Spermatogenesis-associated protein
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G03200 AT4G03200 |
| fragaria_vesca | FvH4_2g25030 |
| malus_domestica | MD03G1280600.v1.1 |
| prunus_persica | Prupe.8G255100_v2.0.a1 |
| pyrus_communis | pycom03g22230 |
| rosa_chinensis | RchiOBHm_Chr6g0293001 |
| rosa_laevigata | RLG00000011975 |
| rosa_multiflora | Rmu_sc0001788.1_g000005 Rmu_sc0003615.1_g000002 Rmu_sc0028741.1_g000001 |
| rosa_roxburghii | Rroxscaffold_7G00174480 |
| rosa_rugosa | Rorug06G0231900 Rorug06G0232000 |
| rosa_samantha | Rh6AG344700 Rh6BG352100 Rh6CG359300 Rh6DG344800 |
| rosa_wichuraiana | Rw6G030080 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 2402 |
| Acc36I | ACCTGC | 1 cut(s) | 770 |
| AccB1I | GGYRCC | 1 cut(s) | 4 |
| AccI | GTMKAC | 3 cut(s) | 537, 1131, 1558 |
| AccII | CGCG | 1 cut(s) | 1699 |
| AciI | CCGC | 6 cut(s) | 118, 1085, 1697, 1699, 1950, 2265 |
| AcoI | YGGCCR | 5 cut(s) | 578, 1608, 1873, 2154, 2230 |
| AcsI | RAATTY | 6 cut(s) | 955, 1023, 1427, 1481, 1638, 1865 |
| AcuI | CTGAAG | 2 cut(s) | 1290, 2478 |
| AfaI | GTAC | 5 cut(s) | 407, 552, 1083, 1706, 2166 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 325, 620, 1685 |
| AflIII | ACRYGT | 1 cut(s) | 2116 |
| AjiI | CACGTC | 1 cut(s) | 1036 |
| AjnI | CCWGG | 5 cut(s) | 661, 1810, 1966, 2452, 2460 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 40, 72, 1148, 1180, 1320, 1352 |
| AloI | GAACNNNNNNTCC | 6 cut(s) | 242, 274, 619, 651, 1652, 1684 |
| AluBI | AGCT | 9 cut(s) | 368, 698, 916, 1537, 1785, 1884, 2111, 2270, 2498 |
| AluI | AGCT | 9 cut(s) | 368, 698, 916, 1537, 1785, 1884, 2111, 2270, 2498 |
| Alw21I | GWGCWC | 1 cut(s) | 294 |
| Alw26I | GTCTC | 3 cut(s) | 698, 1203, 2129 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 559 |
| Ama87I | CYCGRG | 1 cut(s) | 1182 |
| AoxI | GGCC | 8 cut(s) | 578, 966, 1114, 1608, 1795, 1873, 2154, 2230 |
| ApeKI | GCWGC | 6 cut(s) | 284, 1727, 1782, 2183, 2267, 2390 |
| ApoI | RAATTY | 6 cut(s) | 955, 1023, 1427, 1481, 1638, 1865 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1929, 2118 |
| AspLEI | GCGC | 3 cut(s) | 1810, 2368, 2440 |
| AspS9I | GGNCC | 3 cut(s) | 1114, 1220, 1796 |
| AsuC2I | CCSGG | 1 cut(s) | 326 |
| AsuHPI | GGTGA | 6 cut(s) | 36, 107, 147, 227, 591, 1982 |
| AsuII | TTCGAA | 1 cut(s) | 248 |
| AvaI | CYCGRG | 1 cut(s) | 1182 |
| AvaII | GGWCC | 1 cut(s) | 1220 |
| AxyI | CCTNAGG | 1 cut(s) | 1656 |
