Rh6DG344800

Spermatogenesis-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0012290
Physical Location & Seq
Forward (+)
54765873 .. 54770912
5040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG344800.1

Sequence Viewer

Length: 2274 bp
ATGGCTCACCAACACACTTCGAACCCAACTTCCCACAAGCACACCAATCGCCTCGCTGCCGAGCACAGTCCCTATCTCCTCCAACACGCCCACAACCCGGTTGATTGGTATCCATGGAGTGAAGAGGCTTTCGCCGAAGCTCGAAAGAGAGATGTGCCGATCTTCTTATCAATCGGGTACAGCACGTGCCATTGGTGTCACGTTATGGAGGTCGAGTCTTTTGAGGATGAAGGCGTTGCCACGTTGCTGAATGAGTGGTTTGTTAGTATCAAGGTGGATCGGGAGGAAAGACCAGATGTGGATAAGGTATACATGACCTATGTACAGGCTCTGTATGGTGGTGGAGGCTGGCCACTGACGGTGTTCCTTTCACCAGATTTGAAACCTTTGATGGGTGGAACTTACTTTCCCCCAGAGGACAAGTATGGAAGACCAGGATTTAAGACTATACTTGGAAAAGTGAAAGAAGCCTGGGAGACCAAGAGGGATGTGCTTGTCAAGAGTGGAGCCTTTGCAATTGAACAGTTGTCAGAAGCATTGTCAACAAGTGCAGGTTCTAAAAAATTGTCAGATGGGATCCCAAAGAATGCTTTGCGTCTATGTGCGGAGCAACTTTCTCAAAGTTATGATTCAAACTTTGGTGGGTTTGGATCTGCCCCAAAGTTTCCTAGACCAGTTGAGATTCAGCTGATGCTTTATTACTCAAAGAAGTCGGAGGAAACTGGAAATTCTGGCAAGGCCAATGAAGTTCTGAAAAAGGTTCTGTTTACCTTGCAATGCATGGCAAAAGGGGGAATTCACGATCACGTCGGAGGTGGCTTCCACCGATATAGCGTGGATGAGTGCTGGCATGTACCGCACTTTGAGAAGATGTTGTATGATCAGGGCCAACTTGCTAATGTCTACCTAGATGCCTTTTCTATTACCAAAGATGTATTCTATGCATCTATTGCTCGGGATGTTCTTGATTATCTGAGGAGAGACATGATTGGACCAGATGGCGAAATATATTCAGCAGAAGATGCTGATAGTGCTGAATCTGAAGGTGCATCCAGAAAAAAGGAAGGAGCATTCTATGTTTGGTCTAGCAAAGAGGTTGAAGACATACTTGGAGAAGATGCAACTCTTTTTAAGAACCACTACTATATAAAGCCTTCCGGTAACTGTGACCTTTCCAGAATGAGTGATCCTCATAATGAATTCAAGAGTAAGAATGTGCTGATTGAGAGAAAAACTCATTCCGAGGCATCAAAATTTTCCATGCCGGTTGAGAAATACCTCAATATTCTGGGTCAGAGCAGGCGAAAGCTTTTTGAAATAAGATGTAGTCGACCAAGACCACATTTGGATGATAAGGTAATTGTAGCATGGAATGGACTGGCCATCTCAGCCTTTGCAAGAGCCTCTAAAATTCTAAAGAATGAACTTAAGGAGATCAAGTTCAACTTTCCTGTTGTTGGTTGTGATCCGCGGGAGTACATTGAAGTTGCAGAAAAGGCAGCATCTTTTATCAGGCGACACCTTTACAATGAGCAATCTCATCGACTGCAACGCAGCTTTAGGAACGGCCCATCTAAAGCGCCTGGATTTTTAGATGATTATGCTTTTCTTATTTCAGGGTTATTGGATCTTTATGAATTTGGTGGCCAAACCAGCTGGCTAGTTTGGGCAATTGAACTGCAAGACATGCAGGATGAAATATTTCTTGATAAAGAGGGAGGCGGCTATTTTAATACCCCAGGTGATGACCCTAGTGTTCTCCTTCGTGTGAAGGAAGATCATGATGGGGCAGAGCCCTCTGGAAACTCGGTTTCTGTGATCAACCTTGTGAGATTGGCATCAATACTATCTGGCAACAGGTCTGAGCATTACAGACAGGAAGCTGAACATGTTCTGGCGGTTTTTGAGACAAGATTAAACGACATGGCCATGGCAGTACCTTTAATGTGTTGTGCTGCAGACATGCTCACTGTTCCTTCTCGGAAGCAAGTTGTCTTGGTTGGCCAGAAAACTAGAGAGTTTGAAAACATGCTTGCCGCAGCTCATGCATCATATGATACCAACAAAACGGTTATTCACATAGATCCAACTAATTCGGAAGAGTTGCAATTCTGGGAAGACAAAAACAGCAACATTGCGCTCATGGCAAAGAATCATGTTGCTGCAGACAAAGTGGTTGCTCTCGTTTGCCAAAACTTTACTTGCAGTGCGCCTGTCGCTGACCCTGGATCCCTGGAGGCTCTGCTCTCCAAGAAATCAGTCCCTTCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
