Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0009030

Physical Location & Seq

Genomic Coordinates Reverse (-)

35659607 .. 35662092

Gene Structure 2486 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1273300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1041 bp

ATGGCTGGTAGTAATGAAGTCAGCGTCAATGAATCCAAGAAAGTTGTTCCGCTCAACACATGGGTACTAATCTCCAATTTCAAGCTAGCTTACAACCTCCTCCGCCGCGCTGACGGAACGTTCAACCGCGAGCTGGCGGAGTTCCTCGAACGGAAAGTCCCCGCCAACGTAACTCCGGTTGACGGGGTTTTCTCGTTTGATCACGTCGACACAGCCACCGGCCTCCTCAACCGTGTTTATAAATTTGCCCCCAAAAACGAGTCGCAATGGGGCATTTCGGATCTTGAGCAGCCCTTGAGCACCACCAAGGTCGTCCCGGTCATAATCTTCTTCCACGGCGGAAGCTTCACACATTCCTCAGCCAACAGCGCCATCTACGACACCTTCTGCCGCCGCCTTGTCAACACTTGCAAGGCTGTTGTAGTCTCTGTAAACTACCGCCGTTCGCCTGAACATCGGTATCCTTGTGCTTATGAAGACGGATGGGCGGCTCTCAAGTGGGTGAAATCGAGAAAGTGGCTGCAGAGCGGGAAGGGTAAGAACTCGAAAGTTCACGTTTACCTAGCTGGAGACAGCTCGGGCGGCAACATTGCTCACCACGTCGCAGTCAAGGCGGCAGAAGCGGAGGTTGAGGTGCTCGGAAACATCCTTCTCCACCCGATGTTTGGGGGGCAGAAGAGAACAGAAACAGAGAAGAGATTGGATGGGAAGTACTTCGTAACAATACAAGACCGTGATTGGTACTGGCGGGCATTTCTGCCAGAAGGCGAAGACAGAGATCATCCGGCGTGTCACGTTTTCGGCCCGAGGGACAAGAGCCTCGAGGGCTTGAAATTTCCCAAGAGTCTTGTTGTTGTGGCCGGTTTCGATCTCATGCAAGATTGGCAATTGGCTTATGTGGAAGGGCTTAAAAACGCAGGGCAGGATGTGAAGCTTCGGTTCTTAAAGCAGGCGACGATCGGGTTCTACTTCTTGCCGAACAACGAGCATTTCTACTGCCTCATGGAGGAGGTGAAGAGCTTCGTGAATCCTGACTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005975 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009056 GO:0009058 GO:0009311 GO:0009312 GO:0009653 GO:0009719 GO:0009725 GO:0009739 GO:0009755 GO:0009791 GO:0009892 GO:0009908 GO:0009937 GO:0009939 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010154 GO:0010325 GO:0010331 GO:0010468 GO:0010476 GO:0010605 GO:0010629 GO:0010646 GO:0010647 GO:0016051 GO:0016787 GO:0019222 GO:0019840 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0031406 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0036094 GO:0042221 GO:0042562 GO:0043167 GO:0043168 GO:0043177 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0043902 GO:0044238 GO:0044424 GO:0044464 GO:0048367 GO:0048437 GO:0048444 GO:0048518 GO:0048519 GO:0048522 GO:0048530 GO:0048583 GO:0048584 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0080154 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:1901576 GO:1901700 GO:1901701 GO:1905392 GO:1905516 GO:2000241 GO:2000243

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Abhydrolase_3

Protein Analysis

347

Amino Acids

39.15

Weight (kDa)

8.59

Isoelectric Point (pI)

