Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0011732

Physical Location & Seq

Genomic Coordinates Reverse (-)

35359722 .. 35367148

Gene Structure 7427 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1269000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4023 bp

ATGCAACCCCAATTTCCTCCGACGATGATGACGACGATGGCCGGGTCCGTACAGCAGGCGCCGCCGAGTCAGCTGTGGGACTCGGTGCTCCAGCTGACCAAGTCCGCCCAGGACAAGAACAGTGACCCGCTCCTCTGGGCGGTCCAGCTCAGCTCCAGCCTCACCTCCTCCGCCGTCTCCCTGCCCTCCGTCGAGCTCGCCCACCTCCTCGTCTCCCACATCTGCTGGGCCAATCACGTGCCCATCACGTGGAAGTTCCTCGAGAAGGCACTGACGTTCAAGATCGTCCCCCCCATGCTCGTCCTTGCTCTGCTCTCCACCAAGGTTGTTCCAAATCGACAGCTTCACCCTACAGCATATAGGCTCTACTTGGAACTTCTTAAAAGACATTCATTTTTATTTGCTTCTCAATTGAATGGACCAAATAGTCAGAAGATTATAAAATCCATTGATGATGTTCTACATCTTTCCCGGCAGTATGGCCTTCAAGTGTCTGAACATGGAGTTCTTATTGTTGAGTTTGTCTTTTCAATCATATGGCAGTTACTTGATGCATCCTTAGATGATGAAGGTTTGCTGGAACTTGCCCCAGATAAGAAGCCTAGATGGCCTACCAGGCCACAAGATATGGAAATAGATGGTCATGGTTGCTTTAATGAGACGAGAAGTGAGCAAAATGAAGGTTTGCAGAAAGCAAATACTGCAATGGCTATTGACATAATTGTGGAGTTTCTGCAGAACAAAGTAACTTCAAGGATGTTGTACTTAGCCCGTCGAAATATGCCATCACACTGGGGAGGTTTCGTTCAGAAATTGCAAGTGCTTGCGGCCAACTCATCAGTTTTGAGGACTATGAAACATATAAACCCAGAGGGTCTTCTACAGCTGACATCTGATACCCGTCGACTTCTGACTCGGGAATGCAAAACAATTTCACGACAAGAATTTCATGCAGTCTTGTCTTCTGGGTCTTTAATGTCCTCTGCCTGTCAGTCTCACGGAATGAGTTCATCTGCTTTTTGGCTTCCCATTGATCTGTTTCTTGAAGATGCTATGGATGGATCAGAAGCAGCTGAAATTAGTGCCGTCGAATCCCTTACTGGTTTAGTGAAGGCTCTGCAGGCAGTTAATGCCACCACATGGCACAATGCATTTTTAGGTTTATGGATTTCAGCTCTTCGGCTGGTTCAAAGGGAAAGGGATCCTAGAGAAGGACCTGTCCCTCGTCTTGATACCTGCTTATGCATGTTGTTGAGTATTACGACGCTTGCAGTTACTAATATTATTGAAGAGGAAGCTGAATTGATGGAGGAAACGGAGGGGGATCATACTAACCAAAGGAAGGAACAGGCTCCTGGAAAGCGCCGCAAAGATTTAGTTAGCTGCTTACAGCGATTGGGTGATTATGAGACCTTGTTGACTCCACCTCAGTCTGTTTGTTCAGTGGTGAATCAAGCTGCAGCTAAGGCTATAATGTTCAGATCAGGCCTTACAGTCAGTAATGGATACCACGAATCTGTTAGCGTGAACGACATGCCAATAAATTGTGTGGGAAACTTGCGGCATCTGATTGTTGAAGCTTGTATTGCGCGGAATATCCTAGACACATCAGCATATTTTTGGCCAGGCTATGTGAGTGCACGCAGCAACCAAGTGCTACGCAATGTGCCCAGCCAAGTGCCTTCTTGGTCTTCAATAATGAAGGGGTCACCTCTAACTCCATCACTGGGAAATGCTTTAGTTGCCACTCCAGCTTCTAGCTTAGCAGAAATTGAGAAAATATATGAGATTGCAGTCAATGGTTCAGATGAAGAGAAGATATCTGCTGCTACCATTCTTTGTGGGGCTTCTCTTGTTCGTGGATGGAATATACAGGAACACACCTGTCTTTTTATTATAAGATTGCTGTCACCTCCGGTTCCTGCAGATTACTCGGGGAGTGATAGCCATTTGATCGGCTATGCTTCATTCTTTAATGTCCTTCTGGTTGGCATATCATCTATAGATACTGTACAGATTTTCTCCTTATTGGGATTGGTTCCACTACTTGCAGCTGCATTGATGCCTATTTGTGAAGTTTTTGGCTCAAGTGTTCCCAATTTCTCATGGACTCCTACAACGGGGGAAGAACTCTCATGCCACGCAGTGTTCTCAAATGCTTTTACACTTCTATTGAGGTTGTGGAGATTTGATCATCCACCTCTTGAACACATGATGGGCGACTACCCAACTGTGGGAATCCAAACTGGTCCAGAATACCTCTTGTTGCTGCGAAACTGTCGATTAGCATCCATGGGAAATTCAGTCAGGGATCGAATAAAAAGCAGAAGAGTTTCAAAATTCATCACCTTCCCTTCAGGTACCATTACCATGGATTGCTTTCCGAGACTGAAACTCTGGTACCAGCAACATCAAAAATGTATTTGCTCTACCCTGTCTGGGCTTGTACCTGGGACTCCCGTTCATCAACTTGTTGATGCACTCCTGACCATGATGTTTAGAAAAATAAATAGTAGTCAGCAGTTGACTCCAGCAACGTCAGGTAATAGTAATTCATCTGCATCGGGAACTGATGAGTCCTGTATTAGACTCAAGGTACCAGCCTGGGATATCCTTGAAGCAACTCCATTTGTCCTTGATGCTGCTCTTACTGCATGCGCCCATGGAAGACTATCTCCCCGTGAACTGGCTACAGGACTGAAAGATCTCGCTGATTATCTTCCAGCGACTTTGGGAACCATGGTAAGCTATTTTTCAGCTGAAGTAACACGGGGAATATGGAAGCCAGCTTGTATGAACGGAACTGATTGGCCAAGCCCTGCCGCAAATTTACTACTTGTTGAGCAGCAAATAAAGAAAATCCTAGCTGCCACTGGTGTTGATGTCCCTAGCCTCTCAGTTGGTGGGAGCGCCCCAGCTACGCTTCCCTTACCATTTGCAGCCCTCATAAGTCTCACAATAACTTATAAACTTGATAGAGCCTCTGAACGTGCCCTTCATCTGATTGGACCGGCCATGAACTCCCTAGCTGCTGGTTGCCCATGGCCATGCATGCCAATCCTAGCCTCATTGTGGGCCCAGAAGGTAAAGCGCTGGAGTGACTTCCTGGTGTTTTCTGCTTCTCAAACTGTCTTCCACCACAACAGTGATGCTGTGGTCCAGCTTCTTAAGAGCTGCTTTACATCTACTCTCGGGTTAAGTTCCTCTCGTGCTTACAGCAACGGCAGTGTGGGTGCCCTCCTTGGTCATGGCTTTGGTTCCCATTTGTCTGGTGGGATTTCTCCTGTTGCCCCCGGAATTCTTTACTTGCGTGTGCACCGGTCTGTCAGAGATGTAATGTTCCTGACAGAAGAGGTGCTCTCTCTTCTAATGTATTCTGTTAGAGATATTGCAAATTGCGGGTTTCCAAGAGAGAGGGTGGAGAAATTGAGGAAGACCAAGCATGGGACGAGGTATGGACAGGTTTCTCTTGCTGCCGCAATGGCACGCGTCAAGATTGCAGCTACACTTGGGGCCACTTTAGTTTGGATATCTGGCGGATCGAGTTTAGTTCAATCCTTGATCAAAGAAACTTTACCTTCTTGGTTTATATCGACCCGTGGTTTGGATCAGGAAGGCAGAGAAACTGGAGGAATGGTTGCCATGTTGGGAGGTTATGCCCTTGCATACTTTGCAGTACTTTGTGGGACGTTTGCTTGGGGTGTGGATTCAGTATCACCAGCATCAAAGCGGCGACCAATGGTTCTCGGGCGGCATTTGGGATTTATTGCAGGCGCGCTGGACGGGAAAATATCACTGGGTTGTGATTGGGCTATGTGGCGGGCATATGTGTCAGGATTTATCAGTTTGATGGTGTCATGCACGCAGAAGTGGGTGCTGGAAGTTGATGTGGAAATATTGAAGATGCTGAGCAAGGGACTTAGACGGTGGAATGAGGAAGAACTGGCTCTGGCCCTGCTGGGACTCGGTGGCGTTGAGGCCATGGGCGCAGCAGCTGAACTAGTCGTTGAATGTGGGGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006725 GO:0008150 GO:0008152 GO:0009698 GO:0009987 GO:0016020 GO:0016592 GO:0019222 GO:0019748 GO:0031323 GO:0031974 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043455 GO:0044237 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0050789 GO:0050794 GO:0065007 GO:0070013 GO:0071704 GO:1901360 GO:2000762

