Rroxscaffold_1G00036830

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014974
Physical Location & Seq
Reverse (-)
53775974 .. 53776495
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00036830.1

Sequence Viewer

Length: 522 bp
ATGGCGGAAGAGCATAGATGCCAGGCGCCGAAGCTGTGCGTGAACAACTGCGGATTCTTCGGGAGCCCAACCACGCAAAACTTGTGCTCCAAATGCTACCGTGATCTGCAGCACAAAGAGCAACAAGCCCTCGCTCTCAATCAGACCTTCATCTCAACTTCGTCCACCTCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTGGCTTTCTTCCCTTCGTCGCCGGTCTCGGTCTCGTCGCCTCAGAACGAGCCCGTGACCGAGGACGCGACGAAGGTAGAGGAGGAGAAGAAGAAGGAGGAGGAAGCGGTGCCGGCGGCGGGGAGGCAGGCGAACAGGTGCACGGAGTGCAGGCGGCGCGTGGGGTTGACGGGGTTCAAGTGCAGGTGCGGCATGACGTACTGCGGGACCCACAGGTACCCGGAGAAGCACGAGTGCGGGTTCGACTTCAGGAGCATGGGGAGGGAGCAGATCGCCAAGGCCAATCCGGTCGTCAAGGGCGAAAAGCTGCAGAAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

173

Amino Acids

19.1

Weight (kDa)

8.64

Isoelectric Point (pI)

