Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0006813

Physical Location & Seq

Genomic Coordinates Reverse (-)

52260378 .. 52274914

Gene Structure 14537 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000034705

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2631 bp

ATGTCGGCTTCTTTAGCTGCTTTTGAGCGCCCCCGCCCCGGAGCTTCTAACACGGTCTTCAAGAGTGGCCCATTATTCATATCTTCAAAAGGAATAGGGTGGAAGTCTTGGAAGAAGCGGTGGTTTATCCTCACACGGACTTCTTTGGTTTTCTTTAAAAATGATCCTAGTGCACTTCCACAAAAAGGTGGTGAAGTATATTTGACTTTAGGAGGCATTGACCTGAACAATTCTGGGAGTGTTGTTGTTAGAGAAGATAAAAAGCTGCTGACTGTTTTATTTCCTGATGGACGTGATGGGCGGGCTTTCACTCTTAAGGCTGAGACATCAGAAGACTTGTACGAGTGGAAGACAGCCCTTGAACATGCCCTTGCACAAGCTCCAAGTGCCGCCCTTGTCATGGGGCACAATGGGATTTTCAGAAATGACACAAATGATACAATAGAAGGGTCTTTCCATCAATGGAGGGATAAGCGTCCTGTTAAATCTTTGGTCGTTGGAAGACCAATTCTGCTTGCCCTAGAAGATATTGATGGAGGTCCTTCTTTCCTTGAGAAAGCTCTTCGGTTCCTTGAAAAGTTTGGTACTAAAGTTGAAGGAATTTTGCGACAGTCTGCTGATGTTGAGGAGGTAGAACGCAGGGTACAAGAGTATGAACAAGGCAAGACTGAATTTGATCCTGATGAGGATGCTCATGTTGTTGGTGATTGTGTCAAGCACGTTCTGAGAGAACTGCCCTCATCTCCAGTACCAGCTTCTTGCTGCACTGCCTTGCTGGAGGCTTATAAAATTGATCGAAAGGAAGCTCGGATCAATGCAATGCGGTCTTCAATATTAGAGACATTTCCTGAACCAAACCGCCGTTTATTACAAAGAATTTTAAAGATGATGCATACCATCTCTTCTCACTCTCATGAGAATCGAATGAATCCATCTGCAGTTGCTGCTTGCATGGCACCCTTGCTCTTACGCCCTCTATTGGCTGGTGAATGTGAGTTGGATGATGACTTTGATATTAATGGTGATAATTCTGCCCAGCTTCTTGCTGCTGCAAATGCTGCCAATAATGCTCAAGCAATCATCACTACGCTTTTGGAGGAGTTTGAAAACATTTTTGATGATGAGAATTTACTGCGGTGTTCGATCTCAGCTGATTCTCGAATTGAAAATAGTGGCAGCGATGATTCGTCCGACGATGACAACCTTGATATAAAAGATAATGGTTACCATGATGCCGAAAATGAAGTTGATGTGGAAACAGATGATGACCAAGATCGTGTACACAGTGGGAAATTGAGTGAAAGCAGCGGTTATGCTGGCAGTGACCTCTATGATTATAAGGCATTTGGGGCTGATGATTCTGATGTTGGATCGCCTAATGGAAATCATGCTTCAGCTGAGAGTTCAAAATCAGTTGTTCCTCAACAGAATCCAAACGTCGAAGGGATGGATAGAGGAGGCAAACAGATAAAAGAAAATGAAAATTCAATTAGTGAGAATGGTGAAACGAGCATACCAAGCACATCACCTGCGGGAGAATCGTACAGATCAATGGGAGAGATTCTATCAGCAATGGATCCAGGGCATCCTTTACCAGTTGTGTCTGAATCAGGAGCTGTTAAGGCAGTGGGTAAAGTAACAGGGTCCAATCATAGTGCAAAGCGAACAGCATTTTGGGGAAGAAGCAATGCAAGAAAGACACTATCCATGGAATCAGTCGATTCTTCTGGAGAAGAAGAGCTTTCTATTCAGAGGCTTGAGATTGCTAAAAATGACCTGCAGCACAGGATTGCAAAAGAGGCTAGAGGAAATGCAATTTTACAAGCTAGCTTAGAGAGAAGAAAGCAAGCTTTACATGAGAGGCGCCTGGCACTGGAACAAGATGTTTCAAGATTACAAGAACAGCTACAAGCTGAAAGAGATCTTAGAGCTGCATTAGAGGTTGGTTTGAGCATGTCTTCTGGACAGTTAGCCAGTTCACGTGGCATGGATTCTAAGACAAGAGCTGAACTTGAGGAGATAGCTCTTGCTGAAGCAGATGTGGCCAGGTTGAAACAGAAAGTTGCAGAACTCCACCATCAACTTAATCAGCAGCGACAGAATCATTATGGTTCTCTCTCTGATGCATGTGACCGTTACCAGAACGTCCAAAATCATAATTCTCAACAGAGGTTTCTTCAGCAAGATTTTGATGCAACCCTCGCCTTTTGTAATCATGAACGGAAACAGAGGAATGAGGAGAATTTGTTGGGGGCAGATTGGAGGAATATCAAAGGACAAATTCTAGCATCTGGAAGTAGCACAAGGCAGCCATCAAGGAAGCAATTCATGGATCCAGTTAGCTTAAGTGAATCCAAAAGTACTGATGTATCCACAAGTTTGTCTGTTGATGAGCTTTCTGCTGTTGATTCTGCTTCCATTCCCTCCACTTCAAGAGCAGCAGAGGTGGTGGATTATCCAAGACATCCATCAACAGCATCTTCAGCCTTGGTAGAATTAACAACTCGTCTTGATTTCTTCAAGGAAAGACGTTCTCAGCTAATGGAACAGCTTCATAACCTTGATTTAAATTATGGCGCAACATCTTTGCAAGACTCAGTCTATAGACCATCGTCCCCACCATGGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0000919 GO:0003674 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006996 GO:0007049 GO:0008047 GO:0008150 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009832 GO:0009920 GO:0009987 GO:0016020 GO:0016043 GO:0022402 GO:0022607 GO:0030234 GO:0030695 GO:0031976 GO:0031984 GO:0032506 GO:0034357 GO:0042546 GO:0042651 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043547 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0050790 GO:0051301 GO:0051336 GO:0051345 GO:0055035 GO:0060589 GO:0061640 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0098772 GO:0140014 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lzipper-MIP1 PH RhoGAP

