MD03G1142300.v1.1

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014974
Physical Location & Seq
Forward (+)
15132186 .. 15133422
1237 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1142300.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGCGGAAGAGCATCGTTGCCAGGCACAGCAGCTATGCGTCAACAACTGCGGTTTCTTCGGAAGCCCGACGACGCAGAACTTGTGTTCCAAATGCTACCGTGACTTGCAGCTCAAGGAACAACAGGCGGTCGCTCTCAACCAAACTCTCATCTCCTCTTCCTCTTTCGCTTCTCCTTCCTCCTCCTCCTCTCCCTCTCCCTCTCGTCCGTTTTCGTCCCCGCTGATCTCCGTCTCACCCGCGCACAAGGCACGGGTGGAGCGCGTGGTCGAAGCCAAGGAGGAAGAGGAGAAGGAGGCCGCGCCGTCGGCGGGGGCGCAGGCGAACAGGTGCACGACGTGCCGACGGCGCGTGGGGTTGACGGGGTTTAAGTGCAGGTGCGGGATGACGTTCTGTGGGACCCACAGGTACCCGGAGCAGCACGCGTGCGGGTTCGATTTCAGAGGGATGGGGAAGGAGCAGATCGCCAAGGCCAACCCGGTTGTGAAGGCGGAGAAGCTGCATAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

171

Amino Acids

18.6

Weight (kDa)

9.04

Isoelectric Point (pI)

