Rroxscaffold_7G00174480

Spermatogenesis-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012290
Physical Location & Seq
Reverse (-)
14134692 .. 14146143
11452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00174480.1

Sequence Viewer

Length: 4845 bp
ATGGCACCACCAACTTCCTCCATTCCGGTTCCTTCTAAACTTTCACCAACCTCTTCTTCTTCTTCCTCTCCCCACTCTTCTCTGCTTCTCCACAATTCTATGCTCAAAAGGCTTTCTCAGCGCTTCATCTCTTCACACCCAACAAAACTACTCTCCTCACCCAGAAAAACACACCCTCTTCCTCACTCCTTGATTTCTCATCGACCCTTTTACGCTTCCAAGGTTTTGGCCATGGCTCACCAACACACTTCGAACCCAACTTCCCACAAGCACACCAATCGCCTCGCTGCCGAGCACAGCCCCTATCTCCTTCAACACGCCCACAACCCGGTTGATTGGTATCCATGGAGTGAAGAGGCTTTCGCGGAAGCTCGAAAGAGAGATGTGCCCATCTTCTTATCAATTGGGTACAGCACATGCCATTGGTGTCACGTTATGGAGGTCGAGTCTTTTGAGGATGAAGGCATTGCCAAGTTGCTGAATGAGTGGTTTGTTAGTATCAAGGTGGATCGGGAGGAAAGACCAGATGTGGATAAGGTATACATGACCTATGTACAGGCTCTGTATGGTGGTGGAGGCTGGCCACTGACGGTGTTCCTTTCACCAGATTTGAAACCTTTGATGGGTGGAACTTACTTTCCCCCAGAGGACAAGTATGGAAGACCTGGATTTAAGACCATACTTGGAAAAGTGAAAGAAGCCTGGGAGACCAAGAGGGATGTGCTTGTCAAGAGTGGAGCCTTTGCAATTGAACAGTTGTCAGAAGCATTGTCAACAAGTGCAAGTTCTAAAAAATTGTCAGATGGGATTCCAAAGAATGCTTTGCGTCTATGTGCTGAGCAAGTAGTCAAAGATCTGCTGCACACCTTCCTTCCTGTTCTTTCAATTTTGAATAAGGGCATTTTAGGAAATATGAAAGAGGATGTGCACAGGTCACCAATCTCTCATAAGCTTCTAAGAAGAGGAGCTTGTAATGAAATTCTGTGCTTGATTGAGTGGTTTATCTTTATTTTCCCTGAGCCTCCTGCTTTACTTTTCTTACAGCTTTCTCAAAGTTATGATTCAAAGTTTGGTGGGTTTGGATCTGCCCCAAAGTTTCCTAGACCAGTTGAGATTCAGCTGATGCTTTATTACTCAAAGAAGTCGGAGGAAACTGGAAATTCTGGCAAAGCCAATGAAGTTCTGAAAAAGGTTCTGTTTACCTTGCAATGCATGGCAAAAGGGGGAATTCACGATCACGTCGGAGGTGGCTTCCACCGATATAGCGTGGATGAGTGCTGGCATGTACCGCACTTTGAGAAGATGTTGTATGATCAGGGCCAACTTGCTAATGTCTACCTAGATGCCTTTTCTATTACCAAAGATGTATTCTATGCATCTATTGCTCGGGATGTTCTTGATTATCTGAGGAGAGACATGATTGGACCAGATGGCGAAATATATTCAGCAGAAGATGCTGATAGTGCTGAATCTGAAGGTGCATCCAGAAAAAAGGAAGGAGCATTCTATGTTTGGTCTAGCAAAGAGGTTGAAGACATACTTGGAGAAGATGCAACTCTTTTTAAGGACCACTACTATATAAAGCCTTCCGGTAACTGTGACCTCTCCAGAATGAGTGATCCTCATAATGAATTCAAGAGTAAGAATGTGATGATTGAGAGAGAAAGTCATTCCGAGGCATCAAATTTTTCCATGCCGGTTGAGAAATACCTCGATATTCTGGGTCAGAGCAGGCGAAAGCTTTTTGAAATAAGATGTAGTCGACCAAGACCACATTTGGATGATAAGGTAATTGTAGCATGGAATGGACTGGCCATCTCAGCCTTTGCAAGAGCCTCTAAAATTCTAAAGAATGAACCTAAGGAGATCAAGTTCAACTTTCCTGTTGTTGGTTGTGATGATGAAATATTTCTTGATAAAGAGGGAGGCGGCTATTTTAATACCCCAGGTGATGACCCTAGTGTTCTCCTTCGTGTGAAGGAAGATCATGATGGGGCAGAGCCCTCTGGAAACTCAGTTTCTGTGATCAACCTTGTGAGATTGGCATCAATACTATCTGGCAATAGGTCTGAGCATTACAGACAGGAAGCTGAACATGTTCTGGCGGTTTTTGAGACAAGATTAAACGACATGGCCATGGCAGTACCCTTAATGTGTTGTGCTGCAGACATGCTCTCTGTTCCTTCTCGGAAGCAAGTTGTCTTGGTTGGCCAGAAAACTAGAGAGTTTGAAAACATGCTTGCCGCAGCTCATGCATCATATGATACCAACAAAACGGTTATTCACATAGATCCAACTAATTCGGAAGAGTTGCAATTCTGGGAAGACAAAAACAGCAACATTGCGCTCATGGCAAAGAATCATGTTGTTGCAGACAAAGTGTTTGCTCTCGTCTGCCAAAACTTTACTTGCAGTGCGCCTGTTGCTGACCCTGGATCCCTGGAGGCTCTGCTCTCCAAGAAATCAGAAATTCTCATCGAATTATCTTCGGACAAATGTATGCAAAGGAAACATGACAACGCGATTAACTCATGTGGGCTTTCACCAGAAGCCGCCATCGTGGCATCCCACAAGATGCCCATATCTCAAAGGTTGTGCGGATACACCCACATGTTCTGGAGGCAAATGCCCAGGAAACCTCGAGTTTTTGTGCTCCATAGGAATGTCAAGGCTCGACCTTGCAAAAACACTGAGCTACAACCCAATTCTTTTTACGCGAAGCTTGAAAAACTGCATTATACCCTGTTATACTTTCCTCCAAAGCGTGTGAACTTGTGGGTGAAGTCGAACAATTGGGATTTGACCCTCAAAACTCGAGCTTTATGCATGCCATGCACGCATTATATGGGAAGAAGACGACATGGAAGCGAAAATGAAGAGGCTTATAGGAGGTGGGGTTGGTCGAATAATAATATTCTTTCTGCCTTCAGCTTGGAGAAGAGAATCATCCCAAGGTGTTCAATTGTTAGAGTTTTGTTGTTGAAAGGATTGATAGAAGAGGGGGAGAACTTGAGTTTGGCTACTGTGATTTCGAGGTCGGAGGAGTCCTTCTTGGATAGAATAGGCAATAATGAGATCACGGTTTTAGTAAATGATGCGGAGAAGGGTAGTGACATTAATCCACAAGAAGCTCAACAAACTCTTGAAATTGCAGAAGCTAACTTGAGGAAAGCTGAAGGCAAGAGACAAACAATTGAGGCAAATTTAGCTCTCAGACGAGCTAGGACACGAGTCGAGGCTATCAATGTGATGTCGTCGATCTTTGGAAGCGCAACTCATGGATTTTCAGGGGAAATGATTGATGAAAGAGGAAGGGTTGAATATGAGTACTATTACTACTACTTCAGAGAAATACTTCTTGACAAAGGATTGTCTAGGGTGATAGTGTTGAATCCGGAGGTGCTACTAGAGAAGGCGCTGGAAAACAAGGTGGTCCCGAAGCCGCCGGTGGAAAACAACAACGTGGTGGGAGTTCCGAAGAATGGGGTGGAACTAGGAGTGAAGAAGGACGGGGTCGTCGACGGAGTGCCGAAGGCTGCAGCCTGGAGTAGTGCATTAAGCAATCGGTTTCCACTTTGCGAAGTTTTGTCACATTGTAACTGGGCAGCAAATGGGAAAGAAGGTCGTCATGGGCCACCTCCAATGGAGGAGCAGAGCCCAAATTTTGGCCCAAAATTTCAATTAAATAGAAGGGTTTGGAAATTGGGGGATTGGCCGCTTCCTTGTTCTCAAAACAGTAGAGTATGGCACCCCACTCAATTTTCAGCAAGAGCCTCAGATTTTGTCGTGTGTTTTACAGAAATGGGCACCCAGTCGGGGGGAATGGCTTCGCATTTTGGTCTTCAAAAGCAGTTGTTTTGTACAAGTCTCACCGGCAGGTCCGAAATCCCAGGCGGCCAAGATGATTCTACAGTCTCCTACCTGGTTAACTCGTGTGGGTTGTCCCCAGAATCCGCTACCCTAGTATCCCACGAGGTGAAGCTTCATTCTTTAGAGAAACCAGACTCCGTTTTAGCGCTTTTGAAACGCTATGAATTCTCGGAGGCCCAGATTTCAAAGGTTGTGCTGAGACTCCCACATATTCTTTTGGCCGATGTGGAGCAAACCCTTTTGCCAAACCTCGAGTTTTACTGCTCCATAGGAATGTCAAGGCTCCACCTTGCAAGAACACTGAGCTACAACCCATGGCTCATGAGTCTAAGCTTGAAAAACCATATTGTACCAACGTATAACTTCTTCAAGAGCGTGGTGCTCTCTGATGTCAAAGTGATCCATATTTTGAAGCACAAGTCCTGGATTTTCAGGGAAAATTTATCCAAGAATTTGATACCCAATATTGAGCTTGCGAGAGAATTGGGCATTCCCCAGTCCTGTATTGCCCTCTTGCTAACTAGTTATACTGATGTAGTGATGAAAAAGCCTGAATTGTTCAGTCAACTTGTGCATCAAGTCACAGAAATGGGATTTGACCCTCAAAATTTGAGCTTTGTGCAGGCCATACACGCATTATATGGGAAAGAGACTACATGGAAACGATGTCAAGAGGTTTATAGGAGGTGGGGTTGGTCTGAGAATCATATACGTTCTGCTTTCAGGGTGTCTCCACTGTGTATGGTTATGTCAGAGAAGAAACTGATGGGAACAATGGATTTCTTAGTGAACAAGATGGGGTGGCAGTCACAAACAATTGCGAAATATCCCCATGTATTGAGTTACAGCTTGCAGAAGAGATTCATCCCAAGGTTTTCAGTTGTTCGAGTTTTGATTTTGAAAGGATTGATAGAAGAGGAGAACTTGAGTTTGGCTAATGGGATTTCGAGGTCAGAGAAGTACTTCTTTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
