCGACCCCTCTCGGTCTCAGTCGCCCGCTGTCTTCTGGTAATGGTAATGGGCAGGCTGAAATAATGAGCGGAAAGCCAATCGCAAAAGACATAAGGTCCAGAGTAGCTGCTGAAATATGCAGAATGAAGGCTGCCACAGGAAGACTGCCTGGATTGGCTGTTGTTTTGGTGGGTAACAGAAAGGATTCACACACTTATGTCCATCTCAAGTTAAAAGCTTGCAATCAAGTCGGAATAGTGACTTCTATTGTTCAACTGCCCCAAGACTGTACTGAAGCTCGACTTATTGATATTGTTTCATCTTTTAACCGGAACCCGTCGGTGCACGGTATAATTGTGCAGCTCCCTCTTCCACAGCATTTAGACGAGGAAAAGATTATAAATTTTGTTAGTCCAGAAAAAGATGTGGATGGCTTTCATCCCCTCAACATGGGGAATCTTGCCATGCGGGGAAGGGAACCCTTATTTATCCCTTGTGCCCCTAAGGCTTGCATTGAATTGTTGCTCCGATATGGGGTGGAGATTATTGGGAAGAATGCAGTGGTTATAGGAAGAAGCAAGATTGTTGGGTTATCCACTTCTTTGCTATTGCAGAGGCACCACGTGACTGTTAGCACCTTGCATTCATTCACCAAGAACCCAGAAGAATTGACTCGGCGCGCTGATGTTGTCATCTCAGATGTTGGGACTCCAAACTTAGTTCAATGGGACTGGCTGAAGCCAGGGGCAGTTGTAGTTGATATGGGGACGAATCCAGTTAAGGATCCCAGTAGCCGTCATGGTTTTCGTATTACAGGAGATGTTTGCTACTCGGAGGCAATCAAGGTGGTGTCAGCCATAACTCCTGTTCCAGGAGGAGTAGGACCTGTTGTAATCTCAATGCTCCTCTCTAACACCCTTGACTCTGCTAAAAGAGCTATCGGATTAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

342

Amino Acids

37.03

Weight (kDa)

9.55

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THF_DHG_CYH PF00763 54 - 169 1.4e-39 Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C PF02882 190 - 337 2.8e-49 Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 476
AccB1I GGYRCC 1 cut(s) 693
AccBSI CCGCTC 1 cut(s) 165
AccI GTMKAC 1 cut(s) 98
AccII CGCG 1 cut(s) 756
AciI CCGC 3 cut(s) 122, 165, 544
AclWI GGATC 2 cut(s) 854, 867
AcsI RAATTY 1 cut(s) 478
AcuI CTGAAG 3 cut(s) 81, 390, 833
AcvI CACGTG 1 cut(s) 700
AdeI CACNNNGTG 1 cut(s) 700
AfaI GTAC 2 cut(s) 52, 367
AfiI CCNNNNNNNGG 3 cut(s) 526, 610, 947
AgsI TTSAA 3 cut(s) 350, 593, 800
AhdI GACNNNNNGTC 1 cut(s) 190
AjnI CCWGG 3 cut(s) 244, 817, 946
AluBI AGCT 6 cut(s) 59, 203, 314, 374, 439, 1013
AluI AGCT 6 cut(s) 59, 203, 314, 374, 439, 1013
Alw21I GWGCWC 1 cut(s) 423
Alw26I GTCTC 1 cut(s) 116
Alw44I GTGCAC 1 cut(s) 419
AlwI GGATC 2 cut(s) 854, 867
ApaLI GTGCAC 1 cut(s) 419
ApeKI GCWGC 3 cut(s) 203, 227, 436
ApoI RAATTY 1 cut(s) 478
Asp700I GAANNNNTTC 1 cut(s) 280
AspLEI GCGC 3 cut(s) 42, 756, 758
AspS9I GGNCC 2 cut(s) 192, 959
AsuHPI GGTGA 1 cut(s) 718
AvaII GGWCC 2 cut(s) 192, 959
AxyI CCTNAGG 1 cut(s) 579
BaeGI GKGCMC 2 cut(s) 423, 577
BamHI GGATCC 1 cut(s) 859
BanI GGYRCC 1 cut(s) 693
BbrPI CACGTG 1 cut(s) 700
BbsI GAAGAC 2 cut(s) 120, 244
Bbv12I GWGCWC 1 cut(s) 423
BbvI GCAGC 3 cut(s) 190, 214, 448
BccI CCATC 2 cut(s) 306, 500
BceAI ACGGC 1 cut(s) 855
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 3 cut(s) 246, 819, 948
BcoDI GTCTC 1 cut(s) 116
