MD03G1280600.v1.1

Spermatogenesis-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012290
Physical Location & Seq
Forward (+)
36170721 .. 36176163
5443 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1280600.v1.1.491

Sequence Viewer

Length: 2514 bp
ATGGCACCAACTTCTGCACTCTTCTCTTCTTCTCTCTCTCACTCCTCTCTGCTTCTCCACAATTCTATGCTCAAACGCCTCTCTCTCCGCCGCTTCCTCTCTCATCCAACCAAACAGCTCTCCTCTCCTCCATCACCACCGCCCCAATCTCCCTCCACTCACTTTCCCGCCCTTTCCTCACTGATTCCCCGCCGACCCTTCTCCGCCTCCAAGCTCCTCGCCATGGCCGAGCAAACTTCGAGCAACCCGGCTGCCCCGTCTCACAAGCACTCCAATCGCCTCGCCGCCGAGCACAGCCCCTACCTTCTGCAACACGCCCACAACCCGGTGGATTGGTATCCGTGGGGAGAAGAAGCTTTCGCCGAAGCTCGAAAGAGGGACGTTCCCATCTTCTTATCAATCGGATACAGCACGTGTCATTGGTGTCATGTTATGGAGGTCGAGTCTTTCGAGGATGAAGGCGTTGCGAAATTGCTGAACGATTGGTTTGTTAGTATCAAGGTGGACCGGGAGGAAAGACCAGATGTTGATAAGGTATACATGACCTATGTACAGGCTCTGTATGGTGGTGGAGGTTGGCCGCTGAGTGTCTTCCTTTCACCTGATTTGAAACCTTTGATGGGTGGAACTTACTTTCCCCCAGACGATAAGTTTGGAAGACCAGGATTTAAGACTATACTTAGAAAGGTGAAAGATGCTTGGGATAGTAAGAGGGATGTGCTTGTTAAAAGTGGAGCATTTGCGATTGAACAGCTGTCAGAAGCATTGTCGTCAAGTGCTAGTTCTAATAAATTGCCAGATGGGCTTCCACAGATTGCATTGCGTCTATGTGCTGAGCAACTTTCCGGAAGCTATGATGAAAAGTATGGTGGGTTCGGATCCGCCCCAAAGTTTCCTAGACCAGTTGAGGTTCAGCTCATGCTTTATTACTCAAAGAAGTTGGAGGAAACTGGGAAGTTGGGTGAGGCAAATAAAAATCTGAAAATGGTTTTCTTCACCTTGCAATGCATGGCAAAAGGGGGTGTCCACGATCATATTGGAGGTGGCTTCCACAGATATAGCGTGGATGAGTGCTGGCATGTCCCGCACTTTGAGAAGATGTTATATGATCAAGGGCAGATTGCCAATGTCTATTTAGAAGCTTTTTCTATTACCAAAGATGTCTTCTATTCATGTGTTGCACGGGATGTTCTTGATTATCTGAGGAGAGAAATGATTGGACCGGAAGGCGAAATCTATTCAGCGGAAGATGCTGACAGTGCTGAATCTGAAGGTGCTACAAGAAAAAAGGAAGGAGCCTTCTATGTTTGGACTAAAAAAGAGGTTGAAGACATACTTGGAGAGGATGCTACTCTTTTTAAGAACCATTATTATATAAAGCCTTCGGGAAACTGTGACCTTTCTAGAATGAGTGATCCTCACAATGAATTCAAGGGAACGAATGTGCTGATTGAGAGAAAAAGCCCGTCTGAATTGGCACCAAAATTTGCCATGCCTGTTGAGAAATACCTTGATATTCTGGGCCAGAGCAGGAAAAAGCTTTTTGAAGTACGATCTACTCGACCAAGACCACATTTAGATGATAAGGTAATTGTGTCATGGAATGGACTGGCTATCTCAGCTTTTGCAAGGGCTTCTAAAATTCTCAAGAATGAACCTGATGGGATCAAATTTAACTTTCCCGTGGTCGGCTCTGATCCAAAGGAGTACATGCAGGTTGCAGAGAGGGCTGCAACTTTTATCAGGAGAAAGCTTTACGACGAGCAAACTCATCGACTACAGCACAGCTTTAGGAATGGTCCATCTAAAGCACCTGGATTTTTAGATGATTATGCTTTTCTCATATCGGGGTTGTTGGATCTTTACGAATTTGGTGGTCAAACTACCTGGCTAATTTGGGCAACTGAACTGCAAGACACCCAGGATGAATTATTTCTTGATAGAGAGGGAGGCGGCTATTTTAATACACCAGGGGAAGACCCCAATATTCTACTTCGTGTGAAGGAAGATCACGATGGGGCTGAACCCTCTGGAAACTCGGTTTCTGTGATCAATCTTGTAAGATTGGCATCCATGATAGCTGGAAACAAGTCTGATCATTACAGGAGGGATGCCGATCATGTTCTTGCTGTTTTTGAGACGAGATTAAAAGACATGGCGATGGCAGTACCTTTGATGTGTTGTGCAGCAGACATGCTCGCTGTTCCTTCCCGAAAGCAAGTTGTCTTGGTTGGCCGGAAGCATTCTGCAGAGTTCAAAAACATGCTTGCCGCCACACATGCATCATACGACACAAACAAAACAGTTATTCACATCGATCCAGCCAATTCAGAAGAAATGCAATTTTGGGAAAGCAACAATAGCAACATTGCTCTGATGGCAAAGAATCATTTTGCTGCGGACAAAGTGGTCGCTCTTGTCTGCCAAAACTTCACTTGCAGTGCTCCTGCGACGGACCCCGGATCCCTCAAGGCGTTGCTCTCCGGAAAATCATCCCCCTCCCCTTCTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
Thioredox_DsbH

Protein Analysis

838

Amino Acids

93.4

Weight (kDa)

6.42

Isoelectric Point (pI)

51.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredox_DsbH PF03190 92 - 251 3.8e-75 Protein of unknown function, DUF255
Thioredoxin_7 PF13899 111 - 176 6.7e-09 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012290)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03200 AT4G03200
fragaria_vesca FvH4_2g25030
malus_domestica MD03G1280600.v1.1
prunus_persica Prupe.8G255100_v2.0.a1
pyrus_communis pycom03g22230
rosa_chinensis RchiOBHm_Chr6g0293001
rosa_laevigata RLG00000011975
rosa_multiflora Rmu_sc0001788.1_g000005 Rmu_sc0003615.1_g000002 Rmu_sc0028741.1_g000001
rosa_roxburghii Rroxscaffold_7G00174480
rosa_rugosa Rorug06G0231900 Rorug06G0232000
rosa_samantha Rh6AG344700 Rh6BG352100 Rh6CG359300 Rh6DG344800
rosa_wichuraiana Rw6G030080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2408
Acc36I ACCTGC 1 cut(s) 1705
AccB1I GGYRCC 2 cut(s) 4, 1477
AccI GTMKAC 1 cut(s) 537
AccIII TCCGGA 2 cut(s) 845, 2483
AclWI GGATC 9 cut(s) 873, 886, 1409, 1673, 1691, 1866, 2312, 2457, 2470
AcoI YGGCCR 3 cut(s) 225, 578, 2233
AcsI RAATTY 5 cut(s) 1427, 1484, 1641, 1670, 1868
AcuI CTGAAG 1 cut(s) 1290
AcvI CACGTG 1 cut(s) 414
AfaI GTAC 4 cut(s) 552, 1551, 1709, 2169
AfiI CCNNNNNNNGG 4 cut(s) 223, 325, 620, 1688
AflIII ACRYGT 1 cut(s) 413
AgsI TTSAA 6 cut(s) 610, 749, 1328, 1432, 1547, 2257
AjnI CCWGG 5 cut(s) 661, 1813, 1886, 1920, 1969
AjuI GAANNNNNNNTTGG 4 cut(s) 1148, 1180, 1320, 1352
AloI GAACNNNNNNTCC 2 cut(s) 619, 651
