Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0007931

Physical Location & Seq

Genomic Coordinates Forward (+)

18360269 .. 18362747

Gene Structure 2479 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g17170.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2160 bp

ATGGATCCGGTGACAACACTGGCTCCAGTTATGAGAGTGAATCTGATCGATCAGATGCATAAGCAAAGGCATGGGAGAAAGTCCTCGAGAAGGGTGGTTATCCGAAATATCAATTACATAACCTCTAAGAGGGATGGAGAAACAGGAAGTACGTCTGAGGGAAATTCATCCGACGAGGATGGATTTGTTGATGGCAAATCCATTAAACAGCAGGTAGAGGAAGCTGTTGGGTCATTTGAGAAGAAACATAAATCAACCTCACATCGTCATAAAAAACAAGGTGGAGGCAAGTTCCGTGGTGCTGTGGATGATTCAAATAGTGGTGTTGCAAGTACTTATGAGGGAGAAAAACAAAATGAAAACTGGAATGCTTTCCAAGACCTTCTCATGAGGGACAAGGATGAAAGTTCGTTTGCTATAGAACCACACAATGTACAGATTGAGGAGGAATATTTTTCAAGTAAGAACTCTGGTGAAGGAAAAGTGACTAAGCAACGAGCAGATTCAAGTGAATTTTTTGTGGTGACAGAGAGGGATTCAATTAATGAGAGTAAACCACGTGTCCAATACTTCGAAGGTGATAAGAATGTTGGCCGGATTACCAAGAAAGAAGATAGCACATATGAGGATGTATTATTTTCACGGAGAACTGAAGAATCCAGGAACAAATCCCGTGATACTTTATCTGATTGTGTCAATGAATCGTACATAACCAAATGTTCTAAAGAAGGGGATTGGTTCATGAACAAACAAACAGATATATCAGCAAATAGGGATGTCAATAATGATCAGAAATTGTTTGATGGTGTTGATGCAATTCATGCGGAGAGAAATAAGAGAGATGTTCTAGGGGATGACTCTTTCATGGTTCAAGACCGATCACTAGTTGATCAATCTGATTCTCAGTTCAGGACAGATATAAGCTTCGTCCCAGAGATCAATGGAGCTACTCAAGATGAATACGGCATGCAAGAAACTTCAAATGATAAACCTGAAGCGTATAGTGATCATGAACCAGATGACCTTTACATGATGCTTGACCGTGGATCAGCTATGGAGCATGCTGTGGCACCATGGACTCCTGAAATGGATTATGAAACTATTGCTTCATCATTTGAAGCTACTAAGAAGAATCCTGGCACTGAAGCAAGTGATTCCGATGAAGTTAAGCAGCCTTCTGATAGTAAGGGAAGGAATGATAAAAATAGTGGAATTCCTGGACAAAAAGCCAGGTCTAAAGTTGTAAATGGATCTTTGGCGAAAAGCAAGTCTGATGTCATGTCAAGAAGCAAGAAACCTGCTCCTGTAAGCAAAAGCACAGTCCACAAGAGCAAATCTGAAATGGAAAAAGAGCGGAGAAAGAGAATGGAGGAGTTACTGATTCAACGCCAGAAGAGAATTGCTGAAAGGAGTGGTTCTAATACAGCAATATCTAAGAAGGCCCCAATGGACAATAAAACTACCAAAATCTCCATGACTAACTCGAAGAATGAGACTAAGAAATCAGATAAGCCTGTCATGCGGAATTCCACCATTGAACGCCTTGCAACCACACGAGCAACTGAAAAGTTAACTCCAACTTTGCCAAGTGCTGGCCAACCCAAAACACAGAACATCAAGGCAAATGGTGTGGTTGCAAGTGCCTCATCACAGAAGGTAGCTGCTGCAGTGAATAAGAAACCCACTCCAAACAAAGCCAAACCCTTGGGTGCAAAAGAGGACCTGAAGAAATCAAATCAATTAATTTCATCTGACTCTAATGTCCAGGAAAAGGTTTGCATAGAATCCACAGAAGCACTGCCGGTTAAGTCAGCAGCTGCGGTTGTAACTCAATCCACCCGTTCCATCAATCATTTGGAAGAGAAAAAAGAAATCCATGGTACATCTTCAGTTGAAAAGAATGAGGGAAATTTGATGTTACAAAGAGAGGCCTTGGAGAACGGTAGCTGCAACGGGTATTCTCCCAATTTGGTTTCATCTGTACCTTTTGAAGAGAAGGCTCAAAAACTGAATCAGTTTACAGGTGATGTTGAAGAGTTGCCTAAAGAATTTACAGTTCTAAGTGAAGACAAGAGAAATTATATCCCGGAAATGAGTGTATCTCCTCCTATTCTGGGATCGACCTACAAAAGCTTTGATTGTTTCAGCTGTGAACAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006355 GO:0006725 GO:0006778 GO:0006779 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009718 GO:0009812 GO:0009813 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0015994 GO:0015995 GO:0016020 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0033013 GO:0033014 GO:0034641 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046148 GO:0046283 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051186 GO:0051188 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071704 GO:0071944 GO:0080090 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