37.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BD-FAE	PF20434	94 - 211	8.8e-13	BD-FAE
COesterase	PF00135	99 - 201	2.9e-06	Carboxylesterase family
Abhydrolase_3	PF07859	108 - 323	2.3e-62	alpha/beta hydrolase fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009030)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63010
fragaria_vesca	FvH4_2g24370 FvH4_2g24370 FvH4_3g11720
malus_domestica	MD03G1273300.v1.1 MD11G1296000.v1.1
prunus_persica	Prupe.8G249800_v2.0.a1 Prupe.8G249800_v2.0.a1 Prupe.8G249800_v2.0.a1 Prupe.8G249800_v2.0.a1
pyrus_communis	pycom03g21780 pycom11g26050
rosa_chinensis	RchiOBHm_Chr6g0292241
rosa_laevigata	RLG00000012050
rosa_multiflora	Rmu_sc0002993.1_g000009
rosa_roxburghii	Rroxscaffold_7G00175310
rosa_rugosa	Rorug06G0224800
rosa_samantha	Rh6AG337800 Rh6BG345100 Rh6CG352200 Rh6DG338100
rosa_wichuraiana	Rw6G029420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	240
AbsI	CCTCGAGG	1 cut(s)	821
AccBSI	CCGCTC	2 cut(s)	52, 528
AccI	GTMKAC	1 cut(s)	207
AccII	CGCG	2 cut(s)	108, 129
AclI	AACGTT	1 cut(s)	119
AclWI	GGATC	1 cut(s)	288
AcoI	YGGCCR	1 cut(s)	858
AcsI	RAATTY	2 cut(s)	242, 833
AfaI	GTAC	3 cut(s)	66, 713, 743
AfiI	CCNNNNNNNGG	4 cut(s)	133, 182, 665, 1006
AgsI	TTSAA	3 cut(s)	82, 124, 832
AjiI	CACGTC	2 cut(s)	205, 601
AloI	GAACNNNNNNTCC	2 cut(s)	141, 173
AluBI	AGCT	8 cut(s)	85, 89, 133, 345, 566, 576, 934, 1020
AluI	AGCT	8 cut(s)	85, 89, 133, 345, 566, 576, 934, 1020
Alw21I	GWGCWC	2 cut(s)	302, 639
Alw26I	GTCTC	2 cut(s)	430, 564
AlwI	GGATC	1 cut(s)	288
Ama87I	CYCGRG	3 cut(s)	577, 805, 821
AoxI	GGCC	3 cut(s)	220, 802, 858
ApeKI	GCWGC	2 cut(s)	289, 520
ApoI	RAATTY	2 cut(s)	242, 833
ArsI	GACNNNNNNTTYG	2 cut(s)	141, 173
Asp700I	GAANNNNTTC	2 cut(s)	713, 1019
AspLEI	GCGC	2 cut(s)	110, 371
AspS9I	GGNCC	1 cut(s)	803
AsuC2I	CCSGG	1 cut(s)	317
AsuHPI	GGTGA	3 cut(s)	514, 587, 1024
AsuNHI	GCTAGC	1 cut(s)	85
AvaI	CYCGRG	3 cut(s)	577, 805, 821
BaeI	ACNNNNGTAYC	2 cut(s)	443, 476
BbsI	GAAGAC	2 cut(s)	483, 777
Bbv12I	GWGCWC	2 cut(s)	302, 639
BbvCI	CCTCAGC	1 cut(s)	358
BbvI	GCAGC	2 cut(s)	301, 507
