Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0004410

Physical Location & Seq

Genomic Coordinates Reverse (-)

24880742 .. 24884411

Gene Structure 3670 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000013014

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2535 bp

ATGTTGAAGATGAAACTAGTCAGTTGGGTACCTTTGGTTCTGGCTGTTGTTGTGTTTATGAGTCATGGAGCCGGTGGTTTATTCAATCCTGTTGATCACTACTTGATCGCTTGTGGTTCTTCGAAAAATGTCACATTTCAAGGCCGAACCTTTGTTCCTGATACACTGCAACCTTCCCTTATACTGAAAAGTGCAAATTCTTTAGTTGCTAGCTCCAGTTCCAATGCCCCATCTCCTATTTACCAATCTGCTCGAATTTTCAAGGAAACATCTTCCTATAAATTCAAAATCCAGAAAGAAGGCCGGCATTGGGTCCGGCTTCATTTCTACCCTTTAGCAAATTCGGGCCAAAACTTGACGTCTGCTCTGATAACTGTGGTCACCGATAGCTTTGTACTCTTGAACAACTTCACTTTTGAGAACTATACTGGTTCTTATCTGTTCAAGGAGTATGCAATCAATGTAACTTCAGATAGTTTGACCCTCACCATCATTCCTTCAAACAGTTCAGTTGCTTTTGTTAATGCAATTGAAGTTGTCTCGATCCCGGATGAGCTAATCCCTGATCAGGCATATGCTATAAATCCATCTGCTCCTTTTAGTGGTCTTTCTGCACTTGCCCTTCAAACAATGTACCGATTAAACATGGGGGGTCCTTTGCTCACTGCTGAGAATGATACCCTTAAAAGAACTTGGGAGAATGATTTGAAATACCTTCATGTCGACAGCTCTGCAGTGAATGTATCAATCAACCCTGCCAGCATAAAGTATCCGGTCACTGTCACACCAGAAATAGCACCAAATTGGGTCTATGCCACTGCTGAAGCCATGGGAGATGCGAATGTGCCAAATGGAAACTTCAACATAACTTGGGTCTTTCCAGCAGATCCAAATTATTTGTATCTTGTTCGGGTACATTTCTGTGATATTGTCAGCAAGGCACTGAATAACCTTGTTTTCAATCTTTTCATCAATTCCGACAATGTGCTCGCGAGTCTTGACCTCTCTTCCATTACTGGTGACTTGAATGTGCCGTATTACAAAGACTTTGTTTCCAACTCCTCAGCTGATTCAAATACTTTGACAGTCAGTGTAGGTCCAGATACAATGGCTGATGTCACTAATGCAATTCTGAATGGCTTGGAGATTTTGAAGATCAGCAATGACTTGGGGAGCTTGGACGGGTCTTTGTCTGTTCAGAGTCTCCTTCCCAGTTCACCCTCGAAGAGCAATAAGATACTAATCTTAGTTGGGTGTGTTGTAGGAGCTGTAGCTCTTGTGGGAATAATCGCTTTCTTTTATTGCTGCCTGGCATCTCGCCAGTCCAAGACTACTCCTAACCAAGGGCATCCATGGCTGCTTCTGCCCTTGTATGGAAGCTCTCAGACAATGACCAAAATGTCCACAACTTCACAGAAGAGTGCAACAGCTAGCTGCATTTCATTAGTTTCCTCCAATCTTGGCCGCTTATTCATGTTCCAAGAAATCCTAGATGCAACCAACAAGTTTGATGAGAGCCTACTTCTCGGGGTGGGCGGTTTTGGCAGAGTTTACAAGGGAACACTAGAAGATGGGACAAAAGTAGCTGTTAAAAGAGGAAACCCCAGATCCGAGCAAGGTCTTGCTGAATTCCGAACTGAGATTGAAATGTTGTCTAAGCTTCGTCATCGCCACCTTGTGTCTCTTATTGGTTACTGTGATGAAAGGTCTGAGATGATTCTTGTGTATGAATACATGGCTAATGGACCGCTCCGGAGCCATTTGTATGGAACAGATTTGCCTACCCTATCATGGAAGCTACGCCTTGAAATTTGCATCGGGGCTGCAAGAGGGCTCCATTATCTTCACACTGGTGCAGCTCAAAGCATAATTCACCGAGACGTGAAGACAACAAACATTCTTTTGGATGAGAACTTTGTAGCCAAAGTTGCAGATTTTGGCCTTTCAAAAGCAGGTCCAGCTATAGATCAGACTCATGTCAGTACAGCTGTTAAGGGTAGTTTTGGTTACCTTGATCCTGAATACTTTAGGAGGCAGCAGCTCACTGAGAAATCAGATGTGTATTCGTTTGGTGTAGTTCTAATGGAAGTTCTTTGCACAAGACCTGCTTTGAACCCGGTTCTTCCCAGAGAGCAAGTGAACATAGCAGAATGGGCAATGACCTGGCAAAAGAAGGGCATGCTGGACCAAATCATGGACCCGAATCTGGCTGGGAAGGTAAATCCAGCTTCTCTTAAGAAGTTTGGTGAGACAGCTGAGAAGTGCCTTGCCGAGTATGGTGTTGATAGGCCATCAATGGGAGATGTCTTGTGGAATCTTGAATATGCTCTTCAGCTTGAGGAGACTTCATCTGCACTCATGGAACCTGAAGATAACAGCACGAACCACATACGAGATATTGAATTGACACCACTTGAGCCATTTGACAACAGTGTGAGTATGATCGAGGGAGGGAACTCTGGCACAGATGACGATGCTGAAGATGCTGCCACAAGTGCTGTGTTCTCTCAGCTAGTCAATCCTCGTGGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0009814 GO:0009817 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0036211 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Malectin_like Pkinase_Tyr