Alw21I GWGCWC 2 cut(s) 294, 2446
Alw26I GTCTC 2 cut(s) 264, 2132
AlwI GGATC 9 cut(s) 873, 886, 1409, 1673, 1691, 1866, 2312, 2457, 2470
AlwNI CAGNNNCTG 1 cut(s) 559
Aor13HI TCCGGA 2 cut(s) 845, 2483
AoxI GGCC 4 cut(s) 225, 578, 1522, 2233
ApeKI GCWGC 4 cut(s) 251, 1730, 2186, 2396
ApoI RAATTY 5 cut(s) 1427, 1484, 1641, 1670, 1868
ArsI GACNNNNNNTTYG 2 cut(s) 635, 667
Asp700I GAANNNNTTC 1 cut(s) 1932
AspS9I GGNCC 5 cut(s) 505, 1220, 1522, 1799, 2455
AsuC2I CCSGG 4 cut(s) 248, 326, 509, 2460
AsuHPI GGTGA 5 cut(s) 126, 591, 700, 974, 988
AvaII GGWCC 4 cut(s) 505, 1220, 1799, 2455
BamHI GGATCC 2 cut(s) 878, 2462
BanI GGYRCC 2 cut(s) 4, 1477
BbrPI CACGTG 1 cut(s) 414
BbsI GAAGAC 5 cut(s) 583, 664, 1156, 1335, 1983
Bbv12I GWGCWC 2 cut(s) 294, 2446
BbvI GCAGC 4 cut(s) 238, 1717, 2198, 2383
BccI CCATC 9 cut(s) 139, 395, 613, 794, 1655, 1810, 2009, 2155, 2371
BcgI CGANNNNNNTGC 4 cut(s) 257, 291, 1754, 1788
BciT130I CCWGG 5 cut(s) 663, 1815, 1888, 1922, 1971
BciVI GTATCC 2 cut(s) 348, 398
BclI TGATCA 3 cut(s) 1108, 2049, 2095
BcnI CCSGG 4 cut(s) 248, 326, 509, 2460
BcoDI GTCTC 2 cut(s) 264, 2132
BfaI CTAG 3 cut(s) 780, 897, 1404
BfmI CTRYAG 2 cut(s) 1778, 2247
BfuAI ACCTGC 1 cut(s) 1705
BfuI GTATCC 2 cut(s) 348, 398
BglI GCCNNNNNGGC 1 cut(s) 802
BisI GCNGC 9 cut(s) 91, 252, 285, 581, 1731, 1954, 2187, 2271, 2397
BlpI GCTNAGC 1 cut(s) 834
BlsI GCNGC 9 cut(s) 92, 253, 286, 582, 1732, 1955, 2188, 2272, 2398
Bme1390I CCNGG 9 cut(s) 248, 326, 509, 663, 1815, 1888, 1922, 1971, 2460
Bme18I GGWCC 4 cut(s) 505, 1220, 1799, 2455
BmgT120I GGNCC 5 cut(s) 505, 1220, 1522, 1799, 2455
BmiI GGNNCC 6 cut(s) 6, 880, 1297, 1479, 2457, 2464
BmrFI CCNGG 9 cut(s) 248, 326, 509, 663, 1815, 1888, 1922, 1971, 2460
BmrI ACTGGG 1 cut(s) 960
BmsI GCATC 6 cut(s) 685, 1240, 1336, 2078, 2101, 2291
BmuI ACTGGG 1 cut(s) 960
BpiI GAAGAC 5 cut(s) 583, 664, 1156, 1335, 1983
BplI GAGNNNNNCTC 2 cut(s) 1402, 1434
Bpu1102I GCTNAGC 1 cut(s) 834
BpuEI CTTGAG 2 cut(s) 1631, 2453
BpuMI CCSGG 4 cut(s) 248, 326, 509, 2460
Bsa29I ATCGAT 1 cut(s) 2316
BsaAI YACGTR 1 cut(s) 414
BsaBI GATNNNNATC 3 cut(s) 336, 2068, 2115
BsaJI CCNNGG 6 cut(s) 222, 341, 1683, 1920, 1970, 2458
