Rh2DG000400

Bifunctional protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003056
Physical Location & Seq
Forward (+)
58174 .. 60793
2620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG000400.1

Sequence Viewer

Length: 1032 bp
ATGAAAAAGAAAAACTCGATCGAGTTAATGGGGGTCTGGCGCAGATTGGGTACAAAAGCTGAAGTCTTGTATTCGACGACGTTCTCATCGTCATGGTCGACCCCTCTCGGTCTCAGTCGCCCGGTGTCTTCTGCAGCCAGTGGTAATGGGCAGGCTGAAATAATGAGCGGAAAGCCAATCGCAAAAGACATAAGGTCCAGAGTAGCTGCTGAAATATGCAGAATGAAGGCTGCCACAGGAAGACTGCCTGGATTGGCTGTTGTTTTGGTGGGTAACAGAAAGGATTCACACACTTATGTCCATCTGAAGTTAAAAGCTTGCAATCAAGTCGGAATAGTGACTTCTATTGTTCAACTGCCCCAAGACTGTACTGAAGCTCGACTTATTGATATTGTTTCATCTTTTAACCGGAACCCGTCGGTGCACGGTATAATTGTGCAGCTCCCTCTTCCACAGCATTTAGACGAGGAAAAGATTATAAATTTTGTTAGTCCAGAAAAAGATGTGGATGGCTTTCATCCCCTCAACATGGGGAATCTTGCCATGCGGGGAAGGGAACCCTTATTTATCCCTTGTGCCCCTAAGGCTTGCATTGAATTGTTGCTCCGATATGGGGTGGAGATTATTGGGAAGAATGCAGTGGTTATAGGAAGAAGCAAGATTGTTGGGTTATCCACTTCTTTGCTATTGCAGAGGCACCACGCGACTGTTAGCACCTTGCATTCATTAACCAAGAACCCAGAAGAATTGACTCGGCGCGCTGATGTTGTCATCTCAGACGTTGGGACTCCAAACTTTGTTCAATGGGACTGGCTGAAGCCAGGGGCAGCTGTAGTTGATATGGGGACGAATCCAGTTAAGGATCCCAGTAGCCGTCATGGTTTTCGTATTACAGGAGATGTTTGCTACTCGGAGGCAATCAAGGTGGTGTCAGCCATAACTCCTGTTCCAGGAGGAGTAGGACCTGTTGTAATCTCAATGCTCCTCTCTAACACCCTTGACTCTGCTAAAAGAGCTATCGGATTAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00670 ko01100 map00670 map01100
Pfam Domains
Protein Families
THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

343

Amino Acids

37.02

Weight (kDa)

