MD03G1238800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014693
Physical Location & Seq
Reverse (-)
32395419 .. 32398103
2685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1238800.v1.1.491

Sequence Viewer

Length: 1362 bp
ATGGCTCATGAGGAAGAACAAACCCAAGACACTAGCACTGAAATTATTGGGTTGGGAAGCAATAACATCATCAGTAGTAGATCTTCATCCAAAAAAACAAAGCAGAAAAAAGTGCCACAGAGAGGACTTGGTGTTGCACAGCTTGAGAAAATCAGGCTTGAAGAACAGCAGAAGAAAGCAGCAGCAGCTGCAATTTTGTCCACCACCCCACCATCTTCTCTCTCATTACCAACAAAATCTTCTTCACATTCGTGTACTAGTTCGGTTCCGATCAGGCATTTTTATCAGCCTCCCTCTTCAATCCCATTTCCATCACTGGATCTGCCCTCCCCCAACTCGATTTTCCGTCCGCAGTTGATTCCACCGGCCCACAGCGGCGTTGATGGTAGGAAGCCAATGATCAGCAGTGGAACTTTTCCGTTTGGTGTGGGAAATAATAACAACAACAATAATATTAATGGTGGTGGGAATGGGAATCATGTTCCCAACTTGTGGAATCCATGTGAGTTTAATATTCTTGATCAGAAGGTGAGTTGTAATCCTGGGTTGGATCCTGGATTGGCATTCAGGTCTACTATGAATTTACCTTTTGAGTCCAGCAACAACAACCCCATTTGGCCGCTCTCCAATTTTCCAACAAGAACACAGCAATTTCATCACCAGCAGCCTCCTCCTCCACCGCCACCTCCTCCTCCTCCTTCAATGGTTAATCTGTCAACAGCAAATTCATCATCTGTACTAAATTTTCAGATAGAGCCCCCTTCAAACCAAAGCTATTACAGCAACTATACTCCTATGTGGCCGGATGAAGAAAAGATGGTTGGCATGAAGAGGCCATATCCATTTTCTCTAGAGAATCCACCTGTCCCCTCCATCAAGTTCCCTACATTTGTTGCTCCTCCTATAAGAGCCGATGAAGCAACTTCATGTGGGAACAGATCTGCCTTCAATTTTAATCCCGCAATCAAAACAAATTTCAGAGAAGGACCTTCATGCTCAACTTCAATTTCTGAGTCGAATTCAAGAAGCAGCATCAAAGAAAATGGGAGTGTCAATGGAGATTTTCTCACCCTAGCCCCTCCTTCAACTAGTTGGACATGTCCCAGCCCAAAGCTCAACCTCCCTCCATCTTATCTTGCGTTTCATAACCGTGAAATTCCGGATTTTGAATCATTTCACTATCAAGGGAATAATGCAGATGACACAATTTACCAGGCAGGACCAAGTGTTCCAAATCCACAACAAGCTTTCTACAGCTTCTTCCCACTAGCAAAGGCACAAAATGATAGGGCAGCGACCACTGTAAACAACAATTGCAATGGTGAAGTAGGAGAAAGTGTTGATCTCAATTTGAAGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NOZZLE

Protein Analysis

454

Amino Acids

49.37

Weight (kDa)

7.11

Isoelectric Point (pI)

