Prupe.4G171200_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0014693
Physical Location & Seq
Forward (+)
10116204 .. 10120142
3939 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G171200.1

Sequence Viewer

Length: 1347 bp
ATGGCTCAAGAAGACCAAACTCAAAAGTGTAGCAATACTAGTAGTGGTGCTAGTCTTGGCTTTGCAAGCATTACTAGATCTTCATCCAAAAAAACCAAGCAAAAAAAAGTGCCACAGAGAGGACTTGGTGTGGCACAGCTTGAGAAAATCAGGCTTGAAGATCAGCAAAAGAAAGCAGCAGCTGCTGTGATTTTGTCAACCCCATCTTCTTTATCCTCACCGACCAAATCCACATCCTCATCATGTCCTTCTGTCCCAATTAGACATTTTTATCACTCTAATCATAATCAACCACCCTCTTCAATCCCATTTCCTTCTCTACCATCACTAGATCTACCTTCACCAAACTCAATTTTCAGGCCACCATTAATTCCAACCCAGAACATTGATGTAATTAGGAAGCCAATTAGCACTGTTCCATTGGGAAATAACAATAATAATAATGGTGGTCATGGGTTTGAAATTCAGGGACATGGCAATAATAATGTTCCTAACTTGTGGAACCCTTGTGACTTCAATCTTGATCAGAAGGAGAGTTCTAATTCTGGGATTGATCCCGGATTGGCGTTTCGGTCTACTTTGAATTTGCCTTTTGAGTCCAACAACCCCATTTGGCCTCTCACCAATGTGCCACAAAGAACACAACCATATCATCAGCAGCCTCCTCCTCCTCCTCCTTCAATGGTGAATGTGTCATCAGCAACTTCATCATCATCTGTACTAAACTTTCAGATAGAGCCCCCTTCAAACCAAAGCTATTACAGCAACTATACACCTATGTGGCCGGATGAAGAAAAGATGGTTGGCATGAAGAGGCCATACCCCTTTTCTCTAGACAATCCACCAGGGCCCTCCATCAACTTTAAATTCCCTACTTTTGTTGCTCCTATGAGAGCAGATGAAGCAAGTTCATGTGGGAACAGATACACCTTCAATTTGGAGCCTTCAGGCAACCCAATTTTCAGAGAAGCCCCTTCATGCTCAACTTCAATTTCGGAGTCGAATTCAAGGAACAGCATTAAAGAAAATGGGAGTGTCAATGGAGATTTTCTCACCTTAGCCCCTCCTACAACCAGTAGTAGGACTTGTCCCAGCCCAAAGTTGAACCTCCCTTCAACTTATCTAGCGTTCCATAACCGCGAAGTCCCTGAGTTTGAATCATTTTCTTATCAAGGCAATGCAGAGGACACAAATTTCCAGCCAGGACCAAGCTTTTCACATCCACAACAAGCTTTCTACAGCTTCTTCCCACCAGCAAAGGCACAAATTGGAAGAGCAGCAACCACTATAAACAACAATTGTAATGGTGAACTAGGAGAAAGTGTGGATCTCAATTTGAAGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NOZZLE

Protein Analysis

449

Amino Acids

48.87

Weight (kDa)

8.1

Isoelectric Point (pI)