75.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 12 - 34 1e-12 A20-like zinc finger
zf-AN1 PF01428 114 - 149 8.1e-10 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014974)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27580 AT2G27580
fragaria_vesca FvH4_3g24650
malus_domestica MD03G1142300.v1.1 MD11G1162800.v1.1
prunus_persica Prupe.6G127400_v2.0.a1
pyrus_communis pycom03g09700
rosa_chinensis RchiOBHm_Chr5g0044211
rosa_laevigata RLG00000034253
rosa_multiflora Rmu_sc0000776.1_g000081 Rmu_sc0002731.1_g000003
rosa_roxburghii Rroxscaffold_1G00036830
rosa_rugosa Rorug05G0211700
rosa_samantha Rh5BG304100 Rh5DG312800
rosa_wichuraiana Rw5G027460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 375
Acc36I ACCTGC 1 cut(s) 375
Acc65I GGTACC 1 cut(s) 417
AccB1I GGYRCC 3 cut(s) 25, 310, 417
AccII CGCG 2 cut(s) 269, 360
AciI CCGC 9 cut(s) 5, 51, 308, 317, 320, 355, 390, 405, 438
AcuI CTGAAG 1 cut(s) 433
AcyI GRCGYC 1 cut(s) 26
AdeI CACNNNGTG 1 cut(s) 348
AfaI GTAC 2 cut(s) 401, 419
AfiI CCNNNNNNNGG 1 cut(s) 320
AgsI TTSAA 1 cut(s) 379
AjnI CCWGG 1 cut(s) 21
AluBI AGCT 2 cut(s) 34, 508
AluI AGCT 2 cut(s) 34, 508
Alw21I GWGCWC 2 cut(s) 89, 344
Alw26I GTCTC 2 cut(s) 232, 238
Alw44I GTGCAC 1 cut(s) 340
AoxI GGCC 1 cut(s) 480
ApaLI GTGCAC 1 cut(s) 340
ApeKI GCWGC 2 cut(s) 109, 508
Asp718I GGTACC 1 cut(s) 417
AspLEI GCGC 2 cut(s) 28, 360
AspS9I GGNCC 1 cut(s) 408
AsuC2I CCSGG 1 cut(s) 422
AvaII GGWCC 1 cut(s) 408
BaeGI GKGCMC 1 cut(s) 344
BanI GGYRCC 3 cut(s) 25, 310, 417
BanII GRGCYC 2 cut(s) 68, 255
BauI CACGAG 1 cut(s) 431
Bbv12I GWGCWC 2 cut(s) 89, 344
BbvI GCAGC 2 cut(s) 121, 495
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 1 cut(s) 422
BcoDI GTCTC 2 cut(s) 232, 238
BfmI CTRYAG 2 cut(s) 107, 509
BfoI RGCGCY 1 cut(s) 29
BfuAI ACCTGC 1 cut(s) 375
BglII AGATCT 1 cut(s) 516
BisI GCNGC 5 cut(s) 110, 318, 356, 391, 509
BlsI GCNGC 5 cut(s) 111, 319, 357, 392, 510
Bme1390I CCNGG 2 cut(s) 23, 422
Bme18I GGWCC 1 cut(s) 408
BmgT120I GGNCC 1 cut(s) 408
BmiI GGNNCC 6 cut(s) 27, 65, 312, 409, 410, 419
BmrFI CCNGG 2 cut(s) 23, 422
BmsI GCATC 1 cut(s) 8
BpuMI CCSGG 1 cut(s) 422
BsaHI GRCGYC 1 cut(s) 26
BsaI GGTCTC 2 cut(s) 232, 238
BsaJI CCNNGG 2 cut(s) 261, 477
BsaWI WCCGGW 1 cut(s) 487
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 1 cut(s) 320
Bse118I RCCGGY 2 cut(s) 223, 313
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 2 cut(s) 261, 477
BseLI CCNNNNNNNGG 1 cut(s) 320
BseMII CTCAG 1 cut(s) 257
BseRI GAGGAG 4 cut(s) 160, 296, 299, 314
BseSI GKGCMC 1 cut(s) 344
BseXI GCAGC 2 cut(s) 121, 495
BsgI GTGCAG 2 cut(s) 370, 403
Bsh1236I CGCG 2 cut(s) 269, 360
Bsh1285I CGRYCG 1 cut(s) 492
BshFI GGCC 1 cut(s) 482
BshNI GGYRCC 3 cut(s) 25, 310, 417
BsiEI CGRYCG 1 cut(s) 492
BsiHKAI GWGCWC 2 cut(s) 89, 344
BsiSI CCGG 4 cut(s) 224, 314, 422, 488
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 1 cut(s) 320
BsmAI GTCTC 2 cut(s) 232, 238
BsmFI GGGAC 1 cut(s) 421
BsnI GGCC 1 cut(s) 482
Bso31I GGTCTC 2 cut(s) 232, 238