Protein Analysis

877

Amino Acids

96.72

Weight (kDa)

5.2

Isoelectric Point (pI)

50.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH	PF00169	20 - 124	2.5e-13	PH domain
RhoGAP	PF00620	181 - 325	2.8e-34	RhoGAP domain
Lzipper-MIP1	PF14389	618 - 698	1.9e-21	Leucine-zipper of ternary complex factor MIP1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006813)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19390 AT5G19390 AT5G19390 AT5G19390
fragaria_vesca	FvH4_3g27830 FvH4_3g27830 FvH4_3g27830 FvH4_3g27830
malus_domestica	MD03G1165400.v1.1 MD11G1184700.v1.1
prunus_persica	Prupe.4G265200_v2.0.a1 Prupe.4G265200_v2.0.a1 Prupe.4G265200_v2.0.a1 Prupe.4G265300_v2.0.a1
pyrus_communis	pycom03g11990 pycom12g08810
rosa_chinensis	RchiOBHm_Chr5g0050541
rosa_laevigata	RLG00000034705
rosa_multiflora	Rmu_sc0004301.1_g000009
rosa_roxburghii	Rroxscaffold_1G00030350
rosa_rugosa	Rorug05G0261400
rosa_samantha	Rh5AG333300 Rh5BG343200 Rh5CG370800 Rh5DG355900
rosa_wichuraiana	Rw5G031480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	786, 1338
AarI	CACCTGC	1 cut(s)	1537
Acc36I	ACCTGC	2 cut(s)	1537, 1785
AccB1I	GGYRCC	2 cut(s)	955, 1863
AciI	CCGC	9 cut(s)	34, 118, 301, 390, 823, 859, 1135, 1308, 1532
AclWI	GGATC	8 cut(s)	158, 671, 818, 1378, 1571, 1584, 2327, 2340
AcoI	YGGCCR	1 cut(s)	2043
AcsI	RAATTY	7 cut(s)	600, 671, 876, 1126, 1483, 2242, 2280
AcuI	CTGAAG	4 cut(s)	1377, 2052, 2162, 2466
AcvI	CACGTG	1 cut(s)	1982
AcyI	GRCGYC	1 cut(s)	1864
AfaI	GTAC	7 cut(s)	341, 586, 645, 750, 1281, 1544, 2362
AfiI	CCNNNNNNNGG	6 cut(s)	38, 185, 400, 979, 1873, 2622
AflII	CTTAAG	2 cut(s)	314, 2344
AjiI	CACGTC	1 cut(s)	293
AjnI	CCWGG	3 cut(s)	1579, 1866, 2045
AjuI	GAANNNNNNNTTGG	2 cut(s)	1657, 1689
Alw21I	GWGCWC	1 cut(s)	175
Alw26I	GTCTC	2 cut(s)	317, 833
Alw44I	GTGCAC	1 cut(s)	171
AlwI	GGATC	8 cut(s)	158, 671, 818, 1378, 1571, 1584, 2327, 2340
AlwNI	CAGNNNCTG	2 cut(s)	944, 1616
AoxI	GGCC	2 cut(s)	67, 2043
ApaLI	GTGCAC	1 cut(s)	171
ApoI	RAATTY	7 cut(s)	600, 671, 876, 1126, 1483, 2242, 2280
AseI	ATTAAT	1 cut(s)	1017
Asp700I	GAANNNNTTC	2 cut(s)	2324, 2550
AspLEI	GCGC	3 cut(s)	30, 1866, 2579
AspS9I	GGNCC	3 cut(s)	68, 539, 1644