Protein Analysis

845

Amino Acids

92.33

Weight (kDa)

5.39

Isoelectric Point (pI)

39.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin_like	PF12819	36 - 385	4.4e-30	Malectin-like domain
Pkinase	PF00069	508 - 766	5.5e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	509 - 700	3.1e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004410)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54380
fragaria_vesca	FvH4_2g16820 FvH4_2g16820 FvH4_3g20780 FvH4_3g20780 FvH4_3g20780
malus_domestica	MD03G1221300.v1.1 MD11G1238700.v1.1
prunus_persica	Prupe.4G188000_v2.0.a1
pyrus_communis	pycom03g17020 pycom11g21030
rosa_chinensis	RchiOBHm_Chr5g0035071 RchiOBHm_Chr6g0280941
rosa_laevigata	RLG00000013014 RLG00000033592
rosa_multiflora	Rmu_sc0005362.1_g000028 Rmu_sc0023551.1_g000001 Rmu_sc0023552.1_g000001
rosa_roxburghii	Rroxscaffold_1G00045340 Rroxscaffold_7G00187470
rosa_rugosa	Rorug05G0149600 Rorug06G0137100
rosa_samantha	Rh5AG242600 Rh5BG242200 Rh5CG273700 Rh5DG250800 Rh6AG249400 Rh6BG251500 Rh6CG252100 Rh6DG243900
rosa_wichuraiana	Rw5G022160 Rw6G021580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1399
AatII	GACGTC	1 cut(s)	362
Acc36I	ACCTGC	2 cut(s)	1943, 2113
Acc65I	GGTACC	1 cut(s)	28
AccB1I	GGYRCC	1 cut(s)	28
AccB7I	CCANNNNNTGG	1 cut(s)	2194
AccBSI	CCGCTC	1 cut(s)	1750
AccI	GTMKAC	1 cut(s)	723
AccII	CGCG	1 cut(s)	992
AccIII	TCCGGA	1 cut(s)	1752
AciI	CCGC	3 cut(s)	1465, 1536, 1748
AclWI	GGATC	4 cut(s)	538, 881, 1602, 2009
AcoI	YGGCCR	1 cut(s)	1462
AcsI	RAATTY	6 cut(s)	196, 255, 281, 340, 1628, 1809
AcuI	CTGAAG	5 cut(s)	453, 843, 2315, 2388, 2499
AcyI	GRCGYC	1 cut(s)	359
AdeI	CACNNNGTG	1 cut(s)	1678
AfaI	GTAC	5 cut(s)	30, 396, 635, 915, 1984
AfiI	CCNNNNNNNGG	9 cut(s)	310, 568, 602, 1343, 1373, 1791, 2194, 2206, 2297
AflII	CTTAAG	1 cut(s)	2234
AhlI	ACTAGT	1 cut(s)	16
AjiI	CACGTC	1 cut(s)	1882
AjnI	CCWGG	2 cut(s)	1308, 2162
AleI	CACNNNNGTG	1 cut(s)	1851
AloI	GAACNNNNNNTCC	2 cut(s)	139, 171
Alw21I	GWGCWC	1 cut(s)	990
Alw26I	GTCTC	6 cut(s)	544, 1208, 1686, 1872, 2243, 2336
AlwI	GGATC	4 cut(s)	538, 881, 1602, 2009
Ama87I	CYCGRG	1 cut(s)	1526
Aor13HI	TCCGGA	1 cut(s)	1752
