MD03G1148500.v1.1
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007123
Physical Location & Seq
Forward (+)
16519678 .. 16520973
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1148500.v1.1.491

Sequence Viewer

Length: 765 bp
ATGGAGGAGCTACCACCAGGTTATCGCTTCTACCCAACAGAAGAAGAACTGGTCTCCTTCTACCTGCACAACAAGCTCGAAGGGAAGCAAGTAGATACTCGTCGCGTAATCCCGGTTATAGACGTTTACAGCAAAGAGCCATGGGACCTTCCAAAGTTTTCGGGGGAGTTGTGCAATGGAGACAGAGAGCAATGGTTTTTCTTTACACCGAGGCAACGACGAGAAGCTCAAGGAGGGAGGCCCAACAGGACTACTGCCTCTGGGTACTGGAAGGCCACCGGGTCACCTGGCTATGTTTACTCCTCAGATAACAAGGTGATTGGAGTGAAGAAAACCATGGTTTTCTATAAAGACAAAGCTCCAACTGGAAGAAAAACCAAATGGAAGATGAATGTGTATAGAGCCATTGAAGTAGACAACTCAACTAACACTAGTGCTAGTGCTAGCACTAGTACACCCAAGTTGAGGGATGAATTCAGTTTGTGTCGAGTGTACGTTGTATCTGGGAGTGCTCGAGCATTTGACAGACGGCCATTGGAGCCAGTAGAGGCACAACAAAAGTTTAGTAACTCTACAGAAGCAAGTACTTCTACTTTGAAGACAACAATGGTGGACAAAGCAAGCTCATCAGACGAAACTTATTCACATTCCGGAGAGCTTGGTGATCTCCCGGAGACGTCAGCAGGGATTAATAGTAGTAATTGGGAGATTAATGAAGGTTTTGAAGAACCTTTATGGGAATGGGAACAACTAAATTTCCTCTAA

Protein Analysis

255

Amino Acids

28.94

Weight (kDa)