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

1341

Amino Acids

146.33

Weight (kDa)

7.53

Isoelectric Point (pI)

47.03

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011732)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G48110
fragaria_vesca	FvH4_2g23980 FvH4_2g23980
malus_domestica	MD03G1269000.v1.1 MD11G1289800.v1.1
prunus_persica	Prupe.8G245000_v2.0.a1
pyrus_communis	pycom03g21480 pycom11g25720
rosa_chinensis	RchiOBHm_Chr6g0291641
rosa_laevigata	RLG00000012100
rosa_multiflora	Rmu_co8449785.1_g000001 Rmu_sc0002226.1_g000015
rosa_roxburghii	Rroxscaffold_7G00175900
rosa_rugosa	Rorug06G0220600
rosa_samantha	Rh6AG333200 Rh6BG340000 Rh6CG346300 Rh6DG333500
rosa_wichuraiana	Rw6G029020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	440, 1898, 2967
AasI	GACNNNNNNGTC	1 cut(s)	426
Acc36I	ACCTGC	1 cut(s)	1244
Acc65I	GGTACC	3 cut(s)	2360, 2400, 2596
AccB1I	GGYRCC	5 cut(s)	58, 2360, 2400, 2596, 3233
AccB7I	CCANNNNNTGG	2 cut(s)	249, 1140
AccBSI	CCGCTC	1 cut(s)	130
AccI	GTMKAC	1 cut(s)	904
AccII	CGCG	3 cut(s)	1591, 3489, 3776
AclWI	GGATC	7 cut(s)	1069, 1196, 1209, 1332, 2319, 3547, 3615
AcoI	YGGCCR	6 cut(s)	39, 828, 1622, 2810, 3012, 3044
AcsI	RAATTY	5 cut(s)	944, 2299, 2339, 2827, 3297
AcuI	CTGAAG	2 cut(s)	2340, 2781
AcvI	CACGTG	2 cut(s)	238, 249
AcyI	GRCGYC	1 cut(s)	59
AdeI	CACNNNGTG	1 cut(s)	2146
AfaI	GTAC	8 cut(s)	51, 764, 2013, 2362, 2402, 2448, 2598, 3678
AfeI	AGCGCT	1 cut(s)	3092
AflII	CTTAAG	1 cut(s)	3167
AflIII	ACRYGT	1 cut(s)	3487
AgeI	ACCGGT	1 cut(s)	3318
AhlI	ACTAGT	1 cut(s)	4000
AjnI	CCWGG	7 cut(s)	108, 614, 1354, 1624, 2449, 2603, 3105
AleI	CACNNNNGTG	1 cut(s)	3144
AloI	GAACNNNNNNTCC	2 cut(s)	789, 821
Alw21I	GWGCWC	5 cut(s)	90, 198, 1642, 3318, 3360
Alw26I	GTCTC	7 cut(s)	181, 217, 653, 999, 1403, 2380, 2957
Alw44I	GTGCAC	2 cut(s)	1638, 3314
AlwI	GGATC	7 cut(s)	1069, 1196, 1209, 1332, 2319, 3547, 3615
AlwNI	CAGNNNCTG	1 cut(s)	3995
Ama87I	CYCGRG	5 cut(s)	260, 915, 1933, 3191, 3746
Aor51HI	AGCGCT	1 cut(s)	3092
ApaI	GGGCCC	1 cut(s)	3079
ApaLI	GTGCAC	2 cut(s)	1638, 3314
ApoI	RAATTY	5 cut(s)	944, 2299, 2339, 2827, 3297
ArsI	GACNNNNNNTTYG	2 cut(s)	3586, 3618
AsiGI	ACCGGT	1 cut(s)	3318
Asp700I	GAANNNNTTC	2 cut(s)	1006, 2332
Asp718I	GGTACC	3 cut(s)	2360, 2400, 2596
AspLEI	GCGC	9 cut(s)	61, 1365, 1591, 2660, 2912, 3093, 3776, 3778, 3989
AsuC2I	CCSGG	3 cut(s)	43, 472, 3294
AsuHPI	GGTGA	8 cut(s)	154, 338, 1412, 1459, 1701, 1902, 2338, 3708
AvaI	CYCGRG	5 cut(s)	260, 915, 1933, 3191, 3746
AvaII	GGWCC	7 cut(s)	45, 142, 419, 1214, 2249, 3008, 3157
BaeGI	GKGCMC	7 cut(s)	243, 1642, 1671, 2995, 3079, 3238, 3318
BaeI	ACNNNNGTAYC	2 cut(s)	2384, 2417
BalI	TGGCCA	3 cut(s)	1624, 2812, 3046
BamHI	GGATCC	1 cut(s)	1201
BanI	GGYRCC	5 cut(s)	58, 2360, 2400, 2596, 3233
