Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004410

Physical Location & Seq

Genomic Coordinates Reverse (-)

30498591 .. 30501639

Gene Structure 3049 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1221300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2787 bp

ATGGTTACAACCGCACTAAAAAGGCTGAAGCTTGATGGGTTTTCATGGTGGGATTCACGTAGTCTCTGTGTCAACTGGGTTTTCTCTTGGCGCTGGCCCAATTCACTCTGCTGCGGATTTTTCCCATTTTTCACTTCCATTGAGGTAAAAGCTCAAATCTGTGTGTATTTTTGTGTTGGAGAAGTTGGTGGTGCAAGTGGTACTTTCCTCTGCATACGAGCAATGTTTATTTGGATGGATCTGGGATCTGGTTTTTCAGACACGGTGAAGATGAAACTTGTAACTTGGGTAGCTTTGTTTCTTGCTGTTGTTGTGTTCATGAGCTATAGATCATCCGCTTCATTCACTCCTGTCGATAACTACTTAATTGCCTGCGGTTCTCCGAAAAATGTCACATTCCAAGGTCGAACCTTTGTTCCCGATACACAACAATCTTCCCTTGTACTAAAAAGTGCAAATTCTTTAGTTGCCAGCCCCAATGCTAATGCCCCTTCTCCAATTTACCAATCTGCTCGAGTTTTCCAGGCAACAGCTTCTTACAAATTCAAAGTCCAGCAAGAAGGCCGGCATTGGGTCCGCCTTTATTTTTTCCCTCTTACAAGCCCTGGCCAAAAATTGGATTCTGCTCAAATGACTGTAGTTACTGAAAATTTTGTACTCTTGAACAACTTCACTTTCAAGAACTTTAACGGTTCTTATCTGTTCAAGGAGTATGCAATTAATGTAACTTCGGATACTTTGACTCTTAATTTCATTCCTTCAAACAATTCAGTTGCTTTTGTTAATGCAATTGAAGTTGTTTCTATCCCGGATGCGCTGCTCCCTGACCAGGCATATGCTGTGAATCCATCTGCTCCTTTTAGCGGACTTTCTGAGCTTGCCCTTCAAACAGTTTACCGGTTAAACATGGGGGGCCCTTTGCTCACATCTCAAAATGATACCCTTGGAAGAACTTGGGAGAATGACGTGAAGTACCTCCATGTGGACAGTTCTGCAGTGAATGTATCGGTGAACCTTGCAAGCATAAAATATCCGCCGACTGTCACACCTGAAATTGCCCCAAATTGGGTCTATGCCACAGCTGAAGCCATGGGAAATGCAAACGTGCCCAATGTGAACTTCAACATAACTTGGGTCTTTACAGTAGATCCAAATTTTATGTATCTTGTTCGGGTACACTTTTGTGATATTGTCAGCAAGGCTCTGAATAATCTCGTTTTCAATGTTTTCATAAATTCTGACAATGTGCTTGGGAGTCTTGATCTCTCTTCGATGACTGGTGACTTGGGCGTGCCATATTACAAAGACTTTGTTTCCAACTCCACAGAAGATGCAGGTACTTTGACTATCAGTGTTGGTCCAGATTCATCAGCTGATATTACCAATGCAATTCTGAATGGGTTGGAGATTATGAAGATCAGCAATGAGTTGGGGAGCTTGGACGGGTCTTTATCCGTGGGGAATCTTCTTCCTAGTCCATCCTCAAAGAAGAATAATATAGGAATCATAGTTGGCTCTGTTGTGGGATCTGTTGCTGTTGTGGCAATTATTGGTTTCCTTTATTGCTGCTTGGCATCCCGCAAGCCGAAGACAACTAGCCAAGCAACATGGCTGCCCTTGCCCTTGTACGGAAATTCTCAGACCATGACAAAAATGTCTACAACTTCACAAAAGAGTGGAACAGCTAGCTGCATTTCATTAGCTTCCTCCAATCTTGGCAAAATCTTCATGTTCCAGGAGATCCTGGATGCAACCAATAAGTTCGATGAGAAGGCACTTCTTGGGGTTGGTGGTTTTGGCAGGGTTTACAAGGGAACACTTGAAGATGGGACTAAAGTAGCTGTCAAAAGGGGAAACCCCAGATCTGAACAAGGTCTTGCTGAATTCCGAACTGAGATTGAAATGTTATCCAAGCTCCGCCACCGCCACCTTGTGTCTCTTATTGGCTACTGTGATGAAAGGTCAGAAATGATTCTTGTCTATGAATACATGGCCAATGGACCCCTCAGGAGCCATTTGTATGGAACAGACCTGCCAACACTCTCATGGAAGCTACGCCTTGAAATTTGCATTGGGGCTGCAAGAGGGCTCCATTATCTCCACACTGGTGCAGCTCAAAGCATAATTCACCGAGATGTGAAGACAACCAACATTCTCTTGGATGAGAACTTTGTAGCCAAAGTTGCTGATTTTGGCCTCTCAAAAGCAGGTCCAGCTCTAGATCAGACCCATGTCAGTACAGCTGTTAAGGGTAGTTTTGGTTACCTTGATCCTGAATACTTCCGAAGGCAACAGCTCACGGAGAAATCTGATGTGTATTCGTTCGGGGTAGTTCTAATGGAAGTTCTGTGCACAAGACCAGCTTTAAATCCTGTTCTTCCAAGGGAGCAGGTCAACATAGCAGAGTGGGCGATGACATGGCAGAAGAAGGGCATGCTAGACCAAATCATGGACCCTAATCTGGCGGGGAAGGTAAATCCAGCTTCTCTTAAGAAGTTCGGGGAGACAGCTGAGAAGTGCCTTGCTGAGTATGGTGTTGACAGGCCATCAATGGGAGATGTCTTGTGGAATCTTGAATATGCTCTTCAGCTCGAGGAGACATCATCTGCACTCATGGAACCTGACGATAACAGCACAAACCACATACCTGGTATCCAATTAACCCAAGGAGAGCCATTTGATAACAGTGTGAGCATGGTTGACGGGGGACACTCCGGCACAGATGACACAGAAAATGCTGCCACAAGTGCTGTGTTTTCTCAGCTTGTTAATCCTCGCGGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

929

Amino Acids

101.72

Weight (kDa)

5.77

Isoelectric Point (pI)

34.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	123 - 473	2.7e-31	Malectin-like domain
Pkinase	PF00069	589 - 851	2.1e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	592 - 785	2.6e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54380
fragaria_vesca	FvH4_2g16820 FvH4_2g16820 FvH4_3g20780 FvH4_3g20780 FvH4_3g20780
malus_domestica	MD03G1221300.v1.1 MD11G1238700.v1.1
prunus_persica	Prupe.4G188000_v2.0.a1
pyrus_communis	pycom03g17020 pycom11g21030
rosa_chinensis	RchiOBHm_Chr5g0035071 RchiOBHm_Chr6g0280941
rosa_laevigata	RLG00000013014 RLG00000033592
rosa_multiflora	Rmu_sc0005362.1_g000028 Rmu_sc0023551.1_g000001 Rmu_sc0023552.1_g000001
rosa_roxburghii	Rroxscaffold_1G00045340 Rroxscaffold_7G00187470
rosa_rugosa	Rorug05G0149600 Rorug06G0137100
rosa_samantha	Rh5AG242600 Rh5BG242200 Rh5CG273700 Rh5DG250800 Rh6AG249400 Rh6BG251500 Rh6CG252100 Rh6DG243900
rosa_wichuraiana	Rw5G022160 Rw6G021580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1654
Acc36I	ACCTGC	4 cut(s)	1325, 2040, 2198, 2380
AccB7I	CCANNNNNTGG	2 cut(s)	616, 2449
AccI	GTMKAC	1 cut(s)	1658
AccII	CGCG	1 cut(s)	2778
AclWI	GGATC	6 cut(s)	246, 253, 1142, 1534, 1735, 2264
AcoI	YGGCCR	2 cut(s)	607, 1992
