Rorug06G0232000

Spermatogenesis-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012290
Physical Location & Seq
Forward (+)
37298932 .. 37300436
1505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0232000.1

Sequence Viewer

Length: 1383 bp
ATGAAGATGGTCGAAGTCAATCTTCGGACGGAGGTCGGAGCTCTAACCGAGGCATTGAAGGACCAGAAGGTTCGGATACGCGGCCGTGCGCAGACGATTAGACCCACCGGGGAGAAGCTGGCGTTTATTGTGGTCAGAGAGAAGGGGTTCACGGTTCAGTGCGTCGTGACGGTTCAGCGGGAGATAGTGAGCTGTCAGATGGTGAAGTACGCGTCGAAGTTGAGCCGCGAGTCGATTATTGACATTGAAGGGGTTGTGTCTAAACCTGATATGGCAATTGCAGGCGCTTCACAGCAGGTGGATGTGCAAGTTAGAAAGTTGTACTGTATAAGTAGGGCTGCTGTTTTACCTATTGAAATTGAGAATGCTGCTCGAAGTGAAAAACCTACTGAGGATGGACAGAATACTCGCTTGAACAACCGAGTACTTGACTTGCGAACAGCGGCCAACCGGGGGATTTTCCGAATCCAGAGTCAAGTTGGAACTATCTTCAGGCAGTTCTTGCTGTCCGAAGGGTTTATTGAAATCCATACACCAAAACTGATCGCTGGTTCAAGTGAAGGTGGGGCTTCGGTGTTTAAGCTGGACTACGTGAAGGGGCAACCTGCCTGCCTTGCCCAGTCACCGCAGCTCCACAAGCAGATGTCCATCTGTGCTGACTTTGGTCGTGTTTTCGAGGTTGGTTCTGTTTTCAGAGCTGAGAACTCTGATACACATAGGCATCTATGTGAGTTCACTGGTCTCGATATTGAGATGGAGATTGAGTGGAGCTATTTTGAGGTTATGGATATTGTTAACAGGTTGTTTGTCAATATGTTTGACTATTTGAACAAAAATTGTGAGAAGGAACTTGAAGCTGTACGAAGGCAGTATCCCTTCGAACCGTTAAAGTACTTGCCTGAGACTCTACGTCTCACTTTTGCGGAAGGTGTTCAAATGCTAAAGGATGCCGGTGTGGAAGTTGATCCTATGGGGGACTTGGATACTAAAAAAGAGAGGAAGTTGGGCCAAATAGTTTTGGAGAAATATGGCACTGAGTTCTACATACTTCACCGCTATCCGTTGGCTGTGAGGCCTTTCTATACCATGCCTTGCGACGATAATCCTGAATACAGTAATTCGTTTGATGTCTTCATTCGAGGTGAGGAGATTATATCAGGTGCACAACGTATCCATGTACCAGAATTATTGGTCGAACGTGCTGAAGCACGTGGAATTGAAGTTGAGACAGTATCAACATATATTGATTCCTTCAGATACGGTGCACCTCCCCATGGTGGATTTGGAGTAGGGCTGGAGCGTGTTGTGATGCTCTTCTGTGGTTTGAATAACATCCGCAAAACATCTTTATTCCCTCGTGATCCAGACCGGCTAGCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
Thioredox_DsbH

Protein Analysis

460

Amino Acids

52.09

Weight (kDa)

6.1

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_2 PF00152 134 - 454 2.2e-79 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012290)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03200 AT4G03200
fragaria_vesca FvH4_2g25030
malus_domestica MD03G1280600.v1.1
prunus_persica Prupe.8G255100_v2.0.a1
pyrus_communis pycom03g22230
rosa_chinensis RchiOBHm_Chr6g0293001
rosa_laevigata RLG00000011975
rosa_multiflora Rmu_sc0001788.1_g000005 Rmu_sc0003615.1_g000002 Rmu_sc0028741.1_g000001