AoxI	GGCC	6 cut(s)	142, 301, 346, 1462, 1939, 2288
ApeKI	GCWGC	8 cut(s)	1305, 1357, 1434, 1823, 1856, 2035, 2038, 2486
ApoI	RAATTY	6 cut(s)	196, 255, 281, 340, 1628, 1809
Asp700I	GAANNNNTTC	1 cut(s)	407
Asp718I	GGTACC	1 cut(s)	28
AspS9I	GGNCC	8 cut(s)	313, 346, 653, 1097, 1745, 1955, 2185, 2197
AsuC2I	CCSGG	2 cut(s)	548, 2117
AsuHPI	GGTGA	6 cut(s)	373, 478, 1031, 1209, 1865, 2258
AsuII	TTCGAA	1 cut(s)	122
AsuNHI	GCTAGC	2 cut(s)	209, 1430
AvaI	CYCGRG	1 cut(s)	1526
AvaII	GGWCC	7 cut(s)	313, 653, 1097, 1745, 1955, 2185, 2197
BanI	GGYRCC	1 cut(s)	28
BanII	GRGCYC	1 cut(s)	1836
BauI	CACGAG	1 cut(s)	2523
BbsI	GAAGAC	1 cut(s)	1892
Bbv12I	GWGCWC	1 cut(s)	990
BbvCI	CCTCAGC	1 cut(s)	1063
BbvI	GCAGC	8 cut(s)	1292, 1344, 1421, 1810, 1868, 2047, 2050, 2473
BccI	CCATC	5 cut(s)	238, 497, 595, 1565, 2299
BceAI	ACGGC	1 cut(s)	1018
BcgI	CGANNNNNNTGC	2 cut(s)	713, 747
BciT130I	CCWGG	2 cut(s)	1310, 2164
BciVI	GTATCC	1 cut(s)	780
BclI	TGATCA	2 cut(s)	94, 565
BcnI	CCSGG	2 cut(s)	548, 2117
BcoDI	GTCTC	6 cut(s)	544, 1208, 1686, 1872, 2243, 2336
BcuI	ACTAGT	1 cut(s)	16
BfaI	CTAG	6 cut(s)	17, 210, 1431, 1490, 1565, 2513
BfmI	CTRYAG	3 cut(s)	732, 1269, 1962
BfrI	CTTAAG	1 cut(s)	2234
BfuAI	ACCTGC	2 cut(s)	1943, 2113
BfuI	GTATCC	1 cut(s)	780
BisI	GCNGC	9 cut(s)	1306, 1358, 1435, 1465, 1824, 1857, 2036, 2039, 2487
BlsI	GCNGC	9 cut(s)	1307, 1359, 1436, 1466, 1825, 1858, 2037, 2040, 2488
Bme1390I	CCNGG	4 cut(s)	548, 1310, 2117, 2164
Bme18I	GGWCC	7 cut(s)	313, 653, 1097, 1745, 1955, 2185, 2197
BmeT110I	CYCGRG	1 cut(s)	1526
BmgBI	CACGTC	1 cut(s)	1882
BmgT120I	GGNCC	8 cut(s)	313, 346, 653, 1097, 1745, 1955, 2185, 2197
BmiI	GGNNCC	8 cut(s)	30, 70, 314, 654, 1757, 1835, 2199, 2364
BmrFI	CCNGG	4 cut(s)	548, 1310, 2117, 2164
BmrI	ACTGGG	1 cut(s)	1206
BmsI	GCATC	7 cut(s)	826, 1322, 1357, 1483, 1824, 2464, 2473
BmtI	GCTAGC	2 cut(s)	213, 1434
BmuI	ACTGGG	1 cut(s)	1206
BoxI	GACNNNNGTC	1 cut(s)	1976
BpiI	GAAGAC	1 cut(s)	1892
BpmI	CTGGAG	1 cut(s)	199
Bpu10I	CCTNAGC	1 cut(s)	1063
Bpu14I	TTCGAA	1 cut(s)	122
BpuEI	CTTGAG	2 cut(s)	2357, 2435
BpuMI	CCSGG	2 cut(s)	548, 2117
BsaBI	GATNNNNATC	1 cut(s)	1241