BanII	GRGCYC	2 cut(s)	198, 3079
BauI	CACGAG	1 cut(s)	3207
BbrPI	CACGTG	2 cut(s)	238, 249
BbsI	GAAGAC	6 cut(s)	869, 954, 1683, 2674, 3124, 3440
Bbv12I	GWGCWC	5 cut(s)	90, 198, 1642, 3318, 3360
BceAI	ACGGC	3 cut(s)	158, 1070, 3238
BcgI	CGANNNNNNTGC	2 cut(s)	1914, 1948
BciT130I	CCWGG	7 cut(s)	110, 616, 1356, 1626, 2451, 2605, 3107
BciVI	GTATCC	1 cut(s)	1499
BclI	TGATCA	2 cut(s)	2191, 3561
BcnI	CCSGG	3 cut(s)	43, 472, 3294
BcoDI	GTCTC	7 cut(s)	181, 217, 653, 999, 1403, 2380, 2957
BcuI	ACTAGT	1 cut(s)	4000
BfaI	CTAG	9 cut(s)	603, 1206, 1601, 1758, 2864, 2889, 3026, 3062, 4001
BfmI	CTRYAG	8 cut(s)	351, 734, 881, 1118, 1458, 1923, 2001, 2691
BfoI	RGCGCY	4 cut(s)	62, 1366, 2913, 3094
BfrI	CTTAAG	1 cut(s)	3167
BfuAI	ACCTGC	1 cut(s)	1244
BfuI	GTATCC	1 cut(s)	1499
BglI	GCCNNNNNGGC	3 cut(s)	607, 616, 3482
BglII	AGATCT	1 cut(s)	2704
BlpI	GCTNAGC	3 cut(s)	149, 1762, 3908
BmcAI	AGTACT	1 cut(s)	3678
Bme18I	GGWCC	7 cut(s)	45, 142, 419, 1214, 2249, 3008, 3157
BmeT110I	CYCGRG	5 cut(s)	260, 915, 1933, 3191, 3746
BmrI	ACTGGG	3 cut(s)	802, 1736, 3806
BmuI	ACTGGG	3 cut(s)	802, 1736, 3806
BpiI	GAAGAC	6 cut(s)	869, 954, 1683, 2674, 3124, 3440
BplI	GAGNNNNNCTC	2 cut(s)	3344, 3376
BpmI	CTGGAG	6 cut(s)	74, 139, 1734, 2514, 3115, 3648
Bpu10I	CCTNAGC	1 cut(s)	1464
Bpu1102I	GCTNAGC	3 cut(s)	149, 1762, 3908
BpuEI	CTTGAG	2 cut(s)	2071, 2576
BpuMI	CCSGG	3 cut(s)	43, 472, 3294
BsaAI	YACGTR	2 cut(s)	238, 249
BsaBI	GATNNNNATC	1 cut(s)	2287
BsaHI	GRCGYC	1 cut(s)	59
BsaI	GGTCTC	1 cut(s)	1403
BsaWI	WCCGGW	2 cut(s)	1915, 3318
BsaXI	ACNNNNNCTCC	4 cut(s)	789, 819, 3621, 3651
Bse118I	RCCGGY	2 cut(s)	3010, 3318
Bse1I	ACTGG	9 cut(s)	797, 1105, 1731, 2251, 2691, 2878, 3631, 3801, 3948
Bse3DI	GCAATG	3 cut(s)	711, 1669, 3486
Bse8I	GATNNNNATC	1 cut(s)	2287
BseBI	CCWGG	7 cut(s)	110, 616, 1356, 1626, 2451, 2605, 3107
BseGI	GGATG	6 cut(s)	554, 762, 1063, 1868, 2194, 2288
BseJI	GATNNNNATC	1 cut(s)	2287
BseMI	GCAATG	3 cut(s)	711, 1669, 3486
BseMII	CTCAG	4 cut(s)	163, 1442, 2910, 3899
BseNI	ACTGG	9 cut(s)	797, 1105, 1731, 2251, 2691, 2878, 3631, 3801, 3948
BsePI	GCGCGC	1 cut(s)	3774
BseRI	GAGGAG	3 cut(s)	122, 157, 197
BseSI	GKGCMC	7 cut(s)	243, 1642, 1671, 2995, 3079, 3238, 3318
BseYI	CCCAGC	4 cut(s)	225, 1670, 2914, 3958
Bsh1236I	CGCG	3 cut(s)	1591, 3489, 3776
BshNI	GGYRCC	5 cut(s)	58, 2360, 2400, 2596, 3233
BshTI	ACCGGT	1 cut(s)	3318
BsiHKAI	GWGCWC	5 cut(s)	90, 198, 1642, 3318, 3360
BsiHKCI	CYCGRG	5 cut(s)	260, 915, 1933, 3191, 3746
BsiSI	CCGG	6 cut(s)	42, 472, 1916, 3011, 3294, 3319
BslFI	GGGAC	9 cut(s)	92, 272, 1205, 2467, 2870, 3460, 3700, 3930, 3975
BsmAI	GTCTC	7 cut(s)	181, 217, 653, 999, 1403, 2380, 2957
BsmBI	CGTCTC	3 cut(s)	181, 217, 653
BsmFI	GGGAC	9 cut(s)	92, 272, 1205, 2467, 2870, 3460, 3700, 3930, 3975