720

Amino Acids

80.18

Weight (kDa)

6.54

Isoelectric Point (pI)

50.55

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007931)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27430 AT4G27430
fragaria_vesca	FvH4_3g20630 FvH4_3g20630 FvH4_3g20630 FvH4_3g20630 FvH4_3g20630
malus_domestica	MD03G1223400.v1.1 MD11G1241400.v1.1
prunus_persica	Prupe.4G186100_v2.0.a1 Prupe.4G186100_v2.0.a1
pyrus_communis	pycom03g17170 pycom11g21290 pycom11g21300
rosa_chinensis	RchiOBHm_Chr5g0034651
rosa_laevigata	RLG00000033565
rosa_multiflora	Rmu_sc0002460.1_g000082
rosa_roxburghii	Rroxscaffold_1G00045660
rosa_rugosa	Rorug05G0147400
rosa_samantha	Rh5AG239600 Rh5BG240300 Rh5CG270700 Rh5DG248500
rosa_wichuraiana	Rw5G021950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1760
Acc36I	ACCTGC	2 cut(s)	202, 1306
AccB1I	GGYRCC	1 cut(s)	1069
AccB7I	CCANNNNNTGG	1 cut(s)	1592
AccBSI	CCGCTC	1 cut(s)	1354
AciI	CCGC	4 cut(s)	824, 1354, 1522, 1820
AclWI	GGATC	4 cut(s)	12, 1054, 1258, 2125
AcoI	YGGCCR	2 cut(s)	592, 1594
AcsI	RAATTY	6 cut(s)	163, 512, 1212, 1525, 1909, 2048
AcuI	CTGAAG	5 cut(s)	672, 1014, 1164, 1745, 1872
AcvI	CACGTG	1 cut(s)	560
AfaI	GTAC	6 cut(s)	151, 334, 435, 707, 1882, 1983
AfiI	CCNNNNNNNGG	5 cut(s)	90, 129, 1592, 1771, 2114
AflIII	ACRYGT	1 cut(s)	559
AhlI	ACTAGT	1 cut(s)	883
AjnI	CCWGG	5 cut(s)	659, 1135, 1216, 1229, 1764
Alw26I	GTCTC	1 cut(s)	1487
AlwI	GGATC	4 cut(s)	12, 1054, 1258, 2125
AlwNI	CAGNNNCTG	1 cut(s)	1817
Ama87I	CYCGRG	1 cut(s)	85
AoxI	GGCC	4 cut(s)	592, 1440, 1594, 1929
ApeKI	GCWGC	6 cut(s)	1171, 1661, 1664, 1814, 1817, 1947
ApoI	RAATTY	6 cut(s)	163, 512, 1212, 1525, 1909, 2048
ArsI	GACNNNNNNTTYG	4 cut(s)	1253, 1285, 1305, 1337
AseI	ATTAAT	2 cut(s)	543, 1742
Asp700I	GAANNNNTTC	1 cut(s)	371
AspS9I	GGNCC	2 cut(s)	1441, 1720
AsuC2I	CCSGG	1 cut(s)	2087
AsuHPI	GGTGA	5 cut(s)	22, 485, 535, 590, 2036
AsuII	TTCGAA	1 cut(s)	573
AvaI	CYCGRG	1 cut(s)	85
AvaII	GGWCC	1 cut(s)	1720
BalI	TGGCCA	1 cut(s)	1596
BamHI	GGATCC	1 cut(s)	4
BanI	GGYRCC	1 cut(s)	1069
BauI	CACGAG	1 cut(s)	1554
