RLG00000033324

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014693
Physical Location & Seq
Forward (+)
25730357 .. 25732620
2264 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033324

Sequence Viewer

Length: 1374 bp
ATGGGTAAAGAGGAAATAACCCAGAAGTGTACTAGTCCTACTGGTGGCTTTGGTAGATCCTCAAATTCCAGAAAGACTAATAAGCAAAAGAAAGTGCCACAGAGAGGTTTAGGTGTGGCTCAGCTTGAGAAGATCAGATTAGAAGAACAGCAAAAGAAAGCTGCTTCTGTTTTGACATCACCAACCCCACCTGCCTCTAATTCCTTACCATCACCAACCAAATCATCTCCATGTGTTCCTAATCCCGCTCCGATTAGACATTATCAACCACCCTCTTCAATCCCATTTCCATCACTGGATCTGCCCTCACCAAACTCGATTTTCAGGCCACCCAATGCCGATGTTGTTCGAATGCCCAACAGTATTAGTACTGTTCCATTGCCTAACAAGGGGACTCATGGGTTTGAGATTCCGGGACATGGAAATGTTGTTGTTCCCAACTTGTGGAATCCCAATTGTGAGTACAACATTGCTGATCTCAATAAGGAGAGTTCTTCAGGAATTTTGGATCCGGGATTCGCATTTCGGCCTAGTTTGAATAATTTGCCTACTTTCGATAACTCCAACGCGATTTGGGCTCTCCCCAATTTTCCACAAAGAACACACCAATTTCATCACCAACCACCACCACCACCACCATTGGTGAATGTCTCATCAGCTTCTTCATTATCGTCATCATCATCTGTACTAAACTTTCACACAGAGCCCCCTTCAAACCAAAGCTATCACAGCAACTATACTACACCAATGTGGTCAGAGGAAGAAAGGATGGTTGCCATGAAAAGGCCATATCCCTTTTCTCACGACAATGACGCTCCCTCAATCAACTTCAAATTCCCAATCTTTGCTCCTCCTCCTATGAGGACAGATGAAACAACTTCATGTGGGAACAGAGGAGGCACCTTCAATTTAGACCCTGACGGCAACACAATCTTCAGAGAAGGCTCTACCTGCTCAACTTCGATTTTGGAGTCCAATTCGAAGATTAGCATCAAAGAAAATGGGAGTGTTGATGGAGATTTTCTCACACTTGCCCCTCCTACAACTAATAGGACATGTCCGAGCCCAAAGTTTAATTCTCCTTCAGCTTATCTAGCATTCCATAACCCTGAAGTCCCCGATTTCGCCTCATTTCCTTATAAAGGAAGTTCAGAAGATACAATTTTACAGTCAGGACCAAGTGGTTCAACTCCACAACAGGCTTTCTACACCTTCATCCCACCAGCAAAATCACAAATTGTTAAACCAGCAGCAACCATAAACAATTGTGGGATGATGGATGACTCAAGGTACTGCTTTGCTAACAAACTCTTGCATGTGGATTGTAGAGGATCTGATATGGTTTGTCCTATCTTCCTTACTGGGGATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NOZZLE

Protein Analysis

458

Amino Acids

49.7

Weight (kDa)

7.59

Isoelectric Point (pI)

