Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0012760

Physical Location & Seq

Genomic Coordinates Forward (+)

16609996 .. 16611485

Gene Structure 1490 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1149400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 654 bp

ATGGAATCGAAAAAGAGTGGAGAAGTAGTAGCCATTAGTACCGGGGAAAAGGCGAATTACAGTGCCAAAGGAAAGGCTGCTGAAGGTGCTCCTCCTCCTGCTGCTGCTGCGGTCACCGCAAAAGTTACCAAAATCAGGACTATGGGAGGAGGATGGAAAAAGGGTCTGGCGATCATAGACATTGTGCTGCGGCTCGGTGCCGGTTCAGCTGCTGTTGCTGCGGCTTACACTGCAGGGAATACTGAACAGATTCTTCCTTTCTTCACACAGTTCTTCCAGTTCCATGCTCAGTACAATGATCTTCCAACTTTCACGTTTTTTGTTGTTGCAAATGCAGTAGCTGTGGGATACATAGGGTTCTCCTTGCCCTTCTCTATAATCTGCGTTATTCGACCTCATGCAATAGGGCCAAGGCTACTACTCGTTATCCTTGACACAGTGATGGTTGCTCTAATCATAGCTGCCGATGGCGCTTCATCTGCAATTGTGTACTTGGCGCATAACGGGAACGTGAACGCAAACTGGCTTGCTATTTGCAGGCAATACAACGACTTCTGCCAGGCACTCAGTGGGGCTCTTGTATCTTCATTTGTCGCAGCAGTCTTTTTTATGTTTCTGGTTGTGAATTCTACTTTCGCTCTTAAAAGAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0007043 GO:0008150 GO:0009505 GO:0009531 GO:0009987 GO:0016020 GO:0016043 GO:0022607 GO:0030312 GO:0034329 GO:0034330 GO:0042545 GO:0042802 GO:0042803 GO:0044085 GO:0044426 GO:0044462 GO:0044464 GO:0045216 GO:0045229 GO:0046983 GO:0048037 GO:0048226 GO:0051536 GO:0051540 GO:0071554 GO:0071555 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF588

Protein Analysis

218

Amino Acids

22.88

Weight (kDa)

9.45

Isoelectric Point (pI)