BglI GCCNNNNNGGC 1 cut(s) 581
BisI GCNGC 3 cut(s) 204, 228, 437
BlsI GCNGC 3 cut(s) 205, 229, 438
Bme1390I CCNGG 3 cut(s) 246, 819, 948
Bme18I GGWCC 2 cut(s) 192, 959
BmeRI GACNNNNNGTC 1 cut(s) 190
BmgT120I GGNCC 2 cut(s) 192, 959
BmiI GGNNCC 4 cut(s) 410, 555, 695, 861
BmrFI CCNGG 3 cut(s) 246, 819, 948
BmrI ACTGGG 1 cut(s) 858
BmuI ACTGGG 1 cut(s) 858
BpiI GAAGAC 2 cut(s) 120, 244
BpuEI CTTGAG 1 cut(s) 287
BsaAI YACGTR 1 cut(s) 700
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 818
BsaWI WCCGGW 1 cut(s) 405
Bsc4I CCNNNNNNNGG 3 cut(s) 526, 610, 947
Bse1I ACTGG 3 cut(s) 812, 851, 864
Bse21I CCTNAGG 1 cut(s) 579
BseBI CCWGG 3 cut(s) 246, 819, 948
BseDI CCNNGG 1 cut(s) 818
BseGI GGATG 2 cut(s) 511, 514
BseLI CCNNNNNNNGG 3 cut(s) 526, 610, 947
BseMII CTCAG 2 cut(s) 127, 786
BseNI ACTGG 3 cut(s) 812, 851, 864
BsePI GCGCGC 1 cut(s) 754
BseRI GAGGAG 2 cut(s) 966, 971
BseSI GKGCMC 2 cut(s) 423, 577
BseXI GCAGC 3 cut(s) 190, 214, 448
BsgI GTGCAG 1 cut(s) 455
Bsh1236I CGCG 1 cut(s) 756
Bsh1285I CGRYCG 1 cut(s) 21
BshNI GGYRCC 1 cut(s) 693
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 423
BsiSI CCGG 1 cut(s) 406
BslFI GGGAC 3 cut(s) 796, 818, 856
BslI CCNNNNNNNGG 3 cut(s) 526, 610, 947
BsmAI GTCTC 1 cut(s) 116
BsmFI GGGAC 3 cut(s) 796, 818, 856
BsmI GAATGC 2 cut(s) 637, 718
Bso31I GGTCTC 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 423, 577
Bsp143I GATC 2 cut(s) 18, 859
BspACI CCGC 3 cut(s) 122, 165, 544
BspCNI CTCAG 2 cut(s) 126, 785
BspFNI CGCG 1 cut(s) 756
BspLI GGNNCC 4 cut(s) 410, 555, 695, 861
BspPI GGATC 2 cut(s) 854, 867
BspT107I GGYRCC 1 cut(s) 693
BspTNI GGTCTC 1 cut(s) 116
BsrBI CCGCTC 1 cut(s) 165
BsrI ACTGG 3 cut(s) 812, 851, 864
BssECI CCNNGG 1 cut(s) 818
BssHII GCGCGC 1 cut(s) 754
BssMI GATC 2 cut(s) 18, 859
Bst2UI CCWGG 3 cut(s) 246, 819, 948
Bst4CI ACNGT 3 cut(s) 365, 425, 706
Bst6I CTCTTC 1 cut(s) 450
BstBAI YACGTR 1 cut(s) 700
BstC8I GCNNGC 5 cut(s) 122, 150, 316, 586, 756
BstDEI CTNAG 4 cut(s) 113, 579, 772, 793
BstENI CCTNNNNNAGG 1 cut(s) 945
BstF5I GGATG 2 cut(s) 511, 514
BstFNI CGCG 1 cut(s) 756
BstHHI GCGC 3 cut(s) 42, 756, 758
BstKTI GATC 2 cut(s) 21, 862
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 2 cut(s) 18, 859
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 2 cut(s) 581, 1010
BstNI CCWGG 3 cut(s) 246, 819, 948
BstSCI CCNGG 3 cut(s) 244, 817, 946
BstSLI GKGCMC 2 cut(s) 423, 577
BstUI CGCG 1 cut(s) 756
BstV1I GCAGC 3 cut(s) 190, 214, 448
BstV2I GAAGAC 2 cut(s) 120, 244
BstX2I RGATCY 1 cut(s) 859
BstYI RGATCY 1 cut(s) 859
Bsu36I CCTNAGG 1 cut(s) 579