rosa_roxburghii Rroxscaffold_7G00174480
rosa_rugosa Rorug06G0231900 Rorug06G0232000
rosa_samantha Rh6AG344700 Rh6BG352100 Rh6CG359300 Rh6DG344800
rosa_wichuraiana Rw6G030080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 286
Acc16I TGCGCA 1 cut(s) 90
Acc36I ACCTGC 2 cut(s) 286, 613
AccII CGCG 3 cut(s) 81, 212, 228
AciI CCGC 8 cut(s) 81, 178, 226, 443, 626, 923, 1054, 1336
AclWI GGATC 2 cut(s) 959, 1355
AcoI YGGCCR 2 cut(s) 82, 444
AcuI CTGAAG 3 cut(s) 475, 1224, 1237
AcvI CACGTG 1 cut(s) 1211
AfaI GTAC 6 cut(s) 209, 323, 426, 861, 893, 1179
AfiI CCNNNNNNNGG 3 cut(s) 453, 1274, 1277
AflIII ACRYGT 1 cut(s) 210
AhdI GACNNNNNGTC 1 cut(s) 909
AluBI AGCT 9 cut(s) 41, 118, 192, 583, 631, 698, 771, 857, 1376
AluI AGCT 9 cut(s) 41, 118, 192, 583, 631, 698, 771, 857, 1376
Alw21I GWGCWC 3 cut(s) 43, 1165, 1267
Alw26I GTCTC 4 cut(s) 746, 896, 917, 1220
Alw44I GTGCAC 2 cut(s) 1161, 1263
AlwI GGATC 2 cut(s) 959, 1355
AoxI GGCC 4 cut(s) 82, 444, 1006, 1073
ApaLI GTGCAC 2 cut(s) 1161, 1263
ApeKI GCWGC 3 cut(s) 338, 368, 628
ArsI GACNNNNNNTTYG 2 cut(s) 457, 489
Asp700I GAANNNNTTC 2 cut(s) 146, 930
AspLEI GCGC 2 cut(s) 91, 287
AspS9I GGNCC 2 cut(s) 61, 1006
AsuC2I CCSGG 2 cut(s) 109, 452
AsuHPI GGTGA 4 cut(s) 214, 615, 1043, 1154
AsuII TTCGAA 1 cut(s) 879
AsuNHI GCTAGC 1 cut(s) 1372
AvaII GGWCC 1 cut(s) 61
BaeGI GKGCMC 2 cut(s) 1165, 1267
BanII GRGCYC 1 cut(s) 43
BauI CACGAG 1 cut(s) 1356
BbrPI CACGTG 1 cut(s) 1211
BbsI GAAGAC 1 cut(s) 1123
Bbv12I GWGCWC 3 cut(s) 43, 1165, 1267
BbvI GCAGC 3 cut(s) 325, 355, 640
BccI CCATC 4 cut(s) 193, 389, 656, 748
BceAI ACGGC 1 cut(s) 69
BciVI GTATCC 4 cut(s) 69, 882, 976, 1181
BcnI CCSGG 2 cut(s) 109, 452
BcoDI GTCTC 4 cut(s) 746, 896, 917, 1220
BfaI CTAG 1 cut(s) 1373
BfoI RGCGCY 1 cut(s) 288
BfuAI ACCTGC 2 cut(s) 286, 613
BfuI GTATCC 4 cut(s) 69, 882, 976, 1181
BisI GCNGC 6 cut(s) 82, 226, 339, 369, 444, 629
BlsI GCNGC 6 cut(s) 83, 227, 340, 370, 445, 630
BmcAI AGTACT 2 cut(s) 426, 893
Bme1390I CCNGG 2 cut(s) 109, 452
Bme18I GGWCC 1 cut(s) 61
BmeRI GACNNNNNGTC 1 cut(s) 909
BmgT120I GGNCC 2 cut(s) 61, 1006
BmrFI CCNGG 2 cut(s) 109, 452
BmrI ACTGGG 1 cut(s) 613
BmsI GCATC 3 cut(s) 730, 937, 1299
BmtI GCTAGC 1 cut(s) 1376
BmuI ACTGGG 1 cut(s) 613
BoxI GACNNNNGTC 2 cut(s) 32, 663