AcsI	RAATTY	8 cut(s)	457, 542, 649, 1153, 1234, 1633, 1883, 2064
AcuI	CTGAAG	3 cut(s)	47, 1104, 2570
AdeI	CACNNNGTG	1 cut(s)	1933
AfaI	GTAC	8 cut(s)	202, 444, 657, 974, 1176, 1339, 1628, 2239
AflII	CTTAAG	1 cut(s)	2489
AgeI	ACCGGT	1 cut(s)	897
AjiI	CACGTC	1 cut(s)	967
AjnI	CCWGG	6 cut(s)	522, 604, 828, 1734, 1743, 2647
AjuI	GAANNNNNNNTTGG	4 cut(s)	1103, 1135, 2055, 2087
AleI	CACNNNNGTG	2 cut(s)	1182, 2106
AloI	GAACNNNNNNTCC	2 cut(s)	400, 432
Alw21I	GWGCWC	1 cut(s)	2354
Alw26I	GTCTC	4 cut(s)	68, 1941, 2498, 2591
Alw44I	GTGCAC	1 cut(s)	2350
AlwI	GGATC	6 cut(s)	246, 253, 1142, 1534, 1735, 2264
Ama87I	CYCGRG	2 cut(s)	513, 2591
AoxI	GGCC	7 cut(s)	95, 562, 607, 913, 1992, 2194, 2543
ApaI	GGGCCC	1 cut(s)	917
ApaLI	GTGCAC	1 cut(s)	2350
ApeKI	GCWGC	8 cut(s)	111, 817, 1566, 1612, 1689, 2078, 2111, 2738
ApoI	RAATTY	8 cut(s)	457, 542, 649, 1153, 1234, 1633, 1883, 2064
ArsI	GACNNNNNNTTYG	2 cut(s)	534, 566
AseI	ATTAAT	1 cut(s)	720
AsiGI	ACCGGT	1 cut(s)	897
Asp700I	GAANNNNTTC	2 cut(s)	668, 1971
AspLEI	GCGC	2 cut(s)	93, 817
AspS9I	GGNCC	8 cut(s)	96, 574, 913, 914, 1358, 2000, 2210, 2452
AsuC2I	CCSGG	1 cut(s)	809
AsuHPI	GGTGA	4 cut(s)	277, 1021, 1292, 2120
AsuNHI	GCTAGC	1 cut(s)	1685
AvaI	CYCGRG	2 cut(s)	513, 2591
AvaII	GGWCC	5 cut(s)	574, 1358, 2000, 2210, 2452
AxyI	CCTNAGG	1 cut(s)	2006
BaeGI	GKGCMC	3 cut(s)	917, 1110, 2354
BalI	TGGCCA	2 cut(s)	609, 1994
BanII	GRGCYC	2 cut(s)	917, 2091
BbsI	GAAGAC	2 cut(s)	1595, 2147
Bbv12I	GWGCWC	1 cut(s)	2354
BbvI	GCAGC	8 cut(s)	98, 804, 1553, 1599, 1676, 2065, 2123, 2725
BccI	CCATC	6 cut(s)	29, 229, 856, 1486, 1820, 2554
BciT130I	CCWGG	6 cut(s)	524, 606, 830, 1736, 1745, 2649
BciVI	GTATCC	2 cut(s)	727, 2663
BcnI	CCSGG	1 cut(s)	809
BcoDI	GTCTC	4 cut(s)	68, 1941, 2498, 2591
BfaI	CTAG	5 cut(s)	1473, 1596, 1686, 2219, 2438
BfmI	CTRYAG	3 cut(s)	325, 636, 993
BfoI	RGCGCY	1 cut(s)	94
BfrI	CTTAAG	1 cut(s)	2489
BfuAI	ACCTGC	4 cut(s)	1325, 2040, 2198, 2380
BfuI	GTATCC	2 cut(s)	727, 2663
BglII	AGATCT	1 cut(s)	1862
BisI	GCNGC	8 cut(s)	112, 818, 1567, 1613, 1690, 2079, 2112, 2739
BlsI	GCNGC	8 cut(s)	113, 819, 1568, 1614, 1691, 2080, 2113, 2740
Bme1390I	CCNGG	7 cut(s)	524, 606, 809, 830, 1736, 1745, 2649
Bme18I	GGWCC	5 cut(s)	574, 1358, 2000, 2210, 2452