BtsCI GGATG 2 cut(s) 511, 514
BtsI GCAGTG 1 cut(s) 642
BtsIMutI CAGTG 1 cut(s) 642
Cac8I GCNNGC 5 cut(s) 122, 150, 316, 586, 756
CfoI GCGC 3 cut(s) 42, 756, 758
Cfr13I GGNCC 2 cut(s) 192, 959
Csp6I GTAC 2 cut(s) 51, 366
CspCI CAANNNNNGTGG 2 cut(s) 903, 938
CviAII CATG 4 cut(s) 93, 526, 541, 875
CviQI GTAC 2 cut(s) 51, 366
DdeI CTNAG 4 cut(s) 113, 579, 772, 793
DpnI GATC 2 cut(s) 20, 861
DpnII GATC 2 cut(s) 18, 859
DraIII CACNNNGTG 1 cut(s) 700
DriI GACNNNNNGTC 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 450
Eam1105I GACNNNNNGTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 450
Eco31I GGTCTC 1 cut(s) 116
Eco47I GGWCC 2 cut(s) 192, 959
Eco57I CTGAAG 3 cut(s) 81, 390, 833
Eco72I CACGTG 1 cut(s) 700
Eco81I CCTNAGG 1 cut(s) 579
EcoNI CCTNNNNNAGG 1 cut(s) 945
EcoO109I RGGNCCY 1 cut(s) 959
EcoRII CCWGG 3 cut(s) 244, 817, 946
FaeI CATG 4 cut(s) 96, 529, 544, 878
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 3 cut(s) 796, 818, 856
FatI CATG 4 cut(s) 92, 525, 540, 874
FauI CCCGC 2 cut(s) 129, 537
FblI GTMKAC 1 cut(s) 98
Fnu4HI GCNGC 3 cut(s) 204, 228, 437
FokI GGATG 2 cut(s) 501, 518
Fsp4HI GCNGC 3 cut(s) 204, 228, 437
GlaI GCGC 3 cut(s) 41, 755, 757
GluI GCNGC 3 cut(s) 204, 228, 437
HapII CCGG 1 cut(s) 406
HhaI GCGC 3 cut(s) 42, 756, 758
Hin1II CATG 4 cut(s) 96, 529, 544, 878
Hin6I GCGC 3 cut(s) 40, 754, 756
HinP1I GCGC 3 cut(s) 40, 754, 756
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HindIII AAGCTT 1 cut(s) 312
HinfI GANTC 6 cut(s) 281, 532, 748, 784, 847, 998
HpaII CCGG 1 cut(s) 406
HphI GGTGA 1 cut(s) 718
Hpy166II GTNNAC 2 cut(s) 99, 421
Hpy188I TCNGA 5 cut(s) 329, 605, 775, 910, 1019
Hpy188III TCNNGA 2 cut(s) 195, 491
Hpy8I GTNNAC 2 cut(s) 99, 421
Hpy99I CGWCG 3 cut(s) 79, 82, 418
HpyAV CCTTC 2 cut(s) 217, 543
HpyCH4III ACNGT 3 cut(s) 365, 425, 706
HpyCH4IV ACGT 2 cut(s) 80, 699
HpyCH4V TGCA 8 cut(s) 216, 318, 421, 436, 588, 635, 688, 718
HpyF10VI GCNNNNNNNGC 2 cut(s) 581, 1010
HpyF3I CTNAG 4 cut(s) 113, 579, 772, 793
HpySE526I ACGT 2 cut(s) 80, 699
Hsp92II CATG 4 cut(s) 96, 529, 544, 878
HspAI GCGC 3 cut(s) 40, 754, 756
Kzo9I GATC 2 cut(s) 18, 859
LmnI GCTCC 3 cut(s) 444, 606, 984
Lsp1109I GCAGC 3 cut(s) 190, 214, 448
MaeII ACGT 2 cut(s) 80, 699
MaeIII GTNAC 3 cut(s) 269, 334, 700
MalI GATC 2 cut(s) 20, 861
MbiI CCGCTC 1 cut(s) 165
MboI GATC 2 cut(s) 18, 859
MboII GAAGA 6 cut(s) 120, 249, 437, 640, 660, 752
MflI RGATCY 1 cut(s) 859
MhlI GDGCHC 2 cut(s) 423, 577
MluCI AATT 4 cut(s) 429, 478, 593, 743
MlyI GAGTC 3 cut(s) 742, 778, 992
