Rh5DG224700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014693
Physical Location & Seq
Forward (+)
25294719 .. 25297893
3175 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG224700.1

Sequence Viewer

Length: 1191 bp
ATGGGTAAAGAGGAAATAACCCAGAAGTGTACTAGTCCTACTGGTGGCTTTGGTAGATCCTCAAATTCCAAAAAGTCTAATAAGCAAAAGAAAGTGCCACAGAGAGGTTTAGGTGTGGCTCAGCTTGAGAAGATCAGATTAGAAGAACAGCAAAAGAAAGCTGCTTCTGTTTTGACATCACCAACCCCACCTGCCTCTAATTCCTTACCATCACCAACCAAATCATCTCCATGTGTTCCTAATTCCGCTCCGATTAGACATTACCAACCACCCTCTTCAATCCCATTTCAATCTCTGCCATCACTGGATCTGCCCTCACCAAACTCGATTTTCAGGCCACCCAATGCCGATGTTGTTCGAAAGCCGAACAGTATTAGTACTGTTCCATTGCCTAACAAGGAGACTCATGGGTTTGAGATTCCGGGACATGGAAATGTTGTCGTTCCCAACTTGTGGAATCCCAATTGTGAGTACAACAGTGCTGATCTCAATAAGGAGAGTTCATCACGAATTTTGGATCCGGGATTCGCATTTCGGCCTAGTTTGAATAATTTGCCTACTTTCGATAACTCCAACGCGATTTGGGCTCTCCCCAATTTTCCACAAAGAAGACACCAATTTCATCACCAACCACCACCACCTCCTCCTTCATTGGTGAATGTCTCATCAGCTACTTCATTATCGTCATCATCATCTGTACTGAACTTTCACACAGAGCCCCCTTCAAACCAAAGCTATCACAGCATCTATACTACACCAATGTGGTCAGAGGAAGAGAGGATGGTTGGCATGAAAAGGCTATATCCCTTTTCTCACGACAATGACGCTCCCTCAATCAACTTCAAATTCCCAACCTTTGCTCCTCCTCCTATGAGGACAGATGAAACAACTTCATGTGGGAACAGAGGAGGCACCTCTAATTTAGACCCTGACGACAACACAATCTTCAGGTTTGCAAATCTTCATCTTTGTGATCCATTTAAATCCCCCAGTACATGCTTCCTTACATGGTTTTCATTTGCACCATTTTCAGAGAAGGCTCTACCTGCTCAACTAAAATTTCGGAGTCCAATTCGAAGATTAGCATCAAAGAAAATGGGAATGTTAATGGAGATTTTCTCACACTTGCCCCTCCTACAACTAATAGGACATGTCCGAGCCCAAAGTTTAATTTTCCTCCAGCTTATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NOZZLE

Protein Analysis

396

Amino Acids

43.9

Weight (kDa)

9.51

Isoelectric Point (pI)

