Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003512

Physical Location & Seq

Genomic Coordinates Forward (+)

26234898 .. 26241245

Gene Structure 6348 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1190800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2091 bp

ATGTCAAGTTATTTGGGTGTTCACATTATGGATCAATCGCTTCAAAGCCAGTTCACGCAGGTTGAGCTTCGCAGTCTAGAATCCAAATTTAATTCAGTGAAGAATCAGAATGGTAAAGTTTTGGCTGGAGATTTGCCACCTTTAATGATGAAATTAAAGGCATTTAGAGAAATGTATTCTGAGGAGGAGATCAGAGGTATCTTAAGCGGGTTGGAGTCTGACTTCAGTGACGAGATTGATTTTGAATCCTTCCTTAAGGCATACTTAATTTTGCAAGGGCAAACAACGGCAAAATCGGGTGGTTCAAAGAACTCTTCATCATTCTTGAAGGCCACTACAACCACCCTCCTTCATACAATCAGTGAATCAGAGAGAGCATCTTATGTGGCTCACATAAACAGCTATCTTGGGGATGACCCATTTCTAAAGCAGTTTCTCCCATTAGACCCATCTACAAATGACCTATTCCACCTTGCAAAAGATGGAGTTCTCCTCTGCAAGCTTATAAATGTTGCTGTGCCTGGGACAATAGATGAACGAGCAATTAACACCAAAAGGGTTCTCAACCCATGGGAGAGGAATGAGAACCATACTCTTTGCCTCAACTCTGCCAAGGCCATTGGCTGCACAGTGGTTAACATTGGCACACAGGACCTGGTTGAAGGCAGACCTCATTTGGTATTGGGGTTGATTTCGCAAATTATAAAGATCCAACTGTTGGCAGATCTCAATCTTAAGAAGACACCTCAGCTTGTAGAATTGGTGGATGATAGCAAGGATGTTGAAGAGCTTTTGAGTTTACCCCCTGAGAAGGTCTTGTTGAAATGGATGAATTTTCACCTCCAGAAAGCAGGCTACAAGAAACCTGTTTCAAACTTTTCTTCTGACGTGAAGGATGGAGAAGCTTATGCTTACCTACTTAATGTTCTTGCTCCAGAACACTGCAATCCCGCTACATTGGATGCCAAACCCAATGAAAGGGCAAAGTTGGTTCTCGATCATGCTGAGAGAATGAACTGCAAAAGATACTTGACTCCAAAAGACATTCTCGAGGGTTCTTCAAACTTAAATCTGGCATTTGTGGCACAAATATTCCATGAAAGGAATGGCCTCTCTACAGACAGCAAGAAGATTTCTTTTGCAGAGATGATGACAGATGATGTGCAAACATCAAGAGAAGAGAGATGTTTCCGGCTGTGGATCAACAGTCTAGGCATTGGTAGTTATGTTAATAATGTGTTTGAAGATGTGAGAAATGGATGGATACTATTAGAAGTACTCGATAAGGTTTCACCAGGTTCAGTTAACTGGAAGCAAGCATCAAAGCCTCCAATTAAAATGCCGTTTAGAAAAGTGGAGAATTGCAATCAAGTTGTAAGGATTGGAAAGCAACTGAAATTCTCGCTGGTGAATGTAGCTGGAAATGATATTGTGCAGGGAAACAAGAAACTCATACTTGCATTCCTATGGCAGTTGATGAGGTTTAATATGCTTCAACTTTTGAAAAATCTAAGATCTCACTCCCAAGGAAAGGAGATGACAGACGCTGATATTTTAAAGTGGGCTAACAACAAAGTCAGAAGCACAGGCAGAACTTCTCAAATGGAGAGTTTTAAGGATAAGAGCCTGTCAAACGGGATTTTCTTCCTCGAGCTTCTCAGTGCTGTGGAGCCTAGAGTTGTCAATTGGAATCTTGTTACCAAGGGTGAAAGTGCTGATGAAAAGAAGTTGAATGCTACATACATAGTCAGCGTTGCACGGAAGCTTGGCTGTTCCATATTCTTGTTGCCTGAGGACATCATGGAGGTGAATCAAAAGATGCTTTTGACACTAACAGCCAGCATTATGTTTTGGAGTCTGCAACAACCGGTTGATGACACAGAAAGATTGCCATCCCCTGTTGACGCGTCACCGGCAACATCTATCAGCGGAGAGGATGAAAGCTGTTCTTCTCTTGGCGGTGAAATTTCAAACTTGAGCATCGACGACACTGCCTCAGACACTACACAGGTTGAGAATGATGGCAGCAACACTCCTCTGGGAGGCGGGATCGGGGTGGAAGGGGAAGGAGGGTGCATAGCTGTTAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005856 GO:0005884 GO:0006996 GO:0007010 GO:0007015 GO:0007623 GO:0008092 GO:0008150 GO:0009987 GO:0015629 GO:0016043 GO:0022607 GO:0030029 GO:0030036 GO:0032432 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0048511 GO:0051015 GO:0051017 GO:0051639 GO:0051764 GO:0061572 GO:0071840 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Protein Analysis