9.55

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
THF_DHG_CYH PF00763 55 - 170 1.4e-39 Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C PF02882 191 - 338 3.5e-49 Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 479
AccB1I GGYRCC 1 cut(s) 696
AccBSI CCGCTC 1 cut(s) 168
AccI GTMKAC 1 cut(s) 98
AccII CGCG 2 cut(s) 704, 759
AciI CCGC 2 cut(s) 168, 547
AclWI GGATC 2 cut(s) 857, 870
AcsI RAATTY 1 cut(s) 481
AcuI CTGAAG 4 cut(s) 81, 326, 393, 836
AfaI GTAC 2 cut(s) 52, 370
AfiI CCNNNNNNNGG 3 cut(s) 529, 613, 950
AgsI TTSAA 3 cut(s) 353, 596, 803
AhdI GACNNNNNGTC 1 cut(s) 193
AjnI CCWGG 3 cut(s) 247, 820, 949
AluBI AGCT 7 cut(s) 59, 206, 317, 377, 442, 830, 1016
AluI AGCT 7 cut(s) 59, 206, 317, 377, 442, 830, 1016
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 1 cut(s) 116
Alw44I GTGCAC 1 cut(s) 422
AlwI GGATC 2 cut(s) 857, 870
ApaLI GTGCAC 1 cut(s) 422
ApeKI GCWGC 5 cut(s) 134, 206, 230, 439, 827
ApoI RAATTY 1 cut(s) 481
Asp700I GAANNNNTTC 1 cut(s) 283
AspLEI GCGC 3 cut(s) 42, 759, 761
AspS9I GGNCC 2 cut(s) 195, 962
AsuC2I CCSGG 1 cut(s) 122
AvaII GGWCC 2 cut(s) 195, 962
AxyI CCTNAGG 1 cut(s) 582
BaeGI GKGCMC 2 cut(s) 426, 580
BamHI GGATCC 1 cut(s) 862
BanI GGYRCC 1 cut(s) 696
BbsI GAAGAC 2 cut(s) 120, 247
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 5 cut(s) 146, 193, 217, 451, 839
BccI CCATC 2 cut(s) 309, 503
BceAI ACGGC 1 cut(s) 858
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BciT130I CCWGG 3 cut(s) 249, 822, 951
BcnI CCSGG 1 cut(s) 122
BcoDI GTCTC 1 cut(s) 116
BfmI CTRYAG 2 cut(s) 132, 831
BglI GCCNNNNNGGC 1 cut(s) 584
BisI GCNGC 5 cut(s) 135, 207, 231, 440, 828
BlsI GCNGC 5 cut(s) 136, 208, 232, 441, 829
Bme1390I CCNGG 4 cut(s) 122, 249, 822, 951
Bme18I GGWCC 2 cut(s) 195, 962
BmeRI GACNNNNNGTC 1 cut(s) 193
BmgT120I GGNCC 2 cut(s) 195, 962
BmiI GGNNCC 4 cut(s) 413, 558, 698, 864
BmrFI CCNGG 4 cut(s) 122, 249, 822, 951
BmrI ACTGGG 1 cut(s) 861
BmuI ACTGGG 1 cut(s) 861
BpiI GAAGAC 2 cut(s) 120, 247
BpuMI CCSGG 1 cut(s) 122
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 821
BsaWI WCCGGW 1 cut(s) 408
Bsc4I CCNNNNNNNGG 3 cut(s) 529, 613, 950
Bse1I ACTGG 4 cut(s) 138, 815, 854, 867
Bse21I CCTNAGG 1 cut(s) 582
BseBI CCWGG 3 cut(s) 249, 822, 951
BseDI CCNNGG 1 cut(s) 821
BseGI GGATG 2 cut(s) 514, 517
BseLI CCNNNNNNNGG 3 cut(s) 529, 613, 950
BseMII CTCAG 2 cut(s) 127, 789
BseNI ACTGG 4 cut(s) 138, 815, 854, 867
BsePI GCGCGC 1 cut(s) 757
BseRI GAGGAG 2 cut(s) 969, 974
BseSI GKGCMC 2 cut(s) 426, 580
BseXI GCAGC 5 cut(s) 146, 193, 217, 451, 839
BsgI GTGCAG 1 cut(s) 458
Bsh1236I CGCG 2 cut(s) 704, 759
Bsh1285I CGRYCG 1 cut(s) 21
BshNI GGYRCC 1 cut(s) 696
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 426
BsiSI CCGG 2 cut(s) 122, 409
BslFI GGGAC 3 cut(s) 799, 821, 859
BslI CCNNNNNNNGG 3 cut(s) 529, 613, 950
BsmAI GTCTC 1 cut(s) 116
BsmFI GGGAC 3 cut(s) 799, 821, 859
BsmI GAATGC 2 cut(s) 640, 721
Bso31I GGTCTC 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 426, 580
Bsp143I GATC 2 cut(s) 18, 862
BspACI CCGC 2 cut(s) 168, 547
BspCNI CTCAG 2 cut(s) 126, 788
BspFNI CGCG 2 cut(s) 704, 759
BspLI GGNNCC 4 cut(s) 413, 558, 698, 864