BbrPI	CACGTG	1 cut(s)	560
BbsI	GAAGAC	1 cut(s)	2073
BbvI	GCAGC	6 cut(s)	1183, 1648, 1651, 1804, 1826, 1934
BccI	CCATC	5 cut(s)	128, 173, 185, 797, 1853
BceAI	ACGGC	1 cut(s)	979
BciT130I	CCWGG	5 cut(s)	661, 1137, 1218, 1231, 1766
BclI	TGATCA	3 cut(s)	787, 889, 1006
BcnI	CCSGG	1 cut(s)	2087
BcoDI	GTCTC	1 cut(s)	1487
BcuI	ACTAGT	1 cut(s)	883
BfaI	CTAG	2 cut(s)	848, 884
BfmI	CTRYAG	2 cut(s)	417, 1665
BfuAI	ACCTGC	2 cut(s)	202, 1306
BisI	GCNGC	6 cut(s)	1172, 1662, 1665, 1815, 1818, 1948
BlsI	GCNGC	6 cut(s)	1173, 1663, 1666, 1816, 1819, 1949
BmcAI	AGTACT	1 cut(s)	334
Bme1390I	CCNGG	6 cut(s)	661, 1137, 1218, 1231, 1766, 2087
Bme18I	GGWCC	1 cut(s)	1720
BmeT110I	CYCGRG	1 cut(s)	85
BmgT120I	GGNCC	2 cut(s)	1441, 1720
BmiI	GGNNCC	4 cut(s)	6, 24, 1071, 1443
BmrFI	CCNGG	6 cut(s)	661, 1137, 1218, 1231, 1766, 2087
BmsI	GCATC	3 cut(s)	45, 802, 1023
BpiI	GAAGAC	1 cut(s)	2073
BplI	GAGNNNNNCTC	2 cut(s)	2086, 2118
BpmI	CTGGAG	1 cut(s)	9
Bpu14I	TTCGAA	1 cut(s)	573
BpuEI	CTTGAG	1 cut(s)	936
BpuMI	CCSGG	1 cut(s)	2087
Bsa29I	ATCGAT	1 cut(s)	48
BsaAI	YACGTR	1 cut(s)	560
BsaJI	CCNNGG	6 cut(s)	295, 1042, 1073, 1704, 1876, 1932
BsaWI	WCCGGW	1 cut(s)	7
BsaXI	ACNNNNNCTCC	2 cut(s)	7, 37
Bsc4I	CCNNNNNNNGG	5 cut(s)	90, 129, 1592, 1771, 2114
Bse118I	RCCGGY	1 cut(s)	1801
Bse1I	ACTGG	3 cut(s)	24, 26, 368
BseBI	CCWGG	5 cut(s)	661, 1137, 1218, 1231, 1766
BseCI	ATCGAT	1 cut(s)	48
BseDI	CCNNGG	6 cut(s)	295, 1042, 1073, 1704, 1876, 1932
BseGI	GGATG	8 cut(s)	139, 167, 184, 313, 406, 634, 781, 859
BseLI	CCNNNNNNNGG	5 cut(s)	90, 129, 1592, 1771, 2114
BseMII	CTCAG	2 cut(s)	147, 917
BseNI	ACTGG	3 cut(s)	24, 26, 368
BseRI	GAGGAG	3 cut(s)	458, 1385, 2094
BseXI	GCAGC	6 cut(s)	1183, 1648, 1651, 1804, 1826, 1934
BshFI	GGCC	4 cut(s)	594, 1442, 1596, 1931
BshNI	GGYRCC	1 cut(s)	1069
BshVI	ATCGAT	1 cut(s)	48
BsiHKCI	CYCGRG	1 cut(s)	85
BsiSI	CCGG	4 cut(s)	8, 595, 1802, 2087
BslFI	GGGAC	2 cut(s)	407, 914
BslI	CCNNNNNNNGG	5 cut(s)	90, 129, 1592, 1771, 2114
BsmAI	GTCTC	1 cut(s)	1487
BsmFI	GGGAC	2 cut(s)	407, 914
BsmI	GAATGC	1 cut(s)	373
