MD11G1293900.v1.1

phosphotransfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007004
Physical Location & Seq
Forward (+)
41305280 .. 41307736
2457 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1293900.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGACGCCGTGACTCAGTGGCAGAAGCAGTGGTTCGACTACACTCAGTATCTGCGCCGAGAGGGATTCTTGGACGATCAATTTGCTCAGCTAAAGAAGCTGCAGGATGAAAACAGCCCAGATTTTGTAGTTGAAGTTGTTTCTCTTTTCTTTCAAGATACTGAGAAGCTTCTCAACAATATGGCCAGGGCTCTAGAACAGAACGTTGTAAATTTCGAACAGGTAGATGTCTATGTTCATCAATTCAAGGGTAGCAGTGCCTGGATAGGTGCATTGAGACTTAAAAATGCATGCATCAACTTCAGAAATTTTTGTGAGGCCCGGAACCTTGAAGGGTGTTTGAGATGTTTGCAACAACTGCAGCAAGAAAGCTCTGCACTGAAGAACAAGCTCGAAACTCTATTTAGCTTTGAGCAACAGATCGTGGCTGCTGGCGGGTCAATTCCTACGATGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Hpt

Protein Analysis

153

Amino Acids

17.67

Weight (kDa)

4.96

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hpt PF01627 45 - 129 4.9e-14 Hpt domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 435
AclI AACGTT 1 cut(s) 204
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 2 cut(s) 211, 307
AcuI CTGAAG 2 cut(s) 286, 401
AcyI GRCGYC 1 cut(s) 6
AgsI TTSAA 4 cut(s) 134, 155, 247, 332
AjnI CCWGG 2 cut(s) 185, 260
AluBI AGCT 6 cut(s) 91, 100, 169, 372, 391, 408
AluI AGCT 6 cut(s) 91, 100, 169, 372, 391, 408
Alw26I GTCTC 1 cut(s) 271
AlwNI CAGNNNCTG 2 cut(s) 52, 261
AoxI GGCC 2 cut(s) 183, 318
ApeKI GCWGC 3 cut(s) 100, 361, 428
ApoI RAATTY 2 cut(s) 211, 307
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 319
AsuC2I CCSGG 1 cut(s) 322
AsuII TTCGAA 1 cut(s) 216
BalI TGGCCA 1 cut(s) 185
BanII GRGCYC 1 cut(s) 193
BbvI GCAGC 3 cut(s) 87, 373, 415
BccI CCATC 1 cut(s) 445
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 2 cut(s) 187, 262
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 271
BfaI CTAG 1 cut(s) 194
BfmI CTRYAG 2 cut(s) 101, 359
BisI GCNGC 3 cut(s) 101, 362, 429
BlpI GCTNAGC 1 cut(s) 87
BlsI GCNGC 3 cut(s) 102, 363, 430
Bme1390I CCNGG 3 cut(s) 187, 262, 322
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 1 cut(s) 326
BmrFI CCNGG 3 cut(s) 187, 262, 322
BmsI GCATC 1 cut(s) 303
Bpu1102I GCTNAGC 1 cut(s) 87
Bpu14I TTCGAA 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 322
BsaHI GRCGYC 1 cut(s) 6
BsaJI CCNNGG 1 cut(s) 186
BseBI CCWGG 2 cut(s) 187, 262
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 112
BseMII CTCAG 4 cut(s) 29, 59, 101, 153
BseXI GCAGC 3 cut(s) 87, 373, 415
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 2 cut(s) 185, 320
BsiSI CCGG 1 cut(s) 322
BsmAI GTCTC 1 cut(s) 271
BsnI GGCC 2 cut(s) 185, 320
Bsp119I TTCGAA 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 2 cut(s) 76, 420
Bsp1720I GCTNAGC 1 cut(s) 87
BspACI CCGC 1 cut(s) 435
BspANI GGCC 2 cut(s) 185, 320
BspCNI CTCAG 4 cut(s) 28, 58, 100, 154
BspLI GGNNCC 1 cut(s) 326
BspMAI CTGCAG 2 cut(s) 105, 363
BspT104I TTCGAA 1 cut(s) 216
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 2 cut(s) 76, 420
BssNI GRCGYC 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 187, 262
BstACI GRCGYC 1 cut(s) 6
BstAPI GCANNNNNTGC 1 cut(s) 358
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 2 cut(s) 292, 433
BstDEI CTNAG 4 cut(s) 15, 45, 87, 162
BstF5I GGATG 1 cut(s) 112
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 2 cut(s) 79, 423
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 2 cut(s) 76, 420
BstMWI GCNNNNNNNGC 2 cut(s) 97, 358
BstNI CCWGG 2 cut(s) 187, 262
BstNSI RCATGY 1 cut(s) 294
BstSCI CCNGG 3 cut(s) 185, 260, 320
BstSFI CTRYAG 2 cut(s) 101, 359
BstV1I GCAGC 3 cut(s) 87, 373, 415
BsuRI GGCC 2 cut(s) 185, 320
BtsCI GGATG 1 cut(s) 112
BtsI GCAGTG 2 cut(s) 35, 262
BtsIMutI CAGTG 4 cut(s) 23, 35, 262, 377
Cac8I GCNNGC 2 cut(s) 292, 433
CaiI CAGNNNCTG 2 cut(s) 52, 261