BmeT110I	CYCGRG	2 cut(s)	513, 2591
BmgBI	CACGTC	1 cut(s)	967
BmgT120I	GGNCC	8 cut(s)	96, 574, 913, 914, 1358, 2000, 2210, 2452
BmiI	GGNNCC	8 cut(s)	575, 914, 915, 2002, 2012, 2090, 2454, 2619
BmrFI	CCNGG	7 cut(s)	524, 606, 809, 830, 1736, 1745, 2649
BmrI	ACTGGG	1 cut(s)	85
BmsI	GCATC	4 cut(s)	802, 1321, 1583, 1738
BmtI	GCTAGC	1 cut(s)	1689
BmuI	ACTGGG	1 cut(s)	85
BoxI	GACNNNNGTC	1 cut(s)	2231
BpiI	GAAGAC	2 cut(s)	1595, 2147
BpuMI	CCSGG	1 cut(s)	809
BsaAI	YACGTR	1 cut(s)	59
BsaJI	CCNNGG	7 cut(s)	400, 604, 943, 1089, 1455, 2381, 2665
BsaWI	WCCGGW	1 cut(s)	897
Bse118I	RCCGGY	2 cut(s)	564, 897
Bse1I	ACTGG	3 cut(s)	80, 1282, 2110
Bse21I	CCTNAGG	1 cut(s)	2006
Bse3DI	GCAATG	2 cut(s)	228, 1429
BseBI	CCWGG	6 cut(s)	524, 606, 830, 1736, 1745, 2649
BseDI	CCNNGG	7 cut(s)	400, 604, 943, 1089, 1455, 2381, 2665
BseGI	GGATG	7 cut(s)	240, 332, 817, 1478, 1574, 1753, 2167
BseMI	GCAATG	2 cut(s)	228, 1429
BseMII	CTCAG	7 cut(s)	864, 1652, 1884, 2020, 2502, 2517, 2774
BseNI	ACTGG	3 cut(s)	80, 1282, 2110
BseRI	GAGGAG	1 cut(s)	2609
BseSI	GKGCMC	3 cut(s)	917, 1110, 2354
BseXI	GCAGC	8 cut(s)	98, 804, 1553, 1599, 1676, 2065, 2123, 2725
BsgI	GTGCAG	2 cut(s)	2130, 2592
Bsh1236I	CGCG	1 cut(s)	2778
BshFI	GGCC	7 cut(s)	97, 564, 609, 915, 1994, 2196, 2545
BshTI	ACCGGT	1 cut(s)	897
BsiHKAI	GWGCWC	1 cut(s)	2354
BsiHKCI	CYCGRG	2 cut(s)	513, 2591
BsiSI	CCGG	4 cut(s)	565, 809, 898, 2715
BslFI	GGGAC	2 cut(s)	1843, 2721
BsmAI	GTCTC	4 cut(s)	68, 1941, 2498, 2591
BsmFI	GGGAC	2 cut(s)	1843, 2721
BsnI	GGCC	7 cut(s)	97, 564, 609, 915, 1994, 2196, 2545
BsoBI	CYCGRG	2 cut(s)	513, 2591
Bsp120I	GGGCCC	1 cut(s)	913
Bsp1286I	GDGCHC	4 cut(s)	917, 1110, 2091, 2354
Bsp19I	CCATGG	1 cut(s)	1089
BspANI	GGCC	7 cut(s)	97, 564, 609, 915, 1994, 2196, 2545
BspCNI	CTCAG	7 cut(s)	865, 1651, 1885, 2019, 2503, 2518, 2773
BspFNI	CGCG	1 cut(s)	2778
BspHI	TCATGA	1 cut(s)	318
BspLI	GGNNCC	8 cut(s)	575, 914, 915, 2002, 2012, 2090, 2454, 2619
BspMAI	CTGCAG	1 cut(s)	997
BspMI	ACCTGC	4 cut(s)	1325, 2040, 2198, 2380
BspOI	GCTAGC	1 cut(s)	1689
BspPI	GGATC	6 cut(s)	246, 253, 1142, 1534, 1735, 2264
BspQI	GCTCTTC	1 cut(s)	2589
BspTI	CTTAAG	1 cut(s)	2489
BsrDI	GCAATG	2 cut(s)	228, 1429
BsrFI	RCCGGY	2 cut(s)	564, 897
