Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006813

Physical Location & Seq

Genomic Coordinates Forward (+)

21884185 .. 21897809

Gene Structure 13625 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1165400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2646 bp

ATGTCCGCTTCTTTAGCTGCTTTTGAGCGCCCCCTACGGCCTGCTGCCTCTAACACGATGATCCAGCAGGTCTTCAAGAGTGGTCCACTTTTCATTTCTTCTAAAGGAATAGGCTGGAAGTCCTGGAAGAAGCGGTGGTTTATCCTCACACGGACTTCTTTGGTTTTCTTTAAAAATGATCCTAGCGCACTTCCACAAAGAGGCGGTGAAGTCAATTTGACTTTGGGAGGCATTGACTTGAACAATTCTGGGAGTGTTGTTGTTAGAGAAGATAAAAAGCTTCTGACAGTTTTATTTCCTGATGGACGTGATGGGCGGGCCTTCACTCTTAAGGCTGAGACGTCGGAAGACTTGTATGAGTGGAAGACAGCCCTTGAACATGCCCTTGCACAAGCTCCAAGTGCTGCCCTTGTAATGGGACACAATGGGATTTTCCGTAATGACACGAATGATACACTTGAAGGGTCCTTCCATCAATGGAGAGATAAAAGGCCTGTTAAATCTTTGGTTGTTGGAAGACCTATTCTGCTTGCGCTAGAAGATATTGATGGAGGTCCTTCCTTCCTGGAGAAAGCTCTTCGGTTCCTGGAAAAGTTTGGTACTAAAGTTGAAGGAATATTGAGACAGTCTGCGGATGTTGAGGAGGTAGAACGTAGAGTACAAGAATATGAGCAAGGCAAGACTGAATTTAATCCGGATGAGGATGCTCATGTTGTTGGCGATTGTGTCAAGCATGTTCTGAGAGAGCTGCCCTCATCTCCAGTACCAGCTTCTTGCTGCACAGCATTGCTAGAGGCTTATAAAATTGATCGAAAGGAAGCTCGGGTTAGTGCAATGCGCTCTTCAATATTGGAGACATTTCCCGAGCCAAATCGCCGTTTATTACAGAGAATTTTGAAGATGATGCATGTCATATCTTCTCACTCTGATGAGAATCGGATGACTCCATCTGCAGTTGCTGCATGCATGGCACCCTTGCTCTTACGCCCTCTATTAGCTGGTGAATGTGAGCTGGATGATGACTTTGATCTTAATGGTGATAATTCTGCCCAGCTTATTGCTGCTGCAAATGCTGCAAATAATGCTCAAGCAATCATCACAACTCTTTTGGAGGAGTATGAAAACGTTTTTGATGATGAAAATCTTCTAAGGTGTTCGATCTCTGCTGATTCTCGAATTGAGAATAGTGGAAGTGAAGATTCATCTGATGATGAAAATCTTGATCTGAAAGACAACGGTTACCATGATGCAGAGAATGAAGTTGATCCGGAAACAGATGATGACCCGGATCGAGTTCATAGTGGAAAGTTGAGTGAAAGCAGTGGTTATGCTGGCAGTGACCTCTATGATTATAAGGCATTTGGGGTTGATGATTCAGATGTTGGATCACCTAAAGGAAATCATGCGTCAGCTGAGAGTTCAAGTTCACATGTTCCTCAAACCATCAGAGATCCAAATGTTGAAGTTGTGGAAAGAGGAAGCAAACCAATTACAGAAAATGAAAATTTAATTAGTGAGACAGAAACACCAAGTGCATCACCTGCCGGAGATTCTTACAGATCAATGGGAGAGATTCTGTCAGCAATGGATCCAGGGCATCCTTTACCAGTGTCTGGACTTGAATCAGGAACTGTGAAGAAAGTGGCCAAAGTTACAGGCTCCAATCATAACGCAAAGCGATCGGCATTTTGGGGAAGAAACAATGCAAGAAAGACACCGTCAATGGAATCAGTCGATTCTTCTGGAGAGGAAGAGCTTTCAATTCAGAGGCTTGAGATTGCGAAAAACGACTTGCAGCAGAGGATTGCGAAAGAGGCTAGAGGAAATGCAATTTTACAAGCTAGTTTGGAGAGAAGGAAGCAAGCTTTACATGAGCGGCGCTTGGCACTGGAACAAGATGTTTCAAGATTGCAAGAGCAGCTACAAGCTGAGCGAGATCTTAGAGCGGCATTAGAGGTCGGTTTGAGCATGTCTTCTGGACAGTTGGCCAGTTCATGTGGCATGGATTCTAAGACACGGGCTGAGCTTGAGGAGATAGCCCTTGCTGAAGCGGATGTGGCCAGGTTGAAGCAGAAAGTTGCAGAACTTCACCATCAACTTAATCAACAGCGACAGCATCATTATGGTTCTCTCTCTGATGCTTGTGACCGTTATCAAAATGGCCAAAATAATAATTCCCAACAGAGGTTTCTGCAGCAAGATTTTGATGCAACCCTTGCCTTCTGTAATCATGAAAGGAAACAAAGGAATGAGGAGAGTTTGCTGGGGGCAGATTGGAGGACTATCAAAGGCCAAATTTTAGCATCTGCCAGCAGTACAAGGCAGCCTTCTAGGAAGCAATTCATGGATCCGGCTAGCAGAAGTGAATCAAAAAATACTGATGCATCAACAAGTTTGTCTGTTGATGAGCTTTCTGCTGTGGATTCTGCTTCCATGCCATCGACTTCAAGAGCACCAGAGGCGGTGGATTATCCAAGGCATCCATCAACAGCATCTTCTGCCTTGGTAGAACTACCAACTCGCCTTGATTTCTTCAAGGAAAGACGTTCCCAGCTGATGGAACAGCTTCATAATCTTGATTTAAATTATGGCACGGCATCTTTGCAAGACTCAGTTTATAGACCATCATCTCCACCATGGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0000919 GO:0003674 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006996 GO:0007049 GO:0008047 GO:0008150 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009832 GO:0009920 GO:0009987 GO:0016020 GO:0016043 GO:0022402 GO:0022607 GO:0030234 GO:0030695 GO:0031976 GO:0031984 GO:0032506 GO:0034357 GO:0042546 GO:0042651 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043547 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0050790 GO:0051301 GO:0051336 GO:0051345 GO:0055035 GO:0060589 GO:0061640 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0098772 GO:0140014 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lzipper-MIP1 PH RhoGAP

Protein Analysis

882

Amino Acids

97.33

Weight (kDa)

5.18

Isoelectric Point (pI)

53.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH	PF00169	25 - 129	1.1e-12	PH domain
RhoGAP	PF00620	186 - 330	7.9e-34	RhoGAP domain
Lzipper-MIP1	PF14389	623 - 703	1.2e-21	Leucine-zipper of ternary complex factor MIP1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006813)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19390 AT5G19390 AT5G19390 AT5G19390
fragaria_vesca	FvH4_3g27830 FvH4_3g27830 FvH4_3g27830 FvH4_3g27830
malus_domestica	MD03G1165400.v1.1 MD11G1184700.v1.1
prunus_persica	Prupe.4G265200_v2.0.a1 Prupe.4G265200_v2.0.a1 Prupe.4G265200_v2.0.a1 Prupe.4G265300_v2.0.a1
