Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0006813

Physical Location & Seq

Genomic Coordinates Reverse (-)

51763904 .. 51777737

Gene Structure 13834 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G031480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2634 bp

ATGTCGGCTTCTTTAGCTGCTTTTGAGCGCCCCCGCCCCGGAGCTTCTAACACGGTCTTCAAGAGTGGCCCATTATTCATATCTTCAAAAGGAATAGGGTGGAAGTCTTGGAAGAAGCGGTGGTTTATCCTCACACGGACTTCTTGGTTTTCTTTAAAAATGATCCTGTTAAGCGCACTTCCACAAAAAGGTGGTGAAGTATATTTGACTTTAGGAGGCATTGACCTGAACAATTCTGGGAGTGTTGTTGTTAGAGAAGATAAAAAGCTGCTGACTGTTTTATTTCCTGATGGACGTGATGGGCGGGCTTTCACTCTTAAGGCTGAGACATCAGAAGACTTGTACGAGTGGAAGACAGCCCTTGAACATGCCCTTGCACAAGCTCCAAGTGCTGCCCTTGTCATGGGGCACAATGGGATTTTCAGAAATGACACAAATGATACAATAGAAGGGTCTTTCCATCAATGGAGGGATAAGCGTCCTGTTAAATCTTTGGTCGTTGGAAGACCAATTCTGCTTGCCCTAGAAGATATTGATGGAGGTCCTTCTTTCCTCGAGAAAGCTCTTCGGTTCCTTGAAAAGTTTGGTACTAAAGTTGAAGGAATTTTGCGACAGTCTGCTGATGTTGAGGAGGTAGAACGCAGGGTACAAGAGTATGAACAAGGCAAGACTGAATTTGATCCTGATGAGGATGCTCATGTTGTTGGTGATTGTGTCAAGCACGTTCTGAGAGAGCTGCCCTCATCTCCAGTACCAGCTTCTTGCTGCACTGCCTTGCTGGAGGCTTATAAAATTGATCGAAAGGAAGCTCGGATCAATGCAATGCGGTCTTCAATATTAGAGACGTTTCCTGAACCAAACCGCCGTTTATTACAAAGAATTTTAAAGATGATGCATACCATCTCTTCTCACTCTCATGAGAATCGAATGAATCCTTCAGCAGTTGCTGCTTGCATGGCACCCTTGCTCTTACGCCCTCTATTGGCTGGTGAATGTGAGTTGGATGATGACTTTGATATTAATGGTGATAATTCTGCCCAGCTTCTTGCTGCTGCAAATGCTGCCAATAATGCTCAAGCAATCATCACTACGCTTTTGGAGGAGTTTGAAAACATTTTTGATGATGAGAATTTACTGCGGTGTTCGATCTCAGCTGATTCTCGAATTGAAAATAGTGGCAGTGATGATTCGTCCGACGATGACAACCTTGATATAAAAGATAATGGTTACCATGATGCCGAAAATGAAGTTGATGTGGAAACAGATGATGACCAAGATCGTGTACACAGTGGGAAATTGAGTGAAAGCAGCGGTTATGCTGGCAGTGACCTCTATGATTATAAGGCATTTGGGGCTGATGATTCTGATGTTGGATCGCCTAATGGAAATCATGGTTCAGCTGAGAGTTCAAAATCAGTTGTTCCTCAACAGAATTCAAACGTCGAAGGGATGGATAGAGGAGGCAAACAGATAAAAGAAAATGAAAATTCAATTAGTGAGAATGGTGAAACAAGCATACCAAGCACATCACCTGCGGGAGAATCGTACAGATCAATGGGAGAGATTCTATCAGCAATGGATCCAGGGCATCCTTTACCAGTTGTGTCTGAATCAGGAGCTGTTAAGGCAGTGGGTAAAGTAACAGGGTCCAATCATAGTGCAAAGCGAACAGCATTTTGGGGAAGAAGCAATGCAAGAAAGACACTATCCATGGAATCAGTCGATTCTTCTGGAGAGGAAGAGCTTTCTATTCAGAGGCTTGAGATTGCTAAAAATGACCTGCAGCACAGGATTGCAAAAGAGGCTAGAGGAAATGCAATTTTACAAGCTAGCTTGGAGAGAAGAAAGCAAGCTTTACATGAGAGGCGCCTGGCACTGGAACAAGATGTTTCAAGATTACAAGAACAGCTACAAGCTGAAAGAGATCTTAGAGCTGCATTAGAGGTTGGTTTGAGCATGTCTTCTGGACAGTTAGCCAGTTCACGTGGCATGGATTCTAAGACAAGAGCTGAACTTGAGGAGATAGCTCTTGCTGAAGCAGATGTGGCCAGGTTGAAACAGAAAGTTGCAGAACTCCACCATCAACTTAATCAGCAGCGACAGAATCATTATGGTTCTCTCTCTGATGCATGTGACCGTTACCAGAACGTCCAAAATCATAATTCTCAACAGAGGTTTCTTCAGCAAGATTTTGATGCAACCCTCGCCTTTTGTAATCATGAACGGAAACAGAGGAATGAGGAGAATTTGTTGGGGGCAGATTGGAGGAATATCAAAGGACAAATTCTAGCATCTGGAAGTAGCACAAGGCAGCCATCAAGGAAGCAATTCATGGATCCAGGTAGCTTAAGTGAATCCAAAAGTACTGATGTATCCACAAGTTTGTCTGTTGATGAACTTTCAGCTGTTGATTCTGCTTCCATTCCCTCCACTTCAAGAGCACCAGAGGTGGTGGATTATCCAAGACATCCATCAACAGCATCTTCAGCCTTGGTAGAATTAACAACTCGTCTTGATTTCTTCAAGGAAAGACGTTCTCAGCTAATGGAACAGCTTCATAACCTTGATTTAAATTATGGCGCAACATCTTTGCAAGACTCAGTCTATAGACCATCGTCCCCACCATGGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0000919 GO:0003674 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006996 GO:0007049 GO:0008047 GO:0008150 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009832 GO:0009920 GO:0009987 GO:0016020 GO:0016043 GO:0022402 GO:0022607 GO:0030234 GO:0030695 GO:0031976 GO:0031984 GO:0032506 GO:0034357 GO:0042546 GO:0042651 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043547 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0050790 GO:0051301 GO:0051336 GO:0051345 GO:0055035 GO:0060589 GO:0061640 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0098772 GO:0140014 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lzipper-MIP1 PH RhoGAP

Protein Analysis

877

Amino Acids

96.86

Weight (kDa)

5.22

Isoelectric Point (pI)

51.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH	PF00169	20 - 125	8.5e-09	PH domain
RhoGAP	PF00620	182 - 326	2.8e-34	RhoGAP domain
Lzipper-MIP1	PF14389	619 - 699	1.9e-21	Leucine-zipper of ternary complex factor MIP1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006813)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19390 AT5G19390 AT5G19390 AT5G19390
fragaria_vesca	FvH4_3g27830 FvH4_3g27830 FvH4_3g27830 FvH4_3g27830
