Rw5G020080

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0014693
Physical Location & Seq
Forward (+)
26222262 .. 26224889
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020080.1

Sequence Viewer

Length: 1326 bp
ATGGGTAAAGAGGAAATAACCCAGAAGTGTACTAGTCCTACTGGTGGCTTTGGTAGATCCTCAAATTCCAAAAAGACTAATAAGCAAAAGAAAGTGCCACAGAGAGGTTTAGGTGTGGCTCAGCTTGAGAAGATCAGATTAGAAGAACAGCAAAAGAAAGCTGCTTCTGTTTTGACATCACCAACCCCACCTGCCTCTAATTCCTTACCATCACCAACCAAATCATCTCCATGTGTTCCTAATCCCGCTCCGATTAGACATTACCAACCACCCTCTTCAATCCCATTTCCATCTCTGCCATCACTGGATCTGCCCTCACCAAACTCGATTTTCAGGCCACCCAATGCCGATGTTGTTCGAAAGCCGAACAGTGTTAGTACTGTTCCATTGCCTAACAAGGGGACTCATGGGTTTGAGATTCCGGGACATGGAAATGTTGTTGTTCCCAACTTGTGGAATCCCAATTGTGAGTACAACAGTGCTGATCTCAATAAGGAGAGTTCATCAGGAATTTTGGATCCGGGATTCGCATTTCGGCCTAGTTTGAATAATTTGCCTACTTTCGATAACTCCAACGCGATTTGGGCTCTCCCCAATTTTCCACAAAGAACACACCAATTTCATCACCAACCACCACCACCTCCTCCTTCATTGGTGAATGTCTCATCAGCTACTTCATTATCGTCTTCATCATCTGTACTAAACTTTCACACAGAGCCCCCTTCAAACCAAAGCTATTACAGCAACTATACTACACCAATGTGGTCAGAGGAAGAAAGGATGGTTGGCATGAAAAGGCTATATCCCTTTTCTCACGACAATGACGCTCCCTCAATCAACTTCAAATTCCCAACCTTTGCTCCTCCTCCTATGAGGACAGATGAAACAACTTCATGTGGGAACAGAGGAGGCACCTCTAATTTAGACCCTGACGGCAACACAATCTTCAGAGAAGGCTCTACCTGCTCAACTAAAATTTCTGAGTCCAATTCGAAGATTAGCATCAAAGAAAATGGGAGTGTTAATGGAGATTTTCTCACACTTGCCCCTCCTACAACTAATAGGACATGTCCGAGCCCAAAGTTTAATTTTCCTCCAGCTTATCTAGCATTCCATAACCCTGAAGTCCCCGATTTTGCCTCATTTCCTTATAAAGGAAGTTCAGAAGATACAATTTTACAGTCAGGACCAAGTGGTTCAACTCCACAACAGGCTTTCTACACCTTCATCCCACCAGCAAAATCACAAATTGTTAAACCAGCAGCAACCATAAACAATTGTAATGGTGAAGTAGGAGAAACTGTTGATCTCAATTTGAAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NOZZLE

Protein Analysis

441

Amino Acids

47.76

Weight (kDa)

8.56

Isoelectric Point (pI)