BsaHI	GRCGYC	1 cut(s)	359
BsaJI	CCNNGG	3 cut(s)	828, 1342, 1352
BsaWI	WCCGGW	2 cut(s)	772, 1752
Bsc4I	CCNNNNNNNGG	9 cut(s)	310, 568, 602, 1343, 1373, 1791, 2194, 2206, 2297
Bse118I	RCCGGY	2 cut(s)	71, 303
Bse1I	ACTGG	6 cut(s)	216, 433, 1021, 1212, 1321, 1855
Bse3DI	GCAATG	2 cut(s)	1168, 2163
Bse8I	GATNNNNATC	1 cut(s)	1241
BseAI	TCCGGA	1 cut(s)	1752
BseBI	CCWGG	2 cut(s)	1310, 2164
BseDI	CCNNGG	3 cut(s)	828, 1342, 1352
BseGI	GGATG	3 cut(s)	556, 1348, 1912
BseJI	GATNNNNATC	1 cut(s)	1241
BseLI	CCNNNNNNNGG	9 cut(s)	310, 568, 602, 1343, 1373, 1791, 2194, 2206, 2297
BseMI	GCAATG	2 cut(s)	1168, 2163
BseMII	CTCAG	8 cut(s)	660, 1077, 1397, 1629, 1701, 2037, 2247, 2522
BseNI	ACTGG	6 cut(s)	216, 433, 1021, 1212, 1321, 1855
BseRI	GAGGAG	2 cut(s)	1051, 2354
BseXI	GCAGC	8 cut(s)	1292, 1344, 1421, 1810, 1868, 2047, 2050, 2473
BseYI	CCCAGC	1 cut(s)	2210
BsgI	GTGCAG	3 cut(s)	597, 1875, 2337
Bsh1236I	CGCG	1 cut(s)	992
BshFI	GGCC	6 cut(s)	144, 303, 348, 1464, 1941, 2290
BshNI	GGYRCC	1 cut(s)	28
BsiHKAI	GWGCWC	1 cut(s)	990
BsiHKCI	CYCGRG	1 cut(s)	1526
BsiSI	CCGG	7 cut(s)	72, 304, 316, 548, 773, 1753, 2117
BslFI	GGGAC	1 cut(s)	1588
BslI	CCNNNNNNNGG	9 cut(s)	310, 568, 602, 1343, 1373, 1791, 2194, 2206, 2297
BsmAI	GTCTC	6 cut(s)	544, 1208, 1686, 1872, 2243, 2336
BsmBI	CGTCTC	1 cut(s)	1872
BsmFI	GGGAC	1 cut(s)	1588
BsnI	GGCC	6 cut(s)	144, 303, 348, 1464, 1941, 2290
BsoBI	CYCGRG	1 cut(s)	1526
Bsp119I	TTCGAA	1 cut(s)	122
Bsp1286I	GDGCHC	2 cut(s)	990, 1836
Bsp13I	TCCGGA	1 cut(s)	1752
Bsp19I	CCATGG	2 cut(s)	828, 1352
Bsp68I	TCGCGA	1 cut(s)	992
BspACI	CCGC	3 cut(s)	1465, 1536, 1748
BspANI	GGCC	6 cut(s)	144, 303, 348, 1464, 1941, 2290
BspCNI	CTCAG	8 cut(s)	661, 1076, 1396, 1630, 1702, 2038, 2248, 2521
BspEI	TCCGGA	1 cut(s)	1752
BspFNI	CGCG	1 cut(s)	992
BspLI	GGNNCC	8 cut(s)	30, 70, 314, 654, 1757, 1835, 2199, 2364
BspMAI	CTGCAG	1 cut(s)	736
BspMI	ACCTGC	2 cut(s)	1943, 2113
BspOI	GCTAGC	2 cut(s)	213, 1434
BspPI	GGATC	4 cut(s)	538, 881, 1602, 2009
BspQI	GCTCTTC	2 cut(s)	1220, 2334
BspT104I	TTCGAA	1 cut(s)	122
BspT107I	GGYRCC	1 cut(s)	28
BspTI	CTTAAG	1 cut(s)	2234
BsrBI	CCGCTC	1 cut(s)	1750
BsrDI	GCAATG	2 cut(s)	1168, 2163