62.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g18750 FvH4_3g18750 FvH4_3g18750
malus_domestica MD03G1238800.v1.1 MD11G1258600.v1.1
prunus_persica Prupe.4G171200_v2.0.a1
pyrus_communis pycom11g22840
rosa_chinensis RchiOBHm_Chr5g0031561
rosa_laevigata RLG00000033324
rosa_multiflora Rmu_sc0014784.1_g000002
rosa_roxburghii Rroxscaffold_1G00048740
rosa_rugosa Rorug05G0127000
rosa_samantha Rh5AG220200 Rh5BG219600 Rh5CG243000 Rh5DG224700
rosa_wichuraiana Rw5G020080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1360
AccB7I CCANNNNNTGG 1 cut(s) 492
AccBSI CCGCTC 1 cut(s) 622
AccI GTMKAC 1 cut(s) 572
AccIII TCCGGA 1 cut(s) 1159
AciI CCGC 5 cut(s) 350, 375, 620, 680, 960
AclWI GGATC 3 cut(s) 327, 545, 558
AcoI YGGCCR 2 cut(s) 617, 800
AcsI RAATTY 6 cut(s) 580, 724, 742, 973, 1018, 1155
AfaI GTAC 2 cut(s) 256, 738
AfiI CCNNNNNNNGG 2 cut(s) 122, 492
AflIII ACRYGT 1 cut(s) 1097
AhlI ACTAGT 2 cut(s) 257, 1088
AjnI CCWGG 3 cut(s) 541, 553, 1212
AjuI GAANNNNNNNTTGG 6 cut(s) 223, 255, 326, 358, 804, 836
AleI CACNNNNGTG 1 cut(s) 250
AloI GAACNNNNNNTCC 2 cut(s) 1212, 1244
AluBI AGCT 6 cut(s) 142, 188, 774, 1114, 1247, 1257
AluI AGCT 6 cut(s) 142, 188, 774, 1114, 1247, 1257
AlwI GGATC 3 cut(s) 327, 545, 558
AlwNI CAGNNNCTG 1 cut(s) 188
Aor13HI TCCGGA 1 cut(s) 1159
AoxI GGCC 4 cut(s) 366, 617, 800, 833
ApeKI GCWGC 7 cut(s) 179, 182, 185, 188, 664, 1029, 1292
ApoI RAATTY 6 cut(s) 580, 724, 742, 973, 1018, 1155
AseI ATTAAT 1 cut(s) 456
Asp700I GAANNNNTTC 1 cut(s) 1173
AspS9I GGNCC 3 cut(s) 367, 986, 1220
AsuHPI GGTGA 4 cut(s) 541, 650, 1060, 1334
AvaII GGWCC 2 cut(s) 986, 1220
BamHI GGATCC 1 cut(s) 550
BanII GRGCYC 1 cut(s) 759
BarI GAAGNNNNNNTAC 2 cut(s) 67, 99
BbvI GCAGC 7 cut(s) 175, 191, 194, 197, 676, 1041, 1304
BccI CCATC 6 cut(s) 220, 319, 377, 811, 881, 1135
BciT130I CCWGG 3 cut(s) 543, 555, 1214
BclI TGATCA 2 cut(s) 399, 520
BcuI ACTAGT 2 cut(s) 257, 1088
BfaI CTAG 6 cut(s) 33, 258, 851, 1073, 1089, 1268
BfmI CTRYAG 1 cut(s) 1252
BglII AGATCT 2 cut(s) 80, 938
BisI GCNGC 9 cut(s) 180, 183, 186, 189, 376, 620, 665, 1030, 1293
BlsI GCNGC 9 cut(s) 181, 184, 187, 190, 377, 621, 666, 1031, 1294
Bme1390I CCNGG 3 cut(s) 543, 555, 1214
Bme18I GGWCC 2 cut(s) 986, 1220
BmgT120I GGNCC 3 cut(s) 367, 986, 1220
BmiI GGNNCC 2 cut(s) 267, 552
BmrFI CCNGG 3 cut(s) 543, 555, 1214
BmsI GCATC 1 cut(s) 1041
BplI GAGNNNNNCTC 2 cut(s) 1050, 1082
BpuEI CTTGAG 1 cut(s) 164
BsaBI GATNNNNATC 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 542
BsaWI WCCGGW 1 cut(s) 1159
BsaXI ACNNNNNCTCC 2 cut(s) 1323, 1353
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 492