5.66

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 5 - 134 1e-39 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 680
Acc36I ACCTGC 1 cut(s) 72
AccI GTMKAC 1 cut(s) 414
AccII CGCG 1 cut(s) 105
AccIII TCCGGA 1 cut(s) 650
AcoI YGGCCR 1 cut(s) 530
AcsI RAATTY 2 cut(s) 473, 754
AcyI GRCGYC 1 cut(s) 677
AfaI GTAC 4 cut(s) 266, 454, 494, 586
AfiI CCNNNNNNNGG 1 cut(s) 465
AgsI TTSAA 3 cut(s) 410, 598, 725
AhlI ACTAGT 2 cut(s) 431, 449
AjnI CCWGG 2 cut(s) 16, 286
AluBI AGCT 6 cut(s) 10, 76, 227, 359, 624, 658
AluI AGCT 6 cut(s) 10, 76, 227, 359, 624, 658
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 3 cut(s) 58, 174, 668
Ama87I CYCGRG 1 cut(s) 513
Aor13HI TCCGGA 1 cut(s) 650
AoxI GGCC 3 cut(s) 239, 273, 530
ApoI RAATTY 2 cut(s) 473, 754
AseI ATTAAT 2 cut(s) 690, 711
AspS9I GGNCC 2 cut(s) 145, 240
AsuC2I CCSGG 3 cut(s) 113, 280, 671
AsuHPI GGTGA 3 cut(s) 276, 328, 674
AsuNHI GCTAGC 1 cut(s) 443
AvaI CYCGRG 1 cut(s) 513
AvaII GGWCC 1 cut(s) 145
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 1 cut(s) 514
BceAI ACGGC 1 cut(s) 545
BciT130I CCWGG 2 cut(s) 18, 288
BcnI CCSGG 3 cut(s) 113, 280, 671
BcoDI GTCTC 3 cut(s) 58, 174, 668
BcuI ACTAGT 2 cut(s) 431, 449
BfaI CTAG 4 cut(s) 432, 438, 444, 450
BfmI CTRYAG 1 cut(s) 573
BfuAI ACCTGC 1 cut(s) 72
BmcAI AGTACT 1 cut(s) 586
Bme1390I CCNGG 5 cut(s) 18, 113, 280, 288, 671
Bme18I GGWCC 1 cut(s) 145
BmeT110I CYCGRG 1 cut(s) 513
BmgT120I GGNCC 2 cut(s) 145, 240
BmiI GGNNCC 2 cut(s) 146, 540
BmrFI CCNGG 5 cut(s) 18, 113, 280, 288, 671
BmtI GCTAGC 1 cut(s) 447
BpiI GAAGAC 1 cut(s) 605
BpuEI CTTGAG 1 cut(s) 213
BpuMI CCSGG 3 cut(s) 113, 280, 671
BsaHI GRCGYC 1 cut(s) 677
BsaI GGTCTC 1 cut(s) 58
BsaJI CCNNGG 3 cut(s) 140, 209, 336
BsaWI WCCGGW 1 cut(s) 650
Bsc4I CCNNNNNNNGG 1 cut(s) 465
Bse1I ACTGG 4 cut(s) 54, 272, 370, 542
Bse3DI GCAATG 2 cut(s) 181, 197
BseAI TCCGGA 1 cut(s) 650
BseBI CCWGG 2 cut(s) 18, 288
BseDI CCNNGG 3 cut(s) 140, 209, 336
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 1 cut(s) 465
BseMI GCAATG 2 cut(s) 181, 197
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 4 cut(s) 54, 272, 370, 542
BseRI GAGGAG 2 cut(s) 20, 292
BsgI GTGCAG 1 cut(s) 50
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 3 cut(s) 241, 275, 532
BsiHKAI GWGCWC 1 cut(s) 514
BsiHKCI CYCGRG 1 cut(s) 513
BsiSI CCGG 4 cut(s) 113, 279, 651, 671
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 1 cut(s) 465
BsmAI GTCTC 3 cut(s) 58, 174, 668
BsmBI CGTCTC 1 cut(s) 668
BsmFI GGGAC 1 cut(s) 158
BsnI GGCC 3 cut(s) 241, 275, 532
Bso31I GGTCTC 1 cut(s) 58
BsoBI CYCGRG 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 514