| BaeGI | GKGCMC | 1 cut(s) | 387 |
| BalI | TGGCCA | 5 cut(s) | 580, 1610, 1875, 2156, 2232 |
| BamHI | GGATCC | 2 cut(s) | 804, 2456 |
| BanI | GGYRCC | 1 cut(s) | 4 |
| BanII | GRGCYC | 1 cut(s) | 2025 |
| BbsI | GAAGAC | 3 cut(s) | 664, 1335, 2352 |
| Bbv12I | GWGCWC | 1 cut(s) | 294 |
| BbvI | GCAGC | 6 cut(s) | 271, 1739, 1794, 2170, 2279, 2377 |
| BccI | CCATC | 7 cut(s) | 395, 613, 794, 1220, 1619, 1807, 2006 |
| BceAI | ACGGC | 1 cut(s) | 1810 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 257, 291, 1751, 1785 |
| BciT130I | CCWGG | 5 cut(s) | 663, 1812, 1968, 2454, 2462 |
| BciVI | GTATCC | 1 cut(s) | 348 |
| BclI | TGATCA | 2 cut(s) | 1108, 2046 |
| BcnI | CCSGG | 1 cut(s) | 326 |
| BcoDI | GTCTC | 3 cut(s) | 698, 1203, 2129 |
| BfaI | CTAG | 6 cut(s) | 897, 1136, 1314, 1889, 1980, 2241 |
| BfmI | CTRYAG | 2 cut(s) | 2184, 2391 |
| BfoI | RGCGCY | 1 cut(s) | 1811 |
| BfuAI | ACCTGC | 1 cut(s) | 770 |
| BfuI | GTATCC | 1 cut(s) | 348 |
| BisI | GCNGC | 8 cut(s) | 285, 1728, 1783, 1951, 2184, 2265, 2268, 2391 |
| BlpI | GCTNAGC | 1 cut(s) | 834 |
| BlsI | GCNGC | 8 cut(s) | 286, 1729, 1784, 1952, 2185, 2266, 2269, 2392 |
| Bme1390I | CCNGG | 6 cut(s) | 326, 663, 1812, 1968, 2454, 2462 |
| Bme18I | GGWCC | 1 cut(s) | 1220 |
| BmeT110I | CYCGRG | 1 cut(s) | 1182 |
| BmgBI | CACGTC | 1 cut(s) | 1036 |
| BmgT120I | GGNCC | 3 cut(s) | 1114, 1220, 1796 |
| BmiI | GGNNCC | 4 cut(s) | 6, 736, 806, 2458 |
| BmrFI | CCNGG | 6 cut(s) | 326, 663, 1812, 1968, 2454, 2462 |
| BpiI | GAAGAC | 3 cut(s) | 664, 1335, 2352 |
| BpmI | CTGGAG | 1 cut(s) | 2483 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 834 |
| Bpu14I | TTCGAA | 1 cut(s) | 248 |
| BpuMI | CCSGG | 1 cut(s) | 326 |
| BsaBI | GATNNNNATC | 2 cut(s) | 336, 2065 |
| BsaI | GGTCTC | 1 cut(s) | 698 |
| BsaJI | CCNNGG | 9 cut(s) | 216, 228, 341, 1470, 1697, 1966, 2157, 2452, 2460 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 428, 458, 1652, 1682 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 325, 620, 1685 |
| Bse118I | RCCGGY | 1 cut(s) | 1492 |
| Bse1I | ACTGG | 4 cut(s) | 26, 902, 955, 1611 |
| Bse21I | CCTNAGG | 1 cut(s) | 1656 |
| Bse3DI | GCAATG | 3 cut(s) | 462, 1010, 2361 |
| Bse8I | GATNNNNATC | 2 cut(s) | 336, 2065 |
| BseBI | CCWGG | 5 cut(s) | 663, 1812, 1968, 2454, 2462 |
| BseDI | CCNNGG | 9 cut(s) | 216, 228, 341, 1470, 1697, 1966, 2157, 2452, 2460 |
| BseGI | GGATG | 7 cut(s) | 460, 721, 1072, 1192, 1277, 1582, 1927 |