malus_domestica	MD03G1165400.v1.1 MD11G1184700.v1.1
prunus_persica	Prupe.4G265200_v2.0.a1 Prupe.4G265200_v2.0.a1 Prupe.4G265200_v2.0.a1 Prupe.4G265300_v2.0.a1
pyrus_communis	pycom03g11990 pycom12g08810
rosa_chinensis	RchiOBHm_Chr5g0050541
rosa_laevigata	RLG00000034705
rosa_multiflora	Rmu_sc0004301.1_g000009
rosa_roxburghii	Rroxscaffold_1G00030350
rosa_rugosa	Rorug05G0261400
rosa_samantha	Rh5AG333300 Rh5BG343200 Rh5CG370800 Rh5DG355900
rosa_wichuraiana	Rw5G031480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	789, 1341
AarI	CACCTGC	1 cut(s)	1540
Acc36I	ACCTGC	2 cut(s)	1540, 1788
AccB1I	GGYRCC	2 cut(s)	958, 1866
AciI	CCGC	8 cut(s)	34, 118, 304, 826, 862, 1138, 1311, 1535
AclWI	GGATC	8 cut(s)	157, 674, 821, 1381, 1574, 1587, 2330, 2343
AcoI	YGGCCR	1 cut(s)	2046
AcsI	RAATTY	8 cut(s)	603, 674, 879, 1129, 1432, 1486, 2245, 2283
AcuI	CTGAAG	4 cut(s)	921, 2055, 2165, 2469
AcvI	CACGTG	1 cut(s)	1985
AcyI	GRCGYC	1 cut(s)	1867
AfaI	GTAC	7 cut(s)	344, 589, 648, 753, 1284, 1547, 2365
AfiI	CCNNNNNNNGG	6 cut(s)	38, 188, 403, 982, 1876, 2625
AflII	CTTAAG	2 cut(s)	317, 2347
AjiI	CACGTC	1 cut(s)	296
AjnI	CCWGG	4 cut(s)	1582, 1869, 2048, 2338
AjuI	GAANNNNNNNTTGG	2 cut(s)	1660, 1692
Alw21I	GWGCWC	1 cut(s)	2443
Alw26I	GTCTC	2 cut(s)	320, 836
AlwI	GGATC	8 cut(s)	157, 674, 821, 1381, 1574, 1587, 2330, 2343
AlwNI	CAGNNNCTG	2 cut(s)	947, 1619
Ama87I	CYCGRG	1 cut(s)	554
AoxI	GGCC	2 cut(s)	67, 2046
ApoI	RAATTY	8 cut(s)	603, 674, 879, 1129, 1432, 1486, 2245, 2283
AseI	ATTAAT	1 cut(s)	1020
Asp700I	GAANNNNTTC	2 cut(s)	2327, 2553
AspLEI	GCGC	4 cut(s)	30, 176, 1869, 2582
AspS9I	GGNCC	3 cut(s)	68, 542, 1647
AsuC2I	CCSGG	1 cut(s)	39
AsuHPI	GGTGA	6 cut(s)	206, 719, 1001, 1037, 1517, 1521
AsuNHI	GCTAGC	1 cut(s)	1829
AvaI	CYCGRG	1 cut(s)	554
AvaII	GGWCC	2 cut(s)	542, 1647
BaeGI	GKGCMC	1 cut(s)	411
BaeI	ACNNNNGTAYC	6 cut(s)	579, 612, 638, 671, 2355, 2388
BalI	TGGCCA	1 cut(s)	2048
BamHI	GGATCC	2 cut(s)	1579, 2335
BanI	GGYRCC	2 cut(s)	958, 1866
BbrPI	CACGTG	1 cut(s)	1985
BbsI	GAAGAC	6 cut(s)	49, 342, 359, 511, 822, 1953
Bbv12I	GWGCWC	1 cut(s)	2443
BceAI	ACGGC	1 cut(s)	849
BciT130I	CCWGG	4 cut(s)	1584, 1871, 2050, 2340