697

Amino Acids

77.28

Weight (kDa)

5.74

Isoelectric Point (pI)

35.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	151 - 236	1.3e-16	Calponin homology (CH) domain
CH	PF00307	268 - 367	1.8e-22	Calponin homology (CH) domain
CH	PF00307	393 - 495	4.9e-18	Calponin homology (CH) domain
CH	PF00307	515 - 616	8.5e-18	Calponin homology (CH) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003512)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26700 AT4G26700 AT4G26700 AT4G26700 AT4G26700 AT4G26700 AT4G26700 AT5G55400 AT5G55400 AT5G55400
fragaria_vesca	FvH4_3g23730 FvH4_3g23730 FvH4_3g23730 FvH4_3g23730 FvH4_3g23730 FvH4_3g23730
malus_domestica	MD03G1190800.v1.1 MD04G1101600.v1.1 MD11G1207500.v1.1
prunus_persica	Prupe.4G227900_v2.0.a1
pyrus_communis	pycom03g14400 pycom03g14410 pycom11g18070
rosa_chinensis	RchiOBHm_Chr5g0041891
rosa_laevigata	RLG00000034102 RLG00000034103
rosa_multiflora	Rmu_sc0009443.1_g000014
rosa_roxburghii	Rroxscaffold_1G00038650
rosa_rugosa	Rorug05G0196900 Rorug05G0197000
rosa_samantha	Rh5AG281500 Rh5BG287100 Rh5CG318700 Rh5DG295800
rosa_wichuraiana	Rw0G001340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	506, 704
Acc36I	ACCTGC	1 cut(s)	49
AccB7I	CCANNNNNTGG	1 cut(s)	718
AccII	CGCG	1 cut(s)	1907
AciI	CCGC	5 cut(s)	207, 951, 1929, 1959, 2046
AclWI	GGATC	4 cut(s)	39, 703, 1208, 2057
AcsI	RAATTY	4 cut(s)	86, 832, 1397, 1965
AcuI	CTGAAG	1 cut(s)	208
AfaI	GTAC	1 cut(s)	1278
AfiI	CCNNNNNNNGG	5 cut(s)	718, 811, 978, 1531, 2042
AflII	CTTAAG	3 cut(s)	202, 254, 734
AflIII	ACRYGT	1 cut(s)	1905
AgeI	ACCGGT	1 cut(s)	1867
AjiI	CACGTC	1 cut(s)	889
AjnI	CCWGG	3 cut(s)	520, 654, 1294
AlwI	GGATC	4 cut(s)	39, 703, 1208, 2057
AlwNI	CAGNNNCTG	2 cut(s)	655, 1547
Ama87I	CYCGRG	2 cut(s)	1049, 1649
AoxI	GGCC	3 cut(s)	330, 615, 1108
ApeKI	GCWGC	2 cut(s)	624, 2025
ApoI	RAATTY	4 cut(s)	86, 832, 1397, 1965
AsiGI	ACCGGT	1 cut(s)	1867
AspS9I	GGNCC	1 cut(s)	652
AsuHPI	GGTGA	7 cut(s)	830, 1284, 1420, 1718, 1819, 1902, 1973