18.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CASP_dom	PF04535	54 - 202	1.7e-40	Casparian strip membrane protein domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0012760)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27370
fragaria_vesca	FvH4_3g25570
malus_domestica	MD03G1149400.v1.1 MD11G1168200.v1.1
prunus_persica	Prupe.6G135300_v2.0.a1
pyrus_communis	pycom03g10330 pycom11g14050
rosa_chinensis	RchiOBHm_Chr5g0046421
rosa_laevigata	RLG00000034409 RLG00000034410
rosa_multiflora	Rmu_sc0003878.1_g000049
rosa_roxburghii	Rroxscaffold_1G00034980
rosa_rugosa	Rorug05G0227900
rosa_samantha	Rh5AG309100 Rh5BG317300 Rh5CG343200 Rh5DG327500
rosa_wichuraiana	Rw0G021520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	197
AciI	CCGC	4 cut(s)	110, 117, 190, 221
AcsI	RAATTY	1 cut(s)	625
AcuI	CTGAAG	1 cut(s)	102
AdeI	CACNNNGTG	1 cut(s)	569
AfaI	GTAC	3 cut(s)	40, 293, 491
AfiI	CCNNNNNNNGG	1 cut(s)	135
AjnI	CCWGG	1 cut(s)	558
AluBI	AGCT	3 cut(s)	209, 341, 461
AluI	AGCT	3 cut(s)	209, 341, 461
Alw21I	GWGCWC	1 cut(s)	91
AlwNI	CAGNNNCTG	2 cut(s)	212, 341
AoxI	GGCC	1 cut(s)	407
ApeKI	GCWGC	9 cut(s)	77, 101, 104, 107, 187, 209, 218, 461, 596
ApoI	RAATTY	1 cut(s)	625
Asp700I	GAANNNNTTC	1 cut(s)	249
AspLEI	GCGC	2 cut(s)	473, 499
AspS9I	GGNCC	1 cut(s)	407
AsuC2I	CCSGG	1 cut(s)	43
AsuHPI	GGTGA	1 cut(s)	106
BanI	GGYRCC	1 cut(s)	197
BanII	GRGCYC	1 cut(s)	577
Bbv12I	GWGCWC	1 cut(s)	91
BbvI	GCAGC	9 cut(s)	64, 88, 91, 94, 174, 196, 205, 448, 608
BccI	CCATC	3 cut(s)	147, 436, 461
BciT130I	CCWGG	1 cut(s)	560
BciVI	GTATCC	1 cut(s)	341
BcnI	CCSGG	1 cut(s)	43
BfmI	CTRYAG	1 cut(s)	231
BfoI	RGCGCY	1 cut(s)	474
BfuI	GTATCC	1 cut(s)	341
Bme1390I	CCNGG	2 cut(s)	43, 560
BmgT120I	GGNCC	1 cut(s)	407
BmiI	GGNNCC	1 cut(s)	199
BmrFI	CCNGG	2 cut(s)	43, 560
BpuMI	CCSGG	1 cut(s)	43
BsaJI	CCNNGG	2 cut(s)	42, 410
BsaXI	ACNNNNNCTCC	2 cut(s)	12, 42
Bsc4I	CCNNNNNNNGG	1 cut(s)	135
Bse118I	RCCGGY	1 cut(s)	200
Bse1I	ACTGG	2 cut(s)	277, 527
BseBI	CCWGG	1 cut(s)	560
BseDI	CCNNGG	2 cut(s)	42, 410
BseGI	GGATG	1 cut(s)	158
BseLI	CCNNNNNNNGG	1 cut(s)	135
BseMII	CTCAG	2 cut(s)	302, 580
BseNI	ACTGG	2 cut(s)	277, 527
BseRI	GAGGAG	3 cut(s)	81, 84, 162
BseXI	GCAGC	9 cut(s)	64, 88, 91, 94, 174, 196, 205, 448, 608
BshFI	GGCC	1 cut(s)	409
BshNI	GGYRCC	1 cut(s)	197
BsiHKAI	GWGCWC	1 cut(s)	91
BsiSI	CCGG	2 cut(s)	42, 201
BslI	CCNNNNNNNGG	1 cut(s)	135
BsnI	GGCC	1 cut(s)	409
Bsp1286I	GDGCHC	2 cut(s)	91, 577