BciVI	GTATCC	1 cut(s)	2383
BcnI	CCSGG	1 cut(s)	39
BcoDI	GTCTC	2 cut(s)	320, 836
BfaI	CTAG	4 cut(s)	524, 1806, 1830, 2288
BfmI	CTRYAG	2 cut(s)	1781, 2605
BfoI	RGCGCY	2 cut(s)	31, 1870
BfrI	CTTAAG	2 cut(s)	317, 2347
BfuAI	ACCTGC	2 cut(s)	1540, 1788
BfuI	GTATCC	1 cut(s)	2383
BglII	AGATCT	1 cut(s)	1924
BmcAI	AGTACT	1 cut(s)	2365
Bme1390I	CCNGG	5 cut(s)	39, 1584, 1871, 2050, 2340
Bme18I	GGWCC	2 cut(s)	542, 1647
BmeT110I	CYCGRG	1 cut(s)	554
BmgBI	CACGTC	1 cut(s)	296
BmgT120I	GGNCC	3 cut(s)	68, 542, 1647
BmiI	GGNNCC	6 cut(s)	572, 960, 1581, 1648, 1868, 2337
BmrFI	CCNGG	5 cut(s)	39, 1584, 1871, 2050, 2340
BmsI	GCATC	8 cut(s)	682, 882, 1225, 1597, 2116, 2185, 2300, 2489
BmtI	GCTAGC	1 cut(s)	1833
BoxI	GACNNNNGTC	1 cut(s)	2614
BpiI	GAAGAC	6 cut(s)	49, 342, 359, 511, 822, 1953
BplI	GAGNNNNNCTC	2 cut(s)	725, 757
BpmI	CTGGAG	3 cut(s)	732, 800, 1752
BpuEI	CTTGAG	3 cut(s)	1059, 1781, 2036
BpuMI	CCSGG	1 cut(s)	39
BsaAI	YACGTR	1 cut(s)	1985
BsaHI	GRCGYC	1 cut(s)	1867
BsaJI	CCNNGG	5 cut(s)	37, 1583, 1710, 2490, 2624
BsaXI	ACNNNNNCTCC	2 cut(s)	2234, 2264
Bsc4I	CCNNNNNNNGG	6 cut(s)	38, 188, 403, 982, 1876, 2625
Bse1I	ACTGG	4 cut(s)	749, 1598, 1881, 1977
Bse3DI	GCAATG	3 cut(s)	828, 1581, 1696
BseBI	CCWGG	4 cut(s)	1584, 1871, 2050, 2340
BseDI	CCNNGG	5 cut(s)	37, 1583, 1710, 2490, 2624
BseGI	GGATG	5 cut(s)	697, 1009, 1455, 1588, 2467
BseLI	CCNNNNNNNGG	6 cut(s)	38, 188, 403, 982, 1876, 2625
BseMI	GCAATG	3 cut(s)	828, 1581, 1696
BseMII	CTCAG	6 cut(s)	315, 719, 1164, 1392, 2552, 2613
BseNI	ACTGG	4 cut(s)	749, 1598, 1881, 1977
BseRI	GAGGAG	5 cut(s)	644, 1115, 1473, 2033, 2255
BseSI	GKGCMC	1 cut(s)	411
BseYI	CCCAGC	1 cut(s)	1038
BsgI	GTGCAG	1 cut(s)	751
BshFI	GGCC	2 cut(s)	69, 2048
BshNI	GGYRCC	2 cut(s)	958, 1866
BsiHKAI	GWGCWC	1 cut(s)	2443
BsiHKCI	CYCGRG	1 cut(s)	554
BsiSI	CCGG	1 cut(s)	39
BslFI	GGGAC	1 cut(s)	2602
BslI	CCNNNNNNNGG	6 cut(s)	38, 188, 403, 982, 1876, 2625
BsmAI	GTCTC	2 cut(s)	320, 836
BsmBI	CGTCTC	1 cut(s)	836
BsmFI	GGGAC	1 cut(s)	2602
BsnI	GGCC	2 cut(s)	69, 2048
BsoBI	CYCGRG	1 cut(s)	554
Bsp1286I	GDGCHC	2 cut(s)	411, 2443
Bsp1407I	TGTACA	1 cut(s)	1282
Bsp19I	CCATGG	2 cut(s)	1710, 2624
BspACI	CCGC	8 cut(s)	34, 118, 304, 826, 862, 1138, 1311, 1535
BspANI	GGCC	2 cut(s)	69, 2048
BspCNI	CTCAG	6 cut(s)	316, 720, 1163, 1393, 2551, 2612
BspHI	TCATGA	2 cut(s)	916, 2218
BspLI	GGNNCC	6 cut(s)	572, 960, 1581, 1648, 1868, 2337
BspMAI	CTGCAG	1 cut(s)	1785
BspMI	ACCTGC	2 cut(s)	1540, 1788
BspOI	GCTAGC	1 cut(s)	1833
BspPI	GGATC	8 cut(s)	157, 674, 821, 1381, 1574, 1587, 2330, 2343
BspQI	GCTCTTC	2 cut(s)	570, 1734
BspT107I	GGYRCC	2 cut(s)	958, 1866
BspTI	CTTAAG	2 cut(s)	317, 2347
BsrDI	GCAATG	3 cut(s)	828, 1581, 1696
BsrGI	TGTACA	1 cut(s)	1282
BsrI	ACTGG	4 cut(s)	749, 1598, 1881, 1977
BssECI	CCNNGG	5 cut(s)	37, 1583, 1710, 2490, 2624
BssNI	GRCGYC	1 cut(s)	1867
BssT1I	CCWWGG	3 cut(s)	1710, 2490, 2624
Bst2UI	CCWGG	4 cut(s)	1584, 1871, 2050, 2340
Bst4CI	ACNGT	6 cut(s)	55, 277, 615, 1289, 1971, 2138
Bst6I	CTCTTC	3 cut(s)	570, 910, 1734
BstACI	GRCGYC	1 cut(s)	1867
BstAFI	CTTAAG	2 cut(s)	317, 2347
BstAPI	GCANNNNNTGC	2 cut(s)	947, 1061
BstAUI	TGTACA	1 cut(s)	1282
BstBAI	YACGTR	1 cut(s)	1985
BstC8I	GCNNGC	6 cut(s)	306, 519, 952, 1321, 1831, 1851
BstDEI	CTNAG	8 cut(s)	324, 728, 1150, 1401, 1928, 1998, 2538, 2599
BstDSI	CCRYGG	2 cut(s)	1710, 2624
BstEII	GGTNACC	1 cut(s)	1226
BstF5I	GGATG	5 cut(s)	697, 1009, 1455, 1588, 2467
BstH2I	RGCGCY	2 cut(s)	31, 1870
BstHHI	GCGC	4 cut(s)	30, 176, 1869, 2582
BstMAI	GTCTC	2 cut(s)	320, 836
BstNI	CCWGG	4 cut(s)	1584, 1871, 2050, 2340
BstNSI	RCATGY	3 cut(s)	371, 1960, 2133
BstPAI	GACNNNNGTC	1 cut(s)	2614
BstPI	GGTNACC	1 cut(s)	1226
BstSCI	CCNGG	5 cut(s)	37, 1582, 1869, 2048, 2338
BstSFI	CTRYAG	2 cut(s)	1781, 2605
BstSLI	GKGCMC	1 cut(s)	411
BstV2I	GAAGAC	6 cut(s)	49, 342, 359, 511, 822, 1953
BstX2I	RGATCY	3 cut(s)	1579, 1924, 2335
BstYI	RGATCY	3 cut(s)	1579, 1924, 2335
BsuI	GTATCC	1 cut(s)	2383
BsuRI	GGCC	2 cut(s)	69, 2048
BtgI	CCRYGG	2 cut(s)	1710, 2624
BtrI	CACGTC	1 cut(s)	296
BtsCI	GGATG	5 cut(s)	697, 1009, 1455, 1588, 2467
BtsI	GCAGTG	4 cut(s)	768, 1186, 1330, 1635
BtsIMutI	CAGTG	6 cut(s)	768, 1186, 1294, 1330, 1635, 1874
BveI	ACCTGC	2 cut(s)	1540, 1788
