Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0006813

Physical Location & Seq

Genomic Coordinates Forward (+)

24719831 .. 24734004

Gene Structure 14174 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1184700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2634 bp

ATGTCCGCTTCTTTAGCTGCTTTTGAGCGCCCCCTTCGACCTGCTGCTTCTAACACGGTGTTCAAGAGTGGCCCACTTTTCATATCTTCTAAAGGAATAGGCTGGAAGTCTTGGAAGAAGCGGTGGTTTATCCTCACACGGACATCTTTGGTTTTCTTTAAAAATGATCCTAGTGCACTTCCACAAAGAGGTGGTGAAGTAAATTTGACTTTGGGGGGCATTGACTTGAACAATTCTGGGAGTGTTGTTGTAAGAGAAGATAAAAAGCTGCTGACCGTTTTATTTCCTGATGGACGTGATGGGCGGGCCTTCACTCTTAAGGCTGAGACATCAGAAGACCTGTATGAGTGGAAGACAGCCCTTGAACTTGCCCTTGCACAAGCTCCAAGTGCTGCCCTTGTCATGGGACACAATGGGATTTTCCGTAATGACACAAATGATACGCTTGAAGGGTCCTTCCATCAATGGAGGGATAAACGGCCTGTTAAATCTTTGGTTGTTGGAAGACCAATTCTGCTTGCGCTAGAAGATATTGATGGAGGTCCTTCCTTCCTCGAGAAAGCTCTTCGGTTCCTGGAAAAGTTTGGTACGAAAGCTGAAGGAATTTTGCGACAGTCTGCAGATGTTGAAGAGGTAGAACGTAGAGTACAAGATTATGAGCAAGGCAAGACTGAATTTGATCCGGACGAGGACGCTCATGTTGTTGGTGATTGTGTCAAGCATGTTCTGAGAGAACTGCCTTCATCTCCAGTACCAGCATCTTGCTGCACTGCATTGCTAGAGGCTTATAAAATTGATCGAAAGGAAGCTCGGATTAATGCAATACGCTCTTCAATATTGGAGACATTTCCCGAGCCAAACCGCCGTTTATTACAGAGAATTTTGAAGATGATGCATACCATATCTTCTCACTCTCATGAGAATCGGATGACTCCATCTGCAGTTGCTGCATGCATGGCACCCTTGCTCTTACGCCCTCTATTAGCTGGTGAATGTGAGCTGGATGATGACTTTGATATTAATGGTGATAATTCAACCCAACTTATTGCTGCTGCAAATGCTGCTAATAATGCTCAAGCTATCATCACAACTCTTTTGGAGGAGTATGAAAACGTTTTTCATGATGAAAATCTTCTAAGGTGTTCCATCTCTGCTGATTCTCGAATTGAGAATAGTGGGAGTGAAGATTCATCTGATGATGAAAATCTTGATCTGAAAGACAATGGTTACCATGACGCAGAAAATGAAGTTGATCTAGAAACAGATGATGATCCGGATAGAGTCCATAGTGGAAAGTTGAGTGAAAGCAGTGGTTATGCTGGCAGTGACCTCTATGATTATAAGGCGTTCGGGGTTGATGATTCAGATGTTGGATCACCTAAAGGAAATCATGCGTCAGCTCAGAGTTCAAGTTCATATGTTCCTCAACCCATCAGAGATCCAAATGTTGGAGTCGTGGAAAGAGGAAGCAAACCAAAAAAAGAAAATGAAAATTTAATTAGTGAGACAGAAACACCAAGTGCGTCACCTGCTGGAGATTCTTACAGATCAATGGGAGAGATTCTGTCAGTAGTGGATCCAGGGCATCATTTACTGGTTCCTGGACTTGATTCAGGAACTGCGAAGACAGCGGCTAAAGTTACAGGCTCCAATCATAATGCAAAGCGATCGGCATTTTGGGGAAGAAGCAATGCAAGAAAGACACCGTCAATGGAATCAGTTGATTCTTCTGGAGAGGAAGAGCTTTCAATTCAGAGGCTTGAGATTGCGAAAAACGACTTGCAGCACAGGATTGCGAAAGAGGCAAGAGGAAATGCAATATTACAAGCTAGTTTGGAGAGAAGGAAGCAAGCTTTACATGAGCGCCGCTTGGCACTGGAACAAGATGTTTCAAGATTGCAAGAGCAGCTACAAGCTGAACGAGATCTTAGAGCAGCATTAGAGGTTGGTTTGAGCATGTCTTCTGGACAGTTGGCCAGTTCACGTGGCATGGATTCTAAGACACGGGCTGAGCTTGAGGAGATAGCTCTTGCTGAAGCAGATGTGGCCAGGTTGAAGCAGAAAGTTGCAGAACTTCACCATCAACTTAATCAACAGCGACAGCATCATTATGGTTCTCTCTCTGATGCTTGTGACCGTTATCAAAATGGCCAAAATCATAATTCCCAACAGAGGTTTCTTCAGCAAGATTTTGATGCAACCCTTGCCTTCTGTAATCATGAAAGGAAACAAAGGAATGAGGAGAGTTTGCTGGGGGAAGATTGGAGGACTATCAAGGGCCAAATTTTAGCATCTGGCAGCAGTGTAAGGCAGCCTTCTAGGAAGCAATTCATGGATCCGGCTAGCATAAGTGAATCAAAAAGTACTGATGCATCGAAAAGTTTGTCTGTTGATGAGCTTTCTGCTGTTGATTCTGTGTCCATGCCATCAACTTCAAGAGCACCAGAGGTGGTGGATTATCCAAGGCATCCATCAACAGCATCTTCTGCCTTGGTAGAATTAACAACTCGTCTTGATTTCTTCAAGGAAAGACGTTCTCAGCTGATGGAACAGCTTCATAATCTTGATTTAAATTATGGCACGACATCTTTGCAAGACTCTGTTTATAGACCATCGTCCCCACCGTGGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0000919 GO:0003674 GO:0005096 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006996 GO:0007049 GO:0008047 GO:0008150 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009832 GO:0009920 GO:0009987 GO:0016020 GO:0016043 GO:0022402 GO:0022607 GO:0030234 GO:0030695 GO:0031976 GO:0031984 GO:0032506 GO:0034357 GO:0042546 GO:0042651 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043547 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0048285 GO:0050790 GO:0051301 GO:0051336 GO:0051345 GO:0055035 GO:0060589 GO:0061640 GO:0065007 GO:0065009 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0098772 GO:0140014 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lzipper-MIP1 PH RhoGAP

Protein Analysis

878

Amino Acids

96.98

Weight (kDa)

5.36

Isoelectric Point (pI)

52.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH	PF00169	21 - 125	1.6e-12	PH domain
RhoGAP	PF00620	182 - 326	3.5e-33	RhoGAP domain
Lzipper-MIP1	PF14389	619 - 699	1.9e-21	Leucine-zipper of ternary complex factor MIP1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006813)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19390 AT5G19390 AT5G19390 AT5G19390
fragaria_vesca	FvH4_3g27830 FvH4_3g27830 FvH4_3g27830 FvH4_3g27830
malus_domestica	MD03G1165400.v1.1 MD11G1184700.v1.1
