MD03G1291600.v1.1

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015856
Physical Location & Seq
Reverse (-)
36908229 .. 36909214
986 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1291600.v1.1.491

Sequence Viewer

Length: 576 bp
ATGGCGGTTTTTTATCACCATTTCTTGCATGCAACCTTTTTGCTAGTCTTGGTACTGGTGGCAGTGACGGCTTCCGCGGCTGGTGCGGGCGGCTACGGCAACACAAATCCTGATCAGTATGAGAAGCCCAAACCAGAAAAACAGAACAACCTACTATCAACCAATGCCATTTTGGGCATTCAAGGACTTGTTTATTGCAAATCTGGCCATGAAGTCCTTCCGCTCGAAGGAGCGGCAGCCAGGATAACGTGCGAGGCTGTAGACCAATATGGGTTGGAAACTTCACCAGTCACCATCTTAGGTGATGCAACTGACGCAAAGGGTTATTTCTTTGCAACAATATGTCCTTCTGAGATTGAAAACAAGAAGCAGCTCACTAAGTGCAAAGCCTTTCTTGAACTCTCTTCGTCGGAGACTTGCAACATTCCGACGGACTCGAACAGCGGAATCAGTGGTGCTGTGCTAGCTTCATATCGCCTCCTCCATGACAAGAACATCAAGCTATATACTGTAGGGCCTTTTACTTTCACATCATCACCACAAACCCAGAGTTCAGTCCCTAATAATGGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

192

Amino Acids

20.44

Weight (kDa)

