MD03G1241400.v1.1

Belongs to the glycosyltransferase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009248
Physical Location & Seq
Forward (+)
32629817 .. 32633320
3504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1241400.v1.1.491

Sequence Viewer

Length: 711 bp
ATGCTAGAGTGGCTTGAAAAATGGGGTTGCAGCTTGGAAGAAGCCACTGGTTTGGCTTTCAGGTTTGTGATTGGTAAAACCAAGGATAAAGCAAAGATGGCGGAACTCGCCAAAGAGGTGGCTAAATTTGATGATTTCCTGCTATTGGATATTGAAGAGGAGTACAGTAAGCTCCCCTACAAAACGTTGGCTTTCTTCAAAGCTGCCTATGCACTTTATGATGCTGACTTCTATGTTAAAGCTGATGACGACATATACTTAAGGCCAGATCGCCTTTCACTGCTCTTGGCAAAAGAGCGTTCTCACTCTCAGACATACCTTGGGTGCATGAAAAAGGGCCCAGTCTTCACAGACCCGAAGCTTAAGTGGTATGAGCCACTTTCCTATTTGCTTGGAAGCGAGTACTTTCTCCATGCCTATGGTCCAATATATGCTCTTTCTGCTGATGTTGTTGCAAGTTTGGTTGCTCTTAGAAACAACAGTTTTCGAATGTTTAGCAATGAGGATGTCACAATTGGTGCATGGATGCTTGCAATGAATGTTAACCATGAGAACAATAAAGCATTGTGCTCACAGGAGTGTACACCATCATCTATCGCTGTGTGGGATATTCCGAAGTGCTCTGGGCTCTGCAATCCAGAAACCAAATTGCTAGAACTCCACCAGATGGACAGCTGCTCAAAAACTCCGACGGTGGAATCTGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Galactosyl_T

Protein Analysis

237

Amino Acids

26.81

Weight (kDa)

5.05

Isoelectric Point (pI)