57.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g18750 FvH4_3g18750 FvH4_3g18750
malus_domestica MD03G1238800.v1.1 MD11G1258600.v1.1
prunus_persica Prupe.4G171200_v2.0.a1
pyrus_communis pycom11g22840
rosa_chinensis RchiOBHm_Chr5g0031561
rosa_laevigata RLG00000033324
rosa_multiflora Rmu_sc0014784.1_g000002
rosa_roxburghii Rroxscaffold_1G00048740
rosa_rugosa Rorug05G0127000
rosa_samantha Rh5AG220200 Rh5BG219600 Rh5CG243000 Rh5DG224700
rosa_wichuraiana Rw5G020080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1152, 1324
AarI CACCTGC 1 cut(s) 199
Acc36I ACCTGC 2 cut(s) 199, 971
AccB1I GGYRCC 1 cut(s) 911
AccB7I CCANNNNNTGG 1 cut(s) 453
AccBSI CCGCTC 1 cut(s) 248
AccII CGCG 1 cut(s) 578
AciI CCGC 1 cut(s) 246
AclWI GGATC 4 cut(s) 51, 315, 512, 525
AcsI RAATTY 4 cut(s) 64, 510, 845, 975
AcuI CTGAAG 2 cut(s) 931, 1143
AfaI GTAC 4 cut(s) 31, 379, 473, 699
AfiI CCNNNNNNNGG 6 cut(s) 44, 104, 398, 428, 453, 1154
AflIII ACRYGT 1 cut(s) 1067
AgsI TTSAA 6 cut(s) 279, 547, 726, 844, 1200, 1318
AhlI ACTAGT 1 cut(s) 32
AjuI GAANNNNNNNTTGG 2 cut(s) 768, 800
AleI CACNNNNGTG 1 cut(s) 760
AluBI AGCT 5 cut(s) 124, 161, 671, 735, 1100
AluI AGCT 5 cut(s) 124, 161, 671, 735, 1100
Alw26I GTCTC 1 cut(s) 667
AlwI GGATC 4 cut(s) 51, 315, 512, 525
AoxI GGCC 2 cut(s) 335, 536
ApeKI GCWGC 2 cut(s) 161, 1262
ApoI RAATTY 4 cut(s) 64, 510, 845, 975
AspS9I GGNCC 1 cut(s) 1187
AsuC2I CCSGG 2 cut(s) 423, 522
AsuHPI GGTGA 6 cut(s) 171, 204, 309, 617, 667, 1298
AsuII TTCGAA 2 cut(s) 358, 992
AvaII GGWCC 1 cut(s) 1187
BamHI GGATCC 1 cut(s) 517
BanI GGYRCC 1 cut(s) 911
BanII GRGCYC 3 cut(s) 589, 720, 1079
BbsI GAAGAC 1 cut(s) 678
BbvI GCAGC 2 cut(s) 148, 1274
BccI CCATC 4 cut(s) 217, 298, 307, 775
BceAI ACGGC 1 cut(s) 949
BcnI CCSGG 2 cut(s) 423, 522
BcoDI GTCTC 1 cut(s) 667
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 3 cut(s) 33, 540, 1106
BfuAI ACCTGC 2 cut(s) 199, 971
BisI GCNGC 2 cut(s) 162, 1263
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 2 cut(s) 163, 1264
BmcAI AGTACT 1 cut(s) 379
Bme1390I CCNGG 2 cut(s) 423, 522
Bme18I GGWCC 1 cut(s) 1187
BmgT120I GGNCC 1 cut(s) 1187
BmiI GGNNCC 2 cut(s) 519, 913
BmrFI CCNGG 2 cut(s) 423, 522
BmsI GCATC 1 cut(s) 1011
BpiI GAAGAC 1 cut(s) 678
BplI GAGNNNNNCTC 2 cut(s) 1020, 1052
BpmI CTGGAG 1 cut(s) 1080
Bpu1102I GCTNAGC 1 cut(s) 120
Bpu14I TTCGAA 2 cut(s) 358, 992
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 2 cut(s) 423, 522
BsaBI GATNNNNATC 1 cut(s) 1001
BsaXI ACNNNNNCTCC 4 cut(s) 844, 874, 1287, 1317
Bsc4I CCNNNNNNNGG 6 cut(s) 44, 104, 398, 428, 453, 1154
Bse1I ACTGG 2 cut(s) 46, 309
Bse3DI GCAATG 1 cut(s) 386
Bse8I GATNNNNATC 1 cut(s) 1001
BseGI GGATG 2 cut(s) 786, 1227
BseJI GATNNNNATC 1 cut(s) 1001
BseLI CCNNNNNNNGG 6 cut(s) 44, 104, 398, 428, 453, 1154
BseMI GCAATG 1 cut(s) 386
BseMII CTCAG 2 cut(s) 134, 972
BseNI ACTGG 2 cut(s) 46, 309
BseRI GAGGAG 4 cut(s) 633, 852, 855, 921
BseXI GCAGC 2 cut(s) 148, 1274
Bsh1236I CGCG 1 cut(s) 578
BshFI GGCC 2 cut(s) 337, 538