61.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g18750 FvH4_3g18750 FvH4_3g18750
malus_domestica MD03G1238800.v1.1 MD11G1258600.v1.1
prunus_persica Prupe.4G171200_v2.0.a1
pyrus_communis pycom11g22840
rosa_chinensis RchiOBHm_Chr5g0031561
rosa_laevigata RLG00000033324
rosa_multiflora Rmu_sc0014784.1_g000002
rosa_roxburghii Rroxscaffold_1G00048740
rosa_rugosa Rorug05G0127000
rosa_samantha Rh5AG220200 Rh5BG219600 Rh5CG243000 Rh5DG224700
rosa_wichuraiana Rw5G020080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1140
AarI CACCTGC 1 cut(s) 199
Acc36I ACCTGC 2 cut(s) 199, 959
AccB1I GGYRCC 1 cut(s) 899
AccB7I CCANNNNNTGG 1 cut(s) 444
AccBSI CCGCTC 1 cut(s) 248
AccII CGCG 1 cut(s) 569
AciI CCGC 1 cut(s) 246
AclWI GGATC 6 cut(s) 51, 306, 503, 516, 1339, 1361
AcsI RAATTY 3 cut(s) 64, 501, 833
AcuI CTGAAG 4 cut(s) 480, 919, 1068, 1131
AfaI GTAC 5 cut(s) 31, 370, 464, 687, 1292
AfiI CCNNNNNNNGG 8 cut(s) 44, 104, 389, 419, 444, 783, 1142, 1363
AflIII ACRYGT 1 cut(s) 1055
AgsI TTSAA 6 cut(s) 279, 538, 714, 832, 907, 1188
AhlI ACTAGT 1 cut(s) 32
AjuI GAANNNNNNNTTGG 2 cut(s) 1060, 1092
AleI CACNNNNGTG 1 cut(s) 748
AluBI AGCT 5 cut(s) 124, 161, 659, 723, 1088
AluI AGCT 5 cut(s) 124, 161, 659, 723, 1088
Alw26I GTCTC 1 cut(s) 655
AlwI GGATC 6 cut(s) 51, 306, 503, 516, 1339, 1361
AoxI GGCC 3 cut(s) 326, 527, 785
ApeKI GCWGC 2 cut(s) 161, 1250
ApoI RAATTY 3 cut(s) 64, 501, 833
AspS9I GGNCC 1 cut(s) 1175
AsuC2I CCSGG 2 cut(s) 414, 513
AsuHPI GGTGA 5 cut(s) 171, 204, 300, 608, 655
AsuII TTCGAA 2 cut(s) 349, 980
AvaII GGWCC 1 cut(s) 1175
BamHI GGATCC 2 cut(s) 508, 1366
BanI GGYRCC 1 cut(s) 899
BanII GRGCYC 3 cut(s) 580, 708, 1067
BbvI GCAGC 2 cut(s) 148, 1262
BccI CCATC 5 cut(s) 217, 298, 763, 1007, 1270
BceAI ACGGC 1 cut(s) 937
BcnI CCSGG 2 cut(s) 414, 513
BcoDI GTCTC 1 cut(s) 655
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 3 cut(s) 33, 531, 1094
BfuAI ACCTGC 2 cut(s) 199, 959
BisI GCNGC 2 cut(s) 162, 1251
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 2 cut(s) 163, 1252
BmcAI AGTACT 1 cut(s) 370
Bme1390I CCNGG 2 cut(s) 414, 513
Bme18I GGWCC 1 cut(s) 1175
BmgT120I GGNCC 1 cut(s) 1175
BmiI GGNNCC 3 cut(s) 510, 901, 1368
BmrFI CCNGG 2 cut(s) 414, 513
BmrI ACTGGG 1 cut(s) 1371
BmsI GCATC 1 cut(s) 999
BmuI ACTGGG 1 cut(s) 1371
