MD03G1246200.v1.1

BES1 BZR1 homolog protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009842
Physical Location & Seq
Forward (+)
33321839 .. 33325676
3838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1246200.v1.1.491

Sequence Viewer

Length: 975 bp
ATGACGTCGGGGACGCGATTGCCGACGTCGAAGGAGAGGGAAAACAACAAGAGGAGAGAGAGGAGGCGGAGGGCCATAGCGGCGAAGATATTTGCGGGCCTGAGGATGTATGGAAACTACAAGCTCCCCAAGCACTGCGACAACAATGAGGTCCTCAAGGCTCTCTGCGACGAGGCCGGTTGGACCGTCGAATTGGACGGTACCACTTACCGCAAGGGATGCAAGCCGGTCGAACGTATGGAAGTGATGGCTGGATCCACAGTAGCTAGCCCGAGCTCGTCTTTCCACCCAAGTAATTGTGCTTCGTACAATCCTAGCCCAGGCTCTTCCTCCTTCCCAAGCCCAACATCATCTTCCTATGCTGCTAATCAAAATGCTGATAGCAGATCCCTTATCCCATGGCTCAGAAACCTCTCATCTGCATCCTCGTCAGCCTTCTCTTCCAAACTACCAAACCTCTACATCCACAGCGGCTCTATCAGTGCTCCTGTTACCCCTCCATTAAGCTCCCCAACTGCCAGGACGCCGAGAATGAGAACTGACTGGGATGACCAGTCTGCTCGCCCAGGTTGGGCTGCACAGAAATACCCCTTCCTGCCATCTTCTACTCCACCAAGCCCTGGACGGCAGATTGTTCCTGATCCAGAATGGTTTGCTGGGATTCCGATTCCCCATGGACCAACTTCTCCAACATTCAGCCTTGTCTCTTCAAACCCATTTGGGTTTAAGGACGAGGTTTCAGCCGGTGGTGGATCCCGCATGTGGACTCCAGGGCAAAGTGGGACTTGTTCTCCTGCCATTGCAGCTGGCTCCGATCACGCTGCAGACATCCCAATGTCTGAGGTGATTTCTGATGAGTTTGCATTTGGATGCAATACAACAGGGCTGGTTAAGGCATGGGAAGGAGAGAAGATTCACGAAGACTCTGGATCCGATGATCTGGAGCTCACTCTTGGGAGCTCAAGAACAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BES1_N

Protein Analysis

325

Amino Acids

34.79

Weight (kDa)

8.5

Isoelectric Point (pI)