BpiI GAAGAC 1 cut(s) 1123
BpmI CTGGAG 1 cut(s) 1316
Bpu14I TTCGAA 1 cut(s) 879
BpuMI CCSGG 2 cut(s) 109, 452
BsaAI YACGTR 2 cut(s) 592, 1211
BsaBI GATNNNNATC 1 cut(s) 647
BsaI GGTCTC 1 cut(s) 746
BsaJI CCNNGG 4 cut(s) 48, 108, 451, 1273
BsaXI ACNNNNNCTCC 4 cut(s) 615, 645, 749, 779
Bsc4I CCNNNNNNNGG 3 cut(s) 453, 1274, 1277
Bse118I RCCGGY 2 cut(s) 950, 1368
Bse1I ACTGG 2 cut(s) 619, 742
Bse8I GATNNNNATC 1 cut(s) 647
BseDI CCNNGG 4 cut(s) 48, 108, 451, 1273
BseGI GGATG 4 cut(s) 307, 400, 952, 1332
BseJI GATNNNNATC 1 cut(s) 647
BseLI CCNNNNNNNGG 3 cut(s) 453, 1274, 1277
BseMII CTCAG 4 cut(s) 381, 690, 891, 1026
BseNI ACTGG 2 cut(s) 619, 742
BseRI GAGGAG 1 cut(s) 1160
BseSI GKGCMC 2 cut(s) 1165, 1267
BseX3I CGGCCG 1 cut(s) 82
BseXI GCAGC 3 cut(s) 325, 355, 640
Bsh1236I CGCG 3 cut(s) 81, 212, 228
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 4 cut(s) 84, 446, 1008, 1075
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 3 cut(s) 43, 1165, 1267
BsiSI CCGG 4 cut(s) 108, 451, 951, 1369
BslFI GGGAC 1 cut(s) 989
BslI CCNNNNNNNGG 3 cut(s) 453, 1274, 1277
BsmAI GTCTC 4 cut(s) 746, 896, 917, 1220
BsmBI CGTCTC 1 cut(s) 917
BsmFI GGGAC 1 cut(s) 989
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 4 cut(s) 84, 446, 1008, 1075
Bso31I GGTCTC 1 cut(s) 746
Bsp119I TTCGAA 1 cut(s) 879
Bsp1286I GDGCHC 3 cut(s) 43, 1165, 1267
Bsp143I GATC 3 cut(s) 543, 964, 1360
Bsp19I CCATGG 1 cut(s) 1273
BspACI CCGC 8 cut(s) 81, 178, 226, 443, 626, 923, 1054, 1336
BspANI GGCC 4 cut(s) 84, 446, 1008, 1075
BspCNI CTCAG 4 cut(s) 382, 691, 892, 1027
BspFNI CGCG 3 cut(s) 81, 212, 228
BspMI ACCTGC 2 cut(s) 286, 613
BspOI GCTAGC 1 cut(s) 1376
BspPI GGATC 2 cut(s) 959, 1355
BspQI GCTCTTC 1 cut(s) 1319
BspT104I TTCGAA 1 cut(s) 879
BspTNI GGTCTC 1 cut(s) 746
BsrFI RCCGGY 2 cut(s) 950, 1368
BsrI ACTGG 2 cut(s) 619, 742
BssAI RCCGGY 2 cut(s) 950, 1368
BssECI CCNNGG 4 cut(s) 48, 108, 451, 1273
BssMI GATC 3 cut(s) 543, 964, 1360
BssSI CACGAG 1 cut(s) 1356
BssT1I CCWWGG 1 cut(s) 1273
Bst2BI CACGAG 1 cut(s) 1356
Bst4CI ACNGT 7 cut(s) 154, 172, 326, 885, 1115, 1231, 1262
Bst6I CTCTTC 1 cut(s) 1319
BstAPI GCANNNNNTGC 1 cut(s) 502
BstBAI YACGTR 2 cut(s) 592, 1211
BstBI TTCGAA 1 cut(s) 879
BstC8I GCNNGC 4 cut(s) 120, 283, 610, 1374
