Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004446

Physical Location & Seq

Genomic Coordinates Reverse (-)

30405357 .. 30411727

Gene Structure 6371 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1219400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1437 bp

ATGAACGAAAAGGCCAACGTCACCAAGGAGCTCAACGCCAAGCACAGAAAGATACTGGAAGGACTTCTTAAATTGCCAGAGAATAGAGAATGCGCTGACTGCAAAGCCAAGGGTCCAAGATGGGCTAGCGTGAATTTAGGTATTTTTGTATGCATGCAGTGTTCTGGGATCCACAGAAGTCTTGGGGTACACATATCCAAGGTGCGGTCTGCTACTTTGGACACATGGCTTCCAGAGCAGGTTGCTTTTATTCAATCAATGGGAAATGAGAAGTCAAATAGTTACTGGGAAGCAGAACTACCTCCAAACTATGACAGAGTTGGAATTGAAAATTTTATTCGTGCAAAGTATGAAGATAAGAGATGGATTCCTAGGGACGGACAAACAAAGCCACCTCCTAAAGTATGGGAAGAAAGGACTAACTTACATTGGCCTCAACCTGGAGAGAAAAGTGGGCATGGTTATGTTGGAAATTCTGAAAAGATGGTTGAGGAAAGGAAGAAAATTCAAACAACTCGTACAAAAGAAAGTGTTCCTGCTGCAAGAATTAGTCTTCCTGTTCCTCCGAAAGGTCCTGAACAGGTTGTACATGTCTCAAGACCACAAGATGTTCAGAAAATAGAACCAGCAGTTCCGCCAGCTGAAGCAACAAAACAAGCCATTGATACTGCTCCGCCTGCCCCTCCAAAAGTTGATTATGCTACTGACCTTTTCAACATGCTTTCAGTGGATGGTCCAAGTGAGAATGGGTCGGATGCAGCATCAGCTGAGGATAATGATTGGGCTGGTTTTCAGTCTGCTGAAAAGGCATCGACAGCTGAGAAAACTGGTCCATCAAAGGCAGCAGAGAACAATGTCCAATCTTCATCAGGAATTGAGGATCTATTTAAAGATGCACCTTCATTAACACCCGTTTCAGAGAAACCCCAGAAAGACTTAAAAAATGATATTATGAGTCTTTTTGAGAAGTCGAACATGGTGTCACCCTTCTCTATGCATCAGCAGCAACTAGCAATGCTGGCTCAGCAACAGTCTCTTCTCATGGCCGCGGCAGCTAAATCTGGCGCGGATCAAAAGTTTCCTGGTATTATTCAACAACCTGTGTCCAGTGGAAGCAATTTACCCTCCCAAAGTTGGCCAAACGTTGGCTACCAGTTTCCTGGATTGGTGATGCCTGTAGATGGGCAGGCTGCTCTACAGAATGTTTCACAGAGTCAGACAAAGACCATGGGACAGGCACATCCACTGGGGACCTCTGTTCCGTATTCAGCATCTAGCTTTTACAGTATGGCTCAAGTTACCCCAGTTAATGGCGTGACAAACGATGGGGCGAACAAGACTCAATCACCATCTCCCGTATTATCTTCAACTTCAGCACAGTCAGGAAAAGATTATGATTTCTCATCTTTAACACAATTGATGTTCGCAAAACCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001558 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0006810 GO:0007275 GO:0008150 GO:0009791 GO:0009838 GO:0009908 GO:0009987 GO:0010227 GO:0012505 GO:0016043 GO:0016192 GO:0022414 GO:0022607 GO:0030135 GO:0030136 GO:0030276 GO:0030308 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0034622 GO:0035459 GO:0035652 GO:0040008 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043547 GO:0043933 GO:0044085 GO:0044093 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045926 GO:0048367 GO:0048437 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051128 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0060858 GO:0061458 GO:0065003 GO:0065007 GO:0065009 GO:0071840 GO:0090567 GO:0090630 GO:0097708 GO:0098791 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