AvaI	CYCGRG	2 cut(s)	1049, 1649
AvaII	GGWCC	1 cut(s)	652
AxyI	CCTNAGG	1 cut(s)	1791
BbsI	GAAGAC	1 cut(s)	746
BbvCI	CCTCAGC	1 cut(s)	747
BbvI	GCAGC	2 cut(s)	611, 2037
BccI	CCATC	6 cut(s)	457, 476, 890, 1254, 1900, 2015
BceAI	ACGGC	2 cut(s)	303, 1327
BcgI	CGANNNNNNTGC	2 cut(s)	1973, 2007
BciT130I	CCWGG	3 cut(s)	522, 656, 1296
BciVI	GTATCC	1 cut(s)	1257
BfaI	CTAG	3 cut(s)	77, 1211, 1674
BfmI	CTRYAG	1 cut(s)	1116
BfrI	CTTAAG	3 cut(s)	202, 254, 734
BfuAI	ACCTGC	1 cut(s)	49
BfuI	GTATCC	1 cut(s)	1257
BglII	AGATCT	2 cut(s)	724, 1514
BisI	GCNGC	2 cut(s)	625, 2026
BlsI	GCNGC	2 cut(s)	626, 2027
BmcAI	AGTACT	1 cut(s)	1278
Bme1390I	CCNGG	3 cut(s)	522, 656, 1296
Bme18I	GGWCC	1 cut(s)	652
BmeT110I	CYCGRG	2 cut(s)	1049, 1649
BmgBI	CACGTC	1 cut(s)	889
BmgT120I	GGNCC	1 cut(s)	652
BmiI	GGNNCC	1 cut(s)	1671
BmrFI	CCNGG	3 cut(s)	522, 656, 1296
BmsI	GCATC	5 cut(s)	386, 952, 1328, 1809, 1989
BpiI	GAAGAC	1 cut(s)	746
BplI	GAGNNNNNCTC	2 cut(s)	477, 509
BpmI	CTGGAG	3 cut(s)	147, 827, 918
Bpu10I	CCTNAGC	1 cut(s)	747
BpuEI	CTTGAG	1 cut(s)	1996
BsaBI	GATNNNNATC	2 cut(s)	729, 1891
BsaJI	CCNNGG	5 cut(s)	521, 569, 612, 1525, 1701
BsaWI	WCCGGW	1 cut(s)	1867
Bsc4I	CCNNNNNNNGG	5 cut(s)	718, 811, 978, 1531, 2042
Bse118I	RCCGGY	2 cut(s)	1867, 1912
Bse1I	ACTGG	2 cut(s)	49, 1313
Bse21I	CCTNAGG	1 cut(s)	1791
Bse8I	GATNNNNATC	2 cut(s)	729, 1891
BseBI	CCWGG	3 cut(s)	522, 656, 1296
BseDI	CCNNGG	5 cut(s)	521, 569, 612, 1525, 1701
BseGI	GGATG	9 cut(s)	418, 772, 784, 834, 901, 967, 1265, 1892, 1942
BseJI	GATNNNNATC	2 cut(s)	729, 1891
BseLI	CCNNNNNNNGG	5 cut(s)	718, 811, 978, 1531, 2042
BseMII	CTCAG	7 cut(s)	171, 761, 798, 996, 1672, 1782, 2010
BseNI	ACTGG	2 cut(s)	49, 1313
BseRI	GAGGAG	4 cut(s)	197, 200, 482, 2025
BseXI	GCAGC	2 cut(s)	611, 2037
BsgI	GTGCAG	2 cut(s)	610, 1454
Bsh1236I	CGCG	1 cut(s)	1907
BshFI	GGCC	3 cut(s)	332, 617, 1110
BshTI	ACCGGT	1 cut(s)	1867
BsiHKCI	CYCGRG	2 cut(s)	1049, 1649
BsiSI	CCGG	3 cut(s)	1192, 1868, 1913
BslFI	GGGAC	1 cut(s)	538