Thioredox_DsbH

Protein Analysis

757

Amino Acids

85.01

Weight (kDa)

5.84

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredox_DsbH PF03190 15 - 175 7.9e-76 Protein of unknown function, DUF255
Thioredoxin_7 PF13899 35 - 125 1.6e-09 Thioredoxin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012290)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03200 AT4G03200
fragaria_vesca FvH4_2g25030
malus_domestica MD03G1280600.v1.1
prunus_persica Prupe.8G255100_v2.0.a1
pyrus_communis pycom03g22230
rosa_chinensis RchiOBHm_Chr6g0293001
rosa_laevigata RLG00000011975
rosa_multiflora Rmu_sc0001788.1_g000005 Rmu_sc0003615.1_g000002 Rmu_sc0028741.1_g000001
rosa_roxburghii Rroxscaffold_7G00174480
rosa_rugosa Rorug06G0231900 Rorug06G0232000
rosa_samantha Rh6AG344700 Rh6BG352100 Rh6CG359300 Rh6DG344800
rosa_wichuraiana Rw6G030080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 542
AccI GTMKAC 3 cut(s) 309, 903, 1330
AccII CGCG 1 cut(s) 1471
AciI CCGC 7 cut(s) 605, 857, 1469, 1471, 1722, 1898, 2037
AcoI YGGCCR 5 cut(s) 350, 1380, 1645, 1926, 2002
AcsI RAATTY 6 cut(s) 727, 795, 1199, 1253, 1410, 1637
AcuI CTGAAG 2 cut(s) 1062, 2250
AcvI CACGTG 1 cut(s) 186
AfaI GTAC 5 cut(s) 179, 324, 855, 1478, 1938
AfiI CCNNNNNNNGG 3 cut(s) 97, 392, 1457
AflII CTTAAG 1 cut(s) 1427
AflIII ACRYGT 1 cut(s) 1888
AjiI CACGTC 1 cut(s) 808
AjnI CCWGG 6 cut(s) 433, 470, 1582, 1738, 2224, 2232
AjuI GAANNNNNNNTTGG 4 cut(s) 920, 952, 1092, 1124
AloI GAACNNNNNNTCC 6 cut(s) 14, 46, 391, 423, 1424, 1456
AluBI AGCT 8 cut(s) 140, 688, 1309, 1557, 1656, 1883, 2042, 2270
AluI AGCT 8 cut(s) 140, 688, 1309, 1557, 1656, 1883, 2042, 2270
Alw21I GWGCWC 1 cut(s) 66
Alw26I GTCTC 3 cut(s) 470, 975, 1901
AlwNI CAGNNNCTG 1 cut(s) 331
Ama87I CYCGRG 1 cut(s) 954
AoxI GGCC 8 cut(s) 350, 738, 886, 1380, 1567, 1645, 1926, 2002
ApeKI GCWGC 6 cut(s) 56, 1499, 1554, 1955, 2039, 2162
ApoI RAATTY 6 cut(s) 727, 795, 1199, 1253, 1410, 1637
Asp700I GAANNNNTTC 2 cut(s) 1701, 1890
AspLEI GCGC 3 cut(s) 1582, 2140, 2212
AspS9I GGNCC 3 cut(s) 886, 992, 1568