BsmI	GAATGC	1 cut(s)	926
Bso31I	GGTCTC	1 cut(s)	1403
BsoBI	CYCGRG	5 cut(s)	260, 915, 1933, 3191, 3746
Bsp120I	GGGCCC	1 cut(s)	3075
Bsp1407I	TGTACA	1 cut(s)	2011
Bsp1720I	GCTNAGC	3 cut(s)	149, 1762, 3908
Bsp19I	CCATGG	6 cut(s)	2292, 2370, 2662, 2739, 3041, 3981
BspCNI	CTCAG	4 cut(s)	162, 1441, 2909, 3900
BspFNI	CGCG	3 cut(s)	1591, 3489, 3776
BspMAI	CTGCAG	4 cut(s)	738, 1122, 1462, 1927
BspMI	ACCTGC	1 cut(s)	1244
BspPI	GGATC	7 cut(s)	1069, 1196, 1209, 1332, 2319, 3547, 3615
BspQI	GCTCTTC	1 cut(s)	1182
BspT107I	GGYRCC	5 cut(s)	58, 2360, 2400, 2596, 3233
BspTI	CTTAAG	1 cut(s)	3167
BspTNI	GGTCTC	1 cut(s)	1403
BsrBI	CCGCTC	1 cut(s)	130
BsrDI	GCAATG	3 cut(s)	711, 1669, 3486
BsrFI	RCCGGY	2 cut(s)	3010, 3318
BsrGI	TGTACA	1 cut(s)	2011
BsrI	ACTGG	9 cut(s)	797, 1105, 1731, 2251, 2691, 2878, 3631, 3801, 3948
BssAI	RCCGGY	2 cut(s)	3010, 3318
BssHII	GCGCGC	1 cut(s)	3774
BssNI	GRCGYC	1 cut(s)	59
BssSI	CACGAG	1 cut(s)	3207
BssT1I	CCWWGG	8 cut(s)	321, 2292, 2370, 2662, 2739, 3041, 3241, 3981
Bst2BI	CACGAG	1 cut(s)	3207
Bst2UI	CCWGG	7 cut(s)	110, 616, 1356, 1626, 2451, 2605, 3107
Bst4CI	ACNGT	8 cut(s)	122, 1495, 2011, 2233, 2279, 3130, 3146, 3927
Bst6I	CTCTTC	6 cut(s)	1182, 1284, 1806, 2323, 3345, 3369
BstACI	GRCGYC	1 cut(s)	59
BstAFI	CTTAAG	1 cut(s)	3167
BstAPI	GCANNNNNTGC	3 cut(s)	701, 1130, 3671
BstAUI	TGTACA	1 cut(s)	2011
BstBAI	YACGTR	2 cut(s)	238, 249
BstDEI	CTNAG	9 cut(s)	149, 559, 766, 1428, 1464, 1762, 2896, 3908, 3920
BstDSI	CCRYGG	7 cut(s)	2292, 2370, 2662, 2739, 3041, 3598, 3981
BstEII	GGTNACC	1 cut(s)	1707
BstENI	CCTNNNNNAGG	2 cut(s)	263, 1209
BstF5I	GGATG	6 cut(s)	554, 762, 1063, 1868, 2194, 2288
BstFNI	CGCG	3 cut(s)	1591, 3489, 3776
BstH2I	RGCGCY	4 cut(s)	62, 1366, 2913, 3094
BstHHI	GCGC	9 cut(s)	61, 1365, 1591, 2660, 2912, 3093, 3776, 3778, 3989
BstMAI	GTCTC	7 cut(s)	181, 217, 653, 999, 1403, 2380, 2957
BstNI	CCWGG	7 cut(s)	110, 616, 1356, 1626, 2451, 2605, 3107
BstNSI	RCATGY	4 cut(s)	1249, 1537, 2658, 3055
BstPI	GGTNACC	1 cut(s)	1707
BstSFI	CTRYAG	8 cut(s)	351, 734, 881, 1118, 1458, 1923, 2001, 2691
BstSLI	GKGCMC	7 cut(s)	243, 1642, 1671, 2995, 3079, 3238, 3318
BstUI	CGCG	3 cut(s)	1591, 3489, 3776
BstV2I	GAAGAC	6 cut(s)	869, 954, 1683, 2674, 3124, 3440
BstX2I	RGATCY	2 cut(s)	1201, 2704
BstXI	CCANNNNNNTGG	3 cut(s)	792, 2371, 3269
BstYI	RGATCY	2 cut(s)	1201, 2704
BsuI	GTATCC	1 cut(s)	1499
BtgI	CCRYGG	7 cut(s)	2292, 2370, 2662, 2739, 3041, 3598, 3981
BtsCI	GGATG	6 cut(s)	554, 762, 1063, 1868, 2194, 2288
BtsI	GCAGTG	2 cut(s)	2151, 3232
BveI	ACCTGC	1 cut(s)	1244
CaiI	CAGNNNCTG	1 cut(s)	3995
CfoI	GCGC	9 cut(s)	61, 1365, 1591, 2660, 2912, 3093, 3776, 3778, 3989
Cfr10I	RCCGGY	2 cut(s)	3010, 3318
CseI	GACGC	2 cut(s)	1273, 3478
Csp6I	GTAC	8 cut(s)	50, 763, 2012, 2361, 2401, 2447, 2597, 3677