Bse118I RCCGGY 1 cut(s) 364
Bse1I ACTGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 1324
Bse8I GATNNNNATC 1 cut(s) 85
BseAI TCCGGA 1 cut(s) 1159
BseBI CCWGG 3 cut(s) 543, 555, 1214
BseDI CCNNGG 1 cut(s) 542
BseGI GGATG 2 cut(s) 86, 811
BseJI GATNNNNATC 1 cut(s) 85
BseLI CCNNNNNNNGG 2 cut(s) 122, 492
BseMI GCAATG 1 cut(s) 1324
BseMII CTCAG 1 cut(s) 1002
BseNI ACTGG 1 cut(s) 321
BseRI GAGGAG 6 cut(s) 660, 663, 678, 681, 684, 888
BseXI GCAGC 7 cut(s) 175, 191, 194, 197, 676, 1041, 1304
BseYI CCCAGC 1 cut(s) 1103
BshFI GGCC 4 cut(s) 368, 619, 802, 835
BsiSI CCGG 3 cut(s) 365, 803, 1160
BslFI GGGAC 2 cut(s) 851, 1086
BslI CCNNNNNNNGG 2 cut(s) 122, 492
BsmFI GGGAC 2 cut(s) 851, 1086
BsmI GAATGC 1 cut(s) 563
BsnI GGCC 4 cut(s) 368, 619, 802, 835
Bsp1286I GDGCHC 1 cut(s) 759
Bsp13I TCCGGA 1 cut(s) 1159
Bsp143I GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 938, 1342
BspACI CCGC 5 cut(s) 350, 375, 620, 680, 960
BspANI GGCC 4 cut(s) 368, 619, 802, 835
BspCNI CTCAG 1 cut(s) 1003
BspEI TCCGGA 1 cut(s) 1159
BspHI TCATGA 1 cut(s) 7
BspLI GGNNCC 2 cut(s) 267, 552
BspPI GGATC 3 cut(s) 327, 545, 558
BsrBI CCGCTC 1 cut(s) 622
BsrDI GCAATG 1 cut(s) 1324
BsrFI RCCGGY 1 cut(s) 364
BsrI ACTGG 1 cut(s) 321
BssAI RCCGGY 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 542
BssMI GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 938, 1342
Bst2UI CCWGG 3 cut(s) 543, 555, 1214
Bst4CI ACNGT 2 cut(s) 1151, 1303
Bst6I CTCTTC 2 cut(s) 301, 824
BstAPI GCANNNNNTGC 1 cut(s) 188
BstDEI CTNAG 1 cut(s) 1011
BstF5I GGATG 2 cut(s) 86, 811
BstKTI GATC 8 cut(s) 83, 273, 322, 402, 523, 553, 941, 1345
BstMBI GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 938, 1342
BstMWI GCNNNNNNNGC 4 cut(s) 185, 188, 780, 917
BstNI CCWGG 3 cut(s) 543, 555, 1214
BstNSI RCATGY 1 cut(s) 1101
BstSCI CCNGG 3 cut(s) 541, 553, 1212
BstSFI CTRYAG 1 cut(s) 1252
BstV1I GCAGC 7 cut(s) 175, 191, 194, 197, 676, 1041, 1304
BstX2I RGATCY 4 cut(s) 80, 319, 550, 938
BstYI RGATCY 4 cut(s) 80, 319, 550, 938
BsuRI GGCC 4 cut(s) 368, 619, 802, 835
BtsCI GGATG 2 cut(s) 86, 811
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 4 cut(s) 36, 314, 412, 1299
CaiI CAGNNNCTG 1 cut(s) 188
CciI TCATGA 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 364
Cfr13I GGNCC 3 cut(s) 367, 986, 1220
Csp6I GTAC 2 cut(s) 255, 737
CviAII CATG 7 cut(s) 8, 479, 501, 826, 927, 993, 1098
CviQI GTAC 2 cut(s) 255, 737
DdeI CTNAG 1 cut(s) 1011
DpnI GATC 8 cut(s) 82, 272, 321, 401, 522, 552, 940, 1344
DpnII GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 938, 1342