ArfGap

Protein Analysis

479

Amino Acids

52.0

Weight (kDa)

6.73

Isoelectric Point (pI)

55.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArfGap	PF01412	17 - 123	3e-41	Putative GTPase activating protein for Arf

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004446)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54310
fragaria_vesca	FvH4_3g20910 FvH4_3g20910 FvH4_3g20910 FvH4_3g20910 FvH4_3g20910 FvH4_3g20910
malus_domestica	MD03G1219400.v1.1 MD11G1237500.v1.1
prunus_persica	Prupe.4G189700_v2.0.a1 Prupe.4G189700_v2.0.a1 Prupe.4G189700_v2.0.a1
pyrus_communis	pycom03g16860 pycom11g20880
rosa_chinensis	RchiOBHm_Chr5g0035341
rosa_laevigata	RLG00000007187 RLG00000033618
rosa_multiflora	Rmu_sc0007948.1_g000014
rosa_roxburghii	Rroxscaffold_1G00045060 Rroxscaffold_5G00369750
rosa_rugosa	Rorug04G0221700 Rorug04G0221700 Rorug05G0151300 Rorug05G0151400 Rorug05G0151400
rosa_samantha	Rh4AG278900 Rh4BG284300 Rh5AG244400 Rh5BG243900 Rh5CG275700 Rh5DG252900
rosa_wichuraiana	Rw5G022320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	229
AccB7I	CCANNNNNTGG	2 cut(s)	1145, 1310
AccII	CGCG	2 cut(s)	1049, 1067
AciI	CCGC	6 cut(s)	205, 635, 674, 1047, 1049, 1067
AclI	AACGTT	1 cut(s)	1143
AclWI	GGATC	4 cut(s)	163, 176, 888, 1077
AcoI	YGGCCR	2 cut(s)	1044, 1136
AcsI	RAATTY	4 cut(s)	133, 331, 472, 504
AcuI	CTGAAG	2 cut(s)	663, 1356
AfaI	GTAC	3 cut(s)	189, 520, 588
AfiI	CCNNNNNNNGG	8 cut(s)	204, 377, 440, 569, 1134, 1145, 1181, 1310
AflIII	ACRYGT	1 cut(s)	589
AgsI	TTSAA	6 cut(s)	254, 329, 509, 715, 1094, 1368
AjnI	CCWGG	3 cut(s)	439, 1081, 1159
AluBI	AGCT	6 cut(s)	31, 641, 767, 818, 1055, 1278
AluI	AGCT	6 cut(s)	31, 641, 767, 818, 1055, 1278
Alw21I	GWGCWC	1 cut(s)	33
Alw26I	GTCTC	2 cut(s)	598, 1038
AlwI	GGATC	4 cut(s)	163, 176, 888, 1077
AoxI	GGCC	4 cut(s)	12, 431, 1044, 1136
ApeKI	GCWGC	6 cut(s)	539, 758, 842, 1003, 1052, 1190
ApoI	RAATTY	4 cut(s)	133, 331, 472, 504
ArsI	GACNNNNNNTTYG	2 cut(s)	965, 997
Asp700I	GAANNNNTTC	2 cut(s)	63, 531
AspA2I	CCTAGG	1 cut(s)	371
AspLEI	GCGC	2 cut(s)	95, 1067
AspS9I	GGNCC	5 cut(s)	113, 572, 734, 830, 1251