Bsp1286I GDGCHC 4 cut(s) 68, 89, 255, 344
Bsp143I GATC 3 cut(s) 103, 471, 516
BspACI CCGC 9 cut(s) 5, 51, 308, 317, 320, 355, 390, 405, 438
BspANI GGCC 1 cut(s) 482
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 2 cut(s) 269, 360
BspLI GGNNCC 6 cut(s) 27, 65, 312, 409, 410, 419
BspMAI CTGCAG 2 cut(s) 111, 513
BspMI ACCTGC 1 cut(s) 375
BspQI GCTCTTC 1 cut(s) 3
BspT107I GGYRCC 3 cut(s) 25, 310, 417
BspTNI GGTCTC 2 cut(s) 232, 238
BsrFI RCCGGY 2 cut(s) 223, 313
BssAI RCCGGY 2 cut(s) 223, 313
BssECI CCNNGG 2 cut(s) 261, 477
BssMI GATC 3 cut(s) 103, 471, 516
BssNI GRCGYC 1 cut(s) 26
BssSI CACGAG 1 cut(s) 431
BssT1I CCWWGG 1 cut(s) 477
Bst2BI CACGAG 1 cut(s) 431
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 2 cut(s) 3, 178
BstACI GRCGYC 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 348
BstC8I GCNNGC 3 cut(s) 315, 330, 353
BstDEI CTNAG 1 cut(s) 243
BstFNI CGCG 2 cut(s) 269, 360
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 2 cut(s) 28, 360
BstKTI GATC 3 cut(s) 106, 474, 519
BstMAI GTCTC 2 cut(s) 232, 238
BstMBI GATC 3 cut(s) 103, 471, 516
BstMCI CGRYCG 1 cut(s) 492
BstMWI GCNNNNNNNGC 6 cut(s) 93, 118, 314, 348, 357, 390
BstNI CCWGG 1 cut(s) 23
BstSCI CCNGG 2 cut(s) 21, 420
BstSFI CTRYAG 2 cut(s) 107, 509
BstSLI GKGCMC 1 cut(s) 344
BstUI CGCG 2 cut(s) 269, 360
BstV1I GCAGC 2 cut(s) 121, 495
BstX2I RGATCY 1 cut(s) 516
BstYI RGATCY 1 cut(s) 516
BsuRI GGCC 1 cut(s) 482
BveI ACCTGC 1 cut(s) 375
Cac8I GCNNGC 3 cut(s) 315, 330, 353
CfoI GCGC 2 cut(s) 28, 360
Cfr10I RCCGGY 2 cut(s) 223, 313
Cfr13I GGNCC 1 cut(s) 408
CseI GACGC 1 cut(s) 275
Csp6I GTAC 2 cut(s) 400, 418
CviAII CATG 2 cut(s) 394, 457
CviJI RGCY 7 cut(s) 34, 66, 128, 206, 253, 482, 508
CviKI_1 RGCY 7 cut(s) 34, 66, 128, 206, 253, 482, 508
CviQI GTAC 2 cut(s) 400, 418
DdeI CTNAG 1 cut(s) 243
DinI GGCGCC 1 cut(s) 27
DpnI GATC 3 cut(s) 105, 473, 518
DpnII GATC 3 cut(s) 103, 471, 516
DraIII CACNNNGTG 1 cut(s) 348
Eam1104I CTCTTC 2 cut(s) 3, 178
EarI CTCTTC 2 cut(s) 3, 178
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 477
Eco24I GRGCYC 2 cut(s) 68, 255
Eco31I GGTCTC 2 cut(s) 232, 238
Eco47I GGWCC 1 cut(s) 408
Eco57I CTGAAG 1 cut(s) 433
EcoO109I RGGNCCY 1 cut(s) 408
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 1 cut(s) 477
EcoT38I GRGCYC 2 cut(s) 68, 255
EgeI GGCGCC 1 cut(s) 27
EheI GGCGCC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 2 cut(s) 397, 460
FaiI YATR 3 cut(s) 15, 395, 458
FaqI GGGAC 1 cut(s) 421
FatI CATG 2 cut(s) 393, 456
FauI CCCGC 3 cut(s) 313, 398, 431
Fnu4HI GCNGC 5 cut(s) 110, 318, 356, 391, 509
FriOI GRGCYC 2 cut(s) 68, 255
Fsp4HI GCNGC 5 cut(s) 110, 318, 356, 391, 509
GlaI GCGC 2 cut(s) 27, 359
GluI GCNGC 5 cut(s) 110, 318, 356, 391, 509
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 1 cut(s) 482
HapII CCGG 4 cut(s) 224, 314, 422, 488
HgaI GACGC 1 cut(s) 275
HhaI GCGC 2 cut(s) 28, 360
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 2 cut(s) 397, 460
Hin6I GCGC 2 cut(s) 26, 358
HinP1I GCGC 2 cut(s) 26, 358