prunus_persica	Prupe.4G265200_v2.0.a1 Prupe.4G265200_v2.0.a1 Prupe.4G265200_v2.0.a1 Prupe.4G265300_v2.0.a1
pyrus_communis	pycom03g11990 pycom12g08810
rosa_chinensis	RchiOBHm_Chr5g0050541
rosa_laevigata	RLG00000034705
rosa_multiflora	Rmu_sc0004301.1_g000009
rosa_roxburghii	Rroxscaffold_1G00030350
rosa_rugosa	Rorug05G0261400
rosa_samantha	Rh5AG333300 Rh5BG343200 Rh5CG370800 Rh5DG355900
rosa_wichuraiana	Rw5G031480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	789, 1341
AarI	CACCTGC	1 cut(s)	1537
Acc36I	ACCTGC	2 cut(s)	49, 1537
AccB1I	GGYRCC	1 cut(s)	958
AccB7I	CCANNNNNTGG	1 cut(s)	1448
AccIII	TCCGGA	2 cut(s)	682, 1273
AciI	CCGC	6 cut(s)	6, 121, 304, 862, 1631, 1867
AclI	AACGTT	1 cut(s)	1113
AclWI	GGATC	9 cut(s)	161, 674, 1265, 1381, 1433, 1571, 1584, 2330, 2343
AcoI	YGGCCR	3 cut(s)	1974, 2046, 2149
AcsI	RAATTY	6 cut(s)	202, 603, 674, 879, 1492, 2283
AcuI	CTGAAG	3 cut(s)	618, 2055, 2165
AcvI	CACGTG	1 cut(s)	1985
AdeI	CACNNNGTG	1 cut(s)	1520
AfaI	GTAC	4 cut(s)	589, 648, 753, 2365
AfiI	CCNNNNNNNGG	5 cut(s)	188, 403, 1448, 2172, 2625
AflII	CTTAAG	1 cut(s)	317
AjiI	CACGTC	1 cut(s)	296
AjnI	CCWGG	4 cut(s)	573, 1579, 1600, 2048
AjuI	GAANNNNNNNTTGG	2 cut(s)	2160, 2192
Alw21I	GWGCWC	2 cut(s)	178, 2443
Alw26I	GTCTC	3 cut(s)	320, 836, 1499
Alw44I	GTGCAC	1 cut(s)	174
AlwI	GGATC	9 cut(s)	161, 674, 1265, 1381, 1433, 1571, 1584, 2330, 2343
AlwNI	CAGNNNCTG	2 cut(s)	947, 1619
Ama87I	CYCGRG	2 cut(s)	554, 851
Aor13HI	TCCGGA	2 cut(s)	682, 1273
AoxI	GGCC	7 cut(s)	70, 306, 479, 1974, 2046, 2149, 2278
ApaLI	GTGCAC	1 cut(s)	174
ApoI	RAATTY	6 cut(s)	202, 603, 674, 879, 1492, 2283
ArsI	GACNNNNNNTTYG	2 cut(s)	2369, 2401
AseI	ATTAAT	2 cut(s)	816, 1020
Asp700I	GAANNNNTTC	3 cut(s)	1131, 2327, 2553
AspLEI	GCGC	3 cut(s)	30, 523, 1866
AspS9I	GGNCC	5 cut(s)	71, 306, 453, 542, 2278
AsuHPI	GGTGA	7 cut(s)	206, 719, 1001, 1037, 1368, 1518, 2069
AsuNHI	GCTAGC	1 cut(s)	2342
AvaI	CYCGRG	2 cut(s)	554, 851
AvaII	GGWCC	2 cut(s)	453, 542
BaeGI	GKGCMC	1 cut(s)	178
BalI	TGGCCA	3 cut(s)	1976, 2048, 2151
BamHI	GGATCC	2 cut(s)	1576, 2335
BanI	GGYRCC	1 cut(s)	958
BbrPI	CACGTG	1 cut(s)	1985
BbsI	GAAGAC	5 cut(s)	342, 359, 511, 1631, 1953
Bbv12I	GWGCWC	2 cut(s)	178, 2443
BceAI	ACGGC	2 cut(s)	494, 849