| BseJI | GATNNNNATC | 2 cut(s) | 336, 2065 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 325, 620, 1685 |
| BseMI | GCAATG | 3 cut(s) | 462, 1010, 2361 |
| BseMII | CTCAG | 4 cut(s) | 825, 1193, 1629, 2082 |
| BseNI | ACTGG | 4 cut(s) | 26, 902, 955, 1611 |
| BseRI | GAGGAG | 3 cut(s) | 57, 142, 1219 |
| BseSI | GKGCMC | 1 cut(s) | 387 |
| BseXI | GCAGC | 6 cut(s) | 271, 1739, 1794, 2170, 2279, 2377 |
| BsgI | GTGCAG | 1 cut(s) | 798 |
| Bsh1236I | CGCG | 1 cut(s) | 1699 |
| BshFI | GGCC | 8 cut(s) | 580, 968, 1116, 1610, 1797, 1875, 2156, 2232 |
| BshNI | GGYRCC | 1 cut(s) | 4 |
| BsiHKAI | GWGCWC | 1 cut(s) | 294 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1182 |
| BsiSI | CCGG | 2 cut(s) | 326, 1493 |
| BslFI | GGGAC | 2 cut(s) | 282, 2474 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 325, 620, 1685 |
| BsmAI | GTCTC | 3 cut(s) | 698, 1203, 2129 |
| BsmFI | GGGAC | 2 cut(s) | 282, 2474 |
| BsmI | GAATGC | 2 cut(s) | 820, 1298 |
| BsnI | GGCC | 8 cut(s) | 580, 968, 1116, 1610, 1797, 1875, 2156, 2232 |
| Bso31I | GGTCTC | 1 cut(s) | 698 |
| BsoBI | CYCGRG | 1 cut(s) | 1182 |
| Bsp119I | TTCGAA | 1 cut(s) | 248 |
| Bsp1286I | GDGCHC | 3 cut(s) | 294, 387, 2025 |
| Bsp1407I | TGTACA | 1 cut(s) | 550 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 834 |
| Bsp19I | CCATGG | 3 cut(s) | 228, 341, 2157 |
| BspACI | CCGC | 6 cut(s) | 118, 1085, 1697, 1699, 1950, 2265 |
| BspANI | GGCC | 8 cut(s) | 580, 968, 1116, 1610, 1797, 1875, 2156, 2232 |
| BspCNI | CTCAG | 4 cut(s) | 826, 1194, 1628, 2083 |
| BspFNI | CGCG | 1 cut(s) | 1699 |
| BspHI | TCATGA | 1 cut(s) | 2008 |
| BspLI | GGNNCC | 4 cut(s) | 6, 736, 806, 2458 |
| BspMAI | CTGCAG | 2 cut(s) | 2188, 2395 |
| BspMI | ACCTGC | 1 cut(s) | 770 |
| BspT104I | TTCGAA | 1 cut(s) | 248 |
| BspT107I | GGYRCC | 1 cut(s) | 4 |
| BspTNI | GGTCTC | 1 cut(s) | 698 |
| BsrDI | GCAATG | 3 cut(s) | 462, 1010, 2361 |
| BsrFI | RCCGGY | 1 cut(s) | 1492 |
| BsrGI | TGTACA | 1 cut(s) | 550 |
| BsrI | ACTGG | 4 cut(s) | 26, 902, 955, 1611 |
| BssAI | RCCGGY | 1 cut(s) | 1492 |
| BssECI | CCNNGG | 9 cut(s) | 216, 228, 341, 1470, 1697, 1966, 2157, 2452, 2460 |
| BssNAI | GTATAC | 1 cut(s) | 538 |
| BssT1I | CCWWGG | 4 cut(s) | 216, 228, 341, 2157 |
| Bst1107I | GTATAC | 1 cut(s) | 538 |
| Bst2UI | CCWGG | 5 cut(s) | 663, 1812, 1968, 2454, 2462 |
| Bst4CI | ACNGT | 8 cut(s) | 296, 589, 753, 795, 1259, 1394, 2200, 2299 |