Bsp13I TCCGGA 1 cut(s) 650
Bsp143I GATC 1 cut(s) 664
Bsp19I CCATGG 2 cut(s) 140, 336
BspANI GGCC 3 cut(s) 241, 275, 532
BspCNI CTCAG 1 cut(s) 317
BspEI TCCGGA 1 cut(s) 650
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 2 cut(s) 146, 540
BspMI ACCTGC 1 cut(s) 72
BspOI GCTAGC 1 cut(s) 447
BspTNI GGTCTC 1 cut(s) 58
BsrDI GCAATG 2 cut(s) 181, 197
BsrI ACTGG 4 cut(s) 54, 272, 370, 542
BssECI CCNNGG 3 cut(s) 140, 209, 336
BssMI GATC 1 cut(s) 664
BssNI GRCGYC 1 cut(s) 677
BssT1I CCWWGG 2 cut(s) 140, 336
Bst2UI CCWGG 2 cut(s) 18, 288
BstACI GRCGYC 1 cut(s) 677
BstC8I GCNNGC 2 cut(s) 445, 622
BstDEI CTNAG 1 cut(s) 304
BstDSI CCRYGG 2 cut(s) 140, 336
BstEII GGTNACC 1 cut(s) 282
BstF5I GGATG 1 cut(s) 475
BstFNI CGCG 1 cut(s) 105
BstKTI GATC 1 cut(s) 667
BstMAI GTCTC 3 cut(s) 58, 174, 668
BstMBI GATC 1 cut(s) 664
BstMWI GCNNNNNNNGC 2 cut(s) 73, 538
BstNI CCWGG 2 cut(s) 18, 288
BstPI GGTNACC 1 cut(s) 282
BstSCI CCNGG 5 cut(s) 16, 111, 278, 286, 669
BstSFI CTRYAG 1 cut(s) 573
BstUI CGCG 1 cut(s) 105
BstV2I GAAGAC 1 cut(s) 605
BsuRI GGCC 3 cut(s) 241, 275, 532
BtgI CCRYGG 2 cut(s) 140, 336
BtsCI GGATG 1 cut(s) 475
BveI ACCTGC 1 cut(s) 72
Cac8I GCNNGC 2 cut(s) 445, 622
Cfr13I GGNCC 2 cut(s) 145, 240
CsiI ACCWGGT 1 cut(s) 16
Csp6I GTAC 4 cut(s) 265, 453, 493, 585
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 2 cut(s) 141, 337
CviQI GTAC 4 cut(s) 265, 453, 493, 585
DdeI CTNAG 1 cut(s) 304
DpnI GATC 1 cut(s) 666
DpnII GATC 1 cut(s) 664
EaeI YGGCCR 1 cut(s) 530
Eco130I CCWWGG 2 cut(s) 140, 336
Eco31I GGTCTC 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 145
Eco88I CYCGRG 1 cut(s) 513
Eco91I GGTNACC 1 cut(s) 282
EcoO109I RGGNCCY 1 cut(s) 145
EcoO65I GGTNACC 1 cut(s) 282
EcoRI GAATTC 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 16, 286
EcoT14I CCWWGG 2 cut(s) 140, 336
ErhI CCWWGG 2 cut(s) 140, 336
Esp3I CGTCTC 1 cut(s) 668
FaeI CATG 2 cut(s) 144, 340
FaiI YATR 7 cut(s) 119, 142, 294, 338, 348, 399, 736
FaqI GGGAC 1 cut(s) 158
FatI CATG 2 cut(s) 140, 336
FblI GTMKAC 1 cut(s) 414
FokI GGATG 1 cut(s) 482
FspBI CTAG 4 cut(s) 432, 438, 444, 450
HaeIII GGCC 3 cut(s) 241, 275, 532
HapII CCGG 4 cut(s) 113, 279, 651, 671
Hin1I GRCGYC 1 cut(s) 677
Hin1II CATG 2 cut(s) 144, 340
HpaII CCGG 4 cut(s) 113, 279, 651, 671
HphI GGTGA 3 cut(s) 276, 328, 674
Hpy166II GTNNAC 6 cut(s) 127, 298, 415, 455, 493, 613
Hpy188I TCNGA 2 cut(s) 307, 631
Hpy188III TCNNGA 1 cut(s) 651
Hpy8I GTNNAC 6 cut(s) 127, 298, 415, 455, 493, 613
Hpy99I CGWCG 2 cut(s) 105, 222
HpyAV CCTTC 5 cut(s) 67, 74, 158, 265, 710
HpyCH4IV ACGT 3 cut(s) 123, 495, 677