Cac8I	GCNNGC	6 cut(s)	306, 519, 952, 1321, 1831, 1851
CaiI	CAGNNNCTG	2 cut(s)	947, 1619
CciI	TCATGA	2 cut(s)	916, 2218
CfoI	GCGC	4 cut(s)	30, 176, 1869, 2582
Cfr13I	GGNCC	3 cut(s)	68, 542, 1647
CseI	GACGC	1 cut(s)	467
Csp6I	GTAC	7 cut(s)	343, 588, 647, 752, 1283, 1546, 2364
CviQI	GTAC	7 cut(s)	343, 588, 647, 752, 1283, 1546, 2364
DdeI	CTNAG	8 cut(s)	324, 728, 1150, 1401, 1928, 1998, 2538, 2599
DinI	GGCGCC	1 cut(s)	1868
DraI	TTTAAA	3 cut(s)	156, 885, 2571
EaeI	YGGCCR	1 cut(s)	2046
Eam1104I	CTCTTC	3 cut(s)	570, 910, 1734
EarI	CTCTTC	3 cut(s)	570, 910, 1734
Eco130I	CCWWGG	3 cut(s)	1710, 2490, 2624
Eco47I	GGWCC	2 cut(s)	542, 1647
Eco57I	CTGAAG	4 cut(s)	921, 2055, 2165, 2469
Eco72I	CACGTG	1 cut(s)	1985
Eco88I	CYCGRG	1 cut(s)	554
Eco91I	GGTNACC	1 cut(s)	1226
EcoO109I	RGGNCCY	1 cut(s)	542
EcoO65I	GGTNACC	1 cut(s)	1226
EcoRI	GAATTC	1 cut(s)	1432
EcoRII	CCWGG	4 cut(s)	1582, 1869, 2048, 2338
EcoT14I	CCWWGG	3 cut(s)	1710, 2490, 2624
EcoT22I	ATGCAT	2 cut(s)	897, 2131
EgeI	GGCGCC	1 cut(s)	1868
EheI	GGCGCC	1 cut(s)	1868
ErhI	CCWWGG	3 cut(s)	1710, 2490, 2624
Esp3I	CGTCTC	1 cut(s)	836
FaqI	GGGAC	1 cut(s)	2602
FauI	CCCGC	3 cut(s)	41, 297, 1528
FokI	GGATG	5 cut(s)	704, 1016, 1462, 1575, 2454
FspBI	CTAG	4 cut(s)	524, 1806, 1830, 2288
GlaI	GCGC	4 cut(s)	29, 175, 1868, 2581
GsaI	CCCAGC	1 cut(s)	1042
GsuI	CTGGAG	3 cut(s)	732, 800, 1752
HaeII	RGCGCY	2 cut(s)	31, 1870
HaeIII	GGCC	2 cut(s)	69, 2048
HapII	CCGG	1 cut(s)	39
HgaI	GACGC	1 cut(s)	467
HhaI	GCGC	4 cut(s)	30, 176, 1869, 2582
Hin1I	GRCGYC	1 cut(s)	1867
Hin6I	GCGC	4 cut(s)	28, 174, 1867, 2580
HinP1I	GCGC	4 cut(s)	28, 174, 1867, 2580
HindIII	AAGCTT	1 cut(s)	1851
HpaII	CCGG	1 cut(s)	39
HphI	GGTGA	6 cut(s)	206, 719, 1001, 1037, 1517, 1521
Hpy166II	GTNNAC	3 cut(s)	1283, 1285, 1982
Hpy188I	TCNGA	9 cut(s)	334, 425, 729, 813, 1195, 1366, 1609, 1755, 2125
Hpy8I	GTNNAC	3 cut(s)	1283, 1285, 1982
Hpy99I	CGWCG	2 cut(s)	1199, 1445
HpyAV	CCTTC	5 cut(s)	443, 555, 593, 945, 1439
HpyCH4III	ACNGT	6 cut(s)	55, 277, 615, 1289, 1971, 2138
HpyCH4IV	ACGT	7 cut(s)	295, 723, 845, 1440, 1984, 2148, 2533
HpyF3I	CTNAG	8 cut(s)	324, 728, 1150, 1401, 1928, 1998, 2538, 2599