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 2 cut(s) 363, 1754
AsuII TTCGAA 1 cut(s) 20
AvaI CYCGRG 1 cut(s) 954
AvaII GGWCC 1 cut(s) 992
BalI TGGCCA 5 cut(s) 352, 1382, 1647, 1928, 2004
BamHI GGATCC 2 cut(s) 576, 2228
BanII GRGCYC 1 cut(s) 1797
BbrPI CACGTG 1 cut(s) 186
BbsI GAAGAC 3 cut(s) 436, 1107, 2124
Bbv12I GWGCWC 1 cut(s) 66
BbvI GCAGC 6 cut(s) 43, 1511, 1566, 1942, 2051, 2149
BccI CCATC 6 cut(s) 385, 566, 992, 1391, 1579, 1778
BceAI ACGGC 1 cut(s) 1582
BcgI CGANNNNNNTGC 4 cut(s) 29, 63, 1523, 1557
BciT130I CCWGG 6 cut(s) 435, 472, 1584, 1740, 2226, 2234
BciVI GTATCC 1 cut(s) 120
BclI TGATCA 2 cut(s) 880, 1818
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 3 cut(s) 470, 975, 1901
BfaI CTAG 6 cut(s) 669, 908, 1086, 1661, 1752, 2013
BfmI CTRYAG 2 cut(s) 1956, 2163
BfoI RGCGCY 1 cut(s) 1583
BfrI CTTAAG 1 cut(s) 1427
BfuAI ACCTGC 1 cut(s) 542
BfuI GTATCC 1 cut(s) 120
BisI GCNGC 8 cut(s) 57, 1500, 1555, 1723, 1956, 2037, 2040, 2163
BlsI GCNGC 8 cut(s) 58, 1501, 1556, 1724, 1957, 2038, 2041, 2164
Bme1390I CCNGG 7 cut(s) 98, 435, 472, 1584, 1740, 2226, 2234
Bme18I GGWCC 1 cut(s) 992
BmeT110I CYCGRG 1 cut(s) 954
BmgBI CACGTC 1 cut(s) 808
BmgT120I GGNCC 3 cut(s) 886, 992, 1568
BmiI GGNNCC 3 cut(s) 508, 578, 2230
BmrFI CCNGG 7 cut(s) 98, 435, 472, 1584, 1740, 2226, 2234
BpiI GAAGAC 3 cut(s) 436, 1107, 2124
BplI GAGNNNNNCTC 4 cut(s) 1174, 1206, 1219, 1251
BpmI CTGGAG 1 cut(s) 2255
Bpu14I TTCGAA 1 cut(s) 20
BpuMI CCSGG 1 cut(s) 98
BsaAI YACGTR 1 cut(s) 186
BsaBI GATNNNNATC 2 cut(s) 108, 1837
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 8 cut(s) 113, 471, 1242, 1469, 1738, 1929, 2224, 2232
BsaWI WCCGGW 1 cut(s) 1157
BsaXI ACNNNNNCTCC 4 cut(s) 200, 230, 1424, 1454
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 392, 1457
Bse118I RCCGGY 1 cut(s) 1264
Bse1I ACTGG 3 cut(s) 674, 727, 1383
Bse3DI GCAATG 2 cut(s) 782, 2133
Bse8I GATNNNNATC 2 cut(s) 108, 1837
BseBI CCWGG 6 cut(s) 435, 472, 1584, 1740, 2226, 2234
BseDI CCNNGG 8 cut(s) 113, 471, 1242, 1469, 1738, 1929, 2224, 2232
BseGI GGATG 7 cut(s) 232, 493, 844, 964, 1049, 1354, 1699
BseJI GATNNNNATC 2 cut(s) 108, 1837
BseLI CCNNNNNNNGG 3 cut(s) 97, 392, 1457
BseMI GCAATG 2 cut(s) 782, 2133
BseMII CTCAG 3 cut(s) 965, 1401, 1854
BseNI ACTGG 3 cut(s) 674, 727, 1383
BseRI GAGGAG 2 cut(s) 68, 991