37.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Galactosyl_T PF01762 9 - 186 2.6e-44 Galactosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 667
AciI CCGC 1 cut(s) 101
AclI AACGTT 1 cut(s) 185
AcsI RAATTY 1 cut(s) 125
AfaI GTAC 3 cut(s) 164, 404, 583
AfiI CCNNNNNNNGG 2 cut(s) 145, 667
AflII CTTAAG 2 cut(s) 259, 362
AgsI TTSAA 3 cut(s) 17, 155, 199
AleI CACNNNNGTG 1 cut(s) 577
AluBI AGCT 6 cut(s) 33, 172, 203, 242, 361, 675
AluI AGCT 6 cut(s) 33, 172, 203, 242, 361, 675
Alw21I GWGCWC 2 cut(s) 572, 623
AoxI GGCC 2 cut(s) 263, 337
ApaI GGGCCC 1 cut(s) 341
ApeKI GCWGC 3 cut(s) 30, 203, 675
ApoI RAATTY 1 cut(s) 125
AspS9I GGNCC 3 cut(s) 337, 338, 422
AsuII TTCGAA 1 cut(s) 487
AvaII GGWCC 1 cut(s) 422
BaeGI GKGCMC 1 cut(s) 341
BanII GRGCYC 2 cut(s) 341, 630
BbsI GAAGAC 1 cut(s) 337
Bbv12I GWGCWC 2 cut(s) 572, 623
BbvI GCAGC 3 cut(s) 42, 190, 662
BccI CCATC 3 cut(s) 91, 595, 661
BfaI CTAG 2 cut(s) 5, 653
BfrI CTTAAG 2 cut(s) 259, 362
BisI GCNGC 3 cut(s) 31, 204, 676
BlsI GCNGC 3 cut(s) 32, 205, 677
BmcAI AGTACT 1 cut(s) 404
Bme18I GGWCC 1 cut(s) 422
BmgT120I GGNCC 3 cut(s) 337, 338, 422
BmiI GGNNCC 1 cut(s) 339
BmrI ACTGGG 1 cut(s) 335
BmsI GCATC 2 cut(s) 211, 516
BmuI ACTGGG 1 cut(s) 335
BpiI GAAGAC 1 cut(s) 337
Bpu14I TTCGAA 1 cut(s) 487
BsaJI CCNNGG 2 cut(s) 81, 319
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 667
Bse1I ACTGG 2 cut(s) 52, 341
Bse3DI GCAATG 2 cut(s) 505, 540
BseDI CCNNGG 2 cut(s) 81, 319
BseGI GGATG 2 cut(s) 511, 531
BseLI CCNNNNNNNGG 2 cut(s) 145, 667
BseMI GCAATG 2 cut(s) 505, 540
BseMII CTCAG 1 cut(s) 323
BseNI ACTGG 2 cut(s) 52, 341
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 1 cut(s) 341
BseXI GCAGC 3 cut(s) 42, 190, 662
BshFI GGCC 2 cut(s) 265, 339
BsiHKAI GWGCWC 2 cut(s) 572, 623
BslI CCNNNNNNNGG 2 cut(s) 145, 667
BsnI GGCC 2 cut(s) 265, 339
Bsp119I TTCGAA 1 cut(s) 487
Bsp120I GGGCCC 1 cut(s) 337
Bsp1286I GDGCHC 4 cut(s) 341, 572, 623, 630
Bsp1407I TGTACA 1 cut(s) 581
Bsp143I GATC 1 cut(s) 268
BspACI CCGC 1 cut(s) 101
BspANI GGCC 2 cut(s) 265, 339
BspCNI CTCAG 1 cut(s) 322
BspLI GGNNCC 1 cut(s) 339
BspT104I TTCGAA 1 cut(s) 487
BspTI CTTAAG 2 cut(s) 259, 362
BsrDI GCAATG 2 cut(s) 505, 540
BsrGI TGTACA 1 cut(s) 581
BsrI ACTGG 2 cut(s) 52, 341
BssECI CCNNGG 2 cut(s) 81, 319
BssMI GATC 1 cut(s) 268
BssT1I CCWWGG 2 cut(s) 81, 319
Bst4CI ACNGT 3 cut(s) 167, 482, 694
Bst6I CTCTTC 1 cut(s) 150
BstAFI CTTAAG 2 cut(s) 259, 362
BstAUI TGTACA 1 cut(s) 581
BstBI TTCGAA 1 cut(s) 487
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 2 cut(s) 309, 470
BstF5I GGATG 2 cut(s) 511, 531
BstKTI GATC 1 cut(s) 271
BstMBI GATC 1 cut(s) 268
BstMWI GCNNNNNNNGC 5 cut(s) 10, 98, 107, 209, 440
BstSLI GKGCMC 1 cut(s) 341
BstV1I GCAGC 3 cut(s) 42, 190, 662
BstV2I GAAGAC 1 cut(s) 337
BstXI CCANNNNNNTGG 3 cut(s) 52, 118, 419
BsuRI GGCC 2 cut(s) 265, 339
BtsCI GGATG 2 cut(s) 511, 531
BtsI GCAGTG 1 cut(s) 278
BtsIMutI CAGTG 2 cut(s) 45, 278
Cac8I GCNNGC 1 cut(s) 531
Cfr13I GGNCC 3 cut(s) 337, 338, 422
Csp6I GTAC 3 cut(s) 163, 403, 582
CviAII CATG 4 cut(s) 328, 413, 522, 548
CviQI GTAC 3 cut(s) 163, 403, 582
DdeI CTNAG 2 cut(s) 309, 470
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EciI GGCGGA 1 cut(s) 116
Eco130I CCWWGG 2 cut(s) 81, 319
Eco24I GRGCYC 2 cut(s) 341, 630
Eco47I GGWCC 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 337
EcoT14I CCWWGG 2 cut(s) 81, 319