Thioredox_DsbH

Protein Analysis

1614

Amino Acids

182.31

Weight (kDa)

8.5

Isoelectric Point (pI)

53.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredox_DsbH PF03190 92 - 252 2e-75 Protein of unknown function, DUF255
Thioredoxin_7 PF13899 112 - 202 4.2e-09 Thioredoxin-like
ATP-synt_DE PF00401 1038 - 1081 1.4e-06 ATP synthase, Delta/Epsilon chain, long alpha-helix domain
mTERF PF02536 1338 - 1494 7.9e-21 mTERF
mTERF PF02536 1507 - 1612 1.4e-13 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012290)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03200 AT4G03200
fragaria_vesca FvH4_2g25030
malus_domestica MD03G1280600.v1.1
prunus_persica Prupe.8G255100_v2.0.a1
pyrus_communis pycom03g22230
rosa_chinensis RchiOBHm_Chr6g0293001
rosa_laevigata RLG00000011975
rosa_multiflora Rmu_sc0001788.1_g000005 Rmu_sc0003615.1_g000002 Rmu_sc0028741.1_g000001
rosa_roxburghii Rroxscaffold_7G00174480
rosa_rugosa Rorug06G0231900 Rorug06G0232000
rosa_samantha Rh6AG344700 Rh6BG352100 Rh6CG359300 Rh6DG344800
rosa_wichuraiana Rw6G030080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3515
Acc36I ACCTGC 1 cut(s) 3867
AccB1I GGYRCC 3 cut(s) 4, 3747, 3806
AccB7I CCANNNNNTGG 1 cut(s) 3665
AccI GTMKAC 4 cut(s) 540, 1335, 1762, 3519
AccII CGCG 3 cut(s) 365, 2522, 2717
AccIII TCCGGA 1 cut(s) 3394
AclWI GGATC 7 cut(s) 516, 1090, 1613, 2285, 2430, 2443, 4264
AcoI YGGCCR 8 cut(s) 228, 581, 1812, 2133, 2209, 3713, 3895, 4089
AcuI CTGAAG 4 cut(s) 1494, 2911, 3195, 3328
AdeI CACNNNGTG 1 cut(s) 3976
AfaI GTAC 8 cut(s) 410, 555, 1287, 2145, 3329, 3862, 4221, 4832
AfeI AGCGCT 2 cut(s) 122, 4017
AfiI CCNNNNNNNGG 7 cut(s) 328, 623, 1889, 3482, 3665, 3816, 3817
AflIII ACRYGT 2 cut(s) 2095, 2610
AhlI ACTAGT 1 cut(s) 4391
AjiI CACGTC 1 cut(s) 1240
AloI GAACNNNNNNTCC 6 cut(s) 245, 277, 622, 654, 1856, 1888
Alw21I GWGCWC 4 cut(s) 297, 930, 2655, 4254
Alw26I GTCTC 9 cut(s) 701, 1407, 2108, 3178, 3872, 3919, 4063, 4514, 4605
Alw44I GTGCAC 1 cut(s) 926
AlwI GGATC 7 cut(s) 516, 1090, 1613, 2285, 2430, 2443, 4264
AlwNI CAGNNNCTG 2 cut(s) 562, 2021
Ama87I CYCGRG 4 cut(s) 1386, 2640, 2814, 4121
Aor13HI TCCGGA 1 cut(s) 3394
Aor51HI AGCGCT 2 cut(s) 122, 4017
ApaLI GTGCAC 1 cut(s) 926
ApeKI GCWGC 7 cut(s) 287, 859, 2162, 2246, 3536, 3539, 3605
ArsI GACNNNNNNTTYG 2 cut(s) 2366, 2398
AseI ATTAAT 1 cut(s) 3117
Asp700I GAANNNNTTC 6 cut(s) 1908, 2097, 3354, 3826, 4730, 4832