BsnI	GGCC	4 cut(s)	594, 1442, 1596, 1931
BsoBI	CYCGRG	1 cut(s)	85
Bsp119I	TTCGAA	1 cut(s)	573
Bsp1407I	TGTACA	1 cut(s)	433
Bsp19I	CCATGG	2 cut(s)	1073, 1876
BspACI	CCGC	4 cut(s)	824, 1354, 1522, 1820
BspANI	GGCC	4 cut(s)	594, 1442, 1596, 1931
BspCNI	CTCAG	2 cut(s)	148, 916
BspDI	ATCGAT	1 cut(s)	48
BspHI	TCATGA	3 cut(s)	387, 741, 1009
BspLI	GGNNCC	4 cut(s)	6, 24, 1071, 1443
BspMAI	CTGCAG	1 cut(s)	1669
BspMI	ACCTGC	2 cut(s)	202, 1306
BspPI	GGATC	4 cut(s)	12, 1054, 1258, 2125
BspT104I	TTCGAA	1 cut(s)	573
BspT107I	GGYRCC	1 cut(s)	1069
BsrBI	CCGCTC	1 cut(s)	1354
BsrFI	RCCGGY	1 cut(s)	1801
BsrGI	TGTACA	1 cut(s)	433
BsrI	ACTGG	3 cut(s)	24, 26, 368
BssAI	RCCGGY	1 cut(s)	1801
BssECI	CCNNGG	6 cut(s)	295, 1042, 1073, 1704, 1876, 1932
BssSI	CACGAG	1 cut(s)	1554
BssT1I	CCWWGG	4 cut(s)	1073, 1704, 1876, 1932
Bst2BI	CACGAG	1 cut(s)	1554
Bst2UI	CCWGG	5 cut(s)	661, 1137, 1218, 1231, 1766
Bst4CI	ACNGT	4 cut(s)	1043, 1321, 1943, 2056
Bst6I	CTCTTC	4 cut(s)	1388, 1854, 1986, 2028
BstAPI	GCANNNNNTGC	1 cut(s)	821
BstAUI	TGTACA	1 cut(s)	433
BstBAI	YACGTR	1 cut(s)	560
BstBI	TTCGAA	1 cut(s)	573
BstC8I	GCNNGC	3 cut(s)	968, 1062, 1594
BstDEI	CTNAG	8 cut(s)	126, 156, 489, 903, 1125, 1434, 1497, 2060
BstDSI	CCRYGG	4 cut(s)	295, 1042, 1073, 1876
BstENI	CCTNNNNNAGG	2 cut(s)	88, 127
BstF5I	GGATG	8 cut(s)	139, 167, 184, 313, 406, 634, 781, 859
BstMAI	GTCTC	1 cut(s)	1487
BstMWI	GCNNNNNNNGC	2 cut(s)	821, 1519
BstNI	CCWGG	5 cut(s)	661, 1137, 1218, 1231, 1766
BstNSI	RCATGY	2 cut(s)	970, 1064
BstSCI	CCNGG	6 cut(s)	659, 1135, 1216, 1229, 1764, 2085
BstSFI	CTRYAG	2 cut(s)	417, 1665
BstV1I	GCAGC	6 cut(s)	1183, 1648, 1651, 1804, 1826, 1934
BstV2I	GAAGAC	1 cut(s)	2073
BstX2I	RGATCY	2 cut(s)	4, 1250
BstXI	CCANNNNNNTGG	1 cut(s)	1705
BstYI	RGATCY	2 cut(s)	4, 1250
Bsu15I	ATCGAT	1 cut(s)	48
BsuRI	GGCC	4 cut(s)	594, 1442, 1596, 1931
BsuTUI	ATCGAT	1 cut(s)	48
BtgI	CCRYGG	4 cut(s)	295, 1042, 1073, 1876
BtsCI	GGATG	8 cut(s)	139, 167, 184, 313, 406, 634, 781, 859
BtsI	GCAGTG	2 cut(s)	1674, 1796
BtsIMutI	CAGTG	4 cut(s)	17, 1140, 1674, 1796
