MD11G1258600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0014693
Physical Location & Seq
Reverse (-)
37216903 .. 37219228
2326 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1258600.v1.1.491

Sequence Viewer

Length: 1347 bp
ATGGCTCATGAGGAAGAACAAACCCAAAACACTAGCAATGGAATTATTGGGTTGGGAAGCAATAACATCATCAGTAGCAGATCTTCATCCAAAAAAACAAAGCAGAAAAAAGTGCCACAGAGAGGACTTGGTGTTGCACAGCTTGAGAAAATCAGGCTTGAAGAACAGCAGAAGAAAGCAGCTGCTGCGGCAATTTTGTCCACCACCCCACCATCTTCTCTCTCATCACCAACTAAATCTACTTCATCTTCATGTCCTAGTTCGGTTCCGATCAGGCATTTTTATCAGCCTCCCTCTTCAATCCCATTCCCATCACTGGATCTGCCTTCTCCAAACTCAATTTTCCAGCCGCCGTTGATTCCACCGGCCCACAGCGGCGTTGATGGTAGAAAGCTAATGATCAGCAGTGGCACTGTTCCATTTGGAATGAGAAATAGTTACAACAATAATAATATTAATGGTTGTGGGAATGGGAATCATGTTCCCAACTTGTGGAATCCGTGTGAGTTCAATGTTCTTGATCACAAGGAGAGTTCTAATCCTGGGTTGGATCCTGGATTGGCATTCAGGTCTAATTTGAATGTGCCTTTCGAGTCCAACAACAACAACCCCATTAGGGCTCTCTCCAATTTTCCAACAAGAACACACCAATTTCATCAACAGCACCCTCCTCCACCGCCACCGCCACCACCATCATCTCCTCCTCCTTCAATGGTGAATGTGTCAACAGCAACTTCAACATCATCTGTACTAAATTTTCAGATAGAGCCCCCTTCAAACCAAAGCTATTACAGCAACTATGCTCCTATGTGGCCGGATGAAGAAAAGATGGTTGGCATGAAGAGGCCATATCCATTTTCTCTAGAGAACCCATCAGTCCCCTCCATCAACTTCAAGTTCCCTACCTTTGTTGCTCCACCTATAAGAACAGACGAAGGAACTTCATGTGGGAACAGGTCCCCCTTCAATTTTGATCCAGCAATCAACCCAAATTTCAGAAGGCCCTTCATGGTCAAATTCAATTTCAGAGTCAAATTCGAGAAGCAGCATCAAAGAAAATGGGAGTGTCAATGGAGATTTTCTCACCCTAGCCCAAAACTCAAACTCCCTCCATCTTATCTTGCGTTTCATAACCGCGAAGTTCCGGATTTCGAATCATTCCCCTATCAAGGGAATAACGCAGATGACACATATTACCAGCCAGGACCAAGTATTCCAAATCCACAACAAGCTTTCTACAGCTTCTTCCCACCAGCAAAGTCACAAAATGGTAGGGCAGCAACCACTCTAAACAACAATTGTAATGGTGAAGTAGGAGAAAGTGTTGATCTCAAATTGAGGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NOZZLE

Protein Analysis

449

Amino Acids

49.74

Weight (kDa)

9.33

Isoelectric Point (pI)

