RLG00000034409

Regulates membrane-cell wall junctions and localized cell wall deposition. Required for establishment of the Casparian strip membrane domain (CSD) and the subsequent formation of Casparian strips, a cell wall modification of the root endodermis that determines an apoplastic barrier between the intraorganismal apoplasm and the extraorganismal apoplasm and prevents lateral diffusion (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012760
Physical Location & Seq
Reverse (-)
42266959 .. 42267848
890 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034409

Sequence Viewer

Length: 627 bp
ATGGACTCGAAAGAGAGTGAAAACATAGCAGTTACTGATGCAAAGGCCGCGGCCAAAGGTAAGGCTGCAGAAGGTGCTCCTCCTACTGCTGTCGTCGCAATGAAAACCAAAACACAGACTAGAAGAGGATCCAAGAAAGGACTAGCAATCATAGACTTGGTCCTCAGGCTTGGTGCCAGTTCGGCTGCTGTTGCTGCTGCTTACAGTCCATTAAGCGCTGAGCAGATTCTTCCCTTCTTCACACAGTTCCTCCAGTTCCATGCTGAATACAATGATCTCCCGACTTTCACGTTTTTTGTCGTTGCAAATGCGATTGCTGTTGGATATATTGGCCTCTCCATGCCCTTCTCTATTGTATGCATCATTCGACCACGTGCAATAGGGCCGAGGCTTCTACTAGTTATCTGTGACACGGTGATGATTGCTCTAGTGGTAGCTGCTGCCGGAGCTTCTTCTGCGATTATGTACTTGGCTCATAACGGAAATTTGGACTCGAACTGGCTTGCTATTTGTATGCAATATACTAACTTTTGCCAGGGACTTAGTGGGGCTGTTGTGGCTTCATTTGTCGCTGCGGTCTTTTTCATGTTGCTGGTTGTGAATTCTACTTTTGCTCTTAAGAAGTAA

Protein Analysis

209

Amino Acids

21.94

Weight (kDa)

9.12

Isoelectric Point (pI)