AsuC2I	CCSGG	1 cut(s)	39
AsuHPI	GGTGA	6 cut(s)	203, 716, 998, 1034, 1514, 1518
AsuNHI	GCTAGC	1 cut(s)	1826
AvaII	GGWCC	2 cut(s)	539, 1644
BaeGI	GKGCMC	2 cut(s)	175, 408
BaeI	ACNNNNGTAYC	6 cut(s)	576, 609, 635, 668, 2352, 2385
BalI	TGGCCA	1 cut(s)	2045
BamHI	GGATCC	2 cut(s)	1576, 2332
BanI	GGYRCC	2 cut(s)	955, 1863
BbrPI	CACGTG	1 cut(s)	1982
BbsI	GAAGAC	6 cut(s)	49, 339, 356, 508, 819, 1950
Bbv12I	GWGCWC	1 cut(s)	175
BceAI	ACGGC	1 cut(s)	846
BciT130I	CCWGG	3 cut(s)	1581, 1868, 2047
BciVI	GTATCC	1 cut(s)	2380
BcnI	CCSGG	1 cut(s)	39
BcoDI	GTCTC	2 cut(s)	317, 833
BfaI	CTAG	5 cut(s)	168, 521, 1803, 1827, 2285
BfmI	CTRYAG	3 cut(s)	936, 1778, 2602
BfoI	RGCGCY	2 cut(s)	31, 1867
BfrI	CTTAAG	2 cut(s)	314, 2344
BfuAI	ACCTGC	2 cut(s)	1537, 1785
BfuI	GTATCC	1 cut(s)	2380
BglII	AGATCT	1 cut(s)	1921
BmcAI	AGTACT	1 cut(s)	2362
Bme1390I	CCNGG	4 cut(s)	39, 1581, 1868, 2047
Bme18I	GGWCC	2 cut(s)	539, 1644
BmgBI	CACGTC	1 cut(s)	293
BmgT120I	GGNCC	3 cut(s)	68, 539, 1644
BmiI	GGNNCC	6 cut(s)	569, 957, 1578, 1645, 1865, 2334
BmrFI	CCNGG	4 cut(s)	39, 1581, 1868, 2047
BmsI	GCATC	8 cut(s)	679, 879, 1222, 1594, 2113, 2182, 2297, 2486
BmtI	GCTAGC	1 cut(s)	1830
BoxI	GACNNNNGTC	1 cut(s)	2611
BpiI	GAAGAC	6 cut(s)	49, 339, 356, 508, 819, 1950
BpmI	CTGGAG	3 cut(s)	729, 797, 1749
BpuEI	CTTGAG	4 cut(s)	572, 1056, 1778, 2033
BpuMI	CCSGG	1 cut(s)	39
BsaAI	YACGTR	1 cut(s)	1982
BsaHI	GRCGYC	1 cut(s)	1864
BsaJI	CCNNGG	5 cut(s)	37, 1580, 1707, 2487, 2621
BsaXI	ACNNNNNCTCC	2 cut(s)	2231, 2261
Bsc4I	CCNNNNNNNGG	6 cut(s)	38, 185, 400, 979, 1873, 2622
Bse1I	ACTGG	5 cut(s)	746, 1595, 1878, 1974, 2336
Bse3DI	GCAATG	3 cut(s)	825, 1578, 1693
BseBI	CCWGG	3 cut(s)	1581, 1868, 2047
BseDI	CCNNGG	5 cut(s)	37, 1580, 1707, 2487, 2621
BseGI	GGATG	5 cut(s)	694, 1006, 1452, 1585, 2464
BseLI	CCNNNNNNNGG	6 cut(s)	38, 185, 400, 979, 1873, 2622
BseMI	GCAATG	3 cut(s)	825, 1578, 1693
BseMII	CTCAG	6 cut(s)	312, 716, 1161, 1389, 2549, 2610
BseNI	ACTGG	5 cut(s)	746, 1595, 1878, 1974, 2336
BseRI	GAGGAG	5 cut(s)	641, 1112, 1470, 2030, 2252
BseSI	GKGCMC	2 cut(s)	175, 408
BseYI	CCCAGC	1 cut(s)	1035
BsgI	GTGCAG	1 cut(s)	748
BshFI	GGCC	2 cut(s)	69, 2045