BstDEI CTNAG 4 cut(s) 390, 699, 900, 1035
BstDSI CCRYGG 1 cut(s) 1273
BstF5I GGATG 4 cut(s) 307, 400, 952, 1332
BstFNI CGCG 3 cut(s) 81, 212, 228
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 2 cut(s) 91, 287
BstKTI GATC 3 cut(s) 546, 967, 1363
BstMAI GTCTC 4 cut(s) 746, 896, 917, 1220
BstMBI GATC 3 cut(s) 543, 964, 1360
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 3 cut(s) 502, 614, 637
BstPAI GACNNNNGTC 2 cut(s) 32, 663
BstSCI CCNGG 2 cut(s) 107, 450
BstSLI GKGCMC 2 cut(s) 1165, 1267
BstUI CGCG 3 cut(s) 81, 212, 228
BstV1I GCAGC 3 cut(s) 325, 355, 640
BstV2I GAAGAC 1 cut(s) 1123
BstZI CGGCCG 1 cut(s) 82
BsuI GTATCC 4 cut(s) 69, 882, 976, 1181
BsuRI GGCC 4 cut(s) 84, 446, 1008, 1075
BtgI CCRYGG 1 cut(s) 1273
BtsCI GGATG 4 cut(s) 307, 400, 952, 1332
BtsIMutI CAGTG 3 cut(s) 164, 735, 1032
BveI ACCTGC 2 cut(s) 286, 613
Cac8I GCNNGC 4 cut(s) 120, 283, 610, 1374
CfoI GCGC 2 cut(s) 91, 287
Cfr10I RCCGGY 2 cut(s) 950, 1368
Cfr13I GGNCC 2 cut(s) 61, 1006
CseI GACGC 2 cut(s) 151, 201
Csp6I GTAC 6 cut(s) 208, 322, 425, 860, 892, 1178
CviAII CATG 3 cut(s) 1087, 1175, 1274
CviQI GTAC 6 cut(s) 208, 322, 425, 860, 892, 1178
DdeI CTNAG 4 cut(s) 390, 699, 900, 1035
DpnI GATC 3 cut(s) 545, 966, 1362
DpnII GATC 3 cut(s) 543, 964, 1360
DriI GACNNNNNGTC 1 cut(s) 909
EaeI YGGCCR 2 cut(s) 82, 444
EagI CGGCCG 1 cut(s) 82
Eam1104I CTCTTC 1 cut(s) 1319
Eam1105I GACNNNNNGTC 1 cut(s) 909
EarI CTCTTC 1 cut(s) 1319
Ecl136II GAGCTC 1 cut(s) 41
EclXI CGGCCG 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 1273
Eco147I AGGCCT 1 cut(s) 1075
Eco24I GRGCYC 1 cut(s) 43
Eco31I GGTCTC 1 cut(s) 746
Eco47I GGWCC 1 cut(s) 61
Eco52I CGGCCG 1 cut(s) 82
Eco53kI GAGCTC 1 cut(s) 41
Eco57I CTGAAG 3 cut(s) 475, 1224, 1237
Eco72I CACGTG 1 cut(s) 1211
EcoICRI GAGCTC 1 cut(s) 41
EcoT14I CCWWGG 1 cut(s) 1273
EcoT38I GRGCYC 1 cut(s) 43
ErhI CCWWGG 1 cut(s) 1273
Esp3I CGTCTC 1 cut(s) 917
FaeI CATG 3 cut(s) 1090, 1178, 1277
FalI AAGNNNNNCTT 1 cut(s) 38
FaqI GGGAC 1 cut(s) 989
FatI CATG 3 cut(s) 1086, 1174, 1273
FauI CCCGC 1 cut(s) 171
Fnu4HI GCNGC 6 cut(s) 82, 226, 339, 369, 444, 629
FokI GGATG 4 cut(s) 314, 407, 959, 1319
FriOI GRGCYC 1 cut(s) 43
Fsp4HI GCNGC 6 cut(s) 82, 226, 339, 369, 444, 629
FspBI CTAG 1 cut(s) 1373
FspI TGCGCA 1 cut(s) 90
GlaI GCGC 2 cut(s) 90, 286