BsrFI	RCCGGY	2 cut(s)	71, 303
BsrI	ACTGG	6 cut(s)	216, 433, 1021, 1212, 1321, 1855
BssAI	RCCGGY	2 cut(s)	71, 303
BssECI	CCNNGG	3 cut(s)	828, 1342, 1352
BssNI	GRCGYC	1 cut(s)	359
BssSI	CACGAG	1 cut(s)	2523
BssT1I	CCWWGG	3 cut(s)	828, 1342, 1352
Bst2BI	CACGAG	1 cut(s)	2523
Bst2UI	CCWGG	2 cut(s)	1310, 2164
Bst4CI	ACNGT	6 cut(s)	376, 506, 781, 1087, 1697, 2432
Bst6I	CTCTTC	4 cut(s)	1012, 1220, 1412, 2334
BstACI	GRCGYC	1 cut(s)	359
BstAFI	CTTAAG	1 cut(s)	2234
BstBI	TTCGAA	1 cut(s)	122
BstC8I	GCNNGC	6 cut(s)	211, 305, 760, 990, 1432, 2180
BstDSI	CCRYGG	2 cut(s)	828, 1352
BstEII	GGTNACC	2 cut(s)	379, 2006
BstENI	CCTNNNNNAGG	1 cut(s)	1341
BstF5I	GGATG	3 cut(s)	556, 1348, 1912
BstFNI	CGCG	1 cut(s)	992
BstMAI	GTCTC	6 cut(s)	544, 1208, 1686, 1872, 2243, 2336
BstMWI	GCNNNNNNNGC	8 cut(s)	1354, 1363, 1542, 1928, 1958, 2153, 2483, 2495
BstNI	CCWGG	2 cut(s)	1310, 2164
BstNSI	RCATGY	1 cut(s)	2182
BstPAI	GACNNNNGTC	1 cut(s)	1976
BstPI	GGTNACC	2 cut(s)	379, 2006
BstSCI	CCNGG	4 cut(s)	546, 1308, 2115, 2162
BstSFI	CTRYAG	3 cut(s)	732, 1269, 1962
BstUI	CGCG	1 cut(s)	992
BstV1I	GCAGC	8 cut(s)	1292, 1344, 1421, 1810, 1868, 2047, 2050, 2473
BstV2I	GAAGAC	1 cut(s)	1892
BstX2I	RGATCY	2 cut(s)	886, 1607
BstXI	CCANNNNNNTGG	1 cut(s)	1766
BstYI	RGATCY	2 cut(s)	886, 1607
BsuI	GTATCC	1 cut(s)	780
BsuRI	GGCC	6 cut(s)	144, 303, 348, 1464, 1941, 2290
BtgI	CCRYGG	2 cut(s)	828, 1352
BtgZI	GCGATG	1 cut(s)	1652
BtrI	CACGTC	1 cut(s)	1882
BtsCI	GGATG	3 cut(s)	556, 1348, 1912
BtsI	GCAGTG	4 cut(s)	164, 663, 741, 816
BtuMI	TCGCGA	1 cut(s)	992
BveI	ACCTGC	2 cut(s)	1943, 2113
Cac8I	GCNNGC	6 cut(s)	211, 305, 760, 990, 1432, 2180
Cfr10I	RCCGGY	2 cut(s)	71, 303
Cfr13I	GGNCC	8 cut(s)	313, 346, 653, 1097, 1745, 1955, 2185, 2197
Csp6I	GTAC	5 cut(s)	29, 395, 634, 914, 1983
CspCI	CAANNNNNGTGG	1 cut(s)	2506
CviQI	GTAC	5 cut(s)	29, 395, 634, 914, 1983
DraIII	CACNNNGTG	1 cut(s)	1678
DrdI	GACNNNNNNGTC	1 cut(s)	1399
DseDI	GACNNNNNNGTC	1 cut(s)	1399
EaeI	YGGCCR	1 cut(s)	1462
Eam1104I	CTCTTC	4 cut(s)	1012, 1220, 1412, 2334
EarI	CTCTTC	4 cut(s)	1012, 1220, 1412, 2334
Eco130I	CCWWGG	3 cut(s)	828, 1342, 1352