BshNI	GGYRCC	2 cut(s)	955, 1863
BsiHKAI	GWGCWC	1 cut(s)	175
BsiSI	CCGG	1 cut(s)	39
BslFI	GGGAC	1 cut(s)	2599
BslI	CCNNNNNNNGG	6 cut(s)	38, 185, 400, 979, 1873, 2622
BsmAI	GTCTC	2 cut(s)	317, 833
BsmFI	GGGAC	1 cut(s)	2599
BsnI	GGCC	2 cut(s)	69, 2045
Bsp1286I	GDGCHC	2 cut(s)	175, 408
Bsp1407I	TGTACA	1 cut(s)	1279
Bsp19I	CCATGG	2 cut(s)	1707, 2621
BspACI	CCGC	9 cut(s)	34, 118, 301, 390, 823, 859, 1135, 1308, 1532
BspANI	GGCC	2 cut(s)	69, 2045
BspCNI	CTCAG	6 cut(s)	313, 717, 1160, 1390, 2548, 2609
BspHI	TCATGA	2 cut(s)	913, 2215
BspLI	GGNNCC	6 cut(s)	569, 957, 1578, 1645, 1865, 2334
BspMAI	CTGCAG	2 cut(s)	940, 1782
BspMI	ACCTGC	2 cut(s)	1537, 1785
BspOI	GCTAGC	1 cut(s)	1830
BspPI	GGATC	8 cut(s)	158, 671, 818, 1378, 1571, 1584, 2327, 2340
BspQI	GCTCTTC	2 cut(s)	567, 1731
BspT107I	GGYRCC	2 cut(s)	955, 1863
BspTI	CTTAAG	2 cut(s)	314, 2344
BsrDI	GCAATG	3 cut(s)	825, 1578, 1693
BsrGI	TGTACA	1 cut(s)	1279
BsrI	ACTGG	5 cut(s)	746, 1595, 1878, 1974, 2336
BssECI	CCNNGG	5 cut(s)	37, 1580, 1707, 2487, 2621
BssNI	GRCGYC	1 cut(s)	1864
BssT1I	CCWWGG	3 cut(s)	1707, 2487, 2621
Bst2UI	CCWGG	3 cut(s)	1581, 1868, 2047
Bst4CI	ACNGT	6 cut(s)	55, 274, 612, 1286, 1968, 2135
Bst6I	CTCTTC	3 cut(s)	567, 907, 1731
BstACI	GRCGYC	1 cut(s)	1864
BstAFI	CTTAAG	2 cut(s)	314, 2344
BstAPI	GCANNNNNTGC	2 cut(s)	944, 1058
BstAUI	TGTACA	1 cut(s)	1279
BstBAI	YACGTR	1 cut(s)	1982
BstC8I	GCNNGC	6 cut(s)	303, 516, 949, 1318, 1828, 1848
BstDEI	CTNAG	9 cut(s)	321, 725, 1147, 1398, 1831, 1925, 1995, 2535, 2596
BstDSI	CCRYGG	2 cut(s)	1707, 2621
BstEII	GGTNACC	1 cut(s)	1223
BstF5I	GGATG	5 cut(s)	694, 1006, 1452, 1585, 2464
BstH2I	RGCGCY	2 cut(s)	31, 1867
BstHHI	GCGC	3 cut(s)	30, 1866, 2579
BstMAI	GTCTC	2 cut(s)	317, 833
BstNI	CCWGG	3 cut(s)	1581, 1868, 2047
BstNSI	RCATGY	3 cut(s)	368, 1957, 2130
BstPAI	GACNNNNGTC	1 cut(s)	2611
BstPI	GGTNACC	1 cut(s)	1223
BstSCI	CCNGG	4 cut(s)	37, 1579, 1866, 2045
BstSFI	CTRYAG	3 cut(s)	936, 1778, 2602
BstSLI	GKGCMC	2 cut(s)	175, 408
BstV2I	GAAGAC	6 cut(s)	49, 339, 356, 508, 819, 1950
BstX2I	RGATCY	3 cut(s)	1576, 1921, 2332
BstYI	RGATCY	3 cut(s)	1576, 1921, 2332
BsuI	GTATCC	1 cut(s)	2380