68.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 11 - 34 4.7e-13 A20-like zinc finger
zf-AN1 PF01428 111 - 146 4.3e-10 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014974)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27580 AT2G27580
fragaria_vesca FvH4_3g24650
malus_domestica MD03G1142300.v1.1 MD11G1162800.v1.1
prunus_persica Prupe.6G127400_v2.0.a1
pyrus_communis pycom03g09700
rosa_chinensis RchiOBHm_Chr5g0044211
rosa_laevigata RLG00000034253
rosa_multiflora Rmu_sc0000776.1_g000081 Rmu_sc0002731.1_g000003
rosa_roxburghii Rroxscaffold_1G00036830
rosa_rugosa Rorug05G0211700
rosa_samantha Rh5BG304100 Rh5DG312800
rosa_wichuraiana Rw5G027460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 366
Acc36I ACCTGC 1 cut(s) 366
Acc65I GGTACC 1 cut(s) 408
AccB1I GGYRCC 1 cut(s) 408
AccII CGCG 5 cut(s) 242, 264, 302, 351, 425
AdeI CACNNNGTG 1 cut(s) 339
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 1 cut(s) 311
AflIII ACRYGT 1 cut(s) 423
AjiI CACGTC 1 cut(s) 339
AjnI CCWGG 1 cut(s) 21
AloI GAACNNNNNNTCC 2 cut(s) 71, 103
AluBI AGCT 3 cut(s) 34, 112, 499
AluI AGCT 3 cut(s) 34, 112, 499
Alw21I GWGCWC 1 cut(s) 335
Alw26I GTCTC 1 cut(s) 238
Alw44I GTGCAC 1 cut(s) 331
AoxI GGCC 2 cut(s) 297, 471
ApaLI GTGCAC 1 cut(s) 331
ApeKI GCWGC 4 cut(s) 31, 109, 418, 499
Asp718I GGTACC 1 cut(s) 408
AspLEI GCGC 5 cut(s) 244, 264, 304, 319, 351
AspS9I GGNCC 1 cut(s) 399
AsuC2I CCSGG 2 cut(s) 413, 479
AsuHPI GGTGA 1 cut(s) 228
AvaII GGWCC 1 cut(s) 399
BaeGI GKGCMC 1 cut(s) 335
BanI GGYRCC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 335
BbvI GCAGC 4 cut(s) 43, 121, 430, 486
BccI CCATC 1 cut(s) 442
BceAI ACGGC 2 cut(s) 289, 362
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 2 cut(s) 413, 479
BcoDI GTCTC 1 cut(s) 238
BfuAI ACCTGC 1 cut(s) 366
BisI GCNGC 5 cut(s) 32, 110, 300, 419, 500
BlsI GCNGC 5 cut(s) 33, 111, 301, 420, 501
Bme1390I CCNGG 3 cut(s) 23, 413, 479
Bme18I GGWCC 1 cut(s) 399
BmgBI CACGTC 1 cut(s) 339
BmgT120I GGNCC 1 cut(s) 399
BmiI GGNNCC 3 cut(s) 400, 401, 410
BmrFI CCNGG 3 cut(s) 23, 413, 479
BmsI GCATC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 98
BpuMI CCSGG 2 cut(s) 413, 479
BsaJI CCNNGG 2 cut(s) 276, 468
Bsc4I CCNNNNNNNGG 1 cut(s) 311
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 2 cut(s) 276, 468
BseGI GGATG 2 cut(s) 390, 453
BseLI CCNNNNNNNGG 1 cut(s) 311
BseRI GAGGAG 5 cut(s) 145, 172, 175, 178, 302
BseSI GKGCMC 1 cut(s) 335
BseXI GCAGC 4 cut(s) 43, 121, 430, 486
BsgI GTGCAG 1 cut(s) 394
Bsh1236I CGCG 5 cut(s) 242, 264, 302, 351, 425
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 2 cut(s) 299, 473
BshNI GGYRCC 1 cut(s) 408
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 335
BsiSI CCGG 2 cut(s) 413, 479
BslFI GGGAC 2 cut(s) 202, 412
BslI CCNNNNNNNGG 1 cut(s) 311
BsmAI GTCTC 1 cut(s) 238
BsmBI CGTCTC 1 cut(s) 238
BsmFI GGGAC 2 cut(s) 202, 412
BsnI GGCC 2 cut(s) 299, 473
Bsp1286I GDGCHC 1 cut(s) 335
Bsp143I GATC 2 cut(s) 225, 462
BspANI GGCC 2 cut(s) 299, 473
BspFNI CGCG 5 cut(s) 242, 264, 302, 351, 425
BspLI GGNNCC 3 cut(s) 400, 401, 410
BspMI ACCTGC 1 cut(s) 366
BspQI GCTCTTC 1 cut(s) 3
BspT107I GGYRCC 1 cut(s) 408
BssECI CCNNGG 2 cut(s) 276, 468
BssMI GATC 2 cut(s) 225, 462
BssT1I CCWWGG 2 cut(s) 276, 468
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 3 cut(s) 3, 163, 279
BstAPI GCANNNNNTGC 1 cut(s) 339
BstC8I GCNNGC 3 cut(s) 321, 423, 427
BstF5I GGATG 2 cut(s) 390, 453
BstFNI CGCG 5 cut(s) 242, 264, 302, 351, 425
BstHHI GCGC 5 cut(s) 244, 264, 304, 319, 351
BstKTI GATC 2 cut(s) 228, 465
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 2 cut(s) 225, 462
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 4 cut(s) 248, 308, 339, 348
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 3 cut(s) 21, 411, 477
BstSLI GKGCMC 1 cut(s) 335
BstUI CGCG 5 cut(s) 242, 264, 302, 351, 425