29.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 46 - 194 1.8e-40 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012760)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27370
fragaria_vesca FvH4_3g25570
malus_domestica MD03G1149400.v1.1 MD11G1168200.v1.1
prunus_persica Prupe.6G135300_v2.0.a1
pyrus_communis pycom03g10330 pycom11g14050
rosa_chinensis RchiOBHm_Chr5g0046421
rosa_laevigata RLG00000034409 RLG00000034410
rosa_multiflora Rmu_sc0003878.1_g000049
rosa_roxburghii Rroxscaffold_1G00034980
rosa_rugosa Rorug05G0227900
rosa_samantha Rh5AG309100 Rh5BG317300 Rh5CG343200 Rh5DG327500
rosa_wichuraiana Rw0G021520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AccII CGCG 1 cut(s) 50
AciI CCGC 3 cut(s) 48, 50, 575
AclWI GGATC 2 cut(s) 123, 136
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 2 cut(s) 484, 601
AcvI CACGTG 1 cut(s) 374
AfaI GTAC 1 cut(s) 467
AfeI AGCGCT 1 cut(s) 217
AflII CTTAAG 1 cut(s) 617
AhlI ACTAGT 1 cut(s) 397
AjnI CCWGG 1 cut(s) 534
AluBI AGCT 2 cut(s) 437, 449
AluI AGCT 2 cut(s) 437, 449
Alw21I GWGCWC 1 cut(s) 79
AlwI GGATC 2 cut(s) 123, 136
AlwNI CAGNNNCTG 1 cut(s) 35
Aor51HI AGCGCT 1 cut(s) 217
AoxI GGCC 4 cut(s) 45, 51, 331, 383
ApeKI GCWGC 7 cut(s) 65, 185, 194, 197, 437, 440, 572
ApoI RAATTY 2 cut(s) 484, 601
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 2 cut(s) 160, 383
AsuHPI GGTGA 1 cut(s) 427
AvaII GGWCC 1 cut(s) 160
AxyI CCTNAGG 1 cut(s) 164
BamHI GGATCC 1 cut(s) 128
BanI GGYRCC 1 cut(s) 173
BbrPI CACGTG 1 cut(s) 374
Bbv12I GWGCWC 1 cut(s) 79
BbvI GCAGC 7 cut(s) 52, 172, 181, 184, 424, 427, 559
BciT130I CCWGG 1 cut(s) 536
BcuI ACTAGT 1 cut(s) 397
BfaI CTAG 4 cut(s) 120, 143, 398, 428
BfmI CTRYAG 1 cut(s) 66
BfoI RGCGCY 1 cut(s) 219
BfrI CTTAAG 1 cut(s) 617
BglI GCCNNNNNGGC 1 cut(s) 182
BisI GCNGC 9 cut(s) 48, 51, 66, 186, 195, 198, 438, 441, 573
BlpI GCTNAGC 1 cut(s) 219
BlsI GCNGC 9 cut(s) 49, 52, 67, 187, 196, 199, 439, 442, 574
Bme1390I CCNGG 1 cut(s) 536
Bme18I GGWCC 1 cut(s) 160
BmgT120I GGNCC 2 cut(s) 160, 383
BmiI GGNNCC 2 cut(s) 130, 175
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 2 cut(s) 28, 369
BpmI CTGGAG 1 cut(s) 236
Bpu1102I GCTNAGC 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 48, 386, 535
BsaXI ACNNNNNCTCC 2 cut(s) 234, 264
Bse1I ACTGG 3 cut(s) 177, 253, 503
Bse21I CCTNAGG 1 cut(s) 164
Bse3DI GCAATG 1 cut(s) 105
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 3 cut(s) 48, 386, 535
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 2 cut(s) 178, 210
BseNI ACTGG 3 cut(s) 177, 253, 503
BseRI GAGGAG 1 cut(s) 69
BseXI GCAGC 7 cut(s) 52, 172, 181, 184, 424, 427, 559
Bsh1236I CGCG 1 cut(s) 50
BshFI GGCC 4 cut(s) 47, 53, 333, 385
BshNI GGYRCC 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 79
BsiSI CCGG 1 cut(s) 444
BslFI GGGAC 1 cut(s) 552
BsmFI GGGAC 1 cut(s) 552
BsnI GGCC 4 cut(s) 47, 53, 333, 385
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 2 cut(s) 128, 274
Bsp1720I GCTNAGC 1 cut(s) 219
BspACI CCGC 3 cut(s) 48, 50, 575