CspAI	ACCGGT	1 cut(s)	3318
CspCI	CAANNNNNGTGG	4 cut(s)	307, 342, 2860, 2895
CviQI	GTAC	8 cut(s)	50, 763, 2012, 2361, 2401, 2447, 2597, 3677
DdeI	CTNAG	9 cut(s)	149, 559, 766, 1428, 1464, 1762, 2896, 3908, 3920
DinI	GGCGCC	1 cut(s)	60
DraIII	CACNNNGTG	1 cut(s)	2146
DrdI	GACNNNNNNGTC	1 cut(s)	426
DseDI	GACNNNNNNGTC	1 cut(s)	426
EaeI	YGGCCR	6 cut(s)	39, 828, 1622, 2810, 3012, 3044
Eam1104I	CTCTTC	6 cut(s)	1182, 1284, 1806, 2323, 3345, 3369
EarI	CTCTTC	6 cut(s)	1182, 1284, 1806, 2323, 3345, 3369
EciI	GGCGGA	3 cut(s)	94, 160, 3552
Ecl136II	GAGCTC	1 cut(s)	196
Eco130I	CCWWGG	8 cut(s)	321, 2292, 2370, 2662, 2739, 3041, 3241, 3981
Eco147I	AGGCCT	1 cut(s)	1488
Eco24I	GRGCYC	2 cut(s)	198, 3079
Eco31I	GGTCTC	1 cut(s)	1403
Eco32I	GATATC	3 cut(s)	1821, 2611, 3531
Eco47I	GGWCC	7 cut(s)	45, 142, 419, 1214, 2249, 3008, 3157
Eco47III	AGCGCT	1 cut(s)	3092
Eco53kI	GAGCTC	1 cut(s)	196
Eco57I	CTGAAG	2 cut(s)	2340, 2781
Eco72I	CACGTG	2 cut(s)	238, 249
Eco88I	CYCGRG	5 cut(s)	260, 915, 1933, 3191, 3746
Eco91I	GGTNACC	1 cut(s)	1707
EcoICRI	GAGCTC	1 cut(s)	196
EcoNI	CCTNNNNNAGG	2 cut(s)	263, 1209
EcoO109I	RGGNCCY	1 cut(s)	1214
EcoO65I	GGTNACC	1 cut(s)	1707
EcoRI	GAATTC	1 cut(s)	3297
EcoRII	CCWGG	7 cut(s)	108, 614, 1354, 1624, 2449, 2603, 3105
EcoRV	GATATC	3 cut(s)	1821, 2611, 3531
EcoT14I	CCWWGG	8 cut(s)	321, 2292, 2370, 2662, 2739, 3041, 3241, 3981
EcoT22I	ATGCAT	4 cut(s)	556, 1153, 1247, 3053
EcoT38I	GRGCYC	2 cut(s)	198, 3079
EgeI	GGCGCC	1 cut(s)	60
EheI	GGCGCC	1 cut(s)	60
ErhI	CCWWGG	8 cut(s)	321, 2292, 2370, 2662, 2739, 3041, 3241, 3981
Esp3I	CGTCTC	3 cut(s)	181, 217, 653
FalI	AAGNNNNNCTT	2 cut(s)	3161, 3193
FaqI	GGGAC	9 cut(s)	92, 272, 1205, 2467, 2870, 3460, 3700, 3930, 3975
FauI	CCCGC	3 cut(s)	135, 3392, 3813
FauNDI	CATATG	2 cut(s)	536, 3826
FbaI	TGATCA	2 cut(s)	2191, 3561
FblI	GTMKAC	1 cut(s)	904
FokI	GGATG	6 cut(s)	541, 769, 1070, 1875, 2181, 2275
FriOI	GRGCYC	2 cut(s)	198, 3079
FspBI	CTAG	9 cut(s)	603, 1206, 1601, 1758, 2864, 2889, 3026, 3062, 4001
GlaI	GCGC	9 cut(s)	60, 1364, 1590, 2659, 2911, 3092, 3775, 3777, 3988
GsaI	CCCAGC	4 cut(s)	229, 1674, 2918, 3962
GsuI	CTGGAG	6 cut(s)	74, 139, 1734, 2514, 3115, 3648
HaeII	RGCGCY	4 cut(s)	62, 1366, 2913, 3094
HapII	CCGG	6 cut(s)	42, 472, 1916, 3011, 3294, 3319
HgaI	GACGC	2 cut(s)	1273, 3478
HhaI	GCGC	9 cut(s)	61, 1365, 1591, 2660, 2912, 3093, 3776, 3778, 3989
Hin1I	GRCGYC	1 cut(s)	59
Hin6I	GCGC	9 cut(s)	59, 1363, 1589, 2658, 2910, 3091, 3774, 3776, 3987
HinP1I	GCGC	9 cut(s)	59, 1363, 1589, 2658, 2910, 3091, 3774, 3776, 3987
HincII	GTYRAC	3 cut(s)	905, 1419, 2526
HindII	GTYRAC	3 cut(s)	905, 1419, 2526
HindIII	AAGCTT	1 cut(s)	1578
HpaII	CCGG	6 cut(s)	42, 472, 1916, 3011, 3294, 3319
HphI	GGTGA	8 cut(s)	154, 338, 1412, 1459, 1701, 1902, 2338, 3708