BsrI	ACTGG	3 cut(s)	80, 1282, 2110
BssAI	RCCGGY	2 cut(s)	564, 897
BssECI	CCNNGG	7 cut(s)	400, 604, 943, 1089, 1455, 2381, 2665
BssT1I	CCWWGG	5 cut(s)	400, 943, 1089, 2381, 2665
Bst2UI	CCWGG	6 cut(s)	524, 606, 830, 1736, 1745, 2649
Bst4CI	ACNGT	9 cut(s)	265, 637, 692, 892, 989, 1042, 1144, 1952, 2687
Bst6I	CTCTTC	2 cut(s)	1273, 2589
BstAFI	CTTAAG	1 cut(s)	2489
BstBAI	YACGTR	1 cut(s)	59
BstDEI	CTNAG	7 cut(s)	873, 1638, 1893, 2006, 2511, 2526, 2760
BstDSI	CCRYGG	2 cut(s)	1089, 1455
BstEII	GGTNACC	1 cut(s)	2261
BstF5I	GGATG	7 cut(s)	240, 332, 817, 1478, 1574, 1753, 2167
BstFNI	CGCG	1 cut(s)	2778
BstH2I	RGCGCY	1 cut(s)	94
BstHHI	GCGC	2 cut(s)	93, 817
BstMAI	GTCTC	4 cut(s)	68, 1941, 2498, 2591
BstMWI	GCNNNNNNNGC	6 cut(s)	1541, 1618, 2183, 2213, 2408, 2747
BstNI	CCWGG	6 cut(s)	524, 606, 830, 1736, 1745, 2649
BstNSI	RCATGY	1 cut(s)	2437
BstPAI	GACNNNNGTC	1 cut(s)	2231
BstPI	GGTNACC	1 cut(s)	2261
BstSCI	CCNGG	7 cut(s)	522, 604, 807, 828, 1734, 1743, 2647
BstSFI	CTRYAG	3 cut(s)	325, 636, 993
BstSLI	GKGCMC	3 cut(s)	917, 1110, 2354
BstUI	CGCG	1 cut(s)	2778
BstV1I	GCAGC	8 cut(s)	98, 804, 1553, 1599, 1676, 2065, 2123, 2725
BstV2I	GAAGAC	2 cut(s)	1595, 2147
BstX2I	RGATCY	6 cut(s)	238, 245, 1147, 1526, 1740, 1862
BstXI	CCANNNNNNTGG	2 cut(s)	2021, 2648
BstYI	RGATCY	6 cut(s)	238, 245, 1147, 1526, 1740, 1862
Bsu36I	CCTNAGG	1 cut(s)	2006
BsuI	GTATCC	2 cut(s)	727, 2663
BsuRI	GGCC	7 cut(s)	97, 564, 609, 915, 1994, 2196, 2545
BtgI	CCRYGG	2 cut(s)	1089, 1455
BtgZI	GCGATG	1 cut(s)	2426
BtrI	CACGTC	1 cut(s)	967
BtsCI	GGATG	7 cut(s)	240, 332, 817, 1478, 1574, 1753, 2167
BtsI	GCAGTG	1 cut(s)	1002
BtsIMutI	CAGTG	4 cut(s)	1002, 1357, 2103, 2692
BveI	ACCTGC	4 cut(s)	1325, 2040, 2198, 2380
CciI	TCATGA	1 cut(s)	318
CfoI	GCGC	2 cut(s)	93, 817
Cfr10I	RCCGGY	2 cut(s)	564, 897
Cfr13I	GGNCC	8 cut(s)	96, 574, 913, 914, 1358, 2000, 2210, 2452
CsiI	ACCWGGT	1 cut(s)	2647
Csp6I	GTAC	8 cut(s)	201, 443, 656, 973, 1175, 1338, 1627, 2238
CspAI	ACCGGT	1 cut(s)	897
CviQI	GTAC	8 cut(s)	201, 443, 656, 973, 1175, 1338, 1627, 2238
DdeI	CTNAG	7 cut(s)	873, 1638, 1893, 2006, 2511, 2526, 2760
DraI	TTTAAA	1 cut(s)	2367
DraIII	CACNNNGTG	1 cut(s)	1933
DrdI	GACNNNNNNGTC	1 cut(s)	1654
DseDI	GACNNNNNNGTC	1 cut(s)	1654