56.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g18750 FvH4_3g18750 FvH4_3g18750
malus_domestica MD03G1238800.v1.1 MD11G1258600.v1.1
prunus_persica Prupe.4G171200_v2.0.a1
pyrus_communis pycom11g22840
rosa_chinensis RchiOBHm_Chr5g0031561
rosa_laevigata RLG00000033324
rosa_multiflora Rmu_sc0014784.1_g000002
rosa_roxburghii Rroxscaffold_1G00048740
rosa_rugosa Rorug05G0127000
rosa_samantha Rh5AG220200 Rh5BG219600 Rh5CG243000 Rh5DG224700
rosa_wichuraiana Rw5G020080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1345
AccI GTMKAC 1 cut(s) 575
AccII CGCG 1 cut(s) 1140
AciI CCGC 1 cut(s) 1138
AclWI GGATC 2 cut(s) 548, 1335
AcoI YGGCCR 1 cut(s) 782
AcsI RAATTY 5 cut(s) 462, 583, 866, 1003, 1192
AcuI CTGAAG 1 cut(s) 930
AfaI GTAC 1 cut(s) 720
AfiI CCNNNNNNNGG 2 cut(s) 119, 1080
AhlI ACTAGT 1 cut(s) 38
AjnI CCWGG 2 cut(s) 844, 1201
AjuI GAANNNNNNNTTGG 2 cut(s) 786, 818
AleI CACNNNNGTG 2 cut(s) 626, 778
AluBI AGCT 6 cut(s) 139, 182, 756, 1212, 1232, 1242
AluI AGCT 6 cut(s) 139, 182, 756, 1212, 1232, 1242
AlwI GGATC 2 cut(s) 548, 1335
AlwNI CAGNNNCTG 2 cut(s) 182, 185
AoxI GGCC 5 cut(s) 359, 614, 782, 815, 848
ApaI GGGCCC 1 cut(s) 852
ApeKI GCWGC 5 cut(s) 176, 179, 182, 658, 1277
ApoI RAATTY 5 cut(s) 462, 583, 866, 1003, 1192
AseI ATTAAT 1 cut(s) 368
AspS9I GGNCC 3 cut(s) 848, 849, 1205
AsuC2I CCSGG 1 cut(s) 558
AsuHPI GGTGA 6 cut(s) 210, 333, 613, 697, 1045, 1319
AvaII GGWCC 1 cut(s) 1205
BaeGI GKGCMC 1 cut(s) 852
BanII GRGCYC 2 cut(s) 741, 852
BarI GAAGNNNNNNTAC 2 cut(s) 803, 835
BbsI GAAGAC 1 cut(s) 18
BbvI GCAGC 5 cut(s) 169, 188, 191, 670, 1289
BccI CCATC 4 cut(s) 211, 331, 793, 863
BciT130I CCWGG 2 cut(s) 846, 1203
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 1 cut(s) 558
BcuI ACTAGT 1 cut(s) 38
BfaI CTAG 7 cut(s) 39, 51, 75, 329, 833, 1124, 1313
BfmI CTRYAG 1 cut(s) 1237
BglII AGATCT 2 cut(s) 77, 331
BisI GCNGC 5 cut(s) 177, 180, 183, 659, 1278
BlsI GCNGC 5 cut(s) 178, 181, 184, 660, 1279
Bme1390I CCNGG 3 cut(s) 558, 846, 1203
Bme18I GGWCC 1 cut(s) 1205
BmgT120I GGNCC 3 cut(s) 848, 849, 1205
BmiI GGNNCC 3 cut(s) 503, 850, 942
BmrFI CCNGG 3 cut(s) 558, 846, 1203
BpiI GAAGAC 1 cut(s) 18
BplI GAGNNNNNCTC 2 cut(s) 1035, 1067
Bpu10I CCTNAGC 1 cut(s) 1057
BpuEI CTTGAG 1 cut(s) 161
BpuMI CCSGG 1 cut(s) 558
BsaBI GATNNNNATC 1 cut(s) 82
BsaJI CCNNGG 1 cut(s) 845