BslI	CCNNNNNNNGG	5 cut(s)	718, 811, 978, 1531, 2042
BsmFI	GGGAC	1 cut(s)	538
BsmI	GAATGC	2 cut(s)	1460, 1738
BsnI	GGCC	3 cut(s)	332, 617, 1110
BsoBI	CYCGRG	2 cut(s)	1049, 1649
Bsp143I	GATC	8 cut(s)	31, 189, 708, 724, 997, 1200, 1514, 2049
Bsp19I	CCATGG	1 cut(s)	569
BspACI	CCGC	5 cut(s)	207, 951, 1929, 1959, 2046
BspANI	GGCC	3 cut(s)	332, 617, 1110
BspCNI	CTCAG	7 cut(s)	172, 760, 799, 997, 1671, 1783, 2009
BspFNI	CGCG	1 cut(s)	1907
BspLI	GGNNCC	1 cut(s)	1671
BspMI	ACCTGC	1 cut(s)	49
BspPI	GGATC	4 cut(s)	39, 703, 1208, 2057
BspQI	GCTCTTC	1 cut(s)	780
BspTI	CTTAAG	3 cut(s)	202, 254, 734
BsrFI	RCCGGY	2 cut(s)	1867, 1912
BsrI	ACTGG	2 cut(s)	49, 1313
BssAI	RCCGGY	2 cut(s)	1867, 1912
BssECI	CCNNGG	5 cut(s)	521, 569, 612, 1525, 1701
BssMI	GATC	8 cut(s)	31, 189, 708, 724, 997, 1200, 1514, 2049
BssT1I	CCWWGG	4 cut(s)	569, 612, 1525, 1701
Bst2UI	CCWGG	3 cut(s)	522, 656, 1296
Bst4CI	ACNGT	3 cut(s)	631, 717, 1208
Bst6I	CTCTTC	3 cut(s)	319, 780, 1173
BstAFI	CTTAAG	3 cut(s)	202, 254, 734
BstC8I	GCNNGC	4 cut(s)	500, 853, 1317, 1840
BstDEI	CTNAG	8 cut(s)	180, 747, 807, 1005, 1511, 1658, 1791, 1996
BstDSI	CCRYGG	1 cut(s)	569
BstENI	CCTNNNNNAGG	1 cut(s)	2040
BstF5I	GGATG	9 cut(s)	418, 772, 784, 834, 901, 967, 1265, 1892, 1942
BstFNI	CGCG	1 cut(s)	1907
BstKTI	GATC	8 cut(s)	34, 192, 711, 727, 1000, 1203, 1517, 2052
BstMBI	GATC	8 cut(s)	31, 189, 708, 724, 997, 1200, 1514, 2049
BstMWI	GCNNNNNNNGC	3 cut(s)	64, 1082, 1913
BstNI	CCWGG	3 cut(s)	522, 656, 1296
BstSCI	CCNGG	3 cut(s)	520, 654, 1294
BstSFI	CTRYAG	1 cut(s)	1116
BstUI	CGCG	1 cut(s)	1907
BstV1I	GCAGC	2 cut(s)	611, 2037
BstV2I	GAAGAC	1 cut(s)	746
BstX2I	RGATCY	3 cut(s)	708, 724, 1514
BstYI	RGATCY	3 cut(s)	708, 724, 1514
Bsu36I	CCTNAGG	1 cut(s)	1791
BsuI	GTATCC	1 cut(s)	1257
BsuRI	GGCC	3 cut(s)	332, 617, 1110
BtgI	CCRYGG	1 cut(s)	569
BtrI	CACGTC	1 cut(s)	889
BtsCI	GGATG	9 cut(s)	418, 772, 784, 834, 901, 967, 1265, 1892, 1942
BtsI	GCAGTG	2 cut(s)	940, 1989
BtsIMutI	CAGTG	7 cut(s)	102, 232, 367, 636, 940, 1666, 1989
