pycom03g09700

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014974
Physical Location & Seq
Forward (+)
8423147 .. 8423653
507 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g09700.1

Sequence Viewer

Length: 507 bp
ATGGCGGAAGAGCATCGTTGCCAGGCACAGCAGCTATGCGTCAACAACTGCGGTTTCTTCGGAAGCCCGACGACGCAGAACTTGTGTTCCAAATGCTACCGTGACTTGCAGCTCAAGGAACAACAGGCGATCGCTCTCAACCAAACTCTCATCTCCTCTTCCTCTTTCGCTTCTCCTTCCTCGTCCTTCTCTCCCTCTCGTCCGTTTTCGTCCCCGCTGAACTCCGTCTCACCCGCGCACAAGGCACGGGTGGAGCGTGTGGTCGAAGCGAAGGAGGAAGAGGCGAAGGAGGCCGCGCCGTCGGCGGGGGCGCAGGCGAACAGGTGCACGACGTGCCGACGGCGCGTGGGGTTGACGGGGTATAAGTGCAGGTGCGGGATGACGTTCTGTGGGACCCACAGGTACCCGGAGCAGCACGCGTGCGGGTTCGATTTCAGAGGGATGGGGAAGGAGCGGATCGCCAAGGCCAACCCGGTTGTGAAGGCGGAGAAGCTGCATAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

169

Amino Acids

18.48

Weight (kDa)

9.23

Isoelectric Point (pI)