pyrus_communis	pycom03g11990 pycom12g08810
rosa_chinensis	RchiOBHm_Chr5g0050541
rosa_laevigata	RLG00000034705
rosa_multiflora	Rmu_sc0004301.1_g000009
rosa_roxburghii	Rroxscaffold_1G00030350
rosa_rugosa	Rorug05G0261400
rosa_samantha	Rh5AG333300 Rh5BG343200 Rh5CG370800 Rh5DG355900
rosa_wichuraiana	Rw5G031480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	801, 1353
AarI	CACCTGC	1 cut(s)	1549
AatII	GACGTC	1 cut(s)	344
Acc36I	ACCTGC	2 cut(s)	58, 1549
AccB1I	GGYRCC	1 cut(s)	970
AccB7I	CCANNNNNTGG	2 cut(s)	1613, 2557
AccBSI	CCGCTC	2 cut(s)	1876, 1946
AccIII	TCCGGA	2 cut(s)	694, 1267
AciI	CCGC	9 cut(s)	6, 133, 204, 316, 632, 1876, 1946, 2051, 2462
AclI	AACGTT	1 cut(s)	1125
AcoI	YGGCCR	4 cut(s)	1644, 1986, 2058, 2161
AcsI	RAATTY	4 cut(s)	686, 891, 1504, 2295
AcuI	CTGAAG	1 cut(s)	2067
AcyI	GRCGYC	1 cut(s)	341
AdeI	CACNNNGTG	1 cut(s)	1532
AfaI	GTAC	4 cut(s)	601, 660, 765, 2317
AfiI	CCNNNNNNNGG	5 cut(s)	200, 415, 1613, 2184, 2557
AflII	CTTAAG	1 cut(s)	329
AflIII	ACRYGT	1 cut(s)	1429
AjiI	CACGTC	1 cut(s)	308
AjnI	CCWGG	5 cut(s)	122, 564, 585, 1591, 2060
AjuI	GAANNNNNNNTTGG	2 cut(s)	2172, 2204
Alw21I	GWGCWC	1 cut(s)	2455
Alw26I	GTCTC	4 cut(s)	332, 616, 848, 1511
AlwNI	CAGNNNCTG	3 cut(s)	959, 1613, 1631
Ama87I	CYCGRG	2 cut(s)	822, 863
Aor13HI	TCCGGA	2 cut(s)	694, 1267
AoxI	GGCC	8 cut(s)	38, 318, 491, 1644, 1986, 2058, 2161, 2290
ApoI	RAATTY	4 cut(s)	686, 891, 1504, 2295
Asp700I	GAANNNNTTC	3 cut(s)	1143, 2339, 2565
AspLEI	GCGC	5 cut(s)	30, 188, 535, 840, 1881
AspS9I	GGNCC	4 cut(s)	83, 318, 465, 554
AsuC2I	CCSGG	1 cut(s)	1286
AsuHPI	GGTGA	6 cut(s)	218, 1013, 1049, 1380, 1530, 2081
AsuNHI	GCTAGC	1 cut(s)	2354
AvaI	CYCGRG	2 cut(s)	822, 863
AvaII	GGWCC	3 cut(s)	83, 465, 554
BaeI	ACNNNNGTAYC	2 cut(s)	591, 624
BalI	TGGCCA	4 cut(s)	1646, 1988, 2060, 2163
BamHI	GGATCC	2 cut(s)	1588, 2347
BanI	GGYRCC	1 cut(s)	970
BbsI	GAAGAC	5 cut(s)	64, 354, 371, 523, 1965
Bbv12I	GWGCWC	1 cut(s)	2455
BceAI	ACGGC	3 cut(s)	53, 861, 2610
BcgI	CGANNNNNNTGC	2 cut(s)	1662, 1696
BciT130I	CCWGG	5 cut(s)	124, 566, 587, 1593, 2062
BcnI	CCSGG	1 cut(s)	1286
BcoDI	GTCTC	4 cut(s)	332, 616, 848, 1511
BfaI	CTAG	7 cut(s)	183, 536, 791, 1818, 1842, 2331, 2355
