Prupe.8G249400_v2.0.a1

phosphotransfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0007004
Physical Location & Seq
Forward (+)
21517807 .. 21520488
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G249400.1

Sequence Viewer

Length: 459 bp
ATGGATGCTGTGACTCAGTGGCAGAAACAGTGGTTCGACTACACCCAGTCTCTGCGCCGAGAGGGATTCTTGGACGATCAGTTTGCACAGCTAAAGAAGCTCCAGGATGAAAGCAGCCCAGATTTTGTGGTTGAGGTTGTTTCTCTTTTCTTTCAAGATTCTGAGAAGCTTCTCAACAATATGGGCAGGGCTCTAGAACAGAATGTTGTGAATTTCAAACAGGTAGATGCGTACGTCCATCAATTCAAGGGTAGCAGTGCCTGGATAGGTGCATCAAGACTTAAAAATGTATGCATCAACTTCAGAAATTCTTGTGAGGCCCAGAACCTTGAAGGGTGTTTGAGATGTTTGCAACAAGTACAACAAGAAAGCTCTGCATTGAAGAGCAAGCTTGAATACTTATTTATGTTGGAGCAACAGATCGTGGCTGCCGGCGGGTCAATTCCTATTATGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Hpt

Protein Analysis

153

Amino Acids

17.47

Weight (kDa)