BcgI	CGANNNNNNTGC	4 cut(s)	1650, 1684, 2572, 2606
BciT130I	CCWGG	4 cut(s)	575, 1581, 1602, 2050
BcoDI	GTCTC	3 cut(s)	320, 836, 1499
BfaI	CTAG	7 cut(s)	171, 524, 779, 1256, 1830, 2319, 2343
BfmI	CTRYAG	2 cut(s)	618, 939
BfoI	RGCGCY	2 cut(s)	31, 1867
BfrI	CTTAAG	1 cut(s)	317
BfuAI	ACCTGC	2 cut(s)	49, 1537
BglII	AGATCT	1 cut(s)	1924
BlpI	GCTNAGC	1 cut(s)	2010
BmcAI	AGTACT	1 cut(s)	2365
Bme1390I	CCNGG	4 cut(s)	575, 1581, 1602, 2050
Bme18I	GGWCC	2 cut(s)	453, 542
BmeT110I	CYCGRG	2 cut(s)	554, 851
BmgBI	CACGTC	1 cut(s)	296
BmgT120I	GGNCC	5 cut(s)	71, 306, 453, 542, 2278
BmiI	GGNNCC	7 cut(s)	454, 572, 960, 1578, 1599, 1648, 2337
BmrFI	CCNGG	4 cut(s)	575, 1581, 1602, 2050
BmtI	GCTAGC	1 cut(s)	2346
BoxI	GACNNNNGTC	1 cut(s)	2614
BpiI	GAAGAC	5 cut(s)	342, 359, 511, 1631, 1953
BpmI	CTGGAG	3 cut(s)	732, 1554, 1752
Bpu1102I	GCTNAGC	1 cut(s)	2010
BpuEI	CTTGAG	3 cut(s)	1059, 1781, 2036
BsaAI	YACGTR	1 cut(s)	1985
BsaBI	GATNNNNATC	3 cut(s)	1128, 1203, 1269
BsaJI	CCNNGG	4 cut(s)	1580, 2462, 2490, 2624
BsaWI	WCCGGW	2 cut(s)	682, 1273
Bsc4I	CCNNNNNNNGG	5 cut(s)	188, 403, 1448, 2172, 2625
Bse1I	ACTGG	4 cut(s)	749, 1599, 1881, 1977
Bse3DI	GCAATG	2 cut(s)	773, 1696
Bse8I	GATNNNNATC	3 cut(s)	1128, 1203, 1269
BseAI	TCCGGA	2 cut(s)	682, 1273
BseBI	CCWGG	4 cut(s)	575, 1581, 1602, 2050
BseDI	CCNNGG	4 cut(s)	1580, 2462, 2490, 2624
BseGI	GGATG	3 cut(s)	933, 1009, 2467
BseJI	GATNNNNATC	3 cut(s)	1128, 1203, 1269
BseLI	CCNNNNNNNGG	5 cut(s)	188, 403, 1448, 2172, 2625
BseMI	GCAATG	2 cut(s)	773, 1696
BseMII	CTCAG	5 cut(s)	315, 719, 1415, 2001, 2552
BseNI	ACTGG	4 cut(s)	749, 1599, 1881, 1977
BseRI	GAGGAG	3 cut(s)	1115, 2033, 2255
BseSI	GKGCMC	1 cut(s)	178
BseYI	CCCAGC	1 cut(s)	2251
BsgI	GTGCAG	1 cut(s)	751
Bsh1285I	CGRYCG	1 cut(s)	1670
BshFI	GGCC	7 cut(s)	72, 308, 481, 1976, 2048, 2151, 2280
BshNI	GGYRCC	1 cut(s)	958
BsiEI	CGRYCG	1 cut(s)	1670
BsiHKAI	GWGCWC	2 cut(s)	178, 2443
BsiHKCI	CYCGRG	2 cut(s)	554, 851
BsiSI	CCGG	3 cut(s)	683, 1274, 2339
BslFI	GGGAC	2 cut(s)	420, 2602
BslI	CCNNNNNNNGG	5 cut(s)	188, 403, 1448, 2172, 2625
BsmAI	GTCTC	3 cut(s)	320, 836, 1499
BsmFI	GGGAC	2 cut(s)	420, 2602
BsnI	GGCC	7 cut(s)	72, 308, 481, 1976, 2048, 2151, 2280