AsuHPI	GGTGA	4 cut(s)	13, 975, 1180, 1338
AsuNHI	GCTAGC	1 cut(s)	125
AvaII	GGWCC	5 cut(s)	113, 572, 734, 830, 1251
AvrII	CCTAGG	1 cut(s)	371
BalI	TGGCCA	1 cut(s)	1138
BamHI	GGATCC	1 cut(s)	168
BanII	GRGCYC	1 cut(s)	33
BarI	GAAGNNNNNNTAC	2 cut(s)	282, 314
BbsI	GAAGAC	1 cut(s)	545
Bbv12I	GWGCWC	1 cut(s)	33
BbvCI	CCTCAGC	1 cut(s)	768
BbvI	GCAGC	6 cut(s)	526, 770, 854, 1015, 1064, 1177
BccI	CCATC	8 cut(s)	114, 357, 478, 725, 841, 1175, 1319, 1357
BciT130I	CCWGG	3 cut(s)	441, 1083, 1161
BcoDI	GTCTC	2 cut(s)	598, 1038
BfaI	CTAG	4 cut(s)	126, 372, 1010, 1275
BfmI	CTRYAG	2 cut(s)	1176, 1196
BfuAI	ACCTGC	1 cut(s)	229
BisI	GCNGC	8 cut(s)	540, 759, 843, 1004, 1047, 1050, 1053, 1191
BlnI	CCTAGG	1 cut(s)	371
BlpI	GCTNAGC	1 cut(s)	1023
BlsI	GCNGC	8 cut(s)	541, 760, 844, 1005, 1048, 1051, 1054, 1192
Bme1390I	CCNGG	3 cut(s)	441, 1083, 1161
Bme18I	GGWCC	5 cut(s)	113, 572, 734, 830, 1251
BmgT120I	GGNCC	5 cut(s)	113, 572, 734, 830, 1251
BmiI	GGNNCC	3 cut(s)	114, 170, 1252
BmrFI	CCNGG	3 cut(s)	441, 1083, 1161
BmrI	ACTGGG	3 cut(s)	295, 1256, 1298
BmsI	GCATC	7 cut(s)	745, 770, 818, 883, 1006, 1161, 1280
BmtI	GCTAGC	1 cut(s)	129
BmuI	ACTGGG	3 cut(s)	295, 1256, 1298
BpiI	GAAGAC	1 cut(s)	545
BpmI	CTGGAG	1 cut(s)	462
Bpu10I	CCTNAGC	1 cut(s)	768
Bpu1102I	GCTNAGC	1 cut(s)	1023
BpuEI	CTTGAG	2 cut(s)	580, 1278
BsaJI	CCNNGG	6 cut(s)	24, 108, 198, 371, 1047, 1227
Bsc4I	CCNNNNNNNGG	8 cut(s)	204, 377, 440, 569, 1134, 1145, 1181, 1310
Bse1I	ACTGG	7 cut(s)	60, 290, 832, 1107, 1153, 1251, 1304
Bse3DI	GCAATG	1 cut(s)	1020
BseBI	CCWGG	3 cut(s)	441, 1083, 1161
BseDI	CCNNGG	6 cut(s)	24, 108, 198, 371, 1047, 1227
BseGI	GGATG	3 cut(s)	736, 760, 1240
BseLI	CCNNNNNNNGG	8 cut(s)	204, 377, 440, 569, 1134, 1145, 1181, 1310
BseMI	GCAATG	1 cut(s)	1020
BseMII	CTCAG	3 cut(s)	759, 810, 1037
BseNI	ACTGG	7 cut(s)	60, 290, 832, 1107, 1153, 1251, 1304
BseXI	GCAGC	6 cut(s)	526, 770, 854, 1015, 1064, 1177
Bsh1236I	CGCG	2 cut(s)	1049, 1067
BshFI	GGCC	4 cut(s)	14, 433, 1046, 1138
BsiHKAI	GWGCWC	1 cut(s)	33
BslFI	GGGAC	3 cut(s)	389, 1245, 1264
BslI	CCNNNNNNNGG	8 cut(s)	204, 377, 440, 569, 1134, 1145, 1181, 1310
