pycom03g23100

Proline-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0015856
Physical Location & Seq
Reverse (-)
23057841 .. 23058514
674 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23100.2

Sequence Viewer

Length: 576 bp
ATGGCGGCTTTTAATCACCATTTCTTGCACGCAACCTCTTTGCTAGTCTTGGTACTGGTGGCAGTGACGGCTTCCGAGGCTGGTGCCGGCGGCTACGGCAACACAAATCCCGATCAGTATGAGAAGCCCAAACCAGAAAAACAGAACAACCTACTATCAACCAATGCCATTTTGGGCATTCAAGGCCTTGTTTATTGCAAATCCGGCCATGAAGTCCTCCCGCTCGAAGGAGCGGCAGCCAGGATTACGTGCGAGGCTGTAGACCAGTATGGATTGGAAACTTCACCTGTCACCATCTTAAGTGATGCAACTGACGCAAAGGGTTATTTCTTTGCAACAATATGTCCTTCTGAGATTGAAAACAAGAAGCAGCTCACTAAGTGCAAAGCCTTTCTTGAACTCTCTTCGTCCGAGACTTGCAACATTCCGACGGACTCGAACAGCGGAATCAGTGGTGCTGTGCTAGCTTCATATCGCCTCCTCCATGACAAGAACATCAAGCTATATACTGTAGGGCCTTTTACTTTCACATCATCACCACAAACCCAGAGTTCAGTCCCAAATAATGGCTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

192

Amino Acids

20.39

Weight (kDa)

5.69

Isoelectric Point (pI)

