Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007931

Physical Location & Seq

Genomic Coordinates Forward (+)

30840292 .. 30846364

Gene Structure 6073 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1223400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3534 bp

ATGGACTCCAGAACTCGCCTTGACCATGTTCTCTTTCAACTCACTCCAACCAGGACAAGATGTGAACTGGTGATATTTGCTGCTGGTGGTGGTGCAAACGAGAAATTGGCTTCAGGGCTTCTGGAACCATTCCTTGGTCACCTGAAATGCGCCAAGGATCAGATTTCCAAAGGAGGGTACTCCATCATTCTTCGTCCTTCGGACTCCGGTGCCTCCTGGTTCACCAAAGCCACTTTGCAGAGGTTTGTGAAATTTGTTAGCACGCCAGAAGTTCTTGAGAGATTTATGACTATTGAGAGGGAGATTTTGCAAATTGAGAATTCAATTCAATCGAGTGAACTAGCCGAATCAGAAGATGGGAATCAAAATAAGTCAACTGCAATCAAGTCAAACAGTGAATCCAATGGAGCCATTAATGCTGTGCCAGAAGAAAATTCCAAGATTCGTCTTCAACGTGTTTTGGAAACCCGGAAGGTAGTACTATGCAAAGAGCAAGCGATGGCTTATGCTCGTGCTTTAGTTGCTGGATTTGAACTGGACTACATAGAAGATCTTATATCTTTTGCTGATACTTTTGGAGCTACACGCTTAAGGGAAGCATGCATTAATTTCATAAATCTATACAAGCAAAAGAACGAAGATAGGCTTTGGATGGAAGAAATAGCAGCAATGCAGGCACTTTCTCAGCCACAACTGCCGTACTTGGGAACATCAGGAATAATTCTTGCTGGAGAAGATAATGACCCCCATCAGAATCTCATGATAAATGTTAACCAAAACATTCTTTCTGTTGGGAAGAATAGCTCATTAGACACATTAGTGTCGGAGTCAACAAGTCATGGAAGTCTCGACGCGAACCAAGATAATAGCTTGCCAACATCGATGTCATCAATGGATGGGAAGGCACAAGTACCAAACCCATGGCCAAACCAGCATCCTCAGTACATGCACAATTTCCAAGGTCCTGTGTATCCACAAAGGCACCCGTATCAAGGTTACCTTTTTCCTGGTATGCAGGTTCCGACATATTATCCAGGGAATATGAACTGGCCTCCGAATGGGGAAGAATCTGGCCCTATTTTTGATCAGGAATCCGATGGACGGAGGAATCGTAAATCTCATAGGAATAAAAAGAAACATTCTCATGAAAAAGTAGTAGAAACTTCAGACCAAGATGTATCCGGTGACAACACTGGCTCCAGTTATGAGAGTGAATCTGATGATCAGACGCATAAGCAAAGGCATGGGAGAAAGTCCTCGAGAAAGGTTGTTATCCGAAATATCAATTACATAACCTCTAAGAGGGATGGAGAAACAGGAAGTACGTCTGAGGGAAATTCATCCGACAAGGATGGATTTGTTGATGGGAAATCCATTAAACAGCAGGTAGAGGAAGCTGTTGGGTCATTTGAGAAGAAACATAAATCAACCTCACATCGTCATAAAAAACAAGGTGGAGGCAAGTTCCGTGGCGCTGTGGATGATTCAAATAGTGGTGTTGCAAGTACTTATGAGGGAGAAAAACAAAATGAAAACTGGAATGCTTTCCAAGACCTTCTCATGAGGGACAAGGATGAAAGTTCGTTTGCTACAGAACCACACAATGTACAGATTGAGGAGGAATATTTTTCAAGTAAGAACTCTGGCGAAGGAAAAGTGACTAAGCAACGAGCAGATTCAAGTGAATTTTTTGTGGTGACAGAGAGGGATTCAAGTAATGATAGTAAACCACGTGTCCAATACTTCGAAGGTGACGAGAATGTTGGCCGGATTACCAAGAAAGAAGATAGCACATATGAGGACGTATTATTTTCACGGAGAACTGAAGAATCTGGAAACAAATCCCATGATACTTTATCTGATTGTGTCAATGAATCGTACATAACCAAATGTTCTAAAGAAGGGGATTGGTTCATGAACAACCAAACAGATATATCAGCAAATAGGGATGTCAATAATGATCTGAAATTGTTTGATGGTGTTGATGCAATTCATGCGGAGAGGAATAAGAGAGATGTTCTAGGGGATGACTCTTTCATGGTTCAAGGCCGATCACTAGTTGATCAATCTGATTCTCAGTTCAGGACAGATATAAGCTTCGTCCCAGAGATCAATGGAGCTACTCAAGATGAATATGGCATGCAAGAAACTTCAAATGATAAACCTGAAGCGTATAGTGTTCATGAACCGGATGACCTTTACATGATGCTTGACCGTGGATCAGCTATGGAGCATGCTGTGGCACCATGGACTCCCGAAATGGATTATGAAACTATTGCTTCATCATTTGAAGCTACTAAGAAGAATCCTGGCACTGAAGCAAGTGATTCCGTTGAAGTTAAGCAGCCTTCTGATGGTAAGGGAAGGAATGATAAAAATAGTGGAATTCCTGGACAAAAAGCCAGGTCTAAAGTTGTAAATGGATCTTTGGCGAAAAGCAAGTCTGATGTCATGTCAAGAAGCAAGAAACCTGCTCCTGTAAGCAAAAGCACAGTCCACAAGAGCAAATCTGAAATGGAAAAAGAGCGGAGAAAGAGAATGGAGGAGTTACTGATTCAACGCCAGAAGAGAATTGCTGAAAGGAGTGGTTCTGATACAGCAATATCTAAGAAGGCCCCAATGGACAATAAAACTACCAAAATCTCCATGACTAACTCGAAGAATGAGACTAAGAAATCAGATAAGCCTGTCATGCGGAATTCCACCATTGAACGCCTTGCAACTGCACGAGTAACTGAAAAGTTAACACCAACTTTGCCAAGTGCTGGCCAACCCAAAAAACAGAACATCAAGGCAAACGGTGTGGTTGCAAGTGCCTCATCACAGAAGGCAGCTGCTGCAGTGAATAAGAAACCCACTCCAAACAAAGCCAAACCTTTGGGTACAGAAGAGGACCTGAAGAAATCAAATCAATTAACTTCATCTGACTCTAATGTCCAGGAAAAGGTTTGCATAGAATCCACAGAAGCACTGCCGGTTAAGTCAGCAGCTGCAGTTGTAACTCAACCCACCTGTTCCATCAATCATTTGGAAGAGAAAAAAGAAATCCATGGTACATCATCTGTTGAAAAGAATGAGGGAAATTTGATGTTTCAAAGAGAGGCCCTGGAGAATGGTAGCACCAATGGGTATTCTCCCAATTTGGTTTCATCTGTACCTTTTGAAGAGAACGCTCAAAAACTGAATCAGTTTACAGGAGATGTTGAAGAGTTGCCTAAAGAATTTCCAGTTCTGAGTGAAGACAAGAGAAATTATATCTCGGAAATGAGTGTATCTCCTCCTATTCTGGGATCACCTAAGAAAGCTTTGATTGTTTCAGCTGTGAACAATGAAGAAACTGGTGCAAAAACAAAGAATTTACCAATTTCTGAGATCTCTGAGATAGAAATCTCTACTCCACCAAGTGATGAAACACTTGCGGAACTCCACTCCAGGAAGAAGTGGAACAGTGATGAGAACTCTCCTAAAGCTGCCAAAGGGTTTAAGAAGCTTCTATTGTTTGGGCGGAAGAGCCGAAACACTCCTGTCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006355 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009718 GO:0009812 GO:0009813 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015994 GO:0015995 GO:0016020 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033013 GO:0033014 GO:0034641 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046148 GO:0046283 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051186 GO:0051188 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0071944 GO:0080090 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1178

Amino Acids

130.69

Weight (kDa)