Eco24I	GRGCYC	1 cut(s)	1836
Eco47I	GGWCC	7 cut(s)	313, 653, 1097, 1745, 1955, 2185, 2197
Eco57I	CTGAAG	5 cut(s)	453, 843, 2315, 2388, 2499
Eco88I	CYCGRG	1 cut(s)	1526
Eco91I	GGTNACC	2 cut(s)	379, 2006
EcoNI	CCTNNNNNAGG	1 cut(s)	1341
EcoO109I	RGGNCCY	1 cut(s)	653
EcoO65I	GGTNACC	2 cut(s)	379, 2006
EcoRI	GAATTC	1 cut(s)	1628
EcoRII	CCWGG	2 cut(s)	1308, 2162
EcoT14I	CCWWGG	3 cut(s)	828, 1342, 1352
EcoT38I	GRGCYC	1 cut(s)	1836
ErhI	CCWWGG	3 cut(s)	828, 1342, 1352
Esp3I	CGTCTC	1 cut(s)	1872
FalI	AAGNNNNNCTT	2 cut(s)	2092, 2124
FaqI	GGGAC	1 cut(s)	1588
FauNDI	CATATG	1 cut(s)	574
FbaI	TGATCA	2 cut(s)	94, 565
FblI	GTMKAC	1 cut(s)	723
Fnu4HI	GCNGC	9 cut(s)	1306, 1358, 1435, 1465, 1824, 1857, 2036, 2039, 2487
FokI	GGATG	3 cut(s)	563, 1335, 1919
FriOI	GRGCYC	1 cut(s)	1836
Fsp4HI	GCNGC	9 cut(s)	1306, 1358, 1435, 1465, 1824, 1857, 2036, 2039, 2487
FspBI	CTAG	6 cut(s)	17, 210, 1431, 1490, 1565, 2513
GluI	GCNGC	9 cut(s)	1306, 1358, 1435, 1465, 1824, 1857, 2036, 2039, 2487
GsaI	CCCAGC	1 cut(s)	2214
GsuI	CTGGAG	1 cut(s)	199
HaeIII	GGCC	6 cut(s)	144, 303, 348, 1464, 1941, 2290
HapII	CCGG	7 cut(s)	72, 304, 316, 548, 773, 1753, 2117
Hin1I	GRCGYC	1 cut(s)	359
HincII	GTYRAC	1 cut(s)	724
HindII	GTYRAC	1 cut(s)	724
HindIII	AAGCTT	1 cut(s)	1658
HinfI	GANTC	8 cut(s)	61, 994, 1070, 1201, 1717, 1972, 2203, 2314
HpaII	CCGG	7 cut(s)	72, 304, 316, 548, 773, 1753, 2117
HphI	GGTGA	6 cut(s)	373, 478, 1031, 1209, 1865, 2258
Hpy166II	GTNNAC	5 cut(s)	724, 1217, 1404, 1552, 2140
Hpy8I	GTNNAC	5 cut(s)	724, 1217, 1404, 1552, 2140
HpyAV	CCTTC	8 cut(s)	183, 293, 507, 632, 725, 1217, 2167, 2209
HpyCH4III	ACNGT	6 cut(s)	376, 506, 781, 1087, 1697, 2432
HpyCH4IV	ACGT	2 cut(s)	359, 1881
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1354, 1363, 1542, 1928, 1958, 2153, 2483, 2495
HpySE526I	ACGT	2 cut(s)	359, 1881
Hsp92I	GRCGYC	1 cut(s)	359
Kpn2I	TCCGGA	1 cut(s)	1752
KpnI	GGTACC	1 cut(s)	32
KroI	GCCGGC	1 cut(s)	303
KroNI	GCCGGC	1 cut(s)	305
Ksp22I	TGATCA	2 cut(s)	94, 565
LguI	GCTCTTC	2 cut(s)	1220, 2334
LmnI	GCTCC	8 cut(s)	68, 218, 598, 1173, 1265, 1755, 1755, 1839
Lsp1109I	GCAGC	8 cut(s)	1292, 1344, 1421, 1810, 1868, 2047, 2050, 2473