BfmI	CTRYAG	2 cut(s)	951, 2192
BfoI	RGCGCY	2 cut(s)	31, 1882
BfrI	CTTAAG	1 cut(s)	329
BfuAI	ACCTGC	2 cut(s)	58, 1549
BglII	AGATCT	1 cut(s)	1936
BlpI	GCTNAGC	2 cut(s)	1929, 2022
Bme1390I	CCNGG	6 cut(s)	124, 566, 587, 1286, 1593, 2062
Bme18I	GGWCC	3 cut(s)	83, 465, 554
BmeT110I	CYCGRG	2 cut(s)	822, 863
BmgBI	CACGTC	1 cut(s)	308
BmgT120I	GGNCC	4 cut(s)	83, 318, 465, 554
BmiI	GGNNCC	6 cut(s)	466, 584, 972, 1590, 1660, 2349
BmrFI	CCNGG	6 cut(s)	124, 566, 587, 1286, 1593, 2062
BmtI	GCTAGC	1 cut(s)	2358
BpiI	GAAGAC	5 cut(s)	64, 354, 371, 523, 1965
BplI	GAGNNNNNCTC	2 cut(s)	737, 769
BpmI	CTGGAG	3 cut(s)	587, 744, 1764
Bpu1102I	GCTNAGC	2 cut(s)	1929, 2022
BpuEI	CTTGAG	3 cut(s)	1071, 1793, 2048
BpuMI	CCSGG	1 cut(s)	1286
BsaBI	GATNNNNATC	3 cut(s)	933, 1140, 1215
BsaHI	GRCGYC	1 cut(s)	341
BsaJI	CCNNGG	4 cut(s)	1592, 2474, 2502, 2636
BsaWI	WCCGGW	2 cut(s)	694, 1267
Bsc4I	CCNNNNNNNGG	5 cut(s)	200, 415, 1613, 2184, 2557
Bse1I	ACTGG	4 cut(s)	761, 1607, 1893, 1989
Bse3DI	GCAATG	3 cut(s)	785, 840, 1590
Bse8I	GATNNNNATC	3 cut(s)	933, 1140, 1215
BseAI	TCCGGA	2 cut(s)	694, 1267
BseBI	CCWGG	5 cut(s)	124, 566, 587, 1593, 2062
BseDI	CCNNGG	4 cut(s)	1592, 2474, 2502, 2636
BseGI	GGATG	8 cut(s)	640, 703, 709, 945, 1021, 1597, 2059, 2479
BseJI	GATNNNNATC	3 cut(s)	933, 1140, 1215
BseLI	CCNNNNNNNGG	5 cut(s)	200, 415, 1613, 2184, 2557
BseMI	GCAATG	3 cut(s)	785, 840, 1590
BseMII	CTCAG	6 cut(s)	327, 731, 1404, 1920, 2013, 2625
BseNI	ACTGG	4 cut(s)	761, 1607, 1893, 1989
BseRI	GAGGAG	4 cut(s)	656, 1127, 2045, 2267
BseYI	CCCAGC	3 cut(s)	1050, 2263, 2550
BsgI	GTGCAG	1 cut(s)	763
Bsh1285I	CGRYCG	1 cut(s)	1682
BshFI	GGCC	8 cut(s)	40, 320, 493, 1646, 1988, 2060, 2163, 2292
BshNI	GGYRCC	1 cut(s)	970
BsiEI	CGRYCG	1 cut(s)	1682
BsiHKAI	GWGCWC	1 cut(s)	2455
BsiHKCI	CYCGRG	2 cut(s)	822, 863
BsiSI	CCGG	5 cut(s)	695, 1268, 1286, 1545, 2351
BslFI	GGGAC	1 cut(s)	432
BslI	CCNNNNNNNGG	5 cut(s)	200, 415, 1613, 2184, 2557
BsmAI	GTCTC	4 cut(s)	332, 616, 848, 1511
BsmBI	CGTCTC	1 cut(s)	332
BsmFI	GGGAC	1 cut(s)	432
BsnI	GGCC	8 cut(s)	40, 320, 493, 1646, 1988, 2060, 2163, 2292
BsoBI	CYCGRG	2 cut(s)	822, 863
Bsp1286I	GDGCHC	1 cut(s)	2455
Bsp13I	TCCGGA	2 cut(s)	694, 1267