68.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014693)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g18750 FvH4_3g18750 FvH4_3g18750
malus_domestica MD03G1238800.v1.1 MD11G1258600.v1.1
prunus_persica Prupe.4G171200_v2.0.a1
pyrus_communis pycom11g22840
rosa_chinensis RchiOBHm_Chr5g0031561
rosa_laevigata RLG00000033324
rosa_multiflora Rmu_sc0014784.1_g000002
rosa_roxburghii Rroxscaffold_1G00048740
rosa_rugosa Rorug05G0127000
rosa_samantha Rh5AG220200 Rh5BG219600 Rh5CG243000 Rh5DG224700
rosa_wichuraiana Rw5G020080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 199
Acc36I ACCTGC 2 cut(s) 199, 1054
AccB1I GGYRCC 1 cut(s) 911
AccB7I CCANNNNNTGG 1 cut(s) 453
AccBSI CCGCTC 1 cut(s) 248
AccII CGCG 1 cut(s) 578
AciI CCGC 1 cut(s) 246
AclWI GGATC 5 cut(s) 51, 315, 512, 525, 968
AcsI RAATTY 4 cut(s) 64, 510, 845, 1058
AcuI CTGAAG 1 cut(s) 931
AfaI GTAC 5 cut(s) 31, 379, 473, 699, 994
AfiI CCNNNNNNNGG 4 cut(s) 44, 104, 428, 453
AflIII ACRYGT 1 cut(s) 1150
AgsI TTSAA 5 cut(s) 279, 290, 547, 726, 844
AhlI ACTAGT 1 cut(s) 32
AleI CACNNNNGTG 1 cut(s) 760
AluBI AGCT 5 cut(s) 124, 161, 671, 735, 1183
AluI AGCT 5 cut(s) 124, 161, 671, 735, 1183
Alw26I GTCTC 2 cut(s) 395, 667
AlwI GGATC 5 cut(s) 51, 315, 512, 525, 968
AoxI GGCC 2 cut(s) 335, 536
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 4 cut(s) 64, 510, 845, 1058
AsuC2I CCSGG 2 cut(s) 423, 522
AsuHPI GGTGA 5 cut(s) 171, 204, 309, 617, 667
AsuII TTCGAA 2 cut(s) 358, 1075
BamHI GGATCC 1 cut(s) 517
BanI GGYRCC 1 cut(s) 911
BanII GRGCYC 3 cut(s) 589, 720, 1162
BbsI GAAGAC 1 cut(s) 616
BbvI GCAGC 1 cut(s) 148
BccI CCATC 3 cut(s) 217, 307, 775
BcnI CCSGG 2 cut(s) 423, 522
BcoDI GTCTC 2 cut(s) 395, 667
BcuI ACTAGT 1 cut(s) 32
BfaI CTAG 3 cut(s) 33, 540, 1189
BfuAI ACCTGC 2 cut(s) 199, 1054
BisI GCNGC 1 cut(s) 162
BlpI GCTNAGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 163
BmcAI AGTACT 1 cut(s) 379
Bme1390I CCNGG 2 cut(s) 423, 522
BmiI GGNNCC 2 cut(s) 519, 913
BmrFI CCNGG 2 cut(s) 423, 522
BmrI ACTGGG 1 cut(s) 984
BmsI GCATC 2 cut(s) 753, 1094
BmuI ACTGGG 1 cut(s) 984
BpiI GAAGAC 1 cut(s) 616
BplI GAGNNNNNCTC 2 cut(s) 1103, 1135
BpmI CTGGAG 1 cut(s) 1163
Bpu1102I GCTNAGC 1 cut(s) 120
Bpu14I TTCGAA 2 cut(s) 358, 1075
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 2 cut(s) 423, 522
BsaBI GATNNNNATC 1 cut(s) 1084
BsaXI ACNNNNNCTCC 2 cut(s) 844, 874
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 104, 428, 453
Bse1I ACTGG 3 cut(s) 46, 309, 990
Bse3DI GCAATG 1 cut(s) 386
Bse8I GATNNNNATC 1 cut(s) 1084
BseGI GGATG 1 cut(s) 786
BseJI GATNNNNATC 1 cut(s) 1084
BseLI CCNNNNNNNGG 4 cut(s) 44, 104, 428, 453
BseMI GCAATG 1 cut(s) 386
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 3 cut(s) 46, 309, 990
BseRI GAGGAG 4 cut(s) 633, 852, 855, 921
BseXI GCAGC 1 cut(s) 148
Bsh1236I CGCG 1 cut(s) 578
BshFI GGCC 2 cut(s) 337, 538