BsaWI WCCGGW 3 cut(s) 845, 1222, 2483
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 428, 458
Bsc4I CCNNNNNNNGG 4 cut(s) 223, 325, 620, 1688
Bse1I ACTGG 3 cut(s) 902, 955, 1614
Bse3DI GCAATG 3 cut(s) 818, 1010, 2367
Bse8I GATNNNNATC 3 cut(s) 336, 2068, 2115
BseAI TCCGGA 2 cut(s) 845, 2483
BseBI CCWGG 5 cut(s) 663, 1815, 1888, 1922, 1971
BseCI ATCGAT 1 cut(s) 2316
BseDI CCNNGG 6 cut(s) 222, 341, 1683, 1920, 1970, 2458
BseJI GATNNNNATC 3 cut(s) 336, 2068, 2115
BseLI CCNNNNNNNGG 4 cut(s) 223, 325, 620, 1688
BseMI GCAATG 3 cut(s) 818, 1010, 2367
BseMII CTCAG 4 cut(s) 575, 825, 1193, 1632
BseNI ACTGG 3 cut(s) 902, 955, 1614
BseRI GAGGAG 5 cut(s) 34, 112, 117, 206, 1219
BseXI GCAGC 4 cut(s) 238, 1717, 2198, 2383
BsgI GTGCAG 1 cut(s) 2205
BshFI GGCC 4 cut(s) 227, 580, 1524, 2235
BshNI GGYRCC 2 cut(s) 4, 1477
BshVI ATCGAT 1 cut(s) 2316
BsiHKAI GWGCWC 2 cut(s) 294, 2446
BsiSI CCGG 8 cut(s) 248, 326, 508, 846, 1223, 2236, 2460, 2484
BslFI GGGAC 2 cut(s) 392, 1067
BslI CCNNNNNNNGG 4 cut(s) 223, 325, 620, 1688
BsmAI GTCTC 2 cut(s) 264, 2132
BsmBI CGTCTC 2 cut(s) 264, 2132
BsmFI GGGAC 2 cut(s) 392, 1067
BsmI GAATGC 1 cut(s) 2242
BsnI GGCC 4 cut(s) 227, 580, 1524, 2235
Bsp1286I GDGCHC 2 cut(s) 294, 2446
Bsp13I TCCGGA 2 cut(s) 845, 2483
Bsp1407I TGTACA 1 cut(s) 550
Bsp1720I GCTNAGC 1 cut(s) 834
Bsp19I CCATGG 1 cut(s) 222
BspANI GGCC 4 cut(s) 227, 580, 1524, 2235
BspCNI CTCAG 4 cut(s) 576, 826, 1194, 1631
BspDI ATCGAT 1 cut(s) 2316
BspEI TCCGGA 2 cut(s) 845, 2483
BspLI GGNNCC 6 cut(s) 6, 880, 1297, 1479, 2457, 2464
BspMAI CTGCAG 1 cut(s) 2251
BspMI ACCTGC 1 cut(s) 1705
BspPI GGATC 9 cut(s) 873, 886, 1409, 1673, 1691, 1866, 2312, 2457, 2470
BspT107I GGYRCC 2 cut(s) 4, 1477
BsrDI GCAATG 3 cut(s) 818, 1010, 2367
BsrGI TGTACA 1 cut(s) 550
BsrI ACTGG 3 cut(s) 902, 955, 1614
BssECI CCNNGG 6 cut(s) 222, 341, 1683, 1920, 1970, 2458
BssNAI GTATAC 1 cut(s) 538
BssT1I CCWWGG 1 cut(s) 222
Bst1107I GTATAC 1 cut(s) 538
Bst2UI CCWGG 5 cut(s) 663, 1815, 1888, 1922, 1971
Bst4CI ACNGT 3 cut(s) 1259, 1394, 2305
Bst6I CTCTTC 2 cut(s) 26, 31
BstAUI TGTACA 1 cut(s) 550
BstBAI YACGTR 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 1076, 2199, 2268