6.06

Isoelectric Point (pI)

47.34

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007931)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27430 AT4G27430
fragaria_vesca	FvH4_3g20630 FvH4_3g20630 FvH4_3g20630 FvH4_3g20630 FvH4_3g20630
malus_domestica	MD03G1223400.v1.1 MD11G1241400.v1.1
prunus_persica	Prupe.4G186100_v2.0.a1 Prupe.4G186100_v2.0.a1
pyrus_communis	pycom03g17170 pycom11g21290 pycom11g21300
rosa_chinensis	RchiOBHm_Chr5g0034651
rosa_laevigata	RLG00000033565
rosa_multiflora	Rmu_sc0002460.1_g000082
rosa_roxburghii	Rroxscaffold_1G00045660
rosa_rugosa	Rorug05G0147400
rosa_samantha	Rh5AG239600 Rh5BG240300 Rh5CG270700 Rh5DG248500
rosa_wichuraiana	Rw5G021950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2933
Acc36I	ACCTGC	3 cut(s)	1006, 1375, 2479
AccB1I	GGYRCC	3 cut(s)	209, 981, 2242
AccB7I	CCANNNNNTGG	2 cut(s)	134, 2765
AccBSI	CCGCTC	1 cut(s)	2527
AccII	CGCG	1 cut(s)	854
AciI	CCGC	5 cut(s)	1997, 2527, 2695, 3419, 3505
AclWI	GGATC	4 cut(s)	165, 2227, 2431, 3298
AcoI	YGGCCR	3 cut(s)	923, 1765, 2767
AcuI	CTGAAG	6 cut(s)	96, 1149, 1845, 2187, 2337, 2918
AcvI	CACGTG	1 cut(s)	1733
AdeI	CACNNNGTG	1 cut(s)	3404
AflII	CTTAAG	1 cut(s)	589
AflIII	ACRYGT	2 cut(s)	454, 1732
AhlI	ACTAGT	1 cut(s)	2056
AjuI	GAANNNNNNNTTGG	2 cut(s)	117, 149
AleI	CACNNNNGTG	1 cut(s)	818
Alw26I	GTCTC	2 cut(s)	851, 2660
AlwI	GGATC	4 cut(s)	165, 2227, 2431, 3298
AlwNI	CAGNNNCTG	2 cut(s)	2837, 2990
Ama87I	CYCGRG	1 cut(s)	1258
AoxI	GGCC	8 cut(s)	923, 1049, 1072, 1765, 2047, 2613, 2767, 3102
ApeKI	GCWGC	9 cut(s)	80, 665, 2344, 2831, 2834, 2837, 2987, 2990, 3470
ArsI	GACNNNNNNTTYG	4 cut(s)	2426, 2458, 2478, 2510
AseI	ATTAAT	2 cut(s)	414, 606
Asp700I	GAANNNNTTC	1 cut(s)	1544
AspLEI	GCGC	2 cut(s)	152, 1475
AspS9I	GGNCC	5 cut(s)	962, 1073, 2614, 2893, 3103
AsuC2I	CCSGG	1 cut(s)	469
AsuHPI	GGTGA	7 cut(s)	82, 131, 214, 1196, 1708, 1763, 3285
AsuII	TTCGAA	1 cut(s)	1746
AvaI	CYCGRG	1 cut(s)	1258
AvaII	GGWCC	2 cut(s)	962, 2893
BalI	TGGCCA	2 cut(s)	925, 2769
BanI	GGYRCC	3 cut(s)	209, 981, 2242
BauI	CACGAG	2 cut(s)	510, 2727
BbrPI	CACGTG	1 cut(s)	1733
BbsI	GAAGAC	2 cut(s)	440, 3246
BbvI	GCAGC	9 cut(s)	67, 677, 2356, 2821, 2824, 2843, 2977, 2999, 3457
BceAI	ACGGC	1 cut(s)	682
BciVI	GTATCC	2 cut(s)	981, 1189