Hpy166II	GTNNAC	7 cut(s)	905, 1419, 1528, 1640, 2526, 2684, 3316
Hpy8I	GTNNAC	7 cut(s)	905, 1419, 1528, 1640, 2526, 2684, 3316
Hpy99I	CGWCG	7 cut(s)	25, 37, 194, 777, 906, 1091, 1267
HpyCH4III	ACNGT	8 cut(s)	122, 1495, 2011, 2233, 2279, 3130, 3146, 3927
HpyCH4IV	ACGT	6 cut(s)	237, 248, 275, 2537, 2989, 3689
HpyF3I	CTNAG	9 cut(s)	149, 559, 766, 1428, 1464, 1762, 2896, 3908, 3920
HpySE526I	ACGT	6 cut(s)	237, 248, 275, 2537, 2989, 3689
Hsp92I	GRCGYC	1 cut(s)	59
HspAI	GCGC	9 cut(s)	59, 1363, 1589, 2658, 2910, 3091, 3774, 3776, 3987
KasI	GGCGCC	1 cut(s)	58
KpnI	GGTACC	3 cut(s)	2364, 2404, 2600
Ksp22I	TGATCA	2 cut(s)	2191, 3561
LguI	GCTCTTC	1 cut(s)	1182
LmnI	GCTCC	5 cut(s)	93, 135, 158, 1357, 2907
MaeI	CTAG	9 cut(s)	603, 1206, 1601, 1758, 2864, 2889, 3026, 3062, 4001
MaeII	ACGT	6 cut(s)	237, 248, 275, 2537, 2989, 3689
MaeIII	GTNAC	8 cut(s)	122, 543, 745, 1273, 1707, 1908, 2764, 3098
MbiI	CCGCTC	1 cut(s)	130
MfeI	CAATTG	1 cut(s)	410
MflI	RGATCY	2 cut(s)	1201, 2704
MlsI	TGGCCA	3 cut(s)	1624, 2812, 3046
MluI	ACGCGT	1 cut(s)	3487
MluNI	TGGCCA	3 cut(s)	1624, 2812, 3046
Mly113I	GGCGCC	1 cut(s)	59
MmeI	TCCRAC	1 cut(s)	44
Mox20I	TGGCCA	3 cut(s)	1624, 2812, 3046
Mph1103I	ATGCAT	4 cut(s)	556, 1153, 1247, 3053
MroXI	GAANNNNTTC	2 cut(s)	1006, 2332
MscI	TGGCCA	3 cut(s)	1624, 2812, 3046
MseI	TTAA	7 cut(s)	381, 654, 974, 1128, 1974, 3168, 3197
MslI	CAYNNNNRTG	4 cut(s)	722, 2369, 3046, 3144
Msp20I	TGGCCA	3 cut(s)	1624, 2812, 3046
MspA1I	CMGCKG	7 cut(s)	73, 94, 886, 1073, 2054, 2759, 3995
MspCI	CTTAAG	1 cut(s)	3167
MspI	CCGG	6 cut(s)	42, 472, 1916, 3011, 3294, 3319
MunI	CAATTG	1 cut(s)	410
Mva1269I	GAATGC	1 cut(s)	926
MvaI	CCWGG	7 cut(s)	110, 616, 1356, 1626, 2451, 2605, 3107
MvnI	CGCG	3 cut(s)	1591, 3489, 3776
NarI	GGCGCC	1 cut(s)	59
NciI	CCSGG	3 cut(s)	43, 472, 3294
NcoI	CCATGG	6 cut(s)	2292, 2370, 2662, 2739, 3041, 3981
NdeI	CATATG	2 cut(s)	536, 3826
NmeAIII	GCCGAG	1 cut(s)	90
NmuCI	GTSAC	4 cut(s)	122, 1707, 1908, 3098
NsiI	ATGCAT	4 cut(s)	556, 1153, 1247, 3053
NspI	RCATGY	4 cut(s)	1249, 1537, 2658, 3055
OliI	CACNNNNGTG	1 cut(s)	3144
PaeI	GCATGC	2 cut(s)	2658, 3055
PaeR7I	CTCGAG	1 cut(s)	260
PauI	GCGCGC	1 cut(s)	3774
PceI	AGGCCT	1 cut(s)	1488
PciSI	GCTCTTC	1 cut(s)	1182
PcsI	WCGNNNNNNNCGW	1 cut(s)	29
PctI	GAATGC	1 cut(s)	926
PdmI	GAANNNNTTC	2 cut(s)	1006, 2332
PfeI	GAWTC	5 cut(s)	1091, 1450, 1514, 2238, 3707
PflFI	GACNNNGTC	1 cut(s)	100
PflMI	CCANNNNNTGG	2 cut(s)	249, 1140
PfoI	TCCNGGA	1 cut(s)	1354
PinAI	ACCGGT	1 cut(s)	3318
PluTI	GGCGCC	1 cut(s)	62
PmaCI	CACGTG	2 cut(s)	238, 249
PmlI	CACGTG	2 cut(s)	238, 249
Ppu21I	YACGTR	2 cut(s)	238, 249
PpuMI	RGGWCCY	1 cut(s)	1214
PsiI	TTATAA	3 cut(s)	440, 1898, 2967
Psp124BI	GAGCTC	1 cut(s)	198
Psp5II	RGGWCCY	1 cut(s)	1214