5.07

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 435
AcsI RAATTY 2 cut(s) 211, 307
AcuI CTGAAG 1 cut(s) 286
AfaI GTAC 2 cut(s) 233, 360
AgsI TTSAA 6 cut(s) 155, 217, 247, 332, 382, 395
AjnI CCWGG 2 cut(s) 102, 260
AluBI AGCT 5 cut(s) 91, 100, 169, 372, 391
AluI AGCT 5 cut(s) 91, 100, 169, 372, 391
Alw26I GTCTC 1 cut(s) 54
AlwNI CAGNNNCTG 2 cut(s) 52, 261
AoxI GGCC 1 cut(s) 318
ApeKI GCWGC 2 cut(s) 114, 428
ApoI RAATTY 2 cut(s) 211, 307
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 319
BanII GRGCYC 1 cut(s) 193
BbvI GCAGC 2 cut(s) 126, 415
BccI CCATC 1 cut(s) 246
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 2 cut(s) 104, 262
BcoDI GTCTC 1 cut(s) 54
BfaI CTAG 1 cut(s) 194
BisI GCNGC 2 cut(s) 115, 429
BlsI GCNGC 2 cut(s) 116, 430
Bme1390I CCNGG 2 cut(s) 104, 262
BmgT120I GGNCC 1 cut(s) 319
BmrFI CCNGG 2 cut(s) 104, 262
BmrI ACTGGG 1 cut(s) 40
BmsI GCATC 3 cut(s) 217, 281, 303
BmuI ACTGGG 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 86
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 1 cut(s) 46
BseBI CCWGG 2 cut(s) 104, 262
BseGI GGATG 2 cut(s) 10, 112
BseMII CTCAG 2 cut(s) 29, 153
BseNI ACTGG 1 cut(s) 46
BseXI GCAGC 2 cut(s) 126, 415
BshFI GGCC 1 cut(s) 320
BsiSI CCGG 1 cut(s) 432
BsiWI CGTACG 1 cut(s) 231
BsmAI GTCTC 1 cut(s) 54
BsnI GGCC 1 cut(s) 320
Bsp1286I GDGCHC 1 cut(s) 193
Bsp143I GATC 2 cut(s) 76, 420
BspACI CCGC 1 cut(s) 435
BspANI GGCC 1 cut(s) 320
BspCNI CTCAG 2 cut(s) 28, 154
BspQI GCTCTTC 1 cut(s) 377
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 1 cut(s) 46
BssAI RCCGGY 1 cut(s) 431
BssMI GATC 2 cut(s) 76, 420
Bst2UI CCWGG 2 cut(s) 104, 262
Bst4CI ACNGT 1 cut(s) 30
Bst6I CTCTTC 1 cut(s) 377
BstC8I GCNNGC 2 cut(s) 389, 433
BstDEI CTNAG 2 cut(s) 15, 162
BstF5I GGATG 2 cut(s) 10, 112
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 2 cut(s) 79, 423
BstMAI GTCTC 1 cut(s) 54
BstMBI GATC 2 cut(s) 76, 420
BstMWI GCNNNNNNNGC 1 cut(s) 97
BstNI CCWGG 2 cut(s) 104, 262
BstSCI CCNGG 2 cut(s) 102, 260
BstV1I GCAGC 2 cut(s) 126, 415
BsuRI GGCC 1 cut(s) 320
BtsCI GGATG 2 cut(s) 10, 112
BtsI GCAGTG 1 cut(s) 262
BtsIMutI CAGTG 3 cut(s) 23, 35, 262
Cac8I GCNNGC 2 cut(s) 389, 433
CaiI CAGNNNCTG 2 cut(s) 52, 261
CfoI GCGC 1 cut(s) 57
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 319
Csp6I GTAC 2 cut(s) 232, 359
CviJI RGCY 9 cut(s) 91, 100, 117, 169, 191, 320, 372, 391, 428
CviKI_1 RGCY 9 cut(s) 91, 100, 117, 169, 191, 320, 372, 391, 428
CviQI GTAC 2 cut(s) 232, 359
DdeI CTNAG 2 cut(s) 15, 162
DpnI GATC 2 cut(s) 78, 422
DpnII GATC 2 cut(s) 76, 420
Eam1104I CTCTTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 377
Eco24I GRGCYC 1 cut(s) 193
Eco57I CTGAAG 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 102, 260
EcoT22I ATGCAT 1 cut(s) 296
EcoT38I GRGCYC 1 cut(s) 193
FaiI YATR 4 cut(s) 182, 292, 407, 452
FauI CCCGC 1 cut(s) 428
Fnu4HI GCNGC 2 cut(s) 115, 429
FokI GGATG 2 cut(s) 17, 119
FriOI GRGCYC 1 cut(s) 193
Fsp4HI GCNGC 2 cut(s) 115, 429
FspBI CTAG 1 cut(s) 194
GlaI GCGC 1 cut(s) 56
GluI GCNGC 2 cut(s) 115, 429
GsuI CTGGAG 1 cut(s) 86
HaeIII GGCC 1 cut(s) 320
HapII CCGG 1 cut(s) 432
HhaI GCGC 1 cut(s) 57
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HindIII AAGCTT 2 cut(s) 167, 389
HinfI GANTC 3 cut(s) 13, 66, 158
HpaII CCGG 1 cut(s) 432
Hpy188I TCNGA 2 cut(s) 163, 305
Hpy188III TCNNGA 3 cut(s) 155, 194, 276
HpyAV CCTTC 1 cut(s) 326
HpyCH4III ACNGT 1 cut(s) 30
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 5 cut(s) 86, 272, 294, 352, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 97
HpyF3I CTNAG 2 cut(s) 15, 162
HpySE526I ACGT 1 cut(s) 234
HspAI GCGC 1 cut(s) 55
KroI GCCGGC 1 cut(s) 431
KroNI GCCGGC 1 cut(s) 433
Kzo9I GATC 2 cut(s) 76, 420
LguI GCTCTTC 1 cut(s) 377
LmnI GCTCC 2 cut(s) 105, 412
Lsp1109I GCAGC 2 cut(s) 126, 415
LweI GCATC 3 cut(s) 217, 281, 303
MaeI CTAG 1 cut(s) 194
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 10
MalI GATC 2 cut(s) 78, 422
MboI GATC 2 cut(s) 76, 420
MboII GAAGA 1 cut(s) 394
MhlI GDGCHC 1 cut(s) 193
MluCI AATT 4 cut(s) 211, 242, 307, 441
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 3 cut(s) 55, 127, 310
Mph1103I ATGCAT 1 cut(s) 296
MroNI GCCGGC 1 cut(s) 431
MseI TTAA 1 cut(s) 282
MspI CCGG 1 cut(s) 432
MspR9I CCNGG 2 cut(s) 104, 262
MvaI CCWGG 2 cut(s) 104, 262
MwoI GCNNNNNNNGC 1 cut(s) 97
NaeI GCCGGC 1 cut(s) 433
NdeII GATC 2 cut(s) 76, 420
NgoMIV GCCGGC 1 cut(s) 431
NmeAIII GCCGAG 1 cut(s) 83
NmuCI GTSAC 1 cut(s) 10
NsiI ATGCAT 1 cut(s) 296
PciSI GCTCTTC 1 cut(s) 377
PdiI GCCGGC 1 cut(s) 433
PfeI GAWTC 2 cut(s) 66, 158
Pfl23II CGTACG 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 102
PkrI GCNGC 2 cut(s) 116, 430
PleI GAGTC 1 cut(s) 7
PpsI GAGTC 1 cut(s) 7
Psp6I CCWGG 2 cut(s) 102, 260
PspGI CCWGG 2 cut(s) 102, 260
PspLI CGTACG 1 cut(s) 231
PspPI GGNCC 1 cut(s) 319
PstNI CAGNNNCTG 2 cut(s) 52, 261
RsaI GTAC 2 cut(s) 233, 360
RsaNI GTAC 2 cut(s) 232, 359
SapI GCTCTTC 1 cut(s) 377
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 2 cut(s) 115, 429
Sau3AI GATC 2 cut(s) 76, 420
Sau96I GGNCC 1 cut(s) 319
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 104, 262
SduI GDGCHC 1 cut(s) 193
SfaNI GCATC 3 cut(s) 217, 281, 303
Sse9I AATT 4 cut(s) 211, 242, 307, 441
SsiI CCGC 1 cut(s) 435
SspMI CTAG 1 cut(s) 194
StyD4I CCNGG 2 cut(s) 102, 260
TaaI ACNGT 1 cut(s) 30
TaiI ACGT 1 cut(s) 237
TaqI TCGA 1 cut(s) 36
TasI AATT 4 cut(s) 211, 242, 307, 441
TatI WGTACW 1 cut(s) 358
TfiI GAWTC 2 cut(s) 66, 158
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 3 cut(s) 23, 35, 262
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 2 cut(s) 114, 428
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 1 cut(s) 123
TspRI CASTG 3 cut(s) 23, 35, 262
XapI RAATTY 2 cut(s) 211, 307
XbaI TCTAGA 1 cut(s) 193
XspI CTAG 1 cut(s) 194
Zsp2I ATGCAT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.