BclI	TGATCA	3 cut(s)	1084, 1222, 2062
BcnI	CCSGG	1 cut(s)	469
BcoDI	GTCTC	2 cut(s)	851, 2660
BcuI	ACTAGT	1 cut(s)	2056
BfaI	CTAG	3 cut(s)	341, 2021, 2057
BfmI	CTRYAG	3 cut(s)	1590, 2838, 2991
BfoI	RGCGCY	1 cut(s)	1476
BfrI	CTTAAG	1 cut(s)	589
BfuAI	ACCTGC	3 cut(s)	1006, 1375, 2479
BfuI	GTATCC	2 cut(s)	981, 1189
BglII	AGATCT	2 cut(s)	550, 3372
BisI	GCNGC	9 cut(s)	81, 666, 2345, 2832, 2835, 2838, 2988, 2991, 3471
BlsI	GCNGC	9 cut(s)	82, 667, 2346, 2833, 2836, 2839, 2989, 2992, 3472
BmcAI	AGTACT	2 cut(s)	480, 1507
Bme18I	GGWCC	2 cut(s)	962, 2893
BmeT110I	CYCGRG	1 cut(s)	1258
BmgT120I	GGNCC	5 cut(s)	962, 1073, 2614, 2893, 3103
BmiI	GGNNCC	8 cut(s)	126, 211, 409, 983, 1020, 1198, 2244, 2616
BmsI	GCATC	3 cut(s)	943, 1975, 2196
BpiI	GAAGAC	2 cut(s)	440, 3246
BplI	GAGNNNNNCTC	2 cut(s)	3259, 3291
BpmI	CTGGAG	4 cut(s)	750, 1183, 3128, 3415
Bpu14I	TTCGAA	1 cut(s)	1746
BpuEI	CTTGAG	2 cut(s)	296, 2109
BpuMI	CCSGG	1 cut(s)	469
Bsa29I	ATCGAT	1 cut(s)	881
BsaAI	YACGTR	1 cut(s)	1733
BsaBI	GATNNNNATC	2 cut(s)	360, 3386
BsaWI	WCCGGW	3 cut(s)	206, 1181, 2188
BsaXI	ACNNNNNCTCC	4 cut(s)	1096, 1126, 1181, 1211
Bse118I	RCCGGY	1 cut(s)	2974
Bse1I	ACTGG	8 cut(s)	72, 540, 1052, 1198, 1200, 1541, 3227, 3343
Bse3DI	GCAATG	1 cut(s)	675
Bse8I	GATNNNNATC	2 cut(s)	360, 3386
BseCI	ATCGAT	1 cut(s)	881
BseJI	GATNNNNATC	2 cut(s)	360, 3386
BseMI	GCAATG	1 cut(s)	675
BseMII	CTCAG	7 cut(s)	698, 953, 1320, 2090, 3224, 3360, 3369
BseNI	ACTGG	8 cut(s)	72, 540, 1052, 1198, 1200, 1541, 3227, 3343
BseRI	GAGGAG	3 cut(s)	1631, 2558, 3267
BseXI	GCAGC	9 cut(s)	67, 677, 2356, 2821, 2824, 2843, 2977, 2999, 3457
BsgI	GTGCAG	1 cut(s)	2709
Bsh1236I	CGCG	1 cut(s)	854
BshFI	GGCC	8 cut(s)	925, 1051, 1074, 1767, 2049, 2615, 2769, 3104
BshNI	GGYRCC	3 cut(s)	209, 981, 2242
BshVI	ATCGAT	1 cut(s)	881
BsiHKCI	CYCGRG	1 cut(s)	1258
BsiSI	CCGG	6 cut(s)	207, 469, 1182, 1768, 2189, 2975
BslFI	GGGAC	2 cut(s)	1580, 2087
BsmAI	GTCTC	2 cut(s)	851, 2660
BsmFI	GGGAC	2 cut(s)	1580, 2087
BsmI	GAATGC	1 cut(s)	1546
BsnI	GGCC	8 cut(s)	925, 1051, 1074, 1767, 2049, 2615, 2769, 3104
BsoBI	CYCGRG	1 cut(s)	1258
Bsp119I	TTCGAA	1 cut(s)	1746
Bsp1407I	TGTACA	1 cut(s)	1606
Bsp19I	CCATGG	3 cut(s)	920, 2246, 3049
BspACI	CCGC	5 cut(s)	1997, 2527, 2695, 3419, 3505