AspLEI GCGC 6 cut(s) 123, 2347, 2419, 3272, 3418, 4018
AspS9I GGNCC 8 cut(s) 1318, 1424, 1567, 3433, 3632, 3668, 3879, 4045
AsuC2I CCSGG 1 cut(s) 329
AsuII TTCGAA 1 cut(s) 251
AvaI CYCGRG 4 cut(s) 1386, 2640, 2814, 4121
AvaII GGWCC 4 cut(s) 1424, 1567, 3433, 3879
AxyI CCTNAGG 1 cut(s) 1860
BaeGI GKGCMC 3 cut(s) 390, 930, 3809
BaeI ACNNNNGTAYC 2 cut(s) 3948, 3981
BalI TGGCCA 5 cut(s) 230, 583, 1814, 2135, 2211
BamHI GGATCC 1 cut(s) 2435
BanI GGYRCC 3 cut(s) 4, 3747, 3806
BanII GRGCYC 2 cut(s) 2004, 3659
BarI GAAGNNNNNNTAC 2 cut(s) 4220, 4252
BauI CACGAG 3 cut(s) 3228, 3931, 3971
BbsI GAAGAC 5 cut(s) 667, 1539, 2331, 2860, 3833
Bbv12I GWGCWC 4 cut(s) 297, 930, 2655, 4254
BbvI GCAGC 7 cut(s) 274, 846, 2149, 2258, 3523, 3551, 3617
BccI CCATC 9 cut(s) 398, 616, 797, 1424, 1823, 1985, 2564, 4630, 4660
BcgI CGANNNNNNTGC 4 cut(s) 260, 294, 2805, 2839
BciVI GTATCC 3 cut(s) 351, 2594, 3976
BclI TGATCA 2 cut(s) 1312, 2025
BcnI CCSGG 1 cut(s) 329
BcoDI GTCTC 9 cut(s) 701, 1407, 2108, 3178, 3872, 3919, 4063, 4514, 4605
BcuI ACTAGT 1 cut(s) 4391
BfmI CTRYAG 3 cut(s) 2163, 3537, 3909
BfoI RGCGCY 3 cut(s) 124, 3419, 4019
BfuAI ACCTGC 1 cut(s) 3867
BfuI GTATCC 3 cut(s) 351, 2594, 3976
BglI GCCNNNNNGGC 1 cut(s) 2561
BglII AGATCT 1 cut(s) 853
BlpI GCTNAGC 1 cut(s) 837
BmcAI AGTACT 2 cut(s) 3329, 4832
Bme18I GGWCC 4 cut(s) 1424, 1567, 3433, 3879
BmeT110I CYCGRG 4 cut(s) 1386, 2640, 2814, 4121
BmgBI CACGTC 1 cut(s) 1240
BmgT120I GGNCC 8 cut(s) 1318, 1424, 1567, 3433, 3632, 3668, 3879, 4045
BmiI GGNNCC 8 cut(s) 6, 30, 739, 2437, 3435, 3749, 3808, 4154
BmrI ACTGGG 3 cut(s) 3610, 3805, 4360
BmuI ACTGGG 3 cut(s) 3610, 3805, 4360
BoxI GACNNNNGTC 1 cut(s) 3230
BpiI GAAGAC 5 cut(s) 667, 1539, 2331, 2860, 3833
BplI GAGNNNNNCTC 2 cut(s) 1606, 1638
BpmI CTGGAG 4 cut(s) 1591, 2462, 2638, 3565
Bpu10I CCTNAGC 1 cut(s) 1017
Bpu1102I GCTNAGC 1 cut(s) 837
Bpu14I TTCGAA 1 cut(s) 251
BpuEI CTTGAG 3 cut(s) 3031, 3184, 4816
BpuMI CCSGG 1 cut(s) 329
BsaBI GATNNNNATC 2 cut(s) 339, 2044
BsaI GGTCTC 1 cut(s) 701
BsaWI WCCGGW 3 cut(s) 25, 1589, 3394
BsaXI ACNNNNNCTCC 8 cut(s) 431, 461, 1856, 1886, 2881, 2911, 4546, 4576
Bsc4I CCNNNNNNNGG 7 cut(s) 328, 623, 1889, 3482, 3665, 3816, 3817
Bse118I RCCGGY 3 cut(s) 1696, 3445, 3872
Bse1I ACTGG 6 cut(s) 1106, 1159, 1815, 3605, 3811, 4366
Bse21I CCTNAGG 1 cut(s) 1860
Bse3DI GCAATG 3 cut(s) 465, 1214, 2340
Bse8I GATNNNNATC 2 cut(s) 339, 2044
BseAI TCCGGA 1 cut(s) 3394
BseJI GATNNNNATC 2 cut(s) 339, 2044
BseLI CCNNNNNNNGG 7 cut(s) 328, 623, 1889, 3482, 3665, 3816, 3817