57.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BES1_N PF05687 4 - 139 2.4e-58 BES1/BZR1 plant transcription factor, N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 8, 29
Acc65I GGTACC 1 cut(s) 200
AccB1I GGYRCC 1 cut(s) 200
AccB7I CCANNNNNTGG 1 cut(s) 620
AccII CGCG 1 cut(s) 16
AciI CCGC 6 cut(s) 67, 80, 95, 211, 471, 757
AclWI GGATC 8 cut(s) 249, 262, 381, 635, 747, 760, 924, 937
AcyI GRCGYC 3 cut(s) 5, 26, 524
AfaI GTAC 2 cut(s) 202, 308
AfiI CCNNNNNNNGG 4 cut(s) 320, 571, 620, 762
AgsI TTSAA 1 cut(s) 711
AjnI CCWGG 5 cut(s) 319, 518, 565, 619, 769
AjuI GAANNNNNNNTTGG 2 cut(s) 337, 369
AluBI AGCT 7 cut(s) 124, 266, 276, 507, 806, 946, 960
AluI AGCT 7 cut(s) 124, 266, 276, 507, 806, 946, 960
Alw21I GWGCWC 4 cut(s) 278, 487, 948, 962
Alw26I GTCTC 1 cut(s) 709
AlwI GGATC 8 cut(s) 249, 262, 381, 635, 747, 760, 924, 937
Ama87I CYCGRG 1 cut(s) 271
AoxI GGCC 3 cut(s) 72, 97, 174
ApeKI GCWGC 4 cut(s) 362, 575, 803, 821
Asp718I GGTACC 1 cut(s) 200
AspS9I GGNCC 5 cut(s) 72, 97, 151, 183, 677
AsuHPI GGTGA 1 cut(s) 856
AsuNHI GCTAGC 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 271
AvaII GGWCC 3 cut(s) 151, 183, 677
AxyI CCTNAGG 1 cut(s) 101
BamHI GGATCC 3 cut(s) 254, 752, 929
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 3 cut(s) 278, 948, 962
BarI GAAGNNNNNNTAC 2 cut(s) 286, 318
BbsI GAAGAC 1 cut(s) 927
Bbv12I GWGCWC 4 cut(s) 278, 487, 948, 962
BbvI GCAGC 4 cut(s) 349, 562, 808, 815
BccI CCATC 2 cut(s) 241, 607
BceAI ACGGC 1 cut(s) 641
BcgI CGANNNNNNTGC 4 cut(s) 211, 245, 803, 837
BciT130I CCWGG 5 cut(s) 321, 520, 567, 621, 771
BcoDI GTCTC 1 cut(s) 709
BfaI CTAG 2 cut(s) 267, 315
BfmI CTRYAG 1 cut(s) 822
BglI GCCNNNNNGGC 1 cut(s) 80
BisI GCNGC 6 cut(s) 81, 363, 472, 576, 804, 822
BlsI GCNGC 6 cut(s) 82, 364, 473, 577, 805, 823
Bme1390I CCNGG 5 cut(s) 321, 520, 567, 621, 771
Bme18I GGWCC 3 cut(s) 151, 183, 677
BmeT110I CYCGRG 1 cut(s) 271
BmgT120I GGNCC 5 cut(s) 72, 97, 151, 183, 677
BmiI GGNNCC 5 cut(s) 202, 256, 754, 811, 931
BmrFI CCNGG 5 cut(s) 321, 520, 567, 621, 771
BmrI ACTGGG 1 cut(s) 553
BmsI GCATC 3 cut(s) 209, 431, 860
BmtI GCTAGC 1 cut(s) 270
BmuI ACTGGG 1 cut(s) 553
BpiI GAAGAC 1 cut(s) 927
BpmI CTGGAG 2 cut(s) 753, 962
BpuEI CTTGAG 2 cut(s) 140, 946
BsaHI GRCGYC 3 cut(s) 5, 26, 524
BsaJI CCNNGG 6 cut(s) 319, 398, 565, 619, 673, 770
BsaXI ACNNNNNCTCC 2 cut(s) 775, 805
Bsc4I CCNNNNNNNGG 4 cut(s) 320, 571, 620, 762
Bse118I RCCGGY 3 cut(s) 176, 226, 743
Bse1I ACTGG 2 cut(s) 548, 553
Bse21I CCTNAGG 1 cut(s) 101
Bse3DI GCAATG 1 cut(s) 798
BseBI CCWGG 5 cut(s) 321, 520, 567, 621, 771
BseDI CCNNGG 6 cut(s) 319, 398, 565, 619, 673, 770
BseGI GGATG 7 cut(s) 111, 224, 422, 462, 553, 828, 875
BseLI CCNNNNNNNGG 4 cut(s) 320, 571, 620, 762
BseMI GCAATG 1 cut(s) 798
BseMII CTCAG 3 cut(s) 92, 418, 831