60.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 11 - 34 4.6e-13 A20-like zinc finger
zf-AN1 PF01428 109 - 144 8.8e-10 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014974)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27580 AT2G27580
fragaria_vesca FvH4_3g24650
malus_domestica MD03G1142300.v1.1 MD11G1162800.v1.1
prunus_persica Prupe.6G127400_v2.0.a1
pyrus_communis pycom03g09700
rosa_chinensis RchiOBHm_Chr5g0044211
rosa_laevigata RLG00000034253
rosa_multiflora Rmu_sc0000776.1_g000081 Rmu_sc0002731.1_g000003
rosa_roxburghii Rroxscaffold_1G00036830
rosa_rugosa Rorug05G0211700
rosa_samantha Rh5BG304100 Rh5DG312800
rosa_wichuraiana Rw5G027460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 360
Acc65I GGTACC 1 cut(s) 402
AccB1I GGYRCC 1 cut(s) 402
AccBSI CCGCTC 1 cut(s) 454
AccII CGCG 4 cut(s) 236, 296, 345, 419
AclWI GGATC 1 cut(s) 464
AdeI CACNNNGTG 1 cut(s) 333
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 417
AjiI CACGTC 1 cut(s) 333
AjnI CCWGG 1 cut(s) 21
AloI GAACNNNNNNTCC 2 cut(s) 71, 103
AluBI AGCT 3 cut(s) 34, 112, 493
AluI AGCT 3 cut(s) 34, 112, 493
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 1 cut(s) 232
Alw44I GTGCAC 1 cut(s) 325
AlwI GGATC 1 cut(s) 464
AoxI GGCC 2 cut(s) 291, 465
ApaLI GTGCAC 1 cut(s) 325
ApeKI GCWGC 4 cut(s) 31, 109, 412, 493
AsiSI GCGATCGC 1 cut(s) 132
Asp718I GGTACC 1 cut(s) 402
AspLEI GCGC 4 cut(s) 238, 298, 313, 345
AspS9I GGNCC 1 cut(s) 393
AsuC2I CCSGG 2 cut(s) 407, 473
AsuHPI GGTGA 1 cut(s) 222
AvaII GGWCC 1 cut(s) 393
BaeGI GKGCMC 1 cut(s) 329
BanI GGYRCC 1 cut(s) 402
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 4 cut(s) 43, 121, 424, 480
BccI CCATC 1 cut(s) 436
BceAI ACGGC 2 cut(s) 283, 356
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 2 cut(s) 407, 473
BcoDI GTCTC 1 cut(s) 232
BfuAI ACCTGC 1 cut(s) 360
BisI GCNGC 5 cut(s) 32, 110, 294, 413, 494
BlsI GCNGC 5 cut(s) 33, 111, 295, 414, 495
Bme1390I CCNGG 3 cut(s) 23, 407, 473
Bme18I GGWCC 1 cut(s) 393
BmgBI CACGTC 1 cut(s) 333
BmgT120I GGNCC 1 cut(s) 393
BmiI GGNNCC 3 cut(s) 394, 395, 404
BmrFI CCNGG 3 cut(s) 23, 407, 473
BmsI GCATC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 98
BpuMI CCSGG 2 cut(s) 407, 473
BsaJI CCNNGG 1 cut(s) 462
Bsc4I CCNNNNNNNGG 1 cut(s) 305
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 1 cut(s) 462
BseGI GGATG 2 cut(s) 384, 447
BseLI CCNNNNNNNGG 1 cut(s) 305
BseRI GAGGAG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 329
BseXI GCAGC 4 cut(s) 43, 121, 424, 480
BsgI GTGCAG 1 cut(s) 388
Bsh1236I CGCG 4 cut(s) 236, 296, 345, 419
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 2 cut(s) 293, 467
BshNI GGYRCC 1 cut(s) 402
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 329
BsiSI CCGG 2 cut(s) 407, 473
BslFI GGGAC 2 cut(s) 196, 406
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 1 cut(s) 232
BsmBI CGTCTC 1 cut(s) 232
BsmFI GGGAC 2 cut(s) 196, 406
BsnI GGCC 2 cut(s) 293, 467
Bsp1286I GDGCHC 1 cut(s) 329
Bsp143I GATC 2 cut(s) 129, 456
BspANI GGCC 2 cut(s) 293, 467
BspFNI CGCG 4 cut(s) 236, 296, 345, 419
BspLI GGNNCC 3 cut(s) 394, 395, 404
BspMI ACCTGC 1 cut(s) 360
BspPI GGATC 1 cut(s) 464
BspQI GCTCTTC 1 cut(s) 3
BspT107I GGYRCC 1 cut(s) 402
BsrBI CCGCTC 1 cut(s) 454
BssECI CCNNGG 1 cut(s) 462
BssMI GATC 2 cut(s) 129, 456
BssT1I CCWWGG 1 cut(s) 462
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 3 cut(s) 3, 163, 273
BstAPI GCANNNNNTGC 1 cut(s) 333
BstC8I GCNNGC 3 cut(s) 315, 417, 421
BstF5I GGATG 2 cut(s) 384, 447
BstFNI CGCG 4 cut(s) 236, 296, 345, 419
BstHHI GCGC 4 cut(s) 238, 298, 313, 345
BstKTI GATC 2 cut(s) 132, 459
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 2 cut(s) 129, 456
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 5 cut(s) 242, 290, 302, 333, 342
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 3 cut(s) 21, 405, 471
BstSLI GKGCMC 1 cut(s) 329
BstUI CGCG 4 cut(s) 236, 296, 345, 419
BstV1I GCAGC 4 cut(s) 43, 121, 424, 480