BspANI	GGCC	8 cut(s)	925, 1051, 1074, 1767, 2049, 2615, 2769, 3104
BspCNI	CTCAG	7 cut(s)	697, 952, 1321, 2089, 3225, 3361, 3370
BspDI	ATCGAT	1 cut(s)	881
BspFNI	CGCG	1 cut(s)	854
BspHI	TCATGA	5 cut(s)	759, 1144, 1560, 1914, 2182
BspLI	GGNNCC	8 cut(s)	126, 211, 409, 983, 1020, 1198, 2244, 2616
BspMAI	CTGCAG	2 cut(s)	2842, 2995
BspMI	ACCTGC	3 cut(s)	1006, 1375, 2479
BspPI	GGATC	4 cut(s)	165, 2227, 2431, 3298
BspQI	GCTCTTC	1 cut(s)	3503
BspT104I	TTCGAA	1 cut(s)	1746
BspT107I	GGYRCC	3 cut(s)	209, 981, 2242
BspTI	CTTAAG	1 cut(s)	589
BsrBI	CCGCTC	1 cut(s)	2527
BsrDI	GCAATG	1 cut(s)	675
BsrFI	RCCGGY	1 cut(s)	2974
BsrGI	TGTACA	1 cut(s)	1606
BsrI	ACTGG	8 cut(s)	72, 540, 1052, 1198, 1200, 1541, 3227, 3343
BssAI	RCCGGY	1 cut(s)	2974
BssSI	CACGAG	2 cut(s)	510, 2727
BssT1I	CCWWGG	6 cut(s)	133, 153, 920, 958, 2246, 3049
Bst2BI	CACGAG	2 cut(s)	510, 2727
Bst4CI	ACNGT	5 cut(s)	395, 2216, 2494, 2801, 3449
Bst6I	CTCTTC	6 cut(s)	2561, 2883, 3027, 3159, 3201, 3503
BstAFI	CTTAAG	1 cut(s)	589
BstAPI	GCANNNNNTGC	2 cut(s)	1994, 2837
BstAUI	TGTACA	1 cut(s)	1606
BstBAI	YACGTR	1 cut(s)	1733
BstBI	TTCGAA	1 cut(s)	1746
BstC8I	GCNNGC	8 cut(s)	263, 495, 601, 675, 872, 2141, 2235, 2767
BstDSI	CCRYGG	5 cut(s)	920, 1468, 2215, 2246, 3049
BstEII	GGTNACC	2 cut(s)	137, 995
BstENI	CCTNNNNNAGG	1 cut(s)	1300
BstFNI	CGCG	1 cut(s)	854
BstH2I	RGCGCY	1 cut(s)	1476
BstHHI	GCGC	2 cut(s)	152, 1475
BstMAI	GTCTC	2 cut(s)	851, 2660
BstMWI	GCNNNNNNNGC	8 cut(s)	416, 521, 674, 694, 931, 1994, 2692, 2837
BstNSI	RCATGY	4 cut(s)	603, 949, 2143, 2237
BstPI	GGTNACC	2 cut(s)	137, 995
BstSFI	CTRYAG	3 cut(s)	1590, 2838, 2991
BstUI	CGCG	1 cut(s)	854
BstV1I	GCAGC	9 cut(s)	67, 677, 2356, 2821, 2824, 2843, 2977, 2999, 3457
BstV2I	GAAGAC	2 cut(s)	440, 3246
BstX2I	RGATCY	3 cut(s)	550, 2423, 3372
BstXI	CCANNNNNNTGG	2 cut(s)	921, 2878
BstYI	RGATCY	3 cut(s)	550, 2423, 3372
Bsu15I	ATCGAT	1 cut(s)	881
BsuI	GTATCC	2 cut(s)	981, 1189
BsuRI	GGCC	8 cut(s)	925, 1051, 1074, 1767, 2049, 2615, 2769, 3104
BsuTUI	ATCGAT	1 cut(s)	881
BtgI	CCRYGG	5 cut(s)	920, 1468, 2215, 2246, 3049
BtgZI	GCGATG	1 cut(s)	512
BtsI	GCAGTG	2 cut(s)	2847, 2969
BtsIMutI	CAGTG	6 cut(s)	400, 1191, 2313, 2847, 2969, 3454
BveI	ACCTGC	3 cut(s)	1006, 1375, 2479