BsoBI	CYCGRG	2 cut(s)	554, 851
Bsp1286I	GDGCHC	2 cut(s)	178, 2443
Bsp13I	TCCGGA	2 cut(s)	682, 1273
Bsp1720I	GCTNAGC	1 cut(s)	2010
BspACI	CCGC	6 cut(s)	6, 121, 304, 862, 1631, 1867
BspANI	GGCC	7 cut(s)	72, 308, 481, 1976, 2048, 2151, 2280
BspCNI	CTCAG	5 cut(s)	316, 720, 1414, 2002, 2551
BspEI	TCCGGA	2 cut(s)	682, 1273
BspHI	TCATGA	3 cut(s)	916, 1120, 2218
BspLI	GGNNCC	7 cut(s)	454, 572, 960, 1578, 1599, 1648, 2337
BspMAI	CTGCAG	2 cut(s)	622, 943
BspMI	ACCTGC	2 cut(s)	49, 1537
BspOI	GCTAGC	1 cut(s)	2346
BspPI	GGATC	9 cut(s)	161, 674, 1265, 1381, 1433, 1571, 1584, 2330, 2343
BspQI	GCTCTTC	3 cut(s)	570, 835, 1734
BspT107I	GGYRCC	1 cut(s)	958
BspTI	CTTAAG	1 cut(s)	317
BsrDI	GCAATG	2 cut(s)	773, 1696
BsrI	ACTGG	4 cut(s)	749, 1599, 1881, 1977
BssECI	CCNNGG	4 cut(s)	1580, 2462, 2490, 2624
BssT1I	CCWWGG	2 cut(s)	2462, 2490
Bst2UI	CCWGG	4 cut(s)	575, 1581, 1602, 2050
Bst4CI	ACNGT	7 cut(s)	58, 277, 615, 1707, 1971, 2138, 2625
Bst6I	CTCTTC	4 cut(s)	570, 624, 835, 1734
BstAFI	CTTAAG	1 cut(s)	317
BstAPI	GCANNNNNTGC	4 cut(s)	947, 1061, 2204, 2486
BstBAI	YACGTR	1 cut(s)	1985
BstC8I	GCNNGC	6 cut(s)	306, 519, 952, 1321, 1851, 2344
BstDEI	CTNAG	8 cut(s)	324, 728, 1136, 1401, 1928, 1998, 2010, 2538
BstDSI	CCRYGG	1 cut(s)	2624
BstEII	GGTNACC	1 cut(s)	1226
BstF5I	GGATG	3 cut(s)	933, 1009, 2467
BstH2I	RGCGCY	2 cut(s)	31, 1867
BstHHI	GCGC	3 cut(s)	30, 523, 1866
BstMAI	GTCTC	3 cut(s)	320, 836, 1499
BstMCI	CGRYCG	1 cut(s)	1670
BstNI	CCWGG	4 cut(s)	575, 1581, 1602, 2050
BstNSI	RCATGY	3 cut(s)	725, 954, 1960
BstPAI	GACNNNNGTC	1 cut(s)	2614
BstPI	GGTNACC	1 cut(s)	1226
BstSCI	CCNGG	4 cut(s)	573, 1579, 1600, 2048
BstSFI	CTRYAG	2 cut(s)	618, 939
BstSLI	GKGCMC	1 cut(s)	178
BstV2I	GAAGAC	5 cut(s)	342, 359, 511, 1631, 1953
BstX2I	RGATCY	4 cut(s)	1438, 1576, 1924, 2335
BstYI	RGATCY	4 cut(s)	1438, 1576, 1924, 2335
BsuRI	GGCC	7 cut(s)	72, 308, 481, 1976, 2048, 2151, 2280
BtgI	CCRYGG	1 cut(s)	2624
BtrI	CACGTC	1 cut(s)	296
BtsCI	GGATG	3 cut(s)	933, 1009, 2467
BtsI	GCAGTG	4 cut(s)	768, 1315, 1330, 2308
BtsIMutI	CAGTG	5 cut(s)	768, 1315, 1330, 1874, 2308
BveI	ACCTGC	2 cut(s)	49, 1537
Cac8I	GCNNGC	6 cut(s)	306, 519, 952, 1321, 1851, 2344
CaiI	CAGNNNCTG	2 cut(s)	947, 1619