GluI GCNGC 6 cut(s) 82, 226, 339, 369, 444, 629
GsuI CTGGAG 1 cut(s) 1316
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 4 cut(s) 84, 446, 1008, 1075
HapII CCGG 4 cut(s) 108, 451, 951, 1369
HgaI GACGC 2 cut(s) 151, 201
HhaI GCGC 2 cut(s) 91, 287
Hin1II CATG 3 cut(s) 1090, 1178, 1277
Hin6I GCGC 2 cut(s) 89, 285
HinP1I GCGC 2 cut(s) 89, 285
HincII GTYRAC 1 cut(s) 796
HindII GTYRAC 1 cut(s) 796
HinfI GANTC 5 cut(s) 230, 465, 472, 904, 1247
HpaI GTTAAC 1 cut(s) 796
HpaII CCGG 4 cut(s) 108, 451, 951, 1369
HphI GGTGA 4 cut(s) 214, 615, 1043, 1154
Hpy166II GTNNAC 5 cut(s) 150, 735, 796, 1163, 1265
Hpy188III TCNNGA 6 cut(s) 166, 469, 743, 1106, 1358, 1364
Hpy8I GTNNAC 5 cut(s) 150, 735, 796, 1163, 1265
Hpy99I CGWCG 3 cut(s) 167, 217, 1100
HpyCH4III ACNGT 7 cut(s) 154, 172, 326, 885, 1115, 1231, 1262
HpyCH4IV ACGT 5 cut(s) 591, 910, 1168, 1198, 1210
HpyCH4V TGCA 4 cut(s) 281, 307, 1163, 1265
HpyF10VI GCNNNNNNNGC 3 cut(s) 502, 614, 637
HpyF3I CTNAG 4 cut(s) 390, 699, 900, 1035
HpySE526I ACGT 5 cut(s) 591, 910, 1168, 1198, 1210
Hsp92II CATG 3 cut(s) 1090, 1178, 1277
HspAI GCGC 2 cut(s) 89, 285
KspAI GTTAAC 1 cut(s) 796
Kzo9I GATC 3 cut(s) 543, 964, 1360
LguI GCTCTTC 1 cut(s) 1319
LmnI GCTCC 5 cut(s) 38, 636, 768, 1297, 1381
Lsp1109I GCAGC 3 cut(s) 325, 355, 640
LweI GCATC 3 cut(s) 730, 937, 1299
MaeI CTAG 1 cut(s) 1373
MaeII ACGT 5 cut(s) 591, 910, 1168, 1198, 1210
MaeIII GTNAC 2 cut(s) 166, 621
MalI GATC 3 cut(s) 545, 966, 1362
MboI GATC 3 cut(s) 543, 964, 1360
MboII GAAGA 5 cut(s) 14, 16, 481, 1123, 1306
MfeI CAATTG 1 cut(s) 276
MhlI GDGCHC 3 cut(s) 43, 1165, 1267
MluCI AATT 6 cut(s) 276, 357, 835, 1117, 1184, 1215
MluI ACGCGT 1 cut(s) 210
MlyI GAGTC 3 cut(s) 239, 481, 898
MmeI TCCRAC 2 cut(s) 16, 460
MroXI GAANNNNTTC 2 cut(s) 146, 930
MseI TTAA 3 cut(s) 579, 795, 887
MslI CAYNNNNRTG 1 cut(s) 726
MspA1I CMGCKG 2 cut(s) 178, 443
MspI CCGG 4 cut(s) 108, 451, 951, 1369
MspR9I CCNGG 2 cut(s) 109, 452
MunI CAATTG 1 cut(s) 276
Mva1269I GAATGC 1 cut(s) 370
MvnI CGCG 3 cut(s) 81, 212, 228
MwoI GCNNNNNNNGC 3 cut(s) 502, 614, 637
NciI CCSGG 2 cut(s) 109, 452
NcoI CCATGG 1 cut(s) 1273
NdeII GATC 3 cut(s) 543, 964, 1360
NheI GCTAGC 1 cut(s) 1372
NlaIII CATG 3 cut(s) 1090, 1178, 1277
NmuCI GTSAC 2 cut(s) 166, 621
