Rw5G027460

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014974
Physical Location & Seq
Forward (+)
40604992 .. 40605498
507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027460.1

Sequence Viewer

Length: 507 bp
ATGGCGGAAGAGCATAGATGCCAGGCGCCGAAGCTGTGCGTGAACAACTGCGGATTCTTCGGGAGCCCAACCACGCAAAACTTGTGCTCCAAATGCTACCGTGATCTGCAGCACAAAGAGCAACAAGCCCTCGCTCTCAATCAAACCTTCATCTCAGCTTCGTCCACCTCTTCTTCTTCTTCTTCTTCGGCTTTCTTCCCTTCGTCGCCGGTCTCGGTCTCGTCGCCGCAGAACGAGGGCGCGGCCGAGGACGCGACGAAGGTAGAGGAGAAGAAGAAGGAGGAGGAAGCGGTGCCCGGGACGGCGGGGAGGCAGGCGAACAGGTGCACGGAGTGCAGGCGGCGCGTGGGGTTGACGGGGTTCAAGTGCAGGTGCGGGATGACGTACTGCGGGACCCACAGGTACCCGGAGAAGCACGAGTGCGGGTTCGACTTCAGGAGCATGGGGAGGGAGCAGATCGCCAAGGCCAATCCGGTCGTGAAGGGCGACAAGCTGCAGAAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

168

Amino Acids

18.45

Weight (kDa)

8.78

Isoelectric Point (pI)