Bsp1720I	GCTNAGC	2 cut(s)	1929, 2022
Bsp19I	CCATGG	1 cut(s)	2636
BspACI	CCGC	9 cut(s)	6, 133, 204, 316, 632, 1876, 1946, 2051, 2462
BspANI	GGCC	8 cut(s)	40, 320, 493, 1646, 1988, 2060, 2163, 2292
BspCNI	CTCAG	6 cut(s)	328, 732, 1405, 1921, 2014, 2624
BspEI	TCCGGA	2 cut(s)	694, 1267
BspHI	TCATGA	1 cut(s)	2230
BspLI	GGNNCC	6 cut(s)	466, 584, 972, 1590, 1660, 2349
BspMAI	CTGCAG	2 cut(s)	955, 2196
BspMI	ACCTGC	2 cut(s)	58, 1549
BspOI	GCTAGC	1 cut(s)	2358
BspQI	GCTCTTC	3 cut(s)	582, 847, 1746
BspT107I	GGYRCC	1 cut(s)	970
BspTI	CTTAAG	1 cut(s)	329
BsrBI	CCGCTC	2 cut(s)	1876, 1946
BsrDI	GCAATG	3 cut(s)	785, 840, 1590
BsrI	ACTGG	4 cut(s)	761, 1607, 1893, 1989
BssECI	CCNNGG	4 cut(s)	1592, 2474, 2502, 2636
BssNI	GRCGYC	1 cut(s)	341
BssT1I	CCWWGG	3 cut(s)	2474, 2502, 2636
Bst2UI	CCWGG	5 cut(s)	124, 566, 587, 1593, 2062
Bst4CI	ACNGT	7 cut(s)	289, 627, 1238, 1633, 1719, 1983, 2150
Bst6I	CTCTTC	3 cut(s)	582, 847, 1746
BstACI	GRCGYC	1 cut(s)	341
BstAFI	CTTAAG	1 cut(s)	329
BstAPI	GCANNNNNTGC	6 cut(s)	959, 1073, 1082, 1541, 2216, 2498
BstC8I	GCNNGC	8 cut(s)	42, 318, 531, 964, 1333, 1863, 2311, 2356
BstDEI	CTNAG	9 cut(s)	336, 740, 1148, 1413, 1929, 1940, 2010, 2022, 2611
BstDSI	CCRYGG	1 cut(s)	2636
BstEII	GGTNACC	1 cut(s)	1238
BstF5I	GGATG	8 cut(s)	640, 703, 709, 945, 1021, 1597, 2059, 2479
BstH2I	RGCGCY	2 cut(s)	31, 1882
BstHHI	GCGC	5 cut(s)	30, 188, 535, 840, 1881
BstMAI	GTCTC	4 cut(s)	332, 616, 848, 1511
BstMCI	CGRYCG	1 cut(s)	1682
BstNI	CCWGG	5 cut(s)	124, 566, 587, 1593, 2062
BstNSI	RCATGY	6 cut(s)	383, 737, 911, 966, 1433, 1972
BstPI	GGTNACC	1 cut(s)	1238
BstSCI	CCNGG	6 cut(s)	122, 564, 585, 1284, 1591, 2060
BstSFI	CTRYAG	2 cut(s)	951, 2192
BstV2I	GAAGAC	5 cut(s)	64, 354, 371, 523, 1965
BstX2I	RGATCY	4 cut(s)	1450, 1588, 1936, 2347
BstYI	RGATCY	4 cut(s)	1450, 1588, 1936, 2347
BsuRI	GGCC	8 cut(s)	40, 320, 493, 1646, 1988, 2060, 2163, 2292
BtgI	CCRYGG	1 cut(s)	2636
BtrI	CACGTC	1 cut(s)	308
BtsCI	GGATG	8 cut(s)	640, 703, 709, 945, 1021, 1597, 2059, 2479
BtsI	GCAGTG	2 cut(s)	1327, 1342
BtsIMutI	CAGTG	4 cut(s)	1327, 1342, 1614, 1886
BveI	ACCTGC	2 cut(s)	58, 1549
Cac8I	GCNNGC	8 cut(s)	42, 318, 531, 964, 1333, 1863, 2311, 2356
CaiI	CAGNNNCTG	3 cut(s)	959, 1613, 1631