BccI	CCATC	3 cut(s)	380, 477, 698
BceAI	ACGGC	2 cut(s)	352, 426
BciVI	GTATCC	1 cut(s)	471
BclI	TGATCA	1 cut(s)	199
BcnI	CCSGG	1 cut(s)	317
BcoDI	GTCTC	2 cut(s)	430, 564
BfaI	CTAG	2 cut(s)	86, 563
BfmI	CTRYAG	1 cut(s)	521
BfoI	RGCGCY	1 cut(s)	372
BfuI	GTATCC	1 cut(s)	471
BglI	GCCNNNNNGGC	1 cut(s)	825
BisI	GCNGC	8 cut(s)	106, 290, 391, 394, 489, 521, 583, 615
BlsI	GCNGC	8 cut(s)	107, 291, 392, 395, 490, 522, 584, 616
BmcAI	AGTACT	1 cut(s)	713
Bme1390I	CCNGG	1 cut(s)	317
BmeT110I	CYCGRG	3 cut(s)	577, 805, 821
BmgBI	CACGTC	2 cut(s)	205, 601
BmgT120I	GGNCC	1 cut(s)	803
BmrFI	CCNGG	1 cut(s)	317
BmtI	GCTAGC	1 cut(s)	89
BpiI	GAAGAC	2 cut(s)	483, 777
BpmI	CTGGAG	1 cut(s)	588
Bpu10I	CCTNAGC	1 cut(s)	358
BpuEI	CTTGAG	3 cut(s)	305, 316, 479
BpuMI	CCSGG	1 cut(s)	317
BsaJI	CCNNGG	3 cut(s)	306, 334, 806
BsaWI	WCCGGW	1 cut(s)	175
Bsc4I	CCNNNNNNNGG	4 cut(s)	133, 182, 665, 1006
Bse118I	RCCGGY	2 cut(s)	218, 860
Bse1I	ACTGG	1 cut(s)	749
Bse3DI	GCAATG	2 cut(s)	272, 588
BseDI	CCNNGG	3 cut(s)	306, 334, 806
BseGI	GGATG	5 cut(s)	488, 645, 709, 781, 931
BseLI	CCNNNNNNNGG	4 cut(s)	133, 182, 665, 1006
BseMI	GCAATG	2 cut(s)	272, 588
BseMII	CTCAG	1 cut(s)	372
BseNI	ACTGG	1 cut(s)	749
BseRI	GAGGAG	3 cut(s)	89, 215, 1022
BseXI	GCAGC	2 cut(s)	301, 507
Bsh1236I	CGCG	2 cut(s)	108, 129
Bsh1285I	CGRYCG	1 cut(s)	960
BshFI	GGCC	3 cut(s)	222, 804, 860
BsiEI	CGRYCG	1 cut(s)	960
BsiHKAI	GWGCWC	2 cut(s)	302, 639
BsiHKCI	CYCGRG	3 cut(s)	577, 805, 821
BsiSI	CCGG	5 cut(s)	176, 219, 317, 785, 861
BslFI	GGGAC	3 cut(s)	143, 299, 824
BslI	CCNNNNNNNGG	4 cut(s)	133, 182, 665, 1006
BsmAI	GTCTC	2 cut(s)	430, 564
BsmFI	GGGAC	3 cut(s)	143, 299, 824
BsnI	GGCC	3 cut(s)	222, 804, 860
BsoBI	CYCGRG	3 cut(s)	577, 805, 821
Bsp1286I	GDGCHC	2 cut(s)	302, 639
Bsp143I	GATC	5 cut(s)	199, 280, 778, 868, 957
BspANI	GGCC	3 cut(s)	222, 804, 860
BspCNI	CTCAG	1 cut(s)	371
BspFNI	CGCG	2 cut(s)	108, 129
BspMAI	CTGCAG	1 cut(s)	525
BspOI	GCTAGC	1 cut(s)	89
BspPI	GGATC	1 cut(s)	288
BspQI	GCTCTTC	1 cut(s)	1010
BsrBI	CCGCTC	2 cut(s)	52, 528
BsrDI	GCAATG	2 cut(s)	272, 588
BsrFI	RCCGGY	2 cut(s)	218, 860