Bsp143I	GATC	2 cut(s)	171, 298
BspACI	CCGC	4 cut(s)	110, 117, 190, 221
BspANI	GGCC	1 cut(s)	409
BspCNI	CTCAG	2 cut(s)	301, 579
BspLI	GGNNCC	1 cut(s)	199
BspMAI	CTGCAG	1 cut(s)	235
BspT107I	GGYRCC	1 cut(s)	197
BsrFI	RCCGGY	1 cut(s)	200
BsrI	ACTGG	2 cut(s)	277, 527
BssAI	RCCGGY	1 cut(s)	200
BssECI	CCNNGG	2 cut(s)	42, 410
BssMI	GATC	2 cut(s)	171, 298
BssT1I	CCWWGG	1 cut(s)	410
Bst2UI	CCWGG	1 cut(s)	560
Bst4CI	ACNGT	3 cut(s)	62, 270, 439
BstC8I	GCNNGC	2 cut(s)	528, 539
BstDEI	CTNAG	2 cut(s)	288, 566
BstEII	GGTNACC	1 cut(s)	112
BstF5I	GGATG	1 cut(s)	158
BstH2I	RGCGCY	1 cut(s)	474
BstHHI	GCGC	2 cut(s)	473, 499
BstKTI	GATC	2 cut(s)	174, 301
BstMBI	GATC	2 cut(s)	171, 298
BstMWI	GCNNNNNNNGC	9 cut(s)	86, 107, 116, 206, 215, 218, 230, 470, 479
BstNI	CCWGG	1 cut(s)	560
BstPI	GGTNACC	1 cut(s)	112
BstSCI	CCNGG	2 cut(s)	41, 558
BstSFI	CTRYAG	1 cut(s)	231
BstV1I	GCAGC	9 cut(s)	64, 88, 91, 94, 174, 196, 205, 448, 608
BsuI	GTATCC	1 cut(s)	341
BsuRI	GGCC	1 cut(s)	409
BtsCI	GGATG	1 cut(s)	158
BtsI	GCAGTG	1 cut(s)	228
BtsIMutI	CAGTG	4 cut(s)	67, 228, 444, 574
Cac8I	GCNNGC	2 cut(s)	528, 539
CaiI	CAGNNNCTG	2 cut(s)	212, 341
CfoI	GCGC	2 cut(s)	473, 499
Cfr10I	RCCGGY	1 cut(s)	200
Cfr13I	GGNCC	1 cut(s)	407
Csp6I	GTAC	3 cut(s)	39, 292, 490
CviAII	CATG	2 cut(s)	284, 398
CviQI	GTAC	3 cut(s)	39, 292, 490
DdeI	CTNAG	2 cut(s)	288, 566
DpnI	GATC	2 cut(s)	173, 300
DpnII	GATC	2 cut(s)	171, 298
DraIII	CACNNNGTG	1 cut(s)	569
Eco130I	CCWWGG	1 cut(s)	410
Eco24I	GRGCYC	1 cut(s)	577
Eco57I	CTGAAG	1 cut(s)	102
Eco91I	GGTNACC	1 cut(s)	112
EcoO65I	GGTNACC	1 cut(s)	112
EcoRI	GAATTC	1 cut(s)	625
EcoRII	CCWGG	1 cut(s)	558
EcoT14I	CCWWGG	1 cut(s)	410
EcoT38I	GRGCYC	1 cut(s)	577
ErhI	CCWWGG	1 cut(s)	410
FaeI	CATG	2 cut(s)	287, 401
FaiI	YATR	9 cut(s)	143, 176, 285, 353, 377, 399, 458, 501, 611
FatI	CATG	2 cut(s)	283, 397
FokI	GGATG	1 cut(s)	165
FriOI	GRGCYC	1 cut(s)	577
GlaI	GCGC	2 cut(s)	472, 498
HaeII	RGCGCY	1 cut(s)	474
HaeIII	GGCC	1 cut(s)	409
HapII	CCGG	2 cut(s)	42, 201
HhaI	GCGC	2 cut(s)	473, 499
Hin1II	CATG	2 cut(s)	287, 401
Hin6I	GCGC	2 cut(s)	471, 497
HinP1I	GCGC	2 cut(s)	471, 497
HinfI	GANTC	2 cut(s)	5, 250
HpaII	CCGG	2 cut(s)	42, 201
HphI	GGTGA	1 cut(s)	106
Hpy166II	GTNNAC	2 cut(s)	490, 514
Hpy188III	TCNNGA	1 cut(s)	136
Hpy8I	GTNNAC	2 cut(s)	490, 514
HpyAV	CCTTC	2 cut(s)	77, 379
HpyCH4III	ACNGT	3 cut(s)	62, 270, 439
HpyCH4IV	ACGT	2 cut(s)	314, 510
HpyCH4V	TGCA	6 cut(s)	233, 329, 335, 401, 482, 537