Cac8I	GCNNGC	8 cut(s)	263, 495, 601, 675, 872, 2141, 2235, 2767
CaiI	CAGNNNCTG	2 cut(s)	2837, 2990
CciI	TCATGA	5 cut(s)	759, 1144, 1560, 1914, 2182
CfoI	GCGC	2 cut(s)	152, 1475
Cfr10I	RCCGGY	1 cut(s)	2974
Cfr13I	GGNCC	5 cut(s)	962, 1073, 2614, 2893, 3103
ClaI	ATCGAT	1 cut(s)	881
CseI	GACGC	2 cut(s)	860, 1237
CspCI	CAANNNNNGTGG	4 cut(s)	1450, 1485, 2784, 2819
DraIII	CACNNNGTG	1 cut(s)	3404
DrdI	GACNNNNNNGTC	1 cut(s)	2933
DseDI	GACNNNNNNGTC	1 cut(s)	2933
EaeI	YGGCCR	3 cut(s)	923, 1765, 2767
Eam1104I	CTCTTC	6 cut(s)	2561, 2883, 3027, 3159, 3201, 3503
EarI	CTCTTC	6 cut(s)	2561, 2883, 3027, 3159, 3201, 3503
EciI	GGCGGA	1 cut(s)	3520
Eco130I	CCWWGG	6 cut(s)	133, 153, 920, 958, 2246, 3049
Eco47I	GGWCC	2 cut(s)	962, 2893
Eco57I	CTGAAG	6 cut(s)	96, 1149, 1845, 2187, 2337, 2918
Eco72I	CACGTG	1 cut(s)	1733
Eco88I	CYCGRG	1 cut(s)	1258
Eco91I	GGTNACC	2 cut(s)	137, 995
EcoNI	CCTNNNNNAGG	1 cut(s)	1300
EcoO109I	RGGNCCY	4 cut(s)	962, 2614, 2893, 3103
EcoO65I	GGTNACC	2 cut(s)	137, 995
EcoRI	GAATTC	3 cut(s)	319, 2385, 2698
EcoT14I	CCWWGG	6 cut(s)	133, 153, 920, 958, 2246, 3049
EcoT22I	ATGCAT	1 cut(s)	605
ErhI	CCWWGG	6 cut(s)	133, 153, 920, 958, 2246, 3049
FalI	AAGNNNNNCTT	4 cut(s)	630, 662, 984, 1016
FaqI	GGGAC	2 cut(s)	1580, 2087
FauNDI	CATATG	1 cut(s)	1795
FbaI	TGATCA	3 cut(s)	1084, 1222, 2062
Fnu4HI	GCNGC	9 cut(s)	81, 666, 2345, 2832, 2835, 2838, 2988, 2991, 3471
Fsp4HI	GCNGC	9 cut(s)	81, 666, 2345, 2832, 2835, 2838, 2988, 2991, 3471
FspBI	CTAG	3 cut(s)	341, 2021, 2057
GlaI	GCGC	2 cut(s)	151, 1474
GluI	GCNGC	9 cut(s)	81, 666, 2345, 2832, 2835, 2838, 2988, 2991, 3471
GsuI	CTGGAG	4 cut(s)	750, 1183, 3128, 3415
HaeII	RGCGCY	1 cut(s)	1476
HaeIII	GGCC	8 cut(s)	925, 1051, 1074, 1767, 2049, 2615, 2769, 3104
HapII	CCGG	6 cut(s)	207, 469, 1182, 1768, 2189, 2975
HgaI	GACGC	2 cut(s)	860, 1237
HhaI	GCGC	2 cut(s)	152, 1475
Hin6I	GCGC	2 cut(s)	150, 1473
HinP1I	GCGC	2 cut(s)	150, 1473
HincII	GTYRAC	4 cut(s)	375, 772, 831, 2745
HindII	GTYRAC	4 cut(s)	375, 772, 831, 2745
HindIII	AAGCTT	3 cut(s)	2095, 3303, 3488
HpaI	GTTAAC	2 cut(s)	772, 2745
HpaII	CCGG	6 cut(s)	207, 469, 1182, 1768, 2189, 2975
HphI	GGTGA	7 cut(s)	82, 131, 214, 1196, 1708, 1763, 3285
Hpy99I	CGWCG	1 cut(s)	854
HpyCH4III	ACNGT	5 cut(s)	395, 2216, 2494, 2801, 3449