5.92

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 60 - 152 1.5e-18 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015856)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47540
fragaria_vesca FvH4_2g26050
malus_domestica MD03G1291600.v1.1
prunus_persica Prupe.8G264700_v2.0.a1
pyrus_communis pycom03g23100
rosa_chinensis RchiOBHm_Chr6g0294411
rosa_laevigata RLG00000011872
rosa_multiflora Rmu_sc0026241.1_g000001
rosa_roxburghii Rroxscaffold_7G00173390
rosa_rugosa Rorug06G0244500
rosa_samantha Rh6AG356900 Rh6BG363600 Rh6CG370900 Rh6DG357600
rosa_wichuraiana Rw6G031110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 223, 233
AccI GTMKAC 1 cut(s) 261
AccII CGCG 1 cut(s) 77
AciI CCGC 8 cut(s) 5, 75, 77, 86, 90, 221, 233, 444
AcoI YGGCCR 1 cut(s) 205
AdeI CACNNNGTG 1 cut(s) 381
AfaI GTAC 1 cut(s) 54
AfiI CCNNNNNNNGG 2 cut(s) 227, 566
AgsI TTSAA 3 cut(s) 182, 359, 398
AjnI CCWGG 1 cut(s) 239
AluBI AGCT 3 cut(s) 373, 467, 502
AluI AGCT 3 cut(s) 373, 467, 502
Alw26I GTCTC 1 cut(s) 407
AoxI GGCC 2 cut(s) 205, 515
ApeKI GCWGC 2 cut(s) 236, 370
Asp700I GAANNNNTTC 1 cut(s) 216
AspS9I GGNCC 1 cut(s) 515
AsuHPI GGTGA 5 cut(s) 8, 276, 283, 314, 528
AsuNHI GCTAGC 1 cut(s) 463
BalI TGGCCA 1 cut(s) 207
BbvI GCAGC 2 cut(s) 248, 382
BccI CCATC 1 cut(s) 302
BceAI ACGGC 2 cut(s) 84, 112
BciT130I CCWGG 1 cut(s) 241
BclI TGATCA 1 cut(s) 112
BcoDI GTCTC 1 cut(s) 407
BfaI CTAG 2 cut(s) 44, 464
BfmI CTRYAG 2 cut(s) 258, 510
BisI GCNGC 5 cut(s) 78, 91, 234, 237, 371
BlsI GCNGC 5 cut(s) 79, 92, 235, 238, 372
Bme1390I CCNGG 1 cut(s) 241
BmgT120I GGNCC 1 cut(s) 515
BmrFI CCNGG 1 cut(s) 241
BmsI GCATC 1 cut(s) 295
BmtI GCTAGC 1 cut(s) 467
BsaJI CCNNGG 1 cut(s) 75
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 566
Bse1I ACTGG 2 cut(s) 60, 287
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 227, 566
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 2 cut(s) 60, 287
BseRI GAGGAG 1 cut(s) 470
BseXI GCAGC 2 cut(s) 248, 382
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 2 cut(s) 207, 517
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 2 cut(s) 227, 566
BsmAI GTCTC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 542
BsmI GAATGC 1 cut(s) 177
BsnI GGCC 2 cut(s) 207, 517
Bsp143I GATC 1 cut(s) 112
BspACI CCGC 8 cut(s) 5, 75, 77, 86, 90, 221, 233, 444
BspANI GGCC 2 cut(s) 207, 517
BspCNI CTCAG 1 cut(s) 343
BspFNI CGCG 1 cut(s) 77
BspOI GCTAGC 1 cut(s) 467
BsrBI CCGCTC 2 cut(s) 223, 233
BsrI ACTGG 2 cut(s) 60, 287
BssECI CCNNGG 1 cut(s) 75
BssMI GATC 1 cut(s) 112
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 511
Bst6I CTCTTC 1 cut(s) 409
BstC8I GCNNGC 3 cut(s) 30, 88, 465
BstDEI CTNAG 3 cut(s) 298, 351, 378
BstDSI CCRYGG 1 cut(s) 75
BstFNI CGCG 1 cut(s) 77
BstKTI GATC 1 cut(s) 115
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 1 cut(s) 112
BstMWI GCNNNNNNNGC 7 cut(s) 68, 77, 83, 96, 204, 314, 464
BstNI CCWGG 1 cut(s) 241
BstNSI RCATGY 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 239
BstSFI CTRYAG 2 cut(s) 258, 510
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 2 cut(s) 248, 382
BsuRI GGCC 2 cut(s) 207, 517
BtgI CCRYGG 1 cut(s) 75
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 457
Cac8I GCNNGC 3 cut(s) 30, 88, 465
Cfr13I GGNCC 1 cut(s) 515
Cfr42I CCGCGG 1 cut(s) 78
CseI GACGC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 53
CviAII CATG 3 cut(s) 29, 209, 485
CviQI GTAC 1 cut(s) 53
DdeI CTNAG 3 cut(s) 298, 351, 378
DpnI GATC 1 cut(s) 114
DpnII GATC 1 cut(s) 112
DraIII CACNNNGTG 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 409
EarI CTCTTC 1 cut(s) 409