HpyCH4V TGCA 2 cut(s) 67, 174
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 538
HpyF3I CTNAG 1 cut(s) 304
HpySE526I ACGT 3 cut(s) 123, 495, 677
Hsp92I GRCGYC 1 cut(s) 677
Hsp92II CATG 2 cut(s) 144, 340
Kpn2I TCCGGA 1 cut(s) 650
Kzo9I GATC 1 cut(s) 664
LmnI GCTCC 3 cut(s) 7, 364, 538
MabI ACCWGGT 1 cut(s) 16
MaeI CTAG 4 cut(s) 432, 438, 444, 450
MaeII ACGT 3 cut(s) 123, 495, 677
MaeIII GTNAC 2 cut(s) 282, 566
MalI GATC 1 cut(s) 666
MboI GATC 1 cut(s) 664
MboII GAAGA 7 cut(s) 53, 56, 340, 381, 397, 610, 737
MhlI GDGCHC 1 cut(s) 514
MluCI AATT 3 cut(s) 473, 700, 754
MmeI TCCRAC 1 cut(s) 386
MnlI CCTC 7 cut(s) 204, 227, 231, 268, 313, 459, 541
MroI TCCGGA 1 cut(s) 650
MseI TTAA 2 cut(s) 690, 711
MspI CCGG 4 cut(s) 113, 279, 651, 671
MspR9I CCNGG 5 cut(s) 18, 113, 280, 288, 671
MvaI CCWGG 2 cut(s) 18, 288
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 2 cut(s) 73, 538
NciI CCSGG 3 cut(s) 113, 280, 671
NcoI CCATGG 2 cut(s) 140, 336
NdeII GATC 1 cut(s) 664
NheI GCTAGC 1 cut(s) 443
NlaIII CATG 2 cut(s) 144, 340
NlaIV GGNNCC 2 cut(s) 146, 540
NmuCI GTSAC 1 cut(s) 282
PaeR7I CTCGAG 1 cut(s) 513
PfoI TCCNGGA 1 cut(s) 669
PpuMI RGGWCCY 1 cut(s) 145
PshBI ATTAAT 2 cut(s) 690, 711
Psp5II RGGWCCY 1 cut(s) 145
Psp6I CCWGG 2 cut(s) 16, 286
PspEI GGTNACC 1 cut(s) 282
PspGI CCWGG 2 cut(s) 16, 286
PspN4I GGNNCC 2 cut(s) 146, 540
PspPI GGNCC 2 cut(s) 145, 240
PspPPI RGGWCCY 1 cut(s) 145
PspXI VCTCGAGB 1 cut(s) 513
RsaI GTAC 4 cut(s) 266, 454, 494, 586
RsaNI GTAC 4 cut(s) 265, 453, 493, 585
SaqAI TTAA 2 cut(s) 690, 711
Sau3AI GATC 1 cut(s) 664
Sau96I GGNCC 2 cut(s) 145, 240
ScaI AGTACT 1 cut(s) 586
ScrFI CCNGG 5 cut(s) 18, 113, 280, 288, 671
SduI GDGCHC 1 cut(s) 514
SexAI ACCWGGT 1 cut(s) 16
SfcI CTRYAG 1 cut(s) 573
Sfr274I CTCGAG 1 cut(s) 513
SinI GGWCC 1 cut(s) 145
SlaI CTCGAG 1 cut(s) 513
SmlI CTYRAG 2 cut(s) 228, 513
SmoI CTYRAG 2 cut(s) 228, 513
SpeI ACTAGT 2 cut(s) 431, 449
Sse9I AATT 3 cut(s) 473, 700, 754
SspMI CTAG 4 cut(s) 432, 438, 444, 450
StyD4I CCNGG 5 cut(s) 16, 111, 278, 286, 669
StyI CCWWGG 2 cut(s) 140, 336
TaiI ACGT 3 cut(s) 126, 498, 680
TaqI TCGA 3 cut(s) 78, 487, 514
TasI AATT 3 cut(s) 473, 700, 754
TatI WGTACW 2 cut(s) 452, 584
Tru1I TTAA 2 cut(s) 690, 711
Tru9I TTAA 2 cut(s) 690, 711
TseFI GTSAC 1 cut(s) 282
Tsp45I GTSAC 1 cut(s) 282
TspDTI ATGAA 3 cut(s) 404, 486, 729
VpaK11BI GGWCC 1 cut(s) 145
VspI ATTAAT 2 cut(s) 690, 711
XapI RAATTY 2 cut(s) 473, 754
XhoI CTCGAG 1 cut(s) 513
XmiI GTMKAC 1 cut(s) 414
XspI CTAG 4 cut(s) 432, 438, 444, 450
ZraI GACGTC 1 cut(s) 678
ZrmI AGTACT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.