LweI	GCATC	7 cut(s)	826, 1322, 1357, 1483, 1824, 2464, 2473
MaeI	CTAG	6 cut(s)	17, 210, 1431, 1490, 1565, 2513
MaeII	ACGT	2 cut(s)	359, 1881
MaeIII	GTNAC	9 cut(s)	130, 379, 463, 775, 781, 1019, 1117, 1691, 2006
MbiI	CCGCTC	1 cut(s)	1750
MfeI	CAATTG	1 cut(s)	528
MflI	RGATCY	2 cut(s)	886, 1607
MhlI	GDGCHC	2 cut(s)	990, 1836
MlyI	GAGTC	4 cut(s)	70, 1003, 1210, 1966
MmeI	TCCRAC	2 cut(s)	1002, 1080
MroI	TCCGGA	1 cut(s)	1752
MroNI	GCCGGC	1 cut(s)	303
MroXI	GAANNNNTTC	1 cut(s)	407
MseI	TTAA	6 cut(s)	522, 641, 684, 1590, 1992, 2235
MslI	CAYNNNNRTG	3 cut(s)	921, 1764, 1851
MspA1I	CMGCKG	3 cut(s)	1067, 1988, 2255
MspCI	CTTAAG	1 cut(s)	2234
MspI	CCGG	7 cut(s)	72, 304, 316, 548, 773, 1753, 2117
MspR9I	CCNGG	4 cut(s)	548, 1310, 2117, 2164
MunI	CAATTG	1 cut(s)	528
MvaI	CCWGG	2 cut(s)	1310, 2164
MvnI	CGCG	1 cut(s)	992
MwoI	GCNNNNNNNGC	8 cut(s)	1354, 1363, 1542, 1928, 1958, 2153, 2483, 2495
NaeI	GCCGGC	1 cut(s)	305
NciI	CCSGG	2 cut(s)	548, 2117
NcoI	CCATGG	2 cut(s)	828, 1352
NdeI	CATATG	1 cut(s)	574
NgoMIV	GCCGGC	1 cut(s)	303
NheI	GCTAGC	2 cut(s)	209, 1430
NlaIV	GGNNCC	8 cut(s)	30, 70, 314, 654, 1757, 1835, 2199, 2364
NmeAIII	GCCGAG	1 cut(s)	2296
NmuCI	GTSAC	6 cut(s)	130, 379, 775, 781, 1019, 1117
NruI	TCGCGA	1 cut(s)	992
NspI	RCATGY	1 cut(s)	2182
NspV	TTCGAA	1 cut(s)	122
OliI	CACNNNNGTG	1 cut(s)	1851
PaeI	GCATGC	1 cut(s)	2182
PciSI	GCTCTTC	2 cut(s)	1220, 2334
PdiI	GCCGGC	1 cut(s)	305
PdmI	GAANNNNTTC	1 cut(s)	407
PfeI	GAWTC	4 cut(s)	1070, 1717, 2203, 2314
PflMI	CCANNNNNTGG	1 cut(s)	2194
PfoI	TCCNGGA	1 cut(s)	546
PkrI	GCNGC	9 cut(s)	1307, 1359, 1436, 1466, 1825, 1858, 2037, 2040, 2488
PleI	GAGTC	4 cut(s)	69, 1002, 1209, 1966
PpsI	GAGTC	4 cut(s)	69, 1002, 1209, 1966
PpuMI	RGGWCCY	1 cut(s)	653
PshAI	GACNNNNGTC	1 cut(s)	1976
Psp5II	RGGWCCY	1 cut(s)	653
Psp6I	CCWGG	2 cut(s)	1308, 2162
PspEI	GGTNACC	2 cut(s)	379, 2006
PspFI	CCCAGC	1 cut(s)	2210
PspGI	CCWGG	2 cut(s)	1308, 2162
PspN4I	GGNNCC	8 cut(s)	30, 70, 314, 654, 1757, 1835, 2199, 2364
PspPI	GGNCC	8 cut(s)	313, 346, 653, 1097, 1745, 1955, 2185, 2197
PspPPI	RGGWCCY	1 cut(s)	653
PsrI	GAACNNNNNNTAC	2 cut(s)	21, 53
PstI	CTGCAG	1 cut(s)	736