BplI GAGNNNNNCTC 2 cut(s) 1008, 1040
Bpu1102I GCTNAGC 1 cut(s) 120
Bpu14I TTCGAA 2 cut(s) 349, 980
BpuEI CTTGAG 2 cut(s) 146, 1270
BpuMI CCSGG 2 cut(s) 414, 513
BsaBI GATNNNNATC 1 cut(s) 989
Bsc4I CCNNNNNNNGG 8 cut(s) 44, 104, 389, 419, 444, 783, 1142, 1363
Bse1I ACTGG 3 cut(s) 46, 300, 1366
Bse3DI GCAATG 2 cut(s) 377, 468
Bse8I GATNNNNATC 1 cut(s) 989
BseGI GGATG 4 cut(s) 774, 1215, 1278, 1285
BseJI GATNNNNATC 1 cut(s) 989
BseLI CCNNNNNNNGG 8 cut(s) 44, 104, 389, 419, 444, 783, 1142, 1363
BseMI GCAATG 2 cut(s) 377, 468
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 3 cut(s) 46, 300, 1366
BseRI GAGGAG 3 cut(s) 840, 843, 909
BseXI GCAGC 2 cut(s) 148, 1262
Bsh1236I CGCG 1 cut(s) 569
BshFI GGCC 3 cut(s) 328, 529, 787
BshNI GGYRCC 1 cut(s) 899
BsiSI CCGG 2 cut(s) 413, 512
BslFI GGGAC 3 cut(s) 406, 429, 1100
BslI CCNNNNNNNGG 8 cut(s) 44, 104, 389, 419, 444, 783, 1142, 1363
BsmAI GTCTC 1 cut(s) 655
BsmFI GGGAC 3 cut(s) 406, 429, 1100
BsmI GAATGC 2 cut(s) 357, 1097
BsnI GGCC 3 cut(s) 328, 529, 787
Bsp119I TTCGAA 2 cut(s) 349, 980
Bsp1286I GDGCHC 3 cut(s) 580, 708, 1067
Bsp143I GATC 7 cut(s) 56, 132, 298, 475, 508, 1331, 1366
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 1 cut(s) 246
BspANI GGCC 3 cut(s) 328, 529, 787
BspCNI CTCAG 1 cut(s) 133
BspFNI CGCG 1 cut(s) 569
BspLI GGNNCC 3 cut(s) 510, 901, 1368
BspMI ACCTGC 2 cut(s) 199, 959
BspPI GGATC 6 cut(s) 51, 306, 503, 516, 1339, 1361
BspT104I TTCGAA 2 cut(s) 349, 980
BspT107I GGYRCC 1 cut(s) 899
BsrBI CCGCTC 1 cut(s) 248
BsrDI GCAATG 2 cut(s) 377, 468
BsrI ACTGG 3 cut(s) 46, 300, 1366
BssMI GATC 7 cut(s) 56, 132, 298, 475, 508, 1331, 1366
Bst4CI ACNGT 3 cut(s) 362, 373, 1170
Bst6I CTCTTC 1 cut(s) 280
BstBI TTCGAA 2 cut(s) 349, 980
BstDEI CTNAG 1 cut(s) 120
BstENI CCTNNNNNAGG 1 cut(s) 1140
BstF5I GGATG 4 cut(s) 774, 1215, 1278, 1285
BstFNI CGCG 1 cut(s) 569
BstKTI GATC 7 cut(s) 59, 135, 301, 478, 511, 1334, 1369
BstMAI GTCTC 1 cut(s) 655
BstMBI GATC 7 cut(s) 56, 132, 298, 475, 508, 1331, 1366
BstMWI GCNNNNNNNGC 4 cut(s) 575, 729, 951, 1094
BstNSI RCATGY 2 cut(s) 1059, 1319
BstSCI CCNGG 2 cut(s) 412, 511
BstUI CGCG 1 cut(s) 569
BstV1I GCAGC 2 cut(s) 148, 1262
BstX2I RGATCY 5 cut(s) 56, 298, 508, 1331, 1366
BstYI RGATCY 5 cut(s) 56, 298, 508, 1331, 1366
BsuRI GGCC 3 cut(s) 328, 529, 787