31.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015856)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47540
fragaria_vesca FvH4_2g26050
malus_domestica MD03G1291600.v1.1
prunus_persica Prupe.8G264700_v2.0.a1
pyrus_communis pycom03g23100
rosa_chinensis RchiOBHm_Chr6g0294411
rosa_laevigata RLG00000011872
rosa_multiflora Rmu_sc0026241.1_g000001
rosa_roxburghii Rroxscaffold_7G00173390
rosa_rugosa Rorug06G0244500
rosa_samantha Rh6AG356900 Rh6BG363600 Rh6CG370900 Rh6DG357600
rosa_wichuraiana Rw6G031110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AccB7I CCANNNNNTGG 1 cut(s) 566
AccBSI CCGCTC 2 cut(s) 223, 233
AccI GTMKAC 1 cut(s) 261
AciI CCGC 5 cut(s) 5, 90, 221, 233, 444
AcoI YGGCCR 1 cut(s) 205
AdeI CACNNNGTG 1 cut(s) 381
AfaI GTAC 1 cut(s) 54
AfiI CCNNNNNNNGG 2 cut(s) 227, 566
AflII CTTAAG 1 cut(s) 298
AgsI TTSAA 3 cut(s) 182, 359, 398
AjnI CCWGG 1 cut(s) 239
AluBI AGCT 3 cut(s) 373, 467, 502
AluI AGCT 3 cut(s) 373, 467, 502
Alw26I GTCTC 1 cut(s) 407
AoxI GGCC 3 cut(s) 184, 205, 515
ApeKI GCWGC 2 cut(s) 236, 370
AspS9I GGNCC 1 cut(s) 515
AsuHPI GGTGA 4 cut(s) 8, 276, 283, 528
AsuNHI GCTAGC 1 cut(s) 463
BanI GGYRCC 1 cut(s) 83
BbvI GCAGC 2 cut(s) 248, 382
BccI CCATC 1 cut(s) 302
BceAI ACGGC 2 cut(s) 84, 112
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BciT130I CCWGG 1 cut(s) 241
BcoDI GTCTC 1 cut(s) 407
BfaI CTAG 3 cut(s) 44, 464, 574
BfmI CTRYAG 2 cut(s) 258, 510
BfrI CTTAAG 1 cut(s) 298
BisI GCNGC 5 cut(s) 6, 91, 234, 237, 371
BlsI GCNGC 5 cut(s) 7, 92, 235, 238, 372
Bme1390I CCNGG 1 cut(s) 241
BmgT120I GGNCC 1 cut(s) 515
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 1 cut(s) 241
BmsI GCATC 1 cut(s) 295
BmtI GCTAGC 1 cut(s) 467
BsaAI YACGTR 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 75
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 566
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 60, 265
BseBI CCWGG 1 cut(s) 241
BseDI CCNNGG 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 227, 566
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 2 cut(s) 60, 265
BseRI GAGGAG 1 cut(s) 470
BseXI GCAGC 2 cut(s) 248, 382
BshFI GGCC 3 cut(s) 186, 207, 517
BshNI GGYRCC 1 cut(s) 83
BsiSI CCGG 2 cut(s) 87, 204
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 2 cut(s) 227, 566
BsmAI GTCTC 1 cut(s) 407
BsmFI GGGAC 1 cut(s) 542
BsmI GAATGC 1 cut(s) 177
BsnI GGCC 3 cut(s) 186, 207, 517
Bsp143I GATC 1 cut(s) 112
BspACI CCGC 5 cut(s) 5, 90, 221, 233, 444
BspANI GGCC 3 cut(s) 186, 207, 517
BspCNI CTCAG 1 cut(s) 343
BspLI GGNNCC 1 cut(s) 85
BspOI GCTAGC 1 cut(s) 467
BspT107I GGYRCC 1 cut(s) 83
BspTI CTTAAG 1 cut(s) 298
BsrBI CCGCTC 2 cut(s) 223, 233
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 2 cut(s) 60, 265
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 75
BssMI GATC 1 cut(s) 112
Bst2UI CCWGG 1 cut(s) 241
Bst4CI ACNGT 1 cut(s) 511
Bst6I CTCTTC 1 cut(s) 409
BstAFI CTTAAG 1 cut(s) 298
BstBAI YACGTR 1 cut(s) 249
BstC8I GCNNGC 3 cut(s) 30, 88, 465
BstDEI CTNAG 2 cut(s) 351, 378
BstKTI GATC 1 cut(s) 115
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 1 cut(s) 112
BstMWI GCNNNNNNNGC 7 cut(s) 68, 77, 96, 183, 204, 314, 464
BstNI CCWGG 1 cut(s) 241
BstSCI CCNGG 1 cut(s) 239
BstSFI CTRYAG 2 cut(s) 258, 510
BstV1I GCAGC 2 cut(s) 248, 382
BsuRI GGCC 3 cut(s) 186, 207, 517
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 2 cut(s) 69, 457
Cac8I GCNNGC 3 cut(s) 30, 88, 465
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 515
CseI GACGC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 53
CviAII CATG 2 cut(s) 209, 485
CviQI GTAC 1 cut(s) 53
DdeI CTNAG 2 cut(s) 351, 378
DpnI GATC 1 cut(s) 114
DpnII GATC 1 cut(s) 112