BseNI ACTGG 2 cut(s) 548, 553
BseRI GAGGAG 2 cut(s) 67, 76
BseXI GCAGC 4 cut(s) 349, 562, 808, 815
BseYI CCCAGC 1 cut(s) 656
BsgI GTGCAG 1 cut(s) 561
Bsh1236I CGCG 1 cut(s) 16
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 3 cut(s) 74, 99, 176
BshNI GGYRCC 1 cut(s) 200
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 4 cut(s) 278, 487, 948, 962
BsiHKCI CYCGRG 1 cut(s) 271
BsiSI CCGG 3 cut(s) 177, 227, 744
BslFI GGGAC 2 cut(s) 25, 796
BslI CCNNNNNNNGG 4 cut(s) 320, 571, 620, 762
BsmAI GTCTC 1 cut(s) 709
BsmFI GGGAC 2 cut(s) 25, 796
BsnI GGCC 3 cut(s) 74, 99, 176
BsoBI CYCGRG 1 cut(s) 271
Bsp1286I GDGCHC 4 cut(s) 278, 487, 948, 962
Bsp143I GATC 7 cut(s) 254, 386, 640, 752, 814, 929, 937
Bsp19I CCATGG 2 cut(s) 398, 673
BspACI CCGC 6 cut(s) 67, 80, 95, 211, 471, 757
BspANI GGCC 3 cut(s) 74, 99, 176
BspCNI CTCAG 3 cut(s) 93, 417, 832
BspFNI CGCG 1 cut(s) 16
BspLI GGNNCC 5 cut(s) 202, 256, 754, 811, 931
BspMAI CTGCAG 1 cut(s) 826
BspOI GCTAGC 1 cut(s) 270
BspPI GGATC 8 cut(s) 249, 262, 381, 635, 747, 760, 924, 937
BspQI GCTCTTC 1 cut(s) 331
BspT107I GGYRCC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 798
BsrFI RCCGGY 3 cut(s) 176, 226, 743
BsrI ACTGG 2 cut(s) 548, 553
BssAI RCCGGY 3 cut(s) 176, 226, 743
BssECI CCNNGG 6 cut(s) 319, 398, 565, 619, 673, 770
BssMI GATC 7 cut(s) 254, 386, 640, 752, 814, 929, 937
BssNI GRCGYC 3 cut(s) 5, 26, 524
BssT1I CCWWGG 2 cut(s) 398, 673
Bst2UI CCWGG 5 cut(s) 321, 520, 567, 621, 771
Bst4CI ACNGT 3 cut(s) 187, 200, 262
Bst6I CTCTTC 3 cut(s) 331, 445, 712
BstACI GRCGYC 3 cut(s) 5, 26, 524
BstAPI GCANNNNNTGC 1 cut(s) 219
BstC8I GCNNGC 5 cut(s) 97, 224, 268, 562, 808
BstDEI CTNAG 3 cut(s) 101, 404, 840
BstDSI CCRYGG 2 cut(s) 398, 673
BstENI CCTNNNNNAGG 1 cut(s) 318
BstF5I GGATG 7 cut(s) 111, 224, 422, 462, 553, 828, 875
BstFNI CGCG 1 cut(s) 16
BstKTI GATC 7 cut(s) 257, 389, 643, 755, 817, 932, 940
BstMAI GTCTC 1 cut(s) 709
BstMBI GATC 7 cut(s) 254, 386, 640, 752, 814, 929, 937
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 4 cut(s) 80, 130, 219, 803
BstNI CCWGG 5 cut(s) 321, 520, 567, 621, 771
BstNSI RCATGY 1 cut(s) 763
BstSCI CCNGG 5 cut(s) 319, 518, 565, 619, 769
BstSFI CTRYAG 1 cut(s) 822
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 4 cut(s) 349, 562, 808, 815
BstV2I GAAGAC 1 cut(s) 927
BstX2I RGATCY 4 cut(s) 254, 386, 752, 929
BstYI RGATCY 4 cut(s) 254, 386, 752, 929
Bsu36I CCTNAGG 1 cut(s) 101
BsuRI GGCC 3 cut(s) 74, 99, 176
BtgI CCRYGG 2 cut(s) 398, 673
BtsCI GGATG 7 cut(s) 111, 224, 422, 462, 553, 828, 875
BtsI GCAGTG 1 cut(s) 133
BtsIMutI CAGTG 2 cut(s) 133, 487
Cac8I GCNNGC 5 cut(s) 97, 224, 268, 562, 808
Cfr10I RCCGGY 3 cut(s) 176, 226, 743
Cfr13I GGNCC 5 cut(s) 72, 97, 151, 183, 677
CseI GACGC 2 cut(s) 22, 532
Csp6I GTAC 2 cut(s) 201, 307