BshNI GGYRCC 1 cut(s) 911
BsiSI CCGG 2 cut(s) 422, 521
BslFI GGGAC 1 cut(s) 438
BslI CCNNNNNNNGG 4 cut(s) 44, 104, 428, 453
BsmAI GTCTC 2 cut(s) 395, 667
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 2 cut(s) 337, 538
Bsp119I TTCGAA 2 cut(s) 358, 1075
Bsp1286I GDGCHC 3 cut(s) 589, 720, 1162
Bsp143I GATC 6 cut(s) 56, 132, 307, 484, 517, 973
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 1 cut(s) 246
BspANI GGCC 2 cut(s) 337, 538
BspCNI CTCAG 1 cut(s) 133
BspFNI CGCG 1 cut(s) 578
BspLI GGNNCC 2 cut(s) 519, 913
BspMI ACCTGC 2 cut(s) 199, 1054
BspPI GGATC 5 cut(s) 51, 315, 512, 525, 968
BspT104I TTCGAA 2 cut(s) 358, 1075
BspT107I GGYRCC 1 cut(s) 911
BsrBI CCGCTC 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 386
BsrI ACTGG 3 cut(s) 46, 309, 990
BssMI GATC 6 cut(s) 56, 132, 307, 484, 517, 973
Bst4CI ACNGT 3 cut(s) 371, 382, 479
Bst6I CTCTTC 2 cut(s) 280, 768
BstBI TTCGAA 2 cut(s) 358, 1075
BstDEI CTNAG 1 cut(s) 120
BstF5I GGATG 1 cut(s) 786
BstFNI CGCG 1 cut(s) 578
BstKTI GATC 6 cut(s) 59, 135, 310, 487, 520, 976
BstMAI GTCTC 2 cut(s) 395, 667
BstMBI GATC 6 cut(s) 56, 132, 307, 484, 517, 973
BstMWI GCNNNNNNNGC 3 cut(s) 584, 741, 1046
BstNSI RCATGY 2 cut(s) 999, 1154
BstSCI CCNGG 2 cut(s) 421, 520
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 1 cut(s) 148
BstV2I GAAGAC 1 cut(s) 616
BstX2I RGATCY 3 cut(s) 56, 307, 517
BstYI RGATCY 3 cut(s) 56, 307, 517
BsuRI GGCC 2 cut(s) 337, 538
BtsCI GGATG 1 cut(s) 786
BtsIMutI CAGTG 2 cut(s) 302, 484
BveI ACCTGC 2 cut(s) 199, 1054
CseI GACGC 1 cut(s) 833
Csp6I GTAC 5 cut(s) 30, 378, 472, 698, 993
CviAII CATG 8 cut(s) 231, 407, 428, 790, 894, 996, 1008, 1151
CviQI GTAC 5 cut(s) 30, 378, 472, 698, 993
DdeI CTNAG 1 cut(s) 120
DpnI GATC 6 cut(s) 58, 134, 309, 486, 519, 975
DpnII GATC 6 cut(s) 56, 132, 307, 484, 517, 973
DraI TTTAAA 1 cut(s) 982
Eam1104I CTCTTC 2 cut(s) 280, 768
EarI CTCTTC 2 cut(s) 280, 768
Eco24I GRGCYC 3 cut(s) 589, 720, 1162
Eco57I CTGAAG 1 cut(s) 931
EcoT38I GRGCYC 3 cut(s) 589, 720, 1162
FaeI CATG 8 cut(s) 234, 410, 431, 793, 897, 999, 1011, 1154
FaqI GGGAC 1 cut(s) 438
FatI CATG 8 cut(s) 230, 406, 427, 789, 893, 995, 1007, 1150
Fnu4HI GCNGC 1 cut(s) 162
FokI GGATG 1 cut(s) 793
FriOI GRGCYC 3 cut(s) 589, 720, 1162
Fsp4HI GCNGC 1 cut(s) 162
FspBI CTAG 3 cut(s) 33, 540, 1189
GluI GCNGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 1163
HaeIII GGCC 2 cut(s) 337, 538
HapII CCGG 2 cut(s) 422, 521
HgaI GACGC 1 cut(s) 833
Hin1II CATG 8 cut(s) 234, 410, 431, 793, 897, 999, 1011, 1154
HinfI GANTC 5 cut(s) 403, 418, 457, 525, 1066
HpaII CCGG 2 cut(s) 422, 521
HphI GGTGA 5 cut(s) 171, 204, 309, 617, 667
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 6 cut(s) 137, 252, 769, 1033, 1065, 1157
Hpy188III TCNNGA 2 cut(s) 507, 815
Hpy8I GTNNAC 1 cut(s) 30
HpyAV CCTTC 3 cut(s) 657, 732, 1030