BveI	ACCTGC	2 cut(s)	202, 1306
Cac8I	GCNNGC	3 cut(s)	968, 1062, 1594
CaiI	CAGNNNCTG	1 cut(s)	1817
CciI	TCATGA	3 cut(s)	387, 741, 1009
Cfr10I	RCCGGY	1 cut(s)	1801
Cfr13I	GGNCC	2 cut(s)	1441, 1720
ClaI	ATCGAT	1 cut(s)	48
Csp6I	GTAC	6 cut(s)	150, 333, 434, 706, 1881, 1982
CspCI	CAANNNNNGTGG	4 cut(s)	277, 312, 1611, 1646
CviQI	GTAC	6 cut(s)	150, 333, 434, 706, 1881, 1982
DdeI	CTNAG	8 cut(s)	126, 156, 489, 903, 1125, 1434, 1497, 2060
DrdI	GACNNNNNNGTC	1 cut(s)	1760
DseDI	GACNNNNNNGTC	1 cut(s)	1760
EaeI	YGGCCR	2 cut(s)	592, 1594
Eam1104I	CTCTTC	4 cut(s)	1388, 1854, 1986, 2028
EarI	CTCTTC	4 cut(s)	1388, 1854, 1986, 2028
Eco130I	CCWWGG	4 cut(s)	1073, 1704, 1876, 1932
Eco147I	AGGCCT	1 cut(s)	1931
Eco47I	GGWCC	1 cut(s)	1720
Eco57I	CTGAAG	5 cut(s)	672, 1014, 1164, 1745, 1872
Eco72I	CACGTG	1 cut(s)	560
Eco88I	CYCGRG	1 cut(s)	85
EcoNI	CCTNNNNNAGG	2 cut(s)	88, 127
EcoO109I	RGGNCCY	2 cut(s)	1441, 1720
EcoRI	GAATTC	2 cut(s)	1212, 1525
EcoRII	CCWGG	5 cut(s)	659, 1135, 1216, 1229, 1764
EcoT14I	CCWWGG	4 cut(s)	1073, 1704, 1876, 1932
EcoT22I	ATGCAT	1 cut(s)	60
ErhI	CCWWGG	4 cut(s)	1073, 1704, 1876, 1932
FaqI	GGGAC	2 cut(s)	407, 914
FauNDI	CATATG	1 cut(s)	622
FbaI	TGATCA	3 cut(s)	787, 889, 1006
Fnu4HI	GCNGC	6 cut(s)	1172, 1662, 1665, 1815, 1818, 1948
FokI	GGATG	8 cut(s)	146, 154, 191, 320, 413, 641, 788, 866
Fsp4HI	GCNGC	6 cut(s)	1172, 1662, 1665, 1815, 1818, 1948
FspBI	CTAG	2 cut(s)	848, 884
GluI	GCNGC	6 cut(s)	1172, 1662, 1665, 1815, 1818, 1948
GsuI	CTGGAG	1 cut(s)	9
HaeIII	GGCC	4 cut(s)	594, 1442, 1596, 1931
HapII	CCGG	4 cut(s)	8, 595, 1802, 2087
HincII	GTYRAC	1 cut(s)	1572
HindII	GTYRAC	1 cut(s)	1572
HindIII	AAGCTT	2 cut(s)	922, 2131
HpaI	GTTAAC	1 cut(s)	1572
HpaII	CCGG	4 cut(s)	8, 595, 1802, 2087
HphI	GGTGA	5 cut(s)	22, 485, 535, 590, 2036
Hpy166II	GTNNAC	5 cut(s)	554, 1324, 1572, 2019, 2153
Hpy188III	TCNNGA	9 cut(s)	87, 388, 742, 872, 910, 953, 1010, 1082, 1284
Hpy8I	GTNNAC	5 cut(s)	554, 1324, 1572, 2019, 2153
Hpy99I	CGWCG	1 cut(s)	176
HpyCH4III	ACNGT	4 cut(s)	1043, 1321, 1943, 2056
HpyCH4IV	ACGT	2 cut(s)	152, 559