BsuRI GGCC 2 cut(s) 293, 467
BtrI CACGTC 1 cut(s) 333
BtsCI GGATG 2 cut(s) 384, 447
BveI ACCTGC 1 cut(s) 360
Cac8I GCNNGC 3 cut(s) 315, 417, 421
CfoI GCGC 4 cut(s) 238, 298, 313, 345
Cfr13I GGNCC 1 cut(s) 393
CseI GACGC 2 cut(s) 28, 82
Csp6I GTAC 1 cut(s) 403
CviJI RGCY 6 cut(s) 34, 66, 112, 293, 467, 493
CviKI_1 RGCY 6 cut(s) 34, 66, 112, 293, 467, 493
CviQI GTAC 1 cut(s) 403
DpnI GATC 2 cut(s) 131, 458
DpnII GATC 2 cut(s) 129, 456
DraIII CACNNNGTG 1 cut(s) 333
Eam1104I CTCTTC 3 cut(s) 3, 163, 273
EarI CTCTTC 3 cut(s) 3, 163, 273
EciI GGCGGA 2 cut(s) 20, 500
Eco130I CCWWGG 1 cut(s) 462
Eco47I GGWCC 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 393
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 462
ErhI CCWWGG 1 cut(s) 462
Esp3I CGTCTC 1 cut(s) 232
FaiI YATR 3 cut(s) 37, 363, 498
FaqI GGGAC 2 cut(s) 196, 406
FauI CCCGC 5 cut(s) 222, 241, 298, 368, 416
Fnu4HI GCNGC 5 cut(s) 32, 110, 294, 413, 494
FokI GGATG 2 cut(s) 391, 454
Fsp4HI GCNGC 5 cut(s) 32, 110, 294, 413, 494
GlaI GCGC 4 cut(s) 237, 297, 312, 344
GluI GCNGC 5 cut(s) 32, 110, 294, 413, 494
HaeIII GGCC 2 cut(s) 293, 467
HapII CCGG 2 cut(s) 407, 473
HgaI GACGC 2 cut(s) 28, 82
HhaI GCGC 4 cut(s) 238, 298, 313, 345
Hin6I GCGC 4 cut(s) 236, 296, 311, 343
HinP1I GCGC 4 cut(s) 236, 296, 311, 343
HincII GTYRAC 2 cut(s) 43, 354
HindII GTYRAC 2 cut(s) 43, 354
HpaII CCGG 2 cut(s) 407, 473
HphI GGTGA 1 cut(s) 222
Hpy166II GTNNAC 3 cut(s) 43, 327, 354
Hpy188I TCNGA 2 cut(s) 62, 437
Hpy8I GTNNAC 3 cut(s) 43, 327, 354
Hpy99I CGWCG 5 cut(s) 73, 76, 304, 334, 342
HpyAV CCTTC 6 cut(s) 186, 196, 265, 280, 442, 475
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 2 cut(s) 332, 383
HpyCH4V TGCA 4 cut(s) 109, 327, 369, 496
HpyF10VI GCNNNNNNNGC 5 cut(s) 242, 290, 302, 333, 342
HpySE526I ACGT 2 cut(s) 332, 383
HspAI GCGC 4 cut(s) 236, 296, 311, 343
KflI GGGWCCC 1 cut(s) 393
KpnI GGTACC 1 cut(s) 406
Kzo9I GATC 2 cut(s) 129, 456
LguI GCTCTTC 1 cut(s) 3
LmnI GCTCC 3 cut(s) 253, 409, 451
LpnPI CCDG 9 cut(s) 8, 35, 110, 299, 307, 355, 385, 420, 486
Lsp1109I GCAGC 4 cut(s) 43, 121, 424, 480
LweI GCATC 1 cut(s) 22
MaeII ACGT 2 cut(s) 332, 383
MaeIII GTNAC 1 cut(s) 101
MalI GATC 2 cut(s) 131, 458
MbiI CCGCTC 1 cut(s) 454
MboI GATC 2 cut(s) 129, 456
MboII GAAGA 4 cut(s) 20, 49, 150, 290
MhlI GDGCHC 1 cut(s) 329
MluI ACGCGT 1 cut(s) 417
MnlI CCTC 8 cut(s) 166, 172, 190, 205, 268, 274, 283, 431
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 2 cut(s) 407, 473
MspR9I CCNGG 3 cut(s) 23, 407, 473
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 4 cut(s) 236, 296, 345, 419
MwoI GCNNNNNNNGC 5 cut(s) 242, 290, 302, 333, 342
NciI CCSGG 2 cut(s) 407, 473
NdeII GATC 2 cut(s) 129, 456
NlaIV GGNNCC 3 cut(s) 394, 395, 404
NmuCI GTSAC 1 cut(s) 101
PaqCI CACCTGC 1 cut(s) 360
PciSI GCTCTTC 1 cut(s) 3
PcsI WCGNNNNNNNCGW 1 cut(s) 253
PkrI GCNGC 5 cut(s) 33, 111, 295, 414, 495
Ple19I CGATCG 1 cut(s) 132
PpuMI RGGWCCY 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 3 cut(s) 394, 395, 404
PspPI GGNCC 1 cut(s) 393
PspPPI RGGWCCY 1 cut(s) 393
PvuI CGATCG 1 cut(s) 132
RgaI GCGATCGC 1 cut(s) 132
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SapI GCTCTTC 1 cut(s) 3
SatI GCNGC 5 cut(s) 32, 110, 294, 413, 494
Sau3AI GATC 2 cut(s) 129, 456
Sau96I GGNCC 1 cut(s) 393
ScrFI CCNGG 3 cut(s) 23, 407, 473
SduI GDGCHC 1 cut(s) 329
SetI ASST 8 cut(s) 36, 114, 326, 335, 374, 386, 404, 495
SfaAI GCGATCGC 1 cut(s) 132
SfaNI GCATC 1 cut(s) 22
SgfI GCGATCGC 1 cut(s) 132
SinI GGWCC 1 cut(s) 393
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
StyD4I CCNGG 3 cut(s) 21, 405, 471
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 2 cut(s) 335, 386
TaqI TCGA 2 cut(s) 264, 429
TauI GCSGC 1 cut(s) 296
TseFI GTSAC 1 cut(s) 101
TseI GCWGC 4 cut(s) 31, 109, 412, 493
Tsp45I GTSAC 1 cut(s) 101
TspGWI ACGGA 2 cut(s) 192, 214
VneI GTGCAC 1 cut(s) 325
VpaK11BI GGWCC 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.