BseMI GCAATG 3 cut(s) 465, 1214, 2340
BseNI ACTGG 6 cut(s) 1106, 1159, 1815, 3605, 3811, 4366
BseRI GAGGAG 6 cut(s) 145, 978, 1423, 3056, 3662, 4802
BseSI GKGCMC 3 cut(s) 390, 930, 3809
BseXI GCAGC 7 cut(s) 274, 846, 2149, 2258, 3523, 3551, 3617
BsgI GTGCAG 2 cut(s) 845, 4511
Bsh1236I CGCG 3 cut(s) 365, 2522, 2717
BshNI GGYRCC 3 cut(s) 4, 3747, 3806
BsiHKAI GWGCWC 4 cut(s) 297, 930, 2655, 4254
BsiHKCI CYCGRG 4 cut(s) 1386, 2640, 2814, 4121
BsiSI CCGG 7 cut(s) 26, 329, 1590, 1697, 3395, 3446, 3873
BslFI GGGAC 2 cut(s) 3419, 3928
BslI CCNNNNNNNGG 7 cut(s) 328, 623, 1889, 3482, 3665, 3816, 3817
BsmAI GTCTC 9 cut(s) 701, 1407, 2108, 3178, 3872, 3919, 4063, 4514, 4605
BsmFI GGGAC 2 cut(s) 3419, 3928
BsmI GAATGC 3 cut(s) 823, 1502, 4359
Bso31I GGTCTC 1 cut(s) 701
BsoBI CYCGRG 4 cut(s) 1386, 2640, 2814, 4121
Bsp119I TTCGAA 1 cut(s) 251
Bsp1286I GDGCHC 8 cut(s) 297, 390, 930, 2004, 2655, 3659, 3809, 4254
Bsp13I TCCGGA 1 cut(s) 3394
Bsp1407I TGTACA 2 cut(s) 553, 3860
Bsp1720I GCTNAGC 1 cut(s) 837
Bsp19I CCATGG 4 cut(s) 231, 344, 2136, 4184
BspEI TCCGGA 1 cut(s) 3394
BspFNI CGCG 3 cut(s) 365, 2522, 2717
BspHI TCATGA 2 cut(s) 1987, 4191
BspLI GGNNCC 8 cut(s) 6, 30, 739, 2437, 3435, 3749, 3808, 4154
BspMAI CTGCAG 2 cut(s) 2167, 3541
BspMI ACCTGC 1 cut(s) 3867
BspPI GGATC 7 cut(s) 516, 1090, 1613, 2285, 2430, 2443, 4264
BspT104I TTCGAA 1 cut(s) 251
BspT107I GGYRCC 3 cut(s) 4, 3747, 3806
BspTNI GGTCTC 1 cut(s) 701
BsrDI GCAATG 3 cut(s) 465, 1214, 2340
BsrFI RCCGGY 3 cut(s) 1696, 3445, 3872
BsrGI TGTACA 2 cut(s) 553, 3860
BsrI ACTGG 6 cut(s) 1106, 1159, 1815, 3605, 3811, 4366
BssAI RCCGGY 3 cut(s) 1696, 3445, 3872
BssNAI GTATAC 1 cut(s) 541
BssSI CACGAG 3 cut(s) 3228, 3931, 3971
BssT1I CCWWGG 7 cut(s) 219, 231, 344, 2136, 2951, 4184, 4739
Bst1107I GTATAC 1 cut(s) 541
Bst2BI CACGAG 3 cut(s) 3228, 3931, 3971
Bst4CI ACNGT 9 cut(s) 592, 756, 1598, 2278, 3025, 3082, 3737, 3913, 4608
BstAPI GCANNNNNTGC 4 cut(s) 1382, 1454, 2252, 2832
BstAUI TGTACA 2 cut(s) 553, 3860
BstBI TTCGAA 1 cut(s) 251
BstC8I GCNNGC 9 cut(s) 581, 1280, 1733, 2241, 2828, 2837, 4344, 4494, 4721
BstDSI CCRYGG 4 cut(s) 231, 344, 2136, 4184
BstEII GGTNACC 1 cut(s) 933
BstFNI CGCG 3 cut(s) 365, 2522, 2717
BstH2I RGCGCY 3 cut(s) 124, 3419, 4019
BstHHI GCGC 6 cut(s) 123, 2347, 2419, 3272, 3418, 4018
BstMAI GTCTC 9 cut(s) 701, 1407, 2108, 3178, 3872, 3919, 4063, 4514, 4605
BstNSI RCATGY 7 cut(s) 420, 1286, 2099, 2173, 2239, 2614, 2830
BstPAI GACNNNNGTC 1 cut(s) 3230
BstPI GGTNACC 1 cut(s) 933
BstSFI CTRYAG 3 cut(s) 2163, 3537, 3909