CviAII CATG 4 cut(s) 399, 674, 760, 897
CviQI GTAC 2 cut(s) 201, 307
DdeI CTNAG 3 cut(s) 101, 404, 840
DpnI GATC 7 cut(s) 256, 388, 642, 754, 816, 931, 939
DpnII GATC 7 cut(s) 254, 386, 640, 752, 814, 929, 937
Eam1104I CTCTTC 3 cut(s) 331, 445, 712
EarI CTCTTC 3 cut(s) 331, 445, 712
EciI GGCGGA 1 cut(s) 82
Ecl136II GAGCTC 3 cut(s) 276, 946, 960
Eco130I CCWWGG 2 cut(s) 398, 673
Eco24I GRGCYC 3 cut(s) 278, 948, 962
Eco47I GGWCC 3 cut(s) 151, 183, 677
Eco53kI GAGCTC 3 cut(s) 276, 946, 960
Eco81I CCTNAGG 1 cut(s) 101
Eco88I CYCGRG 1 cut(s) 271
EcoICRI GAGCTC 3 cut(s) 276, 946, 960
EcoNI CCTNNNNNAGG 1 cut(s) 318
EcoO109I RGGNCCY 1 cut(s) 151
EcoRII CCWGG 5 cut(s) 319, 518, 565, 619, 769
EcoT14I CCWWGG 2 cut(s) 398, 673
EcoT38I GRGCYC 3 cut(s) 278, 948, 962
ErhI CCWWGG 2 cut(s) 398, 673
FaeI CATG 4 cut(s) 402, 677, 763, 900
FaiI YATR 8 cut(s) 77, 111, 239, 360, 400, 675, 761, 898
FalI AAGNNNNNCTT 2 cut(s) 769, 801
FaqI GGGAC 2 cut(s) 25, 796
FatI CATG 4 cut(s) 398, 673, 759, 896
FauI CCCGC 2 cut(s) 88, 764
Fnu4HI GCNGC 6 cut(s) 81, 363, 472, 576, 804, 822
FokI GGATG 7 cut(s) 118, 231, 409, 449, 560, 815, 882
FriOI GRGCYC 3 cut(s) 278, 948, 962
Fsp4HI GCNGC 6 cut(s) 81, 363, 472, 576, 804, 822
FspBI CTAG 2 cut(s) 267, 315
GluI GCNGC 6 cut(s) 81, 363, 472, 576, 804, 822
GsaI CCCAGC 1 cut(s) 660
GsuI CTGGAG 2 cut(s) 753, 962
HaeIII GGCC 3 cut(s) 74, 99, 176
HapII CCGG 3 cut(s) 177, 227, 744
HgaI GACGC 2 cut(s) 22, 532
Hin1I GRCGYC 3 cut(s) 5, 26, 524
Hin1II CATG 4 cut(s) 402, 677, 763, 900
HinfI GANTC 5 cut(s) 661, 667, 766, 913, 923
HpaII CCGG 3 cut(s) 177, 227, 744
HphI GGTGA 1 cut(s) 856
Hpy166II GTNNAC 1 cut(s) 765
Hpy188I TCNGA 6 cut(s) 407, 666, 814, 841, 853, 934
Hpy188III TCNNGA 6 cut(s) 638, 644, 917, 927, 941, 963
Hpy8I GTNNAC 1 cut(s) 765
Hpy99I CGWCG 5 cut(s) 10, 28, 31, 173, 191
HpyAV CCTTC 5 cut(s) 25, 343, 445, 601, 896
HpyCH4III ACNGT 3 cut(s) 187, 200, 262
HpyCH4IV ACGT 3 cut(s) 5, 26, 235
HpyCH4V TGCA 7 cut(s) 222, 422, 578, 803, 824, 863, 873
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 130, 219, 803
HpyF3I CTNAG 3 cut(s) 101, 404, 840
HpySE526I ACGT 3 cut(s) 5, 26, 235
Hsp92I GRCGYC 3 cut(s) 5, 26, 524
Hsp92II CATG 4 cut(s) 402, 677, 763, 900
KpnI GGTACC 1 cut(s) 204
Kzo9I GATC 7 cut(s) 254, 386, 640, 752, 814, 929, 937
LguI GCTCTTC 1 cut(s) 331
LmnI GCTCC 6 cut(s) 129, 490, 512, 815, 943, 957
Lsp1109I GCAGC 4 cut(s) 349, 562, 808, 815
LweI GCATC 3 cut(s) 209, 431, 860
MaeI CTAG 2 cut(s) 267, 315
MaeII ACGT 3 cut(s) 5, 26, 235
MaeIII GTNAC 1 cut(s) 490
MalI GATC 7 cut(s) 256, 388, 642, 754, 816, 931, 939
MboI GATC 7 cut(s) 254, 386, 640, 752, 814, 929, 937
MboII GAAGA 8 cut(s) 97, 318, 345, 432, 594, 699, 922, 932
MflI RGATCY 4 cut(s) 254, 386, 752, 929
MhlI GDGCHC 4 cut(s) 278, 487, 948, 962