EaeI	YGGCCR	2 cut(s)	607, 1992
Eam1104I	CTCTTC	2 cut(s)	1273, 2589
EarI	CTCTTC	2 cut(s)	1273, 2589
EciI	GGCGGA	3 cut(s)	566, 1023, 1907
Eco130I	CCWWGG	5 cut(s)	400, 943, 1089, 2381, 2665
Eco24I	GRGCYC	2 cut(s)	917, 2091
Eco47I	GGWCC	5 cut(s)	574, 1358, 2000, 2210, 2452
Eco57I	CTGAAG	3 cut(s)	47, 1104, 2570
Eco81I	CCTNAGG	1 cut(s)	2006
Eco88I	CYCGRG	2 cut(s)	513, 2591
Eco91I	GGTNACC	1 cut(s)	2261
EcoO109I	RGGNCCY	2 cut(s)	913, 914
EcoO65I	GGTNACC	1 cut(s)	2261
EcoRI	GAATTC	1 cut(s)	1883
EcoRII	CCWGG	6 cut(s)	522, 604, 828, 1734, 1743, 2647
EcoT14I	CCWWGG	5 cut(s)	400, 943, 1089, 2381, 2665
EcoT38I	GRGCYC	2 cut(s)	917, 2091
ErhI	CCWWGG	5 cut(s)	400, 943, 1089, 2381, 2665
FalI	AAGNNNNNCTT	4 cut(s)	187, 219, 2347, 2379
FaqI	GGGAC	2 cut(s)	1843, 2721
FauI	CCCGC	2 cut(s)	1586, 2458
FauNDI	CATATG	1 cut(s)	835
FblI	GTMKAC	1 cut(s)	1658
Fnu4HI	GCNGC	8 cut(s)	112, 818, 1567, 1613, 1690, 2079, 2112, 2739
FokI	GGATG	7 cut(s)	247, 319, 824, 1465, 1561, 1760, 2174
FriOI	GRGCYC	2 cut(s)	917, 2091
Fsp4HI	GCNGC	8 cut(s)	112, 818, 1567, 1613, 1690, 2079, 2112, 2739
FspBI	CTAG	5 cut(s)	1473, 1596, 1686, 2219, 2438
GlaI	GCGC	2 cut(s)	92, 816
GluI	GCNGC	8 cut(s)	112, 818, 1567, 1613, 1690, 2079, 2112, 2739
HaeII	RGCGCY	1 cut(s)	94
HaeIII	GGCC	7 cut(s)	97, 564, 609, 915, 1994, 2196, 2545
HapII	CCGG	4 cut(s)	565, 809, 898, 2715
HhaI	GCGC	2 cut(s)	93, 817
Hin6I	GCGC	2 cut(s)	91, 815
HinP1I	GCGC	2 cut(s)	91, 815
HincII	GTYRAC	4 cut(s)	73, 2395, 2539, 2701
HindII	GTYRAC	4 cut(s)	73, 2395, 2539, 2701
HindIII	AAGCTT	1 cut(s)	29
HpaII	CCGG	4 cut(s)	565, 809, 898, 2715
HphI	GGTGA	4 cut(s)	277, 1021, 1292, 2120
HpyAV	CCTTC	8 cut(s)	501, 554, 768, 893, 1765, 2280, 2422, 2464
HpyCH4III	ACNGT	9 cut(s)	265, 637, 692, 892, 989, 1042, 1144, 1952, 2687
HpyCH4IV	ACGT	3 cut(s)	58, 966, 1104
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1541, 1618, 2183, 2213, 2408, 2747
HpyF3I	CTNAG	7 cut(s)	873, 1638, 1893, 2006, 2511, 2526, 2760
HpySE526I	ACGT	3 cut(s)	58, 966, 1104
HspAI	GCGC	2 cut(s)	91, 815
KroI	GCCGGC	1 cut(s)	564
KroNI	GCCGGC	1 cut(s)	566
LguI	GCTCTTC	1 cut(s)	2589
LmnI	GCTCC	7 cut(s)	825, 859, 1434, 1920, 2010, 2094, 2386
Lsp1109I	GCAGC	8 cut(s)	98, 804, 1553, 1599, 1676, 2065, 2123, 2725