CciI	TCATGA	3 cut(s)	916, 1120, 2218
CfoI	GCGC	3 cut(s)	30, 523, 1866
Cfr13I	GGNCC	5 cut(s)	71, 306, 453, 542, 2278
CseI	GACGC	4 cut(s)	701, 1244, 1383, 1512
Csp6I	GTAC	4 cut(s)	588, 647, 752, 2364
CviQI	GTAC	4 cut(s)	588, 647, 752, 2364
DdeI	CTNAG	8 cut(s)	324, 728, 1136, 1401, 1928, 1998, 2010, 2538
DraI	TTTAAA	2 cut(s)	160, 2571
DraIII	CACNNNGTG	1 cut(s)	1520
EaeI	YGGCCR	3 cut(s)	1974, 2046, 2149
Eam1104I	CTCTTC	4 cut(s)	570, 624, 835, 1734
EarI	CTCTTC	4 cut(s)	570, 624, 835, 1734
Eco130I	CCWWGG	2 cut(s)	2462, 2490
Eco47I	GGWCC	2 cut(s)	453, 542
Eco57I	CTGAAG	3 cut(s)	618, 2055, 2165
Eco72I	CACGTG	1 cut(s)	1985
Eco88I	CYCGRG	2 cut(s)	554, 851
Eco91I	GGTNACC	1 cut(s)	1226
EcoO109I	RGGNCCY	2 cut(s)	453, 542
EcoO65I	GGTNACC	1 cut(s)	1226
EcoRII	CCWGG	4 cut(s)	573, 1579, 1600, 2048
EcoT14I	CCWWGG	2 cut(s)	2462, 2490
EcoT22I	ATGCAT	3 cut(s)	897, 956, 2374
ErhI	CCWWGG	2 cut(s)	2462, 2490
FalI	AAGNNNNNCTT	2 cut(s)	2299, 2331
FaqI	GGGAC	2 cut(s)	420, 2602
FauI	CCCGC	1 cut(s)	297
FauNDI	CATATG	1 cut(s)	1417
FokI	GGATG	3 cut(s)	940, 1016, 2454
FspBI	CTAG	7 cut(s)	171, 524, 779, 1256, 1830, 2319, 2343
GlaI	GCGC	3 cut(s)	29, 522, 1865
GsaI	CCCAGC	1 cut(s)	2255
GsuI	CTGGAG	3 cut(s)	732, 1554, 1752
HaeII	RGCGCY	2 cut(s)	31, 1867
HaeIII	GGCC	7 cut(s)	72, 308, 481, 1976, 2048, 2151, 2280
HapII	CCGG	3 cut(s)	683, 1274, 2339
HgaI	GACGC	4 cut(s)	701, 1244, 1383, 1512
HhaI	GCGC	3 cut(s)	30, 523, 1866
Hin6I	GCGC	3 cut(s)	28, 521, 1864
HinP1I	GCGC	3 cut(s)	28, 521, 1864
HindIII	AAGCTT	1 cut(s)	1851
HpaII	CCGG	3 cut(s)	683, 1274, 2339
HphI	GGTGA	7 cut(s)	206, 719, 1001, 1037, 1368, 1518, 2069
Hpy166II	GTNNAC	2 cut(s)	176, 1982
Hpy8I	GTNNAC	2 cut(s)	176, 1982
HpyCH4III	ACNGT	7 cut(s)	58, 277, 615, 1707, 1971, 2138, 2625
HpyCH4IV	ACGT	5 cut(s)	295, 640, 1113, 1984, 2533
HpyF3I	CTNAG	8 cut(s)	324, 728, 1136, 1401, 1928, 1998, 2010, 2538
HpySE526I	ACGT	5 cut(s)	295, 640, 1113, 1984, 2533
HspAI	GCGC	3 cut(s)	28, 521, 1864
Kpn2I	TCCGGA	2 cut(s)	682, 1273
LguI	GCTCTTC	3 cut(s)	570, 835, 1734
LmnI	GCTCC	2 cut(s)	388, 1652
MaeI	CTAG	7 cut(s)	171, 524, 779, 1256, 1830, 2319, 2343
MaeII	ACGT	5 cut(s)	295, 640, 1113, 1984, 2533