HpyF10VI	GCNNNNNNNGC	9 cut(s)	86, 107, 116, 206, 215, 218, 230, 470, 479
HpyF3I	CTNAG	2 cut(s)	288, 566
HpySE526I	ACGT	2 cut(s)	314, 510
Hsp92II	CATG	2 cut(s)	287, 401
HspAI	GCGC	2 cut(s)	471, 497
Kzo9I	GATC	2 cut(s)	171, 298
LmnI	GCTCC	1 cut(s)	94
Lsp1109I	GCAGC	9 cut(s)	64, 88, 91, 94, 174, 196, 205, 448, 608
MaeII	ACGT	2 cut(s)	314, 510
MaeIII	GTNAC	2 cut(s)	112, 124
MalI	GATC	2 cut(s)	173, 300
MboI	GATC	2 cut(s)	171, 298
MboII	GAAGA	5 cut(s)	245, 253, 265, 293, 576
MfeI	CAATTG	1 cut(s)	483
MhlI	GDGCHC	2 cut(s)	91, 577
MluCI	AATT	3 cut(s)	55, 483, 625
MmeI	TCCRAC	1 cut(s)	329
MnlI	CCTC	5 cut(s)	102, 105, 140, 143, 405
MroXI	GAANNNNTTC	1 cut(s)	249
MseI	TTAA	1 cut(s)	642
MslI	CAYNNNNRTG	1 cut(s)	440
MspA1I	CMGCKG	1 cut(s)	209
MspI	CCGG	2 cut(s)	42, 201
MspR9I	CCNGG	2 cut(s)	43, 560
MunI	CAATTG	1 cut(s)	483
MvaI	CCWGG	1 cut(s)	560
MwoI	GCNNNNNNNGC	9 cut(s)	86, 107, 116, 206, 215, 218, 230, 470, 479
NciI	CCSGG	1 cut(s)	43
NdeII	GATC	2 cut(s)	171, 298
NlaIII	CATG	2 cut(s)	287, 401
NlaIV	GGNNCC	1 cut(s)	199
NmuCI	GTSAC	1 cut(s)	112
PdmI	GAANNNNTTC	1 cut(s)	249
PfeI	GAWTC	2 cut(s)	5, 250
Psp6I	CCWGG	1 cut(s)	558
PspEI	GGTNACC	1 cut(s)	112
PspGI	CCWGG	1 cut(s)	558
PspN4I	GGNNCC	1 cut(s)	199
PspPI	GGNCC	1 cut(s)	407
PstI	CTGCAG	1 cut(s)	235
PstNI	CAGNNNCTG	2 cut(s)	212, 341
PvuII	CAGCTG	1 cut(s)	209
RsaI	GTAC	3 cut(s)	40, 293, 491
RsaNI	GTAC	3 cut(s)	39, 292, 490
RseI	CAYNNNNRTG	1 cut(s)	440
SaqAI	TTAA	1 cut(s)	642
Sau3AI	GATC	2 cut(s)	171, 298
Sau96I	GGNCC	1 cut(s)	407
ScrFI	CCNGG	2 cut(s)	43, 560
SduI	GDGCHC	2 cut(s)	91, 577
SetI	ASST	7 cut(s)	88, 211, 317, 343, 397, 463, 513
SfcI	CTRYAG	1 cut(s)	231
SmiMI	CAYNNNNRTG	1 cut(s)	440
Sse9I	AATT	3 cut(s)	55, 483, 625
SsiI	CCGC	4 cut(s)	110, 117, 190, 221
StyD4I	CCNGG	2 cut(s)	41, 558
StyI	CCWWGG	1 cut(s)	410
TaaI	ACNGT	3 cut(s)	62, 270, 439
TaiI	ACGT	2 cut(s)	317, 513
TaqI	TCGA	2 cut(s)	8, 391
TasI	AATT	3 cut(s)	55, 483, 625
TatI	WGTACW	2 cut(s)	291, 489
TauI	GCSGC	2 cut(s)	193, 224
TfiI	GAWTC	2 cut(s)	5, 250
Tru1I	TTAA	1 cut(s)	642
Tru9I	TTAA	1 cut(s)	642
TscAI	CASTG	4 cut(s)	67, 235, 444, 574
TseFI	GTSAC	1 cut(s)	112
TseI	GCWGC	9 cut(s)	77, 101, 104, 107, 187, 209, 218, 461, 596
Tsp45I	GTSAC	1 cut(s)	112
TspDTI	ATGAA	2 cut(s)	465, 576
TspRI	CASTG	4 cut(s)	67, 235, 444, 574
XapI	RAATTY	1 cut(s)	625
XcmI	CCANNNNNNNNNTGG	1 cut(s)	566
XmnI	GAANNNNTTC	1 cut(s)	249

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.