BsaXI ACNNNNNCTCC 2 cut(s) 1308, 1338
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 1080
Bse1I ACTGG 1 cut(s) 1074
Bse3DI GCAATG 1 cut(s) 1183
Bse8I GATNNNNATC 1 cut(s) 82
BseBI CCWGG 2 cut(s) 846, 1203
BseDI CCNNGG 1 cut(s) 845
BseGI GGATG 4 cut(s) 83, 233, 793, 1219
BseJI GATNNNNATC 1 cut(s) 82
BseLI CCNNNNNNNGG 2 cut(s) 119, 1080
BseMI GCAATG 1 cut(s) 1183
BseMII CTCAG 1 cut(s) 1140
BseNI ACTGG 1 cut(s) 1074
BseRI GAGGAG 4 cut(s) 654, 657, 660, 663
BseSI GKGCMC 1 cut(s) 852
BseXI GCAGC 5 cut(s) 169, 188, 191, 670, 1289
BseYI CCCAGC 1 cut(s) 1091
Bsh1236I CGCG 1 cut(s) 1140
BshFI GGCC 5 cut(s) 361, 616, 784, 817, 850
BsiSI CCGG 2 cut(s) 558, 785
BslFI GGGAC 4 cut(s) 239, 483, 1074, 1130
BslI CCNNNNNNNGG 2 cut(s) 119, 1080
BsmFI GGGAC 4 cut(s) 239, 483, 1074, 1130
BsnI GGCC 5 cut(s) 361, 616, 784, 817, 850
Bsp120I GGGCCC 1 cut(s) 848
Bsp1286I GDGCHC 2 cut(s) 741, 852
Bsp143I GATC 6 cut(s) 77, 160, 331, 523, 553, 1327
BspACI CCGC 1 cut(s) 1138
BspANI GGCC 5 cut(s) 361, 616, 784, 817, 850
BspCNI CTCAG 1 cut(s) 1141
BspFNI CGCG 1 cut(s) 1140
BspLI GGNNCC 3 cut(s) 503, 850, 942
BspPI GGATC 2 cut(s) 548, 1335
BspQI GCTCTTC 1 cut(s) 1267
BsrDI GCAATG 1 cut(s) 1183
BsrI ACTGG 1 cut(s) 1074
BssECI CCNNGG 1 cut(s) 845
BssMI GATC 6 cut(s) 77, 160, 331, 523, 553, 1327
Bst2UI CCWGG 2 cut(s) 846, 1203
Bst4CI ACNGT 1 cut(s) 415
Bst6I CTCTTC 3 cut(s) 304, 806, 1267
BstAPI GCANNNNNTGC 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 67
BstDEI CTNAG 2 cut(s) 1057, 1149
BstF5I GGATG 4 cut(s) 83, 233, 793, 1219
BstFNI CGCG 1 cut(s) 1140
BstKTI GATC 6 cut(s) 80, 163, 334, 526, 556, 1330
BstMBI GATC 6 cut(s) 77, 160, 331, 523, 553, 1327
BstMWI GCNNNNNNNGC 4 cut(s) 66, 182, 762, 902
BstNI CCWGG 2 cut(s) 846, 1203
BstSCI CCNGG 3 cut(s) 556, 844, 1201
BstSFI CTRYAG 1 cut(s) 1237
BstSLI GKGCMC 1 cut(s) 852
BstUI CGCG 1 cut(s) 1140
BstV1I GCAGC 5 cut(s) 169, 188, 191, 670, 1289
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 3 cut(s) 77, 331, 1327
BstYI RGATCY 3 cut(s) 77, 331, 1327
BsuRI GGCC 5 cut(s) 361, 616, 784, 817, 850
BtsCI GGATG 4 cut(s) 83, 233, 793, 1219
BtsIMutI CAGTG 1 cut(s) 411
Cac8I GCNNGC 1 cut(s) 67
CaiI CAGNNNCTG 2 cut(s) 182, 185
Cfr13I GGNCC 3 cut(s) 848, 849, 1205
Csp6I GTAC 1 cut(s) 719
CviAII CATG 6 cut(s) 243, 452, 473, 808, 912, 978
CviQI GTAC 1 cut(s) 719