BsmAI	GTCTC	2 cut(s)	598, 1038
BsmFI	GGGAC	3 cut(s)	389, 1245, 1264
BsmI	GAATGC	1 cut(s)	95
BsnI	GGCC	4 cut(s)	14, 433, 1046, 1138
Bsp1286I	GDGCHC	1 cut(s)	33
Bsp1407I	TGTACA	1 cut(s)	586
Bsp143I	GATC	3 cut(s)	168, 880, 1069
Bsp1720I	GCTNAGC	1 cut(s)	1023
Bsp19I	CCATGG	1 cut(s)	1227
BspACI	CCGC	6 cut(s)	205, 635, 674, 1047, 1049, 1067
BspANI	GGCC	4 cut(s)	14, 433, 1046, 1138
BspCNI	CTCAG	3 cut(s)	760, 811, 1036
BspFNI	CGCG	2 cut(s)	1049, 1067
BspLI	GGNNCC	3 cut(s)	114, 170, 1252
BspMI	ACCTGC	1 cut(s)	229
BspOI	GCTAGC	1 cut(s)	129
BspPI	GGATC	4 cut(s)	163, 176, 888, 1077
BsrDI	GCAATG	1 cut(s)	1020
BsrGI	TGTACA	1 cut(s)	586
BsrI	ACTGG	7 cut(s)	60, 290, 832, 1107, 1153, 1251, 1304
BssECI	CCNNGG	6 cut(s)	24, 108, 198, 371, 1047, 1227
BssMI	GATC	3 cut(s)	168, 880, 1069
BssT1I	CCWWGG	5 cut(s)	24, 108, 198, 371, 1227
Bst2UI	CCWGG	3 cut(s)	441, 1083, 1161
Bst4CI	ACNGT	4 cut(s)	1032, 1286, 1380, 1434
Bst6I	CTCTTC	1 cut(s)	1041
BstAUI	TGTACA	1 cut(s)	586
BstC8I	GCNNGC	6 cut(s)	127, 155, 639, 678, 1020, 1188
BstDEI	CTNAG	3 cut(s)	768, 819, 1023
BstDSI	CCRYGG	2 cut(s)	1047, 1227
BstENI	CCTNNNNNAGG	1 cut(s)	567
BstF5I	GGATG	3 cut(s)	736, 760, 1240
BstFNI	CGCG	2 cut(s)	1049, 1067
BstHHI	GCGC	2 cut(s)	95, 1067
BstKTI	GATC	3 cut(s)	171, 883, 1072
BstMAI	GTCTC	2 cut(s)	598, 1038
BstMBI	GATC	3 cut(s)	168, 880, 1069
BstNI	CCWGG	3 cut(s)	441, 1083, 1161
BstNSI	RCATGY	3 cut(s)	157, 593, 721
BstSCI	CCNGG	3 cut(s)	439, 1081, 1159
BstSFI	CTRYAG	2 cut(s)	1176, 1196
BstUI	CGCG	2 cut(s)	1049, 1067
BstV1I	GCAGC	6 cut(s)	526, 770, 854, 1015, 1064, 1177
BstV2I	GAAGAC	1 cut(s)	545
BstX2I	RGATCY	2 cut(s)	168, 880
BstXI	CCANNNNNNTGG	1 cut(s)	1160
BstYI	RGATCY	2 cut(s)	168, 880
BsuRI	GGCC	4 cut(s)	14, 433, 1046, 1138
BtgI	CCRYGG	2 cut(s)	1047, 1227
BtsCI	GGATG	3 cut(s)	736, 760, 1240
BtsI	GCAGTG	1 cut(s)	164
BtsIMutI	CAGTG	4 cut(s)	164, 732, 1114, 1244
BveI	ACCTGC	1 cut(s)	229
Cac8I	GCNNGC	6 cut(s)	127, 155, 639, 678, 1020, 1188
CfoI	GCGC	2 cut(s)	95, 1067
Cfr13I	GGNCC	5 cut(s)	113, 572, 734, 830, 1251
Cfr42I	CCGCGG	1 cut(s)	1050
Csp6I	GTAC	3 cut(s)	188, 519, 587
