MD03G1272900.v1.1

phosphotransfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007004
Physical Location & Seq
Forward (+)
35623827 .. 35626300
2474 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1272900.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGACGCCGTGACTCAGTGGCGGAAGCAGTGGTTCGACTACACTCAGTATCTGCGCCGAGAGGGATTCTTGGACGATCAGTTTGCACAGCTAAAGAAGCTGCAGGATGAAAACAGCCCAGATTTTGTAGTTGAAGTTGTTTCTCTTTTCTTTCAAGATTCTGAGAAGCTTTTCAACAATATGGCCAGGGCTCTAGAACAGAACGTTGTAAACTTCAAACAGGTAGATGCCTATGTCCATCAATTCAAGGGTAGCAGTGCCTGGATAGGTGCATTCAGACTTAAAAATGTATGCATCAACTTCAGAAATTTTTGTGAGGCTCAGAACCTTGAAGGGTGTTTGAGATGTTTGCAACAACTGCAGCAAGAGAGCTCTGCACTTAAGAACAACCTTGAAAATTTATTTAGGTTGGAGCAACAGATCGTGGCTGCTGGCGGGTCAATTCCGATGATGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Hpt

Protein Analysis

153

Amino Acids

17.79

Weight (kDa)

5.26

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hpt PF01627 45 - 129 1.3e-14 Hpt domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 22, 435
AclI AACGTT 1 cut(s) 204
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 2 cut(s) 307, 397
AcuI CTGAAG 1 cut(s) 286
AcyI GRCGYC 1 cut(s) 6
AflII CTTAAG 1 cut(s) 380
AgsI TTSAA 7 cut(s) 134, 155, 175, 217, 247, 332, 395
AjnI CCWGG 2 cut(s) 185, 260
AluBI AGCT 4 cut(s) 91, 100, 169, 372
AluI AGCT 4 cut(s) 91, 100, 169, 372
Alw21I GWGCWC 1 cut(s) 374
AlwNI CAGNNNCTG 2 cut(s) 52, 261
AoxI GGCC 1 cut(s) 183
ApeKI GCWGC 3 cut(s) 100, 361, 428
ApoI RAATTY 2 cut(s) 307, 397
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
Asp700I GAANNNNTTC 1 cut(s) 170
AspLEI GCGC 1 cut(s) 57
BalI TGGCCA 1 cut(s) 185
BanII GRGCYC 2 cut(s) 193, 374
Bbv12I GWGCWC 1 cut(s) 374
BbvI GCAGC 3 cut(s) 87, 373, 415
BccI CCATC 2 cut(s) 246, 445
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 2 cut(s) 187, 262
BfaI CTAG 1 cut(s) 194
BfmI CTRYAG 2 cut(s) 101, 359
BfrI CTTAAG 1 cut(s) 380
BisI GCNGC 3 cut(s) 101, 362, 429
BlsI GCNGC 3 cut(s) 102, 363, 430
Bme1390I CCNGG 2 cut(s) 187, 262
BmrFI CCNGG 2 cut(s) 187, 262
BmsI GCATC 2 cut(s) 217, 303
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 1 cut(s) 186
BseBI CCWGG 2 cut(s) 187, 262
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 112
BseMII CTCAG 4 cut(s) 29, 59, 153, 335
BseXI GCAGC 3 cut(s) 87, 373, 415
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 374
BsmI GAATGC 1 cut(s) 272
BsnI GGCC 1 cut(s) 185
Bsp1286I GDGCHC 2 cut(s) 193, 374
Bsp143I GATC 2 cut(s) 76, 420
BspACI CCGC 2 cut(s) 22, 435
BspANI GGCC 1 cut(s) 185
BspCNI CTCAG 4 cut(s) 28, 58, 154, 334
BspMAI CTGCAG 2 cut(s) 105, 363
BspTI CTTAAG 1 cut(s) 380
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 2 cut(s) 76, 420
BssNI GRCGYC 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 187, 262
BstACI GRCGYC 1 cut(s) 6
BstAFI CTTAAG 1 cut(s) 380
BstAPI GCANNNNNTGC 1 cut(s) 358
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 4 cut(s) 15, 45, 162, 321
BstF5I GGATG 1 cut(s) 112
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 2 cut(s) 79, 423
BstMBI GATC 2 cut(s) 76, 420
BstMWI GCNNNNNNNGC 2 cut(s) 97, 358
BstNI CCWGG 2 cut(s) 187, 262
BstSCI CCNGG 2 cut(s) 185, 260
BstSFI CTRYAG 2 cut(s) 101, 359
BstV1I GCAGC 3 cut(s) 87, 373, 415
BsuRI GGCC 1 cut(s) 185
BtsCI GGATG 1 cut(s) 112
BtsI GCAGTG 2 cut(s) 35, 262
BtsIMutI CAGTG 3 cut(s) 23, 35, 262
Cac8I GCNNGC 1 cut(s) 433
CaiI CAGNNNCTG 2 cut(s) 52, 261
CfoI GCGC 1 cut(s) 57
CseI GACGC 1 cut(s) 14
CviJI RGCY 9 cut(s) 91, 100, 117, 169, 185, 191, 320, 372, 428
CviKI_1 RGCY 9 cut(s) 91, 100, 117, 169, 185, 191, 320, 372, 428
DdeI CTNAG 4 cut(s) 15, 45, 162, 321
DpnI GATC 2 cut(s) 78, 422