BsuRI	GGCC	2 cut(s)	69, 2045
BtgI	CCRYGG	2 cut(s)	1707, 2621
BtgZI	GCGATG	1 cut(s)	1194
BtrI	CACGTC	1 cut(s)	293
BtsCI	GGATG	5 cut(s)	694, 1006, 1452, 1585, 2464
BtsI	GCAGTG	3 cut(s)	765, 1327, 1632
BtsIMutI	CAGTG	5 cut(s)	765, 1291, 1327, 1632, 1871
BveI	ACCTGC	2 cut(s)	1537, 1785
Cac8I	GCNNGC	6 cut(s)	303, 516, 949, 1318, 1828, 1848
CaiI	CAGNNNCTG	2 cut(s)	944, 1616
CciI	TCATGA	2 cut(s)	913, 2215
CfoI	GCGC	3 cut(s)	30, 1866, 2579
Cfr13I	GGNCC	3 cut(s)	68, 539, 1644
CseI	GACGC	1 cut(s)	464
Csp6I	GTAC	7 cut(s)	340, 585, 644, 749, 1280, 1543, 2361
CviQI	GTAC	7 cut(s)	340, 585, 644, 749, 1280, 1543, 2361
DdeI	CTNAG	9 cut(s)	321, 725, 1147, 1398, 1831, 1925, 1995, 2535, 2596
DinI	GGCGCC	1 cut(s)	1865
DraI	TTTAAA	3 cut(s)	157, 882, 2568
EaeI	YGGCCR	1 cut(s)	2043
Eam1104I	CTCTTC	3 cut(s)	567, 907, 1731
EarI	CTCTTC	3 cut(s)	567, 907, 1731
Eco130I	CCWWGG	3 cut(s)	1707, 2487, 2621
Eco47I	GGWCC	2 cut(s)	539, 1644
Eco57I	CTGAAG	4 cut(s)	1377, 2052, 2162, 2466
Eco72I	CACGTG	1 cut(s)	1982
Eco91I	GGTNACC	1 cut(s)	1223
EcoO109I	RGGNCCY	1 cut(s)	539
EcoO65I	GGTNACC	1 cut(s)	1223
EcoRII	CCWGG	3 cut(s)	1579, 1866, 2045
EcoT14I	CCWWGG	3 cut(s)	1707, 2487, 2621
EcoT22I	ATGCAT	2 cut(s)	894, 2128
EgeI	GGCGCC	1 cut(s)	1865
EheI	GGCGCC	1 cut(s)	1865
ErhI	CCWWGG	3 cut(s)	1707, 2487, 2621
FalI	AAGNNNNNCTT	2 cut(s)	1725, 1757
FaqI	GGGAC	1 cut(s)	2599
FauI	CCCGC	3 cut(s)	41, 294, 1525
FokI	GGATG	5 cut(s)	701, 1013, 1459, 1572, 2451
FspBI	CTAG	5 cut(s)	168, 521, 1803, 1827, 2285
GlaI	GCGC	3 cut(s)	29, 1865, 2578
GsaI	CCCAGC	1 cut(s)	1039
GsuI	CTGGAG	3 cut(s)	729, 797, 1749
HaeII	RGCGCY	2 cut(s)	31, 1867
HaeIII	GGCC	2 cut(s)	69, 2045
HapII	CCGG	1 cut(s)	39
HgaI	GACGC	1 cut(s)	464
HhaI	GCGC	3 cut(s)	30, 1866, 2579
Hin1I	GRCGYC	1 cut(s)	1864
Hin6I	GCGC	3 cut(s)	28, 1864, 2577
HinP1I	GCGC	3 cut(s)	28, 1864, 2577
HindIII	AAGCTT	1 cut(s)	1848
HpaII	CCGG	1 cut(s)	39
HphI	GGTGA	6 cut(s)	203, 716, 998, 1034, 1514, 1518
Hpy166II	GTNNAC	4 cut(s)	173, 1280, 1282, 1979
Hpy188I	TCNGA	9 cut(s)	331, 422, 726, 810, 1192, 1363, 1606, 1752, 2122
Hpy8I	GTNNAC	4 cut(s)	173, 1280, 1282, 1979
Hpy99I	CGWCG	2 cut(s)	1196, 1442
HpyAV	CCTTC	4 cut(s)	440, 552, 590, 1436