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 8 cut(s) 114, 453, 457, 530, 684, 904, 944, 992
MroXI GAANNNNTTC 1 cut(s) 280
MseI TTAA 5 cut(s) 26, 308, 402, 855, 1022
MslI CAYNNNNRTG 2 cut(s) 91, 291
MspA1I CMGCKG 1 cut(s) 124
MspI CCGG 1 cut(s) 406
MspR9I CCNGG 3 cut(s) 246, 819, 948
Mva1269I GAATGC 2 cut(s) 637, 718
MvaI CCWGG 3 cut(s) 246, 819, 948
MvnI CGCG 1 cut(s) 756
MwoI GCNNNNNNNGC 2 cut(s) 581, 1010
NdeII GATC 2 cut(s) 18, 859
NlaIII CATG 4 cut(s) 96, 529, 544, 878
NlaIV GGNNCC 4 cut(s) 410, 555, 695, 861
NmeAIII GCCGAG 1 cut(s) 730
NmuCI GTSAC 2 cut(s) 334, 700
PauI GCGCGC 1 cut(s) 754
PcsI WCGNNNNNNNCGW 2 cut(s) 86, 95
PctI GAATGC 2 cut(s) 637, 718
PdmI GAANNNNTTC 1 cut(s) 280
PfeI GAWTC 3 cut(s) 281, 532, 847
PfoI TCCNGGA 1 cut(s) 946
PkrI GCNGC 3 cut(s) 205, 229, 438
Ple19I CGATCG 1 cut(s) 21
PleI GAGTC 3 cut(s) 742, 778, 992
PmaCI CACGTG 1 cut(s) 700
PmlI CACGTG 1 cut(s) 700
PpsI GAGTC 3 cut(s) 742, 778, 992
Ppu21I YACGTR 1 cut(s) 700
PpuMI RGGWCCY 1 cut(s) 959
PsiI TTATAA 1 cut(s) 476
Psp5II RGGWCCY 1 cut(s) 959
Psp6I CCWGG 3 cut(s) 244, 817, 946
PspCI CACGTG 1 cut(s) 700
PspGI CCWGG 3 cut(s) 244, 817, 946
PspN4I GGNNCC 4 cut(s) 410, 555, 695, 861
PspPI GGNCC 2 cut(s) 192, 959
PspPPI RGGWCCY 1 cut(s) 959
PsuI RGATCY 1 cut(s) 859
PteI GCGCGC 1 cut(s) 754
PvuI CGATCG 1 cut(s) 21
RsaI GTAC 2 cut(s) 52, 367
RsaNI GTAC 2 cut(s) 51, 366
RseI CAYNNNNRTG 2 cut(s) 91, 291
SalI GTCGAC 1 cut(s) 97
SaqAI TTAA 5 cut(s) 26, 308, 402, 855, 1022
SatI GCNGC 3 cut(s) 204, 228, 437
Sau3AI GATC 2 cut(s) 18, 859
Sau96I GGNCC 2 cut(s) 192, 959
SchI GAGTC 3 cut(s) 742, 778, 992
ScrFI CCNGG 3 cut(s) 246, 819, 948
SduI GDGCHC 2 cut(s) 423, 577
SinI GGWCC 2 cut(s) 192, 959
SmiMI CAYNNNNRTG 2 cut(s) 91, 291
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 4 cut(s) 429, 478, 593, 743
SsiI CCGC 3 cut(s) 122, 165, 544
StyD4I CCNGG 3 cut(s) 244, 817, 946
TaaI ACNGT 3 cut(s) 365, 425, 706
TaiI ACGT 2 cut(s) 83, 702
TaqI TCGA 5 cut(s) 17, 21, 74, 98, 376
TaqII GACCGA 1 cut(s) 98
TasI AATT 4 cut(s) 429, 478, 593, 743
TatI WGTACW 1 cut(s) 365
TfiI GAWTC 3 cut(s) 281, 532, 847
Tru1I TTAA 5 cut(s) 26, 308, 402, 855, 1022
Tru9I TTAA 5 cut(s) 26, 308, 402, 855, 1022
TscAI CASTG 1 cut(s) 642
TseFI GTSAC 2 cut(s) 334, 700
TseI GCWGC 3 cut(s) 203, 227, 436
Tsp45I GTSAC 2 cut(s) 334, 700
TspDTI ATGAA 5 cut(s) 17, 236, 384, 503, 711
TspRI CASTG 1 cut(s) 642
VneI GTGCAC 1 cut(s) 419
VpaK11BI GGWCC 2 cut(s) 192, 959
XagI CCTNNNNNAGG 1 cut(s) 945
XapI RAATTY 1 cut(s) 478
XmiI GTMKAC 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.