LweI	GCATC	4 cut(s)	802, 1321, 1583, 1738
MabI	ACCWGGT	1 cut(s)	2647
MaeI	CTAG	5 cut(s)	1473, 1596, 1686, 2219, 2438
MaeII	ACGT	3 cut(s)	58, 966, 1104
MaeIII	GTNAC	8 cut(s)	4, 280, 391, 640, 724, 1042, 1280, 2261
MfeI	CAATTG	1 cut(s)	789
MflI	RGATCY	6 cut(s)	238, 245, 1147, 1526, 1740, 1862
MhlI	GDGCHC	4 cut(s)	917, 1110, 2091, 2354
MlsI	TGGCCA	2 cut(s)	609, 1994
MluNI	TGGCCA	2 cut(s)	609, 1994
MlyI	GAGTC	2 cut(s)	736, 1264
MmeI	TCCRAC	3 cut(s)	157, 1341, 1383
Mox20I	TGGCCA	2 cut(s)	609, 1994
MroNI	GCCGGC	1 cut(s)	564
MroXI	GAANNNNTTC	2 cut(s)	668, 1971
MscI	TGGCCA	2 cut(s)	609, 1994
MslI	CAYNNNNRTG	5 cut(s)	1182, 2019, 2044, 2106, 2133
Msp20I	TGGCCA	2 cut(s)	609, 1994
MspA1I	CMGCKG	4 cut(s)	1082, 1373, 2243, 2510
MspCI	CTTAAG	1 cut(s)	2489
MspI	CCGG	4 cut(s)	565, 809, 898, 2715
MspR9I	CCNGG	7 cut(s)	524, 606, 809, 830, 1736, 1745, 2649
MunI	CAATTG	1 cut(s)	789
MvaI	CCWGG	6 cut(s)	524, 606, 830, 1736, 1745, 2649
MvnI	CGCG	1 cut(s)	2778
MwoI	GCNNNNNNNGC	6 cut(s)	1541, 1618, 2183, 2213, 2408, 2747
NaeI	GCCGGC	1 cut(s)	566
NciI	CCSGG	1 cut(s)	809
NcoI	CCATGG	1 cut(s)	1089
NdeI	CATATG	1 cut(s)	835
NgoMIV	GCCGGC	1 cut(s)	564
NheI	GCTAGC	1 cut(s)	1685
NlaIV	GGNNCC	8 cut(s)	575, 914, 915, 2002, 2012, 2090, 2454, 2619
NmuCI	GTSAC	3 cut(s)	391, 1042, 1280
NspI	RCATGY	1 cut(s)	2437
OliI	CACNNNNGTG	2 cut(s)	1182, 2106
PaeI	GCATGC	1 cut(s)	2437
PaeR7I	CTCGAG	2 cut(s)	513, 2591
PagI	TCATGA	1 cut(s)	318
PciSI	GCTCTTC	1 cut(s)	2589
PdiI	GCCGGC	1 cut(s)	566
PdmI	GAANNNNTTC	2 cut(s)	668, 1971
PfeI	GAWTC	8 cut(s)	53, 620, 844, 1364, 1462, 1503, 1972, 2569
PflMI	CCANNNNNTGG	2 cut(s)	616, 2449
PfoI	TCCNGGA	3 cut(s)	807, 1734, 1743
PinAI	ACCGGT	1 cut(s)	897
PkrI	GCNGC	8 cut(s)	113, 819, 1568, 1614, 1691, 2080, 2113, 2740
PleI	GAGTC	2 cut(s)	736, 1263
PpsI	GAGTC	2 cut(s)	736, 1263
Ppu21I	YACGTR	1 cut(s)	59
PshAI	GACNNNNGTC	1 cut(s)	2231
PshBI	ATTAAT	1 cut(s)	720
Psp6I	CCWGG	6 cut(s)	522, 604, 828, 1734, 1743, 2647
PspEI	GGTNACC	1 cut(s)	2261
PspGI	CCWGG	6 cut(s)	522, 604, 828, 1734, 1743, 2647
PspN4I	GGNNCC	8 cut(s)	575, 914, 915, 2002, 2012, 2090, 2454, 2619
PspOMI	GGGCCC	1 cut(s)	913
PspPI	GGNCC	8 cut(s)	96, 574, 913, 914, 1358, 2000, 2210, 2452
PspXI	VCTCGAGB	2 cut(s)	513, 2591