65.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 12 - 34 9.9e-13 A20-like zinc finger
zf-AN1 PF01428 109 - 144 7.7e-10 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014974)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27580 AT2G27580
fragaria_vesca FvH4_3g24650
malus_domestica MD03G1142300.v1.1 MD11G1162800.v1.1
prunus_persica Prupe.6G127400_v2.0.a1
pyrus_communis pycom03g09700
rosa_chinensis RchiOBHm_Chr5g0044211
rosa_laevigata RLG00000034253
rosa_multiflora Rmu_sc0000776.1_g000081 Rmu_sc0002731.1_g000003
rosa_roxburghii Rroxscaffold_1G00036830
rosa_rugosa Rorug05G0211700
rosa_samantha Rh5BG304100 Rh5DG312800
rosa_wichuraiana Rw5G027460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 360
Acc65I GGTACC 1 cut(s) 402
AccB1I GGYRCC 3 cut(s) 25, 292, 402
AccII CGCG 3 cut(s) 242, 254, 345
AcoI YGGCCR 1 cut(s) 243
AcuI CTGAAG 1 cut(s) 418
AcyI GRCGYC 1 cut(s) 26
AdeI CACNNNGTG 1 cut(s) 333
AfaI GTAC 2 cut(s) 386, 404
AgsI TTSAA 1 cut(s) 364
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 3 cut(s) 34, 158, 493
AluI AGCT 3 cut(s) 34, 158, 493
Alw21I GWGCWC 2 cut(s) 89, 329
Alw26I GTCTC 2 cut(s) 217, 223
Alw44I GTGCAC 1 cut(s) 325
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 2 cut(s) 243, 465
ApaLI GTGCAC 1 cut(s) 325
ApeKI GCWGC 2 cut(s) 109, 493
Asp718I GGTACC 1 cut(s) 402
AspLEI GCGC 3 cut(s) 28, 242, 345
AspS9I GGNCC 1 cut(s) 393
AsuC2I CCSGG 3 cut(s) 297, 298, 407
AvaI CYCGRG 1 cut(s) 296
AvaII GGWCC 1 cut(s) 393
BaeGI GKGCMC 2 cut(s) 297, 329
BanI GGYRCC 3 cut(s) 25, 292, 402
BanII GRGCYC 1 cut(s) 68
BauI CACGAG 1 cut(s) 416
Bbv12I GWGCWC 2 cut(s) 89, 329
BbvI GCAGC 2 cut(s) 121, 480
BceAI ACGGC 1 cut(s) 318
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 3 cut(s) 297, 298, 407
BcoDI GTCTC 2 cut(s) 217, 223
BfmI CTRYAG 2 cut(s) 107, 494
BfoI RGCGCY 1 cut(s) 29
BfuAI ACCTGC 1 cut(s) 360
BglII AGATCT 1 cut(s) 501
BisI GCNGC 5 cut(s) 110, 227, 243, 341, 494
BlsI GCNGC 5 cut(s) 111, 228, 244, 342, 495
Bme1390I CCNGG 4 cut(s) 23, 297, 298, 407
Bme18I GGWCC 1 cut(s) 393
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 393
BmiI GGNNCC 6 cut(s) 27, 65, 294, 394, 395, 404
BmrFI CCNGG 4 cut(s) 23, 297, 298, 407
BmsI GCATC 1 cut(s) 8
BpuMI CCSGG 3 cut(s) 297, 298, 407
BsaHI GRCGYC 1 cut(s) 26
BsaI GGTCTC 2 cut(s) 217, 223
BsaJI CCNNGG 3 cut(s) 246, 296, 462
BsaWI WCCGGW 1 cut(s) 472
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bse118I RCCGGY 1 cut(s) 208
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 3 cut(s) 246, 296, 462
BseGI GGATG 1 cut(s) 384
BseMII CTCAG 1 cut(s) 168
BseRI GAGGAG 2 cut(s) 281, 296
BseSI GKGCMC 2 cut(s) 297, 329
BseX3I CGGCCG 1 cut(s) 243
BseXI GCAGC 2 cut(s) 121, 480
BsgI GTGCAG 2 cut(s) 355, 388
Bsh1236I CGCG 3 cut(s) 242, 254, 345
Bsh1285I CGRYCG 2 cut(s) 246, 477
BshFI GGCC 2 cut(s) 245, 467
BshNI GGYRCC 3 cut(s) 25, 292, 402
BsiEI CGRYCG 2 cut(s) 246, 477
BsiHKAI GWGCWC 2 cut(s) 89, 329
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 4 cut(s) 209, 297, 407, 473
BslFI GGGAC 2 cut(s) 313, 406
BsmAI GTCTC 2 cut(s) 217, 223