BstV1I GCAGC 4 cut(s) 43, 121, 430, 486
BsuRI GGCC 2 cut(s) 299, 473
BtrI CACGTC 1 cut(s) 339
BtsCI GGATG 2 cut(s) 390, 453
BveI ACCTGC 1 cut(s) 366
Cac8I GCNNGC 3 cut(s) 321, 423, 427
CfoI GCGC 5 cut(s) 244, 264, 304, 319, 351
Cfr13I GGNCC 1 cut(s) 399
CseI GACGC 2 cut(s) 28, 82
Csp6I GTAC 1 cut(s) 409
CviJI RGCY 7 cut(s) 34, 66, 112, 275, 299, 473, 499
CviKI_1 RGCY 7 cut(s) 34, 66, 112, 275, 299, 473, 499
CviQI GTAC 1 cut(s) 409
DpnI GATC 2 cut(s) 227, 464
DpnII GATC 2 cut(s) 225, 462
DraIII CACNNNGTG 1 cut(s) 339
Eam1104I CTCTTC 3 cut(s) 3, 163, 279
EarI CTCTTC 3 cut(s) 3, 163, 279
EciI GGCGGA 2 cut(s) 20, 506
Eco130I CCWWGG 2 cut(s) 276, 468
Eco47I GGWCC 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 2 cut(s) 276, 468
ErhI CCWWGG 2 cut(s) 276, 468
Esp3I CGTCTC 1 cut(s) 238
FaiI YATR 2 cut(s) 37, 504
FaqI GGGAC 2 cut(s) 202, 412
FauI CCCGC 5 cut(s) 228, 247, 304, 374, 422
Fnu4HI GCNGC 5 cut(s) 32, 110, 300, 419, 500
FokI GGATG 2 cut(s) 397, 460
Fsp4HI GCNGC 5 cut(s) 32, 110, 300, 419, 500
GlaI GCGC 5 cut(s) 243, 263, 303, 318, 350
GluI GCNGC 5 cut(s) 32, 110, 300, 419, 500
HaeIII GGCC 2 cut(s) 299, 473
HapII CCGG 2 cut(s) 413, 479
HgaI GACGC 2 cut(s) 28, 82
HhaI GCGC 5 cut(s) 244, 264, 304, 319, 351
Hin6I GCGC 5 cut(s) 242, 262, 302, 317, 349
HinP1I GCGC 5 cut(s) 242, 262, 302, 317, 349
HincII GTYRAC 2 cut(s) 43, 360
HindII GTYRAC 2 cut(s) 43, 360
HpaII CCGG 2 cut(s) 413, 479
HphI GGTGA 1 cut(s) 228
Hpy166II GTNNAC 3 cut(s) 43, 333, 360
Hpy188I TCNGA 2 cut(s) 62, 443
Hpy8I GTNNAC 3 cut(s) 43, 333, 360
Hpy99I CGWCG 5 cut(s) 73, 76, 310, 340, 348
HpyAV CCTTC 4 cut(s) 186, 286, 448, 481
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 2 cut(s) 338, 389
HpyCH4V TGCA 4 cut(s) 109, 333, 375, 502
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 308, 339, 348
HpySE526I ACGT 2 cut(s) 338, 389
HspAI GCGC 5 cut(s) 242, 262, 302, 317, 349
KflI GGGWCCC 1 cut(s) 399
KpnI GGTACC 1 cut(s) 412
Kzo9I GATC 2 cut(s) 225, 462
LguI GCTCTTC 1 cut(s) 3
LmnI GCTCC 3 cut(s) 259, 415, 457
LpnPI CCDG 9 cut(s) 8, 35, 110, 305, 313, 361, 391, 426, 492
Lsp1109I GCAGC 4 cut(s) 43, 121, 430, 486
LweI GCATC 1 cut(s) 22
MaeII ACGT 2 cut(s) 338, 389
MaeIII GTNAC 1 cut(s) 101
MalI GATC 2 cut(s) 227, 464
MboI GATC 2 cut(s) 225, 462
MboII GAAGA 4 cut(s) 20, 49, 150, 296
MhlI GDGCHC 1 cut(s) 335
MluI ACGCGT 1 cut(s) 423
MseI TTAA 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 2 cut(s) 413, 479
MspR9I CCNGG 3 cut(s) 23, 413, 479
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 5 cut(s) 242, 264, 302, 351, 425
MwoI GCNNNNNNNGC 4 cut(s) 248, 308, 339, 348
NciI CCSGG 2 cut(s) 413, 479
NdeII GATC 2 cut(s) 225, 462
NlaIV GGNNCC 3 cut(s) 400, 401, 410
NmuCI GTSAC 1 cut(s) 101
PaqCI CACCTGC 1 cut(s) 366
PciSI GCTCTTC 1 cut(s) 3
PkrI GCNGC 5 cut(s) 33, 111, 301, 420, 501
PpuMI RGGWCCY 1 cut(s) 399
Psp5II RGGWCCY 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 3 cut(s) 400, 401, 410
PspPI GGNCC 1 cut(s) 399
PspPPI RGGWCCY 1 cut(s) 399
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
SapI GCTCTTC 1 cut(s) 3
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 5 cut(s) 32, 110, 300, 419, 500
Sau3AI GATC 2 cut(s) 225, 462
Sau96I GGNCC 1 cut(s) 399
ScrFI CCNGG 3 cut(s) 23, 413, 479
SduI GDGCHC 1 cut(s) 335
SetI ASST 8 cut(s) 36, 114, 332, 341, 380, 392, 410, 501
SfaNI GCATC 1 cut(s) 22
SinI GGWCC 1 cut(s) 399
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
StyD4I CCNGG 3 cut(s) 21, 411, 477
StyI CCWWGG 2 cut(s) 276, 468
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 2 cut(s) 341, 392
TaqI TCGA 2 cut(s) 270, 435
TauI GCSGC 1 cut(s) 302
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 4 cut(s) 31, 109, 418, 499
Tsp45I GTSAC 1 cut(s) 101
TspGWI ACGGA 2 cut(s) 198, 220
VneI GTGCAC 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.