DdeI CTNAG 2 cut(s) 1057, 1149
DpnI GATC 6 cut(s) 79, 162, 333, 525, 555, 1329
DpnII GATC 6 cut(s) 77, 160, 331, 523, 553, 1327
DraI TTTAAA 1 cut(s) 865
EaeI YGGCCR 1 cut(s) 782
Eam1104I CTCTTC 3 cut(s) 304, 806, 1267
EarI CTCTTC 3 cut(s) 304, 806, 1267
Eco24I GRGCYC 2 cut(s) 741, 852
Eco47I GGWCC 1 cut(s) 1205
Eco57I CTGAAG 1 cut(s) 930
EcoO109I RGGNCCY 2 cut(s) 848, 849
EcoRI GAATTC 1 cut(s) 1003
EcoRII CCWGG 2 cut(s) 844, 1201
EcoT38I GRGCYC 2 cut(s) 741, 852
FaeI CATG 6 cut(s) 246, 455, 476, 811, 915, 981
FaqI GGGAC 4 cut(s) 239, 483, 1074, 1130
FatI CATG 6 cut(s) 242, 451, 472, 807, 911, 977
FbaI TGATCA 1 cut(s) 523
FblI GTMKAC 1 cut(s) 575
Fnu4HI GCNGC 5 cut(s) 177, 180, 183, 659, 1278
FokI GGATG 4 cut(s) 70, 220, 800, 1206
FriOI GRGCYC 2 cut(s) 741, 852
Fsp4HI GCNGC 5 cut(s) 177, 180, 183, 659, 1278
FspBI CTAG 7 cut(s) 39, 51, 75, 329, 833, 1124, 1313
GluI GCNGC 5 cut(s) 177, 180, 183, 659, 1278
GsaI CCCAGC 1 cut(s) 1095
HaeIII GGCC 5 cut(s) 361, 616, 784, 817, 850
HapII CCGG 2 cut(s) 558, 785
Hin1II CATG 6 cut(s) 246, 455, 476, 811, 915, 981
HincII GTYRAC 1 cut(s) 198
HindII GTYRAC 1 cut(s) 198
HindIII AAGCTT 2 cut(s) 1210, 1230
HinfI GANTC 3 cut(s) 596, 998, 1157
HpaII CCGG 2 cut(s) 558, 785
HphI GGTGA 6 cut(s) 210, 333, 613, 697, 1045, 1319
Hpy166II GTNNAC 3 cut(s) 198, 576, 1310
Hpy188I TCNGA 4 cut(s) 528, 732, 965, 997
Hpy188III TCNNGA 3 cut(s) 8, 521, 833
Hpy8I GTNNAC 3 cut(s) 198, 576, 1310
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4V TGCA 2 cut(s) 65, 1181
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 182, 762, 902
HpyF3I CTNAG 2 cut(s) 1057, 1149
Hsp92II CATG 6 cut(s) 246, 455, 476, 811, 915, 981
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 6 cut(s) 77, 160, 331, 523, 553, 1327
LguI GCTCTTC 1 cut(s) 1267
LmnI GCTCC 2 cut(s) 889, 940
Lsp1109I GCAGC 5 cut(s) 169, 188, 191, 670, 1289
MaeI CTAG 7 cut(s) 39, 51, 75, 329, 833, 1124, 1313
MaeIII GTNAC 1 cut(s) 509
MalI GATC 6 cut(s) 79, 162, 333, 525, 555, 1329
MboI GATC 6 cut(s) 77, 160, 331, 523, 553, 1327
MboII GAAGA 9 cut(s) 23, 72, 170, 198, 291, 803, 823, 1237, 1284
MfeI CAATTG 1 cut(s) 1297
MflI RGATCY 3 cut(s) 77, 331, 1327
MhlI GDGCHC 2 cut(s) 741, 852
MlyI GAGTC 2 cut(s) 605, 1007
MmeI TCCRAC 2 cut(s) 398, 624
MseI TTAA 3 cut(s) 368, 864, 1020
MslI CAYNNNNRTG 2 cut(s) 626, 778