BseXI GCAGC 6 cut(s) 43, 1511, 1566, 1942, 2051, 2149
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 1 cut(s) 1471
BshFI GGCC 8 cut(s) 352, 740, 888, 1382, 1569, 1647, 1928, 2004
BsiHKAI GWGCWC 1 cut(s) 66
BsiHKCI CYCGRG 1 cut(s) 954
BsiSI CCGG 3 cut(s) 98, 1158, 1265
BslFI GGGAC 2 cut(s) 54, 2246
BslI CCNNNNNNNGG 3 cut(s) 97, 392, 1457
BsmAI GTCTC 3 cut(s) 470, 975, 1901
BsmFI GGGAC 2 cut(s) 54, 2246
BsmI GAATGC 2 cut(s) 592, 1070
BsnI GGCC 8 cut(s) 352, 740, 888, 1382, 1569, 1647, 1928, 2004
Bso31I GGTCTC 1 cut(s) 470
BsoBI CYCGRG 1 cut(s) 954
Bsp119I TTCGAA 1 cut(s) 20
Bsp1286I GDGCHC 2 cut(s) 66, 1797
Bsp1407I TGTACA 1 cut(s) 322
Bsp19I CCATGG 2 cut(s) 113, 1929
BspACI CCGC 7 cut(s) 605, 857, 1469, 1471, 1722, 1898, 2037
BspANI GGCC 8 cut(s) 352, 740, 888, 1382, 1569, 1647, 1928, 2004
BspCNI CTCAG 3 cut(s) 966, 1400, 1855
BspFNI CGCG 1 cut(s) 1471
BspHI TCATGA 1 cut(s) 1780
BspLI GGNNCC 3 cut(s) 508, 578, 2230
BspMAI CTGCAG 2 cut(s) 1960, 2167
BspMI ACCTGC 1 cut(s) 542
BspT104I TTCGAA 1 cut(s) 20
BspTI CTTAAG 1 cut(s) 1427
BspTNI GGTCTC 1 cut(s) 470
BsrDI GCAATG 2 cut(s) 782, 2133
BsrFI RCCGGY 1 cut(s) 1264
BsrGI TGTACA 1 cut(s) 322
BsrI ACTGG 3 cut(s) 674, 727, 1383
BssAI RCCGGY 1 cut(s) 1264
BssECI CCNNGG 8 cut(s) 113, 471, 1242, 1469, 1738, 1929, 2224, 2232
BssNAI GTATAC 1 cut(s) 310
BssT1I CCWWGG 2 cut(s) 113, 1929
Bst1107I GTATAC 1 cut(s) 310
Bst2UI CCWGG 6 cut(s) 435, 472, 1584, 1740, 2226, 2234
Bst4CI ACNGT 6 cut(s) 68, 361, 525, 1166, 1972, 2071
Bst6I CTCTTC 2 cut(s) 117, 2094
BstAFI CTTAAG 1 cut(s) 1427
BstAPI GCANNNNNTGC 4 cut(s) 950, 1022, 1687, 2045
BstAUI TGTACA 1 cut(s) 322
BstBAI YACGTR 1 cut(s) 186
BstBI TTCGAA 1 cut(s) 20
BstC8I GCNNGC 5 cut(s) 350, 848, 1301, 1658, 2034
BstDEI CTNAG 3 cut(s) 974, 1387, 1863
BstDSI CCRYGG 3 cut(s) 113, 1469, 1929
BstF5I GGATG 7 cut(s) 232, 493, 844, 964, 1049, 1354, 1699
BstFNI CGCG 1 cut(s) 1471
BstH2I RGCGCY 1 cut(s) 1583
BstHHI GCGC 3 cut(s) 1582, 2140, 2212
BstMAI GTCTC 3 cut(s) 470, 975, 1901
BstNI CCWGG 6 cut(s) 435, 472, 1584, 1740, 2226, 2234
BstNSI RCATGY 5 cut(s) 854, 1690, 1892, 1966, 2032
BstSCI CCNGG 7 cut(s) 96, 433, 470, 1582, 1738, 2224, 2232
BstSFI CTRYAG 2 cut(s) 1956, 2163
BstUI CGCG 1 cut(s) 1471
BstV1I GCAGC 6 cut(s) 43, 1511, 1566, 1942, 2051, 2149
BstV2I GAAGAC 3 cut(s) 436, 1107, 2124