EaeI YGGCCR 2 cut(s) 617, 800
Eam1104I CTCTTC 2 cut(s) 301, 824
EarI CTCTTC 2 cut(s) 301, 824
Eco24I GRGCYC 1 cut(s) 759
Eco47I GGWCC 2 cut(s) 986, 1220
EcoO109I RGGNCCY 1 cut(s) 986
EcoRI GAATTC 1 cut(s) 1018
EcoRII CCWGG 3 cut(s) 541, 553, 1212
EcoT38I GRGCYC 1 cut(s) 759
FaeI CATG 7 cut(s) 11, 482, 504, 829, 930, 996, 1101
FaqI GGGAC 2 cut(s) 851, 1086
FatI CATG 7 cut(s) 7, 478, 500, 825, 926, 992, 1097
FauI CCCGC 1 cut(s) 967
FbaI TGATCA 2 cut(s) 399, 520
FblI GTMKAC 1 cut(s) 572
Fnu4HI GCNGC 9 cut(s) 180, 183, 186, 189, 376, 620, 665, 1030, 1293
FokI GGATG 2 cut(s) 73, 818
FriOI GRGCYC 1 cut(s) 759
Fsp4HI GCNGC 9 cut(s) 180, 183, 186, 189, 376, 620, 665, 1030, 1293
FspBI CTAG 6 cut(s) 33, 258, 851, 1073, 1089, 1268
GluI GCNGC 9 cut(s) 180, 183, 186, 189, 376, 620, 665, 1030, 1293
GsaI CCCAGC 1 cut(s) 1107
HaeIII GGCC 4 cut(s) 368, 619, 802, 835
HapII CCGG 3 cut(s) 365, 803, 1160
Hin1II CATG 7 cut(s) 11, 482, 504, 829, 930, 996, 1101
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HindIII AAGCTT 1 cut(s) 1245
HinfI GANTC 7 cut(s) 358, 475, 496, 593, 856, 1013, 1169
HpaII CCGG 3 cut(s) 365, 803, 1160
HphI GGTGA 4 cut(s) 541, 650, 1060, 1334
Hpy166II GTNNAC 5 cut(s) 201, 255, 573, 717, 1306
Hpy188I TCNGA 5 cut(s) 270, 525, 750, 980, 1012
Hpy188III TCNNGA 5 cut(s) 8, 518, 851, 1023, 1160
Hpy8I GTNNAC 5 cut(s) 201, 255, 573, 717, 1306
HpyAV CCTTC 7 cut(s) 520, 708, 771, 955, 977, 999, 1092
HpyCH4III ACNGT 2 cut(s) 1151, 1303
HpyCH4V TGCA 4 cut(s) 137, 191, 1196, 1317
HpyF10VI GCNNNNNNNGC 4 cut(s) 185, 188, 780, 917
HpyF3I CTNAG 1 cut(s) 1011
Hsp92II CATG 7 cut(s) 11, 482, 504, 829, 930, 996, 1101
Kpn2I TCCGGA 1 cut(s) 1159
Ksp22I TGATCA 2 cut(s) 399, 520
Kzo9I GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 938, 1342
LmnI GCTCC 1 cut(s) 901
Lsp1109I GCAGC 7 cut(s) 175, 191, 194, 197, 676, 1041, 1304
LweI GCATC 1 cut(s) 1041
MaeI CTAG 6 cut(s) 33, 258, 851, 1073, 1089, 1268
MalI GATC 8 cut(s) 82, 272, 321, 401, 522, 552, 940, 1344
MbiI CCGCTC 1 cut(s) 622
MboI GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 938, 1342
MfeI CAATTG 1 cut(s) 1312
MflI RGATCY 4 cut(s) 80, 319, 550, 938
MhlI GDGCHC 1 cut(s) 759
MlyI GAGTC 2 cut(s) 602, 1022
MmeI TCCRAC 3 cut(s) 528, 659, 1073
MroI TCCGGA 1 cut(s) 1159
MroXI GAANNNNTTC 1 cut(s) 1173
MseI TTAA 4 cut(s) 456, 510, 708, 954
MslI CAYNNNNRTG 2 cut(s) 250, 1149
MspA1I CMGCKG 2 cut(s) 188, 375
MspI CCGG 3 cut(s) 365, 803, 1160
MspR9I CCNGG 3 cut(s) 543, 555, 1214
MunI CAATTG 1 cut(s) 1312
Mva1269I GAATGC 1 cut(s) 563
MvaI CCWGG 3 cut(s) 543, 555, 1214