BstDEI CTNAG 5 cut(s) 584, 680, 834, 1202, 1618
BstDSI CCRYGG 3 cut(s) 222, 341, 1683
BstMAI GTCTC 2 cut(s) 264, 2132
BstMWI GCNNNNNNNGC 9 cut(s) 802, 1084, 1250, 1259, 1619, 1727, 2279, 2361, 2378
BstNI CCWGG 5 cut(s) 663, 1815, 1888, 1922, 1971
BstNSI RCATGY 5 cut(s) 1082, 1714, 2197, 2266, 2282
BstSCI CCNGG 9 cut(s) 246, 324, 507, 661, 1813, 1886, 1920, 1969, 2458
BstSFI CTRYAG 2 cut(s) 1778, 2247
BstV1I GCAGC 4 cut(s) 238, 1717, 2198, 2383
BstV2I GAAGAC 5 cut(s) 583, 664, 1156, 1335, 1983
BstX2I RGATCY 3 cut(s) 878, 1858, 2462
BstYI RGATCY 3 cut(s) 878, 1858, 2462
BstZ17I GTATAC 1 cut(s) 538
Bsu15I ATCGAT 1 cut(s) 2316
BsuI GTATCC 2 cut(s) 348, 398
BsuRI GGCC 4 cut(s) 227, 580, 1524, 2235
BsuTUI ATCGAT 1 cut(s) 2316
BtgI CCRYGG 3 cut(s) 222, 341, 1683
BtgZI GCGATG 1 cut(s) 2174
BtsI GCAGTG 1 cut(s) 2446
BtsIMutI CAGTG 3 cut(s) 179, 1264, 2446
BveI ACCTGC 1 cut(s) 1705
Cac8I GCNNGC 3 cut(s) 1076, 2199, 2268
CaiI CAGNNNCTG 1 cut(s) 559
Cfr13I GGNCC 5 cut(s) 505, 1220, 1522, 1799, 2455
ClaI ATCGAT 1 cut(s) 2316
CseI GACGC 1 cut(s) 812
Csp6I GTAC 4 cut(s) 551, 1550, 1708, 2168
CviQI GTAC 4 cut(s) 551, 1550, 1708, 2168
DdeI CTNAG 5 cut(s) 584, 680, 834, 1202, 1618
DrdI GACNNNNNNGTC 1 cut(s) 2408
DseDI GACNNNNNNGTC 1 cut(s) 2408
EaeI YGGCCR 3 cut(s) 225, 578, 2233
Eam1104I CTCTTC 2 cut(s) 26, 31
EarI CTCTTC 2 cut(s) 26, 31
EciI GGCGGA 3 cut(s) 77, 193, 871
Eco130I CCWWGG 1 cut(s) 222
Eco47I GGWCC 4 cut(s) 505, 1220, 1799, 2455
Eco57I CTGAAG 1 cut(s) 1290
Eco72I CACGTG 1 cut(s) 414
EcoRI GAATTC 1 cut(s) 1427
EcoRII CCWGG 5 cut(s) 661, 1813, 1886, 1920, 1969
EcoT14I CCWWGG 1 cut(s) 222
EcoT22I ATGCAT 2 cut(s) 1010, 2284
ErhI CCWWGG 1 cut(s) 222
Esp3I CGTCTC 2 cut(s) 264, 2132
FalI AAGNNNNNCTT 2 cut(s) 1320, 1352
FaqI GGGAC 2 cut(s) 392, 1067
FauI CCCGC 3 cut(s) 175, 197, 1092
FbaI TGATCA 3 cut(s) 1108, 2049, 2095
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 9 cut(s) 91, 252, 285, 581, 1731, 1954, 2187, 2271, 2397
Fsp4HI GCNGC 9 cut(s) 91, 252, 285, 581, 1731, 1954, 2187, 2271, 2397
FspBI CTAG 3 cut(s) 780, 897, 1404
GluI GCNGC 9 cut(s) 91, 252, 285, 581, 1731, 1954, 2187, 2271, 2397
HaeIII GGCC 4 cut(s) 227, 580, 1524, 2235