BveI	ACCTGC	1 cut(s)	49
Cac8I	GCNNGC	4 cut(s)	500, 853, 1317, 1840
CaiI	CAGNNNCTG	2 cut(s)	655, 1547
Cfr10I	RCCGGY	2 cut(s)	1867, 1912
Cfr13I	GGNCC	1 cut(s)	652
CseI	GACGC	3 cut(s)	1553, 1896, 1913
CsiI	ACCWGGT	2 cut(s)	654, 1294
Csp6I	GTAC	1 cut(s)	1277
CspAI	ACCGGT	1 cut(s)	1867
CviAII	CATG	4 cut(s)	570, 1001, 1097, 1801
CviQI	GTAC	1 cut(s)	1277
DdeI	CTNAG	8 cut(s)	180, 747, 807, 1005, 1511, 1658, 1791, 1996
DpnI	GATC	8 cut(s)	33, 191, 710, 726, 999, 1202, 1516, 2051
DpnII	GATC	8 cut(s)	31, 189, 708, 724, 997, 1200, 1514, 2049
DraI	TTTAAA	1 cut(s)	1557
Eam1104I	CTCTTC	3 cut(s)	319, 780, 1173
EarI	CTCTTC	3 cut(s)	319, 780, 1173
Eco130I	CCWWGG	4 cut(s)	569, 612, 1525, 1701
Eco47I	GGWCC	1 cut(s)	652
Eco57I	CTGAAG	1 cut(s)	208
Eco81I	CCTNAGG	1 cut(s)	1791
Eco88I	CYCGRG	2 cut(s)	1049, 1649
EcoNI	CCTNNNNNAGG	1 cut(s)	2040
EcoO109I	RGGNCCY	1 cut(s)	652
EcoRII	CCWGG	3 cut(s)	520, 654, 1294
EcoT14I	CCWWGG	4 cut(s)	569, 612, 1525, 1701
ErhI	CCWWGG	4 cut(s)	569, 612, 1525, 1701
FaeI	CATG	4 cut(s)	573, 1004, 1100, 1804
FalI	AAGNNNNNCTT	4 cut(s)	248, 280, 1933, 1965
FaqI	GGGAC	1 cut(s)	538
FatI	CATG	4 cut(s)	569, 1000, 1096, 1800
FauI	CCCGC	3 cut(s)	200, 958, 2039
Fnu4HI	GCNGC	2 cut(s)	625, 2026
FokI	GGATG	9 cut(s)	425, 779, 791, 841, 908, 974, 1272, 1879, 1949
Fsp4HI	GCNGC	2 cut(s)	625, 2026
FspBI	CTAG	3 cut(s)	77, 1211, 1674
GluI	GCNGC	2 cut(s)	625, 2026
GsuI	CTGGAG	3 cut(s)	147, 827, 918
HaeIII	GGCC	3 cut(s)	332, 617, 1110
HapII	CCGG	3 cut(s)	1192, 1868, 1913
HgaI	GACGC	3 cut(s)	1553, 1896, 1913
Hin1II	CATG	4 cut(s)	573, 1004, 1100, 1804
HincII	GTYRAC	3 cut(s)	637, 1306, 1903
HindII	GTYRAC	3 cut(s)	637, 1306, 1903
HindIII	AAGCTT	3 cut(s)	500, 903, 1763
HinfI	GANTC	9 cut(s)	80, 103, 215, 245, 365, 1033, 1690, 1810, 1855
HpaI	GTTAAC	2 cut(s)	637, 1306
HpaII	CCGG	3 cut(s)	1192, 1868, 1913
HphI	GGTGA	7 cut(s)	830, 1284, 1420, 1718, 1819, 1902, 1973
Hpy166II	GTNNAC	6 cut(s)	22, 54, 637, 800, 1306, 1903
Hpy188I	TCNGA	8 cut(s)	108, 181, 194, 220, 370, 886, 1580, 1999