| Bst6I | CTCTTC | 5 cut(s) | 58, 79, 180, 345, 2322 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1250, 2273 |
| BstAUI | TGTACA | 1 cut(s) | 550 |
| BstBI | TTCGAA | 1 cut(s) | 248 |
| BstC8I | GCNNGC | 5 cut(s) | 578, 1076, 1529, 1886, 2262 |
| BstDEI | CTNAG | 5 cut(s) | 834, 1202, 1615, 1656, 2091 |
| BstDSI | CCRYGG | 4 cut(s) | 228, 341, 1697, 2157 |
| BstF5I | GGATG | 7 cut(s) | 460, 721, 1072, 1192, 1277, 1582, 1927 |
| BstFNI | CGCG | 1 cut(s) | 1699 |
| BstH2I | RGCGCY | 1 cut(s) | 1811 |
| BstHHI | GCGC | 3 cut(s) | 1810, 2368, 2440 |
| BstMAI | GTCTC | 3 cut(s) | 698, 1203, 2129 |
| BstNI | CCWGG | 5 cut(s) | 663, 1812, 1968, 2454, 2462 |
| BstNSI | RCATGY | 5 cut(s) | 417, 1082, 2120, 2194, 2260 |
| BstSCI | CCNGG | 6 cut(s) | 324, 661, 1810, 1966, 2452, 2460 |
| BstSFI | CTRYAG | 2 cut(s) | 2184, 2391 |
| BstSLI | GKGCMC | 1 cut(s) | 387 |
| BstUI | CGCG | 1 cut(s) | 1699 |
| BstV1I | GCAGC | 6 cut(s) | 271, 1739, 1794, 2170, 2279, 2377 |
| BstV2I | GAAGAC | 3 cut(s) | 664, 1335, 2352 |
| BstX2I | RGATCY | 5 cut(s) | 804, 878, 1855, 2311, 2456 |
| BstYI | RGATCY | 5 cut(s) | 804, 878, 1855, 2311, 2456 |
| BstZ17I | GTATAC | 1 cut(s) | 538 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1656 |
| BsuI | GTATCC | 1 cut(s) | 348 |
| BsuRI | GGCC | 8 cut(s) | 580, 968, 1116, 1610, 1797, 1875, 2156, 2232 |
| BtgI | CCRYGG | 4 cut(s) | 228, 341, 1697, 2157 |
| BtrI | CACGTC | 1 cut(s) | 1036 |
| BtsCI | GGATG | 7 cut(s) | 460, 721, 1072, 1192, 1277, 1582, 1927 |
| BtsI | GCAGTG | 1 cut(s) | 2440 |
| BtsIMutI | CAGTG | 4 cut(s) | 581, 1264, 2196, 2440 |
| BveI | ACCTGC | 1 cut(s) | 770 |
| Cac8I | GCNNGC | 5 cut(s) | 578, 1076, 1529, 1886, 2262 |
| CaiI | CAGNNNCTG | 1 cut(s) | 559 |
| CciI | TCATGA | 1 cut(s) | 2008 |
| CfoI | GCGC | 3 cut(s) | 1810, 2368, 2440 |
| Cfr10I | RCCGGY | 1 cut(s) | 1492 |
| Cfr13I | GGNCC | 3 cut(s) | 1114, 1220, 1796 |
| Cfr42I | CCGCGG | 1 cut(s) | 1700 |
| CseI | GACGC | 1 cut(s) | 812 |
| Csp6I | GTAC | 5 cut(s) | 406, 551, 1082, 1705, 2165 |
| CviQI | GTAC | 5 cut(s) | 406, 551, 1082, 1705, 2165 |
| DdeI | CTNAG | 5 cut(s) | 834, 1202, 1615, 1656, 2091 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2402 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2402 |
| EaeI | YGGCCR | 5 cut(s) | 578, 1608, 1873, 2154, 2230 |
| Eam1104I | CTCTTC | 5 cut(s) | 58, 79, 180, 345, 2322 |
| EarI | CTCTTC | 5 cut(s) | 58, 79, 180, 345, 2322 |
