Rroxscaffold_7G00175370

phosphotransfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007004
Physical Location & Seq
Reverse (-)
14936508 .. 14938718
2211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00175370.1

Sequence Viewer

Length: 459 bp
ATGGATGCTGTGGCTCAGTGGCGGAACCAGTGGTTTGACTACACCCATTATCTGCGACGAGAGGGATTCTTGGATGATCAGTTTGCACAACTGAAGAAGCTCCAGGATGAAAACAGCCCAGATTTCGTGGTTGAGGTTGTTTCTCTGTTCTTTCAAGATTCTGAGAAGCTTCTCAACAATATGGCCAGGGCTCTAGAGCAGAATGTTGTAGATTTCAAACAAGTAGATGCCTGTGTCCATCAATTCAAGGGTAGCAGTGCCTGGATAGGTGCAATGAGACTTAAAAATGTATGCATCAACTTCAGAAATTATTGTGAGGCGCAGAACCTTGAGGGGTGTTTGGGGTGTTTGGAACGAGTACGACAAGAATGCTTGGCATTAAAGAACAATCTTGAAAACTTGTTTAGGTTGGAGCAACAGATAGTGGCTGCTGGTGGAGCAATTCCTGTTATGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Hpt

Protein Analysis

152

Amino Acids

17.6

Weight (kDa)