Hpy188III	TCNNGA	7 cut(s)	77, 325, 844, 935, 995, 1049, 1173
Hpy8I	GTNNAC	6 cut(s)	22, 54, 637, 800, 1306, 1903
Hpy99I	CGWCG	1 cut(s)	1988
HpyAV	CCTTC	8 cut(s)	259, 322, 359, 656, 805, 886, 2054, 2060
HpyCH4III	ACNGT	3 cut(s)	631, 717, 1208
HpyCH4IV	ACGT	1 cut(s)	888
HpyF10VI	GCNNNNNNNGC	3 cut(s)	64, 1082, 1913
HpyF3I	CTNAG	8 cut(s)	180, 747, 807, 1005, 1511, 1658, 1791, 1996
HpySE526I	ACGT	1 cut(s)	888
Hsp92II	CATG	4 cut(s)	573, 1004, 1100, 1804
KspAI	GTTAAC	2 cut(s)	637, 1306
Kzo9I	GATC	8 cut(s)	31, 189, 708, 724, 997, 1200, 1514, 2049
LguI	GCTCTTC	1 cut(s)	780
LmnI	GCTCC	2 cut(s)	937, 1669
Lsp1109I	GCAGC	2 cut(s)	611, 2037
LweI	GCATC	5 cut(s)	386, 952, 1328, 1809, 1989
MabI	ACCWGGT	2 cut(s)	654, 1294
MaeI	CTAG	3 cut(s)	77, 1211, 1674
MaeII	ACGT	1 cut(s)	888
MaeIII	GTNAC	3 cut(s)	227, 1696, 1908
MalI	GATC	8 cut(s)	33, 191, 710, 726, 999, 1202, 1516, 2051
MboI	GATC	8 cut(s)	31, 189, 708, 724, 997, 1200, 1514, 2049
MfeI	CAATTG	1 cut(s)	1684
MflI	RGATCY	3 cut(s)	708, 724, 1514
MluI	ACGCGT	1 cut(s)	1905
MlyI	GAGTC	3 cut(s)	224, 1027, 1864
MmeI	TCCRAC	2 cut(s)	192, 736
MslI	CAYNNNNRTG	2 cut(s)	1465, 1805
MspA1I	CMGCKG	1 cut(s)	1929
MspCI	CTTAAG	3 cut(s)	202, 254, 734
MspI	CCGG	3 cut(s)	1192, 1868, 1913
MspR9I	CCNGG	3 cut(s)	522, 656, 1296
MunI	CAATTG	1 cut(s)	1684
Mva1269I	GAATGC	2 cut(s)	1460, 1738
MvaI	CCWGG	3 cut(s)	522, 656, 1296
MvnI	CGCG	1 cut(s)	1907
MwoI	GCNNNNNNNGC	3 cut(s)	64, 1082, 1913
NcoI	CCATGG	1 cut(s)	569
NdeII	GATC	8 cut(s)	31, 189, 708, 724, 997, 1200, 1514, 2049
NlaIII	CATG	4 cut(s)	573, 1004, 1100, 1804
NlaIV	GGNNCC	1 cut(s)	1671
NmuCI	GTSAC	2 cut(s)	227, 1908
PaeR7I	CTCGAG	2 cut(s)	1049, 1649
PciSI	GCTCTTC	1 cut(s)	780
PctI	GAATGC	2 cut(s)	1460, 1738
PfeI	GAWTC	6 cut(s)	80, 103, 245, 365, 1690, 1810
PflMI	CCANNNNNTGG	1 cut(s)	718
PinAI	ACCGGT	1 cut(s)	1867
PkrI	GCNGC	2 cut(s)	626, 2027
PleI	GAGTC	3 cut(s)	223, 1027, 1863
PpsI	GAGTC	3 cut(s)	223, 1027, 1863
PpuMI	RGGWCCY	1 cut(s)	652