| Eco130I | CCWWGG | 4 cut(s) | 216, 228, 341, 2157 |
| Eco24I | GRGCYC | 1 cut(s) | 2025 |
| Eco31I | GGTCTC | 1 cut(s) | 698 |
| Eco47I | GGWCC | 1 cut(s) | 1220 |
| Eco57I | CTGAAG | 2 cut(s) | 1290, 2478 |
| Eco81I | CCTNAGG | 1 cut(s) | 1656 |
| Eco88I | CYCGRG | 1 cut(s) | 1182 |
| EcoRI | GAATTC | 2 cut(s) | 1023, 1427 |
| EcoRII | CCWGG | 5 cut(s) | 661, 1810, 1966, 2452, 2460 |
| EcoT14I | CCWWGG | 4 cut(s) | 216, 228, 341, 2157 |
| EcoT22I | ATGCAT | 3 cut(s) | 1010, 1174, 2278 |
| EcoT38I | GRGCYC | 1 cut(s) | 2025 |
| ErhI | CCWWGG | 4 cut(s) | 216, 228, 341, 2157 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1320, 1352, 1658, 1690 |
| FaqI | GGGAC | 2 cut(s) | 282, 2474 |
| FauI | CCCGC | 1 cut(s) | 1692 |
| FauNDI | CATATG | 1 cut(s) | 2281 |
| FbaI | TGATCA | 2 cut(s) | 1108, 2046 |
| FblI | GTMKAC | 3 cut(s) | 537, 1131, 1558 |
| Fnu4HI | GCNGC | 8 cut(s) | 285, 1728, 1783, 1951, 2184, 2265, 2268, 2391 |
| FokI | GGATG | 7 cut(s) | 467, 728, 1079, 1199, 1264, 1589, 1934 |
| FriOI | GRGCYC | 1 cut(s) | 2025 |
| Fsp4HI | GCNGC | 8 cut(s) | 285, 1728, 1783, 1951, 2184, 2265, 2268, 2391 |
| FspBI | CTAG | 6 cut(s) | 897, 1136, 1314, 1889, 1980, 2241 |
| GlaI | GCGC | 3 cut(s) | 1809, 2367, 2439 |
| GluI | GCNGC | 8 cut(s) | 285, 1728, 1783, 1951, 2184, 2265, 2268, 2391 |
| GsuI | CTGGAG | 1 cut(s) | 2483 |
| HaeII | RGCGCY | 1 cut(s) | 1811 |
| HaeIII | GGCC | 8 cut(s) | 580, 968, 1116, 1610, 1797, 1875, 2156, 2232 |
| HapII | CCGG | 2 cut(s) | 326, 1493 |
| HgaI | GACGC | 1 cut(s) | 812 |
| HhaI | GCGC | 3 cut(s) | 1810, 2368, 2440 |
| Hin6I | GCGC | 3 cut(s) | 1808, 2366, 2438 |
| HinP1I | GCGC | 3 cut(s) | 1808, 2366, 2438 |
| HincII | GTYRAC | 2 cut(s) | 771, 1559 |
| HindII | GTYRAC | 2 cut(s) | 771, 1559 |
| HindIII | AAGCTT | 2 cut(s) | 696, 1535 |
| HinfI | GANTC | 5 cut(s) | 443, 857, 910, 1265, 2380 |
| HpaII | CCGG | 2 cut(s) | 326, 1493 |
| HphI | GGTGA | 6 cut(s) | 36, 107, 147, 227, 591, 1982 |
| Hpy166II | GTNNAC | 5 cut(s) | 538, 771, 996, 1132, 1559 |
| Hpy8I | GTNNAC | 5 cut(s) | 538, 771, 996, 1132, 1559 |
| Hpy99I | CGWCG | 1 cut(s) | 1040 |
| HpyCH4III | ACNGT | 8 cut(s) | 296, 589, 753, 795, 1259, 1394, 2200, 2299 |
| HpyCH4IV | ACGT | 2 cut(s) | 429, 1035 |
| HpyF3I | CTNAG | 5 cut(s) | 834, 1202, 1615, 1656, 2091 |
| HpySE526I | ACGT | 2 cut(s) | 429, 1035 |
| HspAI | GCGC | 3 cut(s) | 1808, 2366, 2438 |
| Ksp22I | TGATCA | 2 cut(s) | 1108, 2046 |