BspANI GGCC 4 cut(s) 47, 53, 333, 385
BspCNI CTCAG 2 cut(s) 177, 211
BspFNI CGCG 1 cut(s) 50
BspLI GGNNCC 2 cut(s) 130, 175
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 2 cut(s) 123, 136
BspT107I GGYRCC 1 cut(s) 173
BspTI CTTAAG 1 cut(s) 617
BsrDI GCAATG 1 cut(s) 105
BsrI ACTGG 3 cut(s) 177, 253, 503
BssECI CCNNGG 3 cut(s) 48, 386, 535
BssMI GATC 2 cut(s) 128, 274
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 3 cut(s) 206, 246, 415
Bst6I CTCTTC 1 cut(s) 118
BstAFI CTTAAG 1 cut(s) 617
BstAPI GCANNNNNTGC 1 cut(s) 74
BstBAI YACGTR 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 504
BstDEI CTNAG 3 cut(s) 164, 219, 542
BstDSI CCRYGG 1 cut(s) 48
BstFNI CGCG 1 cut(s) 50
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 2 cut(s) 131, 277
BstMBI GATC 2 cut(s) 128, 274
BstMWI GCNNNNNNNGC 9 cut(s) 47, 74, 95, 182, 191, 194, 446, 455, 557
BstNI CCWGG 1 cut(s) 536
BstSCI CCNGG 1 cut(s) 534
BstSFI CTRYAG 1 cut(s) 66
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 7 cut(s) 52, 172, 181, 184, 424, 427, 559
BstX2I RGATCY 1 cut(s) 128
BstYI RGATCY 1 cut(s) 128
Bsu36I CCTNAGG 1 cut(s) 164
BsuRI GGCC 4 cut(s) 47, 53, 333, 385
BtgI CCRYGG 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 504
CaiI CAGNNNCTG 1 cut(s) 35
CfoI GCGC 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 160, 383
Cfr42I CCGCGG 1 cut(s) 51
Csp6I GTAC 1 cut(s) 466
CviAII CATG 3 cut(s) 260, 340, 586
CviQI GTAC 1 cut(s) 466
DdeI CTNAG 3 cut(s) 164, 219, 542
DpnI GATC 2 cut(s) 130, 276
DpnII GATC 2 cut(s) 128, 274
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 118
EarI CTCTTC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 160
Eco47III AGCGCT 1 cut(s) 217
Eco72I CACGTG 1 cut(s) 374
Eco81I CCTNAGG 1 cut(s) 164
EcoRI GAATTC 1 cut(s) 601
EcoRII CCWGG 1 cut(s) 534
EcoT22I ATGCAT 1 cut(s) 362
FaeI CATG 3 cut(s) 263, 343, 589
FaqI GGGAC 1 cut(s) 552
FatI CATG 3 cut(s) 259, 339, 585
Fnu4HI GCNGC 9 cut(s) 48, 51, 66, 186, 195, 198, 438, 441, 573
Fsp4HI GCNGC 9 cut(s) 48, 51, 66, 186, 195, 198, 438, 441, 573
FspBI CTAG 4 cut(s) 120, 143, 398, 428
GlaI GCGC 1 cut(s) 217
GluI GCNGC 9 cut(s) 48, 51, 66, 186, 195, 198, 438, 441, 573
GsuI CTGGAG 1 cut(s) 236
HaeII RGCGCY 1 cut(s) 219
HaeIII GGCC 4 cut(s) 47, 53, 333, 385
HapII CCGG 1 cut(s) 444
HhaI GCGC 1 cut(s) 218
Hin1II CATG 3 cut(s) 263, 343, 589
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HinfI GANTC 3 cut(s) 5, 226, 491
HpaII CCGG 1 cut(s) 444
HphI GGTGA 1 cut(s) 427
Hpy188III TCNNGA 1 cut(s) 280
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 3 cut(s) 65, 244, 355
HpyCH4III ACNGT 3 cut(s) 206, 246, 415
HpyCH4IV ACGT 2 cut(s) 290, 373
HpyCH4V TGCA 6 cut(s) 41, 68, 305, 360, 377, 517
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 74, 95, 182, 191, 194, 446, 455, 557
HpyF3I CTNAG 3 cut(s) 164, 219, 542
HpySE526I ACGT 2 cut(s) 290, 373
Hsp92II CATG 3 cut(s) 263, 343, 589
HspAI GCGC 1 cut(s) 216
KspI CCGCGG 1 cut(s) 51
Kzo9I GATC 2 cut(s) 128, 274
LmnI GCTCC 2 cut(s) 82, 446