63.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g18750 FvH4_3g18750 FvH4_3g18750
malus_domestica MD03G1238800.v1.1 MD11G1258600.v1.1
prunus_persica Prupe.4G171200_v2.0.a1
pyrus_communis pycom11g22840
rosa_chinensis RchiOBHm_Chr5g0031561
rosa_laevigata RLG00000033324
rosa_multiflora Rmu_sc0014784.1_g000002
rosa_roxburghii Rroxscaffold_1G00048740
rosa_rugosa Rorug05G0127000
rosa_samantha Rh5AG220200 Rh5BG219600 Rh5CG243000 Rh5DG224700
rosa_wichuraiana Rw5G020080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1345
AccB7I CCANNNNNTGG 1 cut(s) 492
AccII CGCG 1 cut(s) 1137
AccIII TCCGGA 1 cut(s) 1144
AciI CCGC 6 cut(s) 188, 350, 375, 677, 683, 1135
AclWI GGATC 4 cut(s) 327, 545, 558, 968
AcoI YGGCCR 1 cut(s) 812
AcsI RAATTY 4 cut(s) 754, 991, 1016, 1034
AfaI GTAC 1 cut(s) 750
AfiI CCNNNNNNNGG 6 cut(s) 122, 316, 492, 616, 1169, 1170
AjnI CCWGG 3 cut(s) 541, 553, 1201
AjuI GAANNNNNNNTTGG 2 cut(s) 816, 848
AluBI AGCT 6 cut(s) 142, 182, 394, 786, 1232, 1242
AluI AGCT 6 cut(s) 142, 182, 394, 786, 1232, 1242
AlwI GGATC 4 cut(s) 327, 545, 558, 968
AlwNI CAGNNNCTG 1 cut(s) 185
Aor13HI TCCGGA 1 cut(s) 1144
AoxI GGCC 4 cut(s) 366, 812, 845, 1001
ApeKI GCWGC 5 cut(s) 179, 182, 185, 1045, 1277
ApoI RAATTY 4 cut(s) 754, 991, 1016, 1034
AseI ATTAAT 1 cut(s) 456
AspS9I GGNCC 4 cut(s) 367, 957, 1002, 1205
AsuHPI GGTGA 4 cut(s) 219, 727, 1076, 1319
AsuII TTCGAA 1 cut(s) 1152
AvaII GGWCC 2 cut(s) 957, 1205
BamHI GGATCC 1 cut(s) 550
BanII GRGCYC 2 cut(s) 622, 771
BarI GAAGNNNNNNTAC 2 cut(s) 67, 99
BbvI GCAGC 5 cut(s) 169, 172, 191, 1057, 1289
BccI CCATC 8 cut(s) 220, 319, 377, 700, 823, 880, 893, 1120
BceAI ACGGC 1 cut(s) 337
BciT130I CCWGG 3 cut(s) 543, 555, 1203
BclI TGATCA 2 cut(s) 399, 520
BfaI CTAG 4 cut(s) 33, 258, 863, 1089
BfmI CTRYAG 1 cut(s) 1237
BglII AGATCT 1 cut(s) 80
BisI GCNGC 8 cut(s) 180, 183, 186, 189, 350, 376, 1046, 1278
BlsI GCNGC 8 cut(s) 181, 184, 187, 190, 351, 377, 1047, 1279
Bme1390I CCNGG 3 cut(s) 543, 555, 1203
Bme18I GGWCC 2 cut(s) 957, 1205
BmgT120I GGNCC 4 cut(s) 367, 957, 1002, 1205
BmiI GGNNCC 3 cut(s) 267, 552, 959
BmrFI CCNGG 3 cut(s) 543, 555, 1203
BmsI GCATC 1 cut(s) 1057
BplI GAGNNNNNCTC 2 cut(s) 1066, 1098
Bpu14I TTCGAA 1 cut(s) 1152
BpuEI CTTGAG 1 cut(s) 164
BsaBI GATNNNNATC 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 542
BsaWI WCCGGW 1 cut(s) 1144
BsaXI ACNNNNNCTCC 4 cut(s) 1089, 1119, 1308, 1338
Bsc4I CCNNNNNNNGG 6 cut(s) 122, 316, 492, 616, 1169, 1170
Bse118I RCCGGY 1 cut(s) 364
Bse1I ACTGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 43
Bse8I GATNNNNATC 1 cut(s) 85
BseAI TCCGGA 1 cut(s) 1144
BseBI CCWGG 3 cut(s) 543, 555, 1203
BseDI CCNNGG 1 cut(s) 542
BseGI GGATG 2 cut(s) 86, 823
BseJI GATNNNNATC 1 cut(s) 85
BseLI CCNNNNNNNGG 6 cut(s) 122, 316, 492, 616, 1169, 1170
BseMI GCAATG 1 cut(s) 43
BseNI ACTGG 1 cut(s) 321
BseRI GAGGAG 3 cut(s) 660, 690, 693
BseXI GCAGC 5 cut(s) 169, 172, 191, 1057, 1289
Bsh1236I CGCG 1 cut(s) 1137
BshFI GGCC 4 cut(s) 368, 814, 847, 1003
BsiSI CCGG 3 cut(s) 365, 815, 1145
BslFI GGGAC 2 cut(s) 863, 943
BslI CCNNNNNNNGG 6 cut(s) 122, 316, 492, 616, 1169, 1170
BsmFI GGGAC 2 cut(s) 863, 943
BsmI GAATGC 1 cut(s) 563
BsnI GGCC 4 cut(s) 368, 814, 847, 1003
Bsp119I TTCGAA 1 cut(s) 1152