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HinfI GANTC 1 cut(s) 54
HpaII CCGG 4 cut(s) 224, 314, 422, 488
Hpy166II GTNNAC 4 cut(s) 43, 165, 342, 369
Hpy188I TCNGA 3 cut(s) 144, 246, 521
Hpy188III TCNNGA 2 cut(s) 61, 451
Hpy8I GTNNAC 4 cut(s) 43, 165, 342, 369
Hpy99I CGWCG 3 cut(s) 223, 241, 274
HpyAV CCTTC 4 cut(s) 157, 225, 268, 289
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 398
HpyCH4V TGCA 5 cut(s) 109, 342, 351, 384, 511
HpyF10VI GCNNNNNNNGC 6 cut(s) 93, 118, 314, 348, 357, 390
HpyF3I CTNAG 1 cut(s) 243
HpySE526I ACGT 1 cut(s) 398
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 2 cut(s) 397, 460
HspAI GCGC 2 cut(s) 26, 358
KasI GGCGCC 1 cut(s) 25
KflI GGGWCCC 1 cut(s) 408
KpnI GGTACC 1 cut(s) 421
KroI GCCGGC 1 cut(s) 313
KroNI GCCGGC 1 cut(s) 315
Kzo9I GATC 3 cut(s) 103, 471, 516
LguI GCTCTTC 1 cut(s) 3
LmnI GCTCC 4 cut(s) 63, 92, 453, 466
Lsp1109I GCAGC 2 cut(s) 121, 495
LweI GCATC 1 cut(s) 8
MaeII ACGT 1 cut(s) 398
MaeIII GTNAC 1 cut(s) 256
MalI GATC 3 cut(s) 105, 473, 518
MboI GATC 3 cut(s) 103, 471, 516
MflI RGATCY 1 cut(s) 516
MhlI GDGCHC 4 cut(s) 68, 89, 255, 344
Mly113I GGCGCC 1 cut(s) 26
MroNI GCCGGC 1 cut(s) 313
MspI CCGG 4 cut(s) 224, 314, 422, 488
MspR9I CCNGG 2 cut(s) 23, 422
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 2 cut(s) 269, 360
MwoI GCNNNNNNNGC 6 cut(s) 93, 118, 314, 348, 357, 390
NaeI GCCGGC 1 cut(s) 315
NarI GGCGCC 1 cut(s) 26
NciI CCSGG 1 cut(s) 422
NdeII GATC 3 cut(s) 103, 471, 516
NgoMIV GCCGGC 1 cut(s) 313
NlaIII CATG 2 cut(s) 397, 460
NlaIV GGNNCC 6 cut(s) 27, 65, 312, 409, 410, 419
NmuCI GTSAC 1 cut(s) 256
PaqCI CACCTGC 1 cut(s) 375
PciSI GCTCTTC 1 cut(s) 3
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PdiI GCCGGC 1 cut(s) 315
PfeI GAWTC 1 cut(s) 54
PkrI GCNGC 5 cut(s) 111, 319, 357, 392, 510
PluTI GGCGCC 1 cut(s) 29
PpuMI RGGWCCY 1 cut(s) 408
Psp5II RGGWCCY 1 cut(s) 408
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 6 cut(s) 27, 65, 312, 409, 410, 419
PspPI GGNCC 1 cut(s) 408
PspPPI RGGWCCY 1 cut(s) 408
PstI CTGCAG 2 cut(s) 111, 513
PsuI RGATCY 1 cut(s) 516
RsaI GTAC 2 cut(s) 401, 419
RsaNI GTAC 2 cut(s) 400, 418
SapI GCTCTTC 1 cut(s) 3
SatI GCNGC 5 cut(s) 110, 318, 356, 391, 509
Sau3AI GATC 3 cut(s) 103, 471, 516
Sau96I GGNCC 1 cut(s) 408
ScrFI CCNGG 2 cut(s) 23, 422
SduI GDGCHC 4 cut(s) 68, 89, 255, 344
SetI ASST 9 cut(s) 36, 149, 170, 279, 341, 389, 401, 419, 510
SfaNI GCATC 1 cut(s) 8
SfcI CTRYAG 2 cut(s) 107, 509
SfoI GGCGCC 1 cut(s) 27
SinI GGWCC 1 cut(s) 408
SsiI CCGC 9 cut(s) 5, 51, 308, 317, 320, 355, 390, 405, 438
SspDI GGCGCC 1 cut(s) 25
StyD4I CCNGG 2 cut(s) 21, 420
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 401
TaqI TCGA 1 cut(s) 444
TaqII GACCGA 2 cut(s) 220, 275
TauI GCSGC 3 cut(s) 320, 358, 393
TfiI GAWTC 1 cut(s) 54
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 2 cut(s) 109, 508
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 1 cut(s) 139
TspGWI ACGGA 1 cut(s) 359
VneI GTGCAC 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.