CciI	TCATGA	1 cut(s)	2230
CfoI	GCGC	5 cut(s)	30, 188, 535, 840, 1881
Cfr13I	GGNCC	4 cut(s)	83, 318, 465, 554
CseI	GACGC	1 cut(s)	1395
Csp6I	GTAC	4 cut(s)	600, 659, 764, 2316
CviQI	GTAC	4 cut(s)	600, 659, 764, 2316
DdeI	CTNAG	9 cut(s)	336, 740, 1148, 1413, 1929, 1940, 2010, 2022, 2611
DraI	TTTAAA	2 cut(s)	172, 2583
DraIII	CACNNNGTG	1 cut(s)	1532
EaeI	YGGCCR	4 cut(s)	1644, 1986, 2058, 2161
Eam1104I	CTCTTC	3 cut(s)	582, 847, 1746
EarI	CTCTTC	3 cut(s)	582, 847, 1746
Eco130I	CCWWGG	3 cut(s)	2474, 2502, 2636
Eco147I	AGGCCT	1 cut(s)	493
Eco47I	GGWCC	3 cut(s)	83, 465, 554
Eco57I	CTGAAG	1 cut(s)	2067
Eco88I	CYCGRG	2 cut(s)	822, 863
Eco91I	GGTNACC	1 cut(s)	1238
EcoO109I	RGGNCCY	2 cut(s)	465, 554
EcoO65I	GGTNACC	1 cut(s)	1238
EcoRII	CCWGG	5 cut(s)	122, 564, 585, 1591, 2060
EcoT14I	CCWWGG	3 cut(s)	2474, 2502, 2636
EcoT22I	ATGCAT	3 cut(s)	909, 968, 2386
ErhI	CCWWGG	3 cut(s)	2474, 2502, 2636
Esp3I	CGTCTC	1 cut(s)	332
FalI	AAGNNNNNCTT	2 cut(s)	2311, 2343
FaqI	GGGAC	1 cut(s)	432
FauI	CCCGC	1 cut(s)	309
FokI	GGATG	8 cut(s)	647, 710, 716, 952, 1028, 1584, 2066, 2466
FspBI	CTAG	7 cut(s)	183, 536, 791, 1818, 1842, 2331, 2355
GlaI	GCGC	5 cut(s)	29, 187, 534, 839, 1880
GsaI	CCCAGC	3 cut(s)	1054, 2267, 2554
GsuI	CTGGAG	3 cut(s)	587, 744, 1764
HaeII	RGCGCY	2 cut(s)	31, 1882
HaeIII	GGCC	8 cut(s)	40, 320, 493, 1646, 1988, 2060, 2163, 2292
HapII	CCGG	5 cut(s)	695, 1268, 1286, 1545, 2351
HgaI	GACGC	1 cut(s)	1395
HhaI	GCGC	5 cut(s)	30, 188, 535, 840, 1881
Hin1I	GRCGYC	1 cut(s)	341
Hin6I	GCGC	5 cut(s)	28, 186, 533, 838, 1879
HinP1I	GCGC	5 cut(s)	28, 186, 533, 838, 1879
HindIII	AAGCTT	2 cut(s)	278, 1863
HpaII	CCGG	5 cut(s)	695, 1268, 1286, 1545, 2351
HphI	GGTGA	6 cut(s)	218, 1013, 1049, 1380, 1530, 2081
Hpy166II	GTNNAC	2 cut(s)	86, 1427
Hpy8I	GTNNAC	2 cut(s)	86, 1427
Hpy99I	CGWCG	1 cut(s)	346
HpyAV	CCTTC	9 cut(s)	331, 455, 478, 567, 571, 605, 1848, 2230, 2337
HpyCH4III	ACNGT	7 cut(s)	289, 627, 1238, 1633, 1719, 1983, 2150
HpyCH4IV	ACGT	5 cut(s)	307, 341, 652, 1125, 2545
HpyF3I	CTNAG	9 cut(s)	336, 740, 1148, 1413, 1929, 1940, 2010, 2022, 2611
HpySE526I	ACGT	5 cut(s)	307, 341, 652, 1125, 2545
Hsp92I	GRCGYC	1 cut(s)	341