BsrI	ACTGG	1 cut(s)	749
BssAI	RCCGGY	2 cut(s)	218, 860
BssECI	CCNNGG	3 cut(s)	306, 334, 806
BssMI	GATC	5 cut(s)	199, 280, 778, 868, 957
BssT1I	CCWWGG	1 cut(s)	306
Bst4CI	ACNGT	3 cut(s)	233, 734, 1037
Bst6I	CTCTTC	3 cut(s)	671, 689, 1010
BstC8I	GCNNGC	5 cut(s)	87, 131, 135, 750, 951
BstDEI	CTNAG	1 cut(s)	358
BstDSI	CCRYGG	1 cut(s)	334
BstENI	CCTNNNNNAGG	1 cut(s)	1004
BstF5I	GGATG	5 cut(s)	488, 645, 709, 781, 931
BstFNI	CGCG	2 cut(s)	108, 129
BstH2I	RGCGCY	1 cut(s)	372
BstHHI	GCGC	2 cut(s)	110, 371
BstKTI	GATC	5 cut(s)	202, 283, 781, 871, 960
BstMAI	GTCTC	2 cut(s)	430, 564
BstMBI	GATC	5 cut(s)	199, 280, 778, 868, 957
BstMCI	CGRYCG	1 cut(s)	960
BstMWI	GCNNNNNNNGC	6 cut(s)	368, 582, 611, 620, 825, 883
BstSCI	CCNGG	1 cut(s)	315
BstSFI	CTRYAG	1 cut(s)	521
BstUI	CGCG	2 cut(s)	108, 129
BstV1I	GCAGC	2 cut(s)	301, 507
BstV2I	GAAGAC	2 cut(s)	483, 777
BstX2I	RGATCY	1 cut(s)	280
BstYI	RGATCY	1 cut(s)	280
BsuI	GTATCC	1 cut(s)	471
BsuRI	GGCC	3 cut(s)	222, 804, 860
BtgI	CCRYGG	1 cut(s)	334
BtrI	CACGTC	2 cut(s)	205, 601
BtsCI	GGATG	5 cut(s)	488, 645, 709, 781, 931
Cac8I	GCNNGC	5 cut(s)	87, 131, 135, 750, 951
CfoI	GCGC	2 cut(s)	110, 371
Cfr10I	RCCGGY	2 cut(s)	218, 860
Cfr13I	GGNCC	1 cut(s)	803
CseI	GACGC	1 cut(s)	13
Csp6I	GTAC	3 cut(s)	65, 712, 742
CviAII	CATG	3 cut(s)	60, 874, 1003
CviQI	GTAC	3 cut(s)	65, 712, 742
DdeI	CTNAG	1 cut(s)	358
DpnI	GATC	5 cut(s)	201, 282, 780, 870, 959
DpnII	GATC	5 cut(s)	199, 280, 778, 868, 957
EaeI	YGGCCR	1 cut(s)	858
Eam1104I	CTCTTC	3 cut(s)	671, 689, 1010
EarI	CTCTTC	3 cut(s)	671, 689, 1010
EciI	GGCGGA	3 cut(s)	92, 152, 354
Eco130I	CCWWGG	1 cut(s)	306
Eco88I	CYCGRG	3 cut(s)	577, 805, 821
EcoNI	CCTNNNNNAGG	1 cut(s)	1004
EcoT14I	CCWWGG	1 cut(s)	306
ErhI	CCWWGG	1 cut(s)	306
FaeI	CATG	3 cut(s)	63, 877, 1006
FaiI	YATR	7 cut(s)	61, 240, 323, 474, 875, 897, 1004
FaqI	GGGAC	3 cut(s)	143, 299, 824
FatI	CATG	3 cut(s)	59, 873, 1002
FauI	CCCGC	3 cut(s)	169, 521, 741
FbaI	TGATCA	1 cut(s)	199
FblI	GTMKAC	1 cut(s)	207
Fnu4HI	GCNGC	8 cut(s)	106, 290, 391, 394, 489, 521, 583, 615
FokI	GGATG	5 cut(s)	495, 632, 716, 768, 938