Psp6I	CCWGG	7 cut(s)	108, 614, 1354, 1624, 2449, 2603, 3105
PspCI	CACGTG	2 cut(s)	238, 249
PspEI	GGTNACC	1 cut(s)	1707
PspFI	CCCAGC	4 cut(s)	225, 1670, 2914, 3958
PspGI	CCWGG	7 cut(s)	108, 614, 1354, 1624, 2449, 2603, 3105
PspOMI	GGGCCC	1 cut(s)	3075
PspPPI	RGGWCCY	1 cut(s)	1214
PstI	CTGCAG	4 cut(s)	738, 1122, 1462, 1927
PstNI	CAGNNNCTG	1 cut(s)	3995
PsuI	RGATCY	2 cut(s)	1201, 2704
PsyI	GACNNNGTC	1 cut(s)	100
PteI	GCGCGC	1 cut(s)	3774
PvuII	CAGCTG	7 cut(s)	73, 94, 886, 1073, 2054, 2759, 3995
RsaI	GTAC	8 cut(s)	51, 764, 2013, 2362, 2402, 2448, 2598, 3678
RsaNI	GTAC	8 cut(s)	50, 763, 2012, 2361, 2401, 2447, 2597, 3677
RseI	CAYNNNNRTG	4 cut(s)	722, 2369, 3046, 3144
SacI	GAGCTC	1 cut(s)	198
SalI	GTCGAC	1 cut(s)	903
SapI	GCTCTTC	1 cut(s)	1182
SaqAI	TTAA	7 cut(s)	381, 654, 974, 1128, 1974, 3168, 3197
ScaI	AGTACT	1 cut(s)	3678
SfcI	CTRYAG	8 cut(s)	351, 734, 881, 1118, 1458, 1923, 2001, 2691
SfiI	GGCCNNNNNGGCC	1 cut(s)	616
SfoI	GGCGCC	1 cut(s)	60
Sfr274I	CTCGAG	1 cut(s)	260
SinI	GGWCC	7 cut(s)	45, 142, 419, 1214, 2249, 3008, 3157
SlaI	CTCGAG	1 cut(s)	260
SmiMI	CAYNNNNRTG	4 cut(s)	722, 2369, 3046, 3144
SmlI	CTYRAG	4 cut(s)	260, 2086, 2591, 3167
SmoI	CTYRAG	4 cut(s)	260, 2086, 2591, 3167
SpeI	ACTAGT	1 cut(s)	4000
SphI	GCATGC	2 cut(s)	2658, 3055
SseBI	AGGCCT	1 cut(s)	1488
SspDI	GGCGCC	1 cut(s)	58
SspI	AATATT	2 cut(s)	1282, 3897
SspMI	CTAG	9 cut(s)	603, 1206, 1601, 1758, 2864, 2889, 3026, 3062, 4001
SstI	GAGCTC	1 cut(s)	198
StuI	AGGCCT	1 cut(s)	1488
StyI	CCWWGG	8 cut(s)	321, 2292, 2370, 2662, 2739, 3041, 3241, 3981
TaaI	ACNGT	8 cut(s)	122, 1495, 2011, 2233, 2279, 3130, 3146, 3927
TaiI	ACGT	6 cut(s)	240, 251, 278, 2540, 2992, 3692
TatI	WGTACW	3 cut(s)	762, 2011, 3676
TauI	GCSGC	8 cut(s)	64, 830, 1368, 1564, 2825, 3479, 3733, 3754
TfiI	GAWTC	5 cut(s)	1091, 1450, 1514, 2238, 3707
Tru1I	TTAA	7 cut(s)	381, 654, 974, 1128, 1974, 3168, 3197
Tru9I	TTAA	7 cut(s)	381, 654, 974, 1128, 1974, 3168, 3197
TseFI	GTSAC	4 cut(s)	122, 1707, 1908, 3098
Tsp45I	GTSAC	4 cut(s)	122, 1707, 1908, 3098
TspGWI	ACGGA	5 cut(s)	37, 178, 1014, 1331, 2814
Tth111I	GACNNNGTC	1 cut(s)	100
Van91I	CCANNNNNTGG	2 cut(s)	249, 1140
Vha464I	CTTAAG	1 cut(s)	3167
VneI	GTGCAC	2 cut(s)	1638, 3314
VpaK11BI	GGWCC	7 cut(s)	45, 142, 419, 1214, 2249, 3008, 3157
XagI	CCTNNNNNAGG	2 cut(s)	263, 1209
XapI	RAATTY	5 cut(s)	944, 2299, 2339, 2827, 3297
XceI	RCATGY	4 cut(s)	1249, 1537, 2658, 3055
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3269
XhoI	CTCGAG	1 cut(s)	260
XmiI	GTMKAC	1 cut(s)	904
XmnI	GAANNNNTTC	2 cut(s)	1006, 2332
XspI	CTAG	9 cut(s)	603, 1206, 1601, 1758, 2864, 2889, 3026, 3062, 4001
ZrmI	AGTACT	1 cut(s)	3678
Zsp2I	ATGCAT	4 cut(s)	556, 1153, 1247, 3053

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.