MwoI GCNNNNNNNGC 4 cut(s) 185, 188, 780, 917
NdeII GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 938, 1342
NlaIII CATG 7 cut(s) 11, 482, 504, 829, 930, 996, 1101
NlaIV GGNNCC 2 cut(s) 267, 552
NspI RCATGY 1 cut(s) 1101
OliI CACNNNNGTG 1 cut(s) 250
PagI TCATGA 1 cut(s) 7
PciI ACATGT 1 cut(s) 1097
PctI GAATGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 1173
PfeI GAWTC 5 cut(s) 358, 475, 496, 856, 1169
PflMI CCANNNNNTGG 1 cut(s) 492
PfoI TCCNGGA 1 cut(s) 553
PkrI GCNGC 9 cut(s) 181, 184, 187, 190, 377, 621, 666, 1031, 1294
PleI GAGTC 2 cut(s) 601, 1021
PpsI GAGTC 2 cut(s) 601, 1021
PpuMI RGGWCCY 1 cut(s) 986
PscI ACATGT 1 cut(s) 1097
PshBI ATTAAT 1 cut(s) 456
PsiI TTATAA 1 cut(s) 1360
Psp5II RGGWCCY 1 cut(s) 986
Psp6I CCWGG 3 cut(s) 541, 553, 1212
PspFI CCCAGC 1 cut(s) 1103
PspGI CCWGG 3 cut(s) 541, 553, 1212
PspN4I GGNNCC 2 cut(s) 267, 552
PspPI GGNCC 3 cut(s) 367, 986, 1220
PspPPI RGGWCCY 1 cut(s) 986
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 4 cut(s) 80, 319, 550, 938
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 256, 738
RsaNI GTAC 2 cut(s) 255, 737
RseI CAYNNNNRTG 2 cut(s) 250, 1149
SaqAI TTAA 4 cut(s) 456, 510, 708, 954
SatI GCNGC 9 cut(s) 180, 183, 186, 189, 376, 620, 665, 1030, 1293
Sau3AI GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 938, 1342
Sau96I GGNCC 3 cut(s) 367, 986, 1220
SchI GAGTC 2 cut(s) 602, 1022
ScrFI CCNGG 3 cut(s) 543, 555, 1214
SduI GDGCHC 1 cut(s) 759
SfaNI GCATC 1 cut(s) 1041
SfcI CTRYAG 1 cut(s) 1252
SinI GGWCC 2 cut(s) 986, 1220
SmiMI CAYNNNNRTG 2 cut(s) 250, 1149
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
SpeI ACTAGT 2 cut(s) 257, 1088
SsiI CCGC 5 cut(s) 350, 375, 620, 680, 960
SspI AATATT 2 cut(s) 454, 514
SspMI CTAG 6 cut(s) 33, 258, 851, 1073, 1089, 1268
StyD4I CCNGG 3 cut(s) 541, 553, 1212
TaaI ACNGT 2 cut(s) 1151, 1303
TaqI TCGA 2 cut(s) 338, 1016
TatI WGTACW 2 cut(s) 254, 736
TauI GCSGC 2 cut(s) 378, 622
TfiI GAWTC 5 cut(s) 358, 475, 496, 856, 1169
Tru1I TTAA 4 cut(s) 456, 510, 708, 954
Tru9I TTAA 4 cut(s) 456, 510, 708, 954
TscAI CASTG 4 cut(s) 43, 321, 412, 1306
TseI GCWGC 7 cut(s) 179, 182, 185, 188, 664, 1029, 1292
TspGWI ACGGA 2 cut(s) 335, 408
TspRI CASTG 4 cut(s) 43, 321, 412, 1306
Van91I CCANNNNNTGG 1 cut(s) 492
VpaK11BI GGWCC 2 cut(s) 986, 1220
VspI ATTAAT 1 cut(s) 456
XapI RAATTY 6 cut(s) 580, 724, 742, 973, 1018, 1155
XbaI TCTAGA 1 cut(s) 850
XceI RCATGY 1 cut(s) 1101
XmiI GTMKAC 1 cut(s) 572
XmnI GAANNNNTTC 1 cut(s) 1173
XspI CTAG 6 cut(s) 33, 258, 851, 1073, 1089, 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.