Fsp4HI	GCNGC	8 cut(s)	106, 290, 391, 394, 489, 521, 583, 615
FspBI	CTAG	2 cut(s)	86, 563
GlaI	GCGC	2 cut(s)	109, 370
GluI	GCNGC	8 cut(s)	106, 290, 391, 394, 489, 521, 583, 615
GsuI	CTGGAG	1 cut(s)	588
HaeII	RGCGCY	1 cut(s)	372
HaeIII	GGCC	3 cut(s)	222, 804, 860
HapII	CCGG	5 cut(s)	176, 219, 317, 785, 861
HgaI	GACGC	1 cut(s)	13
HhaI	GCGC	2 cut(s)	110, 371
Hin1II	CATG	3 cut(s)	63, 877, 1006
Hin6I	GCGC	2 cut(s)	108, 369
HinP1I	GCGC	2 cut(s)	108, 369
HincII	GTYRAC	3 cut(s)	181, 208, 403
HindII	GTYRAC	3 cut(s)	181, 208, 403
HindIII	AAGCTT	2 cut(s)	343, 932
HinfI	GANTC	4 cut(s)	32, 260, 844, 1027
HpaII	CCGG	5 cut(s)	176, 219, 317, 785, 861
HphI	GGTGA	3 cut(s)	514, 587, 1024
Hpy166II	GTNNAC	6 cut(s)	181, 208, 403, 433, 553, 559
Hpy188I	TCNGA	2 cut(s)	280, 641
Hpy188III	TCNNGA	4 cut(s)	284, 510, 1024, 1031
Hpy8I	GTNNAC	6 cut(s)	181, 208, 403, 433, 553, 559
Hpy99I	CGWCG	3 cut(s)	209, 605, 958
HpyAV	CCTTC	5 cut(s)	394, 526, 659, 758, 896
HpyCH4III	ACNGT	3 cut(s)	233, 734, 1037
HpyCH4IV	ACGT	6 cut(s)	119, 168, 204, 555, 600, 795
HpyCH4V	TGCA	3 cut(s)	411, 523, 877
HpyF10VI	GCNNNNNNNGC	6 cut(s)	368, 582, 611, 620, 825, 883
HpyF3I	CTNAG	1 cut(s)	358
HpySE526I	ACGT	6 cut(s)	119, 168, 204, 555, 600, 795
Hsp92II	CATG	3 cut(s)	63, 877, 1006
HspAI	GCGC	2 cut(s)	108, 369
Ksp22I	TGATCA	1 cut(s)	199
Kzo9I	GATC	5 cut(s)	199, 280, 778, 868, 957
LguI	GCTCTTC	1 cut(s)	1010
Lsp1109I	GCAGC	2 cut(s)	301, 507
MaeI	CTAG	2 cut(s)	86, 563
MaeII	ACGT	6 cut(s)	119, 168, 204, 555, 600, 795
MaeIII	GTNAC	3 cut(s)	169, 718, 791
MalI	GATC	5 cut(s)	201, 282, 780, 870, 959
MbiI	CCGCTC	2 cut(s)	52, 528
MboI	GATC	5 cut(s)	199, 280, 778, 868, 957
MboII	GAAGA	7 cut(s)	319, 322, 488, 688, 706, 782, 1027
MfeI	CAATTG	1 cut(s)	887
MflI	RGATCY	1 cut(s)	280
MhlI	GDGCHC	2 cut(s)	302, 639
MluCI	AATT	4 cut(s)	76, 242, 833, 887
MlyI	GAGTC	2 cut(s)	269, 853
MroXI	GAANNNNTTC	2 cut(s)	713, 1019
MseI	TTAA	3 cut(s)	909, 944, 1039
MspI	CCGG	5 cut(s)	176, 219, 317, 785, 861
MspR9I	CCNGG	1 cut(s)	317
MunI	CAATTG	1 cut(s)	887
MvnI	CGCG	2 cut(s)	108, 129
MwoI	GCNNNNNNNGC	6 cut(s)	368, 582, 611, 620, 825, 883