HapII CCGG 8 cut(s) 248, 326, 508, 846, 1223, 2236, 2460, 2484
HgaI GACGC 1 cut(s) 812
HindIII AAGCTT 4 cut(s) 354, 1140, 1538, 1751
HinfI GANTC 4 cut(s) 184, 443, 1265, 2386
HpaII CCGG 8 cut(s) 248, 326, 508, 846, 1223, 2236, 2460, 2484
HphI GGTGA 5 cut(s) 126, 591, 700, 974, 988
Hpy166II GTNNAC 3 cut(s) 505, 538, 1027
Hpy8I GTNNAC 3 cut(s) 505, 538, 1027
Hpy99I CGWCG 2 cut(s) 1763, 2455
HpyCH4III ACNGT 3 cut(s) 1259, 1394, 2305
HpyCH4IV ACGT 2 cut(s) 381, 413
HpyF10VI GCNNNNNNNGC 9 cut(s) 802, 1084, 1250, 1259, 1619, 1727, 2279, 2361, 2378
HpyF3I CTNAG 5 cut(s) 584, 680, 834, 1202, 1618
HpySE526I ACGT 2 cut(s) 381, 413
Kpn2I TCCGGA 2 cut(s) 845, 2483
Ksp22I TGATCA 3 cut(s) 1108, 2049, 2095
LmnI GCTCC 4 cut(s) 219, 734, 1295, 2449
Lsp1109I GCAGC 4 cut(s) 238, 1717, 2198, 2383
LweI GCATC 6 cut(s) 685, 1240, 1336, 2078, 2101, 2291
MaeI CTAG 3 cut(s) 780, 897, 1404
MaeII ACGT 2 cut(s) 381, 413
MaeIII GTNAC 1 cut(s) 1394
MflI RGATCY 3 cut(s) 878, 1858, 2462
MhlI GDGCHC 2 cut(s) 294, 2446
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 3 cut(s) 131, 921, 1836
Mph1103I ATGCAT 2 cut(s) 1010, 2284
MroI TCCGGA 2 cut(s) 845, 2483
MroXI GAANNNNTTC 1 cut(s) 1932
MseI TTAA 6 cut(s) 669, 726, 1359, 1674, 1962, 2147
MslI CAYNNNNRTG 2 cut(s) 1578, 2159
MspA1I CMGCKG 3 cut(s) 583, 754, 1244
MspI CCGG 8 cut(s) 248, 326, 508, 846, 1223, 2236, 2460, 2484
MspR9I CCNGG 9 cut(s) 248, 326, 509, 663, 1815, 1888, 1922, 1971, 2460
Mva1269I GAATGC 1 cut(s) 2242
MvaI CCWGG 5 cut(s) 663, 1815, 1888, 1922, 1971
MwoI GCNNNNNNNGC 9 cut(s) 802, 1084, 1250, 1259, 1619, 1727, 2279, 2361, 2378
NciI CCSGG 4 cut(s) 248, 326, 509, 2460
NcoI CCATGG 1 cut(s) 222
NlaIV GGNNCC 6 cut(s) 6, 880, 1297, 1479, 2457, 2464
NmeAIII GCCGAG 2 cut(s) 253, 313
NmuCI GTSAC 1 cut(s) 1394
NsiI ATGCAT 2 cut(s) 1010, 2284
NspI RCATGY 5 cut(s) 1082, 1714, 2197, 2266, 2282
PcsI WCGNNNNNNNCGW 3 cut(s) 225, 447, 1558
PctI GAATGC 1 cut(s) 2242
PdmI GAANNNNTTC 1 cut(s) 1932
PfeI GAWTC 3 cut(s) 184, 1265, 2386
PkrI GCNGC 9 cut(s) 92, 253, 286, 582, 1732, 1955, 2188, 2272, 2398
PleI GAGTC 1 cut(s) 451
PmaCI CACGTG 1 cut(s) 414
PmlI CACGTG 1 cut(s) 414
PpsI GAGTC 1 cut(s) 451
Ppu21I YACGTR 1 cut(s) 414