NsbI TGCGCA 1 cut(s) 90
NspV TTCGAA 1 cut(s) 879
PaqCI CACCTGC 1 cut(s) 286
PceI AGGCCT 1 cut(s) 1075
PciSI GCTCTTC 1 cut(s) 1319
PctI GAATGC 1 cut(s) 370
PdmI GAANNNNTTC 2 cut(s) 146, 930
PfeI GAWTC 2 cut(s) 465, 1247
PkrI GCNGC 6 cut(s) 83, 227, 340, 370, 445, 630
PleI GAGTC 3 cut(s) 238, 480, 898
PmaCI CACGTG 1 cut(s) 1211
PmlI CACGTG 1 cut(s) 1211
PpsI GAGTC 3 cut(s) 238, 480, 898
Ppu21I YACGTR 2 cut(s) 592, 1211
PshAI GACNNNNGTC 2 cut(s) 32, 663
Psp124BI GAGCTC 1 cut(s) 43
PspCI CACGTG 1 cut(s) 1211
PspPI GGNCC 2 cut(s) 61, 1006
RsaI GTAC 6 cut(s) 209, 323, 426, 861, 893, 1179
RsaNI GTAC 6 cut(s) 208, 322, 425, 860, 892, 1178
RseI CAYNNNNRTG 1 cut(s) 726
SacI GAGCTC 1 cut(s) 43
SapI GCTCTTC 1 cut(s) 1319
SaqAI TTAA 3 cut(s) 579, 795, 887
SatI GCNGC 6 cut(s) 82, 226, 339, 369, 444, 629
Sau3AI GATC 3 cut(s) 543, 964, 1360
Sau96I GGNCC 2 cut(s) 61, 1006
ScaI AGTACT 2 cut(s) 426, 893
SchI GAGTC 3 cut(s) 239, 481, 898
ScrFI CCNGG 2 cut(s) 109, 452
SduI GDGCHC 3 cut(s) 43, 1165, 1267
SfaNI GCATC 3 cut(s) 730, 937, 1299
SfuI TTCGAA 1 cut(s) 879
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 1 cut(s) 726
Sse9I AATT 6 cut(s) 276, 357, 835, 1117, 1184, 1215
SseBI AGGCCT 1 cut(s) 1075
SsiI CCGC 8 cut(s) 81, 178, 226, 443, 626, 923, 1054, 1336
SspMI CTAG 1 cut(s) 1373
SstI GAGCTC 1 cut(s) 43
StuI AGGCCT 1 cut(s) 1075
StyD4I CCNGG 2 cut(s) 107, 450
StyI CCWWGG 1 cut(s) 1273
TaaI ACNGT 7 cut(s) 154, 172, 326, 885, 1115, 1231, 1262
TaiI ACGT 5 cut(s) 594, 913, 1171, 1201, 1213
TaqI TCGA 9 cut(s) 12, 215, 233, 373, 675, 744, 879, 1138, 1194
TasI AATT 6 cut(s) 276, 357, 835, 1117, 1184, 1215
TatI WGTACW 3 cut(s) 321, 424, 891
TauI GCSGC 3 cut(s) 84, 228, 446
TfiI GAWTC 2 cut(s) 465, 1247
Tru1I TTAA 3 cut(s) 579, 795, 887
Tru9I TTAA 3 cut(s) 579, 795, 887
TscAI CASTG 3 cut(s) 164, 742, 1039
TseFI GTSAC 2 cut(s) 166, 621
TseI GCWGC 3 cut(s) 338, 368, 628
Tsp45I GTSAC 2 cut(s) 166, 621
TspDTI ATGAA 2 cut(s) 17, 1123
TspGWI ACGGA 2 cut(s) 44, 1050
TspRI CASTG 3 cut(s) 164, 742, 1039
VneI GTGCAC 2 cut(s) 1161, 1263
VpaK11BI GGWCC 1 cut(s) 61
XcmI CCANNNNNNNNNTGG 2 cut(s) 476, 1280
XmnI GAANNNNTTC 2 cut(s) 146, 930
XspI CTAG 1 cut(s) 1373
ZrmI AGTACT 2 cut(s) 426, 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.