PsuI	RGATCY	2 cut(s)	886, 1607
PvuII	CAGCTG	3 cut(s)	1067, 1988, 2255
RruI	TCGCGA	1 cut(s)	992
RsaI	GTAC	5 cut(s)	30, 396, 635, 915, 1984
RsaNI	GTAC	5 cut(s)	29, 395, 634, 914, 1983
RseI	CAYNNNNRTG	3 cut(s)	921, 1764, 1851
SalI	GTCGAC	1 cut(s)	722
SapI	GCTCTTC	2 cut(s)	1220, 2334
SaqAI	TTAA	6 cut(s)	522, 641, 684, 1590, 1992, 2235
SatI	GCNGC	9 cut(s)	1306, 1358, 1435, 1465, 1824, 1857, 2036, 2039, 2487
Sau96I	GGNCC	8 cut(s)	313, 346, 653, 1097, 1745, 1955, 2185, 2197
SchI	GAGTC	4 cut(s)	70, 1003, 1210, 1966
ScrFI	CCNGG	4 cut(s)	548, 1310, 2117, 2164
SduI	GDGCHC	2 cut(s)	990, 1836
SfaNI	GCATC	7 cut(s)	826, 1322, 1357, 1483, 1824, 2464, 2473
SfcI	CTRYAG	3 cut(s)	732, 1269, 1962
SfuI	TTCGAA	1 cut(s)	122
SinI	GGWCC	7 cut(s)	313, 653, 1097, 1745, 1955, 2185, 2197
SmiMI	CAYNNNNRTG	3 cut(s)	921, 1764, 1851
SmlI	CTYRAG	3 cut(s)	2234, 2336, 2414
SmoI	CTYRAG	3 cut(s)	2234, 2336, 2414
SpeI	ACTAGT	1 cut(s)	16
SphI	GCATGC	1 cut(s)	2182
SsiI	CCGC	3 cut(s)	1465, 1536, 1748
SspMI	CTAG	6 cut(s)	17, 210, 1431, 1490, 1565, 2513
StyD4I	CCNGG	4 cut(s)	546, 1308, 2115, 2162
StyI	CCWWGG	3 cut(s)	828, 1342, 1352
TaaI	ACNGT	6 cut(s)	376, 506, 781, 1087, 1697, 2432
TaiI	ACGT	2 cut(s)	362, 1884
TaqI	TCGA	6 cut(s)	122, 253, 542, 723, 1223, 2445
TatI	WGTACW	2 cut(s)	394, 1982
TauI	GCSGC	1 cut(s)	1467
TfiI	GAWTC	4 cut(s)	1070, 1717, 2203, 2314
Tru1I	TTAA	6 cut(s)	522, 641, 684, 1590, 1992, 2235
Tru9I	TTAA	6 cut(s)	522, 641, 684, 1590, 1992, 2235
TseFI	GTSAC	6 cut(s)	130, 379, 775, 781, 1019, 1117
TseI	GCWGC	8 cut(s)	1305, 1357, 1434, 1823, 1856, 2035, 2038, 2486
Tsp45I	GTSAC	6 cut(s)	130, 379, 775, 781, 1019, 1117
TspDTI	ATGAA	9 cut(s)	26, 311, 707, 958, 1431, 1462, 1716, 1743, 2337
Van91I	CCANNNNNTGG	1 cut(s)	2194
Vha464I	CTTAAG	1 cut(s)	2234
VpaK11BI	GGWCC	7 cut(s)	313, 653, 1097, 1745, 1955, 2185, 2197
XagI	CCTNNNNNAGG	1 cut(s)	1341
XapI	RAATTY	6 cut(s)	196, 255, 281, 340, 1628, 1809
XceI	RCATGY	1 cut(s)	2182
XmiI	GTMKAC	1 cut(s)	723
XmnI	GAANNNNTTC	1 cut(s)	407
XspI	CTAG	6 cut(s)	17, 210, 1431, 1490, 1565, 2513
ZraI	GACGTC	1 cut(s)	360

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.