HpyCH4IV	ACGT	4 cut(s)	454, 1325, 1732, 1803
HpyF10VI	GCNNNNNNNGC	8 cut(s)	416, 521, 674, 694, 931, 1994, 2692, 2837
HpySE526I	ACGT	4 cut(s)	454, 1325, 1732, 1803
HspAI	GCGC	2 cut(s)	150, 1473
Ksp22I	TGATCA	3 cut(s)	1084, 1222, 2062
KspAI	GTTAAC	2 cut(s)	772, 2745
LguI	GCTCTTC	1 cut(s)	3503
LmnI	GCTCC	6 cut(s)	407, 578, 1202, 2117, 2230, 2479
Lsp1109I	GCAGC	9 cut(s)	67, 677, 2356, 2821, 2824, 2843, 2977, 2999, 3457
LweI	GCATC	3 cut(s)	943, 1975, 2196
MaeI	CTAG	3 cut(s)	341, 2021, 2057
MaeII	ACGT	4 cut(s)	454, 1325, 1732, 1803
MaeIII	GTNAC	9 cut(s)	137, 995, 1184, 1657, 1696, 1751, 2547, 2731, 2998
MbiI	CCGCTC	1 cut(s)	2527
MfeI	CAATTG	1 cut(s)	3529
MflI	RGATCY	3 cut(s)	550, 2423, 3372
MlsI	TGGCCA	2 cut(s)	925, 2769
MluNI	TGGCCA	2 cut(s)	925, 2769
MlyI	GAGTC	5 cut(s)	197, 836, 2024, 2245, 2921
MmeI	TCCRAC	4 cut(s)	71, 804, 1046, 1368
Mox20I	TGGCCA	2 cut(s)	925, 2769
Mph1103I	ATGCAT	1 cut(s)	605
MroXI	GAANNNNTTC	1 cut(s)	1544
MscI	TGGCCA	2 cut(s)	925, 2769
MslI	CAYNNNNRTG	2 cut(s)	818, 1143
Msp20I	TGGCCA	2 cut(s)	925, 2769
MspA1I	CMGCKG	3 cut(s)	2834, 2990, 3320
MspCI	CTTAAG	1 cut(s)	589
MspI	CCGG	6 cut(s)	207, 469, 1182, 1768, 2189, 2975
MunI	CAATTG	1 cut(s)	3529
Mva1269I	GAATGC	1 cut(s)	1546
MvnI	CGCG	1 cut(s)	854
MwoI	GCNNNNNNNGC	8 cut(s)	416, 521, 674, 694, 931, 1994, 2692, 2837
NciI	CCSGG	1 cut(s)	469
NcoI	CCATGG	3 cut(s)	920, 2246, 3049
NdeI	CATATG	1 cut(s)	1795
NlaIV	GGNNCC	8 cut(s)	126, 211, 409, 983, 1020, 1198, 2244, 2616
NmuCI	GTSAC	5 cut(s)	137, 1184, 1657, 1696, 1751
NsiI	ATGCAT	1 cut(s)	605
NspI	RCATGY	4 cut(s)	603, 949, 2143, 2237
NspV	TTCGAA	1 cut(s)	1746
OliI	CACNNNNGTG	1 cut(s)	818
PaeI	GCATGC	3 cut(s)	603, 2143, 2237
PaeR7I	CTCGAG	1 cut(s)	1258
PagI	TCATGA	5 cut(s)	759, 1144, 1560, 1914, 2182
PciSI	GCTCTTC	1 cut(s)	3503
PcsI	WCGNNNNNNNCGW	3 cut(s)	451, 1108, 1752
PctI	GAATGC	1 cut(s)	1546
PdmI	GAANNNNTTC	1 cut(s)	1544
PflMI	CCANNNNNTGG	2 cut(s)	134, 2765
PfoI	TCCNGGA	3 cut(s)	2389, 2937, 3431
PkrI	GCNGC	9 cut(s)	82, 667, 2346, 2833, 2836, 2839, 2989, 2992, 3472
PleI	GAGTC	5 cut(s)	197, 835, 2024, 2245, 2921
PmaCI	CACGTG	1 cut(s)	1733
PmlI	CACGTG	1 cut(s)	1733
PpsI	GAGTC	5 cut(s)	197, 835, 2024, 2245, 2921
Ppu21I	YACGTR	1 cut(s)	1733