MaeIII	GTNAC	5 cut(s)	1226, 1325, 1524, 1639, 2132
MflI	RGATCY	4 cut(s)	1438, 1576, 1924, 2335
MhlI	GDGCHC	2 cut(s)	178, 2443
MlsI	TGGCCA	3 cut(s)	1976, 2048, 2151
MluNI	TGGCCA	3 cut(s)	1976, 2048, 2151
MlyI	GAGTC	4 cut(s)	925, 1290, 1461, 2591
MmeI	TCCRAC	3 cut(s)	481, 1351, 1429
Mox20I	TGGCCA	3 cut(s)	1976, 2048, 2151
Mph1103I	ATGCAT	3 cut(s)	897, 956, 2374
MroI	TCCGGA	2 cut(s)	682, 1273
MroXI	GAANNNNTTC	3 cut(s)	1131, 2327, 2553
MscI	TGGCCA	3 cut(s)	1976, 2048, 2151
MseI	TTAA	9 cut(s)	159, 318, 486, 816, 1020, 1496, 2088, 2501, 2570
MslI	CAYNNNNRTG	2 cut(s)	915, 2109
Msp20I	TGGCCA	3 cut(s)	1976, 2048, 2151
MspA1I	CMGCKG	2 cut(s)	1631, 2542
MspCI	CTTAAG	1 cut(s)	317
MspI	CCGG	3 cut(s)	683, 1274, 2339
MspR9I	CCNGG	4 cut(s)	575, 1581, 1602, 2050
MvaI	CCWGG	4 cut(s)	575, 1581, 1602, 2050
NdeI	CATATG	1 cut(s)	1417
NheI	GCTAGC	1 cut(s)	2342
NlaIV	GGNNCC	7 cut(s)	454, 572, 960, 1578, 1599, 1648, 2337
NmuCI	GTSAC	3 cut(s)	1325, 1524, 2132
NsiI	ATGCAT	3 cut(s)	897, 956, 2374
NspI	RCATGY	3 cut(s)	725, 954, 1960
PaeI	GCATGC	1 cut(s)	954
PaeR7I	CTCGAG	1 cut(s)	554
PagI	TCATGA	3 cut(s)	916, 1120, 2218
PaqCI	CACCTGC	1 cut(s)	1537
PciSI	GCTCTTC	3 cut(s)	570, 835, 1734
PcsI	WCGNNNNNNNCGW	1 cut(s)	2621
PdmI	GAANNNNTTC	3 cut(s)	1131, 2327, 2553
PflFI	GACNNNGTC	1 cut(s)	1705
PflMI	CCANNNNNTGG	1 cut(s)	1448
PfoI	TCCNGGA	2 cut(s)	573, 1600
Ple19I	CGATCG	1 cut(s)	1670
PleI	GAGTC	4 cut(s)	925, 1289, 1460, 2591
PmaCI	CACGTG	1 cut(s)	1985
PmlI	CACGTG	1 cut(s)	1985
PpsI	GAGTC	4 cut(s)	925, 1289, 1460, 2591
Ppu21I	YACGTR	1 cut(s)	1985
PpuMI	RGGWCCY	2 cut(s)	453, 542
PshAI	GACNNNNGTC	1 cut(s)	2614
PshBI	ATTAAT	2 cut(s)	816, 1020
PsiI	TTATAA	2 cut(s)	789, 1341
Psp1406I	AACGTT	1 cut(s)	1113
Psp5II	RGGWCCY	2 cut(s)	453, 542
Psp6I	CCWGG	4 cut(s)	573, 1579, 1600, 2048
PspCI	CACGTG	1 cut(s)	1985
PspEI	GGTNACC	1 cut(s)	1226
PspFI	CCCAGC	1 cut(s)	2251
PspGI	CCWGG	4 cut(s)	573, 1579, 1600, 2048
PspN4I	GGNNCC	7 cut(s)	454, 572, 960, 1578, 1599, 1648, 2337
PspPI	GGNCC	5 cut(s)	71, 306, 453, 542, 2278
PspPPI	RGGWCCY	2 cut(s)	453, 542
PsrI	GAACNNNNNNTAC	2 cut(s)	630, 662
PstI	CTGCAG	2 cut(s)	622, 943
PstNI	CAGNNNCTG	2 cut(s)	947, 1619