HpySE526I	ACGT	7 cut(s)	295, 723, 845, 1440, 1984, 2148, 2533
Hsp92I	GRCGYC	1 cut(s)	1867
HspAI	GCGC	4 cut(s)	28, 174, 1867, 2580
KasI	GGCGCC	1 cut(s)	1866
LguI	GCTCTTC	2 cut(s)	570, 1734
LmnI	GCTCC	3 cut(s)	41, 388, 1616
LweI	GCATC	8 cut(s)	682, 882, 1225, 1597, 2116, 2185, 2300, 2489
MaeI	CTAG	4 cut(s)	524, 1806, 1830, 2288
MaeII	ACGT	7 cut(s)	295, 723, 845, 1440, 1984, 2148, 2533
MaeIII	GTNAC	5 cut(s)	1226, 1325, 1639, 2132, 2138
MflI	RGATCY	3 cut(s)	1579, 1924, 2335
MhlI	GDGCHC	2 cut(s)	411, 2443
MlsI	TGGCCA	1 cut(s)	2048
MluNI	TGGCCA	1 cut(s)	2048
Mly113I	GGCGCC	1 cut(s)	1867
MlyI	GAGTC	1 cut(s)	2591
MmeI	TCCRAC	4 cut(s)	481, 981, 1218, 1351
Mox20I	TGGCCA	1 cut(s)	2048
Mph1103I	ATGCAT	2 cut(s)	897, 2131
MroXI	GAANNNNTTC	2 cut(s)	2327, 2553
MscI	TGGCCA	1 cut(s)	2048
MslI	CAYNNNNRTG	1 cut(s)	915
Msp20I	TGGCCA	1 cut(s)	2048
MspA1I	CMGCKG	4 cut(s)	1154, 1311, 1400, 2405
MspCI	CTTAAG	2 cut(s)	317, 2347
MspI	CCGG	1 cut(s)	39
MspR9I	CCNGG	5 cut(s)	39, 1584, 1871, 2050, 2340
MvaI	CCWGG	4 cut(s)	1584, 1871, 2050, 2340
NarI	GGCGCC	1 cut(s)	1867
NciI	CCSGG	1 cut(s)	39
NcoI	CCATGG	2 cut(s)	1710, 2624
NheI	GCTAGC	1 cut(s)	1829
NlaIV	GGNNCC	6 cut(s)	572, 960, 1581, 1648, 1868, 2337
NmuCI	GTSAC	2 cut(s)	1325, 2132
NsiI	ATGCAT	2 cut(s)	897, 2131
NspI	RCATGY	3 cut(s)	371, 1960, 2133
PaeR7I	CTCGAG	1 cut(s)	554
PagI	TCATGA	2 cut(s)	916, 2218
PaqCI	CACCTGC	1 cut(s)	1540
PciSI	GCTCTTC	2 cut(s)	570, 1734
PdmI	GAANNNNTTC	2 cut(s)	2327, 2553
PflFI	GACNNNGTC	1 cut(s)	2600
PleI	GAGTC	1 cut(s)	2591
PluTI	GGCGCC	1 cut(s)	1870
PmaCI	CACGTG	1 cut(s)	1985
PmlI	CACGTG	1 cut(s)	1985
PpsI	GAGTC	1 cut(s)	2591
Ppu21I	YACGTR	1 cut(s)	1985
PpuMI	RGGWCCY	1 cut(s)	542
PshAI	GACNNNNGTC	1 cut(s)	2614
PshBI	ATTAAT	1 cut(s)	1020
PsiI	TTATAA	2 cut(s)	789, 1341
Psp5II	RGGWCCY	1 cut(s)	542
Psp6I	CCWGG	4 cut(s)	1582, 1869, 2048, 2338
PspCI	CACGTG	1 cut(s)	1985
PspEI	GGTNACC	1 cut(s)	1226
PspFI	CCCAGC	1 cut(s)	1038
PspGI	CCWGG	4 cut(s)	1582, 1869, 2048, 2338
PspN4I	GGNNCC	6 cut(s)	572, 960, 1581, 1648, 1868, 2337
PspPI	GGNCC	3 cut(s)	68, 542, 1647
PspPPI	RGGWCCY	1 cut(s)	542