HspAI	GCGC	5 cut(s)	28, 186, 533, 838, 1879
Kpn2I	TCCGGA	2 cut(s)	694, 1267
LguI	GCTCTTC	3 cut(s)	582, 847, 1746
LmnI	GCTCC	2 cut(s)	400, 1664
MaeI	CTAG	7 cut(s)	183, 536, 791, 1818, 1842, 2331, 2355
MaeII	ACGT	5 cut(s)	307, 341, 652, 1125, 2545
MaeIII	GTNAC	4 cut(s)	1238, 1337, 1651, 2144
MbiI	CCGCTC	2 cut(s)	1876, 1946
MflI	RGATCY	4 cut(s)	1450, 1588, 1936, 2347
MhlI	GDGCHC	1 cut(s)	2455
MlsI	TGGCCA	4 cut(s)	1646, 1988, 2060, 2163
MluNI	TGGCCA	4 cut(s)	1646, 1988, 2060, 2163
MlyI	GAGTC	2 cut(s)	937, 2603
MmeI	TCCRAC	3 cut(s)	324, 493, 1363
Mox20I	TGGCCA	4 cut(s)	1646, 1988, 2060, 2163
Mph1103I	ATGCAT	3 cut(s)	909, 968, 2386
MroI	TCCGGA	2 cut(s)	694, 1267
MroXI	GAANNNNTTC	3 cut(s)	1143, 2339, 2565
MscI	TGGCCA	4 cut(s)	1646, 1988, 2060, 2163
MseI	TTAA	8 cut(s)	171, 330, 498, 690, 1032, 1508, 2100, 2582
MslI	CAYNNNNRTG	2 cut(s)	927, 2121
Msp20I	TGGCCA	4 cut(s)	1646, 1988, 2060, 2163
MspA1I	CMGCKG	2 cut(s)	1412, 2554
MspCI	CTTAAG	1 cut(s)	329
MspI	CCGG	5 cut(s)	695, 1268, 1286, 1545, 2351
MspR9I	CCNGG	6 cut(s)	124, 566, 587, 1286, 1593, 2062
MvaI	CCWGG	5 cut(s)	124, 566, 587, 1593, 2062
NciI	CCSGG	1 cut(s)	1286
NcoI	CCATGG	1 cut(s)	2636
NheI	GCTAGC	1 cut(s)	2354
NlaIV	GGNNCC	6 cut(s)	466, 584, 972, 1590, 1660, 2349
NmuCI	GTSAC	2 cut(s)	1337, 2144
NsiI	ATGCAT	3 cut(s)	909, 968, 2386
NspI	RCATGY	6 cut(s)	383, 737, 911, 966, 1433, 1972
PaeI	GCATGC	1 cut(s)	966
PagI	TCATGA	1 cut(s)	2230
PaqCI	CACCTGC	1 cut(s)	1549
PceI	AGGCCT	1 cut(s)	493
PciI	ACATGT	1 cut(s)	1429
PciSI	GCTCTTC	3 cut(s)	582, 847, 1746
PdmI	GAANNNNTTC	3 cut(s)	1143, 2339, 2565
PflFI	GACNNNGTC	1 cut(s)	1717
PflMI	CCANNNNNTGG	2 cut(s)	1613, 2557
PfoI	TCCNGGA	3 cut(s)	122, 564, 585
Ple19I	CGATCG	1 cut(s)	1682
PleI	GAGTC	2 cut(s)	937, 2603
PpsI	GAGTC	2 cut(s)	937, 2603
PpuMI	RGGWCCY	2 cut(s)	465, 554
PscI	ACATGT	1 cut(s)	1429
PsiI	TTATAA	2 cut(s)	801, 1353
Psp1406I	AACGTT	1 cut(s)	1125
Psp5II	RGGWCCY	2 cut(s)	465, 554
Psp6I	CCWGG	5 cut(s)	122, 564, 585, 1591, 2060
PspEI	GGTNACC	1 cut(s)	1238
PspFI	CCCAGC	3 cut(s)	1050, 2263, 2550
PspGI	CCWGG	5 cut(s)	122, 564, 585, 1591, 2060
PspN4I	GGNNCC	6 cut(s)	466, 584, 972, 1590, 1660, 2349
PspPI	GGNCC	4 cut(s)	83, 318, 465, 554