BstX2I RGATCY 5 cut(s) 576, 650, 1627, 2083, 2228
BstYI RGATCY 5 cut(s) 576, 650, 1627, 2083, 2228
BstZ17I GTATAC 1 cut(s) 310
BsuI GTATCC 1 cut(s) 120
BsuRI GGCC 8 cut(s) 352, 740, 888, 1382, 1569, 1647, 1928, 2004
BtgI CCRYGG 3 cut(s) 113, 1469, 1929
BtrI CACGTC 1 cut(s) 808
BtsCI GGATG 7 cut(s) 232, 493, 844, 964, 1049, 1354, 1699
BtsI GCAGTG 1 cut(s) 2212
BtsIMutI CAGTG 3 cut(s) 353, 1968, 2212
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 5 cut(s) 350, 848, 1301, 1658, 2034
CaiI CAGNNNCTG 1 cut(s) 331
CciI TCATGA 1 cut(s) 1780
CfoI GCGC 3 cut(s) 1582, 2140, 2212
Cfr10I RCCGGY 1 cut(s) 1264
Cfr13I GGNCC 3 cut(s) 886, 992, 1568
Cfr42I CCGCGG 1 cut(s) 1472
CseI GACGC 1 cut(s) 584
Csp6I GTAC 5 cut(s) 178, 323, 854, 1477, 1937
CviQI GTAC 5 cut(s) 178, 323, 854, 1477, 1937
DdeI CTNAG 3 cut(s) 974, 1387, 1863
EaeI YGGCCR 5 cut(s) 350, 1380, 1645, 1926, 2002
Eam1104I CTCTTC 2 cut(s) 117, 2094
EarI CTCTTC 2 cut(s) 117, 2094
Eco130I CCWWGG 2 cut(s) 113, 1929
Eco24I GRGCYC 1 cut(s) 1797
Eco31I GGTCTC 1 cut(s) 470
Eco47I GGWCC 1 cut(s) 992
Eco57I CTGAAG 2 cut(s) 1062, 2250
Eco72I CACGTG 1 cut(s) 186
Eco88I CYCGRG 1 cut(s) 954
EcoRI GAATTC 2 cut(s) 795, 1199
EcoRII CCWGG 6 cut(s) 433, 470, 1582, 1738, 2224, 2232
EcoT14I CCWWGG 2 cut(s) 113, 1929
EcoT22I ATGCAT 3 cut(s) 782, 946, 2050
EcoT38I GRGCYC 1 cut(s) 1797
ErhI CCWWGG 2 cut(s) 113, 1929
FalI AAGNNNNNCTT 4 cut(s) 1092, 1124, 1430, 1462
FaqI GGGAC 2 cut(s) 54, 2246
FauI CCCGC 1 cut(s) 1464
FauNDI CATATG 1 cut(s) 2053
FbaI TGATCA 2 cut(s) 880, 1818
FblI GTMKAC 3 cut(s) 309, 903, 1330
Fnu4HI GCNGC 8 cut(s) 57, 1500, 1555, 1723, 1956, 2037, 2040, 2163
FokI GGATG 7 cut(s) 239, 500, 851, 971, 1036, 1361, 1706
FriOI GRGCYC 1 cut(s) 1797
Fsp4HI GCNGC 8 cut(s) 57, 1500, 1555, 1723, 1956, 2037, 2040, 2163
FspBI CTAG 6 cut(s) 669, 908, 1086, 1661, 1752, 2013
GlaI GCGC 3 cut(s) 1581, 2139, 2211
GluI GCNGC 8 cut(s) 57, 1500, 1555, 1723, 1956, 2037, 2040, 2163
GsuI CTGGAG 1 cut(s) 2255
HaeII RGCGCY 1 cut(s) 1583
HaeIII GGCC 8 cut(s) 352, 740, 888, 1382, 1569, 1647, 1928, 2004
HapII CCGG 3 cut(s) 98, 1158, 1265
HgaI GACGC 1 cut(s) 584
HhaI GCGC 3 cut(s) 1582, 2140, 2212
Hin6I GCGC 3 cut(s) 1580, 2138, 2210
HinP1I GCGC 3 cut(s) 1580, 2138, 2210
HincII GTYRAC 2 cut(s) 543, 1331
HindII GTYRAC 2 cut(s) 543, 1331
HindIII AAGCTT 1 cut(s) 1307