EcoT38I GRGCYC 2 cut(s) 341, 630
ErhI CCWWGG 2 cut(s) 81, 319
FaeI CATG 4 cut(s) 331, 416, 525, 551
FatI CATG 4 cut(s) 327, 412, 521, 547
Fnu4HI GCNGC 3 cut(s) 31, 204, 676
FokI GGATG 2 cut(s) 518, 538
FriOI GRGCYC 2 cut(s) 341, 630
Fsp4HI GCNGC 3 cut(s) 31, 204, 676
FspBI CTAG 2 cut(s) 5, 653
GluI GCNGC 3 cut(s) 31, 204, 676
HaeIII GGCC 2 cut(s) 265, 339
Hin1II CATG 4 cut(s) 331, 416, 525, 551
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 1 cut(s) 698
HpaI GTTAAC 1 cut(s) 544
Hpy166II GTNNAC 3 cut(s) 544, 582, 584
Hpy188I TCNGA 4 cut(s) 312, 615, 690, 703
Hpy188III TCNNGA 1 cut(s) 638
Hpy8I GTNNAC 3 cut(s) 544, 582, 584
Hpy99I CGWCG 1 cut(s) 694
HpyCH4III ACNGT 3 cut(s) 167, 482, 694
HpyCH4IV ACGT 1 cut(s) 185
HpyCH4V TGCA 7 cut(s) 30, 212, 327, 455, 521, 533, 633
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 98, 107, 209, 440
HpyF3I CTNAG 2 cut(s) 309, 470
HpySE526I ACGT 1 cut(s) 185
Hsp92II CATG 4 cut(s) 331, 416, 525, 551
KspAI GTTAAC 1 cut(s) 544
Kzo9I GATC 1 cut(s) 268
LmnI GCTCC 1 cut(s) 177
LpnPI CCDG 9 cut(s) 33, 46, 152, 279, 354, 560, 609, 651, 677
Lsp1109I GCAGC 3 cut(s) 42, 190, 662
LweI GCATC 2 cut(s) 211, 516
MaeI CTAG 2 cut(s) 5, 653
MaeII ACGT 1 cut(s) 185
MaeIII GTNAC 1 cut(s) 508
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MboII GAAGA 4 cut(s) 50, 167, 187, 337
MfeI CAATTG 1 cut(s) 513
MhlI GDGCHC 4 cut(s) 341, 572, 623, 630
MluCI AATT 3 cut(s) 125, 513, 647
MnlI CCTC 3 cut(s) 109, 151, 496
MseI TTAA 4 cut(s) 237, 260, 363, 543
MslI CAYNNNNRTG 2 cut(s) 417, 577
MspA1I CMGCKG 1 cut(s) 675
MspCI CTTAAG 2 cut(s) 259, 362
MunI CAATTG 1 cut(s) 513
MwoI GCNNNNNNNGC 5 cut(s) 10, 98, 107, 209, 440
NdeII GATC 1 cut(s) 268
NlaIII CATG 4 cut(s) 331, 416, 525, 551
NlaIV GGNNCC 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 508
NspV TTCGAA 1 cut(s) 487
OliI CACNNNNGTG 1 cut(s) 577
PfeI GAWTC 1 cut(s) 698
PflMI CCANNNNNTGG 1 cut(s) 667
PkrI GCNGC 3 cut(s) 32, 205, 677
Psp1406I AACGTT 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 339
PspOMI GGGCCC 1 cut(s) 337
PspPI GGNCC 3 cut(s) 337, 338, 422
PvuII CAGCTG 1 cut(s) 675
RsaI GTAC 3 cut(s) 164, 404, 583
RsaNI GTAC 3 cut(s) 163, 403, 582
RseI CAYNNNNRTG 2 cut(s) 417, 577
SaqAI TTAA 4 cut(s) 237, 260, 363, 543
SatI GCNGC 3 cut(s) 31, 204, 676
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 3 cut(s) 337, 338, 422
ScaI AGTACT 1 cut(s) 404
SduI GDGCHC 4 cut(s) 341, 572, 623, 630
SfaNI GCATC 2 cut(s) 211, 516
SfuI TTCGAA 1 cut(s) 487
SinI GGWCC 1 cut(s) 422
SmiMI CAYNNNNRTG 2 cut(s) 417, 577
SmlI CTYRAG 2 cut(s) 259, 362
SmoI CTYRAG 2 cut(s) 259, 362
Sse9I AATT 3 cut(s) 125, 513, 647
SsiI CCGC 1 cut(s) 101
SspMI CTAG 2 cut(s) 5, 653
StyI CCWWGG 2 cut(s) 81, 319
TaaI ACNGT 3 cut(s) 167, 482, 694
TaiI ACGT 1 cut(s) 188
TaqI TCGA 1 cut(s) 487
TasI AATT 3 cut(s) 125, 513, 647
TatI WGTACW 3 cut(s) 162, 402, 581
TfiI GAWTC 1 cut(s) 698
Tru1I TTAA 4 cut(s) 237, 260, 363, 543
Tru9I TTAA 4 cut(s) 237, 260, 363, 543
TscAI CASTG 2 cut(s) 52, 285
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 3 cut(s) 30, 203, 675
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 2 cut(s) 344, 551
TspRI CASTG 2 cut(s) 52, 285
Van91I CCANNNNNTGG 1 cut(s) 667
Vha464I CTTAAG 2 cut(s) 259, 362
VpaK11BI GGWCC 1 cut(s) 422
XapI RAATTY 1 cut(s) 125
XspI CTAG 2 cut(s) 5, 653
ZrmI AGTACT 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.