CviAII	CATG	8 cut(s)	154, 225, 458, 590, 718, 976, 1042, 1228
CviQI	GTAC	3 cut(s)	188, 519, 587
DdeI	CTNAG	3 cut(s)	768, 819, 1023
DpnI	GATC	3 cut(s)	170, 882, 1071
DpnII	GATC	3 cut(s)	168, 880, 1069
DraI	TTTAAA	1 cut(s)	889
EaeI	YGGCCR	2 cut(s)	1044, 1136
Eam1104I	CTCTTC	1 cut(s)	1041
EarI	CTCTTC	1 cut(s)	1041
EciI	GGCGGA	2 cut(s)	624, 663
Ecl136II	GAGCTC	1 cut(s)	31
Eco130I	CCWWGG	5 cut(s)	24, 108, 198, 371, 1227
Eco24I	GRGCYC	1 cut(s)	33
Eco47I	GGWCC	5 cut(s)	113, 572, 734, 830, 1251
Eco53kI	GAGCTC	1 cut(s)	31
Eco57I	CTGAAG	2 cut(s)	663, 1356
EcoICRI	GAGCTC	1 cut(s)	31
EcoNI	CCTNNNNNAGG	1 cut(s)	567
EcoO109I	RGGNCCY	2 cut(s)	572, 1251
EcoRII	CCWGG	3 cut(s)	439, 1081, 1159
EcoT14I	CCWWGG	5 cut(s)	24, 108, 198, 371, 1227
EcoT22I	ATGCAT	2 cut(s)	155, 999
EcoT38I	GRGCYC	1 cut(s)	33
ErhI	CCWWGG	5 cut(s)	24, 108, 198, 371, 1227
FaeI	CATG	8 cut(s)	157, 228, 461, 593, 721, 979, 1045, 1231
FalI	AAGNNNNNCTT	2 cut(s)	51, 83
FaqI	GGGAC	3 cut(s)	389, 1245, 1264
FatI	CATG	8 cut(s)	153, 224, 457, 589, 717, 975, 1041, 1227
Fnu4HI	GCNGC	8 cut(s)	540, 759, 843, 1004, 1047, 1050, 1053, 1191
FokI	GGATG	3 cut(s)	743, 767, 1227
FriOI	GRGCYC	1 cut(s)	33
Fsp4HI	GCNGC	8 cut(s)	540, 759, 843, 1004, 1047, 1050, 1053, 1191
FspBI	CTAG	4 cut(s)	126, 372, 1010, 1275
GlaI	GCGC	2 cut(s)	94, 1066
GluI	GCNGC	8 cut(s)	540, 759, 843, 1004, 1047, 1050, 1053, 1191
GsuI	CTGGAG	1 cut(s)	462
HaeIII	GGCC	4 cut(s)	14, 433, 1046, 1138
HhaI	GCGC	2 cut(s)	95, 1067
Hin1II	CATG	8 cut(s)	157, 228, 461, 593, 721, 979, 1045, 1231
Hin6I	GCGC	2 cut(s)	93, 1065
HinP1I	GCGC	2 cut(s)	93, 1065
HinfI	GANTC	4 cut(s)	367, 955, 1213, 1339
HphI	GGTGA	4 cut(s)	13, 975, 1180, 1338
Hpy166II	GTNNAC	1 cut(s)	190
Hpy188I	TCNGA	6 cut(s)	478, 567, 615, 754, 919, 1218
Hpy188III	TCNNGA	5 cut(s)	233, 575, 597, 870, 1383
Hpy8I	GTNNAC	1 cut(s)	190
HpyAV	CCTTC	3 cut(s)	53, 909, 997
HpyCH4III	ACNGT	4 cut(s)	1032, 1286, 1380, 1434
HpyCH4IV	ACGT	2 cut(s)	18, 1143
HpyCH4V	TGCA	8 cut(s)	102, 153, 157, 344, 542, 758, 896, 997
HpyF3I	CTNAG	3 cut(s)	768, 819, 1023
HpySE526I	ACGT	2 cut(s)	18, 1143