| KspI | CCGCGG | 1 cut(s) | 1700 |
| LmnI | GCTCC | 2 cut(s) | 734, 1295 |
| Lsp1109I | GCAGC | 6 cut(s) | 271, 1739, 1794, 2170, 2279, 2377 |
| MaeI | CTAG | 6 cut(s) | 897, 1136, 1314, 1889, 1980, 2241 |
| MaeII | ACGT | 2 cut(s) | 429, 1035 |
| MaeIII | GTNAC | 4 cut(s) | 113, 425, 1388, 1394 |
| MfeI | CAATTG | 3 cut(s) | 399, 744, 1899 |
| MflI | RGATCY | 5 cut(s) | 804, 878, 1855, 2311, 2456 |
| MhlI | GDGCHC | 3 cut(s) | 294, 387, 2025 |
| MlsI | TGGCCA | 5 cut(s) | 580, 1610, 1875, 2156, 2232 |
| MluNI | TGGCCA | 5 cut(s) | 580, 1610, 1875, 2156, 2232 |
| MlyI | GAGTC | 1 cut(s) | 452 |
| MmeI | TCCRAC | 3 cut(s) | 921, 1018, 2339 |
| Mox20I | TGGCCA | 5 cut(s) | 580, 1610, 1875, 2156, 2232 |
| Mph1103I | ATGCAT | 3 cut(s) | 1010, 1174, 2278 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1929, 2118 |
| MscI | TGGCCA | 5 cut(s) | 580, 1610, 1875, 2156, 2232 |
| MseI | TTAA | 5 cut(s) | 669, 1359, 1959, 2144, 2171 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1575, 2156 |
| Msp20I | TGGCCA | 5 cut(s) | 580, 1610, 1875, 2156, 2232 |
| MspA1I | CMGCKG | 3 cut(s) | 916, 1699, 1884 |
| MspI | CCGG | 2 cut(s) | 326, 1493 |
| MspR9I | CCNGG | 6 cut(s) | 326, 663, 1812, 1968, 2454, 2462 |
| MunI | CAATTG | 3 cut(s) | 399, 744, 1899 |
| Mva1269I | GAATGC | 2 cut(s) | 820, 1298 |
| MvaI | CCWGG | 5 cut(s) | 663, 1812, 1968, 2454, 2462 |
| MvnI | CGCG | 1 cut(s) | 1699 |
| NciI | CCSGG | 1 cut(s) | 326 |
| NcoI | CCATGG | 3 cut(s) | 228, 341, 2157 |
| NdeI | CATATG | 1 cut(s) | 2281 |
| NlaIV | GGNNCC | 4 cut(s) | 6, 736, 806, 2458 |
| NmeAIII | GCCGAG | 1 cut(s) | 313 |
| NmuCI | GTSAC | 3 cut(s) | 113, 425, 1394 |
| NsiI | ATGCAT | 3 cut(s) | 1010, 1174, 2278 |
| NspI | RCATGY | 5 cut(s) | 417, 1082, 2120, 2194, 2260 |
| NspV | TTCGAA | 1 cut(s) | 248 |
| PagI | TCATGA | 1 cut(s) | 2008 |
| PciI | ACATGT | 1 cut(s) | 2116 |
| PctI | GAATGC | 2 cut(s) | 820, 1298 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1929, 2118 |
| PfeI | GAWTC | 4 cut(s) | 857, 910, 1265, 2380 |
| PkrI | GCNGC | 8 cut(s) | 286, 1729, 1784, 1952, 2185, 2266, 2269, 2392 |
| PleI | GAGTC | 1 cut(s) | 451 |
| PpsI | GAGTC | 1 cut(s) | 451 |
| PscI | ACATGT | 1 cut(s) | 2116 |
| Psp6I | CCWGG | 5 cut(s) | 661, 1810, 1966, 2452, 2460 |
| PspGI | CCWGG | 5 cut(s) | 661, 1810, 1966, 2452, 2460 |
| PspN4I | GGNNCC | 4 cut(s) | 6, 736, 806, 2458 |
| PspPI | GGNCC | 3 cut(s) | 1114, 1220, 1796 |