NciI	CCSGG	1 cut(s)	317
NdeII	GATC	5 cut(s)	199, 280, 778, 868, 957
NheI	GCTAGC	1 cut(s)	85
NlaIII	CATG	3 cut(s)	63, 877, 1006
NmuCI	GTSAC	1 cut(s)	791
PaeR7I	CTCGAG	1 cut(s)	821
PciSI	GCTCTTC	1 cut(s)	1010
PdmI	GAANNNNTTC	2 cut(s)	713, 1019
PfeI	GAWTC	2 cut(s)	32, 1027
PkrI	GCNGC	8 cut(s)	107, 291, 392, 395, 490, 522, 584, 616
Ple19I	CGATCG	1 cut(s)	960
PleI	GAGTC	2 cut(s)	268, 852
PpsI	GAGTC	2 cut(s)	268, 852
PsiI	TTATAA	1 cut(s)	240
Psp1406I	AACGTT	1 cut(s)	119
PspPI	GGNCC	1 cut(s)	803
PspXI	VCTCGAGB	1 cut(s)	821
PstI	CTGCAG	1 cut(s)	525
PsuI	RGATCY	1 cut(s)	280
PvuI	CGATCG	1 cut(s)	960
RsaI	GTAC	3 cut(s)	66, 713, 743
RsaNI	GTAC	3 cut(s)	65, 712, 742
SalI	GTCGAC	1 cut(s)	206
SapI	GCTCTTC	1 cut(s)	1010
SaqAI	TTAA	3 cut(s)	909, 944, 1039
SatI	GCNGC	8 cut(s)	106, 290, 391, 394, 489, 521, 583, 615
Sau3AI	GATC	5 cut(s)	199, 280, 778, 868, 957
Sau96I	GGNCC	1 cut(s)	803
ScaI	AGTACT	1 cut(s)	713
SchI	GAGTC	2 cut(s)	269, 853
ScrFI	CCNGG	1 cut(s)	317
SduI	GDGCHC	2 cut(s)	302, 639
SfcI	CTRYAG	1 cut(s)	521
Sfr274I	CTCGAG	1 cut(s)	821
SlaI	CTCGAG	1 cut(s)	821
SmlI	CTYRAG	4 cut(s)	284, 295, 494, 821
SmoI	CTYRAG	4 cut(s)	284, 295, 494, 821
Sse9I	AATT	4 cut(s)	76, 242, 833, 887
SspMI	CTAG	2 cut(s)	86, 563
StyD4I	CCNGG	1 cut(s)	315
StyI	CCWWGG	1 cut(s)	306
TaaI	ACNGT	3 cut(s)	233, 734, 1037
TaiI	ACGT	6 cut(s)	122, 171, 207, 558, 603, 798
TaqI	TCGA	6 cut(s)	147, 207, 509, 545, 822, 867
TasI	AATT	4 cut(s)	76, 242, 833, 887
TatI	WGTACW	1 cut(s)	711
TauI	GCSGC	6 cut(s)	108, 393, 396, 491, 585, 617
TfiI	GAWTC	2 cut(s)	32, 1027
Tru1I	TTAA	3 cut(s)	909, 944, 1039
Tru9I	TTAA	3 cut(s)	909, 944, 1039
TseFI	GTSAC	1 cut(s)	791
TseI	GCWGC	2 cut(s)	289, 520
Tsp45I	GTSAC	1 cut(s)	791
TspDTI	ATGAA	3 cut(s)	30, 45, 489
TspGWI	ACGGA	3 cut(s)	129, 166, 495
XagI	CCTNNNNNAGG	1 cut(s)	1004
XapI	RAATTY	2 cut(s)	242, 833
XcmI	CCANNNNNNNNNTGG	1 cut(s)	662
XhoI	CTCGAG	1 cut(s)	821
XmiI	GTMKAC	1 cut(s)	207
XmnI	GAANNNNTTC	2 cut(s)	713, 1019
XspI	CTAG	2 cut(s)	86, 563
ZrmI	AGTACT	1 cut(s)	713

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.