HpyCH4III ACNGT 3 cut(s) 371, 382, 479
HpyCH4V TGCA 2 cut(s) 956, 1022
HpyF10VI GCNNNNNNNGC 3 cut(s) 584, 741, 1046
HpyF3I CTNAG 1 cut(s) 120
Hsp92II CATG 8 cut(s) 234, 410, 431, 793, 897, 999, 1011, 1154
Kzo9I GATC 6 cut(s) 56, 132, 307, 484, 517, 973
LmnI GCTCC 3 cut(s) 253, 832, 865
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 2 cut(s) 753, 1094
MaeI CTAG 3 cut(s) 33, 540, 1189
MalI GATC 6 cut(s) 58, 134, 309, 486, 519, 975
MbiI CCGCTC 1 cut(s) 248
MboI GATC 6 cut(s) 56, 132, 307, 484, 517, 973
MboII GAAGA 8 cut(s) 142, 155, 267, 621, 785, 937, 953, 1089
MfeI CAATTG 1 cut(s) 463
MflI RGATCY 3 cut(s) 56, 307, 517
MhlI GDGCHC 3 cut(s) 589, 720, 1162
MlyI GAGTC 2 cut(s) 397, 1075
MmeI TCCRAC 1 cut(s) 597
MseI TTAA 3 cut(s) 981, 1106, 1169
MslI CAYNNNNRTG 5 cut(s) 229, 432, 760, 819, 969
MspI CCGG 2 cut(s) 422, 521
MspR9I CCNGG 2 cut(s) 423, 522
MunI CAATTG 1 cut(s) 463
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 3 cut(s) 584, 741, 1046
NciI CCSGG 2 cut(s) 423, 522
NdeII GATC 6 cut(s) 56, 132, 307, 484, 517, 973
NlaIII CATG 8 cut(s) 234, 410, 431, 793, 897, 999, 1011, 1154
NlaIV GGNNCC 2 cut(s) 519, 913
NspI RCATGY 2 cut(s) 999, 1154
NspV TTCGAA 2 cut(s) 358, 1075
OliI CACNNNNGTG 1 cut(s) 760
PaqCI CACCTGC 1 cut(s) 199
PciI ACATGT 1 cut(s) 1150
PfeI GAWTC 3 cut(s) 418, 457, 525
PflMI CCANNNNNTGG 1 cut(s) 453
PfoI TCCNGGA 2 cut(s) 421, 520
PkrI GCNGC 1 cut(s) 163
PleI GAGTC 2 cut(s) 397, 1074
PpsI GAGTC 2 cut(s) 397, 1074
PscI ACATGT 1 cut(s) 1150
PspN4I GGNNCC 2 cut(s) 519, 913
PsuI RGATCY 3 cut(s) 56, 307, 517
RsaI GTAC 5 cut(s) 31, 379, 473, 699, 994
RsaNI GTAC 5 cut(s) 30, 378, 472, 698, 993
RseI CAYNNNNRTG 5 cut(s) 229, 432, 760, 819, 969
SaqAI TTAA 3 cut(s) 981, 1106, 1169
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 6 cut(s) 56, 132, 307, 484, 517, 973
ScaI AGTACT 1 cut(s) 379
SchI GAGTC 2 cut(s) 397, 1075
ScrFI CCNGG 2 cut(s) 423, 522
SduI GDGCHC 3 cut(s) 589, 720, 1162
SfaNI GCATC 2 cut(s) 753, 1094
SfuI TTCGAA 2 cut(s) 358, 1075
SmiI ATTTAAAT 1 cut(s) 982
SmiMI CAYNNNNRTG 5 cut(s) 229, 432, 760, 819, 969
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SpeI ACTAGT 1 cut(s) 32
SsiI CCGC 1 cut(s) 246
SspMI CTAG 3 cut(s) 33, 540, 1189
StyD4I CCNGG 2 cut(s) 421, 520
SwaI ATTTAAAT 1 cut(s) 982
TaaI ACNGT 3 cut(s) 371, 382, 479
TaqI TCGA 4 cut(s) 326, 358, 564, 1075
TatI WGTACW 5 cut(s) 29, 377, 471, 697, 992
TfiI GAWTC 3 cut(s) 418, 457, 525
Tru1I TTAA 3 cut(s) 981, 1106, 1169
Tru9I TTAA 3 cut(s) 981, 1106, 1169
TscAI CASTG 2 cut(s) 309, 484
TseI GCWGC 1 cut(s) 161
TspDTI ATGAA 9 cut(s) 492, 611, 639, 666, 806, 882, 897, 953, 1005
TspRI CASTG 2 cut(s) 309, 484
Van91I CCANNNNNTGG 1 cut(s) 453
XapI RAATTY 4 cut(s) 64, 510, 845, 1058
XceI RCATGY 2 cut(s) 999, 1154
XspI CTAG 3 cut(s) 33, 540, 1189
ZrmI AGTACT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.