BtsCI GGATG 4 cut(s) 774, 1215, 1278, 1285
BtsIMutI CAGTG 1 cut(s) 293
BveI ACCTGC 2 cut(s) 199, 959
Cfr13I GGNCC 1 cut(s) 1175
CseI GACGC 1 cut(s) 821
Csp6I GTAC 5 cut(s) 30, 369, 463, 686, 1291
CspCI CAANNNNNGTGG 2 cut(s) 621, 656
CviAII CATG 7 cut(s) 231, 398, 419, 778, 882, 1056, 1316
CviQI GTAC 5 cut(s) 30, 369, 463, 686, 1291
DdeI CTNAG 1 cut(s) 120
DpnI GATC 7 cut(s) 58, 134, 300, 477, 510, 1333, 1368
DpnII GATC 7 cut(s) 56, 132, 298, 475, 508, 1331, 1366
Eam1104I CTCTTC 1 cut(s) 280
EarI CTCTTC 1 cut(s) 280
Eco24I GRGCYC 3 cut(s) 580, 708, 1067
Eco47I GGWCC 1 cut(s) 1175
Eco57I CTGAAG 4 cut(s) 480, 919, 1068, 1131
EcoNI CCTNNNNNAGG 1 cut(s) 1140
EcoT38I GRGCYC 3 cut(s) 580, 708, 1067
FaeI CATG 7 cut(s) 234, 401, 422, 781, 885, 1059, 1319
FaqI GGGAC 3 cut(s) 406, 429, 1100
FatI CATG 7 cut(s) 230, 397, 418, 777, 881, 1055, 1315
FauI CCCGC 1 cut(s) 253
Fnu4HI GCNGC 2 cut(s) 162, 1251
FokI GGATG 4 cut(s) 781, 1202, 1285, 1292
FriOI GRGCYC 3 cut(s) 580, 708, 1067
Fsp4HI GCNGC 2 cut(s) 162, 1251
FspBI CTAG 3 cut(s) 33, 531, 1094
GluI GCNGC 2 cut(s) 162, 1251
HaeIII GGCC 3 cut(s) 328, 529, 787
HapII CCGG 2 cut(s) 413, 512
HgaI GACGC 1 cut(s) 821
Hin1II CATG 7 cut(s) 234, 401, 422, 781, 885, 1059, 1319
HinfI GANTC 6 cut(s) 394, 409, 448, 516, 971, 1283
HpaII CCGG 2 cut(s) 413, 512
HphI GGTGA 5 cut(s) 171, 204, 300, 608, 655
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 7 cut(s) 137, 252, 757, 938, 1062, 1153, 1336
Hpy188III TCNNGA 4 cut(s) 69, 498, 803, 1173
Hpy8I GTNNAC 1 cut(s) 30
HpyAV CCTTC 5 cut(s) 720, 913, 935, 1092, 1222
HpyCH4III ACNGT 3 cut(s) 362, 373, 1170
HpyCH4V TGCA 1 cut(s) 1315
HpyF10VI GCNNNNNNNGC 4 cut(s) 575, 729, 951, 1094
HpyF3I CTNAG 1 cut(s) 120
Hsp92II CATG 7 cut(s) 234, 401, 422, 781, 885, 1059, 1319
Kzo9I GATC 7 cut(s) 56, 132, 298, 475, 508, 1331, 1366
LmnI GCTCC 3 cut(s) 253, 820, 853
Lsp1109I GCAGC 2 cut(s) 148, 1262
LweI GCATC 1 cut(s) 999
MaeI CTAG 3 cut(s) 33, 531, 1094
MalI GATC 7 cut(s) 58, 134, 300, 477, 510, 1333, 1368
MbiI CCGCTC 1 cut(s) 248
MboI GATC 7 cut(s) 56, 132, 298, 475, 508, 1331, 1366
MfeI CAATTG 2 cut(s) 454, 1264
MflI RGATCY 5 cut(s) 56, 298, 508, 1331, 1366
MhlI GDGCHC 3 cut(s) 580, 708, 1067
MlyI GAGTC 3 cut(s) 388, 980, 1277
MmeI TCCRAC 1 cut(s) 588