HinfI GANTC 5 cut(s) 215, 629, 682, 1037, 2152
HpaII CCGG 3 cut(s) 98, 1158, 1265
HphI GGTGA 2 cut(s) 363, 1754
Hpy166II GTNNAC 5 cut(s) 310, 543, 768, 904, 1331
Hpy8I GTNNAC 5 cut(s) 310, 543, 768, 904, 1331
Hpy99I CGWCG 1 cut(s) 812
HpyAV CCTTC 8 cut(s) 224, 1037, 1058, 1164, 1765, 1772, 1986, 2274
HpyCH4III ACNGT 6 cut(s) 68, 361, 525, 1166, 1972, 2071
HpyCH4IV ACGT 4 cut(s) 185, 201, 242, 807
HpyF3I CTNAG 3 cut(s) 974, 1387, 1863
HpySE526I ACGT 4 cut(s) 185, 201, 242, 807
HspAI GCGC 3 cut(s) 1580, 2138, 2210
Ksp22I TGATCA 2 cut(s) 880, 1818
KspI CCGCGG 1 cut(s) 1472
LmnI GCTCC 3 cut(s) 506, 607, 1067
Lsp1109I GCAGC 6 cut(s) 43, 1511, 1566, 1942, 2051, 2149
MaeI CTAG 6 cut(s) 669, 908, 1086, 1661, 1752, 2013
MaeII ACGT 4 cut(s) 185, 201, 242, 807
MaeIII GTNAC 3 cut(s) 197, 1160, 1166
MfeI CAATTG 2 cut(s) 516, 1671
MflI RGATCY 5 cut(s) 576, 650, 1627, 2083, 2228
MhlI GDGCHC 2 cut(s) 66, 1797
MlsI TGGCCA 5 cut(s) 352, 1382, 1647, 1928, 2004
MluNI TGGCCA 5 cut(s) 352, 1382, 1647, 1928, 2004
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 4 cut(s) 106, 693, 790, 2111
Mox20I TGGCCA 5 cut(s) 352, 1382, 1647, 1928, 2004
Mph1103I ATGCAT 3 cut(s) 782, 946, 2050
MroXI GAANNNNTTC 2 cut(s) 1701, 1890
MscI TGGCCA 5 cut(s) 352, 1382, 1647, 1928, 2004
MseI TTAA 6 cut(s) 441, 1131, 1428, 1731, 1916, 1943
MslI CAYNNNNRTG 2 cut(s) 1347, 1928
Msp20I TGGCCA 5 cut(s) 352, 1382, 1647, 1928, 2004
MspA1I CMGCKG 3 cut(s) 688, 1471, 1656
MspCI CTTAAG 1 cut(s) 1427
MspI CCGG 3 cut(s) 98, 1158, 1265
MspR9I CCNGG 7 cut(s) 98, 435, 472, 1584, 1740, 2226, 2234
MunI CAATTG 2 cut(s) 516, 1671
Mva1269I GAATGC 2 cut(s) 592, 1070
MvaI CCWGG 6 cut(s) 435, 472, 1584, 1740, 2226, 2234
MvnI CGCG 1 cut(s) 1471
NciI CCSGG 1 cut(s) 98
NcoI CCATGG 2 cut(s) 113, 1929
NdeI CATATG 1 cut(s) 2053
NlaIV GGNNCC 3 cut(s) 508, 578, 2230
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 197, 1166
NsiI ATGCAT 3 cut(s) 782, 946, 2050
NspI RCATGY 5 cut(s) 854, 1690, 1892, 1966, 2032
NspV TTCGAA 1 cut(s) 20
PagI TCATGA 1 cut(s) 1780
PciI ACATGT 1 cut(s) 1888
PctI GAATGC 2 cut(s) 592, 1070
PdmI GAANNNNTTC 2 cut(s) 1701, 1890
PfeI GAWTC 4 cut(s) 629, 682, 1037, 2152
PkrI GCNGC 8 cut(s) 58, 1501, 1556, 1724, 1957, 2038, 2041, 2164
PleI GAGTC 1 cut(s) 223
PmaCI CACGTG 1 cut(s) 186
PmlI CACGTG 1 cut(s) 186