PspPPI	RGGWCCY	2 cut(s)	465, 554
PsrI	GAACNNNNNNTAC	2 cut(s)	642, 674
PstI	CTGCAG	2 cut(s)	955, 2196
PstNI	CAGNNNCTG	3 cut(s)	959, 1613, 1631
PsuI	RGATCY	4 cut(s)	1450, 1588, 1936, 2347
PsyI	GACNNNGTC	1 cut(s)	1717
PvuI	CGATCG	1 cut(s)	1682
PvuII	CAGCTG	2 cut(s)	1412, 2554
RsaI	GTAC	4 cut(s)	601, 660, 765, 2317
RsaNI	GTAC	4 cut(s)	600, 659, 764, 2316
RseI	CAYNNNNRTG	2 cut(s)	927, 2121
SapI	GCTCTTC	3 cut(s)	582, 847, 1746
SaqAI	TTAA	8 cut(s)	171, 330, 498, 690, 1032, 1508, 2100, 2582
Sau96I	GGNCC	4 cut(s)	83, 318, 465, 554
SchI	GAGTC	2 cut(s)	937, 2603
ScrFI	CCNGG	6 cut(s)	124, 566, 587, 1286, 1593, 2062
SduI	GDGCHC	1 cut(s)	2455
SfcI	CTRYAG	2 cut(s)	951, 2192
SinI	GGWCC	3 cut(s)	83, 465, 554
SmiI	ATTTAAAT	1 cut(s)	2583
SmiMI	CAYNNNNRTG	2 cut(s)	927, 2121
SmlI	CTYRAG	4 cut(s)	329, 1086, 1772, 2027
SmoI	CTYRAG	4 cut(s)	329, 1086, 1772, 2027
SphI	GCATGC	1 cut(s)	966
SseBI	AGGCCT	1 cut(s)	493
SsiI	CCGC	9 cut(s)	6, 133, 204, 316, 632, 1876, 1946, 2051, 2462
SspI	AATATT	2 cut(s)	618, 849
SspMI	CTAG	7 cut(s)	183, 536, 791, 1818, 1842, 2331, 2355
StuI	AGGCCT	1 cut(s)	493
StyD4I	CCNGG	6 cut(s)	122, 564, 585, 1284, 1591, 2060
StyI	CCWWGG	3 cut(s)	2474, 2502, 2636
SwaI	ATTTAAAT	1 cut(s)	2583
TaaI	ACNGT	7 cut(s)	289, 627, 1238, 1633, 1719, 1983, 2150
TaiI	ACGT	5 cut(s)	310, 344, 655, 1128, 2548
TaqI	TCGA	6 cut(s)	811, 1157, 1174, 1291, 1734, 2441
TatI	WGTACW	2 cut(s)	658, 2315
TauI	GCSGC	2 cut(s)	1879, 1949
Tru1I	TTAA	8 cut(s)	171, 330, 498, 690, 1032, 1508, 2100, 2582
Tru9I	TTAA	8 cut(s)	171, 330, 498, 690, 1032, 1508, 2100, 2582
TscAI	CASTG	4 cut(s)	1327, 1342, 1614, 1893
TseFI	GTSAC	2 cut(s)	1337, 2144
Tsp45I	GTSAC	2 cut(s)	1337, 2144
TspGWI	ACGGA	2 cut(s)	166, 425
TspRI	CASTG	4 cut(s)	1327, 1342, 1614, 1893
Tth111I	GACNNNGTC	1 cut(s)	1717
Van91I	CCANNNNNTGG	2 cut(s)	1613, 2557
Vha464I	CTTAAG	1 cut(s)	329
VpaK11BI	GGWCC	3 cut(s)	83, 465, 554
XapI	RAATTY	4 cut(s)	686, 891, 1504, 2295
XceI	RCATGY	6 cut(s)	383, 737, 911, 966, 1433, 1972
XmnI	GAANNNNTTC	3 cut(s)	1143, 2339, 2565
XspI	CTAG	7 cut(s)	183, 536, 791, 1818, 1842, 2331, 2355
ZraI	GACGTC	1 cut(s)	342
Zsp2I	ATGCAT	3 cut(s)	909, 968, 2386

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.