HpyCH4III	ACNGT	6 cut(s)	55, 274, 612, 1286, 1968, 2135
HpyCH4IV	ACGT	6 cut(s)	292, 720, 1437, 1981, 2145, 2530
HpyF3I	CTNAG	9 cut(s)	321, 725, 1147, 1398, 1831, 1925, 1995, 2535, 2596
HpySE526I	ACGT	6 cut(s)	292, 720, 1437, 1981, 2145, 2530
Hsp92I	GRCGYC	1 cut(s)	1864
HspAI	GCGC	3 cut(s)	28, 1864, 2577
KasI	GGCGCC	1 cut(s)	1863
LguI	GCTCTTC	2 cut(s)	567, 1731
LmnI	GCTCC	3 cut(s)	41, 385, 1613
LweI	GCATC	8 cut(s)	679, 879, 1222, 1594, 2113, 2182, 2297, 2486
MaeI	CTAG	5 cut(s)	168, 521, 1803, 1827, 2285
MaeII	ACGT	6 cut(s)	292, 720, 1437, 1981, 2145, 2530
MaeIII	GTNAC	5 cut(s)	1223, 1322, 1636, 2129, 2135
MflI	RGATCY	3 cut(s)	1576, 1921, 2332
MhlI	GDGCHC	2 cut(s)	175, 408
MlsI	TGGCCA	1 cut(s)	2045
MluNI	TGGCCA	1 cut(s)	2045
Mly113I	GGCGCC	1 cut(s)	1864
MlyI	GAGTC	1 cut(s)	2588
MmeI	TCCRAC	4 cut(s)	478, 978, 1215, 1348
Mox20I	TGGCCA	1 cut(s)	2045
Mph1103I	ATGCAT	2 cut(s)	894, 2128
MroXI	GAANNNNTTC	2 cut(s)	2324, 2550
MscI	TGGCCA	1 cut(s)	2045
MslI	CAYNNNNRTG	1 cut(s)	912
Msp20I	TGGCCA	1 cut(s)	2045
MspA1I	CMGCKG	3 cut(s)	1151, 1308, 1397
MspCI	CTTAAG	2 cut(s)	314, 2344
MspI	CCGG	1 cut(s)	39
MspR9I	CCNGG	4 cut(s)	39, 1581, 1868, 2047
MvaI	CCWGG	3 cut(s)	1581, 1868, 2047
NarI	GGCGCC	1 cut(s)	1864
NciI	CCSGG	1 cut(s)	39
NcoI	CCATGG	2 cut(s)	1707, 2621
NheI	GCTAGC	1 cut(s)	1826
NlaIV	GGNNCC	6 cut(s)	569, 957, 1578, 1645, 1865, 2334
NmuCI	GTSAC	2 cut(s)	1322, 2129
NsiI	ATGCAT	2 cut(s)	894, 2128
NspI	RCATGY	3 cut(s)	368, 1957, 2130
PagI	TCATGA	2 cut(s)	913, 2215
PaqCI	CACCTGC	1 cut(s)	1537
PciSI	GCTCTTC	2 cut(s)	567, 1731
PdmI	GAANNNNTTC	2 cut(s)	2324, 2550
PflFI	GACNNNGTC	1 cut(s)	2597
PleI	GAGTC	1 cut(s)	2588
PluTI	GGCGCC	1 cut(s)	1867
PmaCI	CACGTG	1 cut(s)	1982
PmlI	CACGTG	1 cut(s)	1982
PpsI	GAGTC	1 cut(s)	2588
Ppu21I	YACGTR	1 cut(s)	1982
PpuMI	RGGWCCY	1 cut(s)	539
PshAI	GACNNNNGTC	1 cut(s)	2611
PshBI	ATTAAT	1 cut(s)	1017
PsiI	TTATAA	2 cut(s)	786, 1338
Psp5II	RGGWCCY	1 cut(s)	539
Psp6I	CCWGG	3 cut(s)	1579, 1866, 2045
PspCI	CACGTG	1 cut(s)	1982
PspEI	GGTNACC	1 cut(s)	1223
PspFI	CCCAGC	1 cut(s)	1035
PspGI	CCWGG	3 cut(s)	1579, 1866, 2045
PspN4I	GGNNCC	6 cut(s)	569, 957, 1578, 1645, 1865, 2334