Psp6I CCWGG 5 cut(s) 661, 1813, 1886, 1920, 1969
PspCI CACGTG 1 cut(s) 414
PspGI CCWGG 5 cut(s) 661, 1813, 1886, 1920, 1969
PspN4I GGNNCC 6 cut(s) 6, 880, 1297, 1479, 2457, 2464
PspPI GGNCC 5 cut(s) 505, 1220, 1522, 1799, 2455
PsrI GAACNNNNNNTAC 2 cut(s) 857, 889
PstI CTGCAG 1 cut(s) 2251
PstNI CAGNNNCTG 1 cut(s) 559
PsuI RGATCY 3 cut(s) 878, 1858, 2462
PvuII CAGCTG 1 cut(s) 754
RsaI GTAC 4 cut(s) 552, 1551, 1709, 2169
RsaNI GTAC 4 cut(s) 551, 1550, 1708, 2168
RseI CAYNNNNRTG 2 cut(s) 1578, 2159
SaqAI TTAA 6 cut(s) 669, 726, 1359, 1674, 1962, 2147
SatI GCNGC 9 cut(s) 91, 252, 285, 581, 1731, 1954, 2187, 2271, 2397
Sau96I GGNCC 5 cut(s) 505, 1220, 1522, 1799, 2455
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 9 cut(s) 248, 326, 509, 663, 1815, 1888, 1922, 1971, 2460
SduI GDGCHC 2 cut(s) 294, 2446
SfaNI GCATC 6 cut(s) 685, 1240, 1336, 2078, 2101, 2291
SfcI CTRYAG 2 cut(s) 1778, 2247
SinI GGWCC 4 cut(s) 505, 1220, 1799, 2455
SmiMI CAYNNNNRTG 2 cut(s) 1578, 2159
SmlI CTYRAG 2 cut(s) 1646, 2468
SmoI CTYRAG 2 cut(s) 1646, 2468
SspI AATATT 1 cut(s) 1987
SspMI CTAG 3 cut(s) 780, 897, 1404
StyD4I CCNGG 9 cut(s) 246, 324, 507, 661, 1813, 1886, 1920, 1969, 2458
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 3 cut(s) 1259, 1394, 2305
TaiI ACGT 2 cut(s) 384, 416
TaqI TCGA 7 cut(s) 239, 370, 441, 450, 1561, 1774, 2316
TatI WGTACW 2 cut(s) 550, 1707
TauI GCSGC 5 cut(s) 93, 287, 583, 1956, 2273
TfiI GAWTC 3 cut(s) 184, 1265, 2386
Tru1I TTAA 6 cut(s) 669, 726, 1359, 1674, 1962, 2147
Tru9I TTAA 6 cut(s) 669, 726, 1359, 1674, 1962, 2147
TscAI CASTG 3 cut(s) 186, 1264, 2446
TseFI GTSAC 1 cut(s) 1394
TseI GCWGC 4 cut(s) 251, 1730, 2186, 2396
Tsp45I GTSAC 1 cut(s) 1394
TspDTI ATGAA 6 cut(s) 471, 873, 1161, 1440, 1668, 1941
TspGWI ACGGA 2 cut(s) 330, 2468
TspRI CASTG 3 cut(s) 186, 1264, 2446
VpaK11BI GGWCC 4 cut(s) 505, 1220, 1799, 2455
XapI RAATTY 5 cut(s) 1427, 1484, 1641, 1670, 1868
XbaI TCTAGA 1 cut(s) 1403
XceI RCATGY 5 cut(s) 1082, 1714, 2197, 2266, 2282
XcmI CCANNNNNNNNNTGG 1 cut(s) 1034
XmiI GTMKAC 1 cut(s) 537
XmnI GAANNNNTTC 1 cut(s) 1932
XspI CTAG 3 cut(s) 780, 897, 1404
Zsp2I ATGCAT 2 cut(s) 1010, 2284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.