BsmFI GGGAC 2 cut(s) 313, 406
BsnI GGCC 2 cut(s) 245, 467
Bso31I GGTCTC 2 cut(s) 217, 223
BsoBI CYCGRG 1 cut(s) 296
Bsp1286I GDGCHC 4 cut(s) 68, 89, 297, 329
Bsp143I GATC 3 cut(s) 103, 456, 501
BspANI GGCC 2 cut(s) 245, 467
BspCNI CTCAG 1 cut(s) 167
BspFNI CGCG 3 cut(s) 242, 254, 345
BspLI GGNNCC 6 cut(s) 27, 65, 294, 394, 395, 404
BspMAI CTGCAG 2 cut(s) 111, 498
BspMI ACCTGC 1 cut(s) 360
BspQI GCTCTTC 1 cut(s) 3
BspT107I GGYRCC 3 cut(s) 25, 292, 402
BspTNI GGTCTC 2 cut(s) 217, 223
BsrFI RCCGGY 1 cut(s) 208
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 3 cut(s) 246, 296, 462
BssMI GATC 3 cut(s) 103, 456, 501
BssNI GRCGYC 1 cut(s) 26
BssSI CACGAG 1 cut(s) 416
BssT1I CCWWGG 1 cut(s) 462
Bst2BI CACGAG 1 cut(s) 416
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 2 cut(s) 3, 175
BstACI GRCGYC 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 315, 338
BstDEI CTNAG 1 cut(s) 154
BstF5I GGATG 1 cut(s) 384
BstFNI CGCG 3 cut(s) 242, 254, 345
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 3 cut(s) 28, 242, 345
BstKTI GATC 3 cut(s) 106, 459, 504
BstMAI GTCTC 2 cut(s) 217, 223
BstMBI GATC 3 cut(s) 103, 456, 501
BstMCI CGRYCG 2 cut(s) 246, 477
BstMWI GCNNNNNNNGC 5 cut(s) 93, 118, 251, 333, 342
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 4 cut(s) 21, 295, 296, 405
BstSFI CTRYAG 2 cut(s) 107, 494
BstSLI GKGCMC 2 cut(s) 297, 329
BstUI CGCG 3 cut(s) 242, 254, 345
BstV1I GCAGC 2 cut(s) 121, 480
BstX2I RGATCY 1 cut(s) 501
BstYI RGATCY 1 cut(s) 501
BstZI CGGCCG 1 cut(s) 243
BsuRI GGCC 2 cut(s) 245, 467
BtsCI GGATG 1 cut(s) 384
BveI ACCTGC 1 cut(s) 360
Cac8I GCNNGC 2 cut(s) 315, 338
CfoI GCGC 3 cut(s) 28, 242, 345
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 1 cut(s) 393
Cfr9I CCCGGG 1 cut(s) 296
CseI GACGC 1 cut(s) 260
Csp6I GTAC 2 cut(s) 385, 403
CviAII CATG 1 cut(s) 442
CviJI RGCY 8 cut(s) 34, 66, 128, 158, 191, 245, 467, 493
CviKI_1 RGCY 8 cut(s) 34, 66, 128, 158, 191, 245, 467, 493
CviQI GTAC 2 cut(s) 385, 403
DdeI CTNAG 1 cut(s) 154
DinI GGCGCC 1 cut(s) 27
DpnI GATC 3 cut(s) 105, 458, 503
DpnII GATC 3 cut(s) 103, 456, 501
DraIII CACNNNGTG 1 cut(s) 333
EaeI YGGCCR 1 cut(s) 243
EagI CGGCCG 1 cut(s) 243
Eam1104I CTCTTC 2 cut(s) 3, 175
EarI CTCTTC 2 cut(s) 3, 175
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 462
Eco24I GRGCYC 1 cut(s) 68
Eco31I GGTCTC 2 cut(s) 217, 223
Eco47I GGWCC 1 cut(s) 393
Eco52I CGGCCG 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 418
Eco88I CYCGRG 1 cut(s) 296
EcoO109I RGGNCCY 1 cut(s) 393
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 462
EcoT38I GRGCYC 1 cut(s) 68
EgeI GGCGCC 1 cut(s) 27
EheI GGCGCC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 462
FaeI CATG 1 cut(s) 445
FaiI YATR 2 cut(s) 15, 443
FaqI GGGAC 2 cut(s) 313, 406
FatI CATG 1 cut(s) 441
FauI CCCGC 4 cut(s) 298, 368, 383, 416
Fnu4HI GCNGC 5 cut(s) 110, 227, 243, 341, 494
FokI GGATG 1 cut(s) 391
FriOI GRGCYC 1 cut(s) 68
Fsp4HI GCNGC 5 cut(s) 110, 227, 243, 341, 494
GlaI GCGC 3 cut(s) 27, 241, 344
GluI GCNGC 5 cut(s) 110, 227, 243, 341, 494