HpyF10VI	GCNNNNNNNGC	2 cut(s)	821, 1519
HpyF3I	CTNAG	8 cut(s)	126, 156, 489, 903, 1125, 1434, 1497, 2060
HpySE526I	ACGT	2 cut(s)	152, 559
Ksp22I	TGATCA	3 cut(s)	787, 889, 1006
KspAI	GTTAAC	1 cut(s)	1572
LmnI	GCTCC	4 cut(s)	28, 944, 1057, 1306
Lsp1109I	GCAGC	6 cut(s)	1183, 1648, 1651, 1804, 1826, 1934
LweI	GCATC	3 cut(s)	45, 802, 1023
MaeI	CTAG	2 cut(s)	848, 884
MaeII	ACGT	2 cut(s)	152, 559
MaeIII	GTNAC	6 cut(s)	10, 484, 523, 1374, 1825, 1917
MbiI	CCGCTC	1 cut(s)	1354
MflI	RGATCY	2 cut(s)	4, 1250
MlsI	TGGCCA	1 cut(s)	1596
MluNI	TGGCCA	1 cut(s)	1596
MlyI	GAGTC	3 cut(s)	851, 1072, 1748
MmeI	TCCRAC	2 cut(s)	195, 1601
Mox20I	TGGCCA	1 cut(s)	1596
Mph1103I	ATGCAT	1 cut(s)	60
MroXI	GAANNNNTTC	1 cut(s)	371
MscI	TGGCCA	1 cut(s)	1596
MseI	TTAA	6 cut(s)	204, 543, 1167, 1571, 1742, 1806
Msp20I	TGGCCA	1 cut(s)	1596
MspA1I	CMGCKG	2 cut(s)	1817, 2148
MspI	CCGG	4 cut(s)	8, 595, 1802, 2087
MspR9I	CCNGG	6 cut(s)	661, 1137, 1218, 1231, 1766, 2087
Mva1269I	GAATGC	1 cut(s)	373
MvaI	CCWGG	5 cut(s)	661, 1137, 1218, 1231, 1766
MwoI	GCNNNNNNNGC	2 cut(s)	821, 1519
NciI	CCSGG	1 cut(s)	2087
NcoI	CCATGG	2 cut(s)	1073, 1876
NdeI	CATATG	1 cut(s)	622
NlaIV	GGNNCC	4 cut(s)	6, 24, 1071, 1443
NmuCI	GTSAC	3 cut(s)	10, 484, 523
NsiI	ATGCAT	1 cut(s)	60
NspI	RCATGY	2 cut(s)	970, 1064
NspV	TTCGAA	1 cut(s)	573
PaeI	GCATGC	2 cut(s)	970, 1064
PaeR7I	CTCGAG	1 cut(s)	85
PagI	TCATGA	3 cut(s)	387, 741, 1009
PceI	AGGCCT	1 cut(s)	1931
PctI	GAATGC	1 cut(s)	373
PdmI	GAANNNNTTC	1 cut(s)	371
PflMI	CCANNNNNTGG	1 cut(s)	1592
PfoI	TCCNGGA	4 cut(s)	659, 1216, 1764, 2085
PkrI	GCNGC	6 cut(s)	1173, 1663, 1666, 1816, 1819, 1949
PleI	GAGTC	3 cut(s)	851, 1072, 1748
PmaCI	CACGTG	1 cut(s)	560
PmlI	CACGTG	1 cut(s)	560
PpsI	GAGTC	3 cut(s)	851, 1072, 1748
Ppu21I	YACGTR	1 cut(s)	560
PpuMI	RGGWCCY	1 cut(s)	1720
PshBI	ATTAAT	2 cut(s)	543, 1742
Psp5II	RGGWCCY	1 cut(s)	1720
Psp6I	CCWGG	5 cut(s)	659, 1135, 1216, 1229, 1764
PspCI	CACGTG	1 cut(s)	560
PspGI	CCWGG	5 cut(s)	659, 1135, 1216, 1229, 1764
PspN4I	GGNNCC	4 cut(s)	6, 24, 1071, 1443
PspPI	GGNCC	2 cut(s)	1441, 1720