BstSLI GKGCMC 3 cut(s) 390, 930, 3809
BstUI CGCG 3 cut(s) 365, 2522, 2717
BstV1I GCAGC 7 cut(s) 274, 846, 2149, 2258, 3523, 3551, 3617
BstV2I GAAGAC 5 cut(s) 667, 1539, 2331, 2860, 3833
BstX2I RGATCY 4 cut(s) 853, 1082, 2290, 2435
BstXI CCANNNNNNTGG 1 cut(s) 226
BstYI RGATCY 4 cut(s) 853, 1082, 2290, 2435
BstZ17I GTATAC 1 cut(s) 541
Bsu36I CCTNAGG 1 cut(s) 1860
BsuI GTATCC 3 cut(s) 351, 2594, 3976
BtgI CCRYGG 4 cut(s) 231, 344, 2136, 4184
BtrI CACGTC 1 cut(s) 1240
BtsI GCAGTG 1 cut(s) 2419
BtsIMutI CAGTG 5 cut(s) 584, 2419, 2688, 4169, 4604
BveI ACCTGC 1 cut(s) 3867
Cac8I GCNNGC 9 cut(s) 581, 1280, 1733, 2241, 2828, 2837, 4344, 4494, 4721
CaiI CAGNNNCTG 2 cut(s) 562, 2021
CciI TCATGA 2 cut(s) 1987, 4191
CfoI GCGC 6 cut(s) 123, 2347, 2419, 3272, 3418, 4018
Cfr10I RCCGGY 3 cut(s) 1696, 3445, 3872
Cfr13I GGNCC 8 cut(s) 1318, 1424, 1567, 3433, 3632, 3668, 3879, 4045
CseI GACGC 1 cut(s) 815
CsiI ACCWGGT 1 cut(s) 3921
Csp6I GTAC 8 cut(s) 409, 554, 1286, 2144, 3328, 3861, 4220, 4831
CviQI GTAC 8 cut(s) 409, 554, 1286, 2144, 3328, 3861, 4220, 4831
DraIII CACNNNGTG 1 cut(s) 3976
DrdI GACNNNNNNGTC 1 cut(s) 3515
DseDI GACNNNNNNGTC 1 cut(s) 3515
EaeI YGGCCR 8 cut(s) 228, 581, 1812, 2133, 2209, 3713, 3895, 4089
Eco130I CCWWGG 7 cut(s) 219, 231, 344, 2136, 2951, 4184, 4739
Eco24I GRGCYC 2 cut(s) 2004, 3659
Eco31I GGTCTC 1 cut(s) 701
Eco47I GGWCC 4 cut(s) 1424, 1567, 3433, 3879
Eco47III AGCGCT 2 cut(s) 122, 4017
Eco57I CTGAAG 4 cut(s) 1494, 2911, 3195, 3328
Eco81I CCTNAGG 1 cut(s) 1860
Eco88I CYCGRG 4 cut(s) 1386, 2640, 2814, 4121
Eco91I GGTNACC 1 cut(s) 933
EcoO65I GGTNACC 1 cut(s) 933
EcoRI GAATTC 3 cut(s) 1227, 1631, 4034
EcoT14I CCWWGG 7 cut(s) 219, 231, 344, 2136, 2951, 4184, 4739
EcoT22I ATGCAT 4 cut(s) 1214, 1378, 2257, 2828
EcoT38I GRGCYC 2 cut(s) 2004, 3659
ErhI CCWWGG 7 cut(s) 219, 231, 344, 2136, 2951, 4184, 4739
FalI AAGNNNNNCTT 7 cut(s) 952, 984, 1524, 1556, 1862, 1894, 4820
FaqI GGGAC 2 cut(s) 3419, 3928
FauNDI CATATG 1 cut(s) 2260
FbaI TGATCA 2 cut(s) 1312, 2025
FblI GTMKAC 4 cut(s) 540, 1335, 1762, 3519
FriOI GRGCYC 2 cut(s) 2004, 3659
GlaI GCGC 6 cut(s) 122, 2346, 2418, 3271, 3417, 4017
GsuI CTGGAG 4 cut(s) 1591, 2462, 2638, 3565
HaeII RGCGCY 3 cut(s) 124, 3419, 4019
HapII CCGG 7 cut(s) 26, 329, 1590, 1697, 3395, 3446, 3873
HgaI GACGC 1 cut(s) 815
HhaI GCGC 6 cut(s) 123, 2347, 2419, 3272, 3418, 4018
Hin6I GCGC 6 cut(s) 121, 2345, 2417, 3270, 3416, 4016
HinP1I GCGC 6 cut(s) 121, 2345, 2417, 3270, 3416, 4016
HincII GTYRAC 5 cut(s) 774, 1763, 3520, 3928, 4436