Bsp1286I GDGCHC 2 cut(s) 622, 771
Bsp13I TCCGGA 1 cut(s) 1144
Bsp143I GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 973, 1327
BspACI CCGC 6 cut(s) 188, 350, 375, 677, 683, 1135
BspANI GGCC 4 cut(s) 368, 814, 847, 1003
BspEI TCCGGA 1 cut(s) 1144
BspFNI CGCG 1 cut(s) 1137
BspHI TCATGA 1 cut(s) 7
BspLI GGNNCC 3 cut(s) 267, 552, 959
BspPI GGATC 4 cut(s) 327, 545, 558, 968
BspT104I TTCGAA 1 cut(s) 1152
BsrDI GCAATG 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 364
BsrI ACTGG 1 cut(s) 321
BssAI RCCGGY 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 542
BssMI GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 973, 1327
Bst2UI CCWGG 3 cut(s) 543, 555, 1203
Bst4CI ACNGT 1 cut(s) 415
Bst6I CTCTTC 2 cut(s) 301, 836
BstAPI GCANNNNNTGC 1 cut(s) 185
BstBI TTCGAA 1 cut(s) 1152
BstF5I GGATG 2 cut(s) 86, 823
BstFNI CGCG 1 cut(s) 1137
BstKTI GATC 8 cut(s) 83, 273, 322, 402, 523, 553, 976, 1330
BstMBI GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 973, 1327
BstMWI GCNNNNNNNGC 3 cut(s) 185, 188, 792
BstNI CCWGG 3 cut(s) 543, 555, 1203
BstSCI CCNGG 3 cut(s) 541, 553, 1201
BstSFI CTRYAG 1 cut(s) 1237
BstUI CGCG 1 cut(s) 1137
BstV1I GCAGC 5 cut(s) 169, 172, 191, 1057, 1289
BstX2I RGATCY 3 cut(s) 80, 319, 550
BstYI RGATCY 3 cut(s) 80, 319, 550
BsuRI GGCC 4 cut(s) 368, 814, 847, 1003
BtsCI GGATG 2 cut(s) 86, 823
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 3 cut(s) 314, 411, 412
CaiI CAGNNNCTG 1 cut(s) 185
CciI TCATGA 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 364
Cfr13I GGNCC 4 cut(s) 367, 957, 1002, 1205
Csp6I GTAC 1 cut(s) 749
CviAII CATG 6 cut(s) 8, 252, 479, 838, 945, 1009
CviQI GTAC 1 cut(s) 749
DpnI GATC 8 cut(s) 82, 272, 321, 401, 522, 552, 975, 1329
DpnII GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 973, 1327
EaeI YGGCCR 1 cut(s) 812
Eam1104I CTCTTC 2 cut(s) 301, 836
EarI CTCTTC 2 cut(s) 301, 836
Eco24I GRGCYC 2 cut(s) 622, 771
Eco47I GGWCC 2 cut(s) 957, 1205
EcoO109I RGGNCCY 2 cut(s) 957, 1002
EcoRII CCWGG 3 cut(s) 541, 553, 1201
EcoT38I GRGCYC 2 cut(s) 622, 771
FaeI CATG 6 cut(s) 11, 255, 482, 841, 948, 1012
FaqI GGGAC 2 cut(s) 863, 943
FatI CATG 6 cut(s) 7, 251, 478, 837, 944, 1008
FbaI TGATCA 2 cut(s) 399, 520
Fnu4HI GCNGC 8 cut(s) 180, 183, 186, 189, 350, 376, 1046, 1278
FokI GGATG 2 cut(s) 73, 830
FriOI GRGCYC 2 cut(s) 622, 771
Fsp4HI GCNGC 8 cut(s) 180, 183, 186, 189, 350, 376, 1046, 1278
FspBI CTAG 4 cut(s) 33, 258, 863, 1089
GluI GCNGC 8 cut(s) 180, 183, 186, 189, 350, 376, 1046, 1278
HaeIII GGCC 4 cut(s) 368, 814, 847, 1003
HapII CCGG 3 cut(s) 365, 815, 1145
Hin1II CATG 6 cut(s) 11, 255, 482, 841, 948, 1012
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HindIII AAGCTT 1 cut(s) 1230
HinfI GANTC 6 cut(s) 358, 475, 496, 593, 1029, 1154
HpaII CCGG 3 cut(s) 365, 815, 1145
HphI GGTGA 4 cut(s) 219, 727, 1076, 1319
Hpy166II GTNNAC 2 cut(s) 201, 726
Hpy188I TCNGA 4 cut(s) 270, 762, 998, 1028
Hpy188III TCNNGA 5 cut(s) 8, 518, 863, 1039, 1145
Hpy8I GTNNAC 2 cut(s) 201, 726
HpyAV CCTTC 7 cut(s) 336, 717, 783, 929, 973, 993, 1015
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4V TGCA 1 cut(s) 137
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 188, 792
Hsp92II CATG 6 cut(s) 11, 255, 482, 841, 948, 1012