BspMAI CTGCAG 1 cut(s) 136
BspPI GGATC 2 cut(s) 857, 870
BspT107I GGYRCC 1 cut(s) 696
BspTNI GGTCTC 1 cut(s) 116
BsrBI CCGCTC 1 cut(s) 168
BsrI ACTGG 4 cut(s) 138, 815, 854, 867
BssECI CCNNGG 1 cut(s) 821
BssHII GCGCGC 1 cut(s) 757
BssMI GATC 2 cut(s) 18, 862
Bst2UI CCWGG 3 cut(s) 249, 822, 951
Bst4CI ACNGT 3 cut(s) 368, 428, 709
Bst6I CTCTTC 1 cut(s) 453
BstC8I GCNNGC 4 cut(s) 153, 319, 589, 759
BstDEI CTNAG 3 cut(s) 113, 582, 775
BstENI CCTNNNNNAGG 1 cut(s) 948
BstF5I GGATG 2 cut(s) 514, 517
BstFNI CGCG 2 cut(s) 704, 759
BstHHI GCGC 3 cut(s) 42, 759, 761
BstKTI GATC 2 cut(s) 21, 865
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 2 cut(s) 18, 862
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 2 cut(s) 584, 1013
BstNI CCWGG 3 cut(s) 249, 822, 951
BstSCI CCNGG 4 cut(s) 120, 247, 820, 949
BstSFI CTRYAG 2 cut(s) 132, 831
BstSLI GKGCMC 2 cut(s) 426, 580
BstUI CGCG 2 cut(s) 704, 759
BstV1I GCAGC 5 cut(s) 146, 193, 217, 451, 839
BstV2I GAAGAC 2 cut(s) 120, 247
BstX2I RGATCY 1 cut(s) 862
BstYI RGATCY 1 cut(s) 862
Bsu36I CCTNAGG 1 cut(s) 582
BtsCI GGATG 2 cut(s) 514, 517
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 2 cut(s) 145, 645
Cac8I GCNNGC 4 cut(s) 153, 319, 589, 759
CfoI GCGC 3 cut(s) 42, 759, 761
Cfr13I GGNCC 2 cut(s) 195, 962
Csp6I GTAC 2 cut(s) 51, 369
CspCI CAANNNNNGTGG 2 cut(s) 906, 941
CviAII CATG 4 cut(s) 93, 529, 544, 878
CviQI GTAC 2 cut(s) 51, 369
DdeI CTNAG 3 cut(s) 113, 582, 775
DpnI GATC 2 cut(s) 20, 864
DpnII GATC 2 cut(s) 18, 862
DriI GACNNNNNGTC 1 cut(s) 193
Eam1104I CTCTTC 1 cut(s) 453
Eam1105I GACNNNNNGTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 453
Eco31I GGTCTC 1 cut(s) 116
Eco47I GGWCC 2 cut(s) 195, 962
Eco57I CTGAAG 4 cut(s) 81, 326, 393, 836
Eco81I CCTNAGG 1 cut(s) 582
EcoNI CCTNNNNNAGG 1 cut(s) 948
EcoO109I RGGNCCY 1 cut(s) 962
EcoRII CCWGG 3 cut(s) 247, 820, 949
FaeI CATG 4 cut(s) 96, 532, 547, 881
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FaqI GGGAC 3 cut(s) 799, 821, 859
FatI CATG 4 cut(s) 92, 528, 543, 877
FauI CCCGC 1 cut(s) 540
FblI GTMKAC 1 cut(s) 98
Fnu4HI GCNGC 5 cut(s) 135, 207, 231, 440, 828
FokI GGATG 2 cut(s) 504, 521
Fsp4HI GCNGC 5 cut(s) 135, 207, 231, 440, 828
GlaI GCGC 3 cut(s) 41, 758, 760
GluI GCNGC 5 cut(s) 135, 207, 231, 440, 828
HapII CCGG 2 cut(s) 122, 409
HhaI GCGC 3 cut(s) 42, 759, 761
Hin1II CATG 4 cut(s) 96, 532, 547, 881
Hin6I GCGC 3 cut(s) 40, 757, 759
HinP1I GCGC 3 cut(s) 40, 757, 759
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HindIII AAGCTT 1 cut(s) 315
HinfI GANTC 6 cut(s) 284, 535, 751, 787, 850, 1001
HpaII CCGG 2 cut(s) 122, 409
Hpy166II GTNNAC 2 cut(s) 99, 424
Hpy188I TCNGA 6 cut(s) 306, 332, 608, 778, 913, 1022
Hpy188III TCNNGA 2 cut(s) 198, 494
Hpy8I GTNNAC 2 cut(s) 99, 424
Hpy99I CGWCG 3 cut(s) 79, 82, 421
HpyAV CCTTC 2 cut(s) 220, 546
HpyCH4III ACNGT 3 cut(s) 368, 428, 709
HpyCH4IV ACGT 2 cut(s) 80, 780
HpyCH4V TGCA 9 cut(s) 134, 219, 321, 424, 439, 591, 638, 691, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 584, 1013
HpyF3I CTNAG 3 cut(s) 113, 582, 775
HpySE526I ACGT 2 cut(s) 80, 780
Hsp92II CATG 4 cut(s) 96, 532, 547, 881
