MD03G1277800.v1.1

May serve as docking site to facilitate the association of other proteins to the plasma membrane

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013616
Physical Location & Seq
Reverse (-)
35989308 .. 35992347
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1277800.v1.1.491

Sequence Viewer

Length: 363 bp
ATGGGGCAAAGAGGGGAGCATATAGCACTCAAGTTCCGGATTTACGACGGGACAGATATAGGACACAATAGTTATGCACCATCTATGACGGTATCATCTCTTAAGAAAAGGCTACTTGATGAGTGGCCTCAAGCAAAAACAGTGACGCCGAAGTCAGTAGACGAAGTGAAAATCATACATGGGGGCAGAGTTTTAGAGAATAGCAAGACACTTGCTGATTCCAGAATAACCGCCGGTAATCAGACCGGTGAGGCTGTGATCATGCATGCCGTAGTACAGCCTCTTGTACCCAAAAACAAAAAGACAGGTAAAAACCAGAAAGATATGCAAAAGATGAACTCATGCTCTTGCACCCTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rad60-SLD_2

Protein Analysis

121

Amino Acids

13.22

Weight (kDa)

9.61

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD_2 PF13881 6 - 118 3.6e-34 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 151
AccI GTMKAC 1 cut(s) 159
AccIII TCCGGA 1 cut(s) 36
AciI CCGC 1 cut(s) 231
AcyI GRCGYC 1 cut(s) 146
AfaI GTAC 2 cut(s) 276, 288
AflII CTTAAG 1 cut(s) 101
AgeI ACCGGT 1 cut(s) 245
Aor13HI TCCGGA 1 cut(s) 36
AoxI GGCC 1 cut(s) 125
AsiGI ACCGGT 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 260
BccI CCATC 1 cut(s) 88
BceAI ACGGC 1 cut(s) 254
BclI TGATCA 1 cut(s) 258
BfrI CTTAAG 1 cut(s) 101
BpuEI CTTGAG 2 cut(s) 14, 114
BsaHI GRCGYC 1 cut(s) 146
BsaWI WCCGGW 2 cut(s) 36, 245
Bse118I RCCGGY 2 cut(s) 233, 245
BseAI TCCGGA 1 cut(s) 36
BshFI GGCC 1 cut(s) 127
BshTI ACCGGT 1 cut(s) 245
BsiSI CCGG 3 cut(s) 37, 234, 246
BslFI GGGAC 1 cut(s) 64
BsmFI GGGAC 1 cut(s) 64
BsnI GGCC 1 cut(s) 127
Bsp13I TCCGGA 1 cut(s) 36
Bsp143I GATC 1 cut(s) 258
BspACI CCGC 1 cut(s) 231
BspANI GGCC 1 cut(s) 127
BspEI TCCGGA 1 cut(s) 36
BspTI CTTAAG 1 cut(s) 101
BsrFI RCCGGY 2 cut(s) 233, 245
BssAI RCCGGY 2 cut(s) 233, 245
BssMI GATC 1 cut(s) 258
BssNI GRCGYC 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 91, 142
BstACI GRCGYC 1 cut(s) 146
BstAFI CTTAAG 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 267
BstKTI GATC 1 cut(s) 261
BstMBI GATC 1 cut(s) 258
BstNSI RCATGY 1 cut(s) 269
BsuRI GGCC 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 267
Cfr10I RCCGGY 2 cut(s) 233, 245
CseI GACGC 1 cut(s) 154
Csp6I GTAC 2 cut(s) 275, 287
CspAI ACCGGT 1 cut(s) 245
CviAII CATG 4 cut(s) 179, 262, 266, 342
CviJI RGCY 4 cut(s) 112, 127, 254, 280
CviKI_1 RGCY 4 cut(s) 112, 127, 254, 280
CviQI GTAC 2 cut(s) 275, 287
DpnI GATC 1 cut(s) 260
DpnII GATC 1 cut(s) 258
DrdI GACNNNNNNGTC 1 cut(s) 151
DseDI GACNNNNNNGTC 1 cut(s) 151
EcoT22I ATGCAT 1 cut(s) 267
FaeI CATG 4 cut(s) 182, 265, 269, 345
FaqI GGGAC 1 cut(s) 64
FatI CATG 4 cut(s) 178, 261, 265, 341
FbaI TGATCA 1 cut(s) 258
FblI GTMKAC 1 cut(s) 159
HaeIII GGCC 1 cut(s) 127
HapII CCGG 3 cut(s) 37, 234, 246
HgaI GACGC 1 cut(s) 154
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 4 cut(s) 182, 265, 269, 345
HinfI GANTC 1 cut(s) 218
HpaII CCGG 3 cut(s) 37, 234, 246
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 2 cut(s) 37, 222
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 50
HpyCH4III ACNGT 2 cut(s) 91, 142
HpyCH4V TGCA 4 cut(s) 77, 265, 328, 351
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 4 cut(s) 182, 265, 269, 345
Kpn2I TCCGGA 1 cut(s) 36
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 1 cut(s) 258
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 6 cut(s) 50, 235, 247, 259, 291, 329
MaeIII GTNAC 1 cut(s) 142
MalI GATC 1 cut(s) 260
MboI GATC 1 cut(s) 258
MnlI CCTC 4 cut(s) 5, 138, 244, 291
Mph1103I ATGCAT 1 cut(s) 267
MroI TCCGGA 1 cut(s) 36
MseI TTAA 2 cut(s) 102, 361
MspCI CTTAAG 1 cut(s) 101
MspI CCGG 3 cut(s) 37, 234, 246
NdeII GATC 1 cut(s) 258
NlaIII CATG 4 cut(s) 182, 265, 269, 345
NmuCI GTSAC 1 cut(s) 142
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 1 cut(s) 269
PaeI GCATGC 1 cut(s) 269
PfeI GAWTC 1 cut(s) 218
PinAI ACCGGT 1 cut(s) 245
RsaI GTAC 2 cut(s) 276, 288
RsaNI GTAC 2 cut(s) 275, 287
SaqAI TTAA 2 cut(s) 102, 361
Sau3AI GATC 1 cut(s) 258
SetI ASST 1 cut(s) 310
SmlI CTYRAG 3 cut(s) 29, 101, 129
SmoI CTYRAG 3 cut(s) 29, 101, 129
SphI GCATGC 1 cut(s) 269
SsiI CCGC 1 cut(s) 231
TaaI ACNGT 2 cut(s) 91, 142
TatI WGTACW 1 cut(s) 274
TfiI GAWTC 1 cut(s) 218
Tru1I TTAA 2 cut(s) 102, 361
Tru9I TTAA 2 cut(s) 102, 361
TscAI CASTG 1 cut(s) 147
TseFI GTSAC 1 cut(s) 142
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 1 cut(s) 350
TspRI CASTG 1 cut(s) 147
Vha464I CTTAAG 1 cut(s) 101
XceI RCATGY 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 159
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.