HindII GTYRAC 5 cut(s) 774, 1763, 3520, 3928, 4436
HindIII AAGCTT 5 cut(s) 950, 1739, 2720, 3980, 4201
HpaI GTTAAC 1 cut(s) 3928
HpaII CCGG 7 cut(s) 26, 329, 1590, 1697, 3395, 3446, 3873
Hpy99I CGWCG 4 cut(s) 1244, 3259, 3521, 3524
HpyCH4III ACNGT 9 cut(s) 592, 756, 1598, 2278, 3025, 3082, 3737, 3913, 4608
HpyCH4IV ACGT 5 cut(s) 432, 1239, 3462, 4226, 4582
HpySE526I ACGT 5 cut(s) 432, 1239, 3462, 4226, 4582
HspAI GCGC 6 cut(s) 121, 2345, 2417, 3270, 3416, 4016
Kpn2I TCCGGA 1 cut(s) 3394
Ksp22I TGATCA 2 cut(s) 1312, 2025
KspAI GTTAAC 1 cut(s) 3928
LmnI GCTCC 8 cut(s) 737, 965, 1499, 2658, 3649, 4099, 4139, 4158
Lsp1109I GCAGC 7 cut(s) 274, 846, 2149, 2258, 3523, 3551, 3617
MabI ACCWGGT 1 cut(s) 3921
MaeII ACGT 5 cut(s) 432, 1239, 3462, 4226, 4582
MfeI CAATTG 6 cut(s) 402, 747, 2791, 2961, 3192, 4686
MflI RGATCY 4 cut(s) 853, 1082, 2290, 2435
MhlI GDGCHC 8 cut(s) 297, 390, 930, 2004, 2655, 3659, 3809, 4254
MlsI TGGCCA 5 cut(s) 230, 583, 1814, 2135, 2211
MluNI TGGCCA 5 cut(s) 230, 583, 1814, 2135, 2211
MlyI GAGTC 6 cut(s) 455, 3053, 3240, 3998, 4065, 4204
MmeI TCCRAC 4 cut(s) 1125, 1222, 2318, 3018
Mox20I TGGCCA 5 cut(s) 230, 583, 1814, 2135, 2211
Mph1103I ATGCAT 4 cut(s) 1214, 1378, 2257, 2828
MroI TCCGGA 1 cut(s) 3394
MroXI GAANNNNTTC 6 cut(s) 1908, 2097, 3354, 3826, 4730, 4832
MscI TGGCCA 5 cut(s) 230, 583, 1814, 2135, 2211
MslI CAYNNNNRTG 6 cut(s) 1779, 2135, 2609, 2661, 4142, 4457
Msp20I TGGCCA 5 cut(s) 230, 583, 1814, 2135, 2211
MspA1I CMGCKG 1 cut(s) 1120
MspI CCGG 7 cut(s) 26, 329, 1590, 1697, 3395, 3446, 3873
MunI CAATTG 6 cut(s) 402, 747, 2791, 2961, 3192, 4686
Mva1269I GAATGC 3 cut(s) 823, 1502, 4359
MvnI CGCG 3 cut(s) 365, 2522, 2717
NciI CCSGG 1 cut(s) 329
NcoI CCATGG 4 cut(s) 231, 344, 2136, 4184
NdeI CATATG 1 cut(s) 2260
NlaIV GGNNCC 8 cut(s) 6, 30, 739, 2437, 3435, 3749, 3808, 4154
NmeAIII GCCGAG 1 cut(s) 316
NmuCI GTSAC 7 cut(s) 428, 933, 1598, 3110, 3588, 4450, 4677
NsiI ATGCAT 4 cut(s) 1214, 1378, 2257, 2828
NspI RCATGY 7 cut(s) 420, 1286, 2099, 2173, 2239, 2614, 2830
NspV TTCGAA 1 cut(s) 251
PaeI GCATGC 1 cut(s) 2830
PaeR7I CTCGAG 3 cut(s) 2640, 2814, 4121
PagI TCATGA 2 cut(s) 1987, 4191
PciI ACATGT 2 cut(s) 2095, 2610
PcsI WCGNNNNNNNCGW 1 cut(s) 3516
PctI GAATGC 3 cut(s) 823, 1502, 4359
PdmI GAANNNNTTC 6 cut(s) 1908, 2097, 3354, 3826, 4730, 4832
PflFI GACNNNGTC 1 cut(s) 3512
PflMI CCANNNNNTGG 1 cut(s) 3665
PfoI TCCNGGA 1 cut(s) 4292
PleI GAGTC 6 cut(s) 454, 3052, 3239, 3998, 4065, 4203
PpsI GAGTC 6 cut(s) 454, 3052, 3239, 3998, 4065, 4203