| PstI | CTGCAG | 2 cut(s) | 2188, 2395 |
| PstNI | CAGNNNCTG | 1 cut(s) | 559 |
| PsuI | RGATCY | 5 cut(s) | 804, 878, 1855, 2311, 2456 |
| PvuII | CAGCTG | 2 cut(s) | 916, 1884 |
| RsaI | GTAC | 5 cut(s) | 407, 552, 1083, 1706, 2166 |
| RsaNI | GTAC | 5 cut(s) | 406, 551, 1082, 1705, 2165 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1575, 2156 |
| SacII | CCGCGG | 1 cut(s) | 1700 |
| SalI | GTCGAC | 1 cut(s) | 1557 |
| SaqAI | TTAA | 5 cut(s) | 669, 1359, 1959, 2144, 2171 |
| SatI | GCNGC | 8 cut(s) | 285, 1728, 1783, 1951, 2184, 2265, 2268, 2391 |
| Sau96I | GGNCC | 3 cut(s) | 1114, 1220, 1796 |
| SchI | GAGTC | 1 cut(s) | 452 |
| ScrFI | CCNGG | 6 cut(s) | 326, 663, 1812, 1968, 2454, 2462 |
| SduI | GDGCHC | 3 cut(s) | 294, 387, 2025 |
| SfcI | CTRYAG | 2 cut(s) | 2184, 2391 |
| Sfr303I | CCGCGG | 1 cut(s) | 1700 |
| SfuI | TTCGAA | 1 cut(s) | 248 |
| SgrBI | CCGCGG | 1 cut(s) | 1700 |
| SinI | GGWCC | 1 cut(s) | 1220 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1575, 2156 |
| SsiI | CCGC | 6 cut(s) | 118, 1085, 1697, 1699, 1950, 2265 |
| SspI | AATATT | 1 cut(s) | 1929 |
| SspMI | CTAG | 6 cut(s) | 897, 1136, 1314, 1889, 1980, 2241 |
| StyD4I | CCNGG | 6 cut(s) | 324, 661, 1810, 1966, 2452, 2460 |
| StyI | CCWWGG | 4 cut(s) | 216, 228, 341, 2157 |
| TaaI | ACNGT | 8 cut(s) | 296, 589, 753, 795, 1259, 1394, 2200, 2299 |
| TaiI | ACGT | 2 cut(s) | 432, 1038 |
| TaqI | TCGA | 8 cut(s) | 199, 248, 370, 441, 1509, 1558, 1771, 2133 |
| TatI | WGTACW | 2 cut(s) | 550, 1704 |
| TauI | GCSGC | 2 cut(s) | 1953, 2267 |
| TfiI | GAWTC | 4 cut(s) | 857, 910, 1265, 2380 |
| Tru1I | TTAA | 5 cut(s) | 669, 1359, 1959, 2144, 2171 |
| Tru9I | TTAA | 5 cut(s) | 669, 1359, 1959, 2144, 2171 |
| TscAI | CASTG | 4 cut(s) | 588, 1264, 2203, 2440 |
| TseFI | GTSAC | 3 cut(s) | 113, 425, 1394 |
| TseI | GCWGC | 6 cut(s) | 284, 1727, 1782, 2183, 2267, 2390 |
| Tsp45I | GTSAC | 3 cut(s) | 113, 425, 1394 |
| TspDTI | ATGAA | 7 cut(s) | 112, 471, 987, 1440, 1665, 1878, 1938 |
| TspRI | CASTG | 4 cut(s) | 588, 1264, 2203, 2440 |
| VpaK11BI | GGWCC | 1 cut(s) | 1220 |
| XapI | RAATTY | 6 cut(s) | 955, 1023, 1427, 1481, 1638, 1865 |
| XceI | RCATGY | 5 cut(s) | 417, 1082, 2120, 2194, 2260 |
| XmiI | GTMKAC | 3 cut(s) | 537, 1131, 1558 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1929, 2118 |
| XspI | CTAG | 6 cut(s) | 897, 1136, 1314, 1889, 1980, 2241 |
| Zsp2I | ATGCAT | 3 cut(s) | 1010, 1174, 2278 |