PspPI	GGNCC	3 cut(s)	68, 539, 1644
PspPPI	RGGWCCY	1 cut(s)	539
PsrI	GAACNNNNNNTAC	2 cut(s)	627, 659
PstI	CTGCAG	2 cut(s)	940, 1782
PstNI	CAGNNNCTG	2 cut(s)	944, 1616
PsuI	RGATCY	3 cut(s)	1576, 1921, 2332
PsyI	GACNNNGTC	1 cut(s)	2597
PvuII	CAGCTG	2 cut(s)	1151, 1397
RsaI	GTAC	7 cut(s)	341, 586, 645, 750, 1281, 1544, 2362
RsaNI	GTAC	7 cut(s)	340, 585, 644, 749, 1280, 1543, 2361
RseI	CAYNNNNRTG	1 cut(s)	912
SapI	GCTCTTC	2 cut(s)	567, 1731
Sau96I	GGNCC	3 cut(s)	68, 539, 1644
ScaI	AGTACT	1 cut(s)	2362
SchI	GAGTC	1 cut(s)	2588
ScrFI	CCNGG	4 cut(s)	39, 1581, 1868, 2047
SduI	GDGCHC	2 cut(s)	175, 408
SfaNI	GCATC	8 cut(s)	679, 879, 1222, 1594, 2113, 2182, 2297, 2486
SfcI	CTRYAG	3 cut(s)	936, 1778, 2602
SfoI	GGCGCC	1 cut(s)	1865
SinI	GGWCC	2 cut(s)	539, 1644
SmiI	ATTTAAAT	1 cut(s)	2568
SmiMI	CAYNNNNRTG	1 cut(s)	912
SmlI	CTYRAG	6 cut(s)	314, 551, 1071, 1757, 2012, 2344
SmoI	CTYRAG	6 cut(s)	314, 551, 1071, 1757, 2012, 2344
SsiI	CCGC	9 cut(s)	34, 118, 301, 390, 823, 859, 1135, 1308, 1532
SspDI	GGCGCC	1 cut(s)	1863
SspI	AATATT	1 cut(s)	834
SspMI	CTAG	5 cut(s)	168, 521, 1803, 1827, 2285
StyD4I	CCNGG	4 cut(s)	37, 1579, 1866, 2045
StyI	CCWWGG	3 cut(s)	1707, 2487, 2621
SwaI	ATTTAAAT	1 cut(s)	2568
TaaI	ACNGT	6 cut(s)	55, 274, 612, 1286, 1968, 2135
TaiI	ACGT	6 cut(s)	295, 723, 1440, 1984, 2148, 2533
TaqI	TCGA	6 cut(s)	796, 922, 1142, 1159, 1440, 1719
TatI	WGTACW	2 cut(s)	1279, 2360
TauI	GCSGC	1 cut(s)	392
TscAI	CASTG	5 cut(s)	772, 1291, 1327, 1632, 1878
TseFI	GTSAC	2 cut(s)	1322, 2129
Tsp45I	GTSAC	2 cut(s)	1322, 2129
TspDTI	ATGAA	8 cut(s)	67, 669, 941, 1257, 1494, 2232, 2317, 2543
TspGWI	ACGGA	2 cut(s)	151, 2236
TspRI	CASTG	5 cut(s)	772, 1291, 1327, 1632, 1878
Tth111I	GACNNNGTC	1 cut(s)	2597
Vha464I	CTTAAG	2 cut(s)	314, 2344
VneI	GTGCAC	1 cut(s)	171
VpaK11BI	GGWCC	2 cut(s)	539, 1644
VspI	ATTAAT	1 cut(s)	1017
XapI	RAATTY	7 cut(s)	600, 671, 876, 1126, 1483, 2242, 2280
XceI	RCATGY	3 cut(s)	368, 1957, 2130
XmnI	GAANNNNTTC	2 cut(s)	2324, 2550
XspI	CTAG	5 cut(s)	168, 521, 1803, 1827, 2285
ZrmI	AGTACT	1 cut(s)	2362
Zsp2I	ATGCAT	2 cut(s)	894, 2128

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.