MseI TTAA 2 cut(s) 1074, 1242
MslI CAYNNNNRTG 4 cut(s) 229, 423, 748, 807
MspI CCGG 2 cut(s) 413, 512
MspR9I CCNGG 2 cut(s) 414, 513
MunI CAATTG 2 cut(s) 454, 1264
Mva1269I GAATGC 2 cut(s) 357, 1097
MvnI CGCG 1 cut(s) 569
MwoI GCNNNNNNNGC 4 cut(s) 575, 729, 951, 1094
NciI CCSGG 2 cut(s) 414, 513
NdeII GATC 7 cut(s) 56, 132, 298, 475, 508, 1331, 1366
NlaIII CATG 7 cut(s) 234, 401, 422, 781, 885, 1059, 1319
NlaIV GGNNCC 3 cut(s) 510, 901, 1368
NspI RCATGY 2 cut(s) 1059, 1319
NspV TTCGAA 2 cut(s) 349, 980
OliI CACNNNNGTG 1 cut(s) 748
PaqCI CACCTGC 1 cut(s) 199
PciI ACATGT 1 cut(s) 1055
PctI GAATGC 2 cut(s) 357, 1097
PfeI GAWTC 3 cut(s) 409, 448, 516
PflMI CCANNNNNTGG 1 cut(s) 444
PfoI TCCNGGA 2 cut(s) 412, 511
PkrI GCNGC 2 cut(s) 163, 1252
PleI GAGTC 3 cut(s) 388, 979, 1277
PpsI GAGTC 3 cut(s) 388, 979, 1277
PscI ACATGT 1 cut(s) 1055
PsiI TTATAA 1 cut(s) 1140
PspN4I GGNNCC 3 cut(s) 510, 901, 1368
PspPI GGNCC 1 cut(s) 1175
PsuI RGATCY 5 cut(s) 56, 298, 508, 1331, 1366
RsaI GTAC 5 cut(s) 31, 370, 464, 687, 1292
RsaNI GTAC 5 cut(s) 30, 369, 463, 686, 1291
RseI CAYNNNNRTG 4 cut(s) 229, 423, 748, 807
SaqAI TTAA 2 cut(s) 1074, 1242
SatI GCNGC 2 cut(s) 162, 1251
Sau3AI GATC 7 cut(s) 56, 132, 298, 475, 508, 1331, 1366
Sau96I GGNCC 1 cut(s) 1175
ScaI AGTACT 1 cut(s) 370
SchI GAGTC 3 cut(s) 388, 980, 1277
ScrFI CCNGG 2 cut(s) 414, 513
SduI GDGCHC 3 cut(s) 580, 708, 1067
SfaNI GCATC 1 cut(s) 999
SfuI TTCGAA 2 cut(s) 349, 980
SinI GGWCC 1 cut(s) 1175
SmiMI CAYNNNNRTG 4 cut(s) 229, 423, 748, 807
SmlI CTYRAG 2 cut(s) 125, 1285
SmoI CTYRAG 2 cut(s) 125, 1285
SpeI ACTAGT 1 cut(s) 32
SsiI CCGC 1 cut(s) 246
SspMI CTAG 3 cut(s) 33, 531, 1094
StyD4I CCNGG 2 cut(s) 412, 511
TaaI ACNGT 3 cut(s) 362, 373, 1170
TaqI TCGA 5 cut(s) 317, 349, 555, 962, 980
TatI WGTACW 4 cut(s) 29, 368, 462, 685
TfiI GAWTC 3 cut(s) 409, 448, 516
Tru1I TTAA 2 cut(s) 1074, 1242
Tru9I TTAA 2 cut(s) 1074, 1242
TscAI CASTG 1 cut(s) 300
TseI GCWGC 2 cut(s) 161, 1250
TspDTI ATGAA 6 cut(s) 602, 654, 794, 870, 885, 1204
TspRI CASTG 1 cut(s) 300
Van91I CCANNNNNTGG 1 cut(s) 444
VpaK11BI GGWCC 1 cut(s) 1175
XagI CCTNNNNNAGG 1 cut(s) 1140
XapI RAATTY 3 cut(s) 64, 501, 833
XceI RCATGY 2 cut(s) 1059, 1319
XspI CTAG 3 cut(s) 33, 531, 1094
ZrmI AGTACT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.