5.1

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hpt PF01627 45 - 129 2.9e-16 Hpt domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 22
AcoI YGGCCR 1 cut(s) 183
AcuI CTGAAG 2 cut(s) 113, 286
AfaI GTAC 1 cut(s) 360
AgsI TTSAA 4 cut(s) 155, 217, 247, 395
AjnI CCWGG 3 cut(s) 102, 185, 260
AluBI AGCT 2 cut(s) 100, 169
AluI AGCT 2 cut(s) 100, 169
Alw26I GTCTC 1 cut(s) 271
AlwNI CAGNNNCTG 1 cut(s) 261
AoxI GGCC 1 cut(s) 183
ApeKI GCWGC 1 cut(s) 428
AspLEI GCGC 1 cut(s) 322
BalI TGGCCA 1 cut(s) 185
BanII GRGCYC 1 cut(s) 193
BbvI GCAGC 1 cut(s) 415
BccI CCATC 1 cut(s) 246
BcgI CGANNNNNNTGC 2 cut(s) 351, 385
BciT130I CCWGG 3 cut(s) 104, 187, 262
BclI TGATCA 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 271
BfaI CTAG 1 cut(s) 194
BisI GCNGC 1 cut(s) 429
BlsI GCNGC 1 cut(s) 430
Bme1390I CCNGG 3 cut(s) 104, 187, 262
BmiI GGNNCC 1 cut(s) 26
BmrFI CCNGG 3 cut(s) 104, 187, 262
BmsI GCATC 2 cut(s) 217, 303
BpmI CTGGAG 1 cut(s) 86
BpuEI CTTGAG 1 cut(s) 350
BsaJI CCNNGG 1 cut(s) 186
Bse1I ACTGG 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 279
BseBI CCWGG 3 cut(s) 104, 187, 262
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 3 cut(s) 10, 79, 112
BseMI GCAATG 1 cut(s) 279
BseMII CTCAG 2 cut(s) 29, 153
BseNI ACTGG 1 cut(s) 28
BseXI GCAGC 1 cut(s) 415
BshFI GGCC 1 cut(s) 185
BsmAI GTCTC 1 cut(s) 271
BsmI GAATGC 1 cut(s) 374
BsnI GGCC 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 1 cut(s) 76
BspACI CCGC 1 cut(s) 22
BspANI GGCC 1 cut(s) 185
BspCNI CTCAG 2 cut(s) 28, 154
BspLI GGNNCC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 279
BsrI ACTGG 1 cut(s) 28
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 1 cut(s) 76
Bst2UI CCWGG 3 cut(s) 104, 187, 262
BstDEI CTNAG 2 cut(s) 15, 162
BstF5I GGATG 3 cut(s) 10, 79, 112
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 1 cut(s) 79
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 1 cut(s) 76
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 3 cut(s) 104, 187, 262
BstSCI CCNGG 3 cut(s) 102, 185, 260
BstV1I GCAGC 1 cut(s) 415
BsuRI GGCC 1 cut(s) 185
BtsCI GGATG 3 cut(s) 10, 79, 112
BtsI GCAGTG 1 cut(s) 262
BtsIMutI CAGTG 3 cut(s) 23, 35, 262
CaiI CAGNNNCTG 1 cut(s) 261
CfoI GCGC 1 cut(s) 322
Csp6I GTAC 1 cut(s) 359
CviJI RGCY 7 cut(s) 14, 100, 117, 169, 185, 191, 428
CviKI_1 RGCY 7 cut(s) 14, 100, 117, 169, 185, 191, 428
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 2 cut(s) 15, 162
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 183
EciI GGCGGA 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 193
Eco57I CTGAAG 2 cut(s) 113, 286
EcoRII CCWGG 3 cut(s) 102, 185, 260
EcoT22I ATGCAT 1 cut(s) 296
EcoT38I GRGCYC 1 cut(s) 193
FaiI YATR 3 cut(s) 182, 292, 452
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 1 cut(s) 429
FokI GGATG 3 cut(s) 17, 86, 119
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 1 cut(s) 429
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 321
GluI GCNGC 1 cut(s) 429
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 1 cut(s) 185
HhaI GCGC 1 cut(s) 322
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HindIII AAGCTT 1 cut(s) 167
HinfI GANTC 2 cut(s) 66, 158
Hpy188I TCNGA 2 cut(s) 163, 305
Hpy188III TCNNGA 3 cut(s) 155, 194, 392
Hpy99I CGWCG 1 cut(s) 60
HpyCH4V TGCA 3 cut(s) 86, 272, 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 2 cut(s) 15, 162
HspAI GCGC 1 cut(s) 320
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 1 cut(s) 76
LmnI GCTCC 3 cut(s) 105, 412, 437
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 2 cut(s) 217, 303
MaeI CTAG 1 cut(s) 194
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MboII GAAGA 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 193
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 3 cut(s) 242, 307, 441
MluNI TGGCCA 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 4 cut(s) 55, 127, 310, 325
Mox20I TGGCCA 1 cut(s) 185
Mph1103I ATGCAT 1 cut(s) 296
MscI TGGCCA 1 cut(s) 185
MseI TTAA 2 cut(s) 282, 380
Msp20I TGGCCA 1 cut(s) 185
MspR9I CCNGG 3 cut(s) 104, 187, 262
Mva1269I GAATGC 1 cut(s) 374
MvaI CCWGG 3 cut(s) 104, 187, 262
MwoI GCNNNNNNNGC 1 cut(s) 437
NdeII GATC 1 cut(s) 76
NlaIV GGNNCC 1 cut(s) 26
NsiI ATGCAT 1 cut(s) 296
PctI GAATGC 1 cut(s) 374
PfeI GAWTC 2 cut(s) 66, 158
PfoI TCCNGGA 1 cut(s) 102
PkrI GCNGC 1 cut(s) 430
Psp6I CCWGG 3 cut(s) 102, 185, 260
PspGI CCWGG 3 cut(s) 102, 185, 260
PspN4I GGNNCC 1 cut(s) 26
PstNI CAGNNNCTG 1 cut(s) 261
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
SaqAI TTAA 2 cut(s) 282, 380
SatI GCNGC 1 cut(s) 429
Sau3AI GATC 1 cut(s) 76
ScrFI CCNGG 3 cut(s) 104, 187, 262
SduI GDGCHC 1 cut(s) 193
SetI ASST 6 cut(s) 102, 138, 171, 271, 330, 410
SfaNI GCATC 2 cut(s) 217, 303
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 3 cut(s) 242, 307, 441
SsiI CCGC 1 cut(s) 22
SspMI CTAG 1 cut(s) 194
StyD4I CCNGG 3 cut(s) 102, 185, 260
TasI AATT 3 cut(s) 242, 307, 441
TfiI GAWTC 2 cut(s) 66, 158
Tru1I TTAA 2 cut(s) 282, 380
Tru9I TTAA 2 cut(s) 282, 380
TscAI CASTG 3 cut(s) 23, 35, 262
TseI GCWGC 1 cut(s) 428
TspDTI ATGAA 1 cut(s) 123
TspRI CASTG 3 cut(s) 23, 35, 262
XbaI TCTAGA 1 cut(s) 193
XspI CTAG 1 cut(s) 194
Zsp2I ATGCAT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.