EcoO109I RGGNCCY 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 239
FaeI CATG 3 cut(s) 32, 212, 488
FaiI YATR 9 cut(s) 30, 120, 210, 270, 343, 472, 486, 505, 507
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 1 cut(s) 542
FatI CATG 3 cut(s) 28, 208, 484
FauI CCCGC 1 cut(s) 79
FbaI TGATCA 1 cut(s) 112
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 5 cut(s) 78, 91, 234, 237, 371
Fsp4HI GCNGC 5 cut(s) 78, 91, 234, 237, 371
FspBI CTAG 2 cut(s) 44, 464
GluI GCNGC 5 cut(s) 78, 91, 234, 237, 371
HaeIII GGCC 2 cut(s) 207, 517
HgaI GACGC 1 cut(s) 323
Hin1II CATG 3 cut(s) 32, 212, 488
HinfI GANTC 2 cut(s) 434, 447
HphI GGTGA 5 cut(s) 8, 276, 283, 314, 528
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 3 cut(s) 352, 412, 429
Hpy188III TCNNGA 2 cut(s) 110, 395
Hpy8I GTNNAC 1 cut(s) 262
Hpy99I CGWCG 2 cut(s) 412, 433
HpyAV CCTTC 3 cut(s) 221, 227, 357
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4IV ACGT 1 cut(s) 248
HpyCH4V TGCA 7 cut(s) 28, 32, 198, 308, 335, 384, 420
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 77, 83, 96, 204, 314, 464
HpyF3I CTNAG 3 cut(s) 298, 351, 378
HpySE526I ACGT 1 cut(s) 248
Hsp92II CATG 3 cut(s) 32, 212, 488
Ksp22I TGATCA 1 cut(s) 112
KspI CCGCGG 1 cut(s) 78
Kzo9I GATC 1 cut(s) 112
LmnI GCTCC 1 cut(s) 230
LpnPI CCDG 9 cut(s) 41, 66, 123, 147, 189, 226, 253, 300, 560
Lsp1109I GCAGC 2 cut(s) 248, 382
LweI GCATC 1 cut(s) 295
MaeI CTAG 2 cut(s) 44, 464
MaeII ACGT 1 cut(s) 248
MaeIII GTNAC 2 cut(s) 64, 289
MalI GATC 1 cut(s) 114
MbiI CCGCTC 2 cut(s) 223, 233
MboI GATC 1 cut(s) 112
MboII GAAGA 1 cut(s) 396
MlsI TGGCCA 1 cut(s) 207
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 3 cut(s) 255, 390, 452
MnlI CCTC 3 cut(s) 247, 488, 491
Mox20I TGGCCA 1 cut(s) 207
MroXI GAANNNNTTC 1 cut(s) 216
MscI TGGCCA 1 cut(s) 207
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 2 cut(s) 77, 444
MspR9I CCNGG 1 cut(s) 241
Mva1269I GAATGC 1 cut(s) 177
MvaI CCWGG 1 cut(s) 241
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 7 cut(s) 68, 77, 83, 96, 204, 314, 464
NdeII GATC 1 cut(s) 112
NheI GCTAGC 1 cut(s) 463
NlaIII CATG 3 cut(s) 32, 212, 488
NmuCI GTSAC 2 cut(s) 64, 289
NspI RCATGY 1 cut(s) 32
PaeI GCATGC 1 cut(s) 32
PctI GAATGC 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 1 cut(s) 447
PkrI GCNGC 5 cut(s) 79, 92, 235, 238, 372
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
Psp6I CCWGG 1 cut(s) 239
PspGI CCWGG 1 cut(s) 239
PspPI GGNCC 1 cut(s) 515
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
SacII CCGCGG 1 cut(s) 78
SatI GCNGC 5 cut(s) 78, 91, 234, 237, 371
Sau3AI GATC 1 cut(s) 112
Sau96I GGNCC 1 cut(s) 515
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 1 cut(s) 241
SetI ASST 7 cut(s) 38, 153, 251, 304, 375, 469, 504
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 2 cut(s) 258, 510
Sfr303I CCGCGG 1 cut(s) 78
SgrBI CCGCGG 1 cut(s) 78
SphI GCATGC 1 cut(s) 32
SsiI CCGC 8 cut(s) 5, 75, 77, 86, 90, 221, 233, 444
SspMI CTAG 2 cut(s) 44, 464
StyD4I CCNGG 1 cut(s) 239
TaaI ACNGT 1 cut(s) 511
TaiI ACGT 1 cut(s) 251
TaqI TCGA 2 cut(s) 225, 437
TauI GCSGC 3 cut(s) 80, 93, 236
TfiI GAWTC 1 cut(s) 447
TscAI CASTG 2 cut(s) 69, 457
TseFI GTSAC 2 cut(s) 64, 289
TseI GCWGC 2 cut(s) 236, 370
Tsp45I GTSAC 2 cut(s) 64, 289
TspDTI ATGAA 2 cut(s) 225, 459
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 2 cut(s) 69, 457
XceI RCATGY 1 cut(s) 32
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 261
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 2 cut(s) 44, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.