PstI	CTGCAG	1 cut(s)	997
PsuI	RGATCY	6 cut(s)	238, 245, 1147, 1526, 1740, 1862
PvuII	CAGCTG	4 cut(s)	1082, 1373, 2243, 2510
RsaI	GTAC	8 cut(s)	202, 444, 657, 974, 1176, 1339, 1628, 2239
RsaNI	GTAC	8 cut(s)	201, 443, 656, 973, 1175, 1338, 1627, 2238
RseI	CAYNNNNRTG	5 cut(s)	1182, 2019, 2044, 2106, 2133
SapI	GCTCTTC	1 cut(s)	2589
SatI	GCNGC	8 cut(s)	112, 818, 1567, 1613, 1690, 2079, 2112, 2739
Sau96I	GGNCC	8 cut(s)	96, 574, 913, 914, 1358, 2000, 2210, 2452
SchI	GAGTC	2 cut(s)	736, 1264
ScrFI	CCNGG	7 cut(s)	524, 606, 809, 830, 1736, 1745, 2649
SduI	GDGCHC	4 cut(s)	917, 1110, 2091, 2354
SexAI	ACCWGGT	1 cut(s)	2647
SfaNI	GCATC	4 cut(s)	802, 1321, 1583, 1738
SfcI	CTRYAG	3 cut(s)	325, 636, 993
Sfr274I	CTCGAG	2 cut(s)	513, 2591
SinI	GGWCC	5 cut(s)	574, 1358, 2000, 2210, 2452
SlaI	CTCGAG	2 cut(s)	513, 2591
SmiMI	CAYNNNNRTG	5 cut(s)	1182, 2019, 2044, 2106, 2133
SmlI	CTYRAG	3 cut(s)	513, 2489, 2591
SmoI	CTYRAG	3 cut(s)	513, 2489, 2591
SphI	GCATGC	1 cut(s)	2437
SspMI	CTAG	5 cut(s)	1473, 1596, 1686, 2219, 2438
StyD4I	CCNGG	7 cut(s)	522, 604, 807, 828, 1734, 1743, 2647
StyI	CCWWGG	5 cut(s)	400, 943, 1089, 2381, 2665
TaaI	ACNGT	9 cut(s)	265, 637, 692, 892, 989, 1042, 1144, 1952, 2687
TaiI	ACGT	3 cut(s)	61, 969, 1107
TaqI	TCGA	6 cut(s)	354, 406, 514, 1271, 1764, 2592
TatI	WGTACW	3 cut(s)	442, 655, 2237
TfiI	GAWTC	8 cut(s)	53, 620, 844, 1364, 1462, 1503, 1972, 2569
TscAI	CASTG	4 cut(s)	1002, 1357, 2110, 2692
TseFI	GTSAC	3 cut(s)	391, 1042, 1280
TseI	GCWGC	8 cut(s)	111, 817, 1566, 1612, 1689, 2078, 2111, 2738
Tsp45I	GTSAC	3 cut(s)	391, 1042, 1280
TspGWI	ACGGA	3 cut(s)	1444, 1644, 2315
TspRI	CASTG	4 cut(s)	1002, 1357, 2110, 2692
Van91I	CCANNNNNTGG	2 cut(s)	616, 2449
Vha464I	CTTAAG	1 cut(s)	2489
VneI	GTGCAC	1 cut(s)	2350
VpaK11BI	GGWCC	5 cut(s)	574, 1358, 2000, 2210, 2452
VspI	ATTAAT	1 cut(s)	720
XapI	RAATTY	8 cut(s)	457, 542, 649, 1153, 1234, 1633, 1883, 2064
XbaI	TCTAGA	1 cut(s)	2218
XceI	RCATGY	1 cut(s)	2437
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2043, 2155
XhoI	CTCGAG	2 cut(s)	513, 2591
XmiI	GTMKAC	1 cut(s)	1658
XmnI	GAANNNNTTC	2 cut(s)	668, 1971
XspI	CTAG	5 cut(s)	1473, 1596, 1686, 2219, 2438

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.