PpsI GAGTC 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 186
PscI ACATGT 1 cut(s) 1888
Psp6I CCWGG 6 cut(s) 433, 470, 1582, 1738, 2224, 2232
PspCI CACGTG 1 cut(s) 186
PspGI CCWGG 6 cut(s) 433, 470, 1582, 1738, 2224, 2232
PspN4I GGNNCC 3 cut(s) 508, 578, 2230
PspPI GGNCC 3 cut(s) 886, 992, 1568
PstI CTGCAG 2 cut(s) 1960, 2167
PstNI CAGNNNCTG 1 cut(s) 331
PsuI RGATCY 5 cut(s) 576, 650, 1627, 2083, 2228
PvuII CAGCTG 2 cut(s) 688, 1656
RsaI GTAC 5 cut(s) 179, 324, 855, 1478, 1938
RsaNI GTAC 5 cut(s) 178, 323, 854, 1477, 1937
RseI CAYNNNNRTG 2 cut(s) 1347, 1928
SacII CCGCGG 1 cut(s) 1472
SalI GTCGAC 1 cut(s) 1329
SaqAI TTAA 6 cut(s) 441, 1131, 1428, 1731, 1916, 1943
SatI GCNGC 8 cut(s) 57, 1500, 1555, 1723, 1956, 2037, 2040, 2163
Sau96I GGNCC 3 cut(s) 886, 992, 1568
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 7 cut(s) 98, 435, 472, 1584, 1740, 2226, 2234
SduI GDGCHC 2 cut(s) 66, 1797
SfcI CTRYAG 2 cut(s) 1956, 2163
Sfr303I CCGCGG 1 cut(s) 1472
SfuI TTCGAA 1 cut(s) 20
SgrBI CCGCGG 1 cut(s) 1472
SinI GGWCC 1 cut(s) 992
SmiMI CAYNNNNRTG 2 cut(s) 1347, 1928
SmlI CTYRAG 1 cut(s) 1427
SmoI CTYRAG 1 cut(s) 1427
SsiI CCGC 7 cut(s) 605, 857, 1469, 1471, 1722, 1898, 2037
SspI AATATT 2 cut(s) 1285, 1701
SspMI CTAG 6 cut(s) 669, 908, 1086, 1661, 1752, 2013
StyD4I CCNGG 7 cut(s) 96, 433, 470, 1582, 1738, 2224, 2232
StyI CCWWGG 2 cut(s) 113, 1929
TaaI ACNGT 6 cut(s) 68, 361, 525, 1166, 1972, 2071
TaiI ACGT 4 cut(s) 188, 204, 245, 810
TaqI TCGA 5 cut(s) 20, 142, 213, 1330, 1543
TatI WGTACW 2 cut(s) 322, 1476
TauI GCSGC 2 cut(s) 1725, 2039
TfiI GAWTC 4 cut(s) 629, 682, 1037, 2152
Tru1I TTAA 6 cut(s) 441, 1131, 1428, 1731, 1916, 1943
Tru9I TTAA 6 cut(s) 441, 1131, 1428, 1731, 1916, 1943
TscAI CASTG 3 cut(s) 360, 1975, 2212
TseFI GTSAC 2 cut(s) 197, 1166
TseI GCWGC 6 cut(s) 56, 1499, 1554, 1955, 2039, 2162
Tsp45I GTSAC 2 cut(s) 197, 1166
TspDTI ATGAA 6 cut(s) 243, 759, 1212, 1437, 1650, 1710
TspRI CASTG 3 cut(s) 360, 1975, 2212
Vha464I CTTAAG 1 cut(s) 1427
VpaK11BI GGWCC 1 cut(s) 992
XapI RAATTY 6 cut(s) 727, 795, 1199, 1253, 1410, 1637
XceI RCATGY 5 cut(s) 854, 1690, 1892, 1966, 2032
XmiI GTMKAC 3 cut(s) 309, 903, 1330
XmnI GAANNNNTTC 2 cut(s) 1701, 1890
XspI CTAG 6 cut(s) 669, 908, 1086, 1661, 1752, 2013
Zsp2I ATGCAT 3 cut(s) 782, 946, 2050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.