Hsp92II	CATG	8 cut(s)	157, 228, 461, 593, 721, 979, 1045, 1231
HspAI	GCGC	2 cut(s)	93, 1065
KspI	CCGCGG	1 cut(s)	1050
Kzo9I	GATC	3 cut(s)	168, 880, 1069
LmnI	GCTCC	2 cut(s)	28, 676
Lsp1109I	GCAGC	6 cut(s)	526, 770, 854, 1015, 1064, 1177
LweI	GCATC	7 cut(s)	745, 770, 818, 883, 1006, 1161, 1280
MaeI	CTAG	4 cut(s)	126, 372, 1010, 1275
MaeII	ACGT	2 cut(s)	18, 1143
MaeIII	GTNAC	5 cut(s)	19, 281, 981, 1297, 1315
MalI	GATC	3 cut(s)	170, 882, 1071
MboI	GATC	3 cut(s)	168, 880, 1069
MboII	GAAGA	7 cut(s)	365, 422, 511, 545, 855, 1028, 1356
MfeI	CAATTG	1 cut(s)	1415
MflI	RGATCY	2 cut(s)	168, 880
MhlI	GDGCHC	1 cut(s)	33
MlsI	TGGCCA	1 cut(s)	1138
MluNI	TGGCCA	1 cut(s)	1138
MlyI	GAGTC	3 cut(s)	964, 1222, 1333
MmeI	TCCRAC	3 cut(s)	301, 448, 732
Mox20I	TGGCCA	1 cut(s)	1138
Mph1103I	ATGCAT	2 cut(s)	155, 999
MroXI	GAANNNNTTC	2 cut(s)	63, 531
MscI	TGGCCA	1 cut(s)	1138
MseI	TTAA	6 cut(s)	69, 888, 905, 938, 1308, 1409
MslI	CAYNNNNRTG	1 cut(s)	462
Msp20I	TGGCCA	1 cut(s)	1138
MspA1I	CMGCKG	4 cut(s)	641, 767, 818, 1049
MspR9I	CCNGG	3 cut(s)	441, 1083, 1161
MunI	CAATTG	1 cut(s)	1415
Mva1269I	GAATGC	1 cut(s)	95
MvaI	CCWGG	3 cut(s)	441, 1083, 1161
MvnI	CGCG	2 cut(s)	1049, 1067
NcoI	CCATGG	1 cut(s)	1227
NdeII	GATC	3 cut(s)	168, 880, 1069
NheI	GCTAGC	1 cut(s)	125
NlaIII	CATG	8 cut(s)	157, 228, 461, 593, 721, 979, 1045, 1231
NlaIV	GGNNCC	3 cut(s)	114, 170, 1252
NmuCI	GTSAC	3 cut(s)	19, 981, 1315
NsiI	ATGCAT	2 cut(s)	155, 999
NspI	RCATGY	3 cut(s)	157, 593, 721
PaeI	GCATGC	1 cut(s)	157
PciI	ACATGT	1 cut(s)	589
PctI	GAATGC	1 cut(s)	95
PdmI	GAANNNNTTC	2 cut(s)	63, 531
PfeI	GAWTC	1 cut(s)	367
PflMI	CCANNNNNTGG	2 cut(s)	1145, 1310
PfoI	TCCNGGA	1 cut(s)	1159
PkrI	GCNGC	8 cut(s)	541, 760, 844, 1005, 1048, 1051, 1054, 1192
PleI	GAGTC	3 cut(s)	963, 1221, 1333
PpsI	GAGTC	3 cut(s)	963, 1221, 1333
PpuMI	RGGWCCY	2 cut(s)	572, 1251
PscI	ACATGT	1 cut(s)	589
Psp124BI	GAGCTC	1 cut(s)	33
Psp1406I	AACGTT	1 cut(s)	1143
Psp5II	RGGWCCY	2 cut(s)	572, 1251
Psp6I	CCWGG	3 cut(s)	439, 1081, 1159
PspGI	CCWGG	3 cut(s)	439, 1081, 1159
PspN4I	GGNNCC	3 cut(s)	114, 170, 1252
PspPI	GGNCC	5 cut(s)	113, 572, 734, 830, 1251