Kpn2I TCCGGA 1 cut(s) 1144
Ksp22I TGATCA 2 cut(s) 399, 520
Kzo9I GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 973, 1327
LmnI GCTCC 2 cut(s) 808, 919
Lsp1109I GCAGC 5 cut(s) 169, 172, 191, 1057, 1289
LweI GCATC 1 cut(s) 1057
MaeI CTAG 4 cut(s) 33, 258, 863, 1089
MaeIII GTNAC 2 cut(s) 437, 1260
MalI GATC 8 cut(s) 82, 272, 321, 401, 522, 552, 975, 1329
MboI GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 973, 1327
MfeI CAATTG 1 cut(s) 1297
MflI RGATCY 3 cut(s) 80, 319, 550
MhlI GDGCHC 2 cut(s) 622, 771
MlyI GAGTC 2 cut(s) 602, 1038
MmeI TCCRAC 3 cut(s) 528, 621, 659
MroI TCCGGA 1 cut(s) 1144
MseI TTAA 1 cut(s) 456
MslI CAYNNNNRTG 1 cut(s) 250
MspA1I CMGCKG 2 cut(s) 182, 375
MspI CCGG 3 cut(s) 365, 815, 1145
MspR9I CCNGG 3 cut(s) 543, 555, 1203
MunI CAATTG 1 cut(s) 1297
Mva1269I GAATGC 1 cut(s) 563
MvaI CCWGG 3 cut(s) 543, 555, 1203
MvnI CGCG 1 cut(s) 1137
MwoI GCNNNNNNNGC 3 cut(s) 185, 188, 792
NdeII GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 973, 1327
NlaIII CATG 6 cut(s) 11, 255, 482, 841, 948, 1012
NlaIV GGNNCC 3 cut(s) 267, 552, 959
NmuCI GTSAC 1 cut(s) 1260
NspV TTCGAA 1 cut(s) 1152
PagI TCATGA 1 cut(s) 7
PctI GAATGC 1 cut(s) 563
PfeI GAWTC 4 cut(s) 358, 475, 496, 1154
PflMI CCANNNNNTGG 1 cut(s) 492
PfoI TCCNGGA 1 cut(s) 553
PkrI GCNGC 8 cut(s) 181, 184, 187, 190, 351, 377, 1047, 1279
PleI GAGTC 2 cut(s) 601, 1037
PpsI GAGTC 2 cut(s) 601, 1037
PpuMI RGGWCCY 1 cut(s) 957
PshBI ATTAAT 1 cut(s) 456
PsiI TTATAA 1 cut(s) 1345
Psp5II RGGWCCY 1 cut(s) 957
Psp6I CCWGG 3 cut(s) 541, 553, 1201
PspGI CCWGG 3 cut(s) 541, 553, 1201
PspN4I GGNNCC 3 cut(s) 267, 552, 959
PspPI GGNCC 4 cut(s) 367, 957, 1002, 1205
PspPPI RGGWCCY 1 cut(s) 957
PstNI CAGNNNCTG 1 cut(s) 185
PsuI RGATCY 3 cut(s) 80, 319, 550
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 1 cut(s) 750
RsaNI GTAC 1 cut(s) 749
RseI CAYNNNNRTG 1 cut(s) 250
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 8 cut(s) 180, 183, 186, 189, 350, 376, 1046, 1278
Sau3AI GATC 8 cut(s) 80, 270, 319, 399, 520, 550, 973, 1327
Sau96I GGNCC 4 cut(s) 367, 957, 1002, 1205
SchI GAGTC 2 cut(s) 602, 1038
ScrFI CCNGG 3 cut(s) 543, 555, 1203
SduI GDGCHC 2 cut(s) 622, 771
SfaNI GCATC 1 cut(s) 1057
SfcI CTRYAG 1 cut(s) 1237
SfuI TTCGAA 1 cut(s) 1152
SinI GGWCC 2 cut(s) 957, 1205
SmiMI CAYNNNNRTG 1 cut(s) 250
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
SsiI CCGC 6 cut(s) 188, 350, 375, 677, 683, 1135
SspI AATATT 1 cut(s) 454
SspMI CTAG 4 cut(s) 33, 258, 863, 1089
StyD4I CCNGG 3 cut(s) 541, 553, 1201
TaaI ACNGT 1 cut(s) 415
TaqI TCGA 3 cut(s) 591, 1038, 1152
TatI WGTACW 1 cut(s) 748
TauI GCSGC 3 cut(s) 191, 352, 378
TfiI GAWTC 4 cut(s) 358, 475, 496, 1154
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 3 cut(s) 321, 412, 418
TseFI GTSAC 1 cut(s) 1260
TseI GCWGC 5 cut(s) 179, 182, 185, 1045, 1277
Tsp45I GTSAC 1 cut(s) 1260
TspDTI ATGAA 9 cut(s) 75, 234, 240, 644, 834, 854, 933, 997, 1118
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 3 cut(s) 321, 412, 418
Van91I CCANNNNNTGG 1 cut(s) 492
VpaK11BI GGWCC 2 cut(s) 957, 1205
VspI ATTAAT 1 cut(s) 456
XapI RAATTY 4 cut(s) 754, 991, 1016, 1034
XbaI TCTAGA 1 cut(s) 862
XspI CTAG 4 cut(s) 33, 258, 863, 1089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.