DpnII GATC 2 cut(s) 76, 420
EaeI YGGCCR 1 cut(s) 183
EciI GGCGGA 1 cut(s) 37
Ecl136II GAGCTC 1 cut(s) 372
Eco24I GRGCYC 2 cut(s) 193, 374
Eco53kI GAGCTC 1 cut(s) 372
Eco57I CTGAAG 1 cut(s) 286
EcoICRI GAGCTC 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 185, 260
EcoT22I ATGCAT 1 cut(s) 296
EcoT38I GRGCYC 2 cut(s) 193, 374
FaiI YATR 3 cut(s) 182, 234, 292
FauI CCCGC 1 cut(s) 428
Fnu4HI GCNGC 3 cut(s) 101, 362, 429
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 2 cut(s) 193, 374
Fsp4HI GCNGC 3 cut(s) 101, 362, 429
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 56
GluI GCNGC 3 cut(s) 101, 362, 429
HaeIII GGCC 1 cut(s) 185
HgaI GACGC 1 cut(s) 14
HhaI GCGC 1 cut(s) 57
Hin1I GRCGYC 1 cut(s) 6
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 167
HinfI GANTC 3 cut(s) 13, 66, 158
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 5 cut(s) 163, 278, 305, 324, 447
Hpy188III TCNNGA 2 cut(s) 155, 194
Hpy8I GTNNAC 1 cut(s) 211
HpyAV CCTTC 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 7 cut(s) 86, 103, 272, 294, 352, 361, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 358
HpyF3I CTNAG 4 cut(s) 15, 45, 162, 321
HpySE526I ACGT 1 cut(s) 204
Hsp92I GRCGYC 1 cut(s) 6
HspAI GCGC 1 cut(s) 55
Kzo9I GATC 2 cut(s) 76, 420
LmnI GCTCC 1 cut(s) 412
LpnPI CCDG 8 cut(s) 89, 132, 172, 199, 206, 247, 274, 417
Lsp1109I GCAGC 3 cut(s) 87, 373, 415
LweI GCATC 2 cut(s) 217, 303
MaeI CTAG 1 cut(s) 194
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 10
MalI GATC 2 cut(s) 78, 422
MboI GATC 2 cut(s) 76, 420
MhlI GDGCHC 2 cut(s) 193, 374
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 4 cut(s) 242, 307, 397, 441
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 2 cut(s) 55, 310
Mox20I TGGCCA 1 cut(s) 185
Mph1103I ATGCAT 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 170
MscI TGGCCA 1 cut(s) 185
MseI TTAA 2 cut(s) 282, 381
Msp20I TGGCCA 1 cut(s) 185
MspCI CTTAAG 1 cut(s) 380
MspR9I CCNGG 2 cut(s) 187, 262
Mva1269I GAATGC 1 cut(s) 272
MvaI CCWGG 2 cut(s) 187, 262
MwoI GCNNNNNNNGC 2 cut(s) 97, 358
NdeII GATC 2 cut(s) 76, 420
NmeAIII GCCGAG 1 cut(s) 83
NmuCI GTSAC 1 cut(s) 10
NsiI ATGCAT 1 cut(s) 296
PctI GAATGC 1 cut(s) 272
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 2 cut(s) 66, 158
PkrI GCNGC 3 cut(s) 102, 363, 430
PleI GAGTC 1 cut(s) 7
PpsI GAGTC 1 cut(s) 7
Psp124BI GAGCTC 1 cut(s) 374
Psp1406I AACGTT 1 cut(s) 204
Psp6I CCWGG 2 cut(s) 185, 260
PspGI CCWGG 2 cut(s) 185, 260
PstI CTGCAG 2 cut(s) 105, 363
PstNI CAGNNNCTG 2 cut(s) 52, 261
SacI GAGCTC 1 cut(s) 374
SaqAI TTAA 2 cut(s) 282, 381
SatI GCNGC 3 cut(s) 101, 362, 429
Sau3AI GATC 2 cut(s) 76, 420
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 187, 262
SduI GDGCHC 2 cut(s) 193, 374
SfaNI GCATC 2 cut(s) 217, 303
SfcI CTRYAG 2 cut(s) 101, 359
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 4 cut(s) 242, 307, 397, 441
SsiI CCGC 2 cut(s) 22, 435
SspMI CTAG 1 cut(s) 194
SstI GAGCTC 1 cut(s) 374
StyD4I CCNGG 2 cut(s) 185, 260
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 36
TasI AATT 4 cut(s) 242, 307, 397, 441
TfiI GAWTC 2 cut(s) 66, 158
Tru1I TTAA 2 cut(s) 282, 381
Tru9I TTAA 2 cut(s) 282, 381
TscAI CASTG 3 cut(s) 23, 35, 262
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 3 cut(s) 100, 361, 428
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 1 cut(s) 123
TspRI CASTG 3 cut(s) 23, 35, 262
Vha464I CTTAAG 1 cut(s) 380
XapI RAATTY 2 cut(s) 307, 397
XbaI TCTAGA 1 cut(s) 193
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 1 cut(s) 194
Zsp2I ATGCAT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.