LpnPI CCDG 8 cut(s) 151, 190, 266, 457, 484, 521, 548, 578
Lsp1109I GCAGC 7 cut(s) 52, 172, 181, 184, 424, 427, 559
LweI GCATC 2 cut(s) 28, 369
MaeI CTAG 4 cut(s) 120, 143, 398, 428
MaeII ACGT 2 cut(s) 290, 373
MaeIII GTNAC 2 cut(s) 31, 407
MalI GATC 2 cut(s) 130, 276
MboI GATC 2 cut(s) 128, 274
MboII GAAGA 4 cut(s) 135, 221, 229, 444
MflI RGATCY 1 cut(s) 128
MhlI GDGCHC 1 cut(s) 79
MluCI AATT 2 cut(s) 484, 601
MlyI GAGTC 1 cut(s) 485
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 6 cut(s) 90, 119, 173, 260, 344, 381
Mph1103I ATGCAT 1 cut(s) 362
MseI TTAA 2 cut(s) 212, 618
MslI CAYNNNNRTG 1 cut(s) 416
MspA1I CMGCKG 1 cut(s) 50
MspCI CTTAAG 1 cut(s) 617
MspI CCGG 1 cut(s) 444
MspR9I CCNGG 1 cut(s) 536
MvaI CCWGG 1 cut(s) 536
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 9 cut(s) 47, 74, 95, 182, 191, 194, 446, 455, 557
NdeII GATC 2 cut(s) 128, 274
NlaIII CATG 3 cut(s) 263, 343, 589
NlaIV GGNNCC 2 cut(s) 130, 175
NmeAIII GCCGAG 1 cut(s) 411
NmuCI GTSAC 1 cut(s) 407
NsiI ATGCAT 1 cut(s) 362
PfeI GAWTC 1 cut(s) 226
PflFI GACNNNGTC 1 cut(s) 158
PkrI GCNGC 9 cut(s) 49, 52, 67, 187, 196, 199, 439, 442, 574
PleI GAGTC 1 cut(s) 485
PmaCI CACGTG 1 cut(s) 374
PmlI CACGTG 1 cut(s) 374
PpsI GAGTC 1 cut(s) 485
Ppu21I YACGTR 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 534
PspCI CACGTG 1 cut(s) 374
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 2 cut(s) 130, 175
PspPI GGNCC 2 cut(s) 160, 383
PstI CTGCAG 1 cut(s) 70
PstNI CAGNNNCTG 1 cut(s) 35
PsuI RGATCY 1 cut(s) 128
PsyI GACNNNGTC 1 cut(s) 158
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 1 cut(s) 416
SacII CCGCGG 1 cut(s) 51
SaqAI TTAA 2 cut(s) 212, 618
SatI GCNGC 9 cut(s) 48, 51, 66, 186, 195, 198, 438, 441, 573
Sau3AI GATC 2 cut(s) 128, 274
Sau96I GGNCC 2 cut(s) 160, 383
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 1 cut(s) 536
SduI GDGCHC 1 cut(s) 79
SetI ASST 6 cut(s) 61, 76, 293, 376, 439, 451
SfaNI GCATC 2 cut(s) 28, 369
SfcI CTRYAG 1 cut(s) 66
Sfr303I CCGCGG 1 cut(s) 51
SgrBI CCGCGG 1 cut(s) 51
SinI GGWCC 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
SpeI ACTAGT 1 cut(s) 397
Sse9I AATT 2 cut(s) 484, 601
SsiI CCGC 3 cut(s) 48, 50, 575
SspMI CTAG 4 cut(s) 120, 143, 398, 428
StyD4I CCNGG 1 cut(s) 534
TaaI ACNGT 3 cut(s) 206, 246, 415
TaiI ACGT 2 cut(s) 293, 376
TaqI TCGA 3 cut(s) 8, 367, 494
TasI AATT 2 cut(s) 484, 601
TatI WGTACW 1 cut(s) 465
TauI GCSGC 2 cut(s) 50, 53
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 2 cut(s) 212, 618
Tru9I TTAA 2 cut(s) 212, 618
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 7 cut(s) 65, 185, 194, 197, 437, 440, 572
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 3 cut(s) 116, 552, 574
TspGWI ACGGA 1 cut(s) 495
Tth111I GACNNNGTC 1 cut(s) 158
Vha464I CTTAAG 1 cut(s) 617
VpaK11BI GGWCC 1 cut(s) 160
XapI RAATTY 2 cut(s) 484, 601
XcmI CCANNNNNNNNNTGG 1 cut(s) 542
XspI CTAG 4 cut(s) 120, 143, 398, 428
Zsp2I ATGCAT 1 cut(s) 362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.