PsuI	RGATCY	4 cut(s)	1438, 1576, 1924, 2335
PsyI	GACNNNGTC	1 cut(s)	1705
PvuI	CGATCG	1 cut(s)	1670
PvuII	CAGCTG	1 cut(s)	2542
RsaI	GTAC	4 cut(s)	589, 648, 753, 2365
RsaNI	GTAC	4 cut(s)	588, 647, 752, 2364
RseI	CAYNNNNRTG	2 cut(s)	915, 2109
SapI	GCTCTTC	3 cut(s)	570, 835, 1734
SaqAI	TTAA	9 cut(s)	159, 318, 486, 816, 1020, 1496, 2088, 2501, 2570
Sau96I	GGNCC	5 cut(s)	71, 306, 453, 542, 2278
ScaI	AGTACT	1 cut(s)	2365
SchI	GAGTC	4 cut(s)	925, 1290, 1461, 2591
ScrFI	CCNGG	4 cut(s)	575, 1581, 1602, 2050
SduI	GDGCHC	2 cut(s)	178, 2443
SfcI	CTRYAG	2 cut(s)	618, 939
Sfr274I	CTCGAG	1 cut(s)	554
SinI	GGWCC	2 cut(s)	453, 542
SlaI	CTCGAG	1 cut(s)	554
SmiI	ATTTAAAT	1 cut(s)	2571
SmiMI	CAYNNNNRTG	2 cut(s)	915, 2109
SmlI	CTYRAG	5 cut(s)	317, 554, 1074, 1760, 2015
SmoI	CTYRAG	5 cut(s)	317, 554, 1074, 1760, 2015
SphI	GCATGC	1 cut(s)	954
SsiI	CCGC	6 cut(s)	6, 121, 304, 862, 1631, 1867
SspI	AATATT	2 cut(s)	837, 1821
SspMI	CTAG	7 cut(s)	171, 524, 779, 1256, 1830, 2319, 2343
StyD4I	CCNGG	4 cut(s)	573, 1579, 1600, 2048
StyI	CCWWGG	2 cut(s)	2462, 2490
SwaI	ATTTAAAT	1 cut(s)	2571
TaaI	ACNGT	7 cut(s)	58, 277, 615, 1707, 1971, 2138, 2625
TaiI	ACGT	5 cut(s)	298, 643, 1116, 1987, 2536
TaqI	TCGA	5 cut(s)	37, 555, 799, 1162, 2375
TatI	WGTACW	2 cut(s)	646, 2363
TauI	GCSGC	2 cut(s)	1634, 1869
Tru1I	TTAA	9 cut(s)	159, 318, 486, 816, 1020, 1496, 2088, 2501, 2570
Tru9I	TTAA	9 cut(s)	159, 318, 486, 816, 1020, 1496, 2088, 2501, 2570
TscAI	CASTG	5 cut(s)	775, 1315, 1330, 1881, 2308
TseFI	GTSAC	3 cut(s)	1325, 1524, 2132
Tsp45I	GTSAC	3 cut(s)	1325, 1524, 2132
TspGWI	ACGGA	2 cut(s)	154, 413
TspRI	CASTG	5 cut(s)	775, 1315, 1330, 1881, 2308
Tth111I	GACNNNGTC	1 cut(s)	1705
Van91I	CCANNNNNTGG	1 cut(s)	1448
Vha464I	CTTAAG	1 cut(s)	317
VneI	GTGCAC	1 cut(s)	174
VpaK11BI	GGWCC	2 cut(s)	453, 542
VspI	ATTAAT	2 cut(s)	816, 1020
XapI	RAATTY	6 cut(s)	202, 603, 674, 879, 1492, 2283
XbaI	TCTAGA	1 cut(s)	1255
XceI	RCATGY	3 cut(s)	725, 954, 1960
XhoI	CTCGAG	1 cut(s)	554
XmnI	GAANNNNTTC	3 cut(s)	1131, 2327, 2553
XspI	CTAG	7 cut(s)	171, 524, 779, 1256, 1830, 2319, 2343
ZrmI	AGTACT	1 cut(s)	2365
Zsp2I	ATGCAT	3 cut(s)	897, 956, 2374

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.