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 2 cut(s) 245, 467
HapII CCGG 4 cut(s) 209, 297, 407, 473
HgaI GACGC 1 cut(s) 260
HhaI GCGC 3 cut(s) 28, 242, 345
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 1 cut(s) 445
Hin6I GCGC 3 cut(s) 26, 240, 343
HinP1I GCGC 3 cut(s) 26, 240, 343
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HinfI GANTC 1 cut(s) 54
HpaII CCGG 4 cut(s) 209, 297, 407, 473
Hpy166II GTNNAC 4 cut(s) 43, 165, 327, 354
Hpy188I TCNGA 1 cut(s) 506
Hpy188III TCNNGA 3 cut(s) 61, 436, 478
Hpy8I GTNNAC 4 cut(s) 43, 165, 327, 354
Hpy99I CGWCG 3 cut(s) 208, 226, 259
HpyAV CCTTC 5 cut(s) 157, 210, 253, 271, 475
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 5 cut(s) 109, 327, 336, 369, 496
HpyF10VI GCNNNNNNNGC 5 cut(s) 93, 118, 251, 333, 342
HpyF3I CTNAG 1 cut(s) 154
HpySE526I ACGT 1 cut(s) 383
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 1 cut(s) 445
HspAI GCGC 3 cut(s) 26, 240, 343
KasI GGCGCC 1 cut(s) 25
KflI GGGWCCC 1 cut(s) 393
KpnI GGTACC 1 cut(s) 406
Kzo9I GATC 3 cut(s) 103, 456, 501
LguI GCTCTTC 1 cut(s) 3
LmnI GCTCC 4 cut(s) 63, 92, 438, 451
Lsp1109I GCAGC 2 cut(s) 121, 480
LweI GCATC 1 cut(s) 8
MaeII ACGT 1 cut(s) 383
MalI GATC 3 cut(s) 105, 458, 503
MboI GATC 3 cut(s) 103, 456, 501
MflI RGATCY 1 cut(s) 501
MhlI GDGCHC 4 cut(s) 68, 89, 297, 329
Mly113I GGCGCC 1 cut(s) 26
MnlI CCTC 9 cut(s) 140, 178, 229, 241, 259, 274, 277, 303, 441
MspI CCGG 4 cut(s) 209, 297, 407, 473
MspR9I CCNGG 4 cut(s) 23, 297, 298, 407
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 3 cut(s) 242, 254, 345
MwoI GCNNNNNNNGC 5 cut(s) 93, 118, 251, 333, 342
NarI GGCGCC 1 cut(s) 26
NciI CCSGG 3 cut(s) 297, 298, 407
NdeII GATC 3 cut(s) 103, 456, 501
NlaIII CATG 1 cut(s) 445
NlaIV GGNNCC 6 cut(s) 27, 65, 294, 394, 395, 404
NmeAIII GCCGAG 1 cut(s) 271
PaqCI CACCTGC 1 cut(s) 360
PciSI GCTCTTC 1 cut(s) 3
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PfeI GAWTC 1 cut(s) 54
PkrI GCNGC 5 cut(s) 111, 228, 244, 342, 495
PluTI GGCGCC 1 cut(s) 29
PpuMI RGGWCCY 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 6 cut(s) 27, 65, 294, 394, 395, 404
PspPI GGNCC 1 cut(s) 393
PspPPI RGGWCCY 1 cut(s) 393
PstI CTGCAG 2 cut(s) 111, 498
PsuI RGATCY 1 cut(s) 501
RsaI GTAC 2 cut(s) 386, 404
RsaNI GTAC 2 cut(s) 385, 403
SapI GCTCTTC 1 cut(s) 3
SatI GCNGC 5 cut(s) 110, 227, 243, 341, 494
Sau3AI GATC 3 cut(s) 103, 456, 501
Sau96I GGNCC 1 cut(s) 393
ScrFI CCNGG 4 cut(s) 23, 297, 298, 407
SduI GDGCHC 4 cut(s) 68, 89, 297, 329
SfaNI GCATC 1 cut(s) 8
SfcI CTRYAG 2 cut(s) 107, 494
SfoI GGCGCC 1 cut(s) 27
SinI GGWCC 1 cut(s) 393
SmaI CCCGGG 1 cut(s) 298
SspDI GGCGCC 1 cut(s) 25
StyD4I CCNGG 4 cut(s) 21, 295, 296, 405
StyI CCWWGG 1 cut(s) 462
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 386
TaqI TCGA 1 cut(s) 429
TaqII GACCGA 1 cut(s) 205
TauI GCSGC 3 cut(s) 229, 245, 343
TfiI GAWTC 1 cut(s) 54
TseI GCWGC 2 cut(s) 109, 493
TspDTI ATGAA 1 cut(s) 139
TspGWI ACGGA 1 cut(s) 344
TspMI CCCGGG 1 cut(s) 296
VneI GTGCAC 1 cut(s) 325
VpaK11BI GGWCC 1 cut(s) 393
XmaI CCCGGG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.