PsiI	TTATAA	2 cut(s)	506, 704
Psp5II	RGGWCCY	1 cut(s)	652
Psp6I	CCWGG	3 cut(s)	520, 654, 1294
PspGI	CCWGG	3 cut(s)	520, 654, 1294
PspN4I	GGNNCC	1 cut(s)	1671
PspPI	GGNCC	1 cut(s)	652
PspPPI	RGGWCCY	1 cut(s)	652
PspXI	VCTCGAGB	1 cut(s)	1649
PstNI	CAGNNNCTG	2 cut(s)	655, 1547
PsuI	RGATCY	3 cut(s)	708, 724, 1514
RsaI	GTAC	1 cut(s)	1278
RsaNI	GTAC	1 cut(s)	1277
RseI	CAYNNNNRTG	2 cut(s)	1465, 1805
SapI	GCTCTTC	1 cut(s)	780
SatI	GCNGC	2 cut(s)	625, 2026
Sau3AI	GATC	8 cut(s)	31, 189, 708, 724, 997, 1200, 1514, 2049
Sau96I	GGNCC	1 cut(s)	652
ScaI	AGTACT	1 cut(s)	1278
SchI	GAGTC	3 cut(s)	224, 1027, 1864
ScrFI	CCNGG	3 cut(s)	522, 656, 1296
SexAI	ACCWGGT	2 cut(s)	654, 1294
SfaNI	GCATC	5 cut(s)	386, 952, 1328, 1809, 1989
SfcI	CTRYAG	1 cut(s)	1116
Sfr274I	CTCGAG	2 cut(s)	1049, 1649
SinI	GGWCC	1 cut(s)	652
SlaI	CTCGAG	2 cut(s)	1049, 1649
SmiMI	CAYNNNNRTG	2 cut(s)	1465, 1805
SmlI	CTYRAG	6 cut(s)	202, 254, 734, 1049, 1649, 1975
SmoI	CTYRAG	6 cut(s)	202, 254, 734, 1049, 1649, 1975
SsiI	CCGC	5 cut(s)	207, 951, 1929, 1959, 2046
SspI	AATATT	1 cut(s)	1092
SspMI	CTAG	3 cut(s)	77, 1211, 1674
StyD4I	CCNGG	3 cut(s)	520, 654, 1294
StyI	CCWWGG	4 cut(s)	569, 612, 1525, 1701
TaaI	ACNGT	3 cut(s)	631, 717, 1208
TaiI	ACGT	1 cut(s)	891
TaqI	TCGA	5 cut(s)	996, 1050, 1281, 1650, 1983
TatI	WGTACW	1 cut(s)	1276
TfiI	GAWTC	6 cut(s)	80, 103, 245, 365, 1690, 1810
TscAI	CASTG	7 cut(s)	102, 232, 367, 636, 947, 1666, 1996
TseFI	GTSAC	2 cut(s)	227, 1908
TseI	GCWGC	2 cut(s)	624, 2025
Tsp45I	GTSAC	2 cut(s)	227, 1908
TspGWI	ACGGA	1 cut(s)	1774
TspRI	CASTG	7 cut(s)	102, 232, 367, 636, 947, 1666, 1996
Van91I	CCANNNNNTGG	1 cut(s)	718
Vha464I	CTTAAG	3 cut(s)	202, 254, 734
VpaK11BI	GGWCC	1 cut(s)	652
XagI	CCTNNNNNAGG	1 cut(s)	2040
XapI	RAATTY	4 cut(s)	86, 832, 1397, 1965
XbaI	TCTAGA	1 cut(s)	76
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1103
XhoI	CTCGAG	2 cut(s)	1049, 1649
XspI	CTAG	3 cut(s)	77, 1211, 1674
ZrmI	AGTACT	1 cut(s)	1278

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.