PspPPI	RGGWCCY	1 cut(s)	1720
PstI	CTGCAG	1 cut(s)	1669
PstNI	CAGNNNCTG	1 cut(s)	1817
PsuI	RGATCY	2 cut(s)	4, 1250
PvuII	CAGCTG	2 cut(s)	1817, 2148
RsaI	GTAC	6 cut(s)	151, 334, 435, 707, 1882, 1983
RsaNI	GTAC	6 cut(s)	150, 333, 434, 706, 1881, 1982
SaqAI	TTAA	6 cut(s)	204, 543, 1167, 1571, 1742, 1806
SatI	GCNGC	6 cut(s)	1172, 1662, 1665, 1815, 1818, 1948
Sau96I	GGNCC	2 cut(s)	1441, 1720
ScaI	AGTACT	1 cut(s)	334
SchI	GAGTC	3 cut(s)	851, 1072, 1748
ScrFI	CCNGG	6 cut(s)	661, 1137, 1218, 1231, 1766, 2087
SfaNI	GCATC	3 cut(s)	45, 802, 1023
SfcI	CTRYAG	2 cut(s)	417, 1665
Sfr274I	CTCGAG	1 cut(s)	85
SfuI	TTCGAA	1 cut(s)	573
SinI	GGWCC	1 cut(s)	1720
SlaI	CTCGAG	1 cut(s)	85
SmlI	CTYRAG	2 cut(s)	85, 951
SmoI	CTYRAG	2 cut(s)	85, 951
SpeI	ACTAGT	1 cut(s)	883
SphI	GCATGC	2 cut(s)	970, 1064
SseBI	AGGCCT	1 cut(s)	1931
SsiI	CCGC	4 cut(s)	824, 1354, 1522, 1820
SspI	AATATT	1 cut(s)	452
SspMI	CTAG	2 cut(s)	848, 884
StuI	AGGCCT	1 cut(s)	1931
StyD4I	CCNGG	6 cut(s)	659, 1135, 1216, 1229, 1764, 2085
StyI	CCWWGG	4 cut(s)	1073, 1704, 1876, 1932
TaaI	ACNGT	4 cut(s)	1043, 1321, 1943, 2056
TaiI	ACGT	2 cut(s)	155, 562
TaqI	TCGA	5 cut(s)	48, 86, 573, 1484, 2120
TaqII	GACCGA	1 cut(s)	891
TatI	WGTACW	2 cut(s)	332, 433
Tru1I	TTAA	6 cut(s)	204, 543, 1167, 1571, 1742, 1806
Tru9I	TTAA	6 cut(s)	204, 543, 1167, 1571, 1742, 1806
TscAI	CASTG	4 cut(s)	24, 1147, 1674, 1803
TseFI	GTSAC	3 cut(s)	10, 484, 523
TseI	GCWGC	6 cut(s)	1171, 1661, 1664, 1814, 1817, 1947
Tsp45I	GTSAC	3 cut(s)	10, 484, 523
TspGWI	ACGGA	2 cut(s)	284, 658
TspRI	CASTG	4 cut(s)	24, 1147, 1674, 1803
Van91I	CCANNNNNTGG	1 cut(s)	1592
VpaK11BI	GGWCC	1 cut(s)	1720
VspI	ATTAAT	2 cut(s)	543, 1742
XagI	CCTNNNNNAGG	2 cut(s)	88, 127
XapI	RAATTY	6 cut(s)	163, 512, 1212, 1525, 1909, 2048
XceI	RCATGY	2 cut(s)	970, 1064
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1852
XhoI	CTCGAG	1 cut(s)	85
XmnI	GAANNNNTTC	1 cut(s)	371
XspI	CTAG	2 cut(s)	848, 884
ZrmI	AGTACT	1 cut(s)	334
Zsp2I	ATGCAT	1 cut(s)	60

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.