PsrI	GAACNNNNNNTAC	2 cut(s)	630, 662
PstI	CTGCAG	1 cut(s)	1785
PstNI	CAGNNNCTG	2 cut(s)	947, 1619
PsuI	RGATCY	3 cut(s)	1579, 1924, 2335
PsyI	GACNNNGTC	1 cut(s)	2600
PvuII	CAGCTG	3 cut(s)	1154, 1400, 2405
RsaI	GTAC	7 cut(s)	344, 589, 648, 753, 1284, 1547, 2365
RsaNI	GTAC	7 cut(s)	343, 588, 647, 752, 1283, 1546, 2364
RseI	CAYNNNNRTG	1 cut(s)	915
SapI	GCTCTTC	2 cut(s)	570, 1734
Sau96I	GGNCC	3 cut(s)	68, 542, 1647
ScaI	AGTACT	1 cut(s)	2365
SchI	GAGTC	1 cut(s)	2591
ScrFI	CCNGG	5 cut(s)	39, 1584, 1871, 2050, 2340
SduI	GDGCHC	2 cut(s)	411, 2443
SfaNI	GCATC	8 cut(s)	682, 882, 1225, 1597, 2116, 2185, 2300, 2489
SfcI	CTRYAG	2 cut(s)	1781, 2605
SfoI	GGCGCC	1 cut(s)	1868
Sfr274I	CTCGAG	1 cut(s)	554
SinI	GGWCC	2 cut(s)	542, 1647
SlaI	CTCGAG	1 cut(s)	554
SmiI	ATTTAAAT	1 cut(s)	2571
SmiMI	CAYNNNNRTG	1 cut(s)	915
SmlI	CTYRAG	6 cut(s)	317, 554, 1074, 1760, 2015, 2347
SmoI	CTYRAG	6 cut(s)	317, 554, 1074, 1760, 2015, 2347
SsiI	CCGC	8 cut(s)	34, 118, 304, 826, 862, 1138, 1311, 1535
SspDI	GGCGCC	1 cut(s)	1866
SspI	AATATT	1 cut(s)	837
SspMI	CTAG	4 cut(s)	524, 1806, 1830, 2288
StyD4I	CCNGG	5 cut(s)	37, 1582, 1869, 2048, 2338
StyI	CCWWGG	3 cut(s)	1710, 2490, 2624
SwaI	ATTTAAAT	1 cut(s)	2571
TaaI	ACNGT	6 cut(s)	55, 277, 615, 1289, 1971, 2138
TaiI	ACGT	7 cut(s)	298, 726, 848, 1443, 1987, 2151, 2536
TaqI	TCGA	7 cut(s)	555, 799, 925, 1145, 1162, 1443, 1722
TatI	WGTACW	2 cut(s)	1282, 2363
TscAI	CASTG	6 cut(s)	775, 1186, 1294, 1330, 1635, 1881
TseFI	GTSAC	2 cut(s)	1325, 2132
Tsp45I	GTSAC	2 cut(s)	1325, 2132
TspDTI	ATGAA	9 cut(s)	67, 672, 944, 1260, 1497, 2235, 2320, 2409, 2546
TspGWI	ACGGA	2 cut(s)	151, 2239
TspRI	CASTG	6 cut(s)	775, 1186, 1294, 1330, 1635, 1881
Tth111I	GACNNNGTC	1 cut(s)	2600
Vha464I	CTTAAG	2 cut(s)	317, 2347
VpaK11BI	GGWCC	2 cut(s)	542, 1647
VspI	ATTAAT	1 cut(s)	1020
XapI	RAATTY	8 cut(s)	603, 674, 879, 1129, 1432, 1486, 2245, 2283
XceI	RCATGY	3 cut(s)	371, 1960, 2133
XhoI	CTCGAG	1 cut(s)	554
XmnI	GAANNNNTTC	2 cut(s)	2327, 2553
XspI	CTAG	4 cut(s)	524, 1806, 1830, 2288
ZrmI	AGTACT	1 cut(s)	2365
Zsp2I	ATGCAT	2 cut(s)	897, 2131

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.