MluCI AATT 2 cut(s) 191, 295
MlyI GAGTC 2 cut(s) 760, 917
MmeI TCCRAC 2 cut(s) 161, 713
MseI TTAA 3 cut(s) 503, 726, 891
MslI CAYNNNNRTG 2 cut(s) 833, 868
MspA1I CMGCKG 2 cut(s) 471, 806
MspI CCGG 3 cut(s) 177, 227, 744
MspR9I CCNGG 5 cut(s) 321, 520, 567, 621, 771
MvaI CCWGG 5 cut(s) 321, 520, 567, 621, 771
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 4 cut(s) 80, 130, 219, 803
NcoI CCATGG 2 cut(s) 398, 673
NdeII GATC 7 cut(s) 254, 386, 640, 752, 814, 929, 937
NheI GCTAGC 1 cut(s) 266
NlaIII CATG 4 cut(s) 402, 677, 763, 900
NlaIV GGNNCC 5 cut(s) 202, 256, 754, 811, 931
NmeAIII GCCGAG 1 cut(s) 552
NspI RCATGY 1 cut(s) 763
PciSI GCTCTTC 1 cut(s) 331
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PfeI GAWTC 3 cut(s) 661, 667, 913
PflMI CCANNNNNTGG 1 cut(s) 620
PkrI GCNGC 6 cut(s) 82, 364, 473, 577, 805, 823
PleI GAGTC 2 cut(s) 760, 917
PpsI GAGTC 2 cut(s) 760, 917
PpuMI RGGWCCY 1 cut(s) 151
Psp124BI GAGCTC 3 cut(s) 278, 948, 962
Psp5II RGGWCCY 1 cut(s) 151
Psp6I CCWGG 5 cut(s) 319, 518, 565, 619, 769
PspFI CCCAGC 1 cut(s) 656
PspGI CCWGG 5 cut(s) 319, 518, 565, 619, 769
PspN4I GGNNCC 5 cut(s) 202, 256, 754, 811, 931
PspPI GGNCC 5 cut(s) 72, 97, 151, 183, 677
PspPPI RGGWCCY 1 cut(s) 151
PstI CTGCAG 1 cut(s) 826
PsuI RGATCY 4 cut(s) 254, 386, 752, 929
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 2 cut(s) 202, 308
RsaNI GTAC 2 cut(s) 201, 307
RseI CAYNNNNRTG 2 cut(s) 833, 868
SacI GAGCTC 3 cut(s) 278, 948, 962
SapI GCTCTTC 1 cut(s) 331
SaqAI TTAA 3 cut(s) 503, 726, 891
SatI GCNGC 6 cut(s) 81, 363, 472, 576, 804, 822
Sau3AI GATC 7 cut(s) 254, 386, 640, 752, 814, 929, 937
Sau96I GGNCC 5 cut(s) 72, 97, 151, 183, 677
SchI GAGTC 2 cut(s) 760, 917
ScrFI CCNGG 5 cut(s) 321, 520, 567, 621, 771
SduI GDGCHC 4 cut(s) 278, 487, 948, 962
SfaNI GCATC 3 cut(s) 209, 431, 860
SfcI CTRYAG 1 cut(s) 822
SinI GGWCC 3 cut(s) 151, 183, 677
SmiMI CAYNNNNRTG 2 cut(s) 833, 868
SmlI CTYRAG 2 cut(s) 155, 961
SmoI CTYRAG 2 cut(s) 155, 961
Sse9I AATT 2 cut(s) 191, 295
SsiI CCGC 6 cut(s) 67, 80, 95, 211, 471, 757
SspMI CTAG 2 cut(s) 267, 315
SstI GAGCTC 3 cut(s) 278, 948, 962
StyD4I CCNGG 5 cut(s) 319, 518, 565, 619, 769
StyI CCWWGG 2 cut(s) 398, 673
TaaI ACNGT 3 cut(s) 187, 200, 262
TaiI ACGT 3 cut(s) 8, 29, 238
TaqI TCGA 3 cut(s) 29, 189, 231
TasI AATT 2 cut(s) 191, 295
TauI GCSGC 2 cut(s) 83, 474
TfiI GAWTC 3 cut(s) 661, 667, 913
Tru1I TTAA 3 cut(s) 503, 726, 891
Tru9I TTAA 3 cut(s) 503, 726, 891
TscAI CASTG 2 cut(s) 140, 487
TseI GCWGC 4 cut(s) 362, 575, 803, 821
TspRI CASTG 2 cut(s) 140, 487
Van91I CCANNNNNTGG 1 cut(s) 620
VpaK11BI GGWCC 3 cut(s) 151, 183, 677
XagI CCTNNNNNAGG 1 cut(s) 318
XceI RCATGY 1 cut(s) 763
XspI CTAG 2 cut(s) 267, 315
ZraI GACGTC 2 cut(s) 6, 27
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.