PpuMI	RGGWCCY	2 cut(s)	962, 2893
PshBI	ATTAAT	2 cut(s)	414, 606
Psp5II	RGGWCCY	2 cut(s)	962, 2893
PspCI	CACGTG	1 cut(s)	1733
PspEI	GGTNACC	2 cut(s)	137, 995
PspN4I	GGNNCC	8 cut(s)	126, 211, 409, 983, 1020, 1198, 2244, 2616
PspPI	GGNCC	5 cut(s)	962, 1073, 2614, 2893, 3103
PspPPI	RGGWCCY	2 cut(s)	962, 2893
PstI	CTGCAG	2 cut(s)	2842, 2995
PstNI	CAGNNNCTG	2 cut(s)	2837, 2990
PsuI	RGATCY	3 cut(s)	550, 2423, 3372
PvuII	CAGCTG	3 cut(s)	2834, 2990, 3320
RseI	CAYNNNNRTG	2 cut(s)	818, 1143
SapI	GCTCTTC	1 cut(s)	3503
SatI	GCNGC	9 cut(s)	81, 666, 2345, 2832, 2835, 2838, 2988, 2991, 3471
Sau96I	GGNCC	5 cut(s)	962, 1073, 2614, 2893, 3103
ScaI	AGTACT	2 cut(s)	480, 1507
SchI	GAGTC	5 cut(s)	197, 836, 2024, 2245, 2921
SfaNI	GCATC	3 cut(s)	943, 1975, 2196
SfcI	CTRYAG	3 cut(s)	1590, 2838, 2991
Sfr274I	CTCGAG	1 cut(s)	1258
SfuI	TTCGAA	1 cut(s)	1746
SinI	GGWCC	2 cut(s)	962, 2893
SlaI	CTCGAG	1 cut(s)	1258
SmiMI	CAYNNNNRTG	2 cut(s)	818, 1143
SmlI	CTYRAG	4 cut(s)	275, 589, 1258, 2124
SmoI	CTYRAG	4 cut(s)	275, 589, 1258, 2124
SpeI	ACTAGT	1 cut(s)	2056
SphI	GCATGC	3 cut(s)	603, 2143, 2237
SsiI	CCGC	5 cut(s)	1997, 2527, 2695, 3419, 3505
SspI	AATATT	1 cut(s)	1625
SspMI	CTAG	3 cut(s)	341, 2021, 2057
StyI	CCWWGG	6 cut(s)	133, 153, 920, 958, 2246, 3049
TaaI	ACNGT	5 cut(s)	395, 2216, 2494, 2801, 3449
TaiI	ACGT	4 cut(s)	457, 1328, 1735, 1806
TaqI	TCGA	6 cut(s)	332, 849, 881, 1259, 1746, 2657
TatI	WGTACW	4 cut(s)	478, 942, 1505, 1606
TscAI	CASTG	6 cut(s)	400, 1198, 2320, 2847, 2976, 3454
TseFI	GTSAC	5 cut(s)	137, 1184, 1657, 1696, 1751
TseI	GCWGC	9 cut(s)	80, 665, 2344, 2831, 2834, 2837, 2987, 2990, 3470
Tsp45I	GTSAC	5 cut(s)	137, 1184, 1657, 1696, 1751
TspGWI	ACGGA	4 cut(s)	1117, 1457, 1831, 2320
TspRI	CASTG	6 cut(s)	400, 1198, 2320, 2847, 2976, 3454
Van91I	CCANNNNNTGG	2 cut(s)	134, 2765
Vha464I	CTTAAG	1 cut(s)	589
VpaK11BI	GGWCC	2 cut(s)	962, 2893
VspI	ATTAAT	2 cut(s)	414, 606
XagI	CCTNNNNNAGG	1 cut(s)	1300
XceI	RCATGY	4 cut(s)	603, 949, 2143, 2237
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3025
XhoI	CTCGAG	1 cut(s)	1258
XmnI	GAANNNNTTC	1 cut(s)	1544
XspI	CTAG	3 cut(s)	341, 2021, 2057
ZrmI	AGTACT	2 cut(s)	480, 1507
Zsp2I	ATGCAT	1 cut(s)	605

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.