PscI ACATGT 2 cut(s) 2095, 2610
PshAI GACNNNNGTC 1 cut(s) 3230
PshBI ATTAAT 1 cut(s) 3117
PspEI GGTNACC 1 cut(s) 933
PspN4I GGNNCC 8 cut(s) 6, 30, 739, 2437, 3435, 3749, 3808, 4154
PspPI GGNCC 8 cut(s) 1318, 1424, 1567, 3433, 3632, 3668, 3879, 4045
PspXI VCTCGAGB 3 cut(s) 2640, 2814, 4121
PstI CTGCAG 2 cut(s) 2167, 3541
PstNI CAGNNNCTG 2 cut(s) 562, 2021
PsuI RGATCY 4 cut(s) 853, 1082, 2290, 2435
PsyI GACNNNGTC 1 cut(s) 3512
PvuII CAGCTG 1 cut(s) 1120
RsaI GTAC 8 cut(s) 410, 555, 1287, 2145, 3329, 3862, 4221, 4832
RsaNI GTAC 8 cut(s) 409, 554, 1286, 2144, 3328, 3861, 4220, 4831
RseI CAYNNNNRTG 6 cut(s) 1779, 2135, 2609, 2661, 4142, 4457
SalI GTCGAC 2 cut(s) 1761, 3518
Sau96I GGNCC 8 cut(s) 1318, 1424, 1567, 3433, 3632, 3668, 3879, 4045
ScaI AGTACT 2 cut(s) 3329, 4832
SchI GAGTC 6 cut(s) 455, 3053, 3240, 3998, 4065, 4204
SduI GDGCHC 8 cut(s) 297, 390, 930, 2004, 2655, 3659, 3809, 4254
SexAI ACCWGGT 1 cut(s) 3921
SfcI CTRYAG 3 cut(s) 2163, 3537, 3909
Sfr274I CTCGAG 3 cut(s) 2640, 2814, 4121
SfuI TTCGAA 1 cut(s) 251
SgrAI CRCCGGYG 1 cut(s) 3445
SgrDI CGTCGACG 1 cut(s) 3518
SinI GGWCC 4 cut(s) 1424, 1567, 3433, 3879
SlaI CTCGAG 3 cut(s) 2640, 2814, 4121
SmiMI CAYNNNNRTG 6 cut(s) 1779, 2135, 2609, 2661, 4142, 4457
SmlI CTYRAG 6 cut(s) 2640, 2814, 3010, 3163, 4121, 4795
SmoI CTYRAG 6 cut(s) 2640, 2814, 3010, 3163, 4121, 4795
SpeI ACTAGT 1 cut(s) 4391
SphI GCATGC 1 cut(s) 2830
SspI AATATT 3 cut(s) 1908, 2914, 4336
StyI CCWWGG 7 cut(s) 219, 231, 344, 2136, 2951, 4184, 4739
TaaI ACNGT 9 cut(s) 592, 756, 1598, 2278, 3025, 3082, 3737, 3913, 4608
TaiI ACGT 5 cut(s) 435, 1242, 3465, 4229, 4585
TatI WGTACW 4 cut(s) 553, 3327, 3860, 4830
TauI GCSGC 6 cut(s) 1932, 2246, 2555, 3445, 3718, 3897
TscAI CASTG 5 cut(s) 591, 2419, 2695, 4176, 4611
TseFI GTSAC 7 cut(s) 428, 933, 1598, 3110, 3588, 4450, 4677
TseI GCWGC 7 cut(s) 287, 859, 2162, 2246, 3536, 3539, 3605
Tsp45I GTSAC 7 cut(s) 428, 933, 1598, 3110, 3588, 4450, 4677
TspGWI ACGGA 2 cut(s) 3537, 3997
TspRI CASTG 5 cut(s) 591, 2419, 2695, 4176, 4611
Tth111I GACNNNGTC 1 cut(s) 3512
Van91I CCANNNNNTGG 1 cut(s) 3665
VneI GTGCAC 1 cut(s) 926
VpaK11BI GGWCC 4 cut(s) 1424, 1567, 3433, 3879
VspI ATTAAT 1 cut(s) 3117
XceI RCATGY 7 cut(s) 420, 1286, 2099, 2173, 2239, 2614, 2830
XhoI CTCGAG 3 cut(s) 2640, 2814, 4121
XmiI GTMKAC 4 cut(s) 540, 1335, 1762, 3519
XmnI GAANNNNTTC 6 cut(s) 1908, 2097, 3354, 3826, 4730, 4832
ZrmI AGTACT 2 cut(s) 3329, 4832
Zsp2I ATGCAT 4 cut(s) 1214, 1378, 2257, 2828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.