DraIII CACNNNGTG 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 409
EarI CTCTTC 1 cut(s) 409
Eco147I AGGCCT 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 515
EcoRII CCWGG 1 cut(s) 239
FaeI CATG 2 cut(s) 212, 488
FaiI YATR 8 cut(s) 120, 210, 270, 343, 472, 486, 505, 507
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FaqI GGGAC 1 cut(s) 542
FatI CATG 2 cut(s) 208, 484
FauI CCCGC 1 cut(s) 228
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 5 cut(s) 6, 91, 234, 237, 371
Fsp4HI GCNGC 5 cut(s) 6, 91, 234, 237, 371
FspBI CTAG 3 cut(s) 44, 464, 574
GluI GCNGC 5 cut(s) 6, 91, 234, 237, 371
HaeIII GGCC 3 cut(s) 186, 207, 517
HapII CCGG 2 cut(s) 87, 204
HgaI GACGC 1 cut(s) 323
Hin1II CATG 2 cut(s) 212, 488
HinfI GANTC 2 cut(s) 434, 447
HpaII CCGG 2 cut(s) 87, 204
HphI GGTGA 4 cut(s) 8, 276, 283, 528
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 4 cut(s) 76, 352, 412, 429
Hpy188III TCNNGA 2 cut(s) 110, 395
Hpy8I GTNNAC 1 cut(s) 262
Hpy99I CGWCG 1 cut(s) 433
HpyAV CCTTC 2 cut(s) 221, 357
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4IV ACGT 1 cut(s) 248
HpyCH4V TGCA 6 cut(s) 28, 198, 308, 335, 384, 420
HpyF10VI GCNNNNNNNGC 7 cut(s) 68, 77, 96, 183, 204, 314, 464
HpyF3I CTNAG 2 cut(s) 351, 378
HpySE526I ACGT 1 cut(s) 248
Hsp92II CATG 2 cut(s) 212, 488
KroI GCCGGC 1 cut(s) 86
KroNI GCCGGC 1 cut(s) 88
Kzo9I GATC 1 cut(s) 112
LmnI GCTCC 1 cut(s) 230
Lsp1109I GCAGC 2 cut(s) 248, 382
LweI GCATC 1 cut(s) 295
MaeI CTAG 3 cut(s) 44, 464, 574
MaeII ACGT 1 cut(s) 248
MaeIII GTNAC 2 cut(s) 64, 289
MalI GATC 1 cut(s) 114
MbiI CCGCTC 2 cut(s) 223, 233
MboI GATC 1 cut(s) 112
MboII GAAGA 1 cut(s) 396
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 1 cut(s) 452
MnlI CCTC 6 cut(s) 46, 70, 227, 247, 488, 491
MroNI GCCGGC 1 cut(s) 86
MseI TTAA 2 cut(s) 12, 299
MspA1I CMGCKG 1 cut(s) 444
MspCI CTTAAG 1 cut(s) 298
MspI CCGG 2 cut(s) 87, 204
MspR9I CCNGG 1 cut(s) 241
Mva1269I GAATGC 1 cut(s) 177
MvaI CCWGG 1 cut(s) 241
MwoI GCNNNNNNNGC 7 cut(s) 68, 77, 96, 183, 204, 314, 464
NaeI GCCGGC 1 cut(s) 88
NdeII GATC 1 cut(s) 112
NgoMIV GCCGGC 1 cut(s) 86
NheI GCTAGC 1 cut(s) 463
NlaIII CATG 2 cut(s) 212, 488
NlaIV GGNNCC 1 cut(s) 85
NmuCI GTSAC 2 cut(s) 64, 289
PceI AGGCCT 1 cut(s) 186
PctI GAATGC 1 cut(s) 177
PdiI GCCGGC 1 cut(s) 88
PfeI GAWTC 1 cut(s) 447
PflMI CCANNNNNTGG 1 cut(s) 566
PkrI GCNGC 5 cut(s) 7, 92, 235, 238, 372
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
Ppu21I YACGTR 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 239
PspGI CCWGG 1 cut(s) 239
PspN4I GGNNCC 1 cut(s) 85
PspPI GGNCC 1 cut(s) 515
RsaI GTAC 1 cut(s) 54
RsaNI GTAC 1 cut(s) 53
SaqAI TTAA 2 cut(s) 12, 299
SatI GCNGC 5 cut(s) 6, 91, 234, 237, 371
Sau3AI GATC 1 cut(s) 112
Sau96I GGNCC 1 cut(s) 515
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 1 cut(s) 241
SetI ASST 7 cut(s) 38, 153, 251, 289, 375, 469, 504
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 2 cut(s) 258, 510
SmlI CTYRAG 1 cut(s) 298
SmoI CTYRAG 1 cut(s) 298
SseBI AGGCCT 1 cut(s) 186
SsiI CCGC 5 cut(s) 5, 90, 221, 233, 444
SspMI CTAG 3 cut(s) 44, 464, 574
StuI AGGCCT 1 cut(s) 186
StyD4I CCNGG 1 cut(s) 239
TaaI ACNGT 1 cut(s) 511
TaiI ACGT 1 cut(s) 251
TaqI TCGA 2 cut(s) 225, 437
TauI GCSGC 3 cut(s) 8, 93, 236
TfiI GAWTC 1 cut(s) 447
Tru1I TTAA 2 cut(s) 12, 299
Tru9I TTAA 2 cut(s) 12, 299
TscAI CASTG 2 cut(s) 69, 457
TseFI GTSAC 2 cut(s) 64, 289
TseI GCWGC 2 cut(s) 236, 370
Tsp45I GTSAC 2 cut(s) 64, 289
TspDTI ATGAA 2 cut(s) 225, 459
TspGWI ACGGA 1 cut(s) 446
TspRI CASTG 2 cut(s) 69, 457
Van91I CCANNNNNTGG 1 cut(s) 566
Vha464I CTTAAG 1 cut(s) 298
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 3 cut(s) 44, 464, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.