PspPPI	RGGWCCY	2 cut(s)	572, 1251
PsrI	GAACNNNNNNTAC	2 cut(s)	570, 602
PsuI	RGATCY	2 cut(s)	168, 880
PvuII	CAGCTG	3 cut(s)	641, 767, 818
RsaI	GTAC	3 cut(s)	189, 520, 588
RsaNI	GTAC	3 cut(s)	188, 519, 587
RseI	CAYNNNNRTG	1 cut(s)	462
SacI	GAGCTC	1 cut(s)	33
SacII	CCGCGG	1 cut(s)	1050
SaqAI	TTAA	6 cut(s)	69, 888, 905, 938, 1308, 1409
SatI	GCNGC	8 cut(s)	540, 759, 843, 1004, 1047, 1050, 1053, 1191
Sau3AI	GATC	3 cut(s)	168, 880, 1069
Sau96I	GGNCC	5 cut(s)	113, 572, 734, 830, 1251
SchI	GAGTC	3 cut(s)	964, 1222, 1333
ScrFI	CCNGG	3 cut(s)	441, 1083, 1161
SduI	GDGCHC	1 cut(s)	33
SfaNI	GCATC	7 cut(s)	745, 770, 818, 883, 1006, 1161, 1280
SfcI	CTRYAG	2 cut(s)	1176, 1196
Sfr303I	CCGCGG	1 cut(s)	1050
SgrBI	CCGCGG	1 cut(s)	1050
SinI	GGWCC	5 cut(s)	113, 572, 734, 830, 1251
SmiMI	CAYNNNNRTG	1 cut(s)	462
SmlI	CTYRAG	2 cut(s)	595, 1293
SmoI	CTYRAG	2 cut(s)	595, 1293
SphI	GCATGC	1 cut(s)	157
SsiI	CCGC	6 cut(s)	205, 635, 674, 1047, 1049, 1067
SspMI	CTAG	4 cut(s)	126, 372, 1010, 1275
SstI	GAGCTC	1 cut(s)	33
StyD4I	CCNGG	3 cut(s)	439, 1081, 1159
StyI	CCWWGG	5 cut(s)	24, 108, 198, 371, 1227
TaaI	ACNGT	4 cut(s)	1032, 1286, 1380, 1434
TaiI	ACGT	2 cut(s)	21, 1146
TaqI	TCGA	2 cut(s)	812, 971
TatI	WGTACW	1 cut(s)	586
TauI	GCSGC	2 cut(s)	1049, 1052
TfiI	GAWTC	1 cut(s)	367
Tru1I	TTAA	6 cut(s)	69, 888, 905, 938, 1308, 1409
Tru9I	TTAA	6 cut(s)	69, 888, 905, 938, 1308, 1409
TscAI	CASTG	4 cut(s)	164, 732, 1114, 1251
TseFI	GTSAC	3 cut(s)	19, 981, 1315
TseI	GCWGC	6 cut(s)	539, 758, 842, 1003, 1052, 1190
Tsp45I	GTSAC	3 cut(s)	19, 981, 1315
TspDTI	ATGAA	4 cut(s)	17, 366, 855, 891
TspGWI	ACGGA	2 cut(s)	393, 1251
TspRI	CASTG	4 cut(s)	164, 732, 1114, 1251
Van91I	CCANNNNNTGG	2 cut(s)	1145, 1310
VpaK11BI	GGWCC	5 cut(s)	113, 572, 734, 830, 1251
XagI	CCTNNNNNAGG	1 cut(s)	567
XapI	RAATTY	4 cut(s)	133, 331, 472, 504
XceI	RCATGY	3 cut(s)	157, 593, 721
XcmI	CCANNNNNNNNNTGG	1 cut(s)	179
XmaJI	CCTAGG	1 cut(s)	371
XmnI	GAANNNNTTC	2 cut(s)	63, 531
XspI	CTAG	4 cut(s)	126, 372, 1010, 1275
Zsp2I	ATGCAT	2 cut(s)	155, 999

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.