HspAI GCGC 3 cut(s) 40, 757, 759
Kzo9I GATC 2 cut(s) 18, 862
LmnI GCTCC 3 cut(s) 447, 609, 987
Lsp1109I GCAGC 5 cut(s) 146, 193, 217, 451, 839
MaeII ACGT 2 cut(s) 80, 780
MaeIII GTNAC 2 cut(s) 272, 337
MalI GATC 2 cut(s) 20, 864
MbiI CCGCTC 1 cut(s) 168
MboI GATC 2 cut(s) 18, 862
MboII GAAGA 6 cut(s) 120, 252, 440, 643, 663, 755
MflI RGATCY 1 cut(s) 862
MhlI GDGCHC 2 cut(s) 426, 580
MluCI AATT 4 cut(s) 432, 481, 596, 746
MlyI GAGTC 3 cut(s) 745, 781, 995
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 8 cut(s) 114, 456, 460, 533, 687, 907, 947, 995
MroXI GAANNNNTTC 1 cut(s) 283
MseI TTAA 6 cut(s) 26, 311, 405, 728, 858, 1025
MslI CAYNNNNRTG 2 cut(s) 91, 294
MspA1I CMGCKG 1 cut(s) 830
MspI CCGG 2 cut(s) 122, 409
MspR9I CCNGG 4 cut(s) 122, 249, 822, 951
Mva1269I GAATGC 2 cut(s) 640, 721
MvaI CCWGG 3 cut(s) 249, 822, 951
MvnI CGCG 2 cut(s) 704, 759
MwoI GCNNNNNNNGC 2 cut(s) 584, 1013
NciI CCSGG 1 cut(s) 122
NdeII GATC 2 cut(s) 18, 862
NlaIII CATG 4 cut(s) 96, 532, 547, 881
NlaIV GGNNCC 4 cut(s) 413, 558, 698, 864
NmeAIII GCCGAG 1 cut(s) 733
NmuCI GTSAC 1 cut(s) 337
PauI GCGCGC 1 cut(s) 757
PcsI WCGNNNNNNNCGW 2 cut(s) 86, 95
PctI GAATGC 2 cut(s) 640, 721
PdmI GAANNNNTTC 1 cut(s) 283
PfeI GAWTC 3 cut(s) 284, 535, 850
PfoI TCCNGGA 1 cut(s) 949
PkrI GCNGC 5 cut(s) 136, 208, 232, 441, 829
Ple19I CGATCG 1 cut(s) 21
PleI GAGTC 3 cut(s) 745, 781, 995
PpsI GAGTC 3 cut(s) 745, 781, 995
PpuMI RGGWCCY 1 cut(s) 962
PsiI TTATAA 1 cut(s) 479
Psp5II RGGWCCY 1 cut(s) 962
Psp6I CCWGG 3 cut(s) 247, 820, 949
PspGI CCWGG 3 cut(s) 247, 820, 949
PspN4I GGNNCC 4 cut(s) 413, 558, 698, 864
PspPI GGNCC 2 cut(s) 195, 962
PspPPI RGGWCCY 1 cut(s) 962
PstI CTGCAG 1 cut(s) 136
PsuI RGATCY 1 cut(s) 862
PteI GCGCGC 1 cut(s) 757
PvuI CGATCG 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 830
RsaI GTAC 2 cut(s) 52, 370
RsaNI GTAC 2 cut(s) 51, 369
RseI CAYNNNNRTG 2 cut(s) 91, 294
SalI GTCGAC 1 cut(s) 97
SaqAI TTAA 6 cut(s) 26, 311, 405, 728, 858, 1025
SatI GCNGC 5 cut(s) 135, 207, 231, 440, 828
Sau3AI GATC 2 cut(s) 18, 862
Sau96I GGNCC 2 cut(s) 195, 962
SchI GAGTC 3 cut(s) 745, 781, 995
ScrFI CCNGG 4 cut(s) 122, 249, 822, 951
SduI GDGCHC 2 cut(s) 426, 580
SfcI CTRYAG 2 cut(s) 132, 831
SinI GGWCC 2 cut(s) 195, 962
SmiMI CAYNNNNRTG 2 cut(s) 91, 294
Sse9I AATT 4 cut(s) 432, 481, 596, 746
SsiI CCGC 2 cut(s) 168, 547
StyD4I CCNGG 4 cut(s) 120, 247, 820, 949
TaaI ACNGT 3 cut(s) 368, 428, 709
TaiI ACGT 2 cut(s) 83, 783
TaqI TCGA 5 cut(s) 17, 21, 74, 98, 379
TaqII GACCGA 1 cut(s) 98
TasI AATT 4 cut(s) 432, 481, 596, 746
TatI WGTACW 1 cut(s) 368
TfiI GAWTC 3 cut(s) 284, 535, 850
Tru1I TTAA 6 cut(s) 26, 311, 405, 728, 858, 1025
Tru9I TTAA 6 cut(s) 26, 311, 405, 728, 858, 1025
TscAI CASTG 2 cut(s) 145, 645
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 5 cut(s) 134, 206, 230, 439, 827
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 5 cut(s) 17, 239, 387, 506, 714
TspRI CASTG 2 cut(s) 145, 645
VneI GTGCAC 1 cut(s) 422
VpaK11BI GGWCC 2 cut(s) 195, 962
XagI CCTNNNNNAGG 1 cut(s) 948
XapI RAATTY 1 cut(s) 481
XmiI GTMKAC 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.