BshNI GGYRCC 1 cut(s) 911
BsiSI CCGG 2 cut(s) 422, 521
BslFI GGGAC 3 cut(s) 415, 438, 1112
BslI CCNNNNNNNGG 6 cut(s) 44, 104, 398, 428, 453, 1154
BsmAI GTCTC 1 cut(s) 667
BsmFI GGGAC 3 cut(s) 415, 438, 1112
BsmI GAATGC 1 cut(s) 1109
BsnI GGCC 2 cut(s) 337, 538
Bsp119I TTCGAA 2 cut(s) 358, 992
Bsp1286I GDGCHC 3 cut(s) 589, 720, 1079
Bsp143I GATC 6 cut(s) 56, 132, 307, 484, 517, 1306
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 1 cut(s) 246
BspANI GGCC 2 cut(s) 337, 538
BspCNI CTCAG 2 cut(s) 133, 973
BspFNI CGCG 1 cut(s) 578
BspLI GGNNCC 2 cut(s) 519, 913
BspMI ACCTGC 2 cut(s) 199, 971
BspPI GGATC 4 cut(s) 51, 315, 512, 525
BspT104I TTCGAA 2 cut(s) 358, 992
BspT107I GGYRCC 1 cut(s) 911
BsrBI CCGCTC 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 386
BsrI ACTGG 2 cut(s) 46, 309
BssMI GATC 6 cut(s) 56, 132, 307, 484, 517, 1306
Bst4CI ACNGT 5 cut(s) 371, 382, 479, 1182, 1303
Bst6I CTCTTC 1 cut(s) 280
BstBI TTCGAA 2 cut(s) 358, 992
BstDEI CTNAG 2 cut(s) 120, 981
BstENI CCTNNNNNAGG 1 cut(s) 1152
BstF5I GGATG 2 cut(s) 786, 1227
BstFNI CGCG 1 cut(s) 578
BstKTI GATC 6 cut(s) 59, 135, 310, 487, 520, 1309
BstMAI GTCTC 1 cut(s) 667
BstMBI GATC 6 cut(s) 56, 132, 307, 484, 517, 1306
BstMWI GCNNNNNNNGC 4 cut(s) 584, 741, 963, 1106
BstNSI RCATGY 1 cut(s) 1071
BstSCI CCNGG 2 cut(s) 421, 520
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 2 cut(s) 148, 1274
BstV2I GAAGAC 1 cut(s) 678
BstX2I RGATCY 3 cut(s) 56, 307, 517
BstYI RGATCY 3 cut(s) 56, 307, 517
BsuRI GGCC 2 cut(s) 337, 538
BtsCI GGATG 2 cut(s) 786, 1227
BtsIMutI CAGTG 3 cut(s) 302, 376, 484
BveI ACCTGC 2 cut(s) 199, 971
Cfr13I GGNCC 1 cut(s) 1187
CseI GACGC 1 cut(s) 833
Csp6I GTAC 4 cut(s) 30, 378, 472, 698
CviAII CATG 6 cut(s) 231, 407, 428, 790, 894, 1068
CviQI GTAC 4 cut(s) 30, 378, 472, 698
DdeI CTNAG 2 cut(s) 120, 981
DpnI GATC 6 cut(s) 58, 134, 309, 486, 519, 1308
DpnII GATC 6 cut(s) 56, 132, 307, 484, 517, 1306
Eam1104I CTCTTC 1 cut(s) 280
EarI CTCTTC 1 cut(s) 280
Eco24I GRGCYC 3 cut(s) 589, 720, 1079
Eco47I GGWCC 1 cut(s) 1187
Eco57I CTGAAG 2 cut(s) 931, 1143
EcoNI CCTNNNNNAGG 1 cut(s) 1152
EcoT38I GRGCYC 3 cut(s) 589, 720, 1079
FaeI CATG 6 cut(s) 234, 410, 431, 793, 897, 1071
FaqI GGGAC 3 cut(s) 415, 438, 1112
FatI CATG 6 cut(s) 230, 406, 427, 789, 893, 1067
FauI CCCGC 1 cut(s) 253
Fnu4HI GCNGC 2 cut(s) 162, 1263
FokI GGATG 2 cut(s) 793, 1214
FriOI GRGCYC 3 cut(s) 589, 720, 1079
Fsp4HI GCNGC 2 cut(s) 162, 1263
FspBI CTAG 3 cut(s) 33, 540, 1106
GluI GCNGC 2 cut(s) 162, 1263
GsuI CTGGAG 1 cut(s) 1080
HaeIII GGCC 2 cut(s) 337, 538
HapII CCGG 2 cut(s) 422, 521
HgaI GACGC 1 cut(s) 833
Hin1II CATG 6 cut(s) 234, 410, 431, 793, 897, 1071
HinfI GANTC 5 cut(s) 403, 418, 457, 525, 983
HpaII CCGG 2 cut(s) 422, 521
HphI GGTGA 6 cut(s) 171, 204, 309, 617, 667, 1298
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 7 cut(s) 137, 252, 769, 950, 982, 1074, 1165
Hpy188III TCNNGA 3 cut(s) 507, 815, 1185
Hpy8I GTNNAC 1 cut(s) 30
HpyAV CCTTC 4 cut(s) 657, 732, 947, 1234