CfoI GCGC 1 cut(s) 57
Cfr13I GGNCC 1 cut(s) 319
CseI GACGC 1 cut(s) 14
CviAII CATG 1 cut(s) 291
DdeI CTNAG 4 cut(s) 15, 45, 87, 162
DpnI GATC 2 cut(s) 78, 422
DpnII GATC 2 cut(s) 76, 420
EaeI YGGCCR 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 193
Eco57I CTGAAG 2 cut(s) 286, 401
EcoRII CCWGG 2 cut(s) 185, 260
EcoT22I ATGCAT 2 cut(s) 292, 296
EcoT38I GRGCYC 1 cut(s) 193
FaeI CATG 1 cut(s) 294
FaiI YATR 3 cut(s) 182, 234, 292
FatI CATG 1 cut(s) 290
FauI CCCGC 1 cut(s) 428
Fnu4HI GCNGC 3 cut(s) 101, 362, 429
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 3 cut(s) 101, 362, 429
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 56
GluI GCNGC 3 cut(s) 101, 362, 429
HaeIII GGCC 2 cut(s) 185, 320
HapII CCGG 1 cut(s) 322
HgaI GACGC 1 cut(s) 14
HhaI GCGC 1 cut(s) 57
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 1 cut(s) 294
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 167
HinfI GANTC 2 cut(s) 13, 66
HpaII CCGG 1 cut(s) 322
Hpy188I TCNGA 1 cut(s) 305
Hpy188III TCNNGA 2 cut(s) 155, 194
HpyAV CCTTC 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 7 cut(s) 103, 272, 290, 294, 352, 361, 377
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 358
HpyF3I CTNAG 4 cut(s) 15, 45, 87, 162
HpySE526I ACGT 1 cut(s) 204
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 1 cut(s) 294
HspAI GCGC 1 cut(s) 55
Kzo9I GATC 2 cut(s) 76, 420
LpnPI CCDG 9 cut(s) 89, 132, 172, 199, 206, 247, 274, 335, 417
Lsp1109I GCAGC 3 cut(s) 87, 373, 415
LweI GCATC 1 cut(s) 303
MaeI CTAG 1 cut(s) 194
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 10
MalI GATC 2 cut(s) 78, 422
MboI GATC 2 cut(s) 76, 420
MboII GAAGA 1 cut(s) 394
MhlI GDGCHC 1 cut(s) 193
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 5 cut(s) 80, 211, 242, 307, 441
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 1 cut(s) 7
MnlI CCTC 2 cut(s) 55, 310
Mox20I TGGCCA 1 cut(s) 185
Mph1103I ATGCAT 2 cut(s) 292, 296
MscI TGGCCA 1 cut(s) 185
MseI TTAA 1 cut(s) 282
Msp20I TGGCCA 1 cut(s) 185
MspI CCGG 1 cut(s) 322
MspR9I CCNGG 3 cut(s) 187, 262, 322
MvaI CCWGG 2 cut(s) 187, 262
MwoI GCNNNNNNNGC 2 cut(s) 97, 358
NciI CCSGG 1 cut(s) 322
NdeII GATC 2 cut(s) 76, 420
NlaIII CATG 1 cut(s) 294
NlaIV GGNNCC 1 cut(s) 326
NmeAIII GCCGAG 1 cut(s) 83
NmuCI GTSAC 1 cut(s) 10
NsiI ATGCAT 2 cut(s) 292, 296
NspI RCATGY 1 cut(s) 294
NspV TTCGAA 1 cut(s) 216
PaeI GCATGC 1 cut(s) 294
PfeI GAWTC 1 cut(s) 66
PkrI GCNGC 3 cut(s) 102, 363, 430
PleI GAGTC 1 cut(s) 7
PpsI GAGTC 1 cut(s) 7
Psp1406I AACGTT 1 cut(s) 204
Psp6I CCWGG 2 cut(s) 185, 260
PspGI CCWGG 2 cut(s) 185, 260
PspN4I GGNNCC 1 cut(s) 326
PspPI GGNCC 1 cut(s) 319
PstI CTGCAG 2 cut(s) 105, 363
PstNI CAGNNNCTG 2 cut(s) 52, 261
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 3 cut(s) 101, 362, 429
Sau3AI GATC 2 cut(s) 76, 420
Sau96I GGNCC 1 cut(s) 319
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 3 cut(s) 187, 262, 322
SduI GDGCHC 1 cut(s) 193
SfaNI GCATC 1 cut(s) 303
SfcI CTRYAG 2 cut(s) 101, 359
SfuI TTCGAA 1 cut(s) 216
SphI GCATGC 1 cut(s) 294
Sse9I AATT 5 cut(s) 80, 211, 242, 307, 441
SsiI CCGC 1 cut(s) 435
SspMI CTAG 1 cut(s) 194
StyD4I CCNGG 3 cut(s) 185, 260, 320
TaiI ACGT 1 cut(s) 207
TaqI TCGA 3 cut(s) 36, 216, 393
TasI AATT 5 cut(s) 80, 211, 242, 307, 441
TfiI GAWTC 1 cut(s) 66
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 4 cut(s) 23, 35, 262, 384
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 3 cut(s) 100, 361, 428
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 123, 227
TspRI CASTG 4 cut(s) 23, 35, 262, 384
XapI RAATTY 2 cut(s) 211, 307
XbaI TCTAGA 1 cut(s) 193
XceI RCATGY 1 cut(s) 294
XspI CTAG 1 cut(s) 194
Zsp2I ATGCAT 2 cut(s) 292, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.