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 2 cut(s) 558, 785
MspR9I CCNGG 3 cut(s) 558, 846, 1203
MunI CAATTG 1 cut(s) 1297
MvaI CCWGG 2 cut(s) 846, 1203
MvnI CGCG 1 cut(s) 1140
MwoI GCNNNNNNNGC 4 cut(s) 66, 182, 762, 902
NciI CCSGG 1 cut(s) 558
NdeII GATC 6 cut(s) 77, 160, 331, 523, 553, 1327
NlaIII CATG 6 cut(s) 246, 455, 476, 811, 915, 981
NlaIV GGNNCC 3 cut(s) 503, 850, 942
NmuCI GTSAC 1 cut(s) 509
OliI CACNNNNGTG 2 cut(s) 626, 778
PciSI GCTCTTC 1 cut(s) 1267
PfeI GAWTC 1 cut(s) 1157
PfoI TCCNGGA 1 cut(s) 556
PkrI GCNGC 5 cut(s) 178, 181, 184, 660, 1279
PleI GAGTC 2 cut(s) 604, 1006
PpsI GAGTC 2 cut(s) 604, 1006
PshBI ATTAAT 1 cut(s) 368
PsiI TTATAA 1 cut(s) 1345
Psp6I CCWGG 2 cut(s) 844, 1201
PspFI CCCAGC 1 cut(s) 1091
PspGI CCWGG 2 cut(s) 844, 1201
PspN4I GGNNCC 3 cut(s) 503, 850, 942
PspOMI GGGCCC 1 cut(s) 848
PspPI GGNCC 3 cut(s) 848, 849, 1205
PstNI CAGNNNCTG 2 cut(s) 182, 185
PsuI RGATCY 3 cut(s) 77, 331, 1327
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 1 cut(s) 720
RsaNI GTAC 1 cut(s) 719
RseI CAYNNNNRTG 2 cut(s) 626, 778
SapI GCTCTTC 1 cut(s) 1267
SaqAI TTAA 3 cut(s) 368, 864, 1020
SatI GCNGC 5 cut(s) 177, 180, 183, 659, 1278
Sau3AI GATC 6 cut(s) 77, 160, 331, 523, 553, 1327
Sau96I GGNCC 3 cut(s) 848, 849, 1205
SchI GAGTC 2 cut(s) 605, 1007
ScrFI CCNGG 3 cut(s) 558, 846, 1203
SduI GDGCHC 2 cut(s) 741, 852
SfcI CTRYAG 1 cut(s) 1237
SinI GGWCC 1 cut(s) 1205
SmiMI CAYNNNNRTG 2 cut(s) 626, 778
SmlI CTYRAG 2 cut(s) 6, 140
SmoI CTYRAG 2 cut(s) 6, 140
SpeI ACTAGT 1 cut(s) 38
SsiI CCGC 1 cut(s) 1138
SspMI CTAG 7 cut(s) 39, 51, 75, 329, 833, 1124, 1313
StyD4I CCNGG 3 cut(s) 556, 844, 1201
TaaI ACNGT 1 cut(s) 415
TaqI TCGA 1 cut(s) 1001
TaqII GACCGA 1 cut(s) 561
TatI WGTACW 1 cut(s) 718
TfiI GAWTC 1 cut(s) 1157
Tru1I TTAA 3 cut(s) 368, 864, 1020
Tru9I TTAA 3 cut(s) 368, 864, 1020
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 5 cut(s) 176, 179, 182, 658, 1277
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 7 cut(s) 72, 696, 804, 824, 900, 915, 966
TspRI CASTG 1 cut(s) 418
VpaK11BI GGWCC 1 cut(s) 1205
VspI ATTAAT 1 cut(s) 368
XapI RAATTY 5 cut(s) 462, 583, 866, 1003, 1192
XbaI TCTAGA 1 cut(s) 832
XmiI GTMKAC 1 cut(s) 575
XspI CTAG 7 cut(s) 39, 51, 75, 329, 833, 1124, 1313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.