HpyCH4III ACNGT 5 cut(s) 371, 382, 479, 1182, 1303
HpyF10VI GCNNNNNNNGC 4 cut(s) 584, 741, 963, 1106
HpyF3I CTNAG 2 cut(s) 120, 981
Hsp92II CATG 6 cut(s) 234, 410, 431, 793, 897, 1071
Kzo9I GATC 6 cut(s) 56, 132, 307, 484, 517, 1306
LmnI GCTCC 3 cut(s) 253, 832, 865
Lsp1109I GCAGC 2 cut(s) 148, 1274
LweI GCATC 1 cut(s) 1011
MaeI CTAG 3 cut(s) 33, 540, 1106
MalI GATC 6 cut(s) 58, 134, 309, 486, 519, 1308
MbiI CCGCTC 1 cut(s) 248
MboI GATC 6 cut(s) 56, 132, 307, 484, 517, 1306
MboII GAAGA 8 cut(s) 142, 155, 267, 678, 785, 937, 1006, 1178
MfeI CAATTG 2 cut(s) 463, 1276
MflI RGATCY 3 cut(s) 56, 307, 517
MhlI GDGCHC 3 cut(s) 589, 720, 1079
MlyI GAGTC 2 cut(s) 397, 992
MmeI TCCRAC 1 cut(s) 597
MseI TTAA 3 cut(s) 1023, 1086, 1254
MslI CAYNNNNRTG 4 cut(s) 229, 432, 760, 819
MspI CCGG 2 cut(s) 422, 521
MspR9I CCNGG 2 cut(s) 423, 522
MunI CAATTG 2 cut(s) 463, 1276
Mva1269I GAATGC 1 cut(s) 1109
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 4 cut(s) 584, 741, 963, 1106
NciI CCSGG 2 cut(s) 423, 522
NdeII GATC 6 cut(s) 56, 132, 307, 484, 517, 1306
NlaIII CATG 6 cut(s) 234, 410, 431, 793, 897, 1071
NlaIV GGNNCC 2 cut(s) 519, 913
NspI RCATGY 1 cut(s) 1071
NspV TTCGAA 2 cut(s) 358, 992
OliI CACNNNNGTG 1 cut(s) 760
PaqCI CACCTGC 1 cut(s) 199
PciI ACATGT 1 cut(s) 1067
PctI GAATGC 1 cut(s) 1109
PfeI GAWTC 3 cut(s) 418, 457, 525
PflMI CCANNNNNTGG 1 cut(s) 453
PfoI TCCNGGA 2 cut(s) 421, 520
PkrI GCNGC 2 cut(s) 163, 1264
PleI GAGTC 2 cut(s) 397, 991
PpsI GAGTC 2 cut(s) 397, 991
PscI ACATGT 1 cut(s) 1067
PsiI TTATAA 2 cut(s) 1152, 1324
PspN4I GGNNCC 2 cut(s) 519, 913
PspPI GGNCC 1 cut(s) 1187
PsuI RGATCY 3 cut(s) 56, 307, 517
RsaI GTAC 4 cut(s) 31, 379, 473, 699
RsaNI GTAC 4 cut(s) 30, 378, 472, 698
RseI CAYNNNNRTG 4 cut(s) 229, 432, 760, 819
SaqAI TTAA 3 cut(s) 1023, 1086, 1254
SatI GCNGC 2 cut(s) 162, 1263
Sau3AI GATC 6 cut(s) 56, 132, 307, 484, 517, 1306
Sau96I GGNCC 1 cut(s) 1187
ScaI AGTACT 1 cut(s) 379
SchI GAGTC 2 cut(s) 397, 992
ScrFI CCNGG 2 cut(s) 423, 522
SduI GDGCHC 3 cut(s) 589, 720, 1079
SfaNI GCATC 1 cut(s) 1011
SfuI TTCGAA 2 cut(s) 358, 992
SinI GGWCC 1 cut(s) 1187
SmiMI CAYNNNNRTG 4 cut(s) 229, 432, 760, 819
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SpeI ACTAGT 1 cut(s) 32
SsiI CCGC 1 cut(s) 246
SspMI CTAG 3 cut(s) 33, 540, 1106
StyD4I CCNGG 2 cut(s) 421, 520
TaaI ACNGT 5 cut(s) 371, 382, 479, 1182, 1303
TaqI TCGA 4 cut(s) 326, 358, 564, 992
TatI WGTACW 4 cut(s) 29, 377, 471, 697
TfiI GAWTC 3 cut(s) 418, 457, 525
Tru1I TTAA 3 cut(s) 1023, 1086, 1254
Tru9I TTAA 3 cut(s) 1023, 1086, 1254
TscAI CASTG 3 cut(s) 309, 376, 484
TseI GCWGC 2 cut(s) 161, 1262
TspDTI ATGAA 9 cut(s) 492, 611, 639, 666, 678, 806, 882, 897, 1216
TspRI CASTG 3 cut(s) 309, 376, 484
Van91I CCANNNNNTGG 1 cut(s) 453
VpaK11BI GGWCC 1 cut(s) 1187
XagI CCTNNNNNAGG 1 cut(s) 1152
XapI RAATTY 4 cut(s) 64, 510, 845, 975
XceI RCATGY 1 cut(s) 